CN110652533A - Antibacterial composition and application thereof - Google Patents

Abstract

The invention provides a bacteriostatic composition and application thereof, wherein the composition comprises the following components in parts by weight: 2-6 parts of trehalose, 1-5 parts of tartaric acid, 0.3-0.7 part of dandelion extract and 0.1-0.5 part of paeonol; according to the invention, active components are selected, the formula is optimized, the components are matched with each other to promote, the synergy is realized, the antibacterial effect is widely and durably realized, the moisture retention and the moistening are realized, the preparation method is used for preparing the disinfection gel, the convenience and the rapidness are realized, the disinfection effect is good, the process is simple, the use is convenient, good economic benefits can be brought to enterprises, and the prospect is wide.

Description

Antibacterial composition and application thereof

Technical Field

The invention belongs to the technical field of disinfection, and relates to a bacteriostatic composition and application thereof.

Background

The transmission pathway of bacterial and viral infections is common as contact transmission, with hand being the most important transmission medium, and hand disinfection is therefore the most important measure for effective prevention and control of nosocomial infections. In the numerous medical and nursing work of medical staff, many work is completed by hands, the number of various bacteria on the hands of the medical staff is often more than that of other people, and the microbial contamination of the hands is serious. Cross-infection caused by hand contamination of medical personnel is one of the major risk factors for nosocomial infections. Daily adherence of medical personnel to high quality hand washing and disinfection can reduce the incidence of nosocomial infections. The problem of nosocomial infection is increasingly prominent, and the nosocomial infection becomes a public health problem which is urgently needed to be solved by hospitals.

At present, the hand disinfectants in the market are various in types, and the disinfection effect and the practicability are good and uneven; conventional hand washing methods use soap and water to remove dirt and harmful microorganisms from the skin surface, however if the hand water is not completely dried through, the residual water can become a harborage of bacteria. This situation poses no serious threat to the general population, but the possibility of infection to the population in the hospital is high, which makes it highly desirable to minimize the risk of contamination.

The quick-drying hand disinfectant is recommended to be a preferred hand disinfection method because of the advantages of quick disinfection, no residue, no irritation, convenient use, working time saving, work efficiency improvement and the like, and the gel type hand quick disinfectant is a hand disinfection product sold in the market in recent years, has the characteristics of good sterilization effect, quick action speed, good skin adaptability, no leakage and no splashing in use, is well received by medical staff, and is considered as an important measure for ensuring that the medical staff keeps hand hygiene constantly. However, with the development of medical technology, higher requirements are put forward on skin disinfection gel used in cooperation, and virus propagation and infection caused by pathogenic bacteria residue are reduced to the maximum extent, so that the traditional skin disinfection gel cannot meet the development of medical requirements, and the improvement of the sterilization effect and the disinfection effect becomes a problem to be solved urgently in development and application.

CN105362212A relates to a hand-washing-free disinfection gel and a preparation method thereof, which comprises 0.05-2% of cationic bactericide, 0.0005-0.02% of anionic surfactant or zwitterionic surfactant, 50-80% of ethanol, 0.05-1.5% of thickening agent, 0.1-1% of skin-care humectant, 0.2-1% of neutralizer and the balance of water, but the invention has no lasting antibacterial effect and needs to be further improved. CN106215142A provides a traditional Chinese medicine medical disinfection gel, which is prepared from the following main raw materials in parts by weight: 5-10 parts of mint, 5-10 parts of cucurbita pepo, 10-20 parts of fructus cnidii, 10-15 parts of coptis chinensis, 5-12 parts of dried ginger, 13-16 parts of golden cypress, 5-9 parts of rhizoma cimicifugae, 4-8 parts of gypsum, 5-8 parts of mung bean, 8-15 parts of exocarpium benincasae, 30-45 parts of hydroxypropyl methyl cellulose, 38-43 parts of polyvinyl alcohol, 10-20 parts of abelmoschus manihot gum and 3-5 parts of glycerol. The invention adds too many traditional Chinese medicine components, has bad smell and bad use experience. CN104547275A discloses a hand essential oil skin-care disinfecting gel, which is prepared from liquid raw materials and solid raw materials, wherein the liquid raw materials comprise the following raw materials in percentage by volume: 55-70% of ethanol, 0.5-1% of an essential oil mixture, 1-5% of glycerol, 0.05-0.1% of triethanolamine and 29-40% of purified water; the solid material comprises triclosan and carbomer, wherein 0.3-0.6g triclosan and 0.15-0.4g carbomer are added into 100ml liquid material. But the invention takes chemical components as main sterilization factors, has single effect and has a non-wide bacteriostatic range.

