CN110494547A - System and method for growing algae - Google Patents
System and method for growing algae Download PDFInfo
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- CN110494547A CN110494547A CN201880017301.1A CN201880017301A CN110494547A CN 110494547 A CN110494547 A CN 110494547A CN 201880017301 A CN201880017301 A CN 201880017301A CN 110494547 A CN110494547 A CN 110494547A
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title description 13
- 238000005286 illumination Methods 0.000 claims abstract description 31
- 238000005507 spraying Methods 0.000 claims abstract description 22
- 230000004907 flux Effects 0.000 claims abstract description 10
- 239000012530 fluid Substances 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 5
- 241001501873 Isochrysis galbana Species 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000005259 measurement Methods 0.000 claims description 3
- 230000004888 barrier function Effects 0.000 description 11
- 229910002092 carbon dioxide Inorganic materials 0.000 description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000007789 gas Substances 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 3
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 description 3
- 229960005375 lutein Drugs 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 description 3
- 235000008210 xanthophylls Nutrition 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 240000009108 Chlorella vulgaris Species 0.000 description 1
- 235000007089 Chlorella vulgaris Nutrition 0.000 description 1
- 241001501885 Isochrysis Species 0.000 description 1
- 241000224474 Nannochloropsis Species 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 241001310885 Vitreochlamys Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000003050 macronutrient Effects 0.000 description 1
- 235000021073 macronutrients Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000027870 phototropism Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000013468 resource allocation Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000010019 sublethal effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q3/00—Condition responsive control processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/20—Mixing gases with liquids
- B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
- B01F23/231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/20—Mixing gases with liquids
- B01F23/29—Mixing systems, i.e. flow charts or diagrams
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
Some aspects of the invention can be related to a kind of algae culture bioreactor spraying system, which may include the lighting system for algal grown.Bioreactor may include the first injector and the second injector.The system can also include at least one light source, which illuminates the inside of bioreactor；With at least one controller, which controls the illumination photons flux density of at least one light source.Bioreactor can it is illuminated with provide be more than daily in bioreactor 90% maximum algal grown amount, and controller may be configured to control the illumination wavelengths of at least one light source.
Description
Invention field
Present invention relates in general to algal growns.More particularly, the present invention relate to enhance the system of algal grown and
Method.
Background of invention
In recent years, become using algae culture of the bioreactor (for example, utilizing bubble tower) under artificial condition
It is more and more common, such as to produce biomass.For the growth of optimum condition and acceleration, algae (or microalgae) is supplied with richness
Containing CO2Air bubble and illumination (artificial light or come from sunlight).About 50% algal biomass is carbon, by photosynthetic solid
Determine CO2It obtains, wherein carbon dioxide is needed with liquid phase dissolved in culture.In phototropism algae culturing system, for giving birth to
Long main input (or magnanimity nutrients (macro-nutrients)) is light, CO2, nutrients (nitrogen, phosphorus etc.), and
Water with turbulent closure scheme, to give these resource allocations to single algae culture cell.
Furthermore, it is necessary to which good fluid is mixed for realizing high algae concentration in the bioreactor.Good mixing can
To control the exposure of cell by reducing the degree mutually covered and minimizing Xanthophyll cycle.Effective mixing can be such that cell moves
It is dynamic to be inputted close to illuminated surface with obtaining photon, and then far from it, to give before cell is again exposed to light
The cell for giving photon saturation absorbs this luminous energy for photosynthetic chance.Since the cell concentration of superelevation is needed using strong
Light source, therefore insufficient mixing may cause and be excessively exposed to strong light, and cell may also be caused to damage due to Xanthophyll cycle
Wound.
Gas injection (is mainly rich in CO2Air or nitrogen) be commonly used in light-bioreactor (PBR), to produce
Raw required mixing.The ascending motion generation and the tangent mixing in flow direction of bubble.Effective mixing usually requires continuous
High flow rate and air pocket.However, using jet-stream wind for mixing and its composition being made to be enriched with CO2With intrinsic inefficient
Rate, because of CO2It is introduced in air pocket (needed for mixing) with diluted concentration, therefore leads to the CO of about 10% difference2Biology
Utilization rate (wherein about 90% CO2It is discharged from bioreactor).
