CN110506987B - Composite bidirectional humectant, preparation method and application thereof - Google Patents
Composite bidirectional humectant, preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/302—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances by natural substances obtained from animals or plants
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/36—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances containing a heterocyclic ring
- A24B15/40—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances containing a heterocyclic ring having only oxygen or sulfur as hetero atoms
- A24B15/403—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances containing a heterocyclic ring having only oxygen or sulfur as hetero atoms having only oxygen as hetero atoms
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Abstract
The invention discloses a tobacco composite bidirectional humectant, a preparation method thereof and application of the tobacco composite bidirectional humectant and a moisture-proof agent in tobacco humectant and moisture-proof agent and improvement of smoking quality of cigarettes. The compound two-way humectant is obtained by compounding zymolyte of polygonatum polysaccharide compound cellulase and sucrose fatty acid ester, has synergistic effect, can obviously improve the moisture retention performance and the moisture resistance of tobacco shreds when being added into the tobacco shreds, and can also improve the aftertaste of cigarettes and promote the fragrance and the comfort of the cigarettes.
Description
Technical Field
The invention belongs to the field of tobacco processing, and particularly relates to a composite bidirectional humectant, a preparation method thereof and application thereof in tobacco.
Background
Along with the good situation that the joint recombination of cigarette industry enterprises in China is accelerated and the structure is continuously improved, the cigarette industry enterprises have more urgent needs on high-quality tobacco. Tobacco belongs to capillary porous colloidal substances and shows the characteristics of easy water absorption and water loss. The wet environment of the cigarettes is easy to excessively absorb water, so that the cigarettes are mutually adhered, and the cigarettes have poor combustibility, insufficient aroma and even mildew phenomenon during smoking; and in a dry environment, the cigarettes are easy to lose water, so that the adverse phenomena of breakage, increased smoke irritation and the like are caused. Therefore, how to reduce the environmental sensitivity of the moisture of the cut tobacco and improve the sensory comfort of the cigarettes in the process of sale and storage becomes an important content of the comprehensive treatment of the tobacco and an important link for improving the quality of the cigarettes.
The commonly used humectants in tobacco industry in China are hygroscopic humectants such as glycerol, propylene glycol, sorbitol and the like, and the relative stability of the moisture content of the cut tobacco is maintained through hydrogen bonds formed between hydroxyl groups on the humectants and water molecules. However, these hygroscopic humectants have the disadvantages of lacking moisture barrier effect and undesirable improvement in mouthfeel upon ingestion. The ideal tobacco humectant has two characteristics of moisture retention in a low-humidity environment and moisture resistance in a high-humidity environment, namely, the sensitivity of the moisture content of cigarettes along with the change of the environmental humidity can be reduced, and the moisture content of the cigarettes is always kept in a relatively stable range, namely, bidirectional moisture retention. Recently, some novel hygroscopic humectants such as polysaccharides and natural hydrophilic extracts have been reported at home and abroad, and much attention is paid to the improvement of water retention effect and sensory quality of tobacco shreds in a low-humidity environment, but the moisture-proof effect is not achieved in a high-humidity environment. Many researchers disclose block-type humectants such as paraffin, vegetable oil, etc., and patents for preparing the humectants by compounding the block-type humectants. However, such humectant is generally poor in stability, is easy to cause emulsion breaking in a cut tobacco drying process, cannot form a protective layer on the surface of cut tobacco well to play a role in moisture retention and moisture prevention, and has adverse effects on the taste of cigarettes.
The present invention has been made to solve the above problems.
Disclosure of Invention
The invention aims to provide a composite bidirectional humectant aiming at the defects of the existing humectant, the humectant prepares low molecular weight polysaccharide by carrying out composite cellulase enzymolysis on polygonatum polysaccharide, and the hygroscopicity and the humectant property of cigarettes are improved; meanwhile, the sucrose fatty acid ester is added to improve the moisture resistance of the cigarette, and the two-way moisture retention effect is realized through the synergistic effect of the sucrose fatty acid ester and the sucrose fatty acid ester, and the smoking quality of the cigarette can be improved.
