CN110477086B - Loligo chinensis Gray biogenic amine inhibitor, preparation method and storage method for inhibiting squid biogenic amine - Google Patents

Abstract

The invention belongs to the field of aquatic product quality and safety control, and specifically relates to a squid biogenic amine inhibitor, preparation and storage methods for inhibiting squid biogenic amines. The composite biogenic amine inhibitor includes: component A and component B, which are used in combination; the component A includes thymol, ε-polylysine hydrochloride, asafoetida Acid and solvent A; the B component includes purslane extract, tangerine peel extract and solvent B. The ingredients contained in the compound biogenic amine inhibitor are all natural food grade and are highly safe. They can be used to control biogenic amines in seafood, especially squid. The processed products have good eating quality and good taste. The compound biogenic amine inhibitor not only interferes with the information transmission between microorganisms, but also has a bacteriostatic effect; the purslane extract therein has the dual effect of inhibiting squid biogenic amines and formaldehyde.

Description

Squid biogenic amine inhibitors, preparation and storage methods for inhibiting squid biogenic amines

Technical field

The invention belongs to the field of aquatic product quality and safety control, and specifically relates to a squid biogenic amine inhibitor, preparation and storage methods for inhibiting squid biogenic amines.

Background technique

Squid, scientific name is Squid, class Cephalopoda, family Squid. It is the common name for molluscs in the Phylum Mollusca, Class Cephalopoda, Class Colletozoa, Molluscidae, and Order Psiluriformes. Squid is a high-protein and low-fat aquatic product. It is rich in various amino acids necessary for the human body. It is also rich in flavor substances such as taurine, carnitine, purine, and nucleotides. It is a nutritional and health-care type with good flavor. aquatic product resources. At present, the widely used storage method is low-temperature freezing. Squid is greatly affected by the freezing temperature. Fish protein is prone to freeze denaturation, resulting in deterioration of the tenderness, water-holding capacity, gelling and nutritional value of the fish. . Squid will also produce a large amount of biogenic amines during storage. Excessive biogenic amines can cause poisoning in the human body and cause serious reactions, such as headaches, blood pressure changes, respiratory disorders, heart palpitations and vomiting. Therefore, it is of great significance to find a practical method to control the production of biogenic amines in squid, delay its spoilage and reduce the economic losses of aquatic enterprises.

Biogenic amines are stable in nature. How to control the content of biogenic amines in squid is a key and difficult issue in the field of aquatic product safety. It has been reported that the control methods of biogenic amines in food include ultrahigh pressure, irradiation and microbial methods. These methods have high costs. , high operating requirements, insignificant effects or reduced food quality, etc., it is currently difficult to popularize and apply. At the same time, different food compositions are different, and the control methods of biogenic amines are also different. Therefore, it is of great significance to develop efficient, low-cost, and easy-to-operate biogenic amine control technology in squid.

Contents of the invention

In order to achieve the above purpose, the present invention proposes a squid biogenic amine inhibitor, a preparation method and a storage method for inhibiting squid biogenic amines. The storage method is aimed at the current problem of excessive biogenic amines in squid. The compound biogenic amine inhibitor has high efficiency and low cost. The advantages of low cost and easy operation improve the eating safety of squid.

The technical solution of the present invention is:

A squid compound biogenic amine inhibitor, the compound biogenic amine inhibitor includes: component A and component B, and the component A and component B are used in combination;

The A component includes thymol, ε-polylysine hydrochloride, ferulic acid and solvent A;

The B component includes purslane extract, tangerine peel extract and solvent B.

Further, the mass fraction of each component in the A component is: thymol 0.01% to 0.1%, ε-polylysine hydrochloride 0.01% to 0.1%, ferulic acid 0.01% to 0.1%, the remainder The amount is solvent A.

Further, the mass fraction of each component in component B is: 0.05% to 0.5% of purslane extract, 0.05% to 0.5% of tangerine peel extract, and the balance is solvent B.

Further, the solvent A is water, or a mixture of water and ethanol.

Further, the solvent B is water.