Therefore, the long-acting antibacterial skin-moistening hand-washing-free disinfection gel is developed, is applied to the fields of daily life and medical care, and has wide application prospect and great market value.

Disclosure of Invention

The invention provides an antibacterial composition and application thereof, wherein active ingredients are selected, a formula is optimized, the components are matched with each other to promote, synergize, and carry out wide and lasting antibacterial action.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the invention provides a bacteriostatic composition, which comprises the following components in parts by weight: 2-6 parts of trehalose, 1-5 parts of tartaric acid, 0.3-0.7 part of dandelion extract and 0.1-0.5 part of paeonol.

In the invention, in the long-term scientific research and practice process, the inventor deeply studies the advantages and the disadvantages of the no-hand sanitizer in the market, develops a long-lasting antibacterial and skin-moistening disinfectant gel, selects active components, optimizes the composition formula, mutually promotes the matching of trehalose and tartaric acid, inhibits bacteria in a broad spectrum, moisturizes and moistens skin, removes heat and toxic materials from dandelion extract glycoside, improves the activity of resisting germs of the skin, relieves pain, resists inflammation, lightens and whitens the skin, promotes the blood circulation of the skin, is combined and matched with the trehalose and the dandelion extract, obviously moisturizes and tenders the skin, mutually cooperates and promotes the components, synergizes, widely and durably inhibits bacteria, moisturizes, lightens and moistens the skin, is used for preparing the disinfectant gel, is convenient and fast, has a good disinfection effect, and has a wide application prospect and a huge market.

Preferably, the composition comprises the following components in parts by weight: 2-4 parts of trehalose, 1-3 parts of tartaric acid, 0.4-0.6 part of dandelion extract and 0.1-0.3 part of paeonol.

The trehalose may be, for example, 2 parts, 3 parts, 4 parts, 5 parts or 6 parts by weight.

Trehalose is also called rhaponticum uniflorum sugar, mycose and the like, is a safe and reliable natural saccharide, and has nonspecific protection effect on various bioactive substances. The trehalose can form a unique protective film on the cell surface under severe environmental conditions such as high temperature, high cold, high osmotic pressure, drying and dehydration, and the like, so that the protein molecules are effectively protected from invariance and inactivation, and the life process and biological characteristics of a living body are maintained. The unique functional characteristic makes trehalose as an excellent activity protective agent for protein drugs, enzymes, vaccines and other biological products, and also an important component for maintaining cell activity and moisturizing cosmetics. In the composition provided by the invention, besides the skin moisturizing effect of the trehalose, the trehalose is matched with the paeonol and the dandelion extract to mutually promote, synergistically enhance the cell activity of the skin, resist melanin deposition, whiten and moisten the skin.

The weight portion of the tartaric acid may be, for example, 1 part, 2 parts, 3 parts, 4 parts, or 5 parts.

Tartaric acid is a carboxylic acid, is present in a variety of plants, such as grapes and tamarind, can be used in food, and has a bactericidal effect on gram-positive bacteria and some gram-negative bacteria.

The dandelion extract can be, for example, 0.3 part, 0.4 part, 0.5 part, 0.6 part or 0.7 part by weight.

