CN110484364A - A method of extracting arachidonic acid oil from arachidonic acid emulsion - Google Patents
A method of extracting arachidonic acid oil from arachidonic acid emulsion Download PDFInfo
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- CN110484364A CN110484364A CN201910774879.8A CN201910774879A CN110484364A CN 110484364 A CN110484364 A CN 110484364A CN 201910774879 A CN201910774879 A CN 201910774879A CN 110484364 A CN110484364 A CN 110484364A
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- arachidonic acid
- emulsion
- temperature
- acid oil
- oil
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B7/00—Separation of mixtures of fats or fatty oils into their constituents, e.g. saturated oils from unsaturated oils
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Edible Oils And Fats (AREA)
- Fats And Perfumes (AREA)
Abstract
The method that the present invention provides a kind of to extract arachidonic acid oil from arachidonic acid emulsion, comprising: first will be enriched in arachidonic emulsion rapidly extremely -5~5 DEG C of temperature control, and keep the temperature 2h；The emulsion after temperature control is warming up to 65~95 DEG C again, 40-60min is kept the temperature, isolates arachidonic acid oil using three-phase centrifuge.The present invention is extracted using physical method, and recovery rate is high, and nutrition deterioration is low, and obtained arachidonic acid oil quality is good.
Description
Technical field
The present invention relates to arachidonic acid preparation technical fields, mention from arachidonic acid emulsion more particularly, to one kind
The method for taking arachidonic acid oil.
Background technique
Arachidonic acid (ARA), is all-cis formula-Arachidonic Acid, belongs to unsaturated fatty acid, wherein
Containing there are four carbon-to-carbon double bond, a carbon-oxygen double bond is higher unsaturated fatty acid.It is distributed widely in the animal kingdom, exists on a small quantity
In the glyceride of some kind, it can also find in glycerophosphatide class, can be synthesized by linoleic acid in human body.ARA is as breast milk
Component part, in neuro-physiology and neurology, the growth to baby be it is necessary, due to arachidonic in infants
The combined coefficient of acid is very low, and synthetic quantity is insufficient for the demand of body development, so must additionally supplement from food.
Currently, all being divided substantially using chemical extraction from rich in extraction arachidonic acid oil in arachidonic emulsion
From method, be easy Oxidative demage is caused to arachidonic acid, recovery rate is not high, and recovery rate is unstable, it is difficult to guarantee peanut
The quality of tetraene acid lipid.
Summary of the invention
The method that the object of the present invention is to provide a kind of to extract arachidonic acid oil from arachidonic acid emulsion, should
The method that method uses physics to extract, recovery rate is high, and the quality of obtained arachidonic acid oil is stablized, to solve the prior art
Present in the above problem.
In order to achieve the above object, the invention provides the following technical scheme:
A method of extracting arachidonic acid oil from arachidonic acid emulsion, comprising: first will be enriched in peanut four
Rapidly temperature control and keeps the temperature 2h to -5~5 DEG C to the emulsion of olefin(e) acid；The emulsion after temperature control is warming up to 65~95 DEG C again, heat preservation
40-60min isolates arachidonic acid oil using three-phase centrifuge.
Further, it specifically includes, the emulsion after temperature control is first slowly cycled through into one section of glass pipe, glass pipe
Under the conditions of irradiated heat, emulsion is warming up to 35~65 DEG C by irradiated heat mode, 20-30min is kept the temperature, reuses
Steam-heated mode continues emulsion to be heated to 65~95 DEG C, keeps the temperature 20-30min.
It further, further include that quality point is added in through the isolated arachidonic acid oil of three-phase centrifuge
Number is 10% citric acid solution, stirs 30min under the conditions of 55 DEG C~65 DEG C, then separated by two-phase centrifuge, finally
After being cleaned 1~3 time using pure water, vacuum dehydration obtains arachidonic acid crude oil of the water content less than 0.1%.
Further, the irradiated heat mode includes microwave heating or infrared heating, but not limited to this.
Further, it first will be enriched in arachidonic emulsion rapidly extremely -5~1 DEG C of temperature control, and keep the temperature 2h；By temperature control
Emulsion afterwards first slowly cycles through one section of glass pipe, and glass pipe is under the conditions of irradiated heat, passes through irradiated heat
Emulsion is warming up to 45~60 DEG C by mode, is kept the temperature 20-30min, is reused steam-heated mode and continue to heat by emulsion
To 85~95 DEG C, 20-30min is kept the temperature, isolates arachidonic acid oil using three-phase centrifuge.
Further, the concentration of arachidonic acid oil is 1.5g/L~60g/L in the arachidonic acid emulsion.
