CN110667908A - A kind of tissue culture seedling packaging method suitable for long-distance transportation - Google Patents

Abstract

The invention discloses a tissue culture seedling packaging method suitable for long-distance transportation, which comprises the following steps: (1) preparation of culture medium; (2) classification and transfer of tissue culture seedlings; (3) packaging: in a foam box Cover the polyethylene or polypropylene film with better light transmittance, place the tissue culture seedlings in the foam box, fold up the film, and tighten it; place an ice pack wrapped with newspaper at the four corners of the foam box, and close the lid of the foam box tightly. Then seal it; then place the foam box in a corrugated box and seal it for packaging; (4) Storage and transportation. The present invention adopts the method of carrying a culture medium for preliminary packaging, groping and screening the components of the culture medium and the containers used, and adopts the methods of wrapping ice packs, multi-layer sealing, etc. to maintain the low temperature environment during transportation, and significantly reduces long-distance transportation. The impact on seedling quality and later survival rate can effectively reduce the pollution rate, facilitate inspection and quarantine, and effectively reduce production costs.

Description

A kind of tissue culture seedling packaging method suitable for long-distance transportation

technical field

The invention relates to the technical field of agricultural planting, in particular to a tissue culture seedling packaging method suitable for long-distance transportation.

Background technique

Tissue culture seedlings are plant seedlings that are highly consistent with the original plant characters obtained under artificial control by using a part of the plant body. They are not affected by natural conditions, have high production efficiency, and have good seedling consistency. It is one of the important seedling types in modern horticulture, protection of endangered plants, development and utilization of new excellent plant resources, etc. However, the tissue culture seedlings, because the plants are young and the vegetative organs such as plant roots, stems and leaves store less nutrients, and the growth environment of the seedlings is changed from a high-humidity and sterile environment to a low-humidity and bacteria-bearing environment after they are out of the bottle. During long-distance transportation It is prone to death, bacterial infection, poor growth and so on. Therefore, how to effectively solve these problems in the long-distance transportation of tissue culture seedlings is a key issue to promote the development of the flower industry including orchids.

With my country's opening to the outside world and the development of international trade in recent years, more and more flowers, seedlings, and germplasm resources are entering and leaving the country, which also increases the risk of the introduction and transmission of harmful organisms. The inspection and quarantine supervision and management of inbound and outbound flowers and seedlings has also become the focus of plant inspection and quarantine work. All countries in the world have strict quarantine on imported plants including tissue culture seedlings, especially the United States, Canada, Australia, South Korea, Japan and other countries. However, due to the uneven production level, management level, and pest control level of flowers and seedlings in my country, as well as the lack of understanding of the rules and methods of phytosanitary in international trade by seedling exporters, export restrictions due to phytosanitary frequently occur. . On the basis of understanding the rules of plant inspection and quarantine, it is of great practical significance to carry out reasonable and scientific packaging for the exported seedlings to achieve smooth customs clearance.

SUMMARY OF THE INVENTION

The present invention overcomes the problems in the prior art that since the tissue culture seedlings are young and the vegetative organs such as roots, stems and leaves of the plant are less nutritious, and the growth environment of the seedlings is changed from a high-humidity and sterile environment to a low-humidity and bacteria-producing environment after they are out of the bottle In the long-distance transportation process, technical problems such as death, bacterial infection, and poor growth are prone to occur, and a packaging method suitable for long-distance transportation of tissue culture seedlings with culture medium is provided.

In order to solve the above problems, the present invention adopts the following technical solutions:

A long-distance transportation and packaging method for tissue culture seedlings with culture medium, comprising the following steps:

(1) Preparation of medium: prepare 1/2 MS medium according to the set formula, put it into a specific container, and then sterilize the container with high pressure steam; after the sterilization is completed, place the container in a clean and cool place In the environment, wait for the medium to solidify for later use; the 1/2 MS medium is prepared by the following method: reducing the amount of a large number of elements in the MS medium to half the original amount, removing the sucrose component, and adding agar 7.0～ 8.0g/L, 0.3～0.5mg/L of Eperon bacteriostatic agent, and the medium can be obtained by adjusting its pH value to 6.0～7.0;

(2) Classification and transfer of tissue culture seedlings: Under the sterile environment of the ultra-clean workbench, transfer the tissue culture seedlings that have completed the rooting stage to the standby medium in step (1), so that the roots of the tissue culture seedlings are inserted into the medium After the transfer is completed, it can be packaged and transported, or the tissue culture seedlings can be temporarily placed in the culture room;

(3) Packing: spread polyethylene or polypropylene film with better light transmittance in a clean foam box, place the tissue culture seedlings transferred in step (2) in the foam box, and fold up the film, Tighten; place ice packs at the four corners of the foam box, then close the lid of the foam box and seal it with transparent tape around the junction of the lid and the box body; then place the foam box in a corrugated box for sealing and packaging;

(4) Storage and transportation: When storing and transporting, place the carton in a shaded and low-temperature environment.