The purpose of the invention is realized by the following technical scheme:
the invention discloses a preparation method of a composite bidirectional humectant, which comprises the following steps:
(1) dissolving rhizoma Polygonati polysaccharide in water to obtain rhizoma Polygonati polysaccharide solution, and adjusting pH and temperature;
(2) adding an enzyme preparation into the polygonatum polysaccharide solution obtained in the step (1) for enzymolysis;
(3) carrying out enzyme deactivation separation on the liquid subjected to the enzyme treatment in the step (2) to obtain polygonatum polysaccharide enzymatic hydrolysate;
(4) and (4) adding sucrose fatty acid ester into the solution obtained in the step (3), and uniformly mixing to obtain the composite bidirectional humectant solution.
The raw materials of sucrose fatty acid ester and natural plant polysaccharide are food grade.
Preferably, the concentration of the polygonatum polysaccharide solution in the step (1) is 2-4 wt%, the pH is 5.0 +/-0.5, and the temperature is 50 +/-0.5 ℃. The activity of the composite cellulase is highest when the pH value is about 5.0 and the temperature is about 50.
Preferably, the enzyme preparation in the step (2) is compound cellulase with the enzyme activity of 4 x 104U/g; the addition amount of the enzyme preparation is 0.1-1.5% of the weight of the polygonatum polysaccharide; the enzymolysis treatment time is 1-3 h. The compound cellulase is too much or too little relative to the addition amount of the polygonatum polysaccharide, and the enzymolysis time is too long or too short, which is not favorable for the moisture retention of the enzymolysis products; the enzymolysis time is too short or the addition amount of enzyme is too little, so that the specific enzymolysis effect cannot be achieved, and the moisturizing property of the enzymolysis product is poor; the enzymolysis time is too long or the addition amount of enzyme is too much, the moisture retention effect of the enzymolysis products on the tobacco is not obviously improved, and even the moisture retention effect is poor. Generally, the tobacco moisture-keeping effect is best by enzymolysis products after 1-3h of enzymolysis.
Preferably, the enzyme deactivation of the step (3) is boiling water bath enzyme deactivation; the separation is centrifugal separation, the centrifugal condition is 4000-.
Preferably, the mixing temperature of the step (4) is 60-80 ℃; the HLB value of the sucrose fatty acid ester is 5, and the structural formula is as follows:
wherein R is a stearic acid group CH3(CH2)16CO-or H, and at least one R is a stearic acid group CH3(CH2)16CO-, the sucrose fatty acid ester is a mixture of corresponding mono-, di-and tri-esters.
The invention also discloses a composite bidirectional humectant prepared by the preparation method.
The third aspect of the invention discloses the application of the composite bidirectional humectant in moisture retention in low-humidity environment and moisture prevention in high-humidity environment of tobacco.
Preferably, the sucrose fatty acid ester accounts for (0.5-2.0) wt% of tobacco, and the polygonatum polysaccharide zymolyte accounts for (0.1-1.0) wt% of tobacco.
More preferably, the sucrose fatty acid ester accounts for 1.0 wt% of the tobacco, and the polygonatum polysaccharide zymolyte accounts for 0.4 wt% of the tobacco.
Preferably, the composite bidirectional humectant is used for moisture retention in a low-humidity environment and moisture prevention in a high-humidity environment of tobacco and improving the smoking quality of cigarettes.
The invention has the beneficial effects that:
1. the composite bidirectional humectant adopts food-grade sucrose fatty acid ester and natural plant polysaccharide as raw materials, has no peculiar smell, is safe and nontoxic, does not generate substances harmful to human bodies during combustion, and has simple preparation and convenient use.
