CN110772664A - A kind of preparation method of surface intelligent coating of orthopaedic temporary implant based on natural polysaccharide and product thereof - Google Patents

Abstract

The invention discloses a preparation method of a natural polysaccharide-based orthopaedic temporary implant surface intelligent coating. The steps are: 1) reacting a first polysaccharide with sodium periodate to obtain a degree of hydroformylation of 55% to 65%. 2) Dissolving the reinforcing polysaccharide in hot water to obtain solution a, dissolving the antibiotic in a sodium carbonate solution with pH=9 to 10 to obtain solution b, adding solution b to solution a and mixing evenly, Add the hydroformyl polysaccharide described in step 1) to prepare a film-forming liquid; 3) put a temporary orthopaedic implant in the reactor, add the film-forming liquid described in step 2), and slowly volatilize at 30-60 °C, The surface of the orthopaedic temporary implant is evenly wrapped with a composite film; 4) the material prepared in step 3) is put into a solution of adding a cross-linking agent, soaked for 5-20 minutes, taken out, washed with water, and dried to obtain a composite film modified with a composite film. Orthopedic temporary implants. The product of the invention has excellent antibacterial effect.

Description

Preparation of a Surface Smart Coating of Natural Polysaccharide-Based Orthopedic Temporary Implants method and product

technical field

The invention belongs to the field of medical materials, and relates to a preparation method and a product of a surface intelligent coating of a natural polysaccharide-based orthopaedic temporary implant.

Background technique

Implant-related infections often occur clinically in orthopaedics, mainly due to the two major problems of local bacterial proliferation and bacteremia. Orthopedic implant infection has the characteristics of high incidence, recurrence and delay, which often lead to treatment failure and even endanger the health of patients. To address the infection problem of orthopaedic implants, many efforts have been made in surface functionalization. For example, build a cationic coating on the surface for contact sterilization. Among the bactericidal cationic polymers reported in the literature, polycations derived from quaternary ammonium compounds (QACs), polyethyleneimine derivatives, chitosan derivatives, etc. disrupt the integrity of bacterial cell membranes through membrane lysis mechanisms, allowing cell contents Leak and show strong antibacterial activity. In addition, a bactericide can be loaded on the surface for release-type sterilization. Biocides including silver nanoparticles (AgNPs), antibiotics, and nitrogen oxides are preloaded or embedded and then slowly released into the nearby environment to kill bacteria. It is also reported that photothermal sterilization is carried out on the surface modified photosensitizer, and the photothermal agent materials such as platinum and gold are irradiated with near-infrared light to generate excessive heat, and bacteria are killed by thermal ablation.

Chinese Patent Publication No. CN106512083A discloses a preparation method of medical titanium alloy. The patent uses double emulsion method, hydrothermal treatment, etc. to make PLGA drug-loaded nanoparticles connect to the surface of titanium base, and the prepared product has good biological activity and antibacterial properties. However, this method has complicated steps and involves strong acid and strong base in the experimental process, which is dangerous and lacks high efficiency and practicability.

Chinese Patent Publication No. CN102758202B discloses a preparation method of an antibacterial coating on the surface of medical titanium and titanium alloys. This patent combines the preparation of nano-precoating, silver-loading treatment and micro-arc oxidation technology to achieve a large amount of silver fixation and long-term slow release on the surface of medical titanium and titanium alloys, which can significantly improve the antibacterial properties of titanium and titanium alloys. And the antibacterial effect can be maintained for a long time. However, the antibacterial agent silver in this method has certain biological toxicity, which limits the application of this method.

Chinese Patent Publication No. CN106867042A discloses a method for preparing nanocellulose/chitosan/polyvinyl alcohol composite film by casting method and its application method in biological antibacterial film. The patent uses polyvinyl alcohol as the matrix polymer, nanocellulose as the reinforcing agent, and chitosan as the natural antibacterial agent to complete the preparation process of the composite film product by the casting method. The composite membrane prepared by this method is easy to operate, has excellent mechanical properties and good optical properties, but the firm combination of chitosan can easily lead to bacterial drug resistance due to the long-term existence of chitosan.

