CN110499245B - Microalgae in-situ expansion culture system for shellfish culture pond and use method thereof - Google Patents

Abstract

The invention discloses a microalgae in-situ expansion culture system for a shellfish culture pond, which comprises a water lifting device, a nutrient salt supply device, a microalgae expansion device and a seawater in-situ filtering device, wherein the seawater in-situ filtering device is arranged on the seawater in-situ filtering device; the seawater in-situ filtering device comprises a water filtering tank, a water outlet pipeline and a water storage tank, wherein the water filtering tank is filled with water filtering sponge, and one side of the top end of the water filtering tank is provided with a water collecting port; the water lifting device comprises a lower water lifting pipe, a water lifting pump and an upper water lifting pipe which are connected in sequence; the nutrient salt supply device comprises a mother liquor extraction pipe and a mother liquor bottle, and the mother liquor bottle is communicated with the lower water lifting pipe through the mother liquor extraction pipe; the microalgae amplification device comprises a microalgae discharge pipe and a reactor box body, wherein a microalgae liquid overflow port is formed in the side wall of the reactor box. Through the use of the microalgae in-situ expansion culture system, the circulation of in-situ extraction of seawater in the shellfish culture pond, auxiliary injection of nutrient solution, high-order microalgae expansion culture and continuous supply of algae solution is realized, and the continuous supply of bait organisms is increased.

Description

A kind of microalgae in-situ expansion cultivation system for shellfish culture pond and using method thereof

technical field

The invention belongs to the technical field of aquaculture, and in particular relates to a microalgae in-situ expansion cultivation system for shellfish cultivation ponds and a use method thereof.

Background technique

Pond aquaculture is one of the methods of marine aquaculture. Because of its relatively controllable aquaculture environment, it has become more and more popular in recent years. The comprehensive economic benefits of aquaculture have been greatly improved compared with open aquaculture methods. However, due to the differences in the environmental characteristics of various places and the differences in the leading cultured species, the species cultured in ponds in different regions are also not the same. On the eastern coast of my country, the species cultured in open ponds are mainly buried-type shellfish (such as razor clams, Mud cockles, green clams, etc.), in some ponds, there are crustaceans such as portunus trituberculatus and blue crab, and occasionally white shrimp and Penaeus japonicus. These aquaculture ponds mainly increase the output capacity of cultured organisms by replacing natural seawater or by artificial baiting, but both of these two methods have great limitations: First, the method of increasing the output capacity by replacing natural seawater It is obviously affected by the bait in the changed water body. If the bait organisms in the changed water body are not rich, the bait for the shellfish in the cultured water body will be insufficient, the growth will be slow, and the economic benefit will not be high; secondly, increase the output by artificial baiting The method is mostly in ponds where crabs, shrimps and shellfish are mixed, feeding bait for the growth of crabs and shrimps, and residual bait, feces, and naturally reproduced algae for the growth of shellfish. This method will cause aquaculture water. In the case of increasing awareness of environmental protection, this method is not suitable for large-scale promotion, and the bait organisms in this farming method are not rich enough, and the shellfish breeding density should not be too high, which will affect the output.

The bivalve molluscs cultured in ponds are all filter feeders, and they grow by feeding on organic debris and microalgae in the water body. Therefore, in order to improve the environment of shellfish culture ponds, speed up the growth of shellfish or increase the yield of shellfish culture in ponds, in addition to the conventional water change and fertilizer technology, increase the output of ponds through exogenous bait supply Ability is a more scientific approach, so the present invention realizes the purpose of increasing the production capacity of the ponds for cultivation through some exogenous facilities. At present, there are no relevant reports on the in-situ culture of microalgae in culture ponds. Some researchers have developed photobioreactors for microalgae culture, but these photobioreactors have some problems and are not suitable for in-situ culture in shellfish culture ponds. Cultivation of microalgae: first of all, these photobioreactors are small in volume and are only suitable for small-scale microalgae expansion; secondly, the water used for microalgae cultivation is sand-filtered seawater, and water treatment needs to be done in another pool; thirdly, microalgae supply Unsustainable, only intermittent supply, that is, to cultivate a pool of microalgae in a bioreactor, it is necessary to re-clean the equipment and carry out the second cultivation, which is time-consuming and laborious. At the same time, during shellfish breeding, cement ponds are often used to cultivate microalgae, but this cultivation method requires a high degree of facility and cannot be moved, so it is not suitable for in-situ cultivation. The device realizes on-site cultivation of microalgae, realizes in-situ utilization of seawater in a culture pond through the water lifting device, and simultaneously realizes primary cultivation and continuous supply of microalgae.