2. The polygahatous polysaccharides zymolyte and sucrose fatty acid ester of the invention are combined together to have synergistic effect, the moisture-keeping and damp-proof effects of the polygahatous polysaccharides zymolyte and sucrose fatty acid ester are superior to those of the traditional moisture-keeping agents such as propylene glycol, and the sensitivity of the cigarette to the environmental humidity can be reduced. The equilibrium moisture content of the tobacco shreds added with 2 wt%/0.4 wt% (sucrose fatty acid ester/polygonatum polysaccharide zymolyte) under the condition of 84% RH (high humidity environment) is reduced by 6.6% compared with propylene glycol, the half-life average moisture absorption rate is reduced to 0.374%/h from 0.384%/h of the propylene glycol, and the comprehensive moisture resistance (moisture resistance index) is improved by 4.9%. Under the condition of 32% RH (low humidity environment), the half-life average desorption rate is reduced from 0.288%/h of propylene glycol to 0.265%/h, and the comprehensive moisture retention performance (moisture retention index) is improved by 2.7%. Therefore, the composite bidirectional humectant can keep moisture under the conditions of high humidity and low humidity.
3. The composite bidirectional humectant disclosed by the invention can be used for realizing moisture retention and moisture prevention of tobacco, improving the sense organ comfort degree of smoke and improving the smoking quality of cigarettes.
4. The research of low-field nuclear magnetic resonance shows that the composite bidirectional humectant can improve the content of chemically bound water of tobacco shreds under a low-humidity condition and the bonding strength of the tobacco shreds with the tobacco shreds, and can reduce the content and the bonding strength of the chemically bound water under a high-humidity condition, so that a bidirectional moisturizing effect, namely a moisturizing effect under a damp-proof and low-humidity condition under the high-humidity condition is realized.
5. In general, enzymatic hydrolysis can expose hydrophilic groups at the ends of the polysaccharide chains, increasing the number of hydrophilic groups per unit mass. Therefore, the polysaccharide can form more hydrogen bonds with water molecules after enzymolysis, so that the content of chemically combined water in the tobacco shreds is increased, and the water stability of the tobacco shreds can be further improved. The invention unexpectedly discovers that the moisturizing performance and the moisture resistance of the moisturizing agent prepared by adding sucrose fatty acid ester into zymolyte which is obtained by enzymolysis of polygonatum polysaccharide by using cellulase, pectinase and the like and compounding the zymolyte with the sucrose fatty acid ester are not improved, and the sensory evaluation effect of the moisturizing agent is poor (see comparative example 2). The results show that the zymolyte of the compound cellulase of the polygonatum polysaccharide and the sucrose fatty acid ester have a synergistic effect after being compounded, so that the polyvidone has the effects of moisture prevention under high-humidity conditions and moisture retention under low-humidity conditions, and the smoking quality of cigarettes can be improved. And the enzymolysis products of other enzymes of the polygonatum polysaccharide and the sucrose fatty acid ester have no synergistic effect after being compounded.
Drawings
FIG. 1 is a graph of the Moisture Protection Index (MPI) and the Moisture Retention Index (MRI) for the composite bi-directional humectant of example 1 and a control.
FIG. 2 is a graph of the Moisture Protection Index (MPI) and the Moisture Retention Index (MRI) for the composite bi-directional humectant of example 2 and the control.
Figure 3 is a graph of the transverse relaxation times (T) at RH-32% equilibrium for different cut tobaccos according to example 42) And (4) distribution.
FIG. 4 is a graph of transverse relaxation times (T) at RH 84% equilibrium for different cut tobaccos of example 42) And (4) distribution.
FIG. 5 shows the moisture resistance index (MPI) and the Moisture Retention Index (MRI) of the cut tobacco of comparative example 1 and the cut tobacco of a control.
FIG. 6 is a graph showing moisture resistance index (MPI) and Moisture Retention Index (MRI) of the cut tobacco of comparative example 2 and the cut tobacco of a control.
