CN110731757A - A kind of rat penis intracavernous pressure data measurement processing method and measurement device - Google Patents

Abstract

The invention belongs to the technical field of medicine, and discloses a method for measuring and processing data of intracavernous pressure data of a rat penis and a measuring device. The cavernous nerve is electrically stimulated for 1 min and ICP is recorded; the mean arterial pressure is measured, and a PE-50 tube is inserted and connected. Record data to pressure transducer; data processing: collect ICP/MAP and area under the curve AUC. The intracavernous pressure measurement technology of the present invention uses a 10ml syringe needle connected to a PE-50 tube or a 25-G intravenous infusion needle to pierce the sponge strip of the rat penis after anesthesia, isolates the cavernosal nerve located in the dorsal prostate of the rat, and electrically Stimulating the cavernosal nerve to induce the erection of the corpus cavernosum, and processing and recording the ICP value changes through the transducer device, it has the advantages of being intuitive, efficient, and simple in structure, and the pressure measuring device is inexpensive, the experimental results are less subjective, and the experimental results are repeatable, etc. Advantage.

Description

A kind of rat penis intracavernous pressure data measurement processing method and measurement device

technical field

The invention belongs to the technical field of medicine, and in particular relates to a method and a measuring device for measuring and processing data of intracavernous pressure of a rat penis.

Background technique

Currently, the closest existing technology:

Erectile dysfunction (ED) is one of the common male sexual dysfunction diseases, which refers to the difficulty of male patients to achieve or maintain sufficient penile erection to achieve satisfactory sexual performance. Sex life will affect the harmony of the family and cause unavoidable harm to the body and mind.

Penile erection is a neurovascular event that is regulated by a complex neurovascular process. The penis is innervated by both autonomic and somatic nerves. Sympathetic and parasympathetic nerves converge in the pelvis to form cavernosal nerves, enter the corpus cavernosum and glans penis, and regulate the blood flow of the corpus cavernosum. Before erection, neurotransmitters are released into the cavernous vascular tissue and interact with relaxation factors released by vascular endothelial cells. The smooth muscle cells of the cavernous body relax and the arterial vascular resistance decreases, resulting in a marked increase in blood flow into the penis. The relaxation of cavernosal smooth muscle cells increases the compliance of the cavernous sinus and promotes the rapid filling and expansion of the cavernosal body. When highly sexually active, the bulbocavernosus reflex is activated, and the ischiocavernosus muscle contracts strongly to compress the congested cavernous sinus to strengthen the penis, and the inflow and outflow of blood stop. The erection of the penis caused by the stasis of blood in the cavernous body, the pressure in the sinus reaches more than 100mmHg. According to the etiology of various types of ED, domestic and foreign scholars have constructed common related ED animal models (including neurogenic ED, vascular ED, endocrine ED, etc.) for the study of penile erectile function. Rodents are currently the preferred experimental animals in ED animal models.

At present, there are mainly the following methods for the determination of erectile function in experimental animals:

1) Direct observation method of sexual behavior: Apomorphine is a non-selective central dopamine receptor agonist, which can stimulate D ₁ and D ₂ -like receptors and then trigger penile erection. Tan Daqing et al. injected apomorphine into the abdominal cavity of rats and sublingually to simulate clinical ED patients, so as to observe and record the sex hormone levels of experimental male rats, capture female rats per unit time and their ejaculation time, so that apomorphine can help Improve male sexual ability. At the same time, Yang et al. rats were injected with apomorphine (100 μg/kg) subcutaneously in the neck. Penile erection was observed and recorded within 30 minutes after injection. The head of the penis with congestion at the end of the penis was recorded as an erection. The above methods can only indirectly reflect the erection ability of male rats by counting the sexual behavior and penile erection times of male rats after apomorphine intervention, and cannot intuitively compare erection hardness, and are subject to subjective factors of the observer. At the same time, the dose of apomorphine used is uncertain and the multi-target non-selective action of apomorphine may have potentially unknown effects.

