CN110627571A - Special full-water-soluble microbial agent fertilizer for unmanned aerial vehicle and preparation method and application thereof - Google Patents
Special full-water-soluble microbial agent fertilizer for unmanned aerial vehicle and preparation method and application thereof Download PDFInfo
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- CN110627571A CN110627571A CN201911033985.7A CN201911033985A CN110627571A CN 110627571 A CN110627571 A CN 110627571A CN 201911033985 A CN201911033985 A CN 201911033985A CN 110627571 A CN110627571 A CN 110627571A
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- 235000007079 manganese sulphate Nutrition 0.000 claims description 7
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a special full-water-soluble microbial inoculum fertilizer for an unmanned aerial vehicle, a preparation method and application thereof, and relates to the technical field of microbial fertilizers.
Description
Technical Field
The invention relates to the technical field of microbial fertilizers, in particular to a special full-water-soluble microbial agent fertilizer for an unmanned aerial vehicle, and a preparation method and application thereof.
Background
At present, in an agricultural planting area, due to the fact that high input and high output are pursued for a long time, a grower prefers to apply a nitrogen-phosphorus-potassium macroelement fertilizer, and does not pay attention to supplement of fertilizer ingredients such as trace elements and microbial inoculant fertilizers, so that a series of problems of plant nutrition barrier, excessive growth, plant diseases and insect pests breeding, fruit quality reduction, soil degradation and the like are caused. Meanwhile, the existing fertilizer application mode generally adopts the methods of applying base fertilizer, irrigating roots or deeply applying soil and the like to crops by using granular fertilizer, and the application method is not high in utilization rate of the fertilizer because a transformation process is required in soil, so that a large amount of waste is often caused.
The fertilizer can directly enter crops from leaves, so that the fertilizer has the advantages of high absorption speed and capability of greatly increasing nutrient elements in crops in a short time. However, the spreading performance of the existing foliar spray fertilizers is generally poor, the cuticle of the leaves of the plants is thick, and the leaves cannot sufficiently absorb the fertilizers, so that the systemic conduction effect of the plants is poor, and the fertilizer efficiency cannot be sufficiently exerted. Therefore, although the fertilizing mode of foliar spraying has great advantages compared with other fertilizer application methods, in the field of agricultural planting, the planting effect of the foliar fertilizer after being sprayed in a large area by using the existing foliar fertilizer is not ideal. In addition, the existing foliar spray fertilizers generally do not have insect expelling effects, which are not favorable for meeting the requirements of large-area industrialization and mechanized production in the existing agricultural planting.
Therefore, it is necessary and urgent to research and develop a microbial inoculant fertilizer which has rich nutrient substances, high plant absorption rate, safety, environmental protection, good large-range spraying effect and disease and insect repelling effect, so as to meet the requirements of large-area industrialization and mechanized production in the existing agricultural planting.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a microbial agent fertilizer, which can realize the synergistic effect by compounding (a) a microbial agent and a metabolite thereof, (b) amino acid, (c) trace elements and (d) orange peel essential oil, has the advantages of rich nutrient substances, high plant absorption rate and good safety, and has the effects of expelling insects and preventing diseases and pests due to the addition of the microbial agent, the metabolite thereof and the orange peel essential oil.
The second purpose of the invention is to provide a preparation method of the microbial inoculum fertilizer, which is simple and easy to implement, convenient to operate, free of special processing equipment and quite suitable for industrial large-scale production.
The third purpose of the invention is to provide the application of the microbial inoculant fertilizer, and the microbial inoculant fertilizer can be widely applied to the field of spraying and planting of crop fertilizers.
The invention provides a special full-water-soluble microbial inoculum fertilizer for an unmanned aerial vehicle, which comprises the following components in part by weight:
(a) microbial agents and metabolites thereof;
(b) an amino acid;
(c) trace elements, and
(d) orange peel essential oil.
Further, the microbial agent and the metabolite thereof comprise at least one of photosynthetic bacteria and the metabolite thereof, bacillus licheniformis and the metabolite thereof, bacillus laterosporus and the metabolite thereof, bacillus megaterium and the metabolite thereof, paecilomyces lilacinus and the metabolite thereof, bacillus subtilis and the metabolite thereof, paenibacillus mucilaginosus and the metabolite thereof, bacillus amyloliquefaciens and the metabolite thereof, paenibacillus polymyxa and the metabolite thereof, beauveria bassiana and the metabolite thereof, verticillium pachysarum and the metabolite thereof, bacillus pumilus and the metabolite thereof, and actinomycetes and the metabolite thereof;
preferably, the mass percentage of the microbial agent and the metabolite thereof in the microbial agent fertilizer is 0-35 wt% based on the mass of the microbial agent fertilizer, and 0 is not included;
more preferably, the mass percentage of the microbial agent and the metabolite thereof in the microbial agent fertilizer is 0.001-35 wt% based on the mass of the microbial agent fertilizer;
preferably, the microbial agent and the metabolite thereof are full water-soluble microbial agents and metabolites thereof.
