CN110477196A - The application of glycerol and rumen bypass becholine in preparation prevention and/or treatment sheep eclampsia feed addictive or drug - Google Patents

Abstract

The invention discloses the application of glycerin and rumen-passed choline chloride in the preparation of feed additives or medicines for preventing and/or treating Hu sheep pregnancy toxemia; the invention proposes a new function of glycerin and rumen-passed choline chloride , can be used as an additive or drug in the diet of Hu sheep in the late pregnancy to prevent and treat pregnancy toxemia in Hu sheep, reduce the mortality of the disease, and improve economic benefits. In the present invention, glycerin can be degraded by rumen microorganisms in the rumen to produce volatile fatty acids to provide energy for the body. In addition, glycerin can also be absorbed into the blood and serve as a substrate for gluconeogenesis to relieve negative energy balance. Choline chloride can be combined with triglyceride in the liver and transported out of the liver in the form of very low-density lipoprotein, thereby alleviating fatty liver and maintaining normal liver function. In the present invention, glycerin and rumen-passed choline chloride are used as additives to synergize synergistically and have better effects.

Description

Glycerin and rumen-passed choline chloride in the preparation of prevention and/or treatment of Hu sheep pregnancy toxemia application in feed additives or medicines

technical field

The invention belongs to the field of animal feed additives and medicines, and in particular relates to the application of glycerin and rumen-passed choline chloride in the preparation of feed additives or medicines for preventing and/or treating Hu sheep pregnancy toxemia.

Background technique

Late pregnancy is the high incidence stage of pregnancy toxemia in Hu sheep. Hu sheep are favored by producers because of their multiple lambs, but multiple lambs lead to increased fetal energy requirements. Studies have shown that 80% of fetal growth and development occur in the late pregnancy. At this time, on the one hand, due to the sharp increase in energy demand for fetal growth and development; on the other hand, the rapid growth and development of multiple lambs caused the rapid expansion of the uterus, which in turn mechanically squeezed the rumen space, resulting in a decrease in feed intake for ewes. Energy intake is reduced. The two aspects together aggravate the negative energy balance of ewes. Negative energy balance can lead to weak lambs and dead lambs, and seriously can lead to the death of ewes, causing significant economic losses to Hu sheep production. However, there are currently no effective measures for the prevention and treatment of pregnancy toxemia in Hu sheep. Studies have shown that glycerol can be degraded by rumen microorganisms in the rumen to produce volatile fatty acids to provide energy for the body. In addition, glycerol can also be absorbed into the blood and serve as a substrate for gluconeogenesis to relieve negative energy balance. In addition, under the condition of negative energy balance, Hu sheep responsively mobilized body fat for β-oxidation, resulting in elevated blood ketones and fatty liver. Studies have shown that choline chloride can be combined with triglycerides in the liver and transported out of the liver in the form of very low-density lipoprotein. Therefore, it is necessary to explore the application of glycerol and rumen-passed choline chloride in the prevention of pregnancy toxemia in Hu sheep.

The molecular formula of glycerin is: C ₃ H ₈ O ₃ , and the structural formula is as follows:

The molecular formula of choline chloride is: C ₅ H ₁₄ ClNO, and the structural formula is as follows:

But at present, glycerin or rumen-passed choline chloride are mostly used for postpartum ketosis in dairy cows, and there is no report on the application in the prevention of pregnancy toxemia in Hu sheep.

purpose of invention

Summary of the invention: Aiming at the problems existing in the prior art, the present invention provides the application of glycerin and rumen-passed choline chloride in the preparation of feed additives or medicines for the prevention and/or treatment of Hu sheep pregnancy toxemia. The invention proposes a new function of glycerin and rumen-passed choline chloride, which can be used as an additive or medicine in the diet of Hu sheep in the late pregnancy to prevent and treat pregnancy toxemia of Hu sheep, reduce the mortality rate of the disease, and improve economic efficiency. benefit.

Technical solution: In order to achieve the above purpose, the application of glycerin and rumen-passed choline chloride as described in the present invention in the preparation of feed additives or medicines for preventing and/or treating pregnancy toxemia in Hu sheep.

Wherein, the additive is an additive for Hu sheep feed.

Wherein, the feed is used for late pregnancy of Hu sheep.

Further, the Hu sheep are pregnant ewes with multiple lambs.