Microalgae can be grown to illumination in the system such as Flat photobioreactor of many types.For algal grown
Light source can be any kind of visible light in the range of about 400nm-700nm wavelength.Light emitting diode (LED) has
Specific wavelength, such as the ability of the light in visible light (for example, blue and/or red) wave-length coverage are provided.
However, it is some input become it is limited (for example, due to algae from cover caused by restricted light), and cause to
Determine the maximal density of the determination of algae in system.If every other input is supplied with non-limiting availability, with algae
The density of culture increases, and cell covers the cell being blocked in optical path.Finally, light cannot penetrate into culture far enough
In, to allow more growths, and system reaches its maximum (light is limited) concentration.
Summary of the invention
Some aspects of the invention can be related to algae culture container spraying system.The system may include: at least one
Sensor, at least one parameter at least one sensor measurement container；At least one first injector, this at least one
First fluid is assigned in container by the first injector with the first operation flow velocity；And at least one second injector, this is at least
Second fluid is assigned in container based on the parameter that at least one is measured with the second operation flow velocity by one the second injector.One
In a little embodiments, which can also include at least one light source, the inside of at least one light source Illumination container；At least
One controller, the first operation flow velocity of at least one controller control and the second operation flow velocity.In some embodiments, it controls
Device processed may be configured to control the illumination wavelengths of at least one light source, and the first operation flow velocity may be adapted to allow cultivating
Turbulent closure scheme algae in container, and the second operation flow velocity may be adapted to allow the same of the material in the liquid in culture vessel
Change.
In some embodiments, controller may be configured to control at least one light source with 650 nanometers of wavelength
It is illuminated.
Some aspects of the invention can be related to the bioreactor lighting system for algal grown.The system can wrap
At least one light source is included, which illuminates the inside of bioreactor；With at least one controller, this at least one
Controller controls the illumination photons flux density of at least one light source.In some embodiments, bioreactor can be shone
It is bright with provide be more than daily in bioreactor 90% maximum algal grown amount, and controller may be configured to control
The illumination wavelengths of at least one light source.
In some embodiments, the illumination photons flux density of at least one light source can be 1200 micromoles/m2/s。
In some embodiments, at least one light source can be light emitting diode.In some embodiments, at least one of algae
Dividing is Isochrysis galbana.In some embodiments, for every square metre, which may include at least four light sources.One
In a little embodiments, controller may be configured to control at least one light source to be illuminated with 650 nanometers of wavelength.
Brief description
It is considered as subject of the present invention and is specifically referred and is distinctly claimed in the latter end of specification.
However, when being read together with attached drawing, by reference to following detailed description, it is well understood that the present invention is about tissue and behaviour
The method of work and its objects, features and advantages, in which:
Fig. 1 illustrates schematically the frame of the algae culture container spraying system of some embodiments according to the present invention
Figure；
Fig. 2A illustrate schematically some embodiments according to the present invention at least one lighting unit
The block diagram of algae culture container spraying system；
Fig. 2 B illustrate schematically some embodiments according to the present invention have at least one lighting unit 201
With the block diagram of the algae culture container spraying system 200 of single injector；With
Fig. 3 shows the flow chart of the method for the injection algae culture container of some embodiments according to the present invention.
It will be understood that element shown in the accompanying drawings is not drawn necessarily to scale in order to simple and clearly demonstrate.For example, being
For the sake of clear, the size of some elements can be amplified relative to other elements.In addition, in the case where being deemed appropriate, ginseng
The corresponding or similar element of instruction can be repeated among the figures to by examining number.
The detailed description of the embodiment of invention
In the following detailed description, numerous specific details are set forth in order to provide thorough understanding of the present invention.However, this
Field the skilled person will understand that, the present invention can practice without these specific details.In other cases, do not have
Well known method, program and component are described in detail, so as not to keep the present invention fuzzy.
Referring now to Figure 1, its algae culture container for illustrating schematically some embodiments according to the present invention
The block diagram of spraying system 100.It should be noted that the direction of arrow can indicate the direction of information flow in Fig. 1.
