CN110464756A - It is a kind of for adjusting the gegen qinlian decoction water extract of fat body - Google Patents

Abstract

The invention relates to the technical field of traditional Chinese medicine, and specifically discloses a Gegen Qinlian Decoction water extract used for regulating an obese body. The Gegen Qinlian Decoction water extract is obtained from Pueraria lobata, Scutellaria baicalensis, Rhizoma Coptidis, and licorice root through medicinal material compatibility and water decocting; the medicinal material compatibility is calculated in parts by mass as: 7-9 parts of Pueraria Root, 2-4 parts of Scutellaria baicalensis 2-4 parts of Coptis Rhizoma, and 1-3 parts of Zhigancao; the Gegen Qinlian Decoction water extract is used to prepare medicines for regulating the intestinal flora and liver biological clock genes of the damp-heat intestinal tract of obese body, and has the ability to reverse Akkermansia and Lactobacillus bacteria. At the same time, it can up-regulate the expression and transcription levels of the liver Clock and Arntl genes, which can effectively reduce the level of serum triglycerides in the body and regulate other blood lipids and blood sugar indicators such as total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, blood sugar, insulin and so on. It also has a regulatory effect on body metabolites such as short-chain, medium-chain and long-chain fatty acids, tryptophan, kynuric acid and cannabinoids.

Description

A kind of Gegen Qinlian decoction water extract for regulating obese body

technical field

The invention belongs to the technical field of traditional Chinese medicine, in particular to a water extract of Gegen Qinlian Decoction for regulating obese body, in particular to a kind of intestinal flora Akkermansia , Lactobacillus and liver circadian clock gene for regulating dampness and heat of intestinal tract of obese body Clock , Arntl 's Gegen Qinlian Soup Water Extract.

Background technique

From single-celled bacteria to plants and animals, nearly all organisms exhibit behavioral, physiological, and biochemical rhythms that approximate the timing of the Earth's rotation, the approximately 24-hour circadian rhythm. The biological clock is mainly regulated by the surrounding environment and other signal stimuli, such as light, food intake, temperature, humidity, etc. In higher multicellular organisms, the biological clock can be divided into a mother clock (main biological clock) and a daughter clock (peripheral biological clock). The physiological system of mammals is mainly composed of a central pacemaker, called the main biological clock, located in the hypothalamus and optic chiasm. Nucleus (SCN) region, mainly entrained by light; sub-clocks are located within cells of peripheral tissues (lung, heart, pancreas, adipose tissue, etc.). Biological rhythms are composed of interconnected feedback loops by sharing a set of molecular mechanisms that regulate the life activities of the human body at the transcriptional and translational levels. The forward loop includes Clock and Arntl (also called Bmal1 ), which are E-box transcription factors. Together, they induce transcription of the negative feedback regulators, Period (Per) and Cryptochrome (Cry) gene components. Clock mutant mice exhibit hypertriglyceridemia, likely due to overabsorption in the gut and oversynthesis in the liver. In Bmal1 knockout animals, mice exhibited short-term changes in lifespan, increased sleep duration, and altered blood pressure regulation, glucose homeostasis, lipid metabolism, and lipogenesis.