Furthermore, the thymol was mainly derived from nature in the early days and is widely present in plants. Currently, thymol can be chemically synthesized; thymol has antiseptic properties and medicinal value. It also has antifungal and parasitic functions and can be used to treat wounds. , storage of anatomical specimens, etc.; in addition, thymol can also be used as an antioxidant;

The ε-polylysine hydrochloride is a polypeptide polymer with antibacterial effect. It is natural, water-soluble, non-toxic to humans and the environment, stable at high temperatures, and is a safe biological Preservative, can be decomposed into lysine. ε-Polylysine hydrochloride has antibacterial effects and has obvious antibacterial effects against Bacillus cereus;

The ferulic acid is a phenolic acid substance widely present in plants. Its chemical name is 3-methoxy-4-hydroxycinnamic acid, its molecular formula is C ₁₀ H ₁₀ O ₄ , and its relative molecular mass is 194.19. Ferulic acid has a series of functions such as treating cardiovascular and cerebrovascular diseases, lowering blood pressure and blood lipids, preventing and treating coronary heart disease, protecting liver and lungs, and anti-aging;

The purslane extract has the dual effect of inhibiting squid biogenic amines and formaldehyde;

The tangerine peel can be used as medicine and food, and the tangerine peel extract has antibacterial effects.

Furthermore, the precursor substances of biogenic amines are generally amino acids. Through corresponding pathways, amino acids are metabolized to form biogenic amines; the main production pathway of biogenic amines is from free amino acids through decarboxylation of microorganisms or through decarboxylation of amino acid decarboxylase. ; The formation of biogenic amines generally requires three prerequisites: (1) the existence of free amino acids; (2) the existence of microorganisms that produce decarboxylase and the conditions for the survival and reproduction of microorganisms; (3) the conditions for the activity of decarboxylase; by reducing the The content of biogenic amines can be effectively controlled by reducing the content of free amino acids, reducing the reproduction of microorganisms or reducing the activity of decarboxylase; the biogenic amine inhibitor of the present invention is a comprehensive control of the above-mentioned conditions for producing biogenic amines.

Furthermore, the complex biogenic amine inhibitor not only interferes with the information transmission between microorganisms in squid, but also has a bacteriostatic effect.

Further, the squid is Argentine squid.

Another object of the present invention is to provide a method for preparing a squid compound biogenic amine inhibitor. The preparation method includes the following steps:

Step 1, preparation of purslane extract: dry purslane is crushed and sieved, and the purslane powder is extracted with ethanol aqueous solution, assisted by ultrasonic waves; the extract is filtered and purified (purified using AB-8 macroporous resin) , elute with aqueous ethanol solution, concentrate in vacuum, and freeze-dry to obtain purslane extract;

Step 2, preparation of tangerine peel extract: dry tangerine peel is crushed and sieved, and the tangerine peel powder is extracted with ethanol aqueous solution, assisted by microwave; the extract is filtered, concentrated in vacuum, purified (purified with HPD-600 resin), and eluted with ethanol aqueous solution. , vacuum concentration and freeze-drying to obtain tangerine peel extract;

Step 3, preparation of component A: Completely dissolve thymol with ethanol (a small amount), add ε-polylysine hydrochloride and ferulic acid in proportion, add water, and stir until completely dissolved to obtain the A components;

Step 4, preparation of component B: Dissolve purslane extract and tangerine peel extract with water to obtain component B.

Further, when the purslane powder is extracted with ethanol aqueous solution in step 1, the mass fraction of ethanol is 30%, the solid-liquid ratio is 1:30; the ultrasonic time is 30 min, and the temperature is 45°C.

Further, the mass fraction of ethanol in the aqueous ethanol solution used for elution in step 1 is 30%.

Further, when the tangerine peel powder is extracted with ethanol aqueous solution in step 2, the mass fraction of ethanol is 50%, the solid-liquid ratio is 1:25; the microwave power is 200W, the microwave extraction time is 10min, and the temperature is 50°C.

Further, the mass fraction of ethanol in the aqueous ethanol solution used for elution in step 2 is 50%.