In some embodiments, spraying system 100 may include at least one first spray with more than one nozzle
Emitter 101, first injector 101 operate flow velocity for the first scheduled fluid (for example, air and/or nitrogen bubble) with first
It is assigned in the algae culture container 10 (for example, bioreactor) of water filling, to allow to mix wherein.Spraying system
100 can also include at least one second injector 102 with more than one nozzle, and second injector 102 is with the second behaviour
Make flow velocity by the second scheduled fluid (e.g., including there is CO for mass transfer2And/or the gas bubbles of the phosphorus of dissolution)
It is assigned in container 10.
In some embodiments, spraying system 100 may include at least one controller 103, with the first operation of control
Flow velocity and the second operation flow velocity.According to some embodiments, at least one of the first injector 101 and the second injector 102 are sprayed
Mouth can be distributed a fluid in culture vessel 10 based on the request from least one controller 103, as follows further to retouch
It states.In some embodiments, the first operation flow velocity can be based on the second operation flow velocity.In some embodiments, first
It is scheduled for operating at least one of flow velocity and the second operation flow velocity.
In some embodiments, the first operation flow velocity may be adapted to the turbulent closure scheme for allowing algae in culture vessel 10.
In some embodiments, the second operation flow velocity may be adapted to the mass transfer for allowing the material in the liquid in culture vessel 10
And/or assimilation.
In some embodiments, the second scheduled fluid may include having more than 30%CO2The gas bubbles of concentration.
According to some embodiments, the source of at least one first scheduled fluid and the second scheduled fluid can be in spraying system
100 outside, such as geothermal power station can provide the carbon of dissolution and/or the source of sulphur for the second scheduled fluid.
In some embodiments, the first operation flow velocity of at least one nozzle of the first injector 101 is (for example, 100 millis
M/min) can be different from the second injector 102 at least one nozzle second operation flow velocity (for example, 5 mm/mins).
In some embodiments, at least one nozzle of the first injector 101 can have straight greater than~1 millimeter
Diameter.In some embodiments, at least one nozzle of the second injector 102 can have the diameter less than~1 millimeter.One
In a little embodiments, the nozzle of the nozzle of the first injector 101 and the second injector 102 can distribute identical fluid (example
Such as air), wherein the nozzle of each injector has different diameters.
In some embodiments, spraying system 100 can also include physical barriers 104, to be separately cultured in container 10
The first fluid distributed by the first injector 101 and the second fluid distributed by the second injector 102.In some embodiments
In, at least one nozzle of the first injector 101 and/or the second injector 102 can be embedded in physical barriers 104.In
In some embodiments, physical barriers 104 may be adapted to allow in predetermined (for example, upper and lower part) position of culture vessel 10
The other side (there is second fluid distribution) is flowed to from the side (distributing with first fluid) of barrier, to generate in container 10
Controlled flowing.
In some embodiments, spraying system 100 can also include at least one sensor 105 (for example, temperature sensing
Device), at least one sensor 105 be coupled to controller 103 and be configured to detect in culture vessel 10 at least one
A feature.For example, at least one sensor 105 can detecte, the pH in culture vessel 10 is horizontal, in temperature and pressure condition
At least one.In some embodiments, at least one sensor 105 can also detect the parameter outside culture vessel 10, example
As measure the gaseous emission from culture vessel 10 mass flow, with by from be inserted into container (for example, by second spray
Emitter 102) amount in subtract the amount of discharge to determine the amount for the substance being absorbed into alga cells.
In some embodiments, spraying system 100 can also include at least one database 106 (or memory list
Member), it is configured to store the algorithm for operating controller 103, such as the behaviour for each nozzle and/or each injector
Make the database of rate.In some embodiments, spraying system 100 can also include power supply 107, which is coupled to
It to controller 103 and is configured to provide electric power to spraying system 100, thus power supply 107 is suitable for at least one the first injection
Device 101 and at least one second injector 102 are powered to operate at a different rate.
