CN110623184A - Novel special health-care beverage for middle-aged and old people - Google Patents
Novel special health-care beverage for middle-aged and old people Download PDFInfo
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Abstract
The invention discloses a novel special health-care beverage for middle-aged and elderly people, belonging to the technical field of biology. According to the application, shark cartilage powder, seaweed and scallop are used as raw materials, and a modern bioseparation technology, a biological enzymolysis technology, a mixed microbial fermentation technology and a marine organism resource nutrition blending technology are integrated to develop the calcium-supplementing health drink for the old with a high calcium-supplementing effect, wherein the raw materials comprise 100 parts of shark cartilage powder, 10-20 parts of seaweed and 5-10 parts of scallop in a compatibility ratio; the mixed aerobic fermentation strain mainly comprises bacillus coagulans, bacillus subtilis and saccharomyces cerevisiae; in addition, the health-care beverage for supplementing calcium for the old is rich in a plurality of functional active substances such as aminosugar, glycosaminoglycan, flavone, saponin and the like, has a remarkable immunity enhancing effect, is suitable for middle-aged and old consumer groups, and is a novel efficient health-care beverage for supplementing calcium for the old.
Description
Technical Field
The invention relates to a novel special health-care beverage for middle-aged and elderly people, in particular to a calcium-supplementing health-care beverage for the elderly people with high immune health-care efficacy prepared by synergistic fermentation of microorganisms, belonging to the technical field of biology.
Background
Shark is one of the oldest and longest-lived deep sea marine organisms in the world, has survived for four hundred million years on earth, has a perfect immune system, is extremely strong in vitality, is hardly infected by diseases, can recover wounds quickly even after being subjected to trauma, and has a cancer probability of about 1/million. The shark medicine in China is originally seen in the book Ben Cao Jing Ji Zhu, the cartilage of the shark medicine mainly comprises cartilage cells, collagen fibers, a large amount of proteoglycan and extracellular matrix, and the polysaccharide extracted from the shark cartilage is mainly used for clinically treating neuralgia, migraine, pain after abdominal cavity operation and the like. In developed countries such as Europe, America, Japan, etc., the shark cartilage polysaccharide is used as health food or health medicine for preventing and treating coronary heart disease, angina pectoris, myocardial infarction, etc. for a long time, and has no obvious toxic and side effects. In recent years, researches show that the shark cartilage contains various mucopolysaccharides and chondroitin sulfate besides minerals such as calcium, phosphorus, magnesium and the like, has remarkable anticoagulation, blood fat reduction, antiviral, anti-tumor and other functions, and is an unavailable natural food resource. Because the shark cartilage powder has the natural effects of promoting the regeneration of human cartilage and inhibiting new vessels, the shark cartilage powder is an excellent calcium-supplementing raw material. In recent years, the development of shark fin bone meal health food is rapidly developed, but the defects of single raw material, insufficient utilization of functional active ingredients, inconvenience in eating of the product and the like of the health product still exist, and the sustainable development of the shark fin bone meal health food industry is restricted.
According to the survey of nutriologists, the method comprises the following steps: the calcium taken by the old in China from food generally does not reach the actual required amount (1000-1500 mg each day), so the calcium supplement for the old has great significance for keeping the health. At present, a large number of calcium supplement products for old people exist in the market, but the calcium supplement effect is not ideal due to the fact that the calcium absorption capacity of the old people is poor, and the development of novel special calcium supplement products for the old people is required.
The invention takes shark cartilage powder as a composition basis, combines seaweed, scallop and other raw materials, utilizes compound microbial inoculum and traditional Chinese medicines with health care and treatment effects to carry out compound fermentation, prepares a brand-new senile calcium-supplementing health-care drink with multiple health effects of marine biological resources such as shark cartilage powder, seaweed, scallop and the like, expands the application approaches of marine biological resources such as medium-sized shark cartilage powder, seaweed and the like, not only keeps the weight, but also plays the nutrition and health-care effects thereof, and overcomes the technical defects of low nutrition digestion rate, complex seaweed resource utilization and production process and high production cost caused by the fact that the prior shark cartilage powder is mostly directly eaten.