Chinese Patent Publication No. CN103191467A discloses a preparation method of an antibacterial coating for slow-release of various cell growth factors on a medical metal surface. In this method, the antibiotics with amino groups are grafted on graphene oxide by covalent bonding of amide bonds, and the nanoparticles wrapped with various cell growth factors are fixed between the graphene oxide layers by layer-by-layer self-assembly method. As a result, a coating simultaneously loaded with a variety of cell growth factors and antibiotics is obtained on the medical metal surface. The coating prepared by the method can independently control the slow and orderly release of various cell growth factors, and can release antibiotics in the whole process of the slow release of cell growth factors. However, this method cannot control the adaptive release of antibiotics, the graphene oxide used is potentially toxic, and the layer-by-layer self-assembly lacks a certain high efficiency and practicality.

As mentioned above, there are still some problems in surface functionalization. Antibacterial agents have long-term induced bacterial resistance, bacteria are easily colonized on the surface, leading to the failure of antibacterial agents, poor compatibility of implant surfaces, and certain toxicity of selected materials. In order to solve the above problems, the present invention hopes to develop an intelligent coating with simple preparation process, good biocompatibility and self-adaptive release antibacterial properties. Smart coating refers to the surface modification of orthopaedic temporary implants to make them responsive to bacteria and sterilize by adaptively releasing antibacterial substances to achieve the purpose of anti-infection, thereby improving the success rate of implantation operations. The smart coating developed by the present invention provides the reaction site with firm natural polysaccharide on the surface, so that the implant has good biocompatibility. Antibiotics and polysaccharide sites are connected by Schiff base bonds that respond to the acidic microenvironment of bacteria, so that antibiotics can be adaptively released for sterilization, so as to achieve rapid, accurate and efficient killing of bacteria, while avoiding bacterial resistance to a great extent. production of medicinal properties.

SUMMARY OF THE INVENTION

In view of this, the purpose of the present invention is to not only make the implant have good biocompatibility, but also have excellent anti-infection performance, and provide a kind of preparation of the surface intelligent coating of the orthopaedic temporary implant based on natural polysaccharide method.

The present invention specifically provides the following technical solutions:

1, a kind of preparation method of the surface intelligent coating of the orthopaedic temporary implant based on natural polysaccharide, the steps are:

1) reacting the first polysaccharide with sodium periodate to obtain an hydroformylation polysaccharide with a degree of hydroformylation of 55% to 65%;

2) Dissolving the reinforcing polysaccharide in hot water to obtain solution a, dissolving the antibiotic in a sodium carbonate solution with pH=9～10 to obtain solution b, adding solution b to solution a and mixing evenly, adding the step 1) described The hydroformylation polysaccharide was obtained to obtain a film-forming liquid;

3) Putting the temporary orthopaedic implant into the reactor, adding the film-forming liquid described in step 2), and slowly volatilizing at 30-60°C, and evenly wrapping the surface of the temporary orthopaedic implant with a composite film;

4) Put the material prepared in step 3) into the solution with added cross-linking agent, soak for 5-20 minutes, take out, wash with water, and dry to obtain a temporary orthopaedic implant modified with a composite membrane.

Further, the first polysaccharide described in step 1) is cellulose, starch, sodium alginate, sodium hyaluronate, dextran, pullulan, agarose or laminose, and the reinforcing described in step 2) The polysaccharide is gelatin, xanthan, pectin or acacia.

Further, the first polysaccharide described in step 1) is sodium alginate, and the reinforcing polysaccharide described in step 2) is gelatin.

