CN110794156A - Measuring chamber for full-automatic chemiluminescence immunoassay analyzer - Google Patents
Measuring chamber for full-automatic chemiluminescence immunoassay analyzer Download PDFInfo
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- CN110794156A CN110794156A CN201911209314.1A CN201911209314A CN110794156A CN 110794156 A CN110794156 A CN 110794156A CN 201911209314 A CN201911209314 A CN 201911209314A CN 110794156 A CN110794156 A CN 110794156A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
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Abstract
The invention relates to a measuring chamber for a full-automatic chemiluminescence immunoassay analyzer, which is characterized by being provided with a reaction cup conveying mechanism, wherein the reaction cup conveying mechanism comprises a groove type track positioned on a supporting main body, a reaction cup conveying belt arranged on the track and a driving assembly for driving the reaction cup conveying belt to run along the track; the middle part of the support main body is provided with a detection position, the detection position consists of a cup position to be detected, a detection cup position and a detected cup position which are sequentially arranged along the conveying direction of the reaction cup, a photon counter fixing hole communicated with the groove type track is formed in the side wall of the support main body corresponding to the detection cup position, and the photon counter extends into the fixing hole to count the reaction cup passing through the detection position in the groove type track; compared with the prior art, the invention has the advantages of reasonable structure, simple and convenient assembly, good shading property, capability of effectively prolonging the service life of the lens of the photon counter, improvement on the measurement accuracy and the like.
Description
The technical field is as follows:
the invention relates to the technical field of manufacturing of full-automatic chemiluminescence immunoassay analyzers, in particular to a measuring chamber for a full-automatic chemiluminescence immunoassay analyzer, which has the advantages of reasonable structure, simplicity and convenience in assembly, good light shielding property, capability of effectively prolonging the service life of a lens of a photon counter and improving the measuring accuracy.
Background art:
with the development of new technology, the in vitro diagnosis industry is developed to a large-scale automatic and integrated diagnosis system with high efficiency, high precision and high integration, so as to solve the problem of precise and complex diagnosis; secondly, the method develops to most conventional detection, has irreversible trend, and develops to simple, rapid and accurate POCT (Point-of-care testing) detection, and realizes instant diagnosis and treatment.
Compared with clinical laboratory detection, most POCT detection is mainly semi-quantitative and semi-automatic at present, the automation integration level is not high, and various limitations such as insufficient precision, poor stability, sample environment interference and the like exist in clinical application. For example, chinese patent application No. 201310300162.2 discloses a portable full-automatic chemiluminescence immunoassay system and an analysis method thereof, wherein the system comprises a sample injection part, a liquid path part, a reaction control part, an optical detection part, and a computer control center; wherein the optical detection portion comprises a chemiluminescent measurement device; the chemiluminescence measuring device is matched with the reaction cup structure disclosed in the document, the reaction cups disclosed in the document are provided with a group of multi-hole positions in order to improve the working efficiency, a plurality of samples are respectively added to different hole positions of one reaction cup during detection, then the plurality of samples in one reaction cup are simultaneously pushed into a dark box in the chemiluminescence measuring device for detection through one-time pushing, and although the high pushing quantity and the high speed of the samples can be ensured, the measurement result is inaccurate due to the mutual interference of luminescent substances in the samples in the adjacent hole positions. The chemiluminescence measuring apparatus described in this document is not adjacent to other modules, and includes functions such as liquid pumping and waste liquid suction (in the paragraph of paragraph 60, "a substrate nozzle 197 for injecting a substrate liquid into a cuvette and a second waste liquid needle 198 for sucking away waste liquid") on the inner surface thereof, and therefore, when the excitation liquid is erroneously pumped, the lens of the photon counter is contaminated, and the experimental environment is damaged.
The invention content is as follows:
aiming at the defects and shortcomings in the prior art, the invention provides the measuring chamber for the full-automatic chemiluminescence immunoassay analyzer, which can form a good darkroom environment and isolate adjacent detection samples, thereby avoiding cross influence between the samples, improving reading precision, draining redundant excitation liquid and protecting a lens of a photon counter.