The method that arachidonic acid oil is extracted in slave arachidonic acid emulsion of the invention, is carried out using physical method
It extracts, recovery rate is high, and nutrition deterioration is low, and obtained arachidonic acid oil quality is good, wherein glycerol in arachidonic acid oil
The 90%~97% of the total oil of three rouge Zhan, other components are monoglyceride, diglyceride and free fatty acid.In addition, obtained peanut four
Water content in olefin(e) acid grease is not higher than 10%, and other impurities content is not higher than 5%.
Specific embodiment
Technical solution of the present invention is clearly and completely described below, it is clear that described embodiment is only this
Invention a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art exist
Every other embodiment obtained under the premise of creative work is not made, shall fall within the protection scope of the present invention.
Embodiment 1
In a kind of preferable embodiment of the present invention, the side of arachidonic acid oil is extracted from arachidonic acid emulsion
In method, solid concentration is 1%~20% in arachidonic acid emulsion, and triglyceride levels are 5g/L~100g/L, emulsification
The concentration of arachidonic acid oil is 1.5g/L~60g/L in liquid.
Wherein, the composition of emulsion are as follows: water accounts for 80%~99%, and polysaccharose substance accounts for 0.3%~8%, polypeptide and amino
Acid accounts for 0.1%~3%, and triglyceride accounts for 0.5%~10%.
Extracting method specifically includes: first will be enriched in arachidonic emulsion rapidly extremely -1 DEG C of temperature control, and keeps the temperature 2h；It will
Emulsion after temperature control first slowly cycles through one section of glass pipe, and glass pipe is under the conditions of irradiated heat, passes through irradiation
Emulsion is warming up to 50 DEG C by heating method, is kept the temperature 30min, is reused steam-heated mode and continue emulsion to be heated to 90
DEG C, 30min is kept the temperature, isolates arachidonic acid oil using three-phase centrifuge.The irradiated heat mode includes that microwave adds
Heat or infrared heating.
Embodiment 2
It further include being separated by three-phase centrifuge after obtaining arachidonic acid oil in embodiment 1 in the present embodiment
The arachidonic acid oil arrived, it is 10% citric acid solution that mass fraction is added thereto, stirs 30min under the conditions of 65 DEG C,
It is separated again by two-phase centrifuge, after finally being cleaned 1~3 time using pure water, vacuum dehydration obtains water content and is less than
0.1% arachidonic acid crude oil.
Embodiment 3
In present embodiment, solid concentration is 1%~20% in arachidonic acid emulsion, and triglyceride levels are
5g/L~100g/L, the concentration of arachidonic acid oil is 1.5g/L~60g/L in emulsion.
Wherein, the composition of emulsion are as follows: water accounts for 80%~99%, and polysaccharose substance accounts for 0.3%~8%, polypeptide and amino
Acid accounts for 0.1%~3%, and triglyceride accounts for 0.5%~10%.
Extracting method specifically includes: first will be enriched in arachidonic emulsion rapidly extremely -3 DEG C of temperature control, and keeps the temperature 2h；It will
Emulsion after temperature control first slowly cycles through one section of glass pipe, and glass pipe is under the conditions of irradiated heat, passes through irradiation
Emulsion is warming up to 65 DEG C by heating method, is kept the temperature 20min, is reused steam-heated mode and continue emulsion to be heated to 85
DEG C, 30min is kept the temperature, isolates arachidonic acid oil using three-phase centrifuge.The irradiated heat mode includes that microwave adds
Heat or infrared heating.
Embodiment 4
In present embodiment, solid concentration is 1%~20% in arachidonic acid emulsion, and triglyceride levels are
5g/L~100g/L, the concentration of arachidonic acid oil is 1.5g/L~60g/L in emulsion.
Wherein, the composition of emulsion are as follows: water accounts for 80%~99%, and polysaccharose substance accounts for 0.3%~8%, polypeptide and amino
Acid accounts for 0.1%~3%, and triglyceride accounts for 0.5%~10%.
Extracting method specifically includes: first will be enriched in arachidonic emulsion, rapidly temperature control and keeps the temperature 2h to 1 DEG C；It will
Emulsion after temperature control first slowly cycles through one section of glass pipe, and glass pipe is under the conditions of irradiated heat, passes through irradiation
Emulsion is warming up to 60 DEG C by heating method, is kept the temperature 20min, is reused steam-heated mode and continue emulsion to be heated to 95
DEG C, 20min is kept the temperature, isolates arachidonic acid oil using three-phase centrifuge.The irradiated heat mode includes that microwave adds
Heat or infrared heating.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (6)
1. a kind of method for extracting arachidonic acid oil from arachidonic acid emulsion, characterized by comprising: first will be rich
Containing arachidonic emulsion, rapidly temperature control and keeps the temperature 2h to -5~5 DEG C；The emulsion after temperature control is warming up to 65~95 again
DEG C, 40-60min is kept the temperature, isolates arachidonic acid oil using three-phase centrifuge.