The human gut microbiota plays a very important role in maintaining homeostasis in the host individual. Its mechanisms include: through their metabolism, the host can not digest food into available nutrients, regulating energy metabolism; through their digestion of fiber food and decomposed metabolites, play a huge role in the development and maturation of the host immune system; Through the metabolites of the intestinal flora and the immune system, the maturation of intestinal morphology and function is jointly affected, preventing the invasion of pathogenic microorganisms and protecting the host from infection. The influence of gut microbiota on the physiological structure and function of the host is multifaceted, and there are many aspects that are poorly understood. The gut microbiota is complexly involved in the development of obesity-related metabolic diseases, such as nonalcoholic fatty liver disease (NAFLD), type 2 diabetes, and insulin resistance (IR), thus obesity underlies most metabolic diseases. Dietary obesity, as well as pre-type 2 diabetes, is characterized by altered gut microbiota, adipocyte attachment and disruption of the gut barrier. During obesity, the gut barrier is disrupted, gut tight junction protein expression is reduced, gut tightness is reduced, gut environment changes, and microbial composition and host interactions affect gut barrier function. Akkermansia is currently the only intestinal microbe that can be cultured in vitro in the genus Verrucobacterium. It was first isolated in 2004. As one of the most abundant bacterial species in the human intestinal tract, it accounts for 0.5%-5 %, it can break down mucus in the gut as a source of nutrition, and it is also one of the human gut microbes that can be more easily detected by metagenomic analysis, and the presence of this bacteria is related to rodent and human body weight. negatively correlated. By measuring the gut microbes of different populations, it can be found that the abundance of this bacteria in the intestines of patients with type 2 diabetes is significantly reduced; animal experiments have also proved that Akkermansia can reduce the weight gain of mice on a high-fat diet, and can also repair high-fat diets The resulting impaired epithelial integrity neutralizes endotoxin in mice and improves insulin resistance. Intestinal dysbiosis and circadian rhythm disturbances are associated with many diseases, including obesity, metabolic syndrome and inflammatory bowel disease. "Pre-diabetes" can be turned into a normal blood sugar state. Its characteristic is impaired glucose regulation. Obesity, damp-heat and phlegm-dampness constitution are the main types of constitutions with impaired glucose regulation. Oral hypoglycemic drugs such as sulfonylurea insulin secretagogues have a long cycle in the treatment of type 2 diabetes. Long-term oral administration not only has obvious side effects, but also gradually increases drug resistance and significantly deteriorates the prognosis.

Gegen Qinlian Decoction first appeared in "Treatise on Febrile Diseases". It is mainly used to treat dysentery and diarrhea caused by damp heat. It has also been reported in the literature that it can improve metabolic syndrome, lower blood sugar and lipid levels, and has anti-inflammatory and intestinal flora effects. For example: Li Yingmeng, Fan Xuemei, Wang Yiming, et al. Research progress of Gegen Qinlian Decoction in the treatment of type 2 diabetes [J]. Jiangxi Traditional Chinese Medicine, 2013, 44(11): 75-77; another example: Zeng Yipeng, Feng Xin Ge, Gu Chengying, et al. Effect of Gegen Qinlian Decoction on the intestinal flora of type 2 diabetes with damp-heat syndrome [J]. Hebei Medicine, 2016,22(10):1731-1734.; also such as: Feng Xinge, Yan Yuzhong, Zeng Yipeng, et al. Effects of Gegen Qinlian Decoction on intestinal flora of type 2 diabetes with damp-heat syndrome [J]. World Journal of Integrative Medicine, 2016,11(08):1110-1112.

But so far, there is no standard research on the biological rhythm of Gegen Qinlian Decoction water extract on the intestinal flora, blood sugar, blood lipid and insulin, and there is no rational use of traditional Chinese medicine four diagnosis and ginseng technology to investigate Gegenqin Pharmacological action method of Liantang water extract.

SUMMARY OF THE INVENTION

The technical problem to be solved by the present invention is: in view of the existing water extracts of Gegen Qinlian Decoction to regulate the expression levels of intestinal flora and liver biological clock genes in pre-diabetic obesity, and then to study and treat pre-diabetic obesity, there is still a lack of relevant reports. In view of the defects and deficiencies, the present technology provides a Gegen Qinlian decoction water extract and its application for regulating the intestinal flora of the obese body's intestinal dampness and heat and regulating the expression level of the liver biological clock gene. Based on the traditional TCM basic theory of "same treatment for different diseases", the present invention combines the new progress of modern disease mechanism research, and explores its treatment of glucose and lipid metabolism disorders from the perspectives of the regulation of intestinal flora and the expression level of liver biological rhythm genes. Gegen Qinlian Decoction water extract can effectively regulate the expression level of intestinal flora and liver biological clock gene expression level of intestinal dampness and heat in pre-diabetic obese body, reduce the body's serum triglyceride level, and achieve the purpose of effectively treating pre-diabetic obesity and preventing type 2 diabetes. .