Another object of the present invention is to provide a storage method for inhibiting biogenic amines in squid. The storage method uses the above-mentioned composite biogenic amine inhibitor to inhibit biogenic amines in squid during storage; the storage method includes the following steps:

1) Cleaning: Remove the internal organs from the fresh squid and wash it;

2) Bacteria reduction treatment: Sterilize the cleaned squid by using ultraviolet light;

3) Compound biogenic amine inhibitor impregnation: place the squid after bacteria reduction treatment into the component A and then soak the squid into the component B;

4) Drain, pack and store: Drain the surface water of the squid that has been impregnated with the biogenic amine inhibitor, seal it and store it. It can be sealed in a sterile retort bag.

Further, the ultraviolet light irradiation time is 5 to 10 minutes.

Further, the soaking time is 10 to 30 minutes respectively.

Further, the storage temperature of squid is -2~4℃.

The present invention has the following beneficial technical effects:

The ingredients contained in the squid compound biogenic amine inhibition of the present invention are all natural food grade and are highly safe. They can be used to control biogenic amines in seafood, especially squid. The processed products have good eating quality and good taste.

The biogenic amine inhibitor of the present invention has a synergistic effect.

The biogenic amine inhibitor component A of the present invention has a relatively strong antibacterial effect and can inhibit the production of biogenic amines. The biogenic amine inhibitor component B has a strong antioxidant effect and at the same time has a strong antibacterial effect on some nitrogen-containing compounds (amine compounds). The scavenging effect, when used together, can greatly reduce the biogenic amine content in squid, while maintaining the storage quality of squid and extending the shelf life of squid.

The squid compound biogenic amine inhibitor of the present invention not only interferes with the information transmission between microorganisms, but also has a bacteriostatic effect.

The purslane extract in the squid compound biogenic amine inhibitor of the present invention has the dual effect of inhibiting squid biogenic amines and formaldehyde.

The preparation method of the squid biogenic amine inhibitor of the present invention is simple and convenient to use. It only requires dipping the squid in the biogenic amine inhibitor at low temperature.

Detailed ways

In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention will be described in further detail below in conjunction with comparative examples, examples and experimental data lists. It should be understood that the specific embodiments described here are only used to explain the present invention and are not intended to limit the present invention.

On the contrary, the invention covers any alternatives, modifications, equivalent teaching methods and solutions that fall within the spirit and scope of the invention as defined by the claims. Furthermore, in order to enable the public to have a better understanding of the present invention, some specific details are described in detail in the following detailed description of the present invention. It is possible for a person skilled in the art to fully understand the present invention without these detailed descriptions.

It should be clear that the described embodiments are only some, but not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

The terminology used in the embodiments of the present invention is only for the purpose of describing specific embodiments and is not intended to limit the present invention. As used in this embodiment and the appended claims, the singular forms "a," "the" and "the" are intended to include the plural forms as well, unless the context clearly dictates otherwise.

Comparative example 1

In this comparative example, a thymol solution is used to inhibit biogenic amines in squid. The thymol is completely dissolved with a small amount of ethanol, and then water is added to obtain a thymol solution. The mass fraction of thymol in the solution is 0.05%.

This comparative example also provides a storage method for inhibiting squid biogenic amines, which method includes the following steps:

(1) Cleaning: Remove the internal organs from the fresh squid, wash and slice it;

(2) Bacteria reduction treatment: Place the squid slices under ultraviolet light for 5 minutes;

(3) Biogenic amine inhibitor dipping: soak the squid after bacteria reduction treatment in thymol solution for 20 minutes;

(4) Drainage packaging: Drain the surface water of the squid impregnated with the biogenic amine inhibitor, put it into a sterile cooking bag, seal it, and store it at 0°C.

Comparative example 2

In this comparative example, a medium ε-polylysine hydrochloride solution is used to inhibit biogenic amines in squid. The ε-polylysine hydrochloride is dissolved in water to obtain an ε-polylysine hydrochloride solution. The solution The mass fraction of ε-polylysine hydrochloride is 0.05%.

A storage method for inhibiting squid biogenic amines in this comparative example is basically the same as that in comparative example 1, with the only difference being:

In (3), the squid after bacteria reduction treatment was immersed in ε-polylysine hydrochloride solution for 20 minutes.

Comparative example 3

In this comparative example, a mixed solution of thymol and ε-polylysine hydrochloride is used to inhibit biogenic amines in squid, and water and a small amount of ethanol are used as solvents to dissolve; the solution of thymol and ε-polylysine hydrochloride is obtained. Mixed solution, in which the mass fraction of thymol is 0.025% and the mass fraction of ε-polylysine hydrochloride is 0.025%.