The invention can be achieved by the following measures:
a measuring chamber for a full-automatic chemiluminescence immunoassay analyzer is provided with a supporting main body, an upper cover positioned on the upper surface of the supporting main body, a baffle lifting mechanism positioned below the supporting main body, and a reaction cup conveying mechanism, wherein the reaction cup conveying mechanism comprises a groove type rail positioned on the supporting main body, a reaction cup conveying belt arranged on the rail, and a driving assembly used for driving the reaction cup conveying belt to run along the rail; the middle part of the support main body is provided with a detection position, the detection position consists of a cup position to be detected, a detection cup position and a detected cup position which are sequentially arranged along the conveying direction of the reaction cup, a photon counter fixing hole communicated with the groove type track is formed in the side wall of the support main body corresponding to the detection cup position, and the photon counter extends into the fixing hole to count the reaction cup passing through the detection position in the groove type track; baffle lifting slots communicated with the groove type rails are respectively formed in the front end and the rear end of the support main body bottom plate corresponding to the detection positions, the position between the cup position to be detected and the detection cup position and the position between the detection cup position and the detected cup position; the baffle lifting mechanism is provided with a light blocking bottom plate, the upper surface of the light blocking bottom plate is respectively provided with a detection baffle for separating a detection position, a cup position to be detected, a detection cup position and a detected cup position, and the light blocking bottom plate is also provided with a linear lifting motor for driving the light blocking bottom plate to lift; the detection baffle in the baffle elevating system inserts the both ends of detecting the position respectively along baffle lift slot and waits to detect the cup position, detect between the cup position, the bottom plate that is in the light seals the support subject bottom, and upper cover seals support subject upper portion, and support subject detects the recess formula track part that the position corresponds and forms the darkroom.
The cup body of the reaction cup is in a flat-mouth strip shape, the cross section of the cup body of the reaction cup is rectangular, in the conveying process, the main body part of the cup body of the reaction cup is accommodated in the groove type track on the supporting main body, the cup wall with the larger area of the cup body of the reaction cup faces to the side provided with the photon counter, a limiting hole is formed in a reaction cup conveying belt in the reaction cup conveying mechanism, the reaction cup penetrates through the limiting hole and extends into the groove type track, the cup opening is clamped on the limiting hole, when the reaction cup conveying belt is driven to advance by the driving assembly, the reaction cup advances along the groove type track and sequentially passes through a cup position to be detected, a cup position to be detected and a cup position to be detected, and in order to ensure that the reaction cup advances in the groove type track, the depth of the.
The middle part of the upper cover is provided with an inner concave part corresponding to the running part of the reaction cup conveying mechanism so as to form a gap for facilitating the running of the reaction cup conveying mechanism after being connected with the upper surface of the supporting main body, and the two sides of the inner concave part of the upper cover are provided with bosses/grooves matched with the upper surface of the supporting main body so as to ensure that the upper cover is tightly attached to the supporting main body and avoid the light leakage at the top of the detection position from influencing the dark room effect.
The baffle plate lifting mechanism is provided with a linear lifting motor fixed on the lower side of a linear lifting motor fixing bottom plate, a shaft hole is formed in the linear lifting motor fixing bottom plate, an output shaft of the linear lifting motor extends out through the shaft hole and is connected with a light blocking bottom plate, a fixing side plate connected with a supporting main body is arranged on the upper side of the linear lifting motor fixing bottom plate, a light blocking plate position blocking piece used for limiting the light blocking bottom plate and detecting the downward moving position of a baffle plate is arranged on the fixing side plate, a position sensor used for detecting the light blocking bottom plate and detecting the position of the baffle plate is further arranged on the fixing side plate, the position sensor can be arranged above the light blocking plate position blocking piece, and the position sensor can.