Wherein, the container is a transparent, high temperature and high pressure resistant polypropylene plastic box, the shape is a round bowl, the bottom diameter is 10-14 cm, the top diameter is 15-22 cm, and the height is 5-10 cm. You can choose according to the height of the target seedlings.

Wherein, the ice pack put into the packing box needs to be wrapped in newspaper.

Wherein, the thickness of the plastic film is 5-8 filaments, and the thickness of the culture medium in the container is 1±0.2 cm.

Wherein, in step (4), the shading and low temperature environment is: 4-8°C.

Wherein, in the step (1), the sterilization temperature of the high-pressure steam sterilization is 121-130° C., and the sterilization time is 25-30 minutes.

The present invention is a method for inserting tissue culture seedlings into a medium for packaging and transportation, rather than bare root seedlings for transportation. In the step (1) of the present invention, since the medium used is added with bacteriostatic agents and no sucrose, and technical parameters such as agar dosage and pH are adjusted, the quality of the seedlings to be transported can be guaranteed and the cost can be effectively reduced.

The long-distance transportation and packaging method for tissue culture seedlings with medium of the present invention can be applied to most tissue culture plants, and is especially suitable for orchid plants, such as Paphiopedilum, Dendrobium officinale and the like.

The present invention has the following characteristics:

①The delivery of tissue culture seedlings with culture medium instead of bare root seedlings can effectively reduce the evaporation of water and the significant reduction of relative humidity in the transportation process (from nearly 100% in the culture bottle to the relative humidity of the atmosphere) ), this advantage is more obvious in the process of long-distance transportation; ② the technical invention designs a suitable medium formula, which provides a guarantee for reducing the pollution rate and ensuring the growth of seedlings during transportation; ③ screening specific The container adopts the polypropylene container, which has the characteristics of light transmission, large capacity, light weight and high temperature resistance. The characteristics of light transmission are conducive to the growth of tissue culture seedlings before packaging and after unpacking; large capacity and light weight can reduce the cost of long-distance transportation; high temperature resistance can meet the needs of medium sterilization ( 121°C). ④It has the characteristics of low production cost and convenient operation. The technology of the present invention can effectively reduce the cost of production and transportation by selecting a light-weight large container for loading, selecting 1/2 MS medium and controlling the sub-packing amount (thickness 0.8-1.2 cm), and is easy to operate and easy to popularize and apply. ⑤Designed a scientific and practical packaging method, using plastic film, foam box and carton for three-layer packaging, and using ice packs wrapped with newspapers to reduce the temperature of the environment in the foam box, extending the survival time of the tissue culture seedlings transported. The entire technological process and specific technical measures in the technical invention are suitable for the long-distance transportation of most tissue culture seedlings.

Compared with the prior art, the present invention has the following beneficial effects:

(1) Compared with the transportation of bare root seedlings, the prior art significantly improves the quality of tissue culture seedlings, and is more suitable for long-distance transportation. The so-called bare root seedling transportation, that is, the transportation method without medium, is also the main method of tissue culture seedling transportation at present. The transportation of bare root seedlings has a great impact on the growth potential and survival rate of the seedlings because the tissue culture seedlings are directly transferred from the sterile environment with a humidity of close to 100% to the bacteria-bearing environment with low humidity. The transportation method with culture medium can maintain the original growth state of the seedlings, which can greatly reduce the impact of environmental factors in the transportation process on the tissue culture seedlings and maintain a good growth potential. Especially for long-distance transportation, the advantages of the transportation mode with culture medium are more obvious.