Taraxacum genus Compositae family perennial herb. The head-shaped inflorescence, the seeds are provided with pompon formed by white crown hairs, and the pompon floats to a new place along with wind after the flowers bloom to inoculate new life. The dandelion extract contains a plurality of healthy nutritional ingredients such as flavonoid, dandelion alcohol, taraxacin, choline, organic acid, inulin and the like, has the functions of clearing away heat and toxic materials, resisting bacteria and diminishing inflammation, has stronger bactericidal action on staphylococcus aureus and hemolytic streptococcus, also has inhibiting effect on most dermatophytes, can resist oxygen free radicals and prevent melanin deposition; the dandelion extract with a specific part is added into the composition, so that the dandelion composition has the antibacterial and anti-inflammatory effects, and is matched with trehalose and paeonol to jointly promote the whitening, moistening and melanin deposition resisting effects on skin.

The paeonol can be 0.1 part, 0.2 part, 0.3 part, 0.4 part or 0.5 part by weight.

Paeonol is an effective component extracted from dried root bark of Paeonia suffruticosa Andr of Ranunculaceae, and has effects of relieving pain, resisting bacteria, resisting oxidation, resisting inflammation and relieving fever, inhibiting generation of intracellular oxygen free radical, whitening skin, reducing and discoloring pigment deposited in skin, removing blood stasis and resolving macula; simultaneously, the face cream has the functions of diminishing inflammation, relieving swelling and pain, resisting allergy, resisting virus and the like, can promote the blood circulation of facial skin, activate aged epidermal cells, eliminate wrinkles, remove spots, moisten skin, beautify the face, promote the metabolism of the skin, improve the skin color and remove acnes; the paeonol with a specific part is added into the composition and is matched with other components, so that on one hand, the antibacterial and anti-inflammatory effects of tartaric acid and dandelion extract are improved, on the other hand, the trehalose is matched, the whitening and moisturizing effects are realized, the skin is tender, and the effects of the composition are improved in multiple directions.

Preferably, the composition further comprises 3-5 parts of ginger extract.

The weight portion of the ginger extract can be 3 parts, 4 parts or 5 parts, for example.

The rhizoma Zingiberis recens extract contains gingerol, shogaol, bisabolene, linalool, and eucalyptol; in addition, the spicy gingerol, the decomposition product of gingerone and a plurality of amino acids have the functions of antibiosis, disinsection and antiphlogosis, and also have strong oxidation resistance.

According to the invention, the ginger extract is matched with the dandelion extract, so that the effects of clearing heat, removing toxicity, promoting each other, maximally playing the antibacterial and anti-inflammatory effects, enhancing the self resistance of skin together and comprehensively improving the antibacterial effect of the composition are achieved.

Preferably, the composition comprises 2-4 parts of trehalose, 1-3 parts of tartaric acid, 0.1-0.3 part of paeonol, 0.4-0.6 part of dandelion extract and 3-5 parts of ginger extract.

In a second aspect, the present invention provides the use of a composition according to the first aspect for the preparation of a disinfecting gel.

In a third aspect, the present invention provides a no-wash disinfecting gel comprising the composition of any one of claims 1-4;

preferably, the composition is present in the antiseptic gel in an amount of 3% to 5% by weight, for example 3%, 3.5%, 4%, 4.5% or 5%.

Preferably, the disinfecting gel further comprises 0.5% -1.5% of chlorhexidine digluconate, 0.03% -0.06% of triethanolamine, 0.2% -0.25% of carbomer and 1.9% -1.95% of propylene glycol in percentage by mass.

Preferably, the disinfection gel further comprises 50% -66% of ethanol, 0.9% -1.0% of glycerol and 0.9% -1.0% of solubilizer by mass percentage.