In order to reduce the impact of the detection baffle plate on the light blocking bottom plate during insertion, a spring is arranged between the top disc of the linear lifting motor and the light blocking bottom plate, a shaft sleeve is arranged in the center of the bottom of the light blocking bottom plate, the spring is arranged in the shaft sleeve, an output shaft of the linear lifting motor extends into the shaft sleeve, and the top disc at the front end of the output shaft is in contact with the spring.
The detection baffle plate on the upper surface of the light blocking bottom plate in the baffle plate lifting mechanism comprises a left end baffle plate and a right end baffle plate which are positioned at two ends and used for sealing two ends of a detection position, and also comprises a left detection cup position baffle plate and a right detection cup position baffle plate which are positioned between the left end baffle plate and the right end baffle plate and used for sealing two ends of a detection cup position, wherein the four detection baffle plates are consistent in height, and when the baffle plate lifting mechanism is lifted to the highest point, the detection baffle plates are respectively inserted into two ends of the detection position and the positions of a cup to be detected, the detection cup position and the detected cup position along the baffle plate lifting slot, the detection baffle plate is flush with the surface of the support main body, so that a smooth; in order to further improve the light blocking effect of the detection cup position, the cross sections of the left detection cup position baffle and the right detection cup position baffle are symmetrically arranged in an L shape, the bent part of the left detection cup position baffle/the right detection cup position baffle is close to the photon counter end and is folded outwards towards the position far away from the reaction cup, so that the detection cup position is sealed by the upper cover, the light blocking bottom plate and the detection baffle, an independent small darkroom is formed inside, and photons cannot enter the darkroom.
According to the baffle lifting mechanism, the upper surface of the light-blocking bottom plate is provided with the double annular bosses around the four detection baffles to form the reversed-square cofferdam, and the double annular grooves matched with the double annular bosses are arranged at the corresponding positions of the bottom surface of the supporting main body, so that the attaching degree of the light-blocking bottom plate and the bottom surface of the supporting main body is improved, the light-blocking effect is further improved, and the excitation liquid can be prevented from being mistakenly splashed; furthermore, a liquid storage groove is arranged at a position between the left detection cup position baffle and the right detection cup position baffle on the upper surface of the light blocking bottom plate and used for temporarily storing the mistakenly-beaten excitation liquid or the low-falling waste liquid so as to avoid polluting other parts of the equipment.
A lens glass fixing frame is arranged in a photon counter fixing hole formed in the side wall of the supporting main body, photon counter lens glass is arranged, the photon counter extends into the fixing hole and is fixed through a photon counter fixing plate, an O-shaped ring is arranged between the photon counter and the fixing hole, and the photon counter fixing plate is fixedly connected with a fixing side plate.
The upper cover is also provided with a pre-excitation liquid adding mechanism, an excitation liquid adding mechanism and a waste liquid suction mechanism which are sequentially arranged according to the conveying and advancing direction of the reaction cup, wherein the waste liquid suction mechanism is arranged at the upper part of the detection position, a waste liquid needle guide sleeve and a waste liquid needle guide column which are matched are arranged in the waste liquid suction mechanism, the waste liquid needle guide sleeve and the waste liquid needle guide column are respectively provided with a through hole which is coaxial with a waste liquid needle channel on the top cover, the lower end of the waste liquid needle guide column is inserted into the waste liquid needle through hole on the top cover, the outer diameter of the part of the waste liquid needle guide column positioned at the outer side of the top cover is larger than the width of the waste liquid needle channel on the top cover, the waste liquid needle guide sleeve is covered at the outer side of the waste liquid needle guide column, a screw hole is arranged at the side of the waste liquid needle guide sleeve, the waste liquid needle.
