CN110530780A - A kind of cell hyperbaric chamber being equipped on microscopic system - Google Patents
A kind of cell hyperbaric chamber being equipped on microscopic system Download PDFInfo
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- CN110530780A CN110530780A CN201910813452.4A CN201910813452A CN110530780A CN 110530780 A CN110530780 A CN 110530780A CN 201910813452 A CN201910813452 A CN 201910813452A CN 110530780 A CN110530780 A CN 110530780A
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- 230000006835 compression Effects 0.000 claims description 15
- 238000007906 compression Methods 0.000 claims description 15
- 239000011521 glass Substances 0.000 claims description 8
- 238000012544 monitoring process Methods 0.000 claims description 5
- 239000012531 culture fluid Substances 0.000 claims description 3
- 230000008859 change Effects 0.000 abstract description 3
- 238000009434 installation Methods 0.000 abstract description 2
- 230000000007 visual effect Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 16
- 108010066057 cabin-1 Proteins 0.000 description 7
- 230000000694 effects Effects 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000009189 diving Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000001631 hypertensive effect Effects 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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Abstract
本发明涉及一种搭载于显微镜系统的细胞高压舱，其特征在于：包括高压舱、舱体底座、泄压管道和稳定夹持器；通过在显微镜载物台上设置带有可视化结构的高压舱结构，可直接在显微镜载物台上形成细胞的高压环境，直接高压环境中对细胞进行动态观测；避免了从高压环境下取出细胞，引起细胞在高气压条件下变化迅速，导致捕捉到的生理信号是延时的问题；通过在舱体底座上设置稳定夹持器和导向柱结构来压紧高压舱，采用此结构便于快速定位安装和拆卸；在底端盖板上设置导向座，采用导向座结构与导向柱接触有效防止限位导向孔与导向柱对准有偏差的同时，也避免限位导向柱直接与限位导向板的接触实现对底部盖板上限位导向板的有效保护。
The invention relates to a cell hyperbaric chamber mounted on a microscope system, which is characterized in that it includes a hyperbaric chamber, a cabin body base, a pressure relief pipeline and a stable holder; by setting the hyperbaric chamber with a visual structure on the microscope stage The structure can directly form a high-pressure environment for cells on the microscope stage, and directly observe the cells in a high-pressure environment; avoid taking out cells from a high-pressure environment, causing cells to change rapidly under high-pressure conditions, resulting in captured physiological The signal is a matter of time delay; the hyperbaric cabin is compressed by setting a stable holder and a guide column structure on the base of the cabin, which is convenient for quick positioning, installation and disassembly; a guide seat is set on the bottom end cover, and the guide The contact between the seat structure and the guide column effectively prevents the alignment deviation between the limit guide hole and the guide column, and also avoids the direct contact between the limit guide column and the limit guide plate to effectively protect the upper limit guide plate of the bottom cover.
Description
技术领域technical field
本发明涉及高压舱技术领域，尤其涉及一种搭载于显微镜系统的细胞高压舱。The invention relates to the technical field of hyperbaric chambers, in particular to a cell hyperbaric chamber mounted on a microscope system.
背景技术Background technique
近年来，随着国家对海洋开发的日渐重视，潜水活动日益增加，研究高气压对人体生理功能的影响比如惰性气体麻醉、高压神经综合征及高压氧暴露等成为人们关注的重点。但是该领域的研究仍然存在很多技术难题，如我们想观察高气压对培养的细胞和组织的影响，首先需要将培养的细胞或组织置于专用的高气压舱或工作站中进行高气压暴露，待暴露结束且需经过一段时间减压后再取出，再迅速置于显微镜下观察各项指标的变化，由于细胞的某些功能在高气压条件下变化迅速，且这些变化在离开高气压环境后很快消失，因此利用现有方法捕捉到的生理信号是延时的，甚至是错误的，这些缺陷都会对实验产生误导。In recent years, with the country's increasing emphasis on ocean development, diving activities are increasing, and research on the effects of high pressure on human physiological functions, such as inert gas anesthesia, hypertensive neurological syndrome and hyperbaric oxygen exposure, has become the focus of attention. However, there are still many technical difficulties in research in this field. For example, if we want to observe the influence of high pressure on cultured cells and tissues, we first need to place cultured cells or tissues in a dedicated hyperbaric chamber or workstation for high pressure exposure. After the exposure is over, it needs to be taken out after a period of decompression, and then quickly placed under a microscope to observe the changes in various indicators. Because some functions of cells change rapidly under high-pressure conditions, and these changes are very fast after leaving the high-pressure environment. disappears quickly, so the physiological signals captured by existing methods are time-delayed or even wrong, and these defects will mislead the experiment.