Further, the amino acid comprises at least one of glutamine, lysine, glycine, tryptophan, serine, methionine, asparagine, isoleucine, threonine, proline, arginine, tyrosine, histidine, alanine, cysteine, valine, phenylalanine, and leucine;
preferably, the amino acid accounts for 0-30 wt% of the microbial agent fertilizer based on the mass of the microbial agent fertilizer, and does not comprise 0;
more preferably, the amino acid accounts for 0.001-30 wt% of the microbial agent fertilizer based on the mass of the microbial agent fertilizer.
Further, the trace elements comprise water-soluble inorganic salt preparations or water-soluble metal chelating agents;
preferably, the water-soluble inorganic salt preparation comprises a mixture of ferrous sulfate, zinc sulfate heptahydrate, boric acid, ammonium molybdate and manganese sulfate;
more preferably, the compound mass ratio of the ferrous sulfate, the zinc sulfate heptahydrate, the boric acid, the ammonium molybdate and the manganese sulfate is (1-10): 0.5-10);
preferably, the water-soluble metal chelating agent comprises a mixture of EDTA-Fe, EDTA-Zn, EDTA-B, EDTA-Mn and EDTA-Cu;
more preferably, the compound mass ratio of EDTA-Fe, EDTA-Zn, EDTA-B, EDTA-Mn and EDTA-Cu is (1-10): 0.5-10);
preferably, the trace elements account for 0-10 wt% of the microbial agent fertilizer based on the mass of the microbial agent fertilizer, and 0 is not included;
more preferably, the trace elements account for 0.001-10 wt% of the microbial inoculum fertilizer based on the mass of the microbial inoculum fertilizer.
Further, based on the mass of the microbial agent fertilizer, the orange peel essential oil accounts for 0-10 wt% of the mass of the microbial agent fertilizer, and does not comprise 0;
preferably, the orange peel essential oil accounts for 0.001-10 wt% of the microbial agent fertilizer.
Furthermore, the effective viable count of the microbial inoculum fertilizer is more than or equal to 2.0 hundred million/g;
preferably, the microbial inoculant fertilizer further comprises water.
Further, the microbial inoculum fertilizer comprises the following components in percentage by mass:
(a) 0.001-35 wt% of microbial agent and metabolite thereof;
(b) 0.001-30 wt% of amino acid;
(c) 0.001 to 10 wt% of trace elements, and
(d) 0.001-10 wt% of orange peel essential oil;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
preferably, the microbial inoculum fertilizer comprises the following components in percentage by mass:
(a) 0.001-10 wt% of microbial agent and metabolite thereof;
(b) 0.001-20 wt% of amino acid;
(c) 0.001 to 2 wt% of trace elements, and
(d) 0.001-5 wt% of orange peel essential oil;
the balance of water, and the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%.
The preparation method of the microbial agent fertilizer provided by the invention comprises the following steps:
adding the raw materials in the formula amount into water, and fully dissolving to obtain the microbial agent fertilizer.
Further, the preparation method specifically comprises the following steps:
fully dissolving amino acid (b) and trace elements (c) in a formula amount into partial water to obtain solution A;
dissolving the orange peel essential oil (a) and the microbial agent (d) and metabolites thereof in the rest amount of water to obtain a solution B;
(III) uniformly mixing the solution A obtained in the step (I) with the solution B obtained in the step (II) to obtain a microbial agent fertilizer;
the sequence of step (I) and step (II) can be switched.
The invention provides an application of the microbial inoculum fertilizer in the field of crop fertilizer spraying and planting;
preferably, the crop fertilizer spraying and planting comprises agricultural plant protection unmanned aerial vehicle fertilizer spraying.
Compared with the prior art, the invention has the beneficial effects that:
the microbial agent fertilizer provided by the invention is mainly prepared by compounding (a) a microbial agent and metabolites thereof, (b) amino acid, (c) trace elements and (d) orange peel essential oil, wherein microbial functional bacteria and metabolites thereof in the components can improve plant photosynthesis and promote plant metabolism, and beneficial microorganisms can prevent growth of plant pathogenic bacteria, so that the aim of preventing plant diseases and insect pests is fulfilled; the amino acid can enhance the protein synthesis and sugar content of plants, improve the flavor of agricultural products, improve the disease resistance of crops and reduce the harm of diseases and pests; the trace elements can regulate the growth and development of crops and improve the disease resistance of plants; the essential oil can dissolve the wax layer of plant leaf, stimulate the absorption of plant to various components, and also has anthelmintic effect. The microbial inoculum fertilizer disclosed by the invention can realize a synergistic effect by compounding the components, the prepared microbial inoculum fertilizer is rich in nutrient substances and high in plant absorption rate, all the raw materials are natural and have good extraction safety, and meanwhile, the microbial inoculum, metabolites thereof and orange peel essential oil are added, so that the microbial inoculum fertilizer also has the effects of expelling insects and preventing diseases and pests.