2) Physiological telemetry system to monitor intracavernous pressure: At present, many telemetry systems developed by DSI Company in the United States are used (consisting of implants, receivers, and data converters). Power supply, built-in magnetic induction switch, the implant in the rat can be monitored continuously for about 6 months. The disadvantage of this method is that the monitoring of erectile function in rats needs to detect a large number of rats, and the installation of implanted equipment requires high sterility of the operation, requires a lot of manpower and material resources, and the equipment is expensive.

3) Hardness detection by Rigiscan method: This method is more commonly used clinically to detect the penile erection hardness of patients. In the animal erectile function detection test, it is suitable for animals with larger penis size, such as dogs, monkeys, etc. It is not suitable for rat animal models and the detection takes a long time. At the same time, the unit price of the equipment is relatively high, which is not suitable for the simultaneous detection of large quantities of experimental animals.

4) EMG spectrum during measurement of sexual behavior: Miura et al. used electrodes embedded in the ischiocavernosus muscle and bulbocavernosus muscle in advance to record the EMG spectrum of rats during erection to evaluate their penile erection. This method also needs to embed electrodes in advance, which is labor-intensive and time-consuming in large-scale animal experiments.

To sum up, the problems existing in the prior art are:

(1) The data analyzed and detected by the detection method of the prior art are not direct data, but are indirect data and have poor accuracy.

(2) The installation of implanted equipment requires high sterility in surgery, requires a lot of manpower and material resources, and the equipment is expensive.

(3) The detection time of the prior art is relatively long, and the unit price of the detection equipment is relatively high, which is not suitable for the simultaneous detection of a large number of experimental animals.

(4) The prior art detection equipment also needs to embed electrodes in advance, which is labor-intensive and time-consuming in large-scale animal experiments.

(5) The prior art is not applicable to all experimental animals.

(6) Existing apomorphine technology cannot directly compare the degree of erection and the use of drugs to induce erection has other potential unknown effects.

The difficulty of solving the above technical problems:

The research group that measures the erectile function of experimental animals needs to purchase devices for electrophysiological signal detection and bioelectrical signal stimulation, and needs to be proficient in the built-in needles in the corpus cavernosum and the ability to accurately locate and separate the cavernosal nerves.

The significance of solving the above technical problems:

There can be a unified, low-cost, and intuitive method to detect the intracavernosal pressure of experimental animals, and to evaluate the erectile function of experimental animals.

SUMMARY OF THE INVENTION

Aiming at the problems existing in the prior art, the present invention provides a method and a measuring device for measuring and processing data of intracavernous pressure data of a rat penis.

The present invention is realized in this way, a kind of rat penis corpus cavernosum pressure data measurement and processing method, comprising:

Step 1, record ICP for the data of electrical stimulation for 1 min;

Step 2, measuring arterial pressure, inserting PE-50 tube, and connecting to pressure transducer to record data;

Step 3: Collect ICP/MAP and area under the curve AUC and perform data processing.

Further, before step 1 is performed, it is necessary to perform: use 3% sodium pentobarbital to anesthetize the sample.

Further, before step 1 is carried out, it is necessary to carry out:

The cavernous nerves were isolated and marked with PE-10 tubing or sutures.

Further, before step 1 is carried out, it is also necessary to carry out: connecting the built-in tube of the corpus cavernosum to the PE-50 tube or the 25-G infusion tube.

Another object of the present invention is to provide a rat corpus cavernosum pressure data measurement and processing system implementing the rat corpus cavernosum data measurement and processing method, the rat corpus cavernosum data measurement and processing system comprising:

The ICP recording module is used to record the ICP of the data of electrical stimulation for 1 min;

Carotid artery pressure measurement module, used for carotid artery pressure measurement, placed in PE-50 tube, and connected to the pressure transducer to record data;

The data processing module is used to collect ICP/MAP and area under the curve AUC and perform data processing.