The present invention adopts the following technical solutions to achieve the purpose of the invention.

First of all, the present invention discloses a Gegen Qinlian Decoction water extract for regulating the expression level of intestinal flora and liver biological clock gene in the intestinal damp heat of obese body.

The Gegen Qinlian Decoction water extract is obtained from Pueraria lobata, Scutellaria baicalensis, Rhizoma Coptidis, and Zhigancao through the compatibility of medicinal materials and water decoction.

Further, the medicinal material compatibility is calculated as: 7-9 parts of Pueraria lobata, 2-4 parts of Scutellaria baicalensis, 2-4 parts of Coptis chinensis, 1-3 parts of Zhigancao; preferably, 8 parts of Pueraria lobata, 3 parts of Scutellaria baicalensis , 3 parts of Coptis chinensis, 2 parts of Zhigancao.

Further, the water decocting includes the steps of soaking in water, heating and decocting, and concentrating under reduced pressure.

Further, the described soaking in water is as follows: adding 5-10 parts by weight of pure water to 1 part by weight of the medicinal material, and soaking in water for 30-60 min; Heating to boiling, maintaining the decoction for 30-60min and filtering to obtain the decoction filtrate, repeating the decoction, and combining the decoction filtrate; the described decompression concentration is: the combined decoction filtrate is under the vacuum condition of ≤-0.08MPa, The crude medicinal material is concentrated to 1 weight part at low temperature to obtain 1 volume part of extract concentrate, that is, 1 gram of crude medicinal material is soaked-decocted-concentrated to obtain 1 ml of concentrated liquid, and the concentration temperature is 60°C-65°C.

Further, the Gegen Qinlian Decoction water extract is excipient to prepare a preparation; the preparation is selected from one of granules, pills, capsules, tablets, powders, ointments and oral liquids.

Secondly, the present invention discloses a use of the above-mentioned Gegen Qinlian Decoction water extract.

The Gegen Qinlian Decoction water extract is used for preparing medicines for regulating the intestinal flora and liver biological clock genes of the intestinal dampness and heat of the obese body. The Gegen Qinlian Decoction water extract has the effect of reversing Akkermansia and Lactobacillus bacteria, at the same time up-regulating the expression and transcription levels of the liver Clock and Arntl genes, and can effectively reduce the serum triglyceride level of the body. Protein cholesterol, blood sugar, insulin and other blood lipids and blood sugar indicators have regulatory effects, as well as body metabolites such as short-chain, medium-chain and long-chain fatty acids, tryptophan, kynuric acid, and cannabinoids.

Further, the obese body is an obese body with intestinal flora imbalance caused by obesity, or a biological rhythm disorder caused by intestinal flora disorder and liver biological clock gene changes, and then obesity or high-fat obesity with metabolic syndrome. obesity or pre-type 2 diabetes mellitus.

The intestinal flora is selected from one or more of norank_f__Bacteroidales_S24-7_group , Bacteroides , Lachnospiraceae_NK4A136_group , unclassified_f__Lachnospiraceae , Clostridium_sensu_stricto_1 , and Akkermansia .

The liver biological clock gene is selected from one or more of Arnt1 , Clock, Per3 and Scd1 mRNA transcription and expression levels.

Further, the intestinal flora is preferably Akkermansia and/or Lactobacillus ; the liver biological clock gene is preferably Arnt1 and/or Clock gene.