The preparation method of the microbial agent fertilizer provided by the invention comprises the steps of adding the raw materials in the formula amount into water, and fully dissolving to obtain the microbial agent fertilizer. The preparation method is simple and easy to implement, convenient to operate, free of special processing equipment and quite suitable for industrial large-scale production.
The microbial agent fertilizer provided by the invention can be widely applied to the field of crop fertilizer spraying and planting.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
According to one aspect of the invention, a full water-soluble microbial inoculant fertilizer special for unmanned aerial vehicles comprises:
(a) microbial agents and metabolites thereof;
(b) an amino acid;
(c) trace elements, and
(d) orange peel essential oil.
The effects of the components in the microbial agent fertilizer comprise:
microbial agents and metabolites thereof: the microbial agent is added with a microbial functional agent and metabolite thereof which are beneficial to plant growth, can improve plant photosynthesis, promote plant metabolism, accelerate the absorption and transportation of water and nutrients by plants, stimulate crop growth, promote the absorption of various elements by crops and improve the stress resistance of plants. In addition, beneficial microorganisms can prevent the growth of plant pathogenic bacteria, a layer of high-density protective film can be quickly formed on the surface of the plant, so that the plant pathogenic bacteria can not obtain living space, the plant is protected from being damaged by the pathogenic bacteria, meanwhile, the microbial agent can secrete antibacterial substances, and the germination and hypha growth of pathogenic bacteria spores are inhibited, so that the aim of preventing plant diseases and insect pests is fulfilled.
Amino acids: the amino acid is added into the microbial agent, so that the growth of root systems can be enhanced, the synthesis of chlorophyll is increased, the photosynthesis is promoted, the synthesis of auxin is increased, the cell tissue differentiation is participated, the germination is promoted, the plant growth is facilitated, the protein synthesis and sugar content of plants are increased, the flavor of agricultural products is improved, the stomatal opening is adjusted, the germ resistance is realized, the salt stress resistance is improved, the tolerance is improved, and the harm of diseases and insects is reduced.
Trace elements: the addition of the corresponding trace elements is possible for the person skilled in the art according to the known techniques of microbial agents, for example, the above-mentioned adjuvants can be typically, but not exclusively, iron, zinc, boron, manganese and copper.
Orange peel essential oil: the microbial agent is added with the orange peel essential oil, the limonene rich in the orange peel essential oil can dissolve a wax layer existing in plant leaves, the absorption amount of various components of a plant is stimulated, fruit expansion is promoted, the activities of superoxide dismutase and catalase are enhanced to play an antioxidation role, the cell aging is inhibited, the harvesting period of crops is prolonged, and the fruit bearing time is prolonged. In addition, the orange peel essential oil serving as a natural extraction raw material has the advantages of safety and environmental protection, has good effects of preventing drifting and promoting sedimentation, and can improve the absorption rate of various components of the microbial agent on plant leaf surfaces.
The microbial agent fertilizer provided by the invention is mainly prepared by compounding (a) a microbial agent and metabolites thereof, (b) amino acid, (c) trace elements and (d) orange peel essential oil, wherein microbial functional bacteria and metabolites thereof in the components can improve plant photosynthesis and promote plant metabolism, and beneficial microorganisms can prevent growth of plant pathogenic bacteria, so that the aim of preventing plant diseases and insect pests is fulfilled; the amino acid can enhance the protein synthesis and sugar content of plants, improve the flavor of agricultural products, improve the disease resistance of crops and reduce the harm of diseases and pests; the trace elements can regulate the growth and development of crops and improve the disease resistance of plants; the essential oil can dissolve the wax layer of plant leaf, stimulate the absorption of plant to various components, and also has anthelmintic effect. The microbial inoculum fertilizer disclosed by the invention can realize a synergistic effect by compounding the components, the prepared microbial inoculum fertilizer is rich in nutrient substances and high in plant absorption rate, all the raw materials are natural and have good extraction safety, and meanwhile, the microbial inoculum, metabolites thereof and orange peel essential oil are added, so that the microbial inoculum fertilizer also has the effects of expelling insects and preventing diseases and pests.
