CN110464350B - Microwave microscopic imaging method, device and system - Google Patents
Microwave microscopic imaging method, device and system Download PDFInfo
- Publication number
- CN110464350B CN110464350B CN201910774539.5A CN201910774539A CN110464350B CN 110464350 B CN110464350 B CN 110464350B CN 201910774539 A CN201910774539 A CN 201910774539A CN 110464350 B CN110464350 B CN 110464350B
- Authority
- CN
- China
- Prior art keywords
- microwave
- photoacoustic signal
- absorption coefficient
- excitation point
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/05—Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; Measuring using microwaves or radio waves
- A61B5/0507—Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; Measuring using microwaves or radio waves using microwaves or terahertz waves
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/1702—Systems in which incident light is modified in accordance with the properties of the material investigated with opto-acoustic detection, e.g. for gases or analysing solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N22/00—Investigating or analysing materials by the use of microwaves or radio waves, i.e. electromagnetic waves with a wavelength of one millimetre or more
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Radiology & Medical Imaging (AREA)
- Medical Informatics (AREA)
- Electromagnetism (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
Abstract
本发明提供了一种微波显微成像方法、装置及系统，涉及生物医学成像技术领域。该方法通过获取对待测样品包含的多个激发点中的目标激发点使用脉冲微波加热前、后的第一光声信号及第二光声信号，然后根据第一光声信号及第二光声信号确定目标激发点的微波吸收系数，从而根据每个激发点的位置坐标及对应的微波吸收系数得到待测样品的微波显微图像。由于第二光声信号和第一光声信号两者的差值包括脉冲微波导致的目标激发点的温度变化情况，从而可以根据第二光声信号和第一光声信号两者确定微波吸收系数；又由于激光可以聚焦，从而减小照射至激发点的光斑的面积，使得待测样品可以测定的激发点更多，进而提高了图像分辨率，实现了微波显微成像。
The invention provides a microwave microscopic imaging method, device and system, and relates to the technical field of biomedical imaging. The method obtains the first photoacoustic signal and the second photoacoustic signal before and after the target excitation point among the multiple excitation points contained in the sample to be tested is heated by pulsed microwaves, and then according to the first photoacoustic signal and the second photoacoustic signal The signal determines the microwave absorption coefficient of the target excitation point, so that the microwave microscopic image of the sample to be tested is obtained according to the position coordinates of each excitation point and the corresponding microwave absorption coefficient. Since the difference between the second photoacoustic signal and the first photoacoustic signal includes the temperature change of the target excitation point caused by the pulsed microwave, the microwave absorption coefficient can be determined according to both the second photoacoustic signal and the first photoacoustic signal ; and because the laser can be focused, thereby reducing the area of the light spot irradiated to the excitation point, so that more excitation points can be measured for the sample to be tested, thereby improving the image resolution and realizing microwave microscopic imaging.
Description
Technical Field
The invention relates to the technical field of biomedical imaging, in particular to a microwave microscopic imaging method, a device and a system.
Background
The microwave imaging uses the electromagnetic wave of microwave frequency spectrum as a detection means to obtain the spatial distribution of dielectric properties in a living body, diagnoses the pathological change tissue by presenting the difference of the dielectric properties among different tissues, the method mainly comprises the steps of detecting the electromagnetic wave of a sample to be detected implanted into a coupling medium, realizing the omnibearing collection and monitoring of scattered waves at a receiving end by rotating an antenna array, and finally obtaining the dielectric property distribution of the sample to be detected by utilizing a repeated iterative algorithm.
Microwave thermoacoustic imaging is a new nondestructive medical imaging method developed in recent years, and is a tomography technology for exciting thermoacoustic signals (ultrasonic signals) by irradiating biological tissues with pulse microwaves. Therefore, thermoacoustic imaging is a lossless, high contrast, deep imaging depth, large field of view imaging method.
However, since microwaves are difficult to focus, microwave imaging resolution depends on microwave wavelength (in the order of centimeters), and microwave thermoacoustic imaging improves resolution to acoustic resolution (in the order of sub-millimeters), general microwave imaging resolution is not high.