目前，并没有这装置能克服上述难题，而利用我们这个可搭载于显微镜载物台并可利用显微镜动态观察的设备就能从根本上解决上述难题，本装置既可以为培养的组织或细胞提供一个深海的压力环境，又可实现在高气压暴露下利用显微镜长时程动态观察培养的组织和细胞的各项指标，提高了科研数据的准确性。At present, there is no such device that can overcome the above-mentioned problems, but using our equipment that can be mounted on the microscope stage and can be dynamically observed by the microscope can fundamentally solve the above-mentioned problems. This device can provide cultured tissues or cells with A deep-sea pressure environment can realize long-term dynamic observation of various indicators of cultured tissues and cells under high-pressure exposure with a microscope, which improves the accuracy of scientific research data.
发明内容Contents of the invention
有鉴于此，本发明的目的在于提供一种搭载于显微镜系统的细胞高压舱，能够提高高压环境下细胞观察的准确性。In view of this, the object of the present invention is to provide a cell hyperbaric chamber mounted on a microscope system, which can improve the accuracy of cell observation in a high pressure environment.
为解决上述技术问题，本发明提供了一种搭载于显微镜系统的细胞高压舱，所述细胞高压舱搭载于显微镜系统的载物台上，其特征在于：包括高压舱、舱体底座、泄压管道和稳定夹持器；In order to solve the above technical problems, the present invention provides a cell hyperbaric chamber mounted on a microscope system, the cell hyperbaric chamber is mounted on the stage of the microscope system, and is characterized in that it includes a hyperbaric chamber, a chamber base, a pressure relief Pipe and stabilizing grippers;
所述高压舱包括高压舱体、顶部盖板、顶部观察窗、底部盖板和底部观察窗；所述高压舱体呈柱体状结构且高压舱体内设有容纳培养液与载玻片的圆柱腔室，所述圆柱腔室将所述高压舱体两端导通；所述高压舱体的侧边上设置有进气口与排气口；所述顶部盖板与底部盖板分别将所述顶部观察窗和所述底部观察窗压紧在高压舱体的两端；The hyperbaric chamber includes a hyperbaric chamber body, a top cover plate, a top observation window, a bottom cover plate and a bottom observation window; the hyperbaric chamber body is a columnar structure and a cylinder for accommodating culture fluid and glass slides is provided in the hyperbaric chamber body. chamber, the cylindrical chamber connects the two ends of the hyperbaric cabin; the side of the hyperbaric cabin is provided with an air inlet and an exhaust port; the top cover and the bottom cover respectively connect the Said top observation window and said bottom observation window are compressed on both ends of the hyperbaric cabin body;
所述舱体底座上开有若干定位孔，所述定位孔通过螺栓将所述舱体底座锁紧在显微镜系统的载物台上跟随载物台移动；所述舱体底座上设置有与高压舱底端配合的导向柱，所述导向柱为阶梯轴结构；所述高压舱的底部盖板上的边缘设置有限位导向板且限位导向板上开有限位导向孔，所述限位导向孔与舱体底座上的导向柱的阶梯面配合实现高压舱的限位导向，所述阶梯轴式的导向柱结构使得舱体底座与高压舱的底端之间形成透光间隙；There are several positioning holes on the base of the cabin, and the positioning holes lock the base of the cabin on the stage of the microscope system through bolts to follow the movement of the stage; The guide column matched with the bottom end of the tank, the guide column is a stepped shaft structure; the edge of the bottom cover plate of the hyperbaric cabin is provided with a limit guide plate and a limit guide hole is opened on the limit guide plate, and the limit guide The hole cooperates with the stepped surface of the guide column on the cabin base to realize the position-limiting guidance of the hyperbaric cabin, and the stepped shaft-type guide column structure forms a light-transmitting gap between the cabin base and the bottom end of the hyperbaric cabin;
所述泄压管道连接在高压舱体的排气口上，所述泄压管道上设置有泄压阀和压力监测表；The pressure relief pipeline is connected to the exhaust port of the high pressure cabin, and the pressure relief pipeline is provided with a pressure relief valve and a pressure monitoring gauge;
所述稳定夹持器设置在舱体底座上且位于高压舱的底端四周压紧在舱体底座上的导向柱阶梯面上。The stable holder is arranged on the base of the cabin body and is located around the bottom of the hyperbaric cabin and pressed against the stepped surface of the guide column on the base of the cabin body.