Disclosure of Invention
In order to overcome the defects of the prior art, the high-efficiency extraction of functional active substances of marine biological resources such as shark cartilage powder and the like and the remarkable improvement of the calcium supplement effect are realized, the application provides a novel special health-care drink for the middle-aged and the elderly, integrates a modern bioseparation technology, a biological enzymolysis technology, a mixed bacteria microbial fermentation technology and a marine biological resource nutrition allocation technology, develops and develops the high-calcium supplement health-care drink for the elderly, meets the application requirements of the fields such as health care, medical treatment, food and the like on the marine biological functional active ingredients, and the process technology has the advantages of simple and high-efficiency fermentation process, good calcium supplement effect of the obtained product, remarkable immunity enhancement effect, low production cost and easiness in industrial scale production.
The invention realizes the technical effects through the following technical scheme:
a novel special health beverage for the middle-aged and the elderly is characterized in that seaweed and scallop are added in the preparation process of the calcium-supplementing health beverage for the elderly, and the compatibility proportion is as follows: 100 parts of shark cartilage powder, 10-20 parts of seaweed and 5-10 parts of scallop.
Preferably, the raw materials are mixed according to the proportion; 100 parts of shark cartilage powder, 15 parts of seaweed and 8 parts of scallop.
The seaweed raw material is subjected to the following pre-extraction treatment: removing impurities from seaweed, crushing, sieving with a 80-150-mesh sieve, adding purified water with the mass ratio of 10 times of that of the seaweed, and soaking for 30min at 40-60 ℃; adding cellulase with the seaweed mass ratio of 2%, extracting for 2min under the assistance of ultrasonic waves with the ultrasonic power of 250-400W, and then standing for more than 30 min.
The seaweed is one or two of herba Zosterae Marinae, Enteromorpha, Sargassum, and Undaria Pinnatifida.
Preferably, the seaweed is a mixture of enteromorpha prolifera and undaria pinnatifida in a mass ratio of 2: 3.
The scallop is prepared by the following steps: cleaning the processed fresh scallop with tap water, cleaning with natural mineral water or purified water, and draining; homogenizing: adding the scallop into a high-speed homogenizer, adding natural mineral water or purified water, and performing high-speed homogenization until the scallop can pass through a circular sieve with the aperture of 2 mm; sterilizing the homogenized scallop through an autoclave, and cooling for later use.
The application provides a novel special health-care beverage for middle-aged and elderly people, which is characterized by being prepared by aerobic fermentation and two-step anaerobic fermentation processes, and the specific processes comprise:
(1) preparing fermentation liquor: mixing the pulverized shark cartilage powder with the pretreated seaweed enzymatic hydrolysate and scallop slurry, adding 10 times of purified water, and adding 0.15-0.20 mass percent (NH) of fermentation liquor4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(2) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(3) aerobic fermentation: adding a compound microbial agent with the mass ratio of 2-3% of the fermentation liquor into the fermentation liquor, and carrying out aerobic fermentation for 6-12 h at the temperature of 28-35 ℃;
(4) anaerobic fermentation: after the aerobic fermentation is finished, adding 1-2% of lactobacillus acidophilus powder, and performing anaerobic fermentation for 8-16 h at the temperature of 28-35 ℃;
(5) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(6) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(7) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the functional product;
(8) compounding: adding 1-1.5 g/L xylitol, 1-2 g/L isomaltose hypgather, 5-10 g/L citric acid, 5-10 g/L calcium lactate and 3-5 g/L sucrose;
(9) membrane filtration: filtering the calcium-supplementing health-care drink for the old by using a microporous membrane with the pore diameter of 0.15 mu m;
(10) and (3) sterile filling: and filling and sealing in an aseptic filling production line to obtain the special calcium supplement beverage for the old.
The compound microbial agent in the step (3) is a mixed bacterium of bacillus coagulans, bacillus subtilis and saccharomyces cerevisiae, and the inoculation amount ratio of the compound microbial agent is 1:2: 1.
The composite microbial inoculum seedsThe preparation method of the liquid comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours under the condition of 28-32 ℃, liquid bacillus coagulans and bacillus subtilis seed liquid are respectively prepared, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL; then mixing the seed solutions of the two kinds of bacillus with the brewing yeast powder according to a proportion.
