CN110764246A - A device for photographing large-area samples on a microscope and a method of using the same - Google Patents

Abstract

The invention discloses a device for photographing large-area samples on a microscope and a method of using the same. Two electric translation stages are arranged on the base of the microscope and are installed orthogonally stacked together, and the slider of the electric translation stage located on the upper part is arranged As a sample placement table; the top of the microscope has an image acquisition sensor, which is used to take pictures of the sample and collect its images; the image acquisition sensor of the microscope is connected to the host computer, and the host computer controls the image acquisition sensor to take pictures, and the image acquisition sensor takes pictures and collects images It is sent back to the upper computer for subsequent image stitching processing; the electric translation stage is connected to the upper computer, and the upper computer controls the movement of the two electric translation stages, thereby controlling the shooting position of the sample. The invention can make full use of the imaging advantages of the optical polarized light microscope, compare the photos of digital cameras, increase the resolution of photos by hundreds of times, and bring breakthroughs to the imaging range of the existing microscopes.

Description

A device for photographing large-area samples on a microscope and a method of using the same

technical field

The invention belongs to the technical field of microscopic image acquisition, and in particular relates to a device for photographing large-area samples on a microscope.

Background technique

At present, the image field of the lowest magnification lens of commercial optical polarizing microscope objective lens is about 1 × 1 cm2, which cannot directly photograph samples larger than centimeter size, which limits the optical characterization of large-sized samples. For large-sized samples, especially crystal samples, although independent polarizers and commercial digital camera lenses can be used to complete the shooting, the contrast and resolution of the photos are difficult to meet the requirements of scientific research, and the microstructure of the samples cannot be captured.

SUMMARY OF THE INVENTION

The purpose of the present invention is to overcome the deficiencies of the prior art and provide a device for photographing large-area samples on a microscope.

The present invention is achieved through the following technical solutions:

A device for shooting large-area samples on a microscope, including a microscope and a host computer. Two motorized translation stages are arranged on the base of the microscope, which are orthogonally stacked and installed together, and the slider of the upper motorized translation stage is used as the sample. placing table;

There is an image acquisition sensor on the top of the microscope, which is used to take pictures of the sample and collect its images; the image acquisition sensor of the microscope is connected to the host computer, and the host computer controls the image acquisition sensor to take pictures, and the image collected by the image acquisition sensor is sent back to the host computer. Perform subsequent image stitching processing;

The electric translation stage is connected with the upper computer, and the upper computer controls the movement of the two electric translation stages, thereby controlling the shooting position of the sample.

In the above technical solution, the microscope is a polarized light microscope.

In the above technical solution, the image acquisition sensor is a CCD image acquisition sensor.

In the above technical solution, the electric translation stage adopts a roller bearing electric translation stage driven by a stepping motor.

In the above technical solution, the host computer uses LabVIEW software to control the moving step length, moving direction and waiting time of the electric translation stage.

The use method of the described apparatus for photographing large-area samples on a microscope is as follows:

Step 1: Place the sample to be photographed on the slider of the upper electric translation stage;

Step 2: Select the objective lens magnification of the microscope, complete the focus, determine a reasonable imaging range, and set the sample moving step, moving direction, waiting time, and the interval time and number of photos on the host computer;

Step 3: According to the control parameters determined in Step 2, the host computer controls the electric translation stage and the CCD image acquisition sensor to complete the image acquisition of the sample;

Step 4: Finally, calibrate the size in the horizontal and vertical directions of the photographed collection, input the calibrated offset into the software, read and locate the photographing coordinates through LabVIEW, and complete the stitching of the photographed images.

The advantages and beneficial effects of the present invention are:

In the invention, a two-axis electronically controlled translation stage is added to the microscope, the translation distance and photographing are controlled by LabVIEW software, and finally a large-area sample microscope image is obtained by piecing together the photographs of each part through the software. This method can make full use of the imaging advantages of optical polarized light microscopy, compare digital camera photos, and increase the resolution of photos by hundreds of times.

Description of drawings

FIG. 1 is a schematic structural diagram of an apparatus for photographing large-area samples on a microscope according to the present invention.

Figure 2 is a schematic diagram of a photo stitching method.

in:

1: The first electric translation stage, 2: The second electric translation stage, 3: The CCD image acquisition sensor.

For those of ordinary skill in the art, other related drawings can be obtained from the above drawings without any creative effort.

Detailed ways

In order to make those skilled in the art better understand the solutions of the present invention, the technical solutions of the present invention are further described below with reference to specific embodiments.

Example 1

Referring to the attached drawings, a device for shooting large-area samples on a microscope, including a polarized light microscope and a host computer, on the base of the polarized light microscope, two motorized translation stages that are vertically stacked and installed together are set, that is, in the polarized light microscope. The base of the light microscope is provided with a first electric translation stage 1 arranged along the Y axis and a second electric translation stage 2 arranged along the X axis, and the second electric translation stage 2 is fixedly installed on the slider of the first electric translation stage 1 , and then the slider of the first electric translation stage 1 can drive the entire second electric translation stage 2 to move along the Y axis, and the slider of the second electric translation stage 2 can move along the X axis, and then the second electric translation stage 2 The slider can adjust the position arbitrarily in the plane.