进一步的，所述稳定夹持器包括固定底座、铰接基座、压紧手柄和压紧块；所述固定底座垂直设置在舱体底座上且分布于高压舱的四周；所述铰接基座设置在固定底座的顶端，所述压紧手柄与压紧块互相垂直设置形成L型结构，所述压紧手柄的一端铰接在铰接基座上，通过转动压紧手柄使压紧块压在限位导向板上。Further, the stable holder includes a fixed base, a hinged base, a compression handle and a compression block; the fixed base is vertically arranged on the cabin base and distributed around the hyperbaric cabin; the hinged base is set On the top of the fixed base, the pressing handle and the pressing block are arranged perpendicular to each other to form an L-shaped structure, one end of the pressing handle is hinged on the hinged base, and the pressing block is pressed to the limit by rotating the pressing handle guide plate.
进一步的，所述限位导向板上的限位导向孔内设置有一限位导向座；所述限位导向座为一对同轴设置的圆柱筒体结构，且限位导向座的其中一圆柱筒体结构嵌入在限位导向孔内，所述限位导向座的另一圆柱筒体结构压在限位导向板的下表面上；所述限位导向柱嵌入在限位导向座内。Further, a limit guide seat is provided in the limit guide hole on the limit guide plate; the limit guide seat is a pair of coaxially arranged cylindrical barrel structures, and one of the cylinders of the limit guide seat The cylinder structure is embedded in the limit guide hole, and the other cylinder structure of the limit guide seat is pressed on the lower surface of the limit guide plate; the limit guide column is embedded in the limit guide seat.
进一步的，所述顶部观察窗与底部观察窗均为蓝镀膜处理的玻璃结构。Further, the top observation window and the bottom observation window are both blue-coated glass structures.
进一步的，所述顶部盖板的边缘与底部盖板的边缘上均设置有螺栓孔，顶部盖板与底部盖板分别通过螺栓锁紧在高压舱体的舱壁上，所述顶部盖板以及底部盖板与高压舱体的端部的接触处均设置有O型密封圈。Further, the edge of the top cover plate and the edge of the bottom cover plate are provided with bolt holes, and the top cover plate and the bottom cover plate are respectively locked on the bulkhead of the hyperbaric cabin by bolts, the top cover plate and O-rings are provided at the contact points between the bottom cover plate and the end of the hyperbaric cabin.
与现有技术相比，本发明具有以下技术效果：Compared with the prior art, the present invention has the following technical effects:
1)本发明中通过在显微镜载物台上设置带有可视化结构的高压舱结构，可直接在显微镜载物台上形成细胞的高压环境，直接在高压环境中对细胞进行动态观测；避免了从高压环境下取出细胞，引起细胞的某些功能在高气压条件下变化迅速，导致捕捉到的生理信号是延时的，甚至是错误的情况。1) In the present invention, by setting a hyperbaric chamber structure with a visualized structure on the microscope stage, a high-pressure environment for cells can be formed directly on the microscope stage, and cells can be dynamically observed directly in the high-pressure environment; Taking out cells under high-pressure environment will cause some functions of cells to change rapidly under high-pressure conditions, resulting in delayed or even wrong situations in the captured physiological signals.
2)本发明中通过在舱体底座上设置稳定夹持器和导向柱结构来压紧高压舱，采用此结构便于快速定位安装和拆卸；在底端盖板上设置导向座，采用导向座结构与导向柱接触有效防止限位导向孔与导向柱对准有偏差的同时，也避免限位导向柱直接与限位导向板的接触实现对底部盖板上限位导向板的有效保护。2) In the present invention, the hyperbaric cabin is compressed by setting a stable gripper and a guide column structure on the cabin base, which is convenient for quick positioning, installation and disassembly; a guide seat is provided on the bottom end cover plate, and a guide seat structure is adopted The contact with the guide column effectively prevents the alignment deviation between the limit guide hole and the guide column, and also avoids the direct contact between the limit guide column and the limit guide plate to effectively protect the upper limit guide plate of the bottom cover.
附图说明Description of drawings
图1为本发明的一种搭载于显微镜系统的细胞高压舱的俯视结构图。FIG. 1 is a top view structure diagram of a cell hyperbaric chamber mounted on a microscope system according to the present invention.
图2为本发明的一种搭载于显微镜系统的细胞高压舱对的侧视结构示意图。Fig. 2 is a side view structural schematic diagram of a cell hyperbaric chamber pair mounted on a microscope system according to the present invention.