Further, in terms of parts by mass, the first polysaccharide described in step 1) is 1-15 parts, the sodium periodate is 0.1-5 parts, the hydroformyl polysaccharide described in step 2) is 1-10 parts, and the supplement 5-50 parts of strong polysaccharides, 0.5-5 parts of sodium carbonate, 0.1-3 parts of antibiotics, and 1-3 parts of the cross-linking agent described in step 3), wherein the parts of reinforcing polysaccharides should be higher than that of aldehydes Polysaccharide fractions.

Further, the hydroformylation polysaccharide described in step 2) is 1-5 parts, and the reinforcing polysaccharide is 2-10 parts.

Further, the reaction temperature of the reaction in step 1) is 25°C to 37°C, and the reaction time is 20h to 30h.

Further, the temperature of the hot water in step 2) is 60-100°C.

Further, the volatilization time in step 3) is 7h-10h.

Further, the cross-linking agent described in step 4) is genipin, glutaraldehyde or formaldehyde.

2. A material prepared according to the above-mentioned preparation method for a surface intelligent coating of a natural polysaccharide-based orthopaedic temporary implant.

The beneficial effects of the present invention are:

(1) The present invention uses two different polysaccharides, one is a reinforcing polysaccharide and the other is an hydroformylation polysaccharide, which is slowly volatilized by blending at 30-60 ° C, so that the molecular chain gradually becomes orderly and regular from a random state, A network system is formed on the surface of the material through hydrogen bonding, etc., and a coating modification visible to the naked eye is realized on the surface through a one-step method. The preparation method is simple to operate, the experimental process is green and environmentally friendly, the results are controllable, and the preparation cost is low. In the present invention, the ratio of reinforcing polysaccharide is higher than that of hydroformylation polysaccharide when regulating the proportion of polysaccharide, because when the ratio of hydroformylation polysaccharide is too high, the degree of cross-linking between the two polysaccharides will increase, the molecular chain will be more tightly entangled, and the molecular chain will be more tightly entangled. The moving speed slows down, and the molecular chain does not have time to rearrange when the water volatilizes, resulting in the rupture of the surface film, while the gelatin still maintains the characteristics of protein elasticity. When the proportion of the molecular chain is high, the molecular chain has a certain elasticity. The surface is uniformly formed into a film.

(2) The present invention adopts the Schiff base bond formed by the aldehyde group on the hydroformylation polysaccharide and the amino group of the antibiotic during blending and volatilization to realize the self-adaptive release of the antibiotic. Compared with the systemic administration method, a higher concentration can be locally formed without entering the circulation. The system has no toxic and side effects on important organs of the body, realizes intelligent, fast, efficient and accurate sterilization, and effectively reduces the generation of bacterial drug resistance.

(3) The use of the cross-linking agent of the present invention enables the material to use gelatin to enhance the film-forming effect while avoiding the problem that its structure is easily disintegrated, so that the surface coating can achieve a long-term stable antibacterial effect. Since gelatin is a temperature-sensitive material, its structure will dissolve in the human body at 37°C. In vitro simulation experiments show that the surface coating can be completely dissolved by soaking in normal saline at 37°C for 1 to 6 hours, which does not meet the long-term stability assumption. Step 4) The post-treatment of the film with the cross-linking agent can make it have good stability. After 7 days of immersion experiment at 37 °C in vitro, no structural dissolution of the surface coating occurred after 7 days of immersion. Good antibacterial effect.

(4) The present invention uses natural polysaccharides from a wide range to provide reaction sites, so that the modified implant has good biocompatibility, good antifouling effect, is safe and non-toxic, and can be directly used for medical purposes.

(5) The invention has excellent antibacterial performance, has long-term stable antibacterial effect, and can reduce infection in orthopedic surgery to a certain extent.

Description of drawings

In order to make the purpose, technical solutions and beneficial effects of the present invention clearer, the present invention provides the following accompanying drawings:

FIG. 1 is a comparison diagram of the effect before and after the surface modification of the implant material according to Example 1. FIG.