Another object of the present invention is to provide a rat corpus cavernosum pressure measuring device, comprising:

Bipolar electrical stimulation hook; the bipolar electrical stimulation hook is connected to the Master-8 programmable stimulator, and the electrical stimulation parameters are 5V, 15Hz, 5ms;

Connect the 10ml syringe needle of PE-50 tube or the tail end of 25-G intravenous infusion needle to the three-way tube and then connect it to the MP160 16-channel physiological recording and analysis system to record ICP;

One end of the three-way tube is connected to a 10ml syringe with 1% heparin saline inside, and the syringe is used to return blood in the PE-50 tube or 25-G intravenous infusion tube connected to the anticoagulation needle.

Another object of the present invention is to provide an information data processing terminal for realizing the method for measuring and processing the data of the intracavernous pressure of the rat.

Another object of the present invention is to provide a computer-readable storage medium, including instructions, which, when run on a computer, cause the computer to execute the method for measuring and processing data of intracavernous pressure of a rat.

To sum up, the advantages and positive effects of the present invention are:

Because rats are cheap and easy to raise, SD rats are generally used as animal models for erectile function analysis. The intracavernous pressure measurement technique uses a 10ml syringe needle connected to a PE-50 tube or a 25-G intravenous infusion needle to pierce the cavernous strip of the rat penis after anesthesia, isolates the cavernosal nerve located in the dorsal prostate of the rat, and conducts electrical stimulation. (parameters are 5V, 15Hz, 5ms) the cavernosal nerve induces corpus cavernosum erection, and the ICP value changes are processed and recorded through the transducer device, which has the advantages of intuition, high efficiency, simple structure, etc., and the pressure measuring device is cheap and the experimental results are less subjective , the experimental results have the advantages of repeatability and so on.

The technical scheme induces congestion and erection of the corpus cavernosum by stimulating the corpus cavernosum with an appropriate electrical signal, detects the pressure signal in the corpus cavernosum through a device inserted into the corpus cavernosum in advance, converts it into an electrical signal through a biological signal transducer, and analyzes it through physiological recording. The ICP curve was obtained after systematic analysis. The required instrument materials include a Master-8 programmable stimulator (AMPI, Israel), an MP160 16-channel physiological recording and analysis system for recording ICP (BIOPAC, USA), a computer, a bipolar electrical stimulation hook, three Cannula, polyethylene PE-50 tube (Smith Medical, UK) + needle or 25-G intravenous infusion needle, 1% heparin saline solution, 3% pentobarbital sodium, animal surgical instruments (scissors, ophthalmic scissors, Microscopic forceps, cotton swabs, arterial clips, rat surgical boards, etc.).

Description of drawings

FIG. 1 is a flow chart of a method for measuring and processing data of intracavernous pressure in a rat penis provided by an embodiment of the present invention.

FIG. 2 is the ICP and MAP measurement results and data statistical histograms of normal rats and type I diabetic rats provided by the embodiment of the present invention, which is intuitive.

Figure 3 is a diagram of a three-way tube provided by an embodiment of the present invention, the top of which is connected to a transducer, the right side is connected to a 5ml syringe filled with anticoagulant heparin, and the left side is connected to an intravenous infusion needle.

In the figure: a, b: localization of the cavernous nerve; c: PE-10 isolation of the cavernous nerve; d, e: needle placement in the corpus cavernosum; f: bipolar electrical stimulation hook and 25-G intravenous infusion needle; g: The actual ICP measurement connection device.

4 is a schematic diagram of a device for measuring intracavernous pressure in a rat penis provided by an embodiment of the present invention.

In the figure: 1. Bipolar electrical stimulation hook; 2. Programmable stimulator; 3. PE-50 tube; 4. Syringe needle; 5. Three-way tube; 6. Physiological recording analysis system; 7. Syringe.

FIG. 5 is a physical diagram of a Master-8 programmable stimulator and a bipolar electrical stimulation hook provided by an embodiment of the present invention.

FIG. 6 is a physical diagram of recording ICP and computer software U disk by the MP160 type 16-channel physiological recording and analysis system provided by the embodiment of the present invention.