In a preferred embodiment of the present invention, the microbial agent and its metabolite include at least one of photosynthetic bacteria and its metabolite, bacillus licheniformis and its metabolite, bacillus laterosporus and its metabolite, bacillus megaterium and its metabolite, paecilomyces lilacinus and its metabolite, bacillus subtilis and its metabolite, paenibacillus mucilaginosus and its metabolite, bacillus amyloliquefaciens and its metabolite, paenibacillus polymyxa and its metabolite, beauveria bassiana and its metabolite, verticillium pachyces and its metabolite, bacillus pumilus and its metabolite, actinomycetes and its metabolite;
preferably, the mass percentage of the microbial agent and the metabolite thereof in the microbial agent fertilizer is 0-35 wt% based on the mass of the microbial agent fertilizer, and 0 is not included;
more preferably, the mass percentage of the microbial agent and the metabolite thereof in the microbial agent fertilizer is 0.001-35 wt% based on the mass of the microbial agent fertilizer;
in a preferred embodiment of the invention, the microbial agent and the metabolite thereof are all water-soluble microbial agents and metabolites thereof.
In a preferred embodiment of the present invention, the amino acid comprises at least one of glutamine, lysine, glycine, tryptophan, serine, methionine, asparagine, isoleucine, threonine, proline, arginine, tyrosine, histidine, alanine, cysteine, valine, phenylalanine, leucine;
preferably, the amino acid accounts for 0-30 wt% of the microbial agent fertilizer based on the mass of the microbial agent fertilizer, and does not comprise 0;
more preferably, the amino acid accounts for 0.001-30 wt% of the microbial agent fertilizer based on the mass of the microbial agent fertilizer. Typical but non-limiting preferred embodiments of the above-mentioned amino acids in mass percent in the microbial inoculant fertilizer are: 0.1 wt%, 1 wt%, 3 wt%, 5 wt%, 7 wt%, 10 wt%, 15 wt%, 20 wt%, 23 wt%, 28 wt% and 30 wt%.
In a preferred embodiment of the present invention, the trace elements comprise a water-soluble inorganic salt formulation or a water-soluble metal chelator;
in a preferred embodiment, the water-soluble inorganic salt preparation and the water-soluble metal chelating agent typically, but not exclusively, contain iron, zinc, boron, molybdenum, manganese and copper. Wherein, the iron element participates in the oxidation-reduction reaction and the electron transfer between chlorophyll and protein of plants and the body, participates in the respiration of plants and enhances the disease resistance of the plants; zinc element participates in synthesis of chlorophyll and growth hormone, and CO in photosynthesis2The hydration function of the composition promotes protein metabolism, promotes the development of reproductive organs and improves stress resistance; the boron element participates in the transportation of sugar and the synthesis of semi-fiber and cell wall substances, promotes the elongation and cell division of cells, promotes the establishment and development of reproductive organs, promotes the germination of plant pollen and the elongation of pollen tubes, promotes the synthesis of nucleic acid and protein and the transportation of auxin; the molybdenum element participates in the metabolism of nitrate and the synthesis of organic phosphorus compounds, in the photosynthesis and respiration in vivo and enhances the drought resistance, cold resistance and disease resistance; the manganese element participates in the synthesis of chlorophyll and vitamin C, regulates the enzyme activity, promotes the seed germination and the seedling growth, and improves the disease resistance of plants.
In the above preferred embodiment, the water-soluble inorganic salt formulation comprises a mixture of ferrous sulfate, zinc sulfate heptahydrate, boric acid, ammonium molybdate and manganese sulfate;
preferably, the compound mass ratio of the ferrous sulfate, the zinc sulfate heptahydrate, the boric acid, the ammonium molybdate and the manganese sulfate is (1-10): 0.5-10);
more preferably, the compound mass ratio of the ferrous sulfate, the zinc sulfate heptahydrate, the boric acid, the ammonium molybdate and the manganese sulfate is 5:5:3:3: 3;
in the above preferred embodiment, the water-soluble metal chelating agent comprises a mixture of EDTA-Fe, EDTA-Zn, EDTA-B, EDTA-Mn and EDTA-Cu;
more preferably, the compound mass ratio of EDTA-Fe, EDTA-Zn, EDTA-B, EDTA-Mn and EDTA-Cu is (1-10): 0.5-10);
more preferably, the compound mass ratio of EDTA-Fe, EDTA-Zn, EDTA-B, EDTA-Mn and EDTA-Cu is 5:5:3:3: 3;
in the preferred embodiment, the trace elements account for 0-10 wt% of the microbial inoculum fertilizer based on the mass of the microbial inoculum fertilizer, and do not include 0;
preferably, the trace elements account for 0.001-10 wt% of the microbial inoculum fertilizer based on the mass of the microbial inoculum fertilizer. Typical but non-limiting preferred embodiments of the above-mentioned trace elements in percentage by mass in the microbial inoculant fertilizer are: 0.001 wt%, 0.01 wt%, 0.1 wt%, 1 wt%, 2 wt%, 3 wt%, 4 wt%, 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%, and 10 wt%.