According to the invention, the baffle lifting mechanism driven by the linear lifting motor is arranged, and the light blocking bottom plate and the detection baffle on the light blocking bottom plate are controlled to move up and down according to the acquired data of the position sensor, after the reaction cup to be detected is conveyed into the detection cup position by the reaction cup conveying mechanism, the light blocking bottom plate and the detection baffle are lifted, the light blocking bottom plate and the detection baffle, the support main body and the upper cover form a darkroom environment, three reaction cups in the detection position form independent closed darkrooms respectively, the reaction cup at the detection cup position is read by using the photon counter, and at the moment, two reaction cups adjacent to the reaction cup are isolated outside the independent darkrooms, so that the luminescent substances in the adjacent reaction cups can not generate interference on the detection result; in addition, in order to realize good sealing, the two components are required to be absolutely and tightly matched, and in order to reduce the impact on the linear motor, the top disc of the linear lifting motor is matched with the spring to be contacted with the light blocking bottom plate, so that the flexible sealing of the light blocking bottom plate is realized; the upper limit and the lower limit of the light barrier lifting mechanism are realized by arranging the light barrier position blocking piece; the waste liquid needle is subjected to light-shielding treatment through the matching relationship between the waste liquid needle guide sleeve and the waste liquid needle guide column; by arranging the lens glass, the photon counter is effectively protected.
Compared with the prior art, the invention has the remarkable advantages that a good darkroom environment can be formed, adjacent detection samples are isolated, so that the cross influence among the samples is avoided, the reading precision is improved, redundant excitation liquid can be drained, the lens of the photon counter is protected, and the like.
Description of the drawings:
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a top cross-sectional view of the present invention.
FIG. 3 is a cross-sectional view of a test site according to the present invention.
FIG. 4 is a schematic view showing the connection relationship between the light-blocking bottom plate and the top plate of the linear elevating motor in the barrier elevating mechanism of the present invention.
FIG. 5 is a schematic structural view of the waste liquid needle guide bush and the waste liquid needle guide post according to the present invention.
Fig. 6 is a schematic structural view of a light-blocking bottom plate in the barrier lifting mechanism of the present invention.
FIG. 7 is a schematic diagram showing the structure of a cuvette to be tested according to the present invention.
FIG. 8 is a schematic diagram of the structure of the detection bit of the present invention.
Fig. 9 is a schematic view of the structure of the transfer rack in the present invention.
FIG. 10 is a schematic structural view of the present invention in which a protrusion/recess is formed between the upper lid and the transfer rail body of the cuvette.
Reference numerals: the device comprises a supporting main body 1, an upper cover 2, a groove type track 3, a photon counter 4, a light blocking bottom plate 5, a linear lifting motor 6, a linear lifting motor fixing bottom plate 7, a fixing side plate 8, a light blocking plate position blocking piece 9, a position sensor 10, a position sensor fixing plate 11, a top disc 12, a spring 13, a left end baffle 14, a right end baffle 15, a left detection cup position baffle 16, a right detection cup position baffle 17, a double annular boss 18, a liquid storage groove 19, a lens glass fixing frame 20, a photon counter lens glass 21, a photon counter fixing plate 22, a waste liquid needle guide sleeve 23, a waste liquid needle guide column 24, an O-shaped ring 25, a limiting screw 26, an excitation liquid valve 27, a pre-excitation liquid valve 28, a rack 29, a limiting hole 30, a cup position 31 to be detected, a detection cup position 32, a detected cup position 33 and a waste liquid.
The specific implementation mode is as follows:
the invention will be further described with reference to the accompanying drawings.