A storage method for inhibiting squid biogenic amines in this comparative example is basically the same as that in comparative example 1, with the only difference being:

In (3), the bacteria-reduced squid is immersed in a mixed solution of thymol and ε-polylysine hydrochloride for 20 minutes.

Comparative example 4

This comparative example uses purslane extract solution to inhibit biogenic amines in squid.

Among them, the preparation method of the purslane extract is: dry purslane is crushed and sieved, and the purslane powder is extracted with 30% ethanol aqueous solution, assisted by ultrasonic waves, the ultrasonic time is 30 minutes, and the liquid-to-solid ratio is 30:1. The temperature is 45°C. The extract is filtered, purified with AB-8 macroporous resin, eluted with 30% ethanol aqueous solution, concentrated in vacuum, and freeze-dried to obtain the purslane extract.

The purslane extract powder is dissolved in water to obtain a purslane extract solution, and the mass fraction of the purslane extract in the solution is 0.2%.

A storage method for inhibiting squid biogenic amines in this comparative example is basically the same as that in comparative example 1, with the only difference being:

In (3), the squid after bacteria reduction treatment was soaked in the purslane extract solution for 20 minutes.

Comparative example 5

This comparative example uses tangerine peel extract solution to inhibit biogenic amines in squid.

Among them, the preparation method of tangerine peel extract is: dry tangerine peel is crushed and sieved, and the tangerine peel powder is extracted with 50% ethanol aqueous solution, microwave-assisted, microwave power 200W, microwave extraction time 10min, liquid-to-solid ratio 25:1, temperature The temperature is 50°C, filter the extract, concentrate in vacuum, purify with HPD-600 resin, elute with 50% ethanol aqueous solution, concentrate in vacuum, and freeze-dry to obtain tangerine peel extract.

The tangerine peel extract powder is dissolved in water to obtain an tangerine peel extract solution, and the mass fraction of the tangerine peel extract in the solution is 0.2%.

A storage method for inhibiting squid biogenic amines in this comparative example is basically the same as that in comparative example 1, with the only difference being:

In (3), the squid after bacteria reduction treatment was soaked in the tangerine peel extract solution for 20 minutes.

Comparative example 6

This comparative example uses a mixed solution of purslane extract and tangerine peel extract to inhibit biogenic amines in squid.

Among them, the preparation method of the purslane extract is: dry purslane is crushed and sieved, and the purslane powder is extracted with 30% ethanol aqueous solution, assisted by ultrasonic waves, the ultrasonic time is 30 minutes, and the liquid-to-solid ratio is 30:1. The temperature is 45°C. The extract is filtered, purified with AB-8 macroporous resin, eluted with 30% ethanol aqueous solution, concentrated in vacuum, and freeze-dried to obtain the purslane extract.

The preparation method of tangerine peel extract is as follows: dry tangerine peel is crushed and sieved, and the tangerine peel powder is extracted with 50% ethanol aqueous solution, microwave-assisted, microwave power 200W, microwave extraction time 10min, liquid-to-solid ratio 25:1, temperature 50 ℃, filter the extract, concentrate in vacuum, purify with HPD-600 resin, elute with 50% ethanol aqueous solution, concentrate in vacuum, and freeze-dry to obtain tangerine peel extract.

The purslane extract powder and the tangerine peel extract powder are dissolved in water. The mass fraction of the purslane extract in the solution is 0.1%, and the mass fraction of the tangerine peel extract in the solution is 0.1%.

A storage method for inhibiting squid biogenic amines in this comparative example is basically the same as that in comparative example 1, with the only difference being:

In (3), the bacteria-reduced squid is soaked in a mixed solution of purslane extract and tangerine peel extract for 20 minutes.

Example 1

This embodiment proposes a squid compound biogenic amine inhibitor. The compound biogenic amine inhibitor includes: component A and component B, and the component A and component B are used in combination;

The A component includes thymol, ε-polylysine hydrochloride and ferulic acid;

The B component includes purslane extract and tangerine peel extract.