In a preferred embodiment of the invention, the orange peel essential oil accounts for 0-10 wt% of the mass of the microbial agent fertilizer based on the mass of the microbial agent fertilizer, and 0 is not included;
preferably, the orange peel essential oil accounts for 0.001-10 wt% of the microbial agent fertilizer. The typical but non-limiting preferred embodiment of the orange peel essential oil accounting for the mass percent of the microbial agent fertilizer is as follows: 0.001 wt%, 0.01 wt%, 0.1 wt%, 1 wt%, 2 wt%, 3 wt%, 4 wt%, 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%, and 10 wt%.
In a preferred embodiment of the invention, the effective viable count of the microbial inoculant fertilizer is more than or equal to 2.0 hundred million/g;
in a preferred embodiment of the invention, the microbial inoculant fertilizer further comprises water.
In a preferred embodiment of the present invention, the microbial inoculant fertilizer comprises the following components in percentage by mass:
(a) 0.001-35 wt% of microbial agent and metabolite thereof;
(b) 0.001-30 wt% of amino acid;
(c) 0.001 to 10 wt% of trace elements, and
(d) 0.001-10 wt% of orange peel essential oil;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
the above-mentioned "comprising" of the present invention means that it may include other components in addition to the above-mentioned (a), (b), (c), (d) components, which impart different characteristics to the above-mentioned microbial inoculant fertilizer.
The "balance" mentioned above in the present invention means a content remaining in addition to the above-mentioned (a), (b), (c), and (d) components and optionally other components in a total amount of 100% by mass.
Preferably, the microbial inoculum fertilizer comprises the following components in percentage by mass:
(a) 0.001-10 wt% of microbial agent and metabolite thereof;
(b) 0.001-20 wt% of amino acid;
(c) 0.001 to 2 wt% of trace elements, and
(d) 0.001-5 wt% of orange peel essential oil;
the balance of water, and the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%.
According to one aspect of the present invention, a preparation method of the microbial inoculant fertilizer comprises the following steps:
adding the raw materials in the formula amount into water, and fully dissolving to obtain the microbial agent fertilizer.
The preparation method of the microbial agent fertilizer provided by the invention comprises the steps of adding the raw materials in the formula amount into water, and fully dissolving to obtain the microbial agent fertilizer. The preparation method is simple and easy to implement, convenient to operate, free of special processing equipment and quite suitable for industrial large-scale production.
In a preferred embodiment of the present invention, the preparation method specifically comprises the following steps:
fully dissolving amino acid (b) and trace elements (c) in a formula amount into partial water to obtain solution A;
dissolving the orange peel essential oil (a) and the microbial agent (d) and metabolites thereof in the rest amount of water to obtain a solution B;
(III) uniformly mixing the solution A obtained in the step (I) with the solution B obtained in the step (II) to obtain a microbial agent fertilizer;
the sequence of step (I) and step (II) can be switched.
According to one aspect of the invention, the application of the microbial inoculum fertilizer in the field of crop fertilizer spraying and planting is provided;
the microbial agent fertilizer provided by the invention can be widely applied to the field of crop fertilizer spraying and planting.
In a preferred embodiment of the invention, the crop fertilizer spray planting comprises agricultural plant protection unmanned aerial vehicle fertilizer spraying.
The technical solution of the present invention will be further described with reference to examples and comparative examples.
Example 1
The microbial inoculum fertilizer comprises the following components in parts by weight:
(a) 10 wt% of microbial agent and metabolite thereof;
(b) 10 wt% of amino acid;
(c) 0.1 wt% of trace elements, and
(d) 2% of orange peel essential oil;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
the preparation method of the microbial agent fertilizer specifically comprises the following steps:
dividing the formula amount into two parts horizontally;
(II) fully dissolving the amino acid (b) and the trace elements (c) in a formula amount into water to obtain a solution A;
dissolving the orange peel essential oil (a) and the microbial agent (d) and metabolites thereof in the rest water according to the formula ratio to obtain a solution B;
and (IV) uniformly mixing the solution A obtained in the step (II) with the solution B obtained in the step (III) to obtain the microbial agent fertilizer.
Example 2
The microbial inoculum fertilizer comprises the following components in parts by weight:
(a) 20 wt% of microbial agent and metabolite thereof;
(b) 5 wt% of amino acid;
(c) 0.1 wt% of trace elements, and
(d) 1 wt% of orange peel essential oil;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
the preparation method of the microbial inoculum fertilizer is the same as that of the example 1.