As preferably, described feed additive or medicine dosage are that glycerin addition is 40ml/ (pregnant ewe with many lambs)/day, and rumen choline chloride addition is 10g/ only (pregnant ewes with multiple lambs)/ sky.

Further, the glycerol increases the concentration of volatile fatty acids in the rumen fluid, increases the glucose level in the blood, reduces the concentration of β-hydroxybutyric acid in the blood and passes the rumen choline chloride by transporting excess free fatty acids in the liver out of the liver (increase the level of free fatty acid and very low density lipoprotein in the blood), and be used by other organizations to increase the glucose level in the blood in the preparation of feed additives or medicines for preventing and/or treating pregnancy toxemia in Hu sheep.

The results showed that glycerol was degraded by rumen microorganisms in the rumen to increase glycogenic precursors (volatile fatty acids), improve blood glucose levels, and simultaneously reduce body fat mobilization and fatty acid oxidation (β-hydroxybutyric acid concentration), thereby achieving prevention and/or Or the purpose of treating pregnancy toxemia. However, rumen-passing choline chloride can transport excessively mobilized fatty acids in the liver out of the liver in the form of very low-density lipoproteins (increased levels of free fatty acids and very low-density lipoproteins in the blood). Deposition in the liver, relieve fatty liver, maintain normal liver function (gluconeogenesis and fatty acid oxidation, etc.); consumption and relieve the negative energy balance.

The feed additive or pharmaceutical composition for preventing and/or treating Hu-sheep pregnancy toxemia according to the present invention is characterized in that it includes glycerin and rumen-passed choline chloride as active ingredients, and the feed additive or pharmaceutical composition contains Excipients are required.

Beneficial effect: compared with the prior art, the present invention has the following advantages:

The glycerin and rumen-passed choline chloride of the present invention are used as feed additives or medicines for Hu sheep to prevent and/or treat Hu sheep pregnancy toxemia, reduce the disease mortality rate and improve economic benefits. Glycerin can increase the concentration of volatile fatty acids in rumen fluid, increase the level of glucose in blood, reduce the concentration of β-hydroxybutyric acid in blood, and prevent pregnancy toxemia. Rumen-passed choline chloride can increase blood glucose levels, and increase blood free fatty acids and very low-density lipoprotein levels, suggesting that rumen-passed choline chloride can transport excess triglycerides deposited in the liver out of the liver and protect the liver Function. The reason why the rumen-passed choline chloride increases the blood glucose level is mainly through the protection of liver function, thereby improving the ability of liver gluconeogenesis. In addition, the results of the present invention show that adding glycerin and rumen-passed choline chloride at the same time has the best performance in the application of preventing pregnancy toxemia in Hu sheep.

In the present invention, glycerin can be degraded by rumen microorganisms in the rumen to produce volatile fatty acids to provide energy for the body. In addition, glycerin can also be absorbed into the blood and serve as a substrate for gluconeogenesis to relieve negative energy balance. Choline chloride can be combined with triglyceride in the liver and transported out of the liver in the form of very low-density lipoprotein, thereby alleviating fatty liver and maintaining normal liver function.

In the present invention, glycerin and rumen-passed choline chloride are used as additives to synergize synergistically and have better effects. Compared with the glycerol group alone, the addition of glycerol and rumen-passed choline chloride significantly increased the content of propionic acid in rumen fluid, suggesting that under the same fermentation substrate (glycerol), the addition of rumen-passed choline chloride may improve the metabolism of the body. , and then improve the fermentation activity of rumen microorganisms, increase the content of sugar precursors (propionic acid), and finally increase blood sugar levels. The addition of rumen choline chloride has more functions of redistribution of energy substances in the body and liver protection, without adding extra energy to the body. Adding glycerol can provide extra energy for the body, but the added glycerin can only play a role if it is effectively used by the liver for gluconeogenesis. When the energy negative balance is serious and the liver has a lot of fat accumulation, the liver gluconeogenesis function is damaged, and adding glycerol cannot Get the best out of it. Therefore, adding glycerol and rumen-passed choline chloride at the same time, on the one hand, adding glycerol can provide extra energy for the body; on the other hand, adding rumen-passed choline chloride can redistribute energy substances in the body and transfer The accumulated fatty acid is transported out to maintain the normal liver gluconeogenesis function, which improves the sugar production efficiency of glycerol, so that the simultaneous addition of the two has a synergistic effect.