Fig. 2 shows the comparison before and after hydroformylation of polysaccharide according to step (1) of Example 1 and the characterization of Schiff's reagent.

3 is a graph showing the antibacterial effect of the smart coating on the surface of the natural polysaccharide-based orthopaedic temporary implant obtained according to Examples 2, 4, and 5.

FIG. 4 is a graph showing the effect of film formation on the implant surface of Comparative Example 1 and Comparative Example 2. FIG.

Detailed ways

The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

Example 1

(1) 1 g of sodium alginate was reacted with 0.432 g of sodium periodate at 25°C for 24 hours, then dialyzed with deionized water for 3 days, and freeze-dried to obtain the required hydroformylation sodium alginate with a degree of hydroformylation of 35%. ;

(2) Add 150 mg of gelatin to the vial, add 3 mL of water and heat to dissolve at 60°C to obtain solution 1, weigh 20 mg of antibiotics and dissolve in 2 mL of pH=10 sodium carbonate solution to obtain solution 2, add solution 2 to solution 1 Mix evenly, and after mixing for a period of time, add 50 mg of hydroformyl sodium alginate with a degree of hydroformylation of 35% to prepare a film-forming liquid;

(3) Put the orthopaedic temporary implant in the reactor, add the film-forming solution, the whole system is slowly volatilized at 40°C for 7 hours, and the surface of the orthopaedic temporary implant is evenly wrapped with a composite film.

(4) A glutaraldehyde solution with a mass fraction of 2.5% was prepared with water, the material prepared in (3) was soaked in the glutaraldehyde solution for 30 minutes, taken out, washed with water, and dried to obtain a temporary orthopaedic implant with a modified composite membrane. into the body.

Figure 1 is an effect diagram of comparing the orthopaedic temporary implant modified with a composite membrane and the original implant according to the steps of Example 1. As can be seen from Figure 1, by the preparation method of Example 1, the implant is The surface is evenly decorated with a layer of film.

Figure 2(a) is an effect diagram of comparing the polysaccharide with the original polysaccharide after hydroformylation according to the step (1) of Example 1. Before hydroformylation, the polysaccharide is powdery and slightly yellow, and after hydroformylation, it is white Cotton flocculent, and the color reaction of polysaccharide is carried out with Schiff's reagent - the specific operation steps are to add 1 mL of Schiff's reagent to the test tube, put a certain amount of polysaccharide before and after hydroformylation into it, and observe it after placing it for a period of time, as shown in Figure 2 ( b) It can be seen that the unformaldehyde polysaccharide does not react with the Schiff reagent. After the hydroformylation of the polysaccharide, due to the reaction between the aldehyde group and the Schiff reagent, the solution turns purple, which proves that the polysaccharide is successfully hydroformylated.

Example 2

(1) 1 g of sodium alginate was reacted with 1.03 g of sodium periodate at 25°C for 24 hours, then dialyzed with deionized water for 3 days, and freeze-dried to obtain the required hydroformylation sodium alginate with a degree of hydroformylation of 56%. ;

(2) Add 150 mg of gelatin to the vial, add 3 mL of water and heat at 60°C to dissolve to obtain solution 1, weigh 20 mg of antibiotics and dissolve in 2 mL of sodium carbonate solution with pH=10 to obtain solution 2, add solution 2 to solution 1 and mix Evenly, after mixing for a period of time, add 50 mg of hydroformyl sodium alginate with a degree of hydroformylation of 56% to obtain a film-forming liquid;

(3) Put the orthopaedic temporary implant in the reactor, add the film-forming solution, the whole system is slowly volatilized at 40°C for 7 hours, and the surface of the orthopaedic temporary implant is evenly wrapped with a composite film.

(4) A glutaraldehyde solution with a mass fraction of 2.5% was prepared with water, the material prepared in (3) was soaked in the glutaraldehyde solution for 30 minutes, taken out, washed with water, and dried to obtain a temporary orthopaedic implant with a modified composite membrane. into the body.