In the figure, S-5-H1 represents a compound humectant obtained by compounding sucrose fatty acid ester (HLB is 5) and polygonatum polysaccharide compound cellulase zymolyte; S-5-H0 represents a compound humectant prepared by compounding sucrose fatty acid ester (HLB is 5) and non-enzymatic rhizoma Polygonati polysaccharide.
Detailed Description
For better understanding of the present invention, the following examples are given to further illustrate the present invention, but the present invention is not limited to the following examples.
In the examples, the Moisture Retention Index (MRI) and the moisture resistance index (MPI) are mainly used as evaluation indexes of moisture retention and moisture resistance, and the larger the MRI and the MPI are, the better the moisture retention and moisture resistance are respectively. MRI and MPI were calculated as follows:
and calculating a moisture ratio MR curve according to the moisture absorption and desorption curves of the tobacco shred samples, then performing nonlinear fitting by adopting a Page model, then calculating the half-life average moisture absorption (desorption) rate v as a kinetic index, and calculating to obtain MRI and MPI by taking the equilibrium moisture ratio as a thermodynamic index.
In the formula: mtDry basis water content (%) at time t; m0The initial dry basis water content (%); meThe moisture content (%) on a dry basis at equilibrium; t is time (h); k and n are model constants; t is t1/2Half-life time;
for M of each sampleeAverage value of (d);
the average value of v for each sample.
Example 1
(1) Accurately weighing 2g of rhizoma Polygonati polysaccharide, dissolving in 48g of distilled water to obtain 4 wt% solution, adjusting pH to 5.0, adding compound cellulase to make it 0.1 wt% of rhizoma Polygonati polysaccharide, and performing enzymolysis at 50 deg.C for 2 hr. Inactivating enzyme in boiling water bath, and centrifuging at 8000rpm for 20min to remove precipitate to obtain zymolyte of rhizoma Polygonati polysaccharide compound cellulase;
(2) adding 40g of distilled water into the polygonatum polysaccharide zymolyte, and uniformly mixing;
(3) adding 10g of sucrose fatty acid ester, stirring in a water bath at 80 ℃, and uniformly mixing to obtain the composite bidirectional humectant.
The obtained composite two-way humectant is uniformly sprayed into tobacco shreds, the addition amount of the composite two-way humectant is 2 wt%/0.4 wt% (sucrose fatty acid ester/polygonatum polysaccharide composite cellulose enzymatic hydrolysate) of the tobacco shreds, and the tobacco shreds added with the same amount of propylene glycol (namely 2.4 wt%) are used as a control sample. The cut tobacco is put in a constant temperature and humidity box with the temperature of 22 +/-1) DEG C and the relative humidity of 60 +/-2 percent for balancing for 72h, the moisture resistance and the moisture retention performance are tested under the conditions of 22 ℃, 84 percent RH and 22 ℃ and 32 percent RH respectively to obtain moisture absorption and desorption curves, and the Moisture Retention Index (MRI) and the moisture resistance index (MPI) are calculated. The results obtained are shown in FIG. 1 and in the following table.
Under the condition of 84% RH, the moisture absorption equilibrium moisture content of the cut tobacco added with the composite bidirectional humectant of the embodiment is 34.36% and is lower than that of propylene glycol (36.80%); the half-life moisture absorption rate v is 0.374%/h and is lower than 0.384%/h of propylene glycol; meanwhile, the MPI of the composite bidirectional humectant is higher than that of propylene glycol, as shown in a left column diagram of fig. 1, which shows that the moisture resistance of the cut tobacco is improved in a high-humidity environment after the composite bidirectional humectant is added, and the moisture resistance of the cut tobacco is superior to that of the propylene glycol. Under the condition of 32% RH, the desorption equilibrium moisture content of the cut tobacco added with the composite bidirectional humectant is slightly lower than that of propylene glycol, and the half-life period desorption rate v is 0.265%/h and is lower than 0.288%/h of the propylene glycol; meanwhile, MRI of the composite two-way humectant is higher than that of propylene glycol, as shown in the right histogram of figure 1, which shows that the humectant of the cut tobacco is improved in a low-humidity environment after the composite two-way humectant is added, and the humectant of the cut tobacco is superior to that of propylene glycol. Therefore, the composite two-way humectant of the present example has good two-way moisturizing properties, i.e., moisture resistance under high humidity environment (84% RH) and moisturizing property under low humidity environment (32% RH) are improved.