Preferably, the disinfection gel comprises the following components in percentage by mass: 3 to 5 percent of bacteriostatic composition, 0.5 to 1.5 percent of chlorhexidine digluconate, 0.03 to 0.06 percent of triethanolamine, 0.2 to 0.25 percent of carbomer, 1.9 to 1.95 percent of propylene glycol, 50 to 66 percent of ethanol, 0.9 to 1.0 percent of glycerol, 0.9 to 1.0 percent of solubilizer and the balance of water;

the composition comprises the following components in parts by weight: 2-4 parts of trehalose, 1-3 parts of tartaric acid, 0.1-0.3 part of paeonol, 0.4-0.6 part of dandelion extract and 3-5 parts of ginger extract.

In the invention, in order to develop the convenient-to-use and durable antibacterial hand-washing-free disinfection gel, the formula is optimized on the basis of the prior art, the antibacterial composition is matched with ethanol and chlorhexidine digluconate as main components for disinfection and sterilization, the main components are added into carbomer and triethanolamine to form a gel shape, and the components are mutually matched to promote the antibacterial composition to exert the effect and maintain the stability of active factors.

In a fourth aspect, the present invention provides a method of preparing a sterilising gel as defined in the third aspect, comprising the steps of:

(1) weighing carbomer and ethanol in proportion, and fully swelling;

(2) adding triethanolamine into the product swelled in the step (1) in proportion, and stirring into gel;

(3) mixing and dissolving chlorhexidine digluconate, antibacterial composition, solubilizer, glycerol and propylene glycol according to a certain proportion;

(4) and (3) adding the product obtained in the step (3) into the product obtained in the step (2) for dissolving to obtain the disinfection gel.

Compared with the prior art, the invention has the following advantages:

the antibacterial composition provided by the invention has the advantages of lasting broad spectrum antibacterial effect, whitening and tendering skin, moistening and moisturizing, combination of multiple active components, mutual promotion and synergistic sterilization, capability of enhancing skin activity, broad-spectrum inhibition of bacterial virus breeding, convenience in use, quick drying and no-washing, capability of avoiding the defect that an ethanol disinfectant brings loss to skin, promotion of the antibacterial effect of the disinfection gel, wide application prospect and great market value.

Detailed Description

To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

Example 1

Weighing the antibacterial composition: 3 parts of trehalose, 2 parts of tartaric acid, 0.5 part of dandelion extract, 0.3 part of paeonol and 4 parts of ginger extract;

(1) weighing 0.21% carbomer and 60% ethanol, and swelling completely;

(2) adding 0.05% triethanolamine into the product swelled in the step (1), and stirring to form gel;

(3) mixing and dissolving 1.0% of chlorhexidine digluconate, 4% of bacteriostatic composition, 0.96% of solubilizer, 0.96% of glycerol and 1.92% of propylene glycol;

(4) and (3) adding the product obtained in the step (3) into the product obtained in the step (2) for dissolving to obtain the disinfection gel.

Example 2

Weighing the antibacterial composition: 2 parts of trehalose, 5 parts of tartaric acid, 0.3 part of dandelion extract, 0.5 part of paeonol and 3 parts of ginger extract;

(1) weighing 0.2% carbomer and 50% ethanol, and swelling completely;

(2) adding 0.03% triethanolamine into the product swelled in the step (1), and stirring to form gel;

(3) mixing and dissolving 1.5% of chlorhexidine digluconate, 3% of bacteriostatic composition, 1.0% of solubilizer, 0.9% of glycerol and 1.9% of propylene glycol;

(4) and (3) adding the product obtained in the step (3) into the product obtained in the step (2) for dissolving to obtain the disinfection gel.