Example 3

(1) 1 g of sodium alginate was reacted with 1.5 g of sodium periodate at 25°C for 24 hours, then dialyzed with deionized water for 3 days, and freeze-dried to obtain the required hydroformylation sodium alginate with a degree of hydroformylation of 81.3%. ;

(2) Add 150 mg of gelatin to the vial, add 3 mL of water and heat at 60°C to dissolve to obtain solution 1, weigh 20 mg of antibiotics and dissolve in 2 mL of sodium carbonate solution with pH=10 to obtain solution 2, add solution 2 to solution 1 and mix Evenly, after mixing for a period of time, add 50 mg of hydroformyl sodium alginate with a degree of hydroformylation of 81.3% to obtain a film-forming liquid;

(3) Put the orthopaedic temporary implant in the reactor, add the film-forming solution, the whole system is slowly volatilized at 40°C for 7 hours, and the surface of the orthopaedic temporary implant is evenly wrapped with a composite film.

(4) A glutaraldehyde solution with a mass fraction of 2.5% was prepared with water, the material prepared in (3) was soaked in the glutaraldehyde solution for 30 minutes, taken out, washed with water, and dried to obtain a temporary orthopaedic implant with a modified composite membrane. into the body.

Example 4

(1) 1 g of sodium hyaluronate was reacted with 0.532 g of sodium periodate at 25°C for 24 h, then dialyzed with deionized water for 3 days, and freeze-dried to obtain the desired hydroformylation degree of 45% of hydroformyl hyaluronate sodium;

(2) Add 100 mg of pectin to the vial, add 3 mL of water and heat at 60°C to dissolve to obtain solution 1, weigh 20 mg of antibiotics and dissolve in 2 mL of sodium carbonate solution with pH=10 to obtain solution 2, add solution 2 to solution 1 Mix evenly, and after mixing for a period of time, add 50 mg of hydroformyl sodium hyaluronate with a degree of hydroformylation of 45% to prepare a film-forming liquid;

(3) Put the orthopaedic temporary implant in the reactor, add the film-forming solution, the whole system is slowly volatilized at 40°C for 7 hours, and the surface of the orthopaedic temporary implant is evenly wrapped with a composite film.

(4) A glutaraldehyde solution with a mass fraction of 2.5% was prepared with water, the material prepared in (3) was soaked in the glutaraldehyde solution for 30 minutes, taken out, washed with water, and dried to obtain a temporary orthopaedic implant with a modified composite membrane. into the body.

Example 5

(1) 1 g of dextran was reacted with 0.924 g of sodium periodate at 25°C for 24 h, then dialyzed with deionized water for 3 days, and freeze-dried to obtain the desired hydroformylation degree of hyaluronic acid of 55.3%. sodium;

(2) Add 150 mg of xanthan gum to the vial, add 3 mL of water and heat at 60°C to dissolve to obtain solution 1, weigh 20 mg of antibiotics and dissolve in 2 mL of sodium carbonate solution with pH=10 to obtain solution 2, add solution 2 to solution 1 Mix well, and after mixing for a period of time, add 50 mg of hydroformyl dextran with a degree of hydroformylation of 55.3% to prepare a film-forming liquid;

(3) Put the orthopaedic temporary implant in the reactor, add the film-forming solution, the whole system is slowly volatilized at 40°C for 7 hours, and the surface of the orthopaedic temporary implant is evenly wrapped with a composite film.

(4) A glutaraldehyde solution with a mass fraction of 2.5% was prepared with water, the material prepared in (3) was soaked in the glutaraldehyde solution for 30 minutes, taken out, washed with water, and dried to obtain a temporary orthopaedic implant with a modified composite membrane. into the body.