Example 3
The microbial inoculum fertilizer comprises the following components in parts by weight:
(a) 5 wt% of microbial agent and metabolite thereof;
(b) 20 wt% of amino acid;
(c) 0.15 wt% of trace elements, and
(d) 2 wt% of orange peel essential oil;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
the preparation method of the microbial inoculum fertilizer is the same as that of the example 1.
Example 4
The microbial inoculum fertilizer comprises the following components in parts by weight:
(a) 2 wt% of microbial agent and metabolite thereof;
(b) 23 wt% of amino acid;
(c) 0.15 wt% of trace elements, and
(d) 2 wt% of orange peel essential oil;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
the preparation method of the microbial inoculum fertilizer is the same as that of the example 1.
Example 5
The microbial inoculum fertilizer comprises the following components in parts by weight:
(a) 2 wt% of microbial agent and metabolite thereof;
(b) 24 wt% of amino acid;
(c) 0.1 wt% of trace elements, and
(d) 1 wt% of orange peel essential oil;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
the preparation method of the microbial inoculum fertilizer is the same as that of the example 1.
Example 6
The microbial inoculum fertilizer comprises the following components in parts by weight:
(a) 1 wt% of microbial agent and metabolite thereof;
(b) 25 wt% of amino acid;
(c) 0.1 wt% of trace elements, and
(d) 1 wt% of orange peel essential oil;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
the preparation method of the microbial inoculum fertilizer is the same as that of the example 1.
Example 7
The microbial inoculum fertilizer comprises the following components in parts by weight:
(a) 1 wt% of microbial agent and metabolite thereof;
(b) 24 wt% of amino acid;
(c) 0.2 wt% of trace elements, and
(d) 2 wt% of orange peel essential oil;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
the preparation method of the microbial inoculum fertilizer is the same as that of the example 1.
Comparative example 1
The microbial inoculum fertilizer comprises the following components in parts by weight:
(b) 15 wt% of amino acid;
(c) 0.2 wt% of trace elements, and
(d) 10 wt% of microbial agent and metabolite thereof;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
the preparation method of the microbial agent fertilizer specifically comprises the following steps:
dividing the formula amount into two parts horizontally;
(II) fully dissolving the amino acid (b) and the trace elements (c) in a formula amount into water to obtain a solution A;
(III) dissolving the microbial inoculum fertilizer and the metabolite thereof in the formula amount (d) in the rest water to obtain a solution B;
and (IV) uniformly mixing the solution A obtained in the step (II) with the solution B obtained in the step (III) to obtain the microbial agent fertilizer.
Comparative example 2
The microbial inoculum fertilizer comprises the following components in parts by weight:
(a) 1 wt% of orange peel essential oil;
(b) 20 wt% of amino acid;
(c) 5 wt% of trace elements;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
the preparation method of the microbial agent fertilizer specifically comprises the following steps:
dividing the formula amount into two parts horizontally;
(II) fully dissolving the amino acid (b) and the trace elements (c) in a formula amount into water to obtain a solution A;
(III) dissolving the orange peel essential oil (a) in the formula amount in the rest water to obtain a solution B;
and (IV) uniformly mixing the solution A obtained in the step (II) with the solution B obtained in the step (III) to obtain the microbial agent fertilizer.
Experimental example 1
The microbial agent fertilizer has the technical effects of rich nutrient substances, high plant absorption rate, good safety of natural extraction of all raw materials and strong insect expelling and preventing effects. The inventors used the microbial inoculant fertilizers prepared in examples 1-7 and comparative examples 1 and 2 to perform fertilizer efficiency tests on rice planting, and the specific method is as follows.
1. Materials and methods
The test is arranged in a certain package bearing field of Changsha city, province, Hunan in 2018 in 5 months. The soil is rice soil, the fertility is middle-grade and high-grade, and the soil fertility is uniform. The soil cultivation layer nutrient comprises the following components: 33.8g/kg of organic matter, 101.5mg/kg of alkaline hydrolysis nitrogen and quick-acting phosphorus (P)2O5)14.2mg/kg, quick acting potassium (K)2O)103.8mg/kg。
Test crop rice, variety: xiang late Long No. 12.
The microbial inoculum fertilizer obtained in the embodiments 1-7 and the comparative examples 1 and 2 of the invention is prepared into an aqueous solution.
1.2 methods
The experiment was performed with 10 treatments, randomized block permutation, 3 repetitions, and a cell area of 5hm 2.