As shown in the attached figure 1, the invention provides a measuring chamber for a full-automatic chemiluminescence immunoassay analyzer, which is provided with a supporting main body 1, an upper cover 2 positioned on the upper surface of the supporting main body 1 and a baffle lifting mechanism positioned below the supporting main body 1, and is characterized by also being provided with a reaction cup conveying mechanism, wherein the reaction cup conveying mechanism comprises a groove type track 3 positioned on the supporting main body 1, a reaction cup conveying rack arranged on the track 3 and a driving component for driving the reaction cup conveying rack to run along the track; a detection position is arranged in the middle of the supporting main body 1, the detection position consists of a cup position to be detected, a detection cup position and a detected cup position which are sequentially arranged along the conveying direction of the reaction cup, a photon counter fixing hole communicated with the groove type track is formed in the side wall of the supporting main body 1 corresponding to the detection cup position, and the photon counter 4 extends into the fixing hole to count the reaction cup passing through the detection position in the groove type track 3; baffle lifting slots communicated with the groove type track 3 are respectively formed in the front end and the rear end of the bottom plate of the supporting main body 1, which correspond to the detection positions, the positions between the cup positions to be detected and the detection cup positions and the positions between the detection cup positions and the detected cup positions; as shown in fig. 1 and fig. 6, the barrier lifting mechanism is provided with a light-blocking bottom plate 5, the upper surface of the light-blocking bottom plate 5 is respectively provided with a detection barrier for separating a detection position and a cup position to be detected, a detection cup position and a detected cup position, and is further provided with a linear lifting motor 6 for driving the light-blocking bottom plate 5 to lift; the detection baffle in the baffle elevating system inserts the both ends of detecting the position respectively along baffle lift slot and waits to detect the cup position, detect between the cup position, the bottom plate 5 that is in the light seals the support subject 1 bottom, and upper cover 2 seals support subject 1 upper portion, and support subject 1 detects the recess formula track 3 part that the position corresponds and forms the darkroom.
As shown in the attached figures 8 and 9, the shape of the reaction cup of the invention can adopt any one of the attached figures 7, preferably, the cup body of the reaction cup is in a flat-mouth strip shape, furthermore, the cross section of the cup body of the reaction cup is in a rectangular shape, in the conveying process, the main body part of the cup body of the reaction cup is accommodated in the groove type track 3 on the support main body 1, the cup wall with larger area of the reaction cup body faces to the side provided with the photon counter 4, a reaction cup limiting hole 30 is arranged on a reaction cup transmission rack 29 in the reaction cup transmission mechanism, the reaction cup passes through the limiting hole 30 and extends into the groove type track 3, the cup mouth is blocked on the limiting hole, when the reaction cup conveyor belt is driven by the driving component to advance, the reaction cup advances along the groove-type track 3 and passes through the cup position to be detected 31, the cup position to be detected 32 and the cup position to be detected 33 in sequence, in order to ensure that the reaction cup advances in the groove-type track 3, the depth of the groove-type track 3 is more than or equal to the height of the cup body of the reaction cup.
As shown in the attached figure 10, the center of the upper cover 2 is provided with an inner concave part corresponding to the running part of the reaction cup conveying mechanism, so that a gap convenient for the running of the reaction cup conveying mechanism is formed after the reaction cup conveying mechanism is connected with the upper surface of the supporting main body 1, and bosses/grooves matched with the upper surface of the supporting main body 1 are arranged on two sides of the inner concave part of the upper cover 2, so that the upper cover 2 is ensured to be tightly attached to the supporting main body 1, and the influence of light leakage at the top of a detection position on the darkroom effect is.
As shown in fig. 1, the baffle plate lifting mechanism of the invention is provided with a linear lifting motor 6 fixed on the lower side of a linear lifting motor fixing bottom plate 7, a shaft hole is arranged on the linear lifting motor fixing bottom plate 7, an output shaft of the linear lifting motor 6 extends out through the shaft hole and is connected with a light blocking bottom plate 5, a fixing side plate 8 connected with a supporting body 1 is arranged on the upper side of the linear lifting motor fixing bottom plate 7, a light blocking plate position blocking piece 9 used for limiting the light blocking bottom plate and detecting the downward moving position of the baffle plate is arranged on the fixing side plate 8, and a position sensor 10 used for detecting the light blocking bottom plate 5 and detecting the position of the baffle plate is further arranged, the position sensor 10 can be arranged above the light blocking plate position blocking piece 9 and can be connected and fixed through.
As shown in fig. 1 and fig. 4, in order to reduce the impact of the insertion of the detection baffle on the light-blocking bottom plate 5, a spring 13 is arranged between the top plate 12 of the linear lifting motor 6 and the light-blocking bottom plate 5, wherein a shaft sleeve is arranged at the center of the bottom of the light-blocking bottom plate 5, the spring 13 is arranged in the shaft sleeve, the output shaft of the linear lifting motor 6 extends into the shaft sleeve, and the top plate 12 at the front end of the output shaft is in contact with the spring 13.