Contents of the invention

Aiming at the deficiencies of the prior art, the present invention provides a microalgae in-situ expansion system for shellfish culture ponds and its use method. The system uses external kinetic energy to realize the in-situ cultivation of seawater in the culture ponds after simple sedimentation and filtration. Utilization, improve the utilization efficiency of water body, and then realize the natural expansion of microalgae.

To achieve the above object, the technical solution of the present invention is:

A microalgae in-situ cultivation system for a shellfish culture pond, comprising a water lifting device, a nutrient supply device, a microalgae expansion device, and a seawater in-situ filtration device placed in the culture pond; the seawater in-situ filtration device It includes a water filter tank, an outlet pipe and a water storage tank. The water filter tank is filled with a filter sponge, and a water collection port is opened on one side of the top of the filter water tank. The tanks are connected; the water-lifting device includes a lower water-lifting pipe, a water-lifting pump and an upper water-lifting pipe connected in sequence, and the lower end of the lower water-lifting pipe is inserted into the water storage tank; the nutrient supply device includes a mother liquor extraction pipe and a There is a nutrient salt mother liquor and an opaque mother liquor bottle, the mother liquor bottle communicates with the water extraction pipe through the mother liquor extraction pipe; the microalgae expansion device includes an algae discharge pipe and a transparent reactor box for microalgae cultivation , the top of the lifting water pipe penetrates into the reactor box, and the side wall of the reactor box is provided with an algae liquid overflow port for connecting the algae discharge pipe.

Preferably, a sieve baffle is fixedly connected above the water collecting port; a support bar is set up below the sieve baffle to support it.

Preferably, the seawater in-situ filtration device also includes a water retaining plate I and a water retaining plate II; the water retaining plate I is located on the side close to the water collection port, and the top and sides of the water retaining plate I are fixedly connected to The inner surface of the water filter tank, the bottom of the water retaining plate I does not touch the bottom of the water tank; the water retaining plate II is located on the side close to the water storage tank, and the bottom and sides of the water retaining plate II are fixed It is connected to the inner surface of the filter tank, and the top of the water retaining plate II does not touch the top of the water tank.

Preferably, a water lifting protection head made of airstone for water filtration is installed at the bottom of the lower water lifting pipe; a flow rate switch is provided on the water lifting pump.

Preferably, the mother liquor extraction pipe is equipped with a control valve for controlling the flow rate of the nutrient salt mother liquor.

Preferably, the top of the reactor box is provided with a detachable reactor cover; the lower surface of the reactor cover is provided with lighting equipment that provides a light source for the growth of microalgae; the bottom of the reactor box Features nano inflatable tubes.

Preferably, the algae liquid overflow is located on the side away from the water outlet of the lifting pipe, and the height of the algae liquid overflow is lower than the height of the water outlet of the lifting water pipe; the microalgae expansion device also includes a mixing Partition, the mixing partition is installed in the reactor box and divides the reactor box into two parts, the bottom of the mixing partition does not touch the bottom of the reactor box, and the mixing partition Close to the side of the water outlet of the water pipe.

Further, the present invention also provides a method for using a microalgae in-situ expansion system for shellfish culture ponds, including the following specific steps:

(1) According to the differences in the types of microalgae to be cultivated, pre-configure the culture solution required for the expansion of the algae species, and put it in the mother solution bottle for standby;

(2) Pre-expand and cultivate the algae species, and then transfer it into the reactor box,

(3) Place the seawater in-situ filtration device at the bottom of the aquaculture pond. With the help of the natural pressure of seawater, the seawater passes through the initial filtration of the silk screen to block large impurities, and then enters the water filter tank from the water collection port, and the seawater is in the filter sponge. After bypassing and filtering along the bottom of the water retaining plate I and the top of the water retaining plate II, it flows into the water storage tank for standby;

(4) Start the power switch of the water lifting pump, and open the control valve on the mother liquor extraction pipe, pump the seawater in the water storage tank and the nutrient salt mother liquor in the mother liquor bottle into the reactor box, along with the continuous injection of seawater and algae When the liquid level exceeds the overflow port of the algae liquid, the amplified algae liquid is injected into the shellfish culture pond through the algae discharge pipe to feed the shellfish.