Further, the supernatant after centrifugation in the step (6) can be concentrated and then used for preparing health products of any dosage form, such as health capsules, health oral liquid and the like.
The invention provides a novel special health-care beverage for middle-aged and elderly people, which is rich in a plurality of functional active substances such as aminosugar, glycosaminoglycan, flavone, saponin and the like, has a remarkable calcium supplement effect for the elderly people, has a remarkable immunity enhancement effect, and is suitable for consumer groups of middle-aged and elderly people.
The invention provides a novel special health-care beverage for middle-aged and elderly people, which has the following remarkable advantages compared with the prior art:
(1) the application scientifically combines the shark cartilage powder, the seaweed and the scallop, and can fully utilize the growth promotion effect of the seaweed, the scallop and the like on microorganisms; the immunity enhancement effect of the calcium-supplementing health-care drink for the old is obviously improved through the synergistic effect among the shark cartilage powder, the seaweed and the scallop, and the experimental result shows that the synergistic effect among the raw materials is the root cause of the unexpected effect obtained by the compound microbial fermentation;
(2) the method utilizes aerobic bacteria (bacillus coagulans, bacillus subtilis and saccharomyces cerevisiae) to jointly ferment marine biological resources to prepare the functional product, fully utilizes the synergistic effect among microorganisms, and improves the extraction rate and the in-vitro oxidation resistance of the calcium supplement health-care beverage for the old; the multi-strain mixed fermentation not only improves the nutritional characteristics of functional products, but also improves the taste of the calcium-supplementing health-care beverage for the old through the fermentation among microorganisms;
(3) the functionality of the calcium-supplementing health-care beverage for the old is obviously improved through aerobic fermentation and two-step anaerobic fermentation, and the technical effect of '1 +1> 2' is achieved;
(4) the invention adopts two-step fermentation of marine biological resource mixed bacteria, remarkably improves the extraction rate of active ingredients and the nutrition and health care efficacy of the product, and the experimental result shows that the product has better calcium supplement effect for the old, and overcomes the technical defect that the old is difficult to absorb with high efficiency in the existing calcium supplement product.
Detailed Description
The activation method of the compound microbial agent in the following examples is prepared by adopting the following processes: under the aseptic condition, LB culture medium is adopted to culture for 24 hours under the condition of 28-32 ℃, liquid bacillus coagulans and bacillus subtilis seed liquid are respectively prepared, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL; then, the seed solutions of two kinds of bacillus are mixed according to the proportion of 1:2, and then adding the saccharomyces cerevisiae powder microbial inoculum according to the proportion.
The seaweed raw materials in the following examples are all pretreated by the following process: removing impurities from seaweed, crushing, sieving with a 80-150-mesh sieve, adding purified water with the mass ratio of 10 times of that of the seaweed, and soaking for 30min at 40-60 ℃; adding cellulase with the seaweed mass ratio of 2%, extracting for 2min under the assistance of ultrasonic waves with the ultrasonic power of 250-400W, and then standing for more than 30 min.
Scallop slurry was prepared by the following process in the following examples: cleaning the processed fresh scallop with tap water, cleaning with natural mineral water or purified water, and draining; homogenizing: adding the scallop into a high-speed homogenizer, adding natural mineral water or purified water, and performing high-speed homogenization until the scallop can pass through a circular sieve with the aperture of 2 mm; sterilizing the homogenized scallop through an autoclave, and cooling for later use.
Example 1
A novel special health beverage for middle-aged and elderly people comprises the following specific process steps:
(1) pretreatment of raw materials: weighing the raw materials according to the proportion of 100 parts of shark cartilage powder, 16 parts of seaweed and 8 parts of scallop, wherein the seaweed is a mixture of enteromorpha and undaria pinnatifida, and the mass ratio of the seaweed to the undaria pinnatifida is 2: 3; respectively pretreating the raw materials;
(2) preparing fermentation liquor: mixing pulverized shark cartilage powder with pretreated Sargassum enzymolysis solution and scallop slurry, and adding10 times of purified water is added, and then 0.15-0.20 percent (NH) of fermentation liquor by mass ratio is added4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(3) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(4) aerobic fermentation: adding a compound microbial agent with the mass ratio of 3% of the fermentation liquor into the fermentation liquor, and carrying out aerobic fermentation for 12 hours at the temperature of 28-35 ℃;
(5) anaerobic fermentation: after the aerobic fermentation is finished, 2% of lactobacillus acidophilus powder is added, and anaerobic fermentation is carried out for 16h at the temperature of 28-35 ℃;
(6) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(7) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(8) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the functional product;
(9) compounding: adding 1.2g/L xylitol, 1.5g/L isomaltose hypgather, 6g/L citric acid, 8g/L calcium lactate and 4g/L sucrose;
(10) membrane filtration: filtering the calcium-supplementing health-care drink for the old by using a microporous membrane with the pore diameter of 0.15 mu m;
(11) and (3) sterile filling: and filling and sealing in an aseptic filling production line to obtain the special calcium supplement beverage for the old.