In some embodiments, the data collected by least one sensor 105 can pass through controller (or processing
Device) 103 analyze, with detect attribute whether be more than pH in scheduled threshold value, such as container 10 horizontal and/or temperature and/or
CO2The threshold value of concentration.It is more than at least one threshold in the condition (for example, as detected by sensor 105) in culture vessel 10
In the case where value, then controller 103 can operate at different flow rates the first injector 101 at least one nozzle and/or
At least one nozzle of second injector 102.For example, the CO in detection container 102Concentration (or the pH water that detection is low that is more than 40%
It is flat) it can cause at least one nozzle of the second injector 102 that the flow velocity of second injector 102 is reduced to~2 millis m/min
Clock.In some embodiments, at least one nozzle of the second injector 102 only can receive attribute from sensor 105
More than scheduled threshold value signal when operate, and not with constant rate operation.
In some embodiments, at least one nozzle of the first injector 101 can be received only from sensor 105
Attribute be more than scheduled threshold value signal when operate, such as with the density of algae colony increase and increase mixed traffic.Root
According to some embodiments, at least one nozzle of at least one nozzle of the first injector 101 and/or the second injector 102 can
It is continuous thus to operate not with constant rate operation.According to some embodiments, the first injector 101 at least one
At least one nozzle of nozzle and/or the second injector 102 can be operated with rate non-constant, and thus operation is nor continuous
's.
In some embodiments, culture vessel 10 can have bubbling column structure, the first injector of wherein at least one
101 are positioned on the same surface of bubble tower container at least one second injector 102.In some embodiments, it trains
Supporting container 10 can have gas lift configuration, and the second injector of wherein at least one 102 is positioned in down-comer (down-comer)
Bottom part, may be located remotely from sensor 105, so that the residence time of the bubble from least one the second injector 102
It can increase.
In some embodiments, spraying system 100 can permit in container 10 at least 20% organic carbon, compared to work
For CO2What the carbon that bubble provides calculated.In some embodiments, at least part algae in container 10 is chlorella
(Chlorella Vulgaris).In some embodiments, at least part algae in container 10 is micro- Sphaerellopsis
(Nannochloropsis).In some embodiments, at least part algae in container 10 is Isochrysis galbana
(Isochrysis galban)。
Referring now to Fig. 2A, illustrate schematically some embodiments according to the present invention has at least one
The block diagram of the algae culture container spraying system 200 of lighting unit 201.It should be noted that the direction of arrow can indicate in Fig. 2A
The direction of information flow.
In some embodiments, algae culture container spraying system 200 may include that at least one is coupled to control
The lighting unit 201 of device 103, to illuminate culture vessel 10.In some embodiments, at least one lighting unit 201 and control
Device 103 (or another controller) processed can be included in the bioreactor lighting system 208 for algal grown.One
In a little embodiments, the distance between culture vessel 10 and at least one lighting unit 201 can be modified to control by training
Support the received illumination of container 10.For example, make at least one lighting unit 201 closer to culture vessel 10, it is therein to increase
The illumination of algae.In some embodiments, the distance between culture vessel 10 and at least one lighting unit 201 can be by examples
It such as include the control of controller 103 in lighting system 208.According to some embodiments, in addition to or instead of change lighting unit
201 distances away from culture vessel 10 can control the illumination intensity light source 202 in lighting unit 201.
In some embodiments, at least one lighting unit 201 may include at least one light source 202 (for example,
LED), it is individually controlled each light source 202 by controller 103.In some embodiments, at least one light source
202 can be controlled to the intensity light illumination different from another light source 202.According to some embodiments, all light sources 202 can be with
It is controlled to change illumination intensity manually or according to the condition sensed in preset timing and/or culture vessel 10.
In some embodiments, the culture vessel 10 with physical barriers 104 may include being embedded in physical barriers
At least one light source 202 (as shown in Figure 1) in 104, allows container 10 from inside, i.e., from being embedded in physics screen
At least one light source 202 in barrier 104 is illuminated.According to some embodiments, culture vessel 10 may include more than one object
Barrier 104 is managed, each physical barriers include at least one light source 202, allow to creation module system, wherein algae is in phase
It is grown between adjacent physical barriers 104, wherein at least one controller 103, which can control, to be embedded in physical barriers 104
The illumination of all light sources 202.