Fig. 7 is a graph showing the erection function of experimental rats of different groups in the apomorphine experiment provided by the embodiment of the present invention, which can only be evaluated by counting the number of erections with the naked eye.

FIG. 8 is a visual diagram of adopting the ICP technology provided by an embodiment of the present invention.

FIG. 9 is the MAP data of the normal rat (NC) and the diabetic rat (DM) carotid artery pressure measurement provided in the embodiment of the present invention. Comparison of different material catheters inserted into the carotid artery-PE50 tube and intravenous infusion needle.

FIG. 10 is an ICP experiment diagram of erectile function measurement on a normal group rat (NC) and a bilateral cavernous nerve clip injury erectile dysfunction model group (M) provided by the embodiment of the present invention.

FIG. 11 is a schematic diagram of a system for measuring and processing data of intracavernous pressure in a rat penis provided by an embodiment of the present invention.

In the figure: 8. ICP recording module; 9. Carotid artery pressure measurement module; 10. Data processing module.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

The detection equipment in the prior art has poor data accuracy for analysis and detection. The installation of implanted equipment requires high sterility in surgery, requires a lot of manpower and material resources, and the equipment is expensive. The detection takes a long time, and the unit price of the detection equipment is high, which is not suitable for the simultaneous detection of large quantities of experimental animals. The prior art detection equipment also needs to embed electrodes in advance, which is labor-intensive and time-consuming in large-scale animal experiments.

In view of the problems existing in the prior art, the present invention provides a method and a measuring system for measuring the intracavernous pressure of a rat penis. The present invention will be described in detail below with reference to the accompanying drawings.

As shown in Figure 1, the rat penis intracavernous pressure data measurement and processing method provided in the embodiment of the present invention comprises:

S101, device connection: connect the bipolar electrical stimulation hook to a Master-8 programmable stimulator (AMPI, Israel), and the electrical stimulation parameters are 5V, 15Hz, 5ms. A 10ml syringe needle or 25-G intravenous infusion needle connected to a PE-50 tube, the tail end of which is connected to a tee tube and then connected to the MP160 16-channel physiological recording and analysis system to record ICP. One end of the tee tube is connected to a built-in 1% heparin 10ml syringe of normal saline (for blood return in PE-50 tube or 25-G intravenous infusion tube for anticoagulation needle connection).

S102, rat anesthesia: the rat was anesthetized by intraperitoneal injection of 3% pentobarbital sodium (0.15ml/100g), and the rat was fixed on the operation board in a supine position after anesthesia.

S103, Isolation of the cavernous nerve: After shaving the rat abdomen and sterilizing it with iodophor, make a longitudinal incision of about 3 cm in length near the midline of the rat abdomen, cut the abdominal skin and fascia layer by layer, and bluntly separate the abdominal muscles , After exposure of the abdominal cavity, use a cotton swab to dissect the dorsal prostate tissue and mucosa. The major pelvic ganglia (MPG) is attached to the anterolateral surface of the left and right lobes of the prostate, and the cavernous nerve (CN) vascular bundle from MPG is attached to the prostate and goes down through the urethra to innervate the penis. Use ophthalmic forceps to isolate the cavernous nerve, and use PE-10 tubing or suture to pass under the cavernous nerve as a marker for the next step of electrical stimulation.

S104, Needle inserted into the corpus cavernosum: a longitudinal incision is made in the skin of the penis to expose the head of the penis, the skin and the tunica albuginea are separated, and the corpus cavernosum is exposed on both sides of the penis. Insert the corpus cavernosum parallel to the direction of the corpus cavernosum. When the needle is accurately inserted into the corpus cavernosum, a small amount of blood will return to the head tube connected to the needle when the corpus cavernosum is hyperemia induced by electrical stimulation.