Comparative Example 1

(1) 1 g of sodium alginate was reacted with 1.03 g of sodium periodate at 25°C for 24 hours, then dialyzed with deionized water for 3 days, and freeze-dried to obtain the required hydroformylation sodium alginate with a degree of hydroformylation of 56%. ;

(2) Add 100 mg of gelatin to the vial, add 3 mL of water and heat at 60°C to dissolve to obtain solution 1, weigh 20 mg of antibiotics and dissolve in 2 mL of sodium carbonate solution with pH=10 to obtain solution 2, add solution 2 to solution 1 and mix Evenly, after mixing for a period of time, add 150 mg of hydroformyl sodium alginate with a degree of hydroformylation of 56% to prepare a film-forming liquid;

(3) Put the orthopaedic temporary implant in the reactor, add the film-forming solution, the whole system is slowly volatilized at 40°C for 7 hours, and the surface of the orthopaedic temporary implant is evenly wrapped with a composite film.

(4) A glutaraldehyde solution with a mass fraction of 2.5% was prepared with water, the material prepared in (3) was soaked in the glutaraldehyde solution for 30 minutes, taken out, washed with water, and dried to obtain a temporary orthopaedic implant with a modified composite membrane. into the body.

Comparative Example 2

(1) 1 g of sodium alginate was reacted with 1.03 g of sodium periodate at 25°C for 24 hours, then dialyzed with deionized water for 3 days, and freeze-dried to obtain the required hydroformylation sodium alginate with a degree of hydroformylation of 56%. ;

(2) Add 100 mg of gelatin to the vial, add 3 mL of water and heat at 60°C to dissolve to obtain solution 1, weigh 20 mg of antibiotics and dissolve in 2 mL of sodium carbonate solution with pH=10 to obtain solution 2, add solution 2 to solution 1 and mix Evenly, after mixing for a period of time, add 100 mg of hydroformyl sodium alginate with a degree of hydroformylation of 56% to prepare a film-forming liquid;

(3) Put the orthopaedic temporary implant in the reactor, add the film-forming solution, the whole system is slowly volatilized at 40°C for 7 hours, and the surface of the orthopaedic temporary implant is evenly wrapped with a composite film.

(4) A glutaraldehyde solution with a mass fraction of 2.5% was prepared with water, the material prepared in (3) was soaked in the glutaraldehyde solution for 30 minutes, taken out, washed with water, and dried to obtain a temporary orthopaedic implant with a modified composite membrane. into the body.

Figure 4(a) shows the film formation on the surface of the material prepared by the preparation method of Comparative Example 1, and (b) shows the film formation on the surface of the material prepared by the preparation method of Comparative Example 2. It can be seen from Figure 4 that when the reinforcement is reinforced The ratio of polysaccharide and hydroformyl polysaccharide is inappropriate - that is, when the ratio of reinforcing polysaccharide and hydroformyl polysaccharide is 1:1 or the ratio of hydroformyl polysaccharide is higher than that of reinforcing polysaccharide, the film formation on the surface of the material is uneven, and the film is broken and lifted Phenomenon, the adhesion effect on the surface is poor and easy to fall off.

Example 6 Antibacterial performance test

Antibacterial performance tests were carried out on the products of Example 2, Example 4 and Example 5, and the measured results are shown in Figure 3 . Antibacterial properties were tested using ASTM E2149 standard. The specific process is to take 4 μL of Escherichia coli into 4 mL of LB medium, incubate it in a shaker at 37°C, incubate Escherichia coli to 1*10 ⁸ CFU/mL, take out and dilute to 1*10 ⁵ CFU/mL, put it in 48 wells The unmodified implants were placed in the plates, the implants modified according to Examples 2, 4, and 5, three parallel samples in each group, and 300 μL of 1*10 ⁵ CFU/mL bacterial solution was added to each well. The orifice plate was placed in a shaker at 37°C and incubated for 12h, then the plate was taken out and 100 μL of bacterial liquid was drawn from each well and placed in a 96-well plate for OD600 determination.