Example 2
A novel special health beverage for middle-aged and elderly people comprises the following specific process steps:
(1) pretreatment of raw materials: weighing the raw materials according to the proportion of 100 parts of shark cartilage powder, 16 parts of enteromorpha and 8 parts of scallop, and respectively pretreating the raw materials;
(2) preparing fermentation liquor: mixing the pulverized shark cartilage powder with the pretreated seaweed enzymatic hydrolysate and scallop slurry, adding 10 times of purified water, and adding 0.15-0.20 mass percent (NH) of fermentation liquor4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(3) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(4) aerobic fermentation: adding a compound microbial agent with the mass ratio of 3% of the fermentation liquor into the fermentation liquor, and carrying out aerobic fermentation for 12 hours at the temperature of 28-35 ℃;
(5) anaerobic fermentation: after the aerobic fermentation is finished, 2% of lactobacillus acidophilus powder is added, and anaerobic fermentation is carried out for 16h at the temperature of 28-35 ℃;
(6) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(7) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(8) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the functional product;
(9) compounding: adding 1.2g/L xylitol, 1.5g/L isomaltose hypgather, 6g/L citric acid, 8g/L calcium lactate and 4g/L sucrose;
(10) membrane filtration: filtering the calcium-supplementing health-care drink for the old by using a microporous membrane with the pore diameter of 0.15 mu m;
(11) and (3) sterile filling: and filling and sealing in an aseptic filling production line to obtain the special calcium supplement beverage for the old.
Example 3
A novel special health beverage for middle-aged and elderly people comprises the following specific process steps:
(1) pretreatment of raw materials: accurately weighing the raw materials according to the proportion of 100 parts of shark cartilage powder, 16 parts of undaria pinnatifida and 8 parts of scallop; respectively pretreating the raw materials;
(2) preparing fermentation liquor: mixing the pulverized shark cartilage powder with the pretreated seaweed enzymatic hydrolysate and scallop slurry, adding 10 times of purified water, and adding 0.15-0.20 mass percent (NH) of fermentation liquor4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(3) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(4) aerobic fermentation: adding a compound microbial agent with the mass ratio of 3% of the fermentation liquor into the fermentation liquor, and carrying out aerobic fermentation for 12 hours at the temperature of 28-35 ℃;
(5) anaerobic fermentation: after the aerobic fermentation is finished, 2% of lactobacillus acidophilus powder is added, and anaerobic fermentation is carried out for 16h at the temperature of 28-35 ℃;
(6) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(7) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(8) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the functional product;
(9) compounding: adding 1.2g/L xylitol, 1.5g/L isomaltose hypgather, 6g/L citric acid, 8g/L calcium lactate and 4g/L sucrose;
(10) membrane filtration: filtering the calcium-supplementing health-care drink for the old by using a microporous membrane with the pore diameter of 0.15 mu m;
(11) and (3) sterile filling: and filling and sealing in an aseptic filling production line to obtain the special calcium supplement beverage for the old.