The preparation of purslane extract and tangerine peel extract in component B is the same as Comparative Example 6.

The preparation method of component A is: completely dissolve thymol with a small amount of ethanol, add ε-polylysine hydrochloride and ferulic acid in sequence in proportion, add water, stir until completely dissolved, and then add the remaining water to obtain The A component. The mass fraction of each component of component A is: thymol 0.01%, ε-polylysine hydrochloride 0.01%, ferulic acid 0.01%, and the balance is water and ethanol.

The preparation method of component B is: dissolve the purslane extract and the tangerine peel extract in water, so that the mass fraction of the purslane extract is 0.05% and the mass fraction of the tangerine peel extract is 0.05%.

This embodiment also provides a storage method for inhibiting biogenic amines in squid. The storage method uses the above-mentioned composite biogenic amine inhibitor to inhibit biogenic amines in squid during storage; the storage method includes the following steps:

1) Cleaning: Remove the internal organs from the fresh squid and wash it;

2) Bacteria reduction treatment: irradiate the cleaned squid with ultraviolet light for 5 minutes;

3) Impregnation: Place the squid after bacteria reduction treatment in the component A and soak it for 10 minutes, and then place the squid in the component B and soak it for 10 minutes;

4) Drain, pack and store: Drain the surface water of the squid that has been impregnated with the biogenic amine inhibitor, put it into a sterile cooking bag, seal it and store it at 0°C.

Example 2

The composition and preparation method of a squid compound biogenic amine inhibitor in this embodiment are basically the same as those in Embodiment 1, except that:

The mass fraction of each component in component A is: 0.05% thymol, 0.05% ε-polylysine hydrochloride, 0.05% ferulic acid, and the balance is water and ethanol.

The mass fraction of each component in component B is: 0.25% purslane extract, 0.25% tangerine peel extract, and the balance is water.

This example provides a storage method for inhibiting squid biogenic amines, which is basically the same as Example 1, except that:

2) The cleaned squid will be irradiated with ultraviolet light for 7 minutes;

3) In 3), place the squid that has been treated with bacteria reduction and soak it in the component A for 20 minutes, and then place the squid in the component B and soak it for 20 minutes.

Example 3

The composition and preparation method of a squid compound biogenic amine inhibitor in this embodiment are basically the same as those in Embodiment 1, except that:

The mass fraction of each component in component A is: thymol 0.1%, ε-polylysine hydrochloride 0.1%, ferulic acid 0.1%, a small amount of ethanol, and the balance is water.

The mass fraction of each component in component B is: 0.5% of purslane extract and 0.5% of tangerine peel extract.

This example provides a storage method for inhibiting squid biogenic amines, which is basically the same as Example 1, except that:

2) Irradiate the cleaned squid with ultraviolet light for 10 minutes;

3) In 3), place the squid that has been treated with bacteria reduction and soak it in the component A for 30 minutes, and then place the squid in the component B and soak it for 30 minutes.

Example 4

The composition and preparation method of a squid compound biogenic amine inhibitor in this embodiment are basically the same as those in Example 1, except that the mass fraction of each component in component A is: thymol 0.05%, ε-polymer Acid hydrochloride 0.05%, ferulic acid 0.05%, the balance is water and ethanol;

The mass fraction of each component in component B is: 0.25% purslane extract, 0.25% tangerine peel extract, and the balance is water.

This example provides a storage method for inhibiting squid biogenic amines, which is basically the same as Example 1, except that:

2) The cleaned squid will be irradiated with ultraviolet light for 7 minutes;

3) In the process, the squid after the bacteria reduction treatment is placed in the component A and soaked for 20 minutes, and then the squid is placed in the component B and soaked for 20 minutes;

4) Drain the surface water of the squid impregnated with the biogenic amine inhibitor, put it into a sterile cooking bag, seal it and store it at -2°C.

Example 5

The composition and preparation method of a squid compound biogenic amine inhibitor in this embodiment are basically the same as those in Example 1, except that the mass fraction of each component in component A is: thymol 0.05%, ε-polymer Acid hydrochloride 0.05%, ferulic acid 0.05%, the balance is water and ethanol.

The mass fraction of each component in component B is: 0.25% purslane extract, 0.25% tangerine peel extract, and the balance is water.