Example 2
(1) Accurately weighing 2g of polygonatum polysaccharide, dissolving in 48g of distilled water to prepare a solution with the concentration of 4 wt%, adjusting the pH of the solution to 5.0, adding compound cellulase to account for 1.0 wt% of the polygonatum polysaccharide, and performing enzymolysis for 3 hours at the temperature of 50 ℃. Inactivating enzyme in boiling water bath, and centrifuging at 8000rpm for 20min to remove precipitate to obtain rhizoma Polygonati polysaccharide zymolyte;
(2) adding 45g of distilled water into the polygonatum polysaccharide zymolyte;
(3) adding 5g of sucrose fatty acid ester, stirring in a water bath at 80 ℃, and uniformly mixing to obtain 100g of the composite bidirectional humectant.
The composite two-way humectant is sprayed into tobacco shreds, the addition amount of the composite two-way humectant is 1 wt%/0.4 wt% (sucrose fatty acid ester/polygonatum polysaccharide composite cellulase zymolyte) of the tobacco shreds, and the tobacco shreds added with the same amount of propylene glycol (namely 1.4 wt%) are used as a control sample. The cut tobacco is put in a constant temperature and humidity box with the temperature of 22 +/-1) DEG C and the relative humidity of 60 +/-2 percent for balancing for 72h, the moisture resistance and the moisture retention performance are tested under the conditions of 22 ℃, 84 percent RH and 22 ℃ and 32 percent RH respectively to obtain moisture absorption and desorption curves, and the Moisture Retention Index (MRI) and the moisture resistance index (MPI) are calculated. The results obtained are shown in FIG. 2 and the following table.
Under the condition of 84% RH, the half-life moisture absorption rate v of the cut tobacco added with the composite bidirectional humectant is equivalent to that of propylene glycol, but the moisture absorption equilibrium water content is lower than that of the propylene glycol, and the MPI of the composite bidirectional humectant is higher than that of the propylene glycol, as shown in the left column diagram of figure 2, which shows that the moisture resistance of the cut tobacco added with the composite bidirectional humectant is better than that of the cut tobacco added with the propylene glycol. Under the condition of 32% RH, the half-life desorption rate v of the cut tobacco added with the composite bidirectional humectant is 0.219%/h and is lower than 0.248%/h of propylene glycol, and MRI is higher than propylene glycol, which shows that the composite bidirectional humectant has better humectant performance than propylene glycol after being added, as shown in the right histogram of figure 2, therefore, the composite bidirectional humectant disclosed by the invention has good bidirectional humectant performance.
Example 3
Sensory evaluation experiments were carried out on the tobacco shreds in example 2, in which the amount of the composite bidirectional humectant was 1 wt%/0.4 wt% (sucrose fatty acid ester/polygonatum polysaccharide complex cellulase enzymatic hydrolysate) of the tobacco shreds, and the results were as follows, with the tobacco shreds added with the same amount of propylene glycol as a control, with reference to "YCT 138-:
the result shows that the tobacco shred added with the compound humectant is not obviously improved in the aspects of miscellaneous gas and irritation compared with a control sample, but the aroma texture is improved, the smoke is softer and more delicate, the harmony is slightly enhanced, and the aftertaste is cleaner and more comfortable. Compared with the control sample, the whole sensory quality is improved. The compound humectant disclosed by the invention has the effects of high humidity moisture resistance and low humidity moisture retention, and can also improve the sensory comfort of cigarettes and improve the smoking quality of the cigarettes.
Example 4
And analyzing the influence of the composite bidirectional humectant on the existing state and proportion of the tobacco shreds with different water contents by low-field nuclear magnetic resonance.