Beneficial effects:

(1) The present invention is based on the traditional TCM basic theory of "same treatment for different diseases", combined with the new progress of modern disease mechanism research, and explores its treatment of glucose and lipid metabolism disorders, prevention and treatment of prediabetes from the perspective of the regulation of intestinal flora and biological rhythms The Gegen Qinlian Decoction water extract obtained by the present invention can effectively regulate the intestinal flora and the expression level of liver biological clock genes in the intestinal dampness and heat of the obese body with pre-diabetes, and can effectively prevent and treat obesity and insulin resistance in the pre-diabetic body. In the late stage of obesity, obesity manifests as Akkermansia (Akk) and liver Clock and Arntl genes up-regulated at the transcriptional level, while Lactobacillus (Lactobacillus) is down-regulated. This significant change in biological rhythms and flora will be a sensitive signal. For the obesity stage of pre-diabetes, it can be used as a marker for clinical detection, which is of great significance in the prevention and treatment of diabetes.

(2) The present invention is based on the weight gain of obese rats caused by high-fat diet, which will cause changes in blood biochemical indicators (including increased triglyceride levels), changes in the composition and structure of intestinal flora, and cause biorhythm disorders (shown here). The effect of liver biological clock gene), that is, high-fat diet will change intestinal flora metabolism and liver biological rhythm, convert over-digested food into usable nutrients, change energy metabolism, reduce lipid metabolism, make Fat accumulates in the body, resulting in elevated triglycerides in the body's blood. The Gegen Qinlian Tang water extract obtained by the invention has the effect of reversing the up-regulation of intestinal flora Akkermansia (Akk bacteria), Lactobacillus (Lactobacillus (Lactobacillus) and liver Arntl and Clock genes, and can effectively reduce the serum triglyceride level of the body.

Description of drawings

Figure 1 shows the changes in body weight, body length and Lee's index of rats during modeling.

Figure 2 shows the changes of blood lipid indexes in the 4th week of high-fat diet-induced obesity rat model.

Figure 3 shows the changes in blood lipids before and after GQD administration.

Figure 4 shows the abundance changes of Akk (Akkermansia) before and after GQD administration;

Among them: C means normal group, OB means model group, LD means Gegen Qinlian Decoction low dose group, MD means Gegen Qinlian Decoction medium dose group, HD means Gegen Qinlian Decoction high dose group, SIM means Simvastatin group.

Figure 5 shows the abundance changes of Lactobacillus before and after GQD administration;

Among them: C means normal group, OB means model group, LD means Gegen Qinlian Decoction low dose group, MD means Gegen Qinlian Decoction medium dose group, HD means Gegen Qinlian Decoction high dose group, SIM means Simvastatin group.

Figure 6 shows the changes of Arntl gene before and after GQD administration.

Figure 7 shows the changes of Clock gene before and after GQD administration.

Detailed ways

The present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited to the following embodiments. The methods are conventional methods unless otherwise specified. The raw materials can be obtained from open commercial sources unless otherwise specified.

Example 1: Preparation of Gegen Qinlian Decoction Water Extract Oral Liquid

Compatibility of medicinal materials: 8 parts of Pueraria lobata, 3 parts of Scutellaria baicalensis, 3 parts of Coptis chinensis, and 2 parts of Zhigancao in parts by mass.

Soaking in water: Add 1 kg of the above weighed and compatible medicinal materials, add 8 kg of clean water at a water ratio of 1:8, and soak in water for 30-60 minutes;

Heating and decocting: heat the above soaked medicinal materials and soaking water to boiling, maintain the decocting for 30-60min and filter to obtain the decoction filtrate, repeat the decoction once, and combine the decoction filtrate;

Concentration under reduced pressure: the above-mentioned combined decoction filtrate is concentrated at a low temperature under vacuum conditions of ≤-0.08MPa until the volume of the concentrated solution is only 1 liter, and the concentration temperature is 60°C-65°C.

The preparation is prepared; the concentrated solution of the Gegen Qinlian Decoction water extract obtained by concentrating under reduced pressure is sterilized and subpackaged to prepare an oral liquid.