Example 3

Weighing the antibacterial composition: 6 parts of trehalose, 1 part of tartaric acid, 0.7 part of dandelion extract, 0.1 part of paeonol and 5 parts of ginger extract;

(1) weighing 0.25% carbomer and 66% ethanol, and swelling completely;

(2) adding 0.06% triethanolamine into the product swelled in the step (1), and stirring to form gel;

(3) mixing and dissolving 0.5% of chlorhexidine digluconate, 5% of bacteriostatic composition, 1.0% of solubilizer, 1.0% of glycerol and 1.95% of propylene glycol;

(4) and (3) adding the product obtained in the step (3) into the product obtained in the step (2) for dissolving to obtain the disinfection gel.

Comparative example 1

The conditions were the same as in example 1 except that ginger extract was not added, compared to example 1.

Comparative example 2

The same conditions as in example 1 were used except that no paeonol was added, compared with example 1.

Comparative example 3

The conditions were the same as in example 1 except that no dandelion extract was added as compared with example 1.

Comparative example 4

The conditions were the same as in example 1 except that no tartaric acid was added as compared with example 1.

Comparative example 5

The conditions were the same as in example 1 except that trehalose was not added, compared with example 1.

Comparative example 6

The conditions were the same as in example 1 except that the amount of tartaric acid added was changed to 7 parts as compared with example 1.

Comparative example 7

The conditions were the same as in example 1 except that the addition amount of trehalose was changed to 8 parts as compared with example 1.

Comparative example 8

The same conditions as in example 1 were used except that the amount of paeonol added was changed to 1 part as compared with example 1.

Comparative example 9

The conditions were the same as in example 1 except that the amount of the dandelion extract was changed to 1 part as compared with example 1.

Experimental detection

Skin irritation test

Selecting 9 adult healthy SD rats, and shearing the back hairs on two sides of the spine of the experimental animal, wherein the hair removing range is 1.5cm multiplied by 1.5 cm; 0.3mL of the gel prepared in examples 1-3 was applied directly to the skin, covered and fixed with two layers of gauze (2 cm. times.2 cm) and one layer of non-irritating oilpaper, the other side serving as a control. After 2h of stimulation, removing residual test substances by using deionized water; the application is carried out for 7 days 1 time per day, and the result is observed 12h after each application.

The result shows that after the gel is continuously applied for 7 days, no erythema or red swelling is seen on the skin on the administration side, and no abnormality is seen compared with the skin on the control side, which indicates that the gel is mild and non-irritant to the skin.

Disinfection effect monitoring

The disinfection effect detection is carried out on the examples and the comparative examples according to the carrier soaking quantitative sterilization test in the technical specification 2002 edition of the disinfectant, the average value of the killing time and the killing rate of three bacteria including staphylococcus aureus, escherichia coli and pseudomonas aeruginosa is calculated, meanwhile, the bacteriostasis time with the bacteria proliferation rate lower than 10% is detected, and the result is shown in the table 1;

TABLE 1

As can be seen from Table 1, the gels prepared in examples 1-3 have short killing time and high killing rate, wherein the gel prepared in example 1 has the best effect, the killing rate is as high as 97.25%, the bacteriostasis time is as long as 23h, while the gel prepared in comparative example 1 lacks ginger extract, and the killing rate is still higher than 88.43%; the gel of comparative examples 2 to 5 lacks any one of the four components, the bacteriostasis time is obviously shortened, and the killing rate is obviously reduced, while the addition amount of the components in the gel of comparative examples 6 to 9 exceeds the range, the bacteriostasis time and the sterilization rate of the gel are also influenced, which shows that in the technical scheme of the application, the four components of the composition lack one and can not cooperate with each other in a specific range, the synergy is realized, and the bacteriostasis time and the sterilization rate are improved.