Preferably, in step (2), when initially expanding the algae species, the liquid level of the algae species is 1/5 of the height of the reactor box.

Preferably, in step (4), the flow rate switch on the water pump is adjusted to control the amount of water entering the reactor casing; lighting equipment can be selectively turned on at night or on cloudy days to increase the light source required for microalgae reproduction.

Compared with prior art, the advantages of the present invention:

(1) The microalgae in-situ expansion system of the present invention can use external kinetic energy to realize the in-situ utilization of seawater in the culture pond after simple sedimentation and filtration, improve the utilization efficiency of the water body, and then realize the natural expansion of microalgae.

(2) The microalgae in-situ expansion cultivation system of the present invention can realize on-site in-situ cultivation of microalgae, utilize the characteristics of self-expansion of microalgae, and realize the uninterrupted supply of microalgae by continuous injection of water body, which can effectively It can improve the actual effect of microalgae cultivation and provide bait for filter-feeding shellfish cultured in ponds. It has the advantages of simple use, fast expansion of microalgae cultivation, and sufficient supply of bait organisms.

(3) The microalgae in situ expansion cultivation system of the present invention is placed beside the dam of the shellfish culture pond and properly fixed. The system can be deployed as an independent unit, or multiple groups of microalgae in situ expansion can be used. The system is deployed at the same time; and the size of the microalgae in-situ expansion system of the present invention (such as the size of the filter tank, water storage tank, mother liquor bottle, reactor box, etc.) can be adjusted appropriately, so the number of deployment can also be adjusted according to the water body. The concentration of medium bait and shellfish breeding density can be freely selected. After use, the breeding environment and breeding ecology of the pond can be effectively improved.

In summary, through the use of the microalgae in-situ expansion cultivation system of the present invention, the in-situ extraction of seawater in shellfish culture ponds, the auxiliary injection of nutrient solution, the expansion of high-level microalgae, and the continuous supply of algae liquid have been realized. A constant supply of bait organisms.

Description of drawings

The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

Fig. 1 is the in situ expansion culture system of microalgae in the shellfish culture pond of the present invention;

Reference signs: 1, sieve silk retaining net; 2, filter water tank; 3, filter water sponge; 4, water retaining plate 1; 5, water storage tank; 6, lift water protection head; 7, lift water pipe down; Mother liquor bottle; 9. Mother liquor extraction pipe; 10. Control valve; 11. Water pump; 12. Mixing partition; 13. Lifting water pipe; 14. Water outlet; 15. Reactor cover; 16. Algae liquid overflow ; 17, the reactor box; 18, the nano-inflatable tube; 19, the outlet pipe; 20, the water collection port; 21, the strut;

Detailed ways

The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

Example 1

As shown in Figure 1, a microalgae in-situ expansion system for shellfish culture ponds includes a seawater in-situ filtration device, a water extraction device, a nutrient salt supply device and a microalgae expansion device. The seawater in-situ filtration device is placed in the shellfish culture pond, and the water-lifting device is connected to the seawater in-situ filtration device by controlling the length of the water-lifting device (that is, the lower water-lifting pipe), and the nutrient supply device is installed on the water-lifting device , and then put them together on the microalgae expansion device and fix them. Each part of the system is a detachable structure, and the components are connected together by screws or fasteners. In the following, the components of each device will be described one by one.

(1) Seawater in-situ filtration device

Water filter tank (2): used for filtering the seawater in the pond, with the assistance of the filter sponge, it can store water and filter. The top of the water filter box (2) is provided with a water collection port (20), and the water collection port (20) enters the entrance of the water filter box (2) for the seawater in the culture pond, and the opposite side of the bottom end of the water filter box (2) is provided with a The filter tank water outlet (not shown in the figure) that water tank (5) is connected to each other, the setting of water collection port (20) and filter tank (2) outlet position is convenient to prolong the distance of seawater filtration, improves the effect of seawater filtration.

Sieve silk retaining net (1): it is a cover net made of sieve silk mesh, which covers the water collection port (20) at the top of the water filter tank (2), and is used to prevent objects such as small and medium-sized organisms and garbage from entering the filter water tank (2) ), play the role of blocking and primary filtering.