The slider of the second electric translation stage 2 is used as a sample placement stage. When shooting, the sample is placed on the slider of the second electric translation stage 2, and the slider of the second electric translation stage 2 drives the sample to adjust the shooting position.

The top of the polarized light microscope is provided with a CCD image acquisition sensor 3, which is used for taking pictures of the sample to acquire its image.

The CCD image acquisition sensor of the polarized light microscope is connected to the host computer (PC). The host computer controls the CCD image acquisition sensor to take pictures, and the images captured by the CCD image acquisition sensor are sent back to the host computer for subsequent image stitching processing.

The first electric translation stage 1 and the second electric translation stage 2 are both connected to the upper computer (PC), and the upper computer controls the movement of the first electric translation stage 1 and the second electric translation stage 2, thereby controlling the position of the sample.

Embodiment 2

Further, the first electric translation stage 1 and the second electric translation stage 2 both use roller bearing electric translation stages driven by stepper motors, and the host computer uses LabVIEW software to control the first electric translation stage 1 and the second electric translation stage. The moving step length, moving direction, waiting time of the electric translation stage 2 and the photographing shutter of the CCD image acquisition sensor are triggered.

Embodiment 3

The equipment used to capture large-area samples on the microscope is used as follows:

Step 1: place the sample to be photographed on the slider of the second electric translation stage 2;

Step 2: Select the objective lens magnification of the polarized light microscope, complete the focusing, determine a reasonable imaging range, and set the sample moving step, moving direction, waiting time, and the interval time and number of photos on the host computer;

Step 3: According to the control parameters determined in Step 2, the host computer controls the first electric translation stage 1, the second electric translation stage 2 and the CCD image acquisition sensor to complete the image acquisition of the sample;

Step 4: Finally, calibrate the horizontal and vertical dimensions of the photo collection, input the calibrated offset into the software, read and locate the photo coordinates through LabVIEW, and use the stitching method shown in Figure 2. The selected two-axis electric translation stage is correspondingly designed to complete the automatic stitching of the photos.

For ease of description, spatially relative terms such as "upper", "lower", "left", "right" and the like are used in the embodiments to describe how one element or feature shown in the figures is relative to another element or feature. relation. It should be understood that, in addition to the orientation shown in the figures, spatial terms are intended to encompass different orientations of the device in use or operation. For example, if the device in the figures is turned over, elements described as "below" other elements or features would then be oriented "above" the other elements or features. Thus, the exemplary term "lower" can encompass both upper and lower positions. The device may be otherwise oriented (rotated 90 degrees or at other orientations) and the spatially relative references used herein interpreted accordingly.

Moreover, relational terms such as "first" and "second" etc. are only used to distinguish one element from another having the same name and do not necessarily require or imply any such actual existence between those elements relationship or order.

The present invention has been exemplarily described above. It should be noted that, without departing from the core of the present invention, any simple deformation, modification, or other equivalent replacements that can be performed by those skilled in the art without any creative effort fall into the scope of the present invention. the scope of protection of the invention.

Claims (6)

1. a device for photographing large-area samples on a microscope, is characterized in that: comprise a microscope and a host computer, on the base of the microscope, two orthogonally stacked electric translation stages are arranged to be installed together, and the electric translation stage at the upper part is arranged. The slide of the stage is used as a sample placement stage; There is an image acquisition sensor on the top of the microscope, which is used to take pictures of the sample and collect its images; the image acquisition sensor of the microscope is connected to the host computer, and the host computer controls the image acquisition sensor to take pictures, and the image collected by the image acquisition sensor is sent back to the host computer. Perform subsequent image stitching processing; The electric translation stage is connected with the upper computer, and the upper computer controls the movement of the two electric translation stages, thereby controlling the shooting position of the sample. 2 . The device for photographing large-area samples on a microscope according to claim 1 , wherein the microscope is a polarized light microscope. 3 . 3 . The device for photographing large-area samples on a microscope according to claim 1 , wherein the image acquisition sensor is a CCD image acquisition sensor. 4 . 4 . The device for photographing large-area samples on a microscope according to claim 1 , wherein the electric translation stage adopts a roller bearing electric translation stage driven by a stepping motor. 5 . 5. The device for photographing large-area samples on a microscope according to claim 1, wherein the host computer uses LabVIEW software to control the moving step length, moving direction, and waiting time of the electric translation stage. 6. The using method of the device for photographing large-area samples on a microscope according to claim 1, wherein: Step 1: Place the sample to be photographed on the slider of the upper electric translation stage; Step 2: Select the objective lens magnification of the microscope, complete the focus, determine a reasonable imaging range, and set the sample moving step, moving direction, waiting time, and the interval time and number of photos on the host computer; Step 3: According to the control parameters determined in Step 2, the host computer controls the electric translation stage and the CCD image acquisition sensor to complete the image acquisition of the sample; Step 4: Finally, calibrate the size in the horizontal and vertical directions of the photographed collection, input the calibrated offset into the software, read and locate the photographing coordinates through LabVIEW, and complete the stitching of the photographed images.

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