附图标记：1.高压舱；11.高压舱体；12.顶部盖板；13.顶部观察窗；14.底部盖板；15.底部观察窗；2.舱体底座；21.导向柱；22.限位导向板；23.限位导向座；3.泄压管道；31.泄压阀；32.压力监测表；4.稳定夹持器；41.固定底座；42.铰接基座；43.压紧手柄；44.压紧块。Reference signs: 1. Hyperbaric cabin; 11. Hyperbaric cabin body; 12. Top cover plate; 13. Top observation window; 14. Bottom cover plate; 15. Bottom observation window; 2. Cabin base; 21. Guide column; 22. Limit guide plate; 23. Limit guide seat; 3. Pressure relief pipe; 31. Pressure relief valve; 32. Pressure monitoring gauge; 4. Stable holder; 41. Fixed base; 42. Hinged base; 43. Compression handle; 44. Compression block.
具体实施方式Detailed ways
为了进一步理解本发明，下面结合实施例对本发明优选实施方案进行描述，但是应当理解，这些描述只是为了进一步说明本发明的特征和优点，而不是对本发明权利要求的限制。In order to further understand the present invention, the preferred embodiments of the present invention are described below in conjunction with the examples, but it should be understood that these descriptions are only to further illustrate the features and advantages of the present invention, rather than limiting the claims of the present invention.
如图1、2所示的一种搭载于显微镜系统的细胞高压舱，该高压舱搭载于显微镜系统的载物台上；包括高压舱1、舱体底座2、泄压管道3和稳定夹持器4。A cell hyperbaric chamber mounted on a microscope system as shown in Figures 1 and 2, the hyperbaric chamber is mounted on the stage of the microscope system; including a hyperbaric chamber 1, a cabin base 2, a pressure relief pipe 3 and a stable clamp device 4.
高压舱1包括高压舱体11、顶部盖板12、顶部观察窗13、底部盖板14和底部观察窗15；高压舱体11呈柱体状结构且高压舱体11内开有容纳培养液与载玻片的圆柱腔室，圆柱腔室将高压舱体11两端导通；高压舱体11的侧边上设置有进气口与排气口；顶部盖板12与底部盖板14分别将顶部观察窗13以及底部观察窗15压紧在高压舱体11的两端；顶部观察窗13与底部观察窗14均为蓝镀膜处理的玻璃结构；顶部盖板12的边缘与底部盖板14的边缘上均设置有螺栓孔，顶部盖板12与底部盖板14分别通过螺栓锁紧在高压舱体11的舱壁上，顶部盖板12以及底部盖板14与高压舱体11的端部的接触处均设置有O型密封圈。Hyperbaric chamber 1 comprises hyperbaric chamber body 11, top cover plate 12, top observation window 13, bottom cover plate 14 and bottom observation window 15; The cylindrical chamber of the glass slide, the cylindrical chamber conducts the two ends of the hyperbaric cabin body 11; the side of the hyperbaric cabin body 11 is provided with an air inlet and an exhaust port; the top cover plate 12 and the bottom cover plate 14 respectively The top observation window 13 and the bottom observation window 15 are pressed tightly on the two ends of the hyperbaric cabin body 11; the top observation window 13 and the bottom observation window 14 are all blue-coated glass structures; the edge of the top cover plate 12 and the bottom cover plate 14 Bolt holes are provided on the edges, and the top cover plate 12 and the bottom cover plate 14 are respectively locked on the bulkhead of the hyperbaric cabin body 11 by bolts. O-rings are provided at the contact points.
舱体底座2上开有若干定位孔，定位孔通过螺栓将舱体底座2锁紧在显微镜系统的载物台上跟随载物台移动；舱体底座2上设置有与高压舱1底端配合的导向柱21，导向柱21为阶梯轴结构；高压舱1的底部盖板14上的边缘设置有限位导向板22且限位导向板22上开有限位导向孔，限位导向孔与舱体底座2上的导向柱21的阶梯面配合实现高压舱1的限位导向，阶梯轴式的导向柱21结构使得舱体底座2与高压舱1的底端之间形成透光间隙；限位导向板22上的限位导向孔内设置有一限位导向座23；限位导向座23为一对同轴设置的圆柱筒体结构，且限位导向座23的其中一圆柱筒体结构嵌入在限位导向孔内，所述限位导向座23的另一圆柱筒体结构压在限位导向板22的下表面上；所述限位导向柱21嵌入在限位导向座23内。A number of positioning holes are opened on the base of the cabin body 2, and the positioning holes lock the base of the cabin body 2 on the stage of the microscope system to follow the movement of the stage through bolts; The guide column 21, the guide column 21 is a stepped shaft structure; the edge on the bottom cover plate 14 of the hyperbaric cabin 1 is provided with a limit guide plate 22 and a limit guide hole is opened on the limit guide plate 22, and the limit guide hole and the cabin body The stepped surface of the guide column 21 on the base 2 cooperates to realize the position-limiting guidance of the hyperbaric cabin 1, and the stepped shaft-type guide column 21 structure forms a light-transmitting gap between the cabin base 2 and the bottom end of the hyperbaric cabin 1; the position-limiting guide A limit guide seat 23 is arranged in the limit guide hole on the plate 22; the limit guide seat 23 is a pair of cylindrical barrel structures coaxially arranged, and one of the cylindrical barrel structures of the limit guide seat 23 is embedded in the limit In the position guide hole, another cylindrical structure of the limit guide seat 23 is pressed against the lower surface of the limit guide plate 22; the limit guide column 21 is embedded in the limit guide seat 23.