Strut bar (21): be positioned at the central below of sieve silk retaining net (1), be used for sieve silk retaining net (1) prop up, to increase the area of primary filtration.

Water filter sponge (3): filled in the water filter tank (2), multi-layer water filter sponges (3) are combined to play the role of filtering the water body, reducing particulate matter in the water body from entering the microalgae amplification device.

Water retaining plate: divided into water retaining plate I (4) and water retaining plate II (22). The water retaining plate I (4) is located on the side close to the water collecting port (20), and the top and side edges of the water retaining plate I (4) are fixedly connected to the inner surface of the filter tank (2). The bottom of the plate I (4) is a distance away from the bottom of the filter tank (2); The bottom end and the sides are fixedly connected to the inner surface of the water filter box (2), and the top of the water retaining plate II (22) is a distance away from the top of the water filter box (2). The setting of water retaining plate I (4) and water retaining plate II (22) makes the seawater that enters the water filter tank (2) from the water collection port (20) pass along the water retaining plate I (4) in the water filter sponge (3) The bottom and the top of the water retaining plate II (22) go around, which increases the distance of seawater filtration and improves the water filtration performance of the water filter tank (2).

Water storage tank (5): at the bottom of the filter tank (2), it is used to temporarily store the seawater filtered by the filter tank (2), and the natural filtration of the seawater can be realized by the natural pressure of the seawater.

Water outlet pipe (19): a short and thick pipe installed at the water outlet of the water filter tank (2) for communicating with the water filter tank (2) and the water storage tank (5).

(2) Water lifting device

Water lifting pump (11): fixedly installed on the outer wall of the reactor box (17), its operation is used to transport the filtered seawater of the seawater in-situ filtration device and the nutrient salt mother liquor of the nutrient salt supply device to the microalgae expansion device. The water lift pump (11) is equipped with a power switch (not shown) and a flow rate switch (24) for controlling the flow rate of the water body.

Lifting the water pipe (7): its top is connected with the water lifting pump (11), and the lower end is inserted into the water storage tank (5).

Water lifting protection head (6): similar to an air-filled air stone structure, installed at the bottom of the lower water lifting pipe (7) to protect the lower water lifting pipe (7) and prevent particles from entering the microalgae amplification device.

Lifting water pipe (13): its bottom is connected with the water lifting pump (11), and the top is inserted into the reactor box (17) of the microalgae amplification device to transport filtered seawater and nutrient salt mother liquor.

(3) Nutrient salt supply device

Mother liquor bottle (8): It is made of light-shielding and opaque material, and is used to store the nutrient salt mother liquor required for algae expansion.

Mother liquor extraction pipe (9): used to connect the mother liquor bottle (8) and the water extraction pipe (7).

Control valve (10): installed on the mother liquor extraction pipe (9), through the opening/closing state of the control valve (10), the control valve (10) used to control the flow rate of the nutrient salt mother liquor (minimum flow rate is zero, maximum flow rate is Mother liquor extraction pipe (9) full-through state).

The nutrient salt supply device can be hung behind the water lifting device, and then placed on the microalgae expansion device as a whole and fixed.

(4) Microalgae expansion device

Reactor box (17): a reactor used for algae amplification and cultivation. The material is a transparent glass plate to ensure transparency, light absorption and other functions. The top of the lifting water pipe (13) is inserted into the reactor box (17). Filtered seawater and nutrient salt mother liquor flow into the reactor tank (17) from the water outlet (14) at the top of the lifting water pipe (13).

Algae liquid overflow port (16): set on the side wall of the reactor box and located on the side of the water outlet (14) away from the upper lifting pipe (13), the height of the algae liquid overflow port (16) is lower than that of the upper lifting pipe (13). The height of the water outlet (14) of the water pipe (13) is convenient for the water level in the reactor box (17) to rise naturally, and the cultivated algae are discharged through the algae liquid overflow port (16).

Algae discharge pipe (23): installed at the algae liquid overflow outlet (16), the high-density algae liquid that is convenient to discharge directly enters the culture pond through the algae discharge pipe (23).