Example 2: Animal test for regulating intestinal flora in obese body

1. Experimental scheme

A total of 90 rats in this batch were randomly divided into blank group and obesity model group. The blank group was fed with normal feed containing 10% fat, and the model group was fed with high-fat feed containing 60% fat. , followed the modeling control group (Control, referred to as C), given ordinary feed. The model group was randomly divided into obesity model group (OB), GQD high-dose group (HD), GQD medium-dose group (MD), GQD low-dose group (LD), and simvastatin group (SIM) according to body weight. only, given a high-fat diet. The dose of the drug in the administration group was calculated per kilogram of rat body weight, and was administered by gavage once a day until the 17th week. The specific administration of animals in each group is as follows, and the following is the dose per kilogram of rat body weight:

Simvastatin group (SIM): 4mg/kg

GQD high-dose group (HD): 14.85ml/kg of the oral liquid prepared in Example 1

GQD middle dose group (MD): the oral liquid prepared in Example 1 4.85ml/kg

GQD low-dose group (LD): 1.65ml/kg of the oral liquid prepared in Example 1

2. Detection plan

(1) Blood sugar and blood lipid testing:

All serum samples were sent to Jiangxi Provincial Hospital of Traditional Chinese Medicine for determination of blood lipid components (GLU, LDL-C, HDL-C, TC, TG and insulin). The detection instrument is an automatic biochemical analyzer: Roche Modular P800 (Roche).

(2) Gut microbial DNA extraction and 16S rRNA sequencing:

The total DNA of intestinal microorganisms was extracted by Qiagen DNA Mini Kit method, the concentration of which was measured by the nucleic acid protein analyzer NanoDrop ND-2000, and the integrity of DNA molecules was detected by agarose. The sequencing library was purified, quantified and homogenized, and high-throughput sequencing was performed on the Illumina HiSeq 2500 platform by paired-end sequencing (Shanghai Meiji Biomedical Technology Co., Ltd.).

(3) q-PCR (TaqMan) method to detect the mRNA transcription and expression levels of clock genes Clock and Arntl in rat liver:

Total RNA from liver tissue was extracted by Trizol method, and the target gene was quantitatively detected by TaqMan probe hydrolysis method using LightCycler® 480 II high-throughput fluorescence quantitative PCR instrument. The fluorophore is attached to the 5' end of the probe, which is called the reporter group (Reporter, R). In this study, FAM was used, and the 3' end was attached to the quencher group (Quencher, Q).

3. Test results

(1) Observation of urine and feces

Compared with the blank group, the SD rats in the three high-fat diet-fed model groups significantly reduced their activity and diet, gained weight, experienced a dull-to-yellow coat color, severe hair loss, tooth marks, and white, thick and greasy fur. Also had loose stools, yellow urine and feces.

(2) There were significant differences in weight, body length and Lee's index in Western medicine clinical tests ( P < 0.01, see Figure 1).

(3) The biochemical indicators of blood lipids were detected in 4 weeks, and it was found that there were significant differences in cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HLD) between the two models ( P ＜ 0.01). ). Changes in physical signs and biochemical indicators have the same trend for the success of obesity model modeling (see Figure 2).

(4) After the successful modeling in the 4th week, the obesity model group was randomly divided into groups, and the drug was administered at the end of the 5th week, and the serum TG content was measured at the 10th, 12th, and 16th weeks. Compared with the SIM group, it was found that GQD had a better effect on reducing TG, and the serum biochemical indicators tended to be in the blank group, and the levels of TG were shown in Table 1-7 and Figure 1-3. image 3).

(5) At the phylum level of intestinal flora in the model group and the blank group: the types of flora are the same, but the proportions are different. In the blank group, Bacteroidetes was the highest (56.32%), Firmicutes was the second (40.32%), Verrucobacterium (1.99%) was the second. In the model group, Firmicutes was the highest (57.00%), followed by Bacteroidetes (33.84%) and Verrucobacterium (7.06%) (see the table below).

(6) In the blank group:

norank_f__Bacteroidales_S24-7_group is the highest (38.59%),

Bacteroides followed (17.03%),

Lachnospiraceae_NK4A136_group (10.31%) followed,

unclassified_f__Lachnospiraceae (4.02%);

In the obesity model group:

norank_f__Bacteroidales_S24-7_group is the highest (23.83%),

Bacteroides followed (9.26%),

Clostridium_sensu_stricto_1 followed (7.65%).