(2) Through the improvement of technical measures, the pollution problem in the transportation process can be effectively reduced, and the good growth potential of the seedlings can be maintained. On the conventional MS basic medium, adding Eperon bacteriostatic agent without sucrose can effectively reduce the growth of bacteria and prevent bacterial infection. Iperon is a long-acting, broad-spectrum, highly active, new type of anti-pollution fungicide for tissue culture. It has strong inhibitory and killing effects on common bacteria, fungi and algae. Adding Iperon can effectively It can inhibit the fungi contained in the original seedlings, and can also prevent the breeding of fungi during transportation. In addition, by adjusting the content of a large amount of elements, the amount of agar and the pH value, on the premise of ensuring the nutrients required for the normal growth of the seedlings, the medium has a certain hardness to prevent the matrix from loosening or flowing during transportation, which affects the state of the seedlings or survive.

(3) Effectively reduce the production cost. The technology of the present invention adopts 1/2 MS basic medium, which reduces the large amount of elements in the MS medium by half, and can reduce the production cost while ensuring the nutritional elements required by the tissue culture seedlings during transportation; at the same time, no sucrose is added to the medium. , and controlling the amount of sub-packaging of the culture medium can directly reduce the production cost; in addition, the use of a large container with a lighter mass to load the container can also effectively reduce the transportation cost.

(4) The technical invention is suitable for long-distance transportation of tissue culture seedlings. The invention designs a scientific and practical packaging method, adopts plastic film, foam box and corrugated carton for three-layer packaging, and uses an ice pack wrapped with newspapers to maintain a low temperature environment in the foam box, which is conducive to maintaining good growth of the seedlings during transportation. It prolongs the survival time of seedlings; at the same time, it is wrapped in newspaper to prevent possible damage to seedlings caused by direct contact with ice packs. The entire technological process and specific technical measures in the technical invention are suitable for long-distance transportation of most tissue culture seedlings, especially for orchids such as Paphiopedilum and Dendrobium officinale.

(5) The seedling packaging method of the present invention is convenient for customs inspection, and is beneficial to the import and export trade of flower seedlings. In the inspection and quarantine of seedlings, the appearance of the material is often judged first, and then the suspected diseased plants are collected and brought to the laboratory for further inspection. In the present invention, by adding bacteriostatic agent ipeiron, not adding sucrose and other measures, the potential fungus pollution is effectively prevented and inhibited; in the packaging process, the three-layer sealed packaging and low temperature environment also help to maintain a clean environment . In addition, the transparent plastic container and culture medium matrix are not only conducive to the contamination inspection by the producer, but also facilitate the inspection by customs personnel during customs clearance.

Detailed ways

The present invention will be further described below in conjunction with examples and tests.

Example 1

A tissue culture seedling packaging method suitable for long-distance transportation, comprising the following steps:

(1) Preparation of medium: prepare 1/2 MS medium according to the set formula, put it into a specific container, and then sterilize the container with high pressure steam; after the sterilization is completed, place the container in a clean and cool place Wait for the medium to solidify in the environment; the 1/2 MS medium is prepared by the following method: reducing the amount of macroelements in the MS medium to half of the original, removing the sucrose component, and adding 8.0 g of agar /L, 0.3mg/L of Eperon bacteriostatic agent, and the medium can be obtained by adjusting its pH value to 7.0; the container is a transparent, high temperature and high pressure polypropylene plastic box, in the shape of a round bowl with a bottom The diameter is 14cm, the top diameter is 15cm, and the height is 10cm; the thickness of the medium in the container is 1.2cm; the sterilization temperature of the high-pressure steam sterilization is 130°C, and the sterilization time is 25min; the 1/2MS medium The formula is as follows: NH ₄ NO ₃ 825mg/L, KNO ₃ 950mg/L, CaCl ₂ 2H ₂ O 220mg/L, MgSO ₄ 7H ₂ O 185mg/L, KH ₂ PO ₄ 85mg/L, KI 0.83mg/ L, H ₃ BO ₃ 6.2 mg/L, MnSO ₄ 4H ₂ O 22.3 mg/L, ZnSO ₄ 7H ₂ O 8.6 mg/L, Na ₂ MnO ₄ 2H ₂ O 0.25 mg/L, CuSO ₄ 5H ₂ O 0.25mg/Lmg/L, CaCl ₂ 6H ₂ O 0.025mg/L, FeSO ₄ 7H ₂ O 27.85mg/L, Na ₂ -EDTA 2H ₂ O 37.3mg/L, Inositol 100mg/L, Niacin 0.5mg/L, Pyridoxine Hydrochloride (Vitamin B6) 0.5mg/L, Thiamine Hydrochloride (Vitamin B1) 0.1mg/L, Glycine 2.0mg/L, Agar 8.0g/L, Eperon Antibacterial Dosage 0.3mg/L, pH 7.0;