Treatment 1: conventional fertilization and 750mL/hm2 of the fertilizer prepared in the embodiment 1 of the invention are respectively sprayed for 1 time and 2 times in the seedling stage and the grouting stage of rice in a mode of spraying the fertilizer by an agricultural plant protection unmanned aerial vehicle;
and (3) treatment 2: conventional fertilization and 750mL/hm2 of the fertilizer prepared in the embodiment 2 of the invention are respectively sprayed for 1 time and 2 times in the seedling stage and the grouting stage of rice in a mode of spraying the fertilizer by an agricultural plant protection unmanned aerial vehicle;
and (3) treatment: conventional fertilization and 750mL/hm2 of the fertilizer prepared in the embodiment 3 of the invention are respectively sprayed for 1 time and 2 times in the seedling stage and the grouting stage of rice in a mode of spraying the fertilizer by an agricultural plant protection unmanned aerial vehicle;
and (4) treatment: conventional fertilization and 750mL/hm2 of the fertilizer prepared in the embodiment 4 of the invention are respectively sprayed for 1 time and 2 times in the seedling stage and the grouting stage of rice in a mode of spraying the fertilizer by an agricultural plant protection unmanned aerial vehicle;
and (4) treatment 5: conventional fertilization and 750mL/hm2 of the fertilizer prepared in the embodiment 5 of the invention are respectively sprayed for 1 time and 2 times in the seedling stage and the grouting stage of rice in a mode of spraying the fertilizer by an agricultural plant protection unmanned aerial vehicle;
and (6) treatment: conventional fertilization and 750mL/hm2 of the fertilizer prepared in the embodiment 6 of the invention are respectively sprayed for 1 time and 2 times in the seedling stage and the grouting stage of rice in a mode of spraying the fertilizer by an agricultural plant protection unmanned aerial vehicle;
and (7) treatment: conventional fertilization and 750mL/hm2 of the fertilizer prepared in the embodiment 7 of the invention are respectively sprayed for 1 time and 2 times in the seedling stage and the grouting stage of rice in a mode of spraying the fertilizer by an agricultural plant protection unmanned aerial vehicle;
and (4) treatment 8: conventional fertilization plus 750mL/hm2 of the fertilizer prepared in comparative example 1 of the invention, adopts a mode of spraying the fertilizer by an agricultural plant protection unmanned aerial vehicle, and sprays for 1 time respectively in the seedling stage and the grouting stage of rice for 2 times in total;
and (4) treatment: conventional fertilization + fertilizer prepared according to comparative example 2 of the present invention 750mL/hm2Spraying fertilizer by an agricultural plant protection unmanned aerial vehicle for 1 time respectively in a rice seedling stage and a grouting stage and for 2 times in total;
and (4) treatment 10: and (5) applying fertilizers conventionally.
The test was performed on the basis of a local conventional fertilization. The conventional fertilization comprises the following steps: 600kg/hm of potassium sulfate compound fertilizer applied at bottom 15-15-152Additionally applying 80kg/hm of ammonium sulfate in the rice green turning period2。
The test rice was transplanted at 3 months and 30 days. And (3) spraying fertilizer liquid or clear water respectively in the seedling stage and the grouting stage of the rice strictly according to the requirements of the test scheme, wherein harvesting is started in 25 days in 6 months, and harvesting is finished in 2 days in 7 months. And (3) respectively counting the yield by taking the cell as a unit for single harvest in the harvest, wherein the yield is the accumulated yield of multiple harvests from 25 days at 6 months to 2 days at 7 months, and field investigation and seed test are simultaneously carried out in the harvest process. The test is carried out by adopting other management measures like a common field except that fertilizer liquid or clear water is sprayed according to the requirement of a scheme.
Specific yield results are shown in the following table:
finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. The full-water-soluble microbial inoculum fertilizer special for the unmanned aerial vehicle is characterized by comprising the following components in parts by weight:
(a) microbial agents and metabolites thereof;
(b) an amino acid;
(c) trace elements, and
(d) orange peel essential oil.
2. The all-water-soluble microbial inoculant fertilizer special for the unmanned aerial vehicle as claimed in claim 1, wherein the microbial inoculant and its metabolites comprise at least one of photosynthetic bacteria and its metabolites, bacillus licheniformis and its metabolites, bacillus laterosporus and its metabolites, bacillus megaterium and its metabolites, paecilomyces lilacinus and its metabolites, bacillus subtilis and its metabolites, paenibacillus mucilaginosus and its metabolites, bacillus amyloliquefaciens and its metabolites, paenibacillus polymyxa and its metabolites, beauveria bassiana and its metabolites, verticillium pachyspora and its metabolites, bacillus pumilus and its metabolites, actinomycetes and its metabolites;
preferably, the mass percentage of the microbial agent and the metabolite thereof in the microbial agent fertilizer is 0-35 wt% based on the mass of the microbial agent fertilizer, and 0 is not included;
more preferably, the mass percentage of the microbial agent and the metabolite thereof in the microbial agent fertilizer is 0.001-35 wt% based on the mass of the microbial agent fertilizer;
preferably, the microbial agent and the metabolite thereof are full water-soluble microbial agents and metabolites thereof.