Disclosure of Invention
In view of the above, the present invention provides a method, an apparatus and a system for microwave microscopic imaging.
In order to achieve the above purpose, the embodiment of the present invention adopts the following technical solutions:
in a first aspect, an embodiment of the present invention provides a microwave microscopy imaging method applied to a processor of a microwave microscopy imaging system, where the method includes:
acquiring a first photoacoustic signal and a second photoacoustic signal, wherein the first photoacoustic signal and the second photoacoustic signal are respectively photoacoustic signals before and after heating a target excitation point in a plurality of excitation points included in a sample to be measured by using pulse microwaves;
determining a microwave absorption coefficient of the target excitation point according to the first photoacoustic signal and the second photoacoustic signal, wherein the microwave absorption coefficient corresponds to the excitation point;
and obtaining a microwave microscopic image of the sample to be detected according to the position coordinate of each excitation point and the corresponding microwave absorption coefficient.
In a second aspect, an embodiment of the present invention provides a microwave microscopy imaging apparatus applied to a processor of a microwave microscopy imaging system, where the apparatus includes:
the photoacoustic signal acquisition module is used for acquiring a first photoacoustic signal and a second photoacoustic signal, wherein the first photoacoustic signal and the second photoacoustic signal are respectively photoacoustic signals before and after heating a target excitation point in a plurality of excitation points contained in a sample to be measured by using pulse microwaves;
a microwave absorption coefficient determining module, configured to determine a microwave absorption coefficient of the target excitation point according to the first photoacoustic signal and the second photoacoustic signal, where the microwave absorption coefficient corresponds to the excitation point;
and the image generation module is used for obtaining a microwave microscopic image of the sample to be detected according to the position coordinate of each excitation point and the corresponding microwave absorption coefficient.
In a third aspect, embodiments of the present invention provide a microwave microscopy imaging system comprising a processor configured to perform the microwave microscopy imaging method according to any one of the preceding embodiments.
According to the microwave microscopic imaging method, device and system provided by the embodiment of the invention, the first photoacoustic signal and the second photoacoustic signal before and after the target excitation point in the multiple excitation points included in the sample to be detected is heated by using the pulse microwaves are obtained, and then the microwave absorption coefficient of the target excitation point is determined according to the first photoacoustic signal and the second photoacoustic signal, so that the microwave microscopic image of the sample to be detected is obtained according to the position coordinate of each excitation point and the corresponding microwave absorption coefficient. Since the difference value of the second photoacoustic signal and the first photoacoustic signal includes the temperature change of the target excitation point caused by the pulse microwave, the microwave absorption coefficient can be determined according to the second photoacoustic signal and the first photoacoustic signal; and because the laser can be focused, the area of a light spot irradiated to an excitation point is reduced, so that more excitation points can be measured by the sample to be measured, the image resolution is improved, and the submicron-order microwave microscopic imaging is realized.
In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 shows a structural block diagram of a microwave microscopic imaging system provided by the invention.
Fig. 2 shows a flow chart of a microwave microscopic imaging method provided by the invention.
Fig. 3 shows a further flow chart of the microwave microscopic imaging method provided by the invention.
Fig. 4 shows a schematic diagram of a microwave micro-imaging method provided by the invention.
Fig. 5 shows a functional block diagram of a microwave micro-imaging device provided by the invention.
Icon: 100-a microwave microscopic imaging system; 110-a processor; 120-a laser generating unit; 130-a microwave generating unit; 140-an ultrasound detection unit; 150-a coupling unit; 160-a data acquisition unit; 200-a microwave microscopic imaging device; 210-a photoacoustic signal acquisition module; 220-microwave absorption coefficient determining module; 230-image generation module.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
It is noted that relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising a … …" does not exclude the presence of another identical element in a process, method, article, or apparatus that comprises the element.