The amount of the composite bidirectional humectant prepared in reference example 1 was 1 wt%/0.4 wt% (sucrose fatty acid ester/polygonatum polysaccharide complex cellulase enzymatic hydrolysate) of the tobacco shred mass, and the amount of the tobacco shred added with the same amount of propylene glycol was measured by using a low-field nuclear magnetic resonance spectrometer as a control. The experimental parameters were as follows:
CPMG sequence: the main frequency SF is 21MHz, the sampling frequency SW is 100kHz, the offset frequency O1 is 408105.49Hz, the 90 ° pulse time P1 is 18 μ s, the 180 ° pulse time P2 is 36 μ s, the oversampling latency time TW is 2500ms, the number of oversampling times NS is 8, the number of sampling points TD is 37490, the echo time TE is 0.25ms, the number of echoes NECH is 1500, the radio frequency delay RFD is 0.09ms, the digital gain DRG1 is 3, the analog gain RG1 is 20db, and the amplification factor PRG is 2. The results of the experiment are shown in fig. 3 and 4.
In the inverted spectrogram, T2The smaller the value of (a), the more closely the moisture is bound to the tobacco. The peak area size of each peak represents the relative content of different water, and the larger the peak area ratio is, the higher the relative content of the water is. T can be seen for tobacco shreds equilibrated at 84% RH and 32% RH2Three peaks exist in the inversion spectrogram, and the relaxation time is respectively T from small to large21、T22And T23Which means chemically bound water, physically bound water and free water, respectively, the binding force of which is weakened in order.
Tobacco shred T added with S-5-H1 at 32% RH (FIG. 3)21The peak value is smaller than that of propylene glycol, indicating that the water binding capacity is better than that of propylene glycol, and T is simultaneously21The peak area is larger than that of propylene glycol, namely, the proportion of chemically bound water is increased, and the chemically bound water is the water which is most tightly bound and is least easy to dissipate, so that under the low-humidity condition, the addition of S-5-H1 can increase the binding strength of the chemically bound water and the tobacco shreds, improve the content of the chemically bound water, and show the moisture retention performance superior to that of propylene glycol under the low-humidity condition.
As can be seen from FIG. 4, T of the cut tobacco containing S-5-H1 added thereto was found to be 84% RH21The peak is shifted to the right relative to propylene glycol, indicating an increase in the mobility of chemically bound water and binding tightnessDecrease, at the same time, T21The peak area was also lower than propylene glycol, indicating a decrease in the relative amount of chemically bound water. Therefore, S-5-H1 can achieve moisture-proof effect by reducing the interaction strength of tobacco shreds with chemically bound water and reducing the content of chemically bound water under high humidity conditions.
Comparative example 1
The polygonatum polysaccharide composite cellulase zymolyte/sucrose fatty acid ester composite bidirectional humectant is compared with the polygonatum polysaccharide/sucrose fatty acid ester composite in the moisture-keeping and moisture-proof performances of tobacco shreds. Reference example 1 tobacco shreds added with 2 wt%/0.4 wt% (sucrose fatty acid ester/polygonatum polysaccharide complex cellulase substrate) and tobacco shreds added with 2 wt%/0.4 wt% (sucrose fatty acid ester/polygonatum polysaccharide) were prepared, and the tobacco shreds added with the same amount of propylene glycol were used as control samples. Placing in a constant temperature and humidity chamber with temperature (22 + -1) deg.C and relative humidity (60 + -2)% for balancing for 72h, respectively performing moisture resistance test at 22 deg.C and 84% RH and moisture retention test at 22 deg.C and 32% RH to obtain moisture absorption and desorption curves, and calculating Moisture Retention Index (MRI) and moisture resistance index (MPI). The results obtained are shown in FIG. 5 and the following table.