Description of drawings

Fig. 1 is the relationship diagram of the influence of glycerol and rumen-passed choline chloride on body weight gain of restricted-feeding Hu sheep;

Figure 2 is a graph showing the influence of glycerol and rumen-passed choline chloride on blood β-hydroxybutyric acid concentration of restricted-feeding Hu sheep;

Fig. 3 is a diagram showing the influence of glycerol and rumen-passed choline chloride on blood biochemical indexes of restricted-feeding Hu sheep.

Detailed ways

The present invention will be further described below in conjunction with drawings and embodiments.

The raw materials and instruments used in the present invention are all commercially available.

Rumen-passed choline chloride was purchased from Hangzhou Kangdequan Feed Co., Ltd. Glycerin was purchased from Shanghai Jiujie Industrial Co., Ltd.

Portable pH meter (HI 9024; HAMA Instruments, USA).

Blood ketone meters and blood ketone test strips were purchased from Abbott Trading (Shanghai) Co., Ltd. Kits for plasma glucose, free fatty acids, triglycerides, total cholesterol, high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein were purchased from Nanjing Jiancheng Bioengineering Institute.

Gas chromatograph (GB-2014; Shimadzu Instruments, Japan).

Example 1

In the experiment, 24 Hu sheep with good body condition and the same parity were selected, and the estrus was in the same period and the gestation period was 101 days. Randomly divided into control group (feeding 30% of normal feeding amount), glycerol group (feeding 30% of normal feeding amount+40ml/glycerol/day), rumen-passed choline chloride group (feeding normal feeding amount) 30%+10g/body/day of rumen chloride choline) and glycerol+rumen choline chloride group (30%+40ml/day glycerol+10g//day of normal feeding amount). rumen choline chloride). During the test period, the corresponding additives of each group were mixed with a small amount of concentrate every day, and the remaining feed was fed after the Hu sheep had eaten. During the experiment, Hu sheep were fed in a single pen and had free access to water. The composition and nutrient composition of the diet are shown in Table 1.

Table 1 Test diet composition and nutritional level

Example 2

During the test, the initial and end body weights of the Hu sheep in each group were measured, and the body weight gain was calculated. The results are shown in Figure 1.

As shown in Figure 1, glycerol supplementation, and concurrent glycerol and rumen-passed choline chloride, reduced weight loss induced by feeding restriction compared with controls. Adding rumen choline chloride had no significant effect on weight loss caused by feed restriction. The above results indicated that the addition of glycerol and the simultaneous addition of glycerol and rumen-passed choline chloride can provide energy for the body, thereby reducing the mobilization of body reserves.

Example 3

Take the Hu sheep of Example 1, collect jugular vein blood before morning feeding on the 0, 3, 6 and 9 days of the test period, and immediately measure the blood β-hydroxybutyric acid concentration with a blood ketone meter and a blood ketone test strip. The result is shown in Figure 2.

As shown in Figure 2, the blood β-hydroxybutyric acid concentration continued to increase in the control group. Supplementation of glycerol and rumen-passed choline chloride prevented feed restriction-induced increases in blood β-hydroxybutyric acid concentrations compared with controls. The above results indicated that the addition of glycerol and rumen-passed choline chloride could prevent excessive mobilization of body fat.

Example 4

Take the Hu sheep of Example 1, collect jugular vein blood before morning feeding on the 0th and 9th days of the test period, then centrifuge at 1000g 4°C for 15 minutes, take the supernatant, and immediately freeze it in a -20°C refrigerator for blood Biochemical index detection. On the 9th day of the test period, the rumen fluid was collected 4 hours after feeding with an oral rumen fluid collection device, and the rumen pH was measured immediately. After the pH value was measured, the rumen contents were filtered through four layers of gauze to collect the rumen fluid, stored at -20°C, and prepared to measure the concentration of volatile fatty acids.

Example 5

Volatile fatty acids were measured on the rumen fluid of Example 4, and the effects of glycerin and rumen-passed choline chloride on rumen fermentation parameters were investigated. The results are shown in Table 2.