As shown in fig. 6, the detection baffle on the upper surface of the light-blocking bottom plate 5 in the baffle lifting mechanism of the invention comprises a left end baffle 14 and a right end baffle 15 which are positioned at two ends and used for sealing two ends of a detection position, and also comprises a left detection cup position baffle 16 and a right detection cup position baffle 17 which are positioned between the left end baffle 14 and the right end baffle 15 and used for sealing two ends of a detection cup position, wherein the four detection baffles have consistent heights, and when the baffle lifting mechanism is lifted to the highest point, the detection baffles are respectively inserted into two ends of the detection position and a cup position to be detected, the detection cup position and a detected cup position along the baffle lifting slot, the detection baffles are flush with the upper surface of the support main body 1, so as to form a smooth working surface which is beneficial to the operation; in order to further improve the light blocking effect on the detection cup position, the cross sections of the left detection cup position baffle 16 and the right detection cup position baffle 17 are symmetrically arranged in an L shape, the bent part of the left detection cup position baffle 16/the right detection cup position baffle 17 is close to the photon counter end and is folded outwards towards the position far away from the reaction cup, so that the detection cup position is sealed by the upper cover 2, the light blocking bottom plate 5 and the detection baffle, an independent small dark room is formed inside, and the situation that photons cannot enter the dark room is guaranteed.
As shown in fig. 6, the upper surface of the light-blocking bottom plate in the baffle lifting mechanism of the invention is provided with double annular bosses 18 around four detection baffles to form a rectangular-shaped cofferdam, and at the same time, double annular grooves matched with the double annular bosses are arranged at corresponding positions on the bottom surface of the supporting main body to improve the fitting degree of the light-blocking bottom plate and the bottom surface of the supporting main body, further improve the light-blocking effect and prevent excitation liquid from mistakenly overflowing; further, a liquid storage groove 19 is formed in the position between the left detection cup position baffle and the right detection cup position baffle on the upper surface of the light blocking bottom plate 5 and used for temporarily storing the mistakenly-beaten excitation liquid or the fallen waste liquid to avoid polluting other parts of the equipment.
As shown in fig. 1 and fig. 2, a lens glass fixing frame 20 and a photon counter lens glass 21 are arranged in a photon counter fixing hole formed in the side wall of the support main body 1, the photon counter 4 extends into the fixing hole and is fixed by a photon counter fixing plate 22, an O-ring 25 is arranged between the photon counter 4 and the fixing hole, and the photon counter fixing plate 22 is fixedly connected with the fixing side plate 8.
As shown in the attached figure 5, the upper cover 2 of the invention is also provided with a pre-excitation liquid adding mechanism, an excitation liquid adding mechanism and a waste liquid absorbing mechanism which are arranged in sequence according to the conveying advancing direction of the reaction cup, wherein the waste liquid suction mechanism is arranged at the upper part of the detection position, a waste liquid needle guide sleeve 23 and a waste liquid needle guide column 24 which are matched with each other are arranged in the waste liquid suction mechanism, wherein the waste liquid needle guide sleeve 23 and the waste liquid needle guide column 24 are respectively provided with a through hole coaxial with a waste liquid needle channel on the top cover 2, the lower end of the waste liquid needle guide column 24 is inserted in the waste liquid needle through hole on the top cover 2, the outer diameter of the part of the waste liquid needle guide column 24 positioned at the outer side of the top cover is larger than the width of the waste liquid needle channel on the top cover, the waste liquid needle guide sleeve 23 covers the outer side of the waste liquid needle guide column 24, through seting up the screw hole in waste liquid needle guide sleeve 23 side, lock into the screw hole through stop screw 26 and accomplish waste liquid needle guide sleeve 23's location, this kind of structure carries out the light-resistant processing to the waste liquid needle that stretches into and detect the position.