Mixing partition (12): installed in the reactor box (17), placed at 1/10 of one side of the box, and close to the water outlet (14) of the upper lifting pipe (13), the mixing partition The bottom of (12) is a height away from the bottom of the reactor box (17), and the inside of the reactor box (17) is divided into two parts. After filtering, seawater and nutrient salt mother liquor pass through the After the water outlet (14) of the upper water pipe (13) is pumped into the reactor box (17), under the action of the mixing partition (12), an upward flow is formed in the reactor box (17), and the mixing Filter water and algae-phase water.

Reactor upper cover (15): located on the top of the reactor box (17), it can be opened and closed freely, which is convenient for cleaning and other operations inside the reactor box (17).

Lighting equipment (not shown in the figure): it is installed on the lower surface of the reactor loam cake (15) to provide a light source for the growth and reproduction of the algae liquid in the reactor box (17), which is convenient for night or cloudy time operations, so as to Increase the ability to increase the expansion of microalgae.

Nano inflatable tube (18): laid on the bottom of the reactor box (17), to ensure the supply of gas in algae cultivation.

Each component of the microalgae in-situ expansion system of the present invention can be disassembled, that is, each component constituting the system can be transported separately and then assembled. The present invention can be separately assembled into a seawater in-situ filtration device, a water extraction device, a nutrient salt supply device and a microalgae expansion device, and then these four sub-devices are installed together to form a complete set of microalgae in-situ expansion devices. training system.

The microalgae in-situ expansion cultivation system of the present invention is placed beside the dam of the shellfish culture pond and properly fixed. The microalgae in situ expansion cultivation system of the present invention can be deployed as an independent unit, or multiple groups of microalgae in situ expansion cultivation systems can be deployed simultaneously. Because the size of the microalgae in situ expansion cultivation system of the present invention (such as the size of filter water tank, water storage tank, mother liquor bottle, reactor casing, etc.) can be adjusted appropriately, so the number of deployment can also be based on the concentration of bait in the water body, Free choice of shellfish breeding density, etc.

Example 2

A method for using a microalgae in-situ expansion cultivation system for shellfish culture ponds, mainly divided into two parts: experiment preparation and specific use of the microalgae in-situ expansion cultivation system.

Experiment preparation:

(1) Configuration of nutrient salt mother solution: According to the difference of the types of microalgae to be cultivated, the nutrient salt mother solution required for the expansion of algae species is pre-configured in the laboratory, and then stored in the mother solution bottle for later use.

(2) Expansion of algae species: Under plastic barrels or laboratory conditions, pre-expand the algae species, and then transfer them into the reactor box. The initial liquid level of the algae species is appropriately adjusted according to the concentration of the species to be expanded. Generally, it is advisable to account for more than 1/5 of the volume of the reactor box.

(3) System assembly: the components of the microalgae in situ expansion cultivation system in Example 1 were assembled into a whole according to the connection relationship.

The specific use of the microalgae in situ expansion system includes the following steps:

(1) Place the seawater in-situ filtration device at the bottom of the shellfish culture pond. With the help of the natural pressure of seawater, the seawater passes through the sieve screen (1) to block large impurities, and then enters the filter tank from the water collection port, and the seawater In the water filter sponge, after going around the bottom of the water retaining plate I and the top of the water retaining plate II for secondary filtration, it flows into the water storage tank for subsequent use.

(2) Start the power switch of the water pump, and open the control valve on the mother liquor extraction pipe, and pump the seawater in the water storage tank and the nutrient salt mother liquor in the mother liquor bottle into the reactor box.

(3) According to the weather conditions and the condition of the microalgae in the reactor box, adjust the flow rate switch on the pump to control the inflow of water into the reactor box; you can selectively turn on the lighting equipment at night or on cloudy days to increase microalgae. The light source needed for algal reproduction.

(4) With the continuous injection of seawater and the expansion of algae, when the liquid level exceeds the overflow port of the algae liquid, the expanded algae liquid is injected into the shellfish culture pond through the algae discharge pipe (23) to feed the shellfish.

Through the above steps, the circulation system of in-situ extraction of seawater in the shellfish culture pond, auxiliary injection of nutrient solution, expansion of high-level microalgae and continuous supply of algae solution can be realized, and the continuous supply of bait organisms can be increased.

Although the present invention has been described in detail with general descriptions and specific examples above, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, the modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the protection scope of the present invention.