This example provides a storage method for inhibiting squid biogenic amines, which is basically the same as Example 1, except that:

2) The cleaned squid will be irradiated with ultraviolet light for 7 minutes;

3) In the process, the squid after the bacteria reduction treatment is placed in the component A and soaked for 20 minutes, and then the squid is placed in the component B and soaked for 20 minutes;

4) Drain the surface water of the squid impregnated with the biogenic amine inhibitor, put it into a sterile cooking bag, seal it and store it at 4°C.

Through the above-mentioned Comparative Examples 1 to 6 and Examples 1 to 5, the changes in the volatile basic nitrogen (TVB-N) content, the changes in the putrescine content and the changes in the cadaverine content of the squid in the control group were detected during the storage period of the squid. , the changes are shown in Tables 1 to 3 below.

Table 1 Changes in volatile base nitrogen (TVB-N) content of squid during storage (mg/100g)

Table 2 Changes in putrescine content in squid during storage (mg/kg)

Table 3 Changes in cadaverine content of squid during storage (mg/kg)

Analysis of Table 1 shows that the TVB-N content of the squid treated with the compound biogenic amine inhibitor of the present invention is lower than that of the control group, indicating that the compound biogenic amine inhibitor of the present invention inhibits the production of biogenic amines in squid.

Analysis of Tables 1 to 3 shows that the TVB-N content, putrescine and cadaverine contents of Comparative Example 2 are lower than those of Comparative Example 1, indicating that the effect of ε-polylysine hydrochloride is better than that of thymol. The effect is better than that of Comparative Examples 1 and 2. The effect of ε-polylysine hydrochloride and thymol in inhibiting biogenic amines is better than the effect of the two acting alone. There is a synergistic effect between the two; TVB of Comparative Example 4 The -N content is lower than Comparative Example 5, indicating that the effect of purslane extract is better than that of tangerine peel extract. The combined use of the two in inhibiting biogenic amines in squid is much better than the effect of using alone, and there is a synergistic effect between the two. The effect of Example 1 is better than that of Comparative Examples 3 and 6, indicating that the combined use of biogenic amine inhibitor one and biogenic amine inhibitor two is far better than the effect of separate use. Examples 2 and 3 are better than Example 1, which illustrates the effect of the dosage of the biogenic amine inhibitor on inhibiting biogenic amines. The TVB-N content, putrescine and cadaverine contents of Example 4 are lower than the TVB-N content, putrescine and cadaverine contents of Example 5, indicating that low temperature is beneficial to controlling the content of biogenic amines.

The above is a detailed introduction to the squid biogenic amine inhibitor, preparation and storage method for inhibiting squid biogenic amines provided by the embodiments of the present invention. The description of the above embodiments is only used to help understand the method and its core idea of the present invention; at the same time, for those of ordinary skill in the art, there will be changes in the specific implementation and application scope based on the idea of the present invention. In summary, the contents of this description should not be construed as limitations of the present invention.

For example, certain words are used in the description and claims to refer to specific components. Those skilled in the art will understand that hardware manufacturers may use different names to refer to the same component. This specification and the claims do not use differences in names as a means to distinguish components; rather, differences in functions of the components serve as a criterion for distinction. For example, the words "include" and "include" mentioned in the entire description and claims are open-ended terms, so they should be interpreted as "includes/includes but is not limited to." "Approximately" means that within an acceptable error range, those skilled in the art can solve the technical problem within a certain error range and basically achieve the technical effect. The following descriptions of the specification are preferred embodiments for implementing the present invention. However, the descriptions are for the purpose of illustrating the general principles of the present invention and are not intended to limit the scope of the present invention. The protection scope of the present invention shall be determined by the appended claims.

It should also be noted that the terms "includes", "includes" or any other variation thereof are intended to cover a non-exclusive inclusion, such that a good or system including a list of elements includes not only those elements but also those not expressly listed other elements, or elements inherent to the product or system. Without further limitation, an element defined by the statement "comprises a..." does not exclude the presence of other identical elements in the goods or systems that include the stated element.