The invention provides a microwave microscopic imaging system 100 for obtaining a microwave microscopic image with high resolution. Fig. 1 is a block diagram of a microwave microscopy imaging system 100 according to the present invention. The microwave microscopic imaging system 100 provided by the invention comprises: a processor 110, a laser generating unit 120, a microwave generating unit 130, an ultrasonic detecting unit 140, a coupling unit 150, a data acquiring unit 160, and a focusing unit (not shown). The processor 110 is electrically connected to the laser generating unit 120, the microwave generating unit 130 and the data collecting unit 160, and the ultrasonic detecting unit 140, the data collecting unit 160 and the processor 110 are electrically connected in sequence.
The laser generating unit 120 is configured to emit pulsed laser to a target excitation point of a sample to be measured disposed in the coupling unit 150 under the control of the processor 110. In an alternative embodiment, the laser generating unit 120 emits pulsed laser light with a pulse width of 10ns, a pulse repetition frequency of 10Hz, and a wavelength of 1206mm.
The focusing unit is disposed between the laser generating unit 120 and the coupling unit 150, and is configured to focus the pulse laser emitted by the laser generating unit 120 to reduce the area of the light spot.
The microwave generating unit 130 is used for emitting pulsed microwaves to a target excitation point of a sample to be measured, at a controller of the processor 110. In an alternative embodiment, the microwave generating unit 130 is a BW-6000HPT high power microwave generator with a frequency of 6GHz and a continuously adjustable pulse power in the range of 80KW-300 KW. The pulse width of the pulse microwave is 0.5 mu s, and the repetition frequency is 50-500 Hz, preferably 500Hz.
The coupling unit 150 is used for placing a sample to be measured. Specifically, the coupling unit 150 may include a light-transmitting and microwave-transmitting container and a coupling agent in the container, and the sample to be tested is placed in the coupling agent. The couplant enables the sample to be detected to be in a constant temperature environment, and meanwhile, the photoacoustic signals can be effectively transmitted to the ultrasonic detection unit 140, so that imaging is clearer.
The ultrasonic detection unit 140 is configured to receive the ultrasonic signal, convert the ultrasonic signal into an electrical signal, and transmit the electrical signal to the processor 110. In an alternative embodiment, the ultrasonic detection unit 140 is a multi-element linear array probe, the main frequency is 10MHz, and the relative bandwidth is about 70%.
The data acquisition unit 160 is configured to perform frequency selection, filtering, analog-to-digital conversion and other operations on the electrical signal transmitted by the ultrasound detection unit 140 to obtain a photoacoustic signal, and transmit the photoacoustic signal to the processor 110.
The processor 110 is configured to determine a microwave absorption coefficient of each excitation point, and perform image reconstruction using a matlab program to obtain a microwave microscopic image and perform image display.
The invention also provides a microwave microscopic imaging method, which is applied to the processor 110 of the microwave microscopic imaging system 100 and used for obtaining a microwave microscopic image with high resolution. Fig. 2 is a flowchart of a microwave micro-imaging method according to the present invention. The microwave microscopic imaging method comprises the following steps:
s201, a first photoacoustic signal and a second photoacoustic signal are obtained.
The first photoacoustic signal and the second photoacoustic signal are photoacoustic signals before and after heating a target excitation point in a plurality of excitation points included in a sample to be measured by using pulse microwaves.
Fig. 3 is a flowchart of a microwave microscopy imaging method. The S201 includes:
s2011, the laser generating unit 120 is controlled to emit the pulsed laser to the target excitation point.
First, the processor 110 controls the laser generating unit 120 to emit pulsed laser with specific parameters, and the pulsed laser is focused by the focusing unit and then radiated to the target excitation point.
S2012, the first photoacoustic signal collected by the ultrasound detection unit 140 is received.
The ultrasound detection unit 140 acquires a first photoacoustic signal and transmits the first photoacoustic signal to the processor 110. Since the microwave generating unit 130 is not yet operating, the collected first photoacoustic signal is the photoacoustic signal before the target excitation point is heated by using the pulsed microwave.