As can be seen from Figure 3, all three materials have good antibacterial properties, and all three groups of materials have achieved 99% sterilization effect, which can achieve fast, efficient and precise sterilization, while the control group (unmodified original implant) has no antibacterial properties. As a result, bacteria multiply on its surface.

Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should Various changes may be made in details without departing from the scope of the invention as defined by the claims.

Claims (10)

1. A preparation method of an intelligent coating on the surface of an orthopedic temporary implant based on natural polysaccharide is characterized by comprising the following steps:

1) reacting the first polysaccharide with sodium periodate to obtain hydroformylation polysaccharide with a hydroformylation degree of 55-65%;

2) dissolving reinforced polysaccharide in hot water to obtain a solution a, dissolving antibiotics in a sodium carbonate solution with the pH value of 9-10 to obtain a solution b, adding the solution b into the solution a, uniformly mixing, and adding the hydroformylation polysaccharide obtained in the step 1) to obtain a film forming solution;

3) placing the orthopedic temporary implant into a reactor, adding the film-forming liquid obtained in the step 2), slowly volatilizing at 30-60 ℃, and uniformly wrapping the surface of the orthopedic temporary implant with a composite film;

4) and (3) soaking the material prepared in the step 3) in a crosslinking agent adding solution for 5-20 min, taking out, washing with water, and drying to obtain the orthopedic temporary implant decorated with the composite membrane.

2. The method for preparing a surface smart coating of an orthopaedic temporary implant based on natural polysaccharides of claim 1, wherein the first polysaccharide of step 1) is cellulose, starch, sodium alginate, sodium hyaluronate, dextran, pullulan, agarose or laminaran, and the reinforcing polysaccharide of step 2) is gelatin, xanthan gum, pectin or acacia.

3. The method for preparing the surface intelligent coating of the orthopaedic temporary implant based on natural polysaccharides as claimed in claim 2, wherein the first polysaccharide in step 1) is sodium alginate and the reinforcing polysaccharide in step 2) is gelatin.

4. The method for preparing the surface intelligent coating of the orthopaedic temporary implant based on the natural polysaccharide is characterized in that the first polysaccharide in the step 1) is 1-15 parts by weight, the sodium periodate is 0.1-5 parts by weight, the hydroformylation polysaccharide in the step 2) is 1-10 parts by weight, the reinforcing polysaccharide is 5-50 parts by weight, the sodium carbonate is 0.5-5 parts by weight, the antibiotic is 0.1-3 parts by weight, and the cross-linking agent in the step 3) is 1-3 parts by weight, wherein the reinforcing polysaccharide is higher than the hydroformylation polysaccharide.

5. The method for preparing the surface intelligent coating of the orthopaedic temporary implant based on the natural polysaccharide according to claim 4, wherein the amount of the hydroformylation polysaccharide in the step 2) is 1-5 parts, and the amount of the reinforcing polysaccharide is 2-10 parts.

6. The method for preparing the surface intelligent coating of the orthopaedic temporary implant based on natural polysaccharides as claimed in claim, wherein the reaction temperature of the reaction of step 1) is 25-37 ℃ and the reaction time is 20-30 h.

7. The method for preparing the smart coating layer on the surface of the orthopaedic temporary implant based on natural polysaccharides as claimed in claim, wherein the temperature of the hot water of step 2) is 60-100 ℃.

8. The method for preparing the surface smart coating of the orthopaedic temporary implant based on natural polysaccharides as claimed in claim 1, wherein the volatilization time of step 3) is 7-10 h.

9. The method for preparing the surface smart coating of the orthopaedic temporary implant based on natural polysaccharides of claim 1, wherein the cross-linking agent of step 4) is genipin, glutaraldehyde or formaldehyde.

10. The material prepared by the method for preparing the surface intelligent coating of the orthopaedic temporary implant based on natural polysaccharides according to any one of claims 1 to 9.

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