It such as can be it is evident that being transported to the amount of the light of culture vessel 10 can be determined for those of ordinary skill in the art
Justice is the average value for being transported to the luminous flux on surface of culture vessel 10.Therefore, for ultra high density culture (for example, density
Higher than~5 grams per liters) spraying system 200, at least one lighting unit 201 can have the light point of at least one light source 202
Match, in order to provide the average light for the average flux for being substantially equal to low density cell culture object (for example, density is lower than~5 grams per liters)
Flux, realizes similar light infiltration, at the same at least one lighting unit 201 each light source 202 can have it is higher strong
Degree.In some embodiments, the luminous intensity in culture vessel 10 can be measured at least one sensor 105.
For example, for ultra high density culture, light-path can be short (for example,~1 millimeter -5 millimeters of illumination region
And~20 millimeters -30 millimeters of dark area), allow the alga cells of proximity illumination unit 201 by Xanthophyll cycle (to algae
The sublethal effect of class) and/or by photobleaching (to the lethal effect of algae), therefore lighting unit 201 can initially and container
The 10 some growths maintained a certain distance to allow algae, and then closer to (for example, once a day), so as to further
Increase algal grown.In some embodiments, due to short light-path, ultra high density culture may need to mix, so as to
It is allowed for the illumination cycle of algae (between illumination region and dark area).In some embodiments, ultra high density is trained
Feeding object can use various wavelength illuminations, because, due to short light-path, wavelength may not almost have growth under such density
Have an impact.It should be noted that according to the common practice, algae is illuminated with specific wavelength (for example, with blue light), for normally raw
It is long, because algae should differently respond light, however the illumination with any wavelength is had been shown by the experiment that applicant carries out
It can be used for ultra high density culture.
According to some embodiments, light, which penetrates into culture vessel 10, can correspond to luminous intensity, optical wavelength, specific algae
At least one of class strain and/or algal cultures density.It should be noted that light, which penetrates into, can determine culture in culture vessel 10
Distribution (ration) between illumination region and dark area in container 10, and therefore can influence by lighting unit 201
The luminous intensity of offer, by the gas flow rate of the first injector 101, pass through gas flow rate of the second injector 102 etc..
In some embodiments, culture vessel 10 can be illuminated by least one lighting unit 201, cultivated with providing
It is daily more than the amount of 90% maximum algal grown in container 10.
In some embodiments, at least one lighting unit 201 may include that the low distribution of high-intensity light source 202 is matched
It sets.It is such to configure the algae that can permit enhancing compared with the common practice configuration evenly distributed with low intensity light source
Class growth.In some embodiments, the illumination photons flux density of at least one light source 202 is 1200 micromoles/rice2/ the second.
In some embodiments, for every square metre, at least one lighting unit 201 may include at least four light sources 202.Example
It such as, may include 24 LED light sources 202 with about 6 square metres of surface area, the lighting unit 201 of optical path of about 4cm, each
LED light source 202 has 1200 micromoles/rice2The luminous flux of/second.In some embodiments, at least part in container 10
Algae is Isochrysis galbana.
In some embodiments, controller 103 may be configured to control the illumination wavelengths of at least one light source 202,
Such as the dedicated lighting module of the wavelength using the illumination for being suitably modified to transmitting.It in some embodiments, can be in container
27 DEG C of steady temperature is maintained in 10.
In some embodiments, controller 103 may be configured to control at least one light source 202 with 650 nanometers
Wavelength illuminated.It should be noted that according to the common practice, algae is illuminated with specific wavelength (for example, with blue light), it is used for
Optimum growh, however had been shown by the experiment that applicant carries out and be can be used for the illumination of other wavelength (for example, with feux rouges)
The growth of enhancing.
Referring now to Fig. 2 B, illustrate schematically some embodiments according to the present invention has at least one
The block diagram of the algae culture container spraying system 210 of lighting unit 201 and single third injector 211.It should be noted that in Fig. 2 B
The direction of arrow can indicate the direction of information flow.