S105, the cavernous nerve is electrically stimulated for 1 min and the ICP is recorded: the operator turns on the switch of the electrical stimulator, holds the bipolar electrical stimulation hook, slightly lifts the fully exposed CN in a suspended state for 1 min, and records the ICP data at the same time. The bipolar electrical stimulation hook should be careful not to touch the pelvic muscles to avoid the influence of muscle contraction on ICP. The main reason is that when the voltage and frequency of electrical stimulation are too high, pelvic floor muscle contraction will be induced, and the contraction of pelvic floor muscle will lead to an abnormal increase in the maximum ICP during erection response. If the bipolar stimulation head is directly placed on the stimulation site for stimulation, it is bound to directly touch and stimulate the pelvic floor muscles and thus affect the measurement results. After the bipolar electrical stimulation hook stimulates the nerve for many times, the top of the electrical stimulation hook will have a blood clot wrapped around the stimulation hook. Therefore, the top of the electrical hook needs to be cleaned in time to ensure that the released electrical stimulation parameters are not disturbed.

S106, mean arterial pressure (MAP) measurement: take a median incision in the neck of the rat, use forceps to bluntly separate the neck muscles layer by layer, expose the left common carotid artery, and then insert PE into the ophthalmic scissors. -50 tube and connect to a pressure transducer to record data.

S107, data processing: ICP/MAP and area under the curve (AUC) are collected.

In the embodiment of the present invention, FIG. 2 is the ICP and MAP measurement results and data statistical histogram of normal rats and type I diabetic rats provided by the embodiment of the present invention, which is intuitive.

In Figure 3: a, b: localization of the cavernous nerve; c: PE-10 isolation of the cavernous nerve; d, e: needle placement in the corpus cavernosum; f: bipolar electrical stimulation hook and 25-G intravenous infusion needle; g : ICP measurement connection device, I is the transducer connected to the MP160 type 16-channel physiological recording and analysis system to record ICP.

As shown in Figure 4, another object of the present invention is to provide a rat corpus cavernosum pressure measuring device including:

Bipolar electrical stimulation hook 1; the bipolar electrical stimulation hook is connected to the Master-8 programmable stimulator 2, and the electrical stimulation parameters are 5V, 15Hz, 5ms.

Connect the 10ml syringe needle 4 of PE-50 tube 3 or the tail end of the 25-G intravenous infusion needle to the tee tube 5 and then connect it to the MP160 type 16-channel physiological recording and analysis system 6 to record ICP;

One end of the three-way tube 5 is connected to a 10ml syringe 7 with 1% heparin saline inside, and the syringe is used to return blood in the PE-50 tube or 25-G intravenous infusion tube connected to the anticoagulation needle.

FIG. 5 is a physical diagram of a Master-8 programmable stimulator and a bipolar electrical stimulation hook provided by an embodiment of the present invention.

FIG. 6 is a physical diagram of recording ICP and computer software U disk by the MP160 type 16-channel physiological recording and analysis system provided by the embodiment of the present invention.

Fig. 7 is a graph showing the erection function of experimental rats of different groups in the apomorphine experiment provided by the embodiment of the present invention, which can only be evaluated by counting the number of erections with the naked eye. ^*** : P<0.001; NC=normal group, DM=diabetic group.

FIG. 8 is a visual diagram of adopting the ICP technology provided by an embodiment of the present invention. ICP data when measuring erectile function in normal rats (NC) and diabetic rats (DM). The penis insertion tube in the ICP measuring device was compared with the PE50 tube of different materials and the intravenous infusion needle.

Fig. 9 is the MAP data of the present invention when the carotid artery internal pressure is measured in normal rats (NC) and diabetic rats (DM). Comparison of different material catheters-PE50 tubes inserted into the carotid artery and intravenous infusion needles.

Figure 10 is a graph of the ICP experiment performed by the author's team on the erectile function measurement of the normal group (NC) and the bilateral cavernosal nerve clip injury erectile dysfunction model group (M).

As shown in Figure 11, the rat corpus cavernosum pressure data measurement and processing system of the present invention includes:

The ICP recording module 8 is used to record the ICP of the data of electrical stimulation for 1 min.