Example 4:
a novel special health beverage for middle-aged and elderly people comprises the following specific process steps:
(1) pretreatment of raw materials: accurately weighing the raw materials according to the proportion of 100 parts of shark cartilage powder, 16 parts of seaweed and 8 parts of scallop, wherein the seaweed is a mixture of enteromorpha and undaria pinnatifida, and the mass ratio of the seaweed to the undaria pinnatifida is 3: 2; respectively pretreating the raw materials;
(2) preparing fermentation liquor: mixing the pulverized shark cartilage powder with the pretreated seaweed enzymatic hydrolysate and scallop slurry, adding 10 times of purified water, and adding 0.15-0.20 mass percent (NH) of fermentation liquor4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(3) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(4) aerobic fermentation: adding a compound microbial agent with the mass ratio of 3% of the fermentation liquor into the fermentation liquor, and carrying out aerobic fermentation for 12 hours at the temperature of 28-35 ℃;
(5) anaerobic fermentation: after the aerobic fermentation is finished, 2% of lactobacillus acidophilus powder is added, and anaerobic fermentation is carried out for 16h at the temperature of 28-35 ℃;
(6) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(7) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(8) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the functional product;
(9) compounding: adding 1.2g/L xylitol, 1.5g/L isomaltose hypgather, 6g/L citric acid, 8g/L calcium lactate and 4g/L sucrose;
(10) membrane filtration: filtering the calcium-supplementing health-care drink for the old by using a microporous membrane with the pore diameter of 0.15 mu m;
(11) and (3) sterile filling: and filling and sealing in an aseptic filling production line to obtain the special calcium supplement beverage for the old.
Example 5 Effect of different Process conditions on the Immunopotentiating ability of calcium-supplementing beverage for elderly people
1. Index measuring method
On the basis of feeding normal feed to the aged mice, calcium supplementing drinks with different formulas and preparation processes are supplemented, the dry weight of thighbone, the maximum negative gravity resistance of thighbone and the calcium content of thighbone of the aged mice are compared under different process conditions, and the average value is taken.
Test method and group
The mice are randomly divided into 13 groups, and the test group is administered 100 mg/kg by intragastric administration of calcium-supplementing health beverage for the elderly-1The negative control group was given the same volume of physiological saline, and 15 were administered to each group. The grouping method comprises the following steps: examples 1-4 groups of the calcium-supplementing health-care drink for old people described in examples 1-4 are separately infused in stomach; control 1 group of calcium-supplementing health drinks for elderly people without anaerobic fermentation in gastric lavage example 1; the control 2 groups of the calcium-supplementing health-care beverage for the old without aerobic fermentation in the intragastric administration example 1; the control group 3 was the calcium supplement health drink for the elderly without anaerobic fermentation in gavage example 2; the control group 4 of calcium-supplementing health-care drinks for the old without aerobic fermentation in the intragastric administration example 2; the control group 5 was the calcium supplement health drink for the elderly who had not been subjected to anaerobic fermentation in gastric lavage example 3; the control group 6 was the calcium supplement health drink for the elderly who had not been subjected to aerobic fermentation in gastric lavage example 3; control 7 groups of calcium-supplementing health drinks for elderly people without anaerobic fermentation in gastric lavage example 4; the control group 8 was the calcium supplement health drink for the elderly who had not been subjected to aerobic fermentation in gavage example 4; the test methods were all the same.
Test results
TABLE 1 influence of different process conditions on nutrition and health-care ability of calcium-supplementing health-care beverage for old people
The experiment result of the embodiment 4 shows that the compatibility among the marine biological resource raw materials and the synergistic effect among the fermentation strains have obvious influence on the nutrition and health care performance of the compound calcium-supplementing health-care beverage for the old.
The above embodiments are only used for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; such modifications and substitutions do not depart from the spirit and scope of the present invention as set forth in the appended claims.
Claims (10)
1. A novel special health beverage for the middle-aged and the elderly is characterized in that seaweed and scallop are added in the preparation process of the calcium-supplementing health beverage for the elderly, and the compatibility proportion is as follows: 100 parts of shark cartilage powder, 10-20 parts of seaweed and 5-10 parts of scallop.
2. The novel special health-care beverage for middle-aged and elderly people according to claim 1, wherein the raw materials are mixed in a ratio of; 100 parts of shark cartilage powder, 15 parts of seaweed and 8 parts of scallop.
3. The novel special health drink for middle and old aged people as claimed in claim 1 or 2, characterized in that the seaweed material is subjected to the following pre-extraction treatment: removing impurities from seaweed, crushing, sieving with a 80-150-mesh sieve, adding purified water with the mass ratio of 10 times of that of the seaweed, and soaking for 30min at 40-60 ℃; adding cellulase with the seaweed mass ratio of 2%, extracting for 2min under the assistance of ultrasonic waves with the ultrasonic power of 250-400W, and then standing for more than 30 min.