Sensory evaluation test

Selecting 120 men and women with skin problems at 20-50 years old, randomly dividing into 12 groups, continuously using the products of examples 1-3 and comparative examples 1-9 for 1 month, and evaluating the using effect, wherein the results are shown in Table 2;

TABLE 2

As can be seen from table 2, the total sensory evaluation scores of the gels prepared according to the technical scheme provided by the invention in examples 1-3 are excellent, and the skin repairing capability is excellent, while the different indexes of the sensory evaluation are reduced in different degrees due to the lack of any component or the exceeding of the addition of the component in comparative examples 1-9, and the core formula component is adopted in comparative example 7, only the amount of trehalose is increased, and the low irritation and good moistening and moisturizing effects can be still realized; the comparative examples 1 to 5 lack any component, so that the active effects of the raw material components cannot be exerted to the maximum extent, and the repair performance of the product is obviously reduced, which shows that the composition provided by the invention is lack of any component and has synergistic effect.

In conclusion, the invention provides the antibacterial composition and the application thereof, the active ingredients are selected, the formula is optimized, the components are matched with each other to promote, synergize and inhibit bacteria widely and durably, and the antibacterial composition is used for preparing the disinfection gel, is convenient and quick, has good disinfection effect, wide application prospect and great market value.

The applicant states that the present invention is illustrated in detail by the above examples, but the present invention is not limited to the above detailed methods, i.e. it is not meant that the present invention must rely on the above detailed methods for its implementation. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims (10)

1. The bacteriostatic composition is characterized by comprising the following components in parts by weight: 2-6 parts of trehalose, 1-5 parts of tartaric acid, 0.3-0.7 part of dandelion extract and 0.1-0.5 part of paeonol.

2. The composition according to claim 1, wherein the composition comprises the following components in parts by weight: 2-4 parts of trehalose, 1-3 parts of tartaric acid, 0.4-0.6 part of dandelion extract and 0.1-0.3 part of paeonol.

3. The composition of claim 1 or 2, further comprising 3-5 parts of ginger extract.

4. The composition according to any one of claims 1 to 3, wherein the composition comprises 2 to 4 parts of trehalose, 1 to 3 parts of tartaric acid, 0.1 to 0.3 parts of paeonol, 0.4 to 0.6 parts of dandelion extract and 3 to 5 parts of ginger extract.

5. Use of a composition according to any one of claims 1 to 4 for the preparation of a disinfecting gel.

6. A no-wash disinfecting gel, characterized in that it comprises a composition according to any one of claims 1 to 4;

preferably, the mass percentage of the composition in the disinfection gel is 3% -5%.

7. The antiseptic gel of claim 6, further comprising 0.5% to 1.5% chlorhexidine digluconate, 0.03% to 0.06% triethanolamine, 0.2% to 0.25% carbomer, and 1.9% to 1.95% propylene glycol by weight.

8. The antiseptic gel of claim 6 or 7, further comprising 50% to 66% ethanol, 0.9% to 1.0% glycerol, and 0.9% to 1.0% solubilizer, by mass.

9. A sterilising gel according to any of claims 5 to 7, comprising the following components in mass percent: 3 to 5 percent of bacteriostatic composition, 0.5 to 1.5 percent of chlorhexidine digluconate, 0.03 to 0.06 percent of triethanolamine, 0.2 to 0.25 percent of carbomer, 1.9 to 1.95 percent of propylene glycol, 50 to 66 percent of ethanol, 0.9 to 1.0 percent of glycerol, 0.9 to 1.0 percent of solubilizer and the balance of water;

the composition comprises the following components in parts by weight: 2-4 parts of trehalose, 1-3 parts of tartaric acid, 0.1-0.3 part of paeonol, 0.4-0.6 part of dandelion extract and 3-5 parts of ginger extract.

10. A method of preparing a sterilising gel according to any of claims 6 to 9, comprising the steps of:

(1) weighing carbomer and ethanol in proportion, and fully swelling;

(2) adding triethanolamine into the product swelled in the step (1) in proportion, and stirring into gel;

(3) mixing and dissolving chlorhexidine digluconate, antibacterial composition, solubilizer, glycerol and propylene glycol according to a certain proportion;

(4) and (3) adding the product obtained in the step (3) into the product obtained in the step (2) for dissolving to obtain the disinfection gel.