According to the invention, the baffle lifting mechanism driven by the linear lifting motor is arranged, and the light blocking bottom plate and the detection baffle on the light blocking bottom plate are controlled to move up and down according to the acquired data of the position sensor, after the reaction cup to be detected is conveyed into the detection cup position by the reaction cup conveying mechanism, the light blocking bottom plate and the detection baffle are lifted, the light blocking bottom plate and the detection baffle, the support main body and the upper cover form a darkroom environment, three reaction cups in the detection position form independent closed darkrooms respectively, the reaction cup at the detection cup position is read by using the photon counter, and at the moment, two reaction cups adjacent to the reaction cup are isolated outside the independent darkrooms, so that the luminescent substances in the adjacent reaction cups can not generate interference on the detection result; in addition, in order to reduce the impact on the linear motor, the top disc of the linear lifting motor is contacted with the light blocking bottom plate by matching with a spring, so that the flexible sealing of the light blocking bottom plate is realized; the upper limit and the lower limit of the light barrier lifting mechanism are realized by arranging the light barrier position blocking piece; the waste liquid needle is subjected to light-shielding treatment through the matching relationship between the waste liquid needle guide sleeve and the waste liquid needle guide column; by arranging the lens glass, the photon counter is effectively protected.
Compared with the prior art, the invention has the remarkable advantages that a good darkroom environment can be formed, adjacent detection samples are isolated, so that the cross influence among the samples is avoided, the reading precision is improved, redundant excitation liquid can be drained, the lens of the photon counter is protected, and the like.
Claims (10)
1. A measuring chamber for a full-automatic chemiluminescence immunoassay analyzer is provided with a supporting main body, an upper cover positioned on the upper surface of the supporting main body, a baffle lifting mechanism positioned below the supporting main body, and a reaction cup conveying mechanism, wherein the reaction cup conveying mechanism comprises a groove type rail positioned on the supporting main body, a reaction cup conveying belt arranged on the rail, and a driving assembly used for driving the reaction cup conveying belt to run along the rail; the middle part of the support main body is provided with a detection position, the detection position consists of a cup position to be detected, a detection cup position and a detected cup position which are sequentially arranged along the conveying direction of the reaction cup, a photon counter fixing hole communicated with the groove type track is formed in the side wall of the support main body corresponding to the detection cup position, and the photon counter extends into the fixing hole to count the reaction cup passing through the detection position in the groove type track; baffle lifting slots communicated with the groove type rails are respectively formed in the front end and the rear end of the support main body bottom plate corresponding to the detection positions, the position between the cup position to be detected and the detection cup position and the position between the detection cup position and the detected cup position; the baffle lifting mechanism is provided with a light blocking bottom plate, the upper surface of the light blocking bottom plate is respectively provided with a detection baffle for separating a detection position, a cup position to be detected, a detection cup position and a detected cup position, and the light blocking bottom plate is also provided with a linear lifting motor for driving the light blocking bottom plate to lift; the detection baffle in the baffle elevating system inserts the both ends of detecting the position respectively along baffle lift slot and waits to detect the cup position, detect between the cup position, the bottom plate that is in the light seals the support subject bottom, and upper cover seals support subject upper portion, and support subject detects the recess formula track part that the position corresponds and forms the darkroom.
2. The measuring chamber of claim 1, wherein when the conveyor belt of the reaction cup is driven by the driving assembly to advance, the reaction cup advances along the grooved track and passes through the cup position to be detected, the detection cup position and the detected cup position in sequence, and the depth of the grooved track is greater than or equal to the height of the cup body of the reaction cup.
3. The measuring chamber of claim 1, wherein the central portion of the upper cover has an inner recess corresponding to the operation portion of the cuvette conveying mechanism, so that a gap for the operation of the cuvette conveying mechanism is formed after the upper cover is connected to the upper surface of the support body, and the inner recess of the upper cover has a protrusion/recess on both sides thereof for engaging with the upper surface of the support body.