3. The all-water-soluble microbial inoculant fertilizer special for unmanned aerial vehicle as claimed in claim 1, wherein said amino acids comprise at least one of glutamine, lysine, glycine, tryptophan, serine, methionine, asparagine, isoleucine, threonine, proline, arginine, tyrosine, histidine, alanine, cysteine, valine, phenylalanine, leucine;
preferably, the amino acid accounts for 0-30 wt% of the microbial agent fertilizer based on the mass of the microbial agent fertilizer, and does not comprise 0;
more preferably, the amino acid accounts for 0.001-30 wt% of the microbial agent fertilizer based on the mass of the microbial agent fertilizer.
4. The all-water-soluble microbial inoculant fertilizer special for unmanned aerial vehicle of claim 1, wherein said trace elements comprise water-soluble inorganic salt preparations or water-soluble metal chelating agents;
preferably, the water-soluble inorganic salt preparation comprises a mixture of ferrous sulfate, zinc sulfate heptahydrate, boric acid, ammonium molybdate and manganese sulfate;
more preferably, the compound mass ratio of the ferrous sulfate, the zinc sulfate heptahydrate, the boric acid, the ammonium molybdate and the manganese sulfate is (1-10): 0.5-10);
preferably, the water-soluble metal chelating agent comprises a mixture of EDTA-Fe, EDTA-Zn, EDTA-B, EDTA-Mn and EDTA-Cu;
more preferably, the compound mass ratio of EDTA-Fe, EDTA-Zn, EDTA-B, EDTA-Mn and EDTA-Cu is (1-10): 0.5-10);
preferably, the trace elements account for 0-10 wt% of the microbial agent fertilizer based on the mass of the microbial agent fertilizer, and 0 is not included;
more preferably, the trace elements account for 0.001-10 wt% of the microbial inoculum fertilizer based on the mass of the microbial inoculum fertilizer.
5. The all-water-soluble microbial inoculant fertilizer special for the unmanned aerial vehicle as claimed in claim 1, wherein the orange peel essential oil accounts for 0-10 wt% of the mass of the microbial inoculant fertilizer, and does not comprise 0;
preferably, the orange peel essential oil accounts for 0.001-10 wt% of the microbial agent fertilizer.
6. The all-water-soluble microbial inoculum fertilizer special for the unmanned aerial vehicle according to any one of claims 1 to 5, wherein the effective viable count of the microbial inoculum fertilizer is more than or equal to 2.0 hundred million/g;
preferably, the microbial inoculant fertilizer further comprises water.
7. The all-water-soluble microbial inoculant fertilizer special for the unmanned aerial vehicle of any one of claims 1 to 5, wherein the microbial inoculant fertilizer comprises the following components in percentage by mass:
(a) 0.001-35 wt% of microbial agent and metabolite thereof;
(b) 0.001-30 wt% of amino acid;
(c) 0.001 to 10 wt% of trace elements, and
(d) 0.001-10 wt% of orange peel essential oil;
the balance of water, wherein the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%;
preferably, the microbial inoculum fertilizer comprises the following components in percentage by mass:
(a) 0.001-10 wt% of microbial agent and metabolite thereof;
(b) 0.001-20 wt% of amino acid;
(c) 0.001 to 2 wt% of trace elements, and
(d) 0.001-5 wt% of orange peel essential oil;
the balance of water, and the sum of the mass percentages of the components in the microbial inoculum fertilizer is 100%.
8. A preparation method of the microbial inoculant fertilizer according to any one of claims 1 to 7, wherein the preparation method comprises the following steps:
adding the raw materials in the formula amount into water, and fully dissolving to obtain the microbial agent fertilizer.
9. The method for preparing a microbial inoculant fertilizer as claimed in claim 8, wherein the method specifically comprises the steps of:
fully dissolving amino acid (b) and trace elements (c) in a formula amount into partial water to obtain solution A;
dissolving the orange peel essential oil (a) and the microbial agent (d) and metabolites thereof in the rest amount of water to obtain a solution B;
(III) uniformly mixing the solution A obtained in the step (I) with the solution B obtained in the step (II) to obtain a microbial agent fertilizer;
the sequence of step (I) and step (II) can be switched.
10. The application of the microbial inoculant fertilizer as claimed in any one of claims 1 to 7 in the field of crop fertilizer spraying and planting;
preferably, the crop fertilizer spraying and planting comprises agricultural plant protection unmanned aerial vehicle fertilizer spraying.
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Application publication date: 20191231 |