S2013, after the first preset time, controlling the microwave generating unit 130 to emit the pulsed microwave to the target excitation point for a preset time to heat the target excitation point.
That is, after the sample to be measured is cooled for the first preset time, the microwave generating unit 130 is controlled to emit the pulse microwaves with specific parameters to the target excitation point, so that the target excitation point is heated. If the first photoacoustic signal is acquired to directly control the microwave generating unit 130 to emit the pulse microwave to the target excitation point and continue for a preset time, the temperature variation of the target excitation point is not completely determined by the pulse microwave due to the existence of heat accumulation, so that the heat accumulation can be avoided by cooling the sample to be tested for the first preset time, and an accurate test result can be obtained.
Typically, the first predetermined time is 60 to 100s. Preferably, the first preset time is 100s.
In addition, in order to enable the sample to be measured to rise to a sufficient temperature under the irradiation of the pulsed microwave, the pulsed microwave needs to be continuously emitted to the target excitation point for a preset time. In an alternative embodiment, the preset time period is 10 to 60s, and preferably, the preset time period is 20s.
S2014, the laser generating unit 120 is controlled again to emit a pulse laser to the target excitation point and receive the second photoacoustic signal collected by the ultrasound detecting unit 140.
It is understood that the second photoacoustic signal is a photoacoustic signal after heating the target excitation point by using pulsed microwaves.
In addition, S2014 is consistent with S2011, which is not described herein again. It should be noted, however, that the time interval between the two steps S2014 and S2013 should be as small as possible to avoid thermal diffusion, resulting in inaccurate final measurement results.
S202, determining the microwave absorption coefficient of the target excitation point according to the first photoacoustic signal and the second photoacoustic signal.
Wherein the microwave absorption coefficient corresponds to the excitation point. That is, each excitation point has a corresponding microwave absorption coefficient.
Please refer to fig. 3, which is a flowchart illustrating the process of S202. The S202 includes:
s2021, determining a temperature variation of the target excitation point according to the first photoacoustic signal and the second photoacoustic signal.
Specifically, the first photoacoustic signal, the second photoacoustic signal, and the temperature variation satisfy the following equations:
wherein, PA 0 Is a first photoacoustic signal, and PA 0 ∝αΓ＝α(AT 0 + B) (i.e. PA) 0 And Alpha (AT) 0 + B) proportional relationship), PA 1 Is a second photoacoustic signal, and PA 1 ∝α[A(T 0 +ΔT)+B](i.e., PA) 1 And alpha [ A (T) 0 +ΔT)+B]Proportional relation), alpha is a preset laser absorption coefficient, A is a preset first coefficient, B is a preset second coefficient, T 0 Δ T is a temperature change amount for the indoor ambient temperature acquired in advance.
S2022, determining the microwave absorption coefficient of the target excitation point based on the temperature variation and the preset parameter set.
The preset parameter set comprises preset duration of microwave radiation, microwave repetition frequency, microwave energy, density of the sample to be detected and specific heat capacity of the sample to be detected. Thus, the temperature variation, the preset parameter set and the microwave absorption coefficient satisfy the following formula:
wherein, delta T is temperature variation, T is preset duration of microwave radiation, f is microwave repetition frequency, beta is microwave absorption coefficient, H is microwave energy, rho is density of a sample to be measured, C p The specific heat capacity of the sample to be measured.
It should be noted that the preset duration of the microwave radiation is the preset duration, the microwave repetition frequency f and the microwave energy H are parameters of the pulse microwaves emitted by the microwave generating unit 130, and the density of the sample to be measured and the specific heat capacity of the sample to be measured are parameters known in advance.
The two equations are combined to obtain:
thus, microwave absorption coefficient
And S203, obtaining a microwave microscopic image of the sample to be detected according to the position coordinates of each excitation point and the corresponding microwave absorption coefficient.
In an alternative embodiment, the processor 110 may be utilized to run a matlab program to perform image reconstruction to obtain a microwave microscope image.