4. The measuring chamber of claim 1, wherein the barrier elevating mechanism comprises a linear elevating motor fixed on the lower side of the linear elevating motor fixing base plate, the linear elevating motor fixing base plate is provided with a shaft hole, the output shaft of the linear elevating motor extends through the shaft hole and is connected with the light blocking base plate, the upper side of the linear elevating motor fixing base plate is provided with a fixing side plate connected with the support body, the fixing side plate is provided with a light blocking plate position blocking piece for limiting the light blocking base plate and detecting the downward movement position of the barrier, and a position sensor for detecting the light blocking base plate and detecting the position of the barrier is provided.
5. The measuring chamber of claim 1, wherein a spring is disposed between the top plate and the light-blocking bottom plate of the linear lift motor, wherein a shaft sleeve is disposed at the bottom center of the light-blocking bottom plate, a spring is disposed in the shaft sleeve, the output shaft of the linear lift motor extends into the shaft sleeve, and the top plate at the front end of the output shaft contacts with the spring.
6. The measuring chamber of claim 1, wherein the detecting barrier on the upper surface of the light-blocking bottom plate of the barrier lifting mechanism comprises a left end barrier and a right end barrier at two ends for closing two ends of the detecting position, and further comprises a left detecting cup barrier and a right detecting cup barrier between the left end barrier and the right end barrier for closing two ends of the detecting cup position, the four detecting barriers have the same height, and when the barrier lifting mechanism is lifted to the highest point, the detecting barriers are respectively inserted into two ends of the detecting position along the barrier lifting slot, and the detecting cup position, the detecting cup position and the detected cup position are flush with the surface of the supporting body, so as to form a flat working surface beneficial to the operation of the reaction cup conveying mechanism.
7. The measuring chamber of claim 6, wherein the cross-section of the left and right detection cup position baffles is symmetrically L-shaped, and the bent portion of the left/right detection cup position baffles is close to the photon counter end and is folded outward away from the reaction cup.
8. The measuring chamber of claim 1, wherein the baffle elevating mechanism comprises a double ring-shaped protruding platform on the upper surface of the light-blocking bottom plate around the four detection baffles to form a rectangular cofferdam, and a double ring-shaped groove matching with the double ring-shaped protruding platform is disposed at a position corresponding to the bottom surface of the supporting body to improve the adhesion between the light-blocking bottom plate and the bottom surface of the supporting body, and a liquid storage tank is disposed at a position between the left detection cup position baffle and the right detection cup position baffle on the upper surface of the light-blocking bottom plate.
9. The measuring chamber of claim 1, wherein a lens holder is disposed in a photon counter holder hole formed in a side wall of the support body, and a lens of the photon counter is disposed, the photon counter is inserted into the holder hole and fixed by a photon counter holder plate, an O-ring is disposed between the photon counter and the holder hole, and the photon counter holder plate is connected and fixed to the fixing side plate.
10. The measuring chamber for a full-automatic chemiluminescence immunoassay analyzer according to claim 1, it is characterized in that the upper cover of the invention is also provided with a pre-excitation liquid adding mechanism, an excitation liquid adding mechanism and a waste liquid absorbing mechanism which are arranged in sequence according to the conveying advancing direction of the reaction cup, wherein the waste liquid suction mechanism is arranged at the upper part of the detection position, a waste liquid needle guide sleeve and a waste liquid needle guide column which are matched with each other are arranged in the waste liquid suction mechanism, wherein the waste liquid needle guide sleeve and the waste liquid needle guide column are respectively provided with a through hole coaxial with the waste liquid needle channel on the top cover, the lower end of the waste liquid needle guide column is inserted in the waste liquid needle through hole on the top cover, the outer diameter of the part of the waste liquid needle guide column positioned at the outer side of the top cover is larger than the width of the waste liquid needle channel on the top cover, the waste liquid needle guide sleeve covers the outer side of the waste liquid needle guide column, the side surface of the waste liquid needle guide sleeve is provided with a screw hole, and the waste liquid needle guide sleeve is locked into the screw hole through a limit screw to complete the positioning of the waste liquid needle guide sleeve.
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