The result shows that under the condition of 32% RH, the polygonatum polysaccharide composite cellulase zymolyte is compounded with sucrose fatty acid ester, and compared with the compound before enzymolysis, the obtained composite bidirectional humectant has the advantages that the equilibrium water content is improved, the half-life desorption rate v is reduced, and MRI is also obviously higher than that before enzymolysis (figure 5), which shows that the moisturizing effect can be obviously improved by compounding the enzymolysis polygonatum polysaccharide with sucrose fatty acid ester. Meanwhile, MPI after enzymolysis is lower than that before enzymolysis, which indicates that the moisture-proof effect is reduced, and is related to that the molecular chain length is shortened after enzymolysis, more hydrophilic groups are exposed, and the hygroscopicity is enhanced, but MPI is still higher than propylene glycol, namely, the moisture-proof effect is better than that of propylene glycol (see figure 5). Therefore, the moisture retention and damp-proof effects of the enzymolyzed compound are better than those of propylene glycol, and the bidirectional moisture retention performance is better.
Comparative example 2
The composite cellulase and pectinase are respectively replaced, a composite humectant is prepared according to the conditions and steps of example 2, and then the obtained composite humectant is sprayed into tobacco shreds, so that the addition amount of the composite humectant is 1 wt%/0.4 wt% (cellulase zymolyte of sucrose fatty acid ester/polygonatum polysaccharide or pectinase zymolyte of sucrose fatty acid ester/polygonatum polysaccharide) of the mass of the tobacco shreds, and the tobacco shreds with the addition amount of 1 wt%/0.4 wt% (sucrose fatty acid ester/polygonatum polysaccharide composite cellulase zymolyte) of the mass of the tobacco shreds and the tobacco shreds with the addition of the same amount of propylene glycol are used as comparison samples. The cut tobacco is put in a constant temperature and humidity box with the temperature of 22 +/-1) DEG C and the relative humidity of 60 +/-2 percent for balancing for 72h, moisture resistance tests are respectively carried out under the conditions of 22 ℃ and 84 percent RH and moisture retention tests are respectively carried out under the conditions of 22 ℃ and 32 percent RH, moisture absorption and desorption curves are obtained, and Moisture Retention Indexes (MRI) and moisture resistance indexes (MPI) are calculated, and the results are shown in the attached figure 6 and the following table.
Under the condition of 84% RH, the moisture absorption equilibrium moisture content of the cut tobacco added with the composite humectant of the comparative example is respectively 36.01% and 35.87%, which is lower than that of propylene glycol (36.41%) but higher than that of the cut tobacco added with zymolyte of sucrose fatty acid ester/polygonatum polysaccharide composite cellulase (35.07%); the half-life moisture absorption rate v is respectively 0.323%/h and 0.312%/h, which are both higher than the zymolyte of the propylene glycol and sucrose fatty acid ester/polygonatum polysaccharide compound cellulase. As shown in the left histogram of fig. 6, the MPI of the composite humectant of the present comparative example are two groups with the lowest relative value, which indicates that the composite humectant of the present comparative example has poor moisture resistance, and the moisture resistance is lower than that of the tobacco shreds added with the zymolyte of the propylene glycol and sucrose fatty acid ester/polygonatum polysaccharide composite cellulase.
Under the condition of 32% RH, the half-life desorption rates v of the composite humectant added in the comparative example are respectively 0.265%/h and 0.258%/h, which are obviously higher than that of the hydrolysate added with 0.248%/h of propylene glycol and 0.219%/h of sucrose fatty acid ester/polygonatum polysaccharide composite cellulase. As shown in the right column diagram of FIG. 6, the MRI of the tobacco shreds added with the composite humectant of the present comparative example is lower than that of the tobacco shreds added with sucrose fatty acid ester/polygonatum polysaccharide composite cellulase and propylene glycol, which shows that the humectant performance of the composite humectant of the present comparative example is not obviously improved. Therefore, in the humectant obtained by compounding zymolyte of the three enzymes of the polygonatum polysaccharide with sucrose fatty acid ester, only the zymolyte of the polygonatum polysaccharide compound cellulase is compounded with the sucrose fatty acid ester to have the bidirectional moisturizing function. The compound humectant disclosed by the invention has the beneficial effects that the enzymolysis product of the compound cellulase of the polygonatum polysaccharide and the sucrose fatty acid ester have a synergistic effect after being compounded, so that the moisturizing performance of the compound humectant disclosed by the invention under a low-humidity condition and the moisture resistance of the compound humectant under a high-humidity condition are improved.