It should be understood that the term "and/or" used in this article is only an association relationship describing related objects, indicating that there can be three relationships, for example, A and/or B, which can mean: A alone exists, and A and A exist simultaneously. B, there are three situations of B alone. In addition, the character "/" in this article generally indicates that the related objects are an "or" relationship.

The above description shows and describes several preferred embodiments of the present invention, but as mentioned above, it should be understood that the present invention is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various embodiments. Other combinations, modifications and environments, and can be modified through the above teachings or technology or knowledge in related fields within the scope of the application concept described herein. Any modifications and changes made by those skilled in the art that do not depart from the spirit and scope of the present invention shall be within the protection scope of the appended claims of the present invention.

Claims (7)

1. A squid compound biogenic amine inhibitor, characterized in that the compound biogenic amine inhibitor includes: A component and B component, and the A component and B component are used in combination; The A component includes thymol, ε-polylysine hydrochloride, ferulic acid and solvent A; The B component includes purslane extract, tangerine peel extract and solvent B; The mass fraction of each component in component A is: thymol 0.01% to 0.1%, ε-polylysine hydrochloride 0.01% to 0.1%, ferulic acid 0.01% to 0.1%, and the balance is solvent A; The mass fraction of each component in component B is: 0.05% to 0.5% of purslane extract, 0.05% to 0.5% of tangerine peel extract, and the balance is solvent B. 2. A squid compound biogenic amine inhibitor according to claim 1, characterized in that the squid is Argentine squid. 3. A kind of preparation method of squid compound biogenic amine inhibitor as claimed in claim 1 or 2, characterized in that, the preparation method includes the following steps: Step 1, preparation of purslane extract: dry purslane is crushed and sieved, and the purslane powder is extracted with ethanol aqueous solution, assisted by ultrasonic wave; the extract is filtered, purified, eluted with ethanol aqueous solution, concentrated in vacuum, and frozen After drying, purslane extract is obtained; Step 2, preparation of tangerine peel extract: dry tangerine peel is crushed and sieved, and the tangerine peel powder is extracted with ethanol aqueous solution, assisted by microwave; the extract is filtered, vacuum concentrated, purified, eluted with ethanol aqueous solution, vacuum concentrated, and freeze-dried, that is Obtain tangerine peel extract; Step 3, preparation of component A: completely dissolve thymol in ethanol, add ε-polylysine hydrochloride and ferulic acid in proportion, add water, stir until completely dissolved, and obtain component A; Step 4, preparation of component B: Dissolve purslane extract and tangerine peel extract with water to obtain component B. 4. The preparation method according to claim 3, characterized in that when the purslane powder is extracted with ethanol aqueous solution in step 1, the mass fraction of ethanol is 30%, the solid-liquid ratio is 1:30; the ultrasonic time is 30min, temperature is 45℃; The mass fraction of ethanol in the aqueous ethanol solution used for elution in step 1 is 30%. 5. The preparation method according to claim 3, characterized in that when the dried tangerine peel powder is extracted with ethanol aqueous solution in step 2, the mass fraction of ethanol is 50%, and the solid-liquid ratio is 1:25; the microwave power is 200W, and the microwave power is 200W. The extraction time is 10 minutes and the temperature is 50°C; The mass fraction of ethanol in the aqueous ethanol solution used for elution in step 2 is 50%. 6. A storage method for inhibiting biogenic amines in squid, characterized in that the storage method uses a compound biogenic amine inhibitor as claimed in claim 1 or 2 to inhibit biogenic amines in squid during storage; the storage method includes Follow these steps: 1) Cleaning: Remove the internal organs from the fresh squid and wash it; 2) Bacteria reduction treatment: sterilize the cleaned squid; 3) Compound biogenic amine inhibitor impregnation: Dip the squid that has been treated with bacteria reduction in the component A, and then impregnate the squid in the component B; 4) Drain, pack and store: Drain the surface water of the Argentine squid impregnated with biogenic amine inhibitors, seal it and store it. 7. The storage method according to claim 6, characterized in that the sterilization method in step 2) adopts ultraviolet light irradiation; The ultraviolet light irradiation time is 5 to 10 minutes; The immersion time of squid in component A is 10 to 30 minutes; The immersion time of squid in component B is 10 to 30 minutes; The storage temperature of squid is -2~4℃.