That is, after the microwave absorption coefficient of each excitation point is obtained, a microwave microscopic image of the sample to be measured can be obtained. The target excitation point is replaced by adjusting the relative position between the sample to be measured and the laser generating unit 120.
In an optional embodiment, the microwave microscopy imaging system further includes a sample position adjusting unit, and the sample position adjusting unit is electrically connected to the processor 110, the sample position adjusting unit is disposed in the coupling unit, and the sample to be measured is placed on the sample position adjusting unit. Therefore, each time the microwave absorption coefficient of a target excitation point is determined, the processor 110 controls the sample position adjusting unit to adjust the relative position between the sample phase to be measured and the laser generating unit 120, so as to replace the target excitation point, thereby determining the microwave absorption coefficient of a new target excitation point.
Fig. 4 is a schematic diagram of a microwave microscopy imaging method according to the present invention. After the sample to be detected is heated by using the pulse microwave, the difference value of the second photoacoustic signal and the first photoacoustic signal carries temperature rise information; and because the temperature rise information is related to the microwave absorption coefficient of the sample to be detected to the pulse microwave, the microwave absorption coefficient can be obtained under the condition that other parameters of the pulse microwave are determined. Because the laser can be focused, the area of a light spot irradiated to the excitation point is reduced, and the area occupied by each excitation point is smaller under the condition that the area of the sample to be detected is not changed, so that the number of the excitation points which can be measured on the sample to be detected is increased, the image resolution is improved, and the microwave microscopic imaging is realized.
In order to perform the corresponding steps in the above embodiments and various possible manners, an implementation manner of the microwave micro-imaging apparatus 200 is given below, and optionally, the microwave micro-imaging apparatus 200 may adopt the device structure of the processor 110 shown in fig. 1. Further, referring to fig. 5, fig. 5 is a functional block diagram of a microwave micro-imaging device 200 according to an embodiment of the present invention. It should be noted that the basic principle and the technical effects of the microwave microscopic imaging apparatus 200 provided in the present embodiment are the same as those of the above embodiments, and for the sake of brief description, no part of the present embodiment is mentioned, and reference may be made to the corresponding contents in the above embodiments. The microwave microscopic imaging apparatus 200 includes: a photoacoustic signal acquisition module 210, a microwave absorption coefficient determination module 220, and an image generation module 230.
The photoacoustic signal acquiring module 210 is configured to acquire a first photoacoustic signal and a second photoacoustic signal.
Specifically, the photoacoustic signal acquiring module 210 is configured to control the laser generating unit 120 to emit pulse laser to the target excitation point, then receive a first photoacoustic signal acquired by the ultrasonic detecting unit 140, then control the microwave generating unit 130 to emit pulse microwave to the target excitation point for a preset time after a first preset time, and finally control the laser generating unit 120 to emit pulse laser to the target excitation point again and receive a second photoacoustic signal acquired by the ultrasonic detecting unit 140.
It is to be understood that, in an alternative embodiment, the photoacoustic signal acquiring module 210 may be configured to perform steps S201, S2011, S2012, S2013 and S2014.
The microwave absorption coefficient determining module 220 is configured to determine a microwave absorption coefficient of the target excitation point according to the first photoacoustic signal and the second photoacoustic signal.
Specifically, the microwave absorption coefficient determining module 220 is configured to determine a temperature variation of the target excitation point according to the first photoacoustic signal and the second photoacoustic signal, and determine a microwave absorption coefficient of the target excitation point based on the temperature variation and a preset parameter set.
It is understood that in an alternative embodiment, the microwave absorption coefficient determining module 220 may be configured to perform S202, S2021, and S2022.
The image generating module 230 is configured to obtain a microwave microscopic image of the sample to be detected according to the position coordinate of each excitation point and the corresponding microwave absorption coefficient.
It is to be appreciated that in an alternative embodiment, the image generation module 230 can be configured to perform S203.