4. The novel special health beverage for middle-aged and elderly people according to claim 3, wherein the seaweed is one or a mixture of two of kelp, enteromorpha, gulfweed and undaria pinnatifida.
5. The novel special health beverage for middle-aged and elderly people according to claim 3, wherein the seaweed is a mixture of enteromorpha prolifera and undaria pinnatifida in a mass ratio of 2: 3.
6. The novel special health drink for the middle-aged and the elderly as claimed in claim 1, wherein the scallop is prepared by the following steps: cleaning the processed fresh scallop with tap water, cleaning with natural mineral water or purified water, and draining; homogenizing: adding the scallop into a high-speed homogenizer, adding natural mineral water or purified water, and performing high-speed homogenization until the scallop can pass through a circular sieve with the aperture of 2 mm; sterilizing the homogenized scallop through an autoclave, and cooling for later use.
7. The novel special health drink for the middle-aged and the elderly as claimed in claim 1, wherein the calcium-supplementing health drink for the elderly is prepared by aerobic fermentation and two-step anaerobic fermentation processes, and the specific processes comprise:
(1) preparing fermentation liquor: mixing the pulverized shark cartilage powder with the pretreated seaweed enzymatic hydrolysate and scallop slurry, adding 10 times of purified water, and adding 0.15-0.20 mass percent (NH) of fermentation liquor4)2SO4、0.10～0.15% KH2PO4、0.1%MgSO4；
(2) And (3) sterilization: sterilizing at 121 deg.C for 15 min;
(3) aerobic fermentation: adding a compound microbial agent with the mass ratio of 2-3% of the fermentation liquor into the fermentation liquor, and carrying out aerobic fermentation for 6-12 h at the temperature of 28-35 ℃;
(4) anaerobic fermentation: after the aerobic fermentation is finished, adding 1-2% of lactobacillus acidophilus powder, and performing anaerobic fermentation for 8-16 h at the temperature of 28-35 ℃;
(5) and (3) filtering: performing coarse filtration to remove fermentation thalli;
(6) centrifuging: centrifuging at 10000r/min, and separating supernatant;
(7) aging: ageing for 10-20 days at the temperature of 0-10 ℃ in order to promote the stability and clarification of the functional product;
(8) compounding: adding 1-1.5 g/L xylitol, 1-2 g/L isomaltose hypgather, 5-10 g/L citric acid, 5-10 g/L calcium lactate and 3-5 g/L sucrose;
(9) membrane filtration: filtering the calcium-supplementing health-care drink for the old by using a microporous membrane with the pore diameter of 0.15 mu m;
(10) and (3) sterile filling: and filling and sealing in an aseptic filling production line to obtain the special calcium supplement beverage for the old.
8. The novel special health beverage for middle and old aged people as claimed in claim 7, wherein the compound microbial agent in step (3) is a mixture of Bacillus coagulans, Bacillus subtilis and Saccharomyces cerevisiae, and the inoculation amount ratio is 1:2: 1.
9. The novel special health drink for middle and old aged people according to claim 8, wherein the preparation method of the compound microbial inoculant seed liquid comprises the following steps: under the aseptic condition, LB culture medium is adopted to culture for 24 hours under the condition of 28-32 ℃, liquid bacillus coagulans and bacillus subtilis seed liquid are respectively prepared, and the effective viable count of the seed liquid is required to be more than or equal to 109cfu/mL; then mixing the seed solutions of the two kinds of bacillus with the brewing yeast powder according to a proportion.
10. The novel special health beverage for middle-aged and elderly people according to claim 7, wherein the supernatant centrifuged in step (6) can be concentrated for preparing health products of any dosage form.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111955719A (en) * | 2020-07-06 | 2020-11-20 | 齐鲁工业大学 | Special medical food for treating inflammatory bowel disease and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111955719A (en) * | 2020-07-06 | 2020-11-20 | 齐鲁工业大学 | Special medical food for treating inflammatory bowel disease and preparation method thereof |
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