(2) Classification and transfer of tissue culture seedlings: Under the sterile environment of the ultra-clean workbench, transfer the tissue culture seedlings that have completed the rooting stage to the standby medium in step (1), so that the roots of the tissue culture seedlings are inserted into the medium After the transfer is completed, it can be packaged and transported, or the tissue culture seedlings can be temporarily placed in the culture room;

(3) Packaging: spread a polypropylene film with better light transmittance in a clean foam box, place the tissue culture seedlings transferred in step (2) in the foam box, fold up the film, and tighten; Place ice packs packed with newspapers on the four corners of the foam box, then close the lid of the foam box and seal the junction of the lid and the box with transparent tape; then place the foam box in a corrugated cardboard box to seal the package; The thickness is 5 wires;

(4) Storage and transportation: When storing and transporting, place the carton in a shaded and low-temperature environment of 8°C.

Example 2

A tissue culture seedling packaging method suitable for long-distance transportation, comprising the following steps:

(1) Preparation of medium: prepare 1/2 MS medium according to the set formula, put it into a specific container, and then sterilize the container with high pressure steam; after the sterilization is completed, place the container in a clean and cool place Wait for the medium to solidify in the environment; the 1/2 MS medium is prepared by the following method: reducing the amount of a large amount of elements in the MS medium to half of the original, removing the sucrose component, and adding 7.0 g of agar /L, 0.5mg/L of Eperon bacteriostatic agent, and the medium can be obtained by adjusting its pH value to 6.0; the container is a transparent, high-temperature and high-pressure-resistant polypropylene plastic box, in the shape of a round bowl with a bottom The diameter is 14cm, the top diameter is 15cm, and the height is 10cm; the thickness of the medium in the container is 0.8cm; the sterilization temperature of the high-pressure steam sterilization is 121°C, and the sterilization time is 30min; the 1/2MS medium The formula is as follows: NH ₄ NO ₃ 825mg/L, KNO ₃ 950mg/L, CaCl ₂ 2H ₂ O 220mg/L, MgSO ₄ 7H ₂ O 185mg/L, KH ₂ PO ₄ 85mg/L, KI 0.83mg/ L, H ₃ BO ₃ 6.2 mg/L, MnSO ₄ 4H ₂ O 22.3 mg/L, ZnSO ₄ 7H ₂ O 8.6 mg/L, Na ₂ MnO ₄ 2H ₂ O 0.25 mg/L, CuSO ₄ 5H ₂ O 0.25mg/Lmg/L, CaCl ₂ 6H ₂ O 0.025mg/L, FeSO ₄ 7H ₂ O 27.85mg/L, Na ₂ -EDTA 2H ₂ O 37.3mg/L, Inositol 100mg/L, Niacin 0.5mg/L, Pyridoxine Hydrochloride (Vitamin B6) 0.5mg/L, Thiamine Hydrochloride (Vitamin B1) 0.1mg/L, Glycine 2.0mg/L, Agar 7.0g/L, Eperon Antibacterial Dosage 0.5mg/L, pH 6.0;

(2) Classification and transfer of tissue culture seedlings: Under the sterile environment of the ultra-clean workbench, transfer the tissue culture seedlings that have completed the rooting stage to the standby medium in step (1), so that the roots of the tissue culture seedlings are inserted into the medium After the transfer is completed, it can be packaged and transported, or the tissue culture seedlings can be temporarily placed in the culture room;

(3) Packing: lay a polyethylene film with better light transmittance in a clean foam box, place the tissue culture seedlings transferred in step (2) in the foam box, gather up the film, and tighten; Place ice packs packed with newspapers on the four corners of the foam box, then close the lid of the foam box and seal the junction of the lid and the box with transparent tape; then place the foam box in a corrugated cardboard box to seal the package; The thickness is 8 wires;

(4) Storage and transportation: During storage and transportation, place the carton in a shaded and low-temperature environment at 4°C.