Table 2 Effects of adding glycerol and rumen-passed choline chloride on rumen fermentation parameters

As shown in Table 2, compared with the control group, the addition of glycerol and the simultaneous addition of glycerol and rumen-passed choline chloride significantly decreased the rumen pH, significantly increased the concentration of propionic acid, and significantly decreased the ratio of ethyl to propylene. Addition of glycerol and rumen-passed choline chloride tended to increase total VFA. Simultaneous addition of glycerol and rumen-passed choline chloride significantly increased butyric and valeric acid concentrations compared to the control group. The above results indicated that the addition of glycerol could ferment propionate in the rumen. Therefore, the addition of glycerol significantly decreased the ratio of ethyl to propylene in rumen fluid, significantly decreased the pH value of rumen fluid, and had a tendency to increase the level of total VFA. In addition, compared with the other three groups, the concentration of propionic acid in the rumen fluid was the highest in the group that added glycerol and rumen-passed choline chloride at the same time, indicating that the addition of rumen-passed choline chloride can increase the activity of rumen microorganisms and improve the fermentation efficiency.

Example 6

Blood biochemical indicators were measured on the blood of Example 4, and the effects of glycerin and rumen-passed choline chloride on blood biochemical indicators were investigated. The results are shown in FIG. 3 .

Kits for plasma glucose, free fatty acids, triglycerides, total cholesterol, high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein were purchased from Nanjing Jiancheng Bioengineering Institute.

As shown in Figure 3, compared with the control group, the addition of glycerol and rumen-passed choline chloride can both increase blood glucose levels, and the simultaneous addition of glycerol and rumen-passed choline chloride has the greatest effect. Compared with the control group, the addition of rumen-treated choline chloride significantly increased the levels of free fatty acids and very low-density lipoprotein in blood. The above results show that the addition of glycerin and rumen-passed choline chloride can both increase blood glucose levels, but their mechanisms of action are different. From the results in Example 5, it can be seen that glycerol reduces the ratio of ethyl to propylene in rumen fluid and increases the ratio of propionic acid, while Acid is the main precursor of gluconeogenesis to glucose in ruminants. Rumen-passed choline chloride can also increase blood glucose levels, and increase blood levels of free fatty acids and very low-density lipoproteins, suggesting that rumen-passed choline chloride can transport excess triglycerides deposited in the liver out of the liver to protect liver function. The reason why the rumen-passed choline chloride improves the blood glucose level is achieved by protecting the liver function, thereby improving the ability of liver gluconeogenesis. The effect of adding glycerol alone is not as good as adding glycerol and rumen-passed choline chloride at the same time. The main reason is that adding glycerol alone only increases the glycogenic precursors, but the hepatic gluconeogenesis function decreases due to excessive deposition of fatty acids in the liver, while adding too much choline chloride at the same time Rumen choline chloride can transport fatty acids in the liver to improve liver gluconeogenesis. Adding rumen-passed choline chloride alone can improve the gluconeogenesis function of the liver, but due to the lack of sufficient glucose precursors, the glucose produced by gluconeogenesis is not as much as adding glycerin and rumen-passed choline chloride at the same time. . Therefore, adding both glycerol and rumen-passed choline chloride is more effective than adding either of them alone.

Claims (7)

1. glycerol and rumen bypass becholine are in preparation prevention and/or treatment sheep eclampsia feed addictive or drug In application.

2. application according to claim 1, which is characterized in that the additive is the additive of feedstuff for hu yang.

3. application according to claim 2, which is characterized in that the feed is used for sheep latter half of gestation.

4. application according to claim 1 to 3, which is characterized in that the sheep is to cherish more lamb pregnancy ewes.

5. application according to claim 1, which is characterized in that the feed addictive or drug dose are preferably glycerol Additive amount is 40ml/ pcs/day, and rumen bypass becholine additive amount is 10g/ pcs/day.

6. application according to claim 1, which is characterized in that the glycerol is by improving volatile fatty acid in rumen fluid Concentration, improve blood glucose levels, reduce in blood beta-hydroxybutyric acid concentration and rumen bypass becholine by by liver Extra free-fat acid transporter goes out liver in dirty, and is utilized by other tissues, and blood glucose levels are improved.

7. one kind is for preventing and/or treating sheep eclampsia feed addictive or pharmaceutical composition, which is characterized in that Including glycerol and rumen bypass becholine as auxiliary material needed for effective component and feed addictive or pharmaceutical composition.