Among them, the abundance of Akkermansia bacteria in the model group was higher than that in the blank group (this result is different from previous literature reports, see the table below).

(7) norank_f__Bacteroidales_S24-7_group, Bacteroides, Lachnospiraceae_NK4A136_group , unclassified_f__Lachnospiraceae, norank_f__Clostridiales_vadinBB60_group were highly abundant in the blank group, with significant differences;

Akkermansia , Romboutsia, Clostridium_sensu_stricto_1, Blautia, Epulopiscium, Anaerotruncus, norank_f__Lachnospiraceae, norank_f__Peptococcaceae obesity model group were high, with significant differences.

Among them, Akkermansia bacteria in previous studies generally showed a lower abundance of Akkermansia bacteria in obese individuals, which was negatively correlated with type 2 diabetes. (2018-07-31, Article, 10.1136/gutjnl-2017-315565; 2018-02-10, Comment, 10.1136/gutjnl-2017-315732), on the contrary in this test result, Akkermansia bacteria in obese rats higher abundance.

At the same time, some bacterial groups consistent with previous studies were also found. For example, the abundance of Bacteroides was higher in the blank (2006-12-21, Article, 10.1038/nature05414) group, and Blautia and Anaerotruncus were higher in the obesity model group (J Ginseng Res. 2014 Apr;38(2):106- 15.doi: 10.1016/j.jgr.2013.12.004. Epub 2014 Jan 9.) (see table below).

(8) Bacteroides, Akkermansia, Marvinbryantia, Subdoligranulum, norank_f__Peptococcaceae , unclassified_f__Lachnospiraceae, norank_f__Ruminococcaceae, Ruminococcaceae_UCG-005 increased significantly after treatment;

norank_f__Bacteroidales_S24-7_group, Blautia, Coprococcus_1, Lachnospiraceae_NK4A136_group , Clostridium_sensu_stricto_1, unclassified_o__Bacteroidales decreased after treatment.

Where Blautia is associated with obesity, Akkermansia is generally enriched in healthy individuals and reduced in obese and diabetic patients.

(9) Bacteroides, Akkermansia, Marvinbryantia, Subdoligranulum, norank_f__Peptococcaceae , unclassified_f__Lachnospiraceae, norank_f__Ruminococcaceae, Ruminococcaceae_UCG-005 increased significantly after treatment;

norank_f__Bacteroidales_S24-7_group, Blautia, Coprococcus_1, Lachnospiraceae_NK4A136_group , Clostridium_sensu_stricto_1, unclassified_o__Bacteroidales decreased after treatment. Where Blautia is associated with obesity, Akkermansia is generally enriched in healthy individuals and reduced in obese and diabetic patients. The microbiota changes were consistent with high-dose treatment.

（10） Bacteroides、Ruminococcaceae_UCG-005、norank_f__Ruminococcaceae、 Parabacteroides、Akkermansia、Christensenellaceae_R-7_group在 治疗后增加，而norank_f__Bacteroidales_S24-7_group、Romboutsia、Lachnospiraceae_NK4A136_group、 Coprococcus_1、Anaerotruncus、Oscillibacter、Epulopiscium、Tyzzerella、 Erysipelatoclostridium治疗后减少。

Where Blautia is associated with obesity, Akkermansia is generally enriched in healthy people and reduced in obese and diabetic patients, while the results of this study are just the opposite. Akkermansia generally grows on the intestinal wall and plays a role in the intestinal barrier function. The results of this study increased significantly in the model group, which may be related to the damage of the intestinal wall and a large amount of feces falling into the intestinal tract. Further, the expression levels of the intestinal tight junction proteins Claudin1 and Zo1 were analyzed. Compared with the blank group, the expression levels of the model group showed a significant downward trend (P<0.01, see the table below).