Example 3

A tissue culture seedling packaging method suitable for long-distance transportation, comprising the following steps:

(1) Preparation of medium: prepare 1/2 MS medium according to the set formula, put it into a specific container, and then sterilize the container with high pressure steam; after the sterilization is completed, place the container in a clean and cool place Wait for the medium to solidify in the environment; the 1/2 MS medium is prepared by the following method: reducing the amount of a large amount of elements in the MS medium to half of the original, removing the sucrose component, and adding 7.5 g of agar /L, 0.4mg/L of Eperon bacteriostatic agent, the medium can be obtained by adjusting its pH value to 6.5; the container is a transparent, high temperature and high pressure polypropylene plastic box, the shape is a round bowl, the bottom The diameter is 12 cm, the top diameter is 20 cm, and the height is 8 cm; the thickness of the medium in the container is 1 cm; the sterilization temperature of the high-pressure steam sterilization is 125 ° C, and the sterilization time is 28 min; The formula is as follows: NH ₄ NO ₃ 825mg/L, KNO ₃ 950mg/L, CaCl ₂ 2H ₂ O 220mg/L, MgSO ₄ 7H ₂ O 185mg/L, KH ₂ PO ₄ 85mg/L, KI 0.83mg/L , H ₃ BO ₃ 6.2mg/L, MnSO ₄ 4H ₂ O 22.3mg/L, ZnSO ₄ 7H ₂ O 8.6mg/L, Na ₂ MnO ₄ 2H ₂ O 0.25mg/L, CuSO ₄ 5H ₂ O 0.25mg/Lmg/L, CaCl ₂ 6H ₂ O 0.025mg/L, FeSO ₄ 7H ₂ O 27.85mg/L, Na ₂ -EDTA 2H ₂ O 37.3mg/L, Inositol 100mg/L, Tobacco Acid 0.5mg/L, pyridoxine hydrochloride (vitamin B6) 0.5mg/L, thiamine hydrochloride (vitamin B1) 0.1mg/L, glycine 2.0mg/L, agar 7.5g/L, eperon bacteriostatic agent 0.4mg/L, pH 6.5;

(2) Classification and transfer of tissue culture seedlings: Under the sterile environment of the ultra-clean workbench, transfer the tissue culture seedlings that have completed the rooting stage to the standby medium in step (1), so that the roots of the tissue culture seedlings are inserted into the medium After the transfer is completed, it can be packaged and transported, or the tissue culture seedlings can be temporarily placed in the culture room;

(3) Packing: lay a polyethylene film with better light transmittance in a clean foam box, place the tissue culture seedlings transferred in step (2) in the foam box, gather up the film, and tighten; Place ice packs packed with newspapers on the four corners of the foam box, then close the lid of the foam box and seal the junction of the lid and the box with transparent tape; then place the foam box in a corrugated cardboard box to seal the package; The thickness is 7 wires;

(4) Storage and transportation: During storage and transportation, place the carton in a shaded and low-temperature environment at 6°C.

control group 1

The difference between the method of transporting tissue culture seedlings in control group 1 and Example 1 is that in control group 1, the tissue culture seedlings are placed in a plastic container without culture medium, and a layer of moist paper towels is placed underneath. That is: use the improved bare root seedlings for transportation.

The transportation mode of the tissue culture seedlings of the control group 2 is basically the same as that of the embodiment 1, the difference is that the culture medium adopted by the control group 2 does not add the Eperon bacteriostatic agent, and contains sucrose, and the amount of sucrose is 30g/L, and the remaining components and The content is the same as that of the 1/2 MS medium of Example 1.

control group 3

The transportation method of the tissue culture seedlings of the control group 3 is basically the same as that of the embodiment 1, the difference is that, in the packaging of the step (3) of the control group 3, four wrapped ice packs are not placed at the four corners of the foam box, and only the two opposite ones are placed. Place two wrapped ice packs in the corners.

control group 4

The transportation mode of the tissue culture seedlings of the control group 4 is basically the same as that of the embodiment 1, the difference is that the content of the agar contained in the medium formula adopted by the control group 4 is 5.5g/L, and the remaining components and their contents are the same as those of the embodiment 1. /2MS medium is the same.

control group 5

The transportation method of tissue culture seedlings in control group 5 is basically the same as that in Example 1, except that the medium formula used in control group 5 is MS medium, and the thickness of the medium is 1.5 cm.