The carotid artery pressure measurement module 9 is used to measure the carotid artery pressure, and is placed in a PE-50 tube and connected to a pressure transducer to record data.

The data processing module 10 is used for collecting ICP/MAP and area under the curve AUC and performing data processing.

In the above-mentioned embodiments, it may be implemented in whole or in part by software, hardware, firmware or any combination thereof. When implemented in whole or in part in the form of a computer program product, the computer program product includes one or more computer instructions. When the computer program instructions are loaded or executed on a computer, all or part of the processes or functions described in the embodiments of the present invention are generated. The computer may be a general purpose computer, special purpose computer, computer network, or other programmable device. The computer instructions may be stored in or transmitted from one computer readable storage medium to another computer readable storage medium, for example, the computer instructions may be downloaded from a website site, computer, server or data center Transmission to another website site, computer, server, or data center by wire (eg, coaxial cable, fiber optic, digital subscriber line (DSL), or wireless (eg, infrared, wireless, microwave, etc.)). The computer-readable storage medium can be any available medium that can be accessed by a computer or a data storage device such as a server, a data center, or the like that includes an integration of one or more available media. The usable media may be magnetic media (eg, floppy disks, hard disks, magnetic tapes), optical media (eg, DVD), or semiconductor media (eg, Solid State Disk (SSD)), among others.

The above descriptions are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the protection of the present invention. within the range.

Claims (8)

1. a rat corpus cavernosum pressure data measurement processing method, is characterized in that, described rat corpus cavernosum pressure data measurement processing method comprises: Step 1, record ICP for the data of electrical stimulation for 1 min; Step 2, measuring arterial pressure, inserting PE-50 tube, and connecting to pressure transducer to record data; Step 3: Collect ICP/MAP and area under the curve AUC and perform data processing. 2. The method for measuring and processing data of intracavernous pressure of rat penis as claimed in claim 1, characterized in that, before step 1 is carried out, it is necessary to carry out: use 3% sodium pentobarbital to carry out anesthesia sample. 3. rat corpus cavernosum pressure data measurement processing method as claimed in claim 1, is characterized in that, before step 1 is carried out, also needs to carry out: The cavernous nerves were isolated and marked with PE-10 tubing or sutures. 4. rat corpus cavernosum pressure data measurement processing method as claimed in claim 1, is characterized in that, before step 1 is carried out, also need to carry out: corpus cavernosum built-in pipe connects PE-50 pipe or 25-G infusion pipe . 5. a rat corpus cavernosum pressure data measuring and processing system implementing the described rat corpus cavernosum data measuring and processing method of claim 1, is characterized in that, described rat corpus cavernosum pressure data measuring and processing system comprises: The ICP recording module is used to record the ICP of the data of electrical stimulation for 1 min; Carotid artery pressure measurement module, used for carotid artery pressure measurement, placed in PE-50 tube, and connected to the pressure transducer to record data; The data processing module is used to collect ICP/MAP and area under the curve AUC and perform data processing. 6. a rat corpus cavernosum pressure measuring device, is characterized in that, described rat corpus cavernosum pressure measuring device comprises: Bipolar electrical stimulation hook; the bipolar electrical stimulation hook is connected with a programmable stimulator, and the electrical stimulation parameters are 5V, 15Hz, 5ms; The 10ml syringe needle connected to the PE tube or the tail end of the intravenous infusion tube is connected to the three-way tube and then connected to the physiological recording and analysis system for recording ICP; One end of the three-way tube is connected to a 10ml syringe with 1% heparin saline inside, and the syringe is used to return blood in the PE tube or 25-G intravenous infusion tube connected to the anticoagulation needle. 7. An information data processing terminal for realizing the method for measuring and processing data of intracavernous pressure data of the rat penis according to claim 1. 8. A computer-readable storage medium, comprising instructions, when executed on a computer, causing the computer to execute the method for measuring and processing data of intracavernosal pressure in a rat as claimed in claim 1.

Images