2. the method according to claim 1 for extracting arachidonic acid oil from arachidonic acid emulsion, feature
It is: specifically includes, the emulsion after temperature control is first slowly cycled through into one section of glass pipe, glass pipe is in irradiated heat
Under the conditions of, emulsion is warming up to 35~65 DEG C by irradiated heat mode, 20-30min is kept the temperature, reuses steam-heated side
Formula continues emulsion to be heated to 65~95 DEG C, keeps the temperature 20-30min.
3. the method according to claim 1 or 2 for extracting arachidonic acid oil from arachidonic acid emulsion, special
Sign is: further including that it is 10% lemon that mass fraction, which is added, in through the isolated arachidonic acid oil of three-phase centrifuge
Lemon acid solution stirs 30min under the conditions of 55 DEG C~65 DEG C, then is separated by two-phase centrifuge, finally clear using pure water
After washing 1~3 time, vacuum dehydration obtains arachidonic acid crude oil of the water content less than 0.1%.
4. the method according to claim 2 for extracting arachidonic acid oil from arachidonic acid emulsion, feature
Be: the irradiated heat mode includes microwave heating or infrared heating.
5. the method according to claim 2 for extracting arachidonic acid oil from arachidonic acid emulsion, feature
It is: first will be enriched in arachidonic emulsion rapidly extremely -5~1 DEG C of temperature control, and keep the temperature 2h；Emulsion after temperature control is first
One section of glass pipe slowly is cycled through, glass pipe is under the conditions of irradiated heat, by irradiated heat mode by emulsion
45~60 DEG C are warming up to, 20-30min is kept the temperature, steam-heated mode is reused and continues emulsion to be heated to 85~95 DEG C, protect
Warm 20-30min isolates arachidonic acid oil using three-phase centrifuge.
6. the method according to claim 1 for extracting arachidonic acid oil from arachidonic acid emulsion, feature
Be: the concentration of arachidonic acid oil is 1.5g/L~60g/L in the arachidonic acid emulsion.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910774879.8A CN110484364A (en) | 2019-08-21 | 2019-08-21 | A method of extracting arachidonic acid oil from arachidonic acid emulsion |
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| CN201910774879.8A CN110484364A (en) | 2019-08-21 | 2019-08-21 | A method of extracting arachidonic acid oil from arachidonic acid emulsion |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103922921A (en) * | 2014-04-17 | 2014-07-16 | 青岛琅琊台集团股份有限公司 | Method of extracting ARA (arachidonic acid) from fermentation liquor |
| CN104962589A (en) * | 2015-08-05 | 2015-10-07 | 嘉必优生物工程（武汉）有限公司 | Microbial oil rich in phospholipid type polyunsaturated fatty acid and preparation method thereof |
| CN106755151A (en) * | 2017-02-23 | 2017-05-31 | 内蒙古金达威药业有限公司 | A kind of method that utilization microbial fermentation produces ARA |
| CN106977394A (en) * | 2016-12-01 | 2017-07-25 | 烟台固特丽生物科技股份有限公司 | A kind of technique of the low colourity arachidonic acid oil of rapid extraction |
| CN109181843A (en) * | 2018-08-20 | 2019-01-11 | 梁云 | The device and method and microbial oil of on-line heating separate microorganism grease |
-
2019
- 2019-08-21 CN CN201910774879.8A patent/CN110484364A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103922921A (en) * | 2014-04-17 | 2014-07-16 | 青岛琅琊台集团股份有限公司 | Method of extracting ARA (arachidonic acid) from fermentation liquor |
| CN104962589A (en) * | 2015-08-05 | 2015-10-07 | 嘉必优生物工程（武汉）有限公司 | Microbial oil rich in phospholipid type polyunsaturated fatty acid and preparation method thereof |
| CN106977394A (en) * | 2016-12-01 | 2017-07-25 | 烟台固特丽生物科技股份有限公司 | A kind of technique of the low colourity arachidonic acid oil of rapid extraction |
| CN106755151A (en) * | 2017-02-23 | 2017-05-31 | 内蒙古金达威药业有限公司 | A kind of method that utilization microbial fermentation produces ARA |
| CN109181843A (en) * | 2018-08-20 | 2019-01-11 | 梁云 | The device and method and microbial oil of on-line heating separate microorganism grease |
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Application publication date: 20191122 |