泄压管道3连接在高压舱体的排气口上，泄压管道3上设置有泄压阀31和压力监测表32。The pressure relief pipeline 3 is connected to the exhaust port of the high pressure cabin, and the pressure relief pipeline 3 is provided with a pressure relief valve 31 and a pressure monitoring gauge 32 .
稳定夹持器4设置在舱体底座上且位于高压舱1的底端四周压紧在舱体底座2上的导向柱阶梯面上；稳定夹持器4包括固定底座41、铰接基座42、压紧手柄43和压紧块44；固定底座41垂直设置在舱体底座2上且分布于高压舱1的四周；铰接基座42设置在固定底座41的顶端，压紧手柄43与压紧块44互相垂直设置形成L型结构，压紧手柄43的一端铰接在铰接基座42上，通过转动压紧手柄43使压紧块44压在限位导向板22上。Stable holder 4 is arranged on the cabin body base and is positioned at the bottom of hyperbaric cabin 1 and presses on the guide post step surface on cabin body base 2; Compress the handle 43 and the compression block 44; the fixed base 41 is vertically arranged on the cabin base 2 and is distributed around the hyperbaric cabin 1; the hinged base 42 is arranged on the top of the fixed base 41, and the compression handle 43 and the compression block 44 are arranged perpendicular to each other to form an L-shaped structure. One end of the compression handle 43 is hinged on the hinged base 42 , and the compression block 44 is pressed against the position-limiting guide plate 22 by rotating the compression handle 43 .
本发明的工作原理：在高压舱1的将高压舱体11内放入装载有待观测的细胞组织的载玻片及培养液；通过顶部盖板12、顶部观察窗13、底部盖板14和底部观察窗15，将高压舱体密封；将完成密封的高压舱1通过导向柱21安装在舱体底座2上；并通过舱体底座2上的稳定夹持器4将高压舱1压紧在舱体底座2上；然后整体安置与显微镜系统载物台上；打开高压舱体11上的进气口通入气体进行加压，并通过压力监测表32检测高压舱体内部的压力；当达到观测压力时，停止通入气体并进行观测记录相关数据；完成观测后，打开泄压阀31通过高压舱体11上的排气口排出高压舱体11内的气体，当高压舱体11内为常压的情况下打开高压舱体11取出内部载玻片，并收拾相关观测器材，完成整个观测过程。Working principle of the present invention: put into the slide glass and culture fluid that are loaded with the cell tissue to be observed in the hyperbaric cabin body 11 of hyperbaric cabin 1; Through top cover plate 12, top observation window 13, bottom cover plate 14 and bottom The observation window 15 seals the hyperbaric cabin; the sealed hyperbaric cabin 1 is installed on the cabin base 2 through the guide column 21; on the body base 2; then the overall placement on the stage of the microscope system; open the air inlet on the hyperbaric cabin 11 to feed gas for pressurization, and detect the pressure inside the hyperbaric cabin through the pressure monitoring gauge 32; when reaching the observation When the pressure is high, stop feeding the gas and observe and record relevant data; after completing the observation, open the pressure relief valve 31 and discharge the gas in the hyperbaric cabin 11 through the exhaust port on the hyperbaric cabin 11, when the hyperbaric cabin 11 is normal Open the hyperbaric chamber body 11 to take out the internal glass slides under the pressure, and pack up the relevant observation equipment to complete the entire observation process.
本行业的技术人员应该了解，本发明不受上述实施例的限制，上述实施例和说明书中描述的只是说明本发明的原理，在不脱离本发明精神和范围的前提下，本发明还会有各种变化和改进，这些变化和改进都落入要求保护的本发明范围内。本发明要求保护范围由所附的权利要求书及其等效物界定。Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Variations and improvements are possible, which fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.
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