1. Transport tissue culture seedling test

According to the transportation methods of tissue culture seedlings provided in Examples 1 to 3 and Control Group 1 to Control Group 5, P. The growth status of tissue culture seedlings after unboxing and the survival rate after transplanting are shown in Table 1 below.

Table 1 Growth vigor and transplanting survival rate of the packaged seedlings stored at room temperature for 10 days

It can be seen from Table 1 that the cultured seedlings in control group 1 were severely wilted, the growth potential was poor, and the survival rate after transplanting was low, which was related to the loss of water during storage; the bacterial and fungal contamination of the cultured seedlings in control group 2 was more obvious and seriously affected. The quality of the seedlings and the survival rate in the later period were measured; the tissue culture seedlings in control group 3 had yellow leaves and leggy growth, indicating that two ice packs were not enough to maintain the low temperature environment within 10 days, which would affect the quality and survival rate of seedlings. The medium of control group 4 is easy to loose and overturn, which is not conducive to transportation, thus affecting the consistency and survival rate of tissue culture seedlings. The tissue culture seedlings in control group 5 grew well, but the production cost and transportation cost increased significantly. On the other hand, the tissue culture seedlings of Examples 1 to 3 have bright green leaves, no wilting, good growth, and high survival rate (above 98%), and the production costs of Examples 1 to 3 are relatively low. It shows that the tissue culture seedling packaging method of the present invention can keep the tissue culture seedlings in good growth potential during long-distance transportation (more than 10 days), and has outstanding technical effect.

The above description is a detailed description of the preferred feasible embodiments of the present invention, but the embodiments are not intended to limit the scope of the patent application of the present invention. All equivalent changes or modifications completed under the technical spirit suggested by the present invention shall belong to This invention covers the scope of the patent.

Claims (6)

1. A tissue culture seedling packing method suitable for long-distance transportation is characterized by comprising the following steps:

(1) preparing a culture medium: preparing 1/2MS culture medium according to a set formula, subpackaging into specific containers, and sterilizing the containers with high pressure steam; after the sterilization is finished, placing the container in a clean and cool environment to solidify the culture medium for later use; the 1/2MS culture medium is prepared by the following method: reducing the dosage of macroelements in the MS culture medium to one half of the original dosage, removing sucrose components, adding 7.0-8.0 g/L of agar and 0.3-0.5 mg/L of Yipeilong bacteriostat, and adjusting the pH value to 6.0-7.0 to obtain the culture medium;

(2) grading and transferring the tissue culture seedlings: transferring the tissue culture seedlings after the rooting stage to the standby culture medium in the step (1) in an aseptic environment of a superclean workbench, inserting the roots of the tissue culture seedlings into the culture medium, and grading according to the sizes of plants; after the transfer is finished, the tissue culture seedlings can be packaged and transported, and can also be temporarily placed in a culture room;

(3) packaging: laying a polyethylene or polypropylene film with good light transmittance in a clean foam box, placing the tissue culture seedling subjected to the transfer in the step (2) in the foam box, and gathering and tightening the film; placing ice bags at four corners of the foam box, tightly covering the cover of the foam box, and sealing the joint of the cover and the box body by using transparent adhesive tapes; then the foam box is placed in a corrugated paper box for sealed packaging;

(4) storage and transportation; during storage and transportation, the carton is placed in a shady and low-temperature environment.

2. The method for packaging tissue culture seedlings suitable for long-distance transportation according to claim 1, wherein the container is a transparent, high-temperature and high-pressure resistant polypropylene plastic box shaped as a round bowl; the diameter of the bottom of the container is 10-14 cm, the diameter of the top of the container is 15-22 cm, and the height of the container is 5-10 cm.

3. The method for packaging tissue culture plantlets suitable for long-distance transportation according to claim 1, wherein the ice bag put into the packaging box needs to be wrapped by newspaper.

4. The method for packaging tissue culture plantlets suitable for long-distance transportation according to claim 1, wherein the thickness of the plastic film is 5-8 filaments, and the thickness of the culture medium in the container is 1 ± 0.2 cm.

5. The method for packaging tissue culture seedlings suitable for long-distance transportation according to claim 1, wherein in the step (4), the shaded low-temperature environment is: 4-8 ℃.

6. The method for packaging tissue culture seedlings suitable for long-distance transportation according to claim 1, wherein in the step (1), the sterilization temperature of the high-pressure steam sterilization is 121-130 ℃, and the sterilization time is 25-30 min.