The relative gray value comparison of the two proteins ( ±s) In addition, the changes before and after administration of Akkermansia and Lactobacillus were also analyzed, and it was found that low-dose Gegen Qinlian Decoction significantly down-regulated Akkermansia , while high-dose Gegen Qinlian Decoction significantly increased Lactobacillus (see Figure 4 and Figure 5).

Figure 4 shows the changes in the abundance of Akk (Akkermansia) genus before and after GQD administration. Among them: C means normal group, OB means model group, LD means Gegen Qinlian Decoction low dose group, MD means Gegen Qinlian Decoction medium dose group, HD means Gegen Qinlian Decoction high dose group, SIM means Simvastatin group.

Figure 5 shows the changes of Lactobacillus abundance before and after GQD administration. Among them: C means normal group, OB means model group, LD means Gegen Qinlian Decoction low dose group, MD means Gegen Qinlian Decoction medium dose group, HD means Gegen Qinlian Decoction high dose group, SIM means Simvastatin group. .

(11) In SIM group and OB group, there are only Bacteroides (enriched in OB group) and Lachnospiraceae_NK4A136_group (enriched in OB group).

(12) It can be observed from the results of liver q-PCR (Figure 6 shows the changes of Arntl gene before and after GQD administration; Figure 7 shows the changes of Clock gene before and after GQD administration), the expression of Clock and Arntl genes in the obesity model group is higher than that in the blank group. The level of mRNA expression was up-regulated, and the obesity model group had a significant difference in Arntl gene compared with the blank group (P<0.05). After administration, the mRNA expression level of Clock gene GQD in the middle-dose and low-dose groups was significantly different from that in the model group (P<0.05). 0.05), and up-regulated the expression of this gene; the mRNA expression levels of Arntl gene simvastatin group and GQD administration groups at different concentrations were significantly different from those in the model group (P<0.05), showing an up-regulated trend. It indicated that Gegen Qinlian Decoction could up-regulate the transcriptional expression levels of the hepatic biological clock genes Clock and Arntl genes.

The specific embodiments of the present invention have been described above in detail, but they are only used as examples, and the present invention is not limited to the specific embodiments described above. For those skilled in the art, any equivalent modifications and substitutions to the present invention are also within the scope of the present invention. Therefore, equivalent changes and modifications made without departing from the spirit and scope of the present invention are all included within the scope of the present invention.

Claims (3)

1. A Gegen Qinlian Tang water extract for regulating an obese body, is characterized in that: obtained through medicinal material compatibility and water decocting by Pueraria lobata, Scutellaria baicalensis, Coptis chinensis, Zhigancao; The said medicinal material compatibility is calculated as: 7-9 parts of Pueraria lobata, 2-4 parts of Scutellaria baicalensis, 2-4 parts of Coptis chinensis, and 1-3 parts of Zhigancao; Described decoction in water: including the steps of soaking in water, heating and decoction, and decompression concentration; The described soaking in water is as follows: adding 5-10 parts by weight of pure water according to the ratio of 1 part by weight of medicinal materials, and soaking in water for 30-60min; The heating decoction is as follows: heating the soaked medicinal materials and soaking water to boiling, maintaining the decoction for 30-60min and filtering to obtain a decoction filtrate, repeating the decoction, and combining the decoction filtrate; The described concentration under reduced pressure is as follows: under the vacuum condition of ≤-0.08MPa, the combined decoction filtrate is concentrated to 1 weight part of the original medicinal material at low temperature to obtain 1 volume part of the extract concentrate, and the concentration temperature is 60 ℃-65 ℃. °C. 2. a kind of Gegen Qinlian Decoction water extract for regulating obese body according to claim 1, is characterized in that, described Gegen Qinlian Decoction water extract, excipient is made into preparation; Described preparation is selected from One of granules, pills, capsules, tablets, powders, ointments, and oral liquids. 3. A use of the water extract of Gegen Qinlian Decoction according to any one of claims 1-2, characterized in that it is used to prepare medicines for regulating intestinal flora and liver biological clock genes of obese body's intestinal damp-heat.