The tobacco rod of comparative example 2 was made into a cigarette for sensory evaluation and compared with other formulations under the same conditions, with the results as follows:
sensory evaluation results of cigarettes show that the humectant added with the compound polygonatum polysaccharide compound cellulase enzymolysis product and sucrose fatty acid ester has the best effect of improving the sensory quality of cigarettes, and the humectant is a compound added with the polygonatum polysaccharide pectinase enzymolysis product, and the compound added with the polygonatum polysaccharide cellulase enzymolysis product is the worst, so the sensory quality of the humectant is even inferior to that of the compound added with the polygonatum polysaccharide. Therefore, the compound of the enzymolysis product of any enzyme of the polygonatum polysaccharide and the sucrose fatty acid ester does not have the effect of improving the sensory evaluation of the tobacco. The sensory quality of the cigarettes further indicates that the zymolyte of the compound cellulase of the polygonatum polysaccharide has a synergistic effect after being compounded with sucrose fatty acid ester.
The above description is only exemplary of the present invention and should not be taken as limiting the invention, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (8)
1. The preparation method of the composite bidirectional humectant is characterized by comprising the following steps:
(1) dissolving rhizoma Polygonati polysaccharide in water to obtain rhizoma Polygonati polysaccharide solution, and adjusting pH and temperature;
(2) adding an enzyme preparation into the polygonatum polysaccharide solution obtained in the step (1) for enzymolysis; the enzyme preparation is composite cellulase with the enzyme activity of 4 multiplied by 104U/g; the addition amount of the enzyme preparation is 0.1-1.5% of the weight of the polygonatum polysaccharide; the enzymolysis treatment time is 1-3 h;
(3) carrying out enzyme deactivation separation on the liquid subjected to the enzyme treatment in the step (2) to obtain a polygonatum polysaccharide zymolyte;
(4) adding sucrose fatty acid ester into the polygonatum polysaccharide zymolyte obtained in the step (3), and uniformly mixing to obtain a composite bidirectional humectant solution; the mixing temperature is 60-80 ℃; the HLB value of the sucrose fatty acid ester is 5, and the structural formula is as follows:
wherein R is a stearic acid group CH3(CH2)16CO-or H, and at least one R is a stearic acid group CH3(CH2)16CO-, the sucrose fatty acid ester is a mixture of corresponding mono-, di-and tri-esters.
2. The preparation method according to claim 1, wherein the concentration of the polygonatum polysaccharide solution in the step (1) is 2-4 wt%, the pH is 5.0 ± 0.5, and the temperature is 50 ± 0.5 ℃.
3. The process according to claim 1, wherein the enzyme deactivation in the step (3) is a boiling water bath enzyme deactivation; the separation is centrifugal separation, the centrifugal condition is 4000-.
4. A composite bidirectional humectant prepared by the preparation method of any one of claims 1 to 3.
5. Use of the composite bi-directional humectant of claim 4 for moisturizing and moisture-proofing in tobacco.
6. The use according to claim 5, wherein the sucrose fatty acid ester is (0.5-2.0) wt% of tobacco, and the polygonatum polysaccharide zymolyte is (0.1-1.0) wt% of tobacco.
7. The use of claim 6, wherein the sucrose fatty acid ester is 1.0 wt% of tobacco and the polygonatum polysaccharide zymolyte is 0.4 wt% of tobacco.
8. Use according to claim 6 or 7, wherein the composite bidirectional humectant is used for tobacco moisture retention and moisture prevention and improvement of smoking quality of cigarettes.
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