In some embodiments, spraying system 210 may include that at least one has at least one third injector 211
The lighting unit 201 of (at least one nozzle), the third injector 211 are configured to scheduled fluid being assigned to culture
In container 10.In some embodiments, at least one third injector 211 may include distributing the first scheduled fluid extremely
At least one nozzle (for example, there is different diameters) of the second scheduled fluid of a few nozzle and distribution.In some implementations
In scheme, at least one third injector 211 may be adapted to allow the turbulent closure scheme algae in culture vessel 10, and be suitable for permitting
Perhaps CO in the liquid in container 102Assimilation.
Referring now to Figure 3, it illustrates the sides of the injection algae culture container 10 of some embodiments according to the present invention
The flow chart of method.In some embodiments, this method may include 301 at least one first injector 101 of control the
First fluid is assigned in container 10 by one operation flow velocity.In some embodiments, this method can also include control 302 the
Second fluid to be assigned in container 10 by two injectors 102 in the second operation flow velocity.In some embodiments, at least one
First operation flow velocity of the first injector 101 can be different from the second operation flow velocity of at least one the second injector 102.One
In a little embodiments, this method can also include at least one parameter in 303 containers 10 of measurement, and according at least one survey
The parameter of amount changes to change the operation flow velocity of at least one of 304 at least one second injector 102.
In some embodiments, the first operation flow velocity may be adapted to allow the turbulent closure scheme algae in culture vessel, and
And second operation flow velocity may be adapted to allow culture vessel in liquid in material assimilation.
Unless explicitly stated otherwise, otherwise method described herein embodiment is not limited to specific chronological order or the time is suitable
Sequence.It in addition, during the operation order of method, can skip some in described method element, or it can be repeated
.
Have been presented for various embodiments.Each of these embodiments can of course include from being presented
The feature of other embodiments, and the embodiment being not specifically described may include various features described herein.
Claims (8)
1. a kind of algae culture container spraying system, comprising:
At least one sensor, at least one parameter at least one described sensor measurement container；
First fluid is assigned to institute with the first operation flow velocity by least one first injector, at least one described first injector
It states in container；
At least one second injector, at least one described second injector is based on the parameter that at least one is measured with the second operation
Second fluid is assigned in the container by flow velocity；
At least one light source, at least one described light source illuminate the inside of the container；With
At least one controller, at least one controller control first operation flow velocity and the second operation flow velocity,
Wherein the controller is configured to control the illumination wavelengths of at least one light source, wherein the first operation flow velocity
It is adapted to allow for the turbulent closure scheme algae in the culture vessel, and wherein the second operation flow velocity is adapted to allow for the culture
The assimilation of the material in liquid in container.
2. system as claimed in claim 4, wherein the controller is configured to control at least one light source with 650 nanometers
Wavelength illuminated.
3. a kind of bioreactor lighting system for algal grown, the system comprises:
At least one light source, the inside of at least one light source illumination bioreactor；With
At least one controller, at least one described controller control the illumination photons flux density of at least one light source,
Wherein the bioreactor is illuminated to provide the maximum algal grown in the bioreactor being daily more than 90%
Amount, and wherein the controller is configured to control the illumination wavelengths of at least one light source.
4. system as claimed in claim 3, the illumination photons flux density of wherein at least one light source is 1200 micro- to rub
That/m2/s。
5. system as claimed in claim 3, wherein at least one light source is light emitting diode.
6. system as claimed in claim 3, wherein at least part of the algae is Isochrysis galbana.
7. system as claimed in claim 3, every square metre includes at least four light sources.
8. system as claimed in claim 3, wherein the controller is configured to control at least one light source with 650 nanometers
Wavelength illuminated.
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| US201762449045P | 2017-01-22 | 2017-01-22 | |
| US62/449,045 | 2017-01-22 | ||
| PCT/IL2018/050066 WO2018134819A1 (en) | 2017-01-22 | 2018-01-18 | System and method for growing algae |
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| CN110494547A true CN110494547A (en) | 2019-11-22 |
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| US11883377B1 (en) | 2022-09-22 | 2024-01-30 | Vaxa Technologies Ltd | Algal botanical extracts rich in eicosapentaenoic acid as tri/di-glyceride conjugate |
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| JP2020513841A (en) | 2020-05-21 |
| JP3239404U (en) | 2022-10-07 |
| WO2018134819A1 (en) | 2018-07-26 |
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