Claims (4)

1. A method for in-situ culturing microalgae by using a microalgae in-situ expansion culture system, which is characterized by comprising the following steps:

1) According to the difference of the microalgae species to be cultivated, preparing a culture solution required by the expansion cultivation of the microalgae species in advance, and placing the culture solution in a mother liquor bottle for standby;

2) The algae seeds are spread in advance and then transferred into a reactor box body,

3) The seawater in-situ filtering device is placed at the bottom of the aquaculture pond, and after the seawater is filtered for the first time by the bolting silk blocking net to block large-scale impurities by means of the natural pressure of the seawater, the seawater enters the water filtering tank from the water collecting port, and flows into the water storage tank for standby after bypassing and filtering along the bottom end and the top end of the water baffle I and the water baffle II in the water filtering sponge;

4) Starting a power switch of the water lifting pump, opening a control valve on a mother liquor extraction pipe, pumping seawater in a water storage tank and nutrient salt mother liquor in a mother liquor bottle into a reactor box, and injecting amplified algae liquid into a shellfish culture pond through an algae discharging pipe to feed shellfish when the liquid level exceeds an algae liquid overflow port along with continuous injection of seawater and amplification of algae;

the microalgae in-situ expansion culture system comprises a water lifting device, a nutrient salt supply device, a microalgae expansion device and a seawater in-situ filtering device arranged in the culture pond; the seawater in-situ filtering device comprises a water filtering tank, a water outlet pipeline and a water storage tank, wherein the water filtering tank is filled with water filtering sponge, a water collecting port is formed in one side of the top end of the water filtering tank, and the opposite side of the bottom end of the water filtering tank is communicated with the water storage tank through the water outlet pipeline; the water lifting device comprises a lower water lifting pipe, a water lifting pump and an upper water lifting pipe which are connected in sequence, and the lower end of the lower water lifting pipe is inserted into the water storage tank; the nutrient salt supply device comprises a mother liquor extraction pipe and a light-tight mother liquor bottle filled with nutrient salt mother liquor, and the mother liquor bottle is communicated with the lower water lifting pipe through the mother liquor extraction pipe; the microalgae amplification device comprises a microalgae discharge pipe and a transparent reactor box body for microalgae culture, wherein the top end of the upper lifting pipe stretches into the reactor box body, and the side wall of the reactor box is provided with a microalgae liquid overflow port for connecting the microalgae discharge pipe;

a bolting silk blocking net is fixedly connected above the water collecting port; a supporting rod for supporting the bolting silk baffle net is arranged below the bolting silk baffle net;

the seawater in-situ filtering device also comprises a water baffle I and a water baffle II; the water baffle I is positioned at one side close to the water collecting port, the top end and the side edge of the water baffle I are fixedly connected to the inner surface of the water filtering tank, and the bottom end of the water baffle I is not contacted with the bottom end of the water filtering tank; the water baffle II is positioned at one side close to the water storage tank, the bottom end and the side edge of the water baffle II are fixedly connected to the inner surface of the water filtering tank, and the top end of the water baffle II is not contacted with the top end of the water tank;

a control valve for controlling the flow rate of the nutrient salt mother solution is arranged on the mother solution extraction pipe;

the algae liquid overflow outlet is positioned at one side far away from the water outlet of the upper water lifting pipe, and the height of the algae liquid overflow outlet is lower than that of the water outlet of the upper water lifting pipe; the microalgae amplification device further comprises a mixing baffle plate, wherein the mixing baffle plate is arranged in the reactor box body and divides the reactor box body into two parts, the bottom end of the mixing baffle plate is not contacted with the bottom of the reactor box body, and the mixing baffle plate is close to one side of a water outlet of the water lifting pipe.

2. The method according to claim 1, wherein the bottom end of the lower water lifting pipe is provided with a water lifting protection head for water filtering made of gas stone; the water lifting pump is provided with a flow rate switch.

3. The method of claim 1, wherein the top end of the reactor housing is provided with a removable reactor top cover; the lower surface of the reactor upper cover is provided with illumination equipment for providing a light source for microalgae growth; the bottom in the reactor box body is provided with a nano-gas-filled tube.

4. The method according to claim 1, wherein in the step 2), the initial expansion of the algal species is performed, and the liquid level of the algal species is 1/5 of the height of the reactor tank.

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