In summary, according to the microwave microscopic imaging method, the microwave microscopic imaging device, and the microwave microscopic imaging system provided in the embodiments of the present invention, the first photoacoustic signal and the second photoacoustic signal before and after heating the target excitation point in the multiple excitation points included in the sample to be measured by using pulsed microwaves are obtained, and then the microwave absorption coefficient of the target excitation point is determined according to the first photoacoustic signal and the second photoacoustic signal, so that the microwave microscopic image of the sample to be measured is obtained according to the position coordinate of each excitation point and the corresponding microwave absorption coefficient. Since the difference value of the second photoacoustic signal and the first photoacoustic signal includes the temperature change of the target excitation point caused by the pulse microwave, the microwave absorption coefficient can be determined according to the second photoacoustic signal and the first photoacoustic signal; and because the laser can be focused, the area of a light spot irradiated to an excitation point is reduced, so that more excitation points can be measured by the sample to be measured, the image resolution is improved, and the microwave microscopic imaging is realized.
In the embodiments provided in the present application, it should be understood that the disclosed apparatus and method can be implemented in other ways. The apparatus embodiments described above are merely illustrative, and for example, the flowchart and block diagrams in the figures illustrate the architecture, functionality, and operation of possible implementations of apparatus, methods and computer program products according to various embodiments of the present invention. In this regard, each block in the flowchart or block diagrams may represent a module, segment, or portion of code, which comprises one or more executable instructions for implementing the specified logical function(s). It should also be noted that, in some alternative implementations, the functions noted in the block may occur out of the order noted in the figures. For example, two blocks shown in succession may, in fact, be executed substantially concurrently, or the blocks may sometimes be executed in the reverse order, depending upon the functionality involved. It will also be noted that each block of the block diagrams and/or flowchart illustration, and combinations of blocks in the block diagrams and/or flowchart illustration, can be implemented by special purpose hardware-based systems which perform the specified functions or acts, or combinations of special purpose hardware and computer instructions.
In addition, the functional modules in the embodiments of the present invention may be integrated together to form an independent part, or each module may exist separately, or two or more modules may be integrated to form an independent part.
The functions, if implemented in the form of software functional modules and sold or used as a stand-alone product, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present invention may be embodied in the form of a software product, which is stored in a storage medium and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the steps of the method according to the embodiments of the present invention. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk, or an optical disk, and various media capable of storing program codes.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. A microwave microscopic imaging method is applied to a microwave microscopic imaging system, and comprises the following steps:
acquiring a first photoacoustic signal and a second photoacoustic signal, wherein the first photoacoustic signal and the second photoacoustic signal are respectively photoacoustic signals before and after heating a target excitation point in a plurality of excitation points included in a sample to be measured by using pulse microwaves;
determining a microwave absorption coefficient of the target excitation point according to the first photoacoustic signal and the second photoacoustic signal, wherein the microwave absorption coefficient corresponds to the target excitation point;
obtaining a microwave microscopic image of the sample to be detected according to the position coordinate of each excitation point and the corresponding microwave absorption coefficient;
the step of determining the microwave absorption coefficient of the target excitation point from the first photoacoustic signal and the second photoacoustic signal comprises:
determining the temperature variation of the target excitation point according to the first photoacoustic signal and the second photoacoustic signal; the first photoacoustic signal, the second photoacoustic signal, and the temperature variation satisfy the following equation:
wherein, PA 0 Is the first photoacoustic signal, and PA 0 ∝α(AT 0 +B)，PA 1 Is the second photoacoustic signal, and PA 1 ∝α[A(T 0 +ΔT)+B]Alpha is a preset laser absorption coefficient, A is a preset first coefficient, B is a preset second coefficient, and T is 0 Δ T is the temperature variation for the pre-acquired indoor ambient temperature;
determining the microwave absorption coefficient of the target excitation point based on the temperature variation and a preset parameter set, wherein the preset parameter set comprises a preset time length for microwave radiation to last, a microwave repetition frequency, microwave energy, the density of a sample to be detected and the specific heat capacity of the sample to be detected, and the temperature variation, the preset parameter set and the microwave absorption coefficient satisfy the following formula:
wherein Δ T is the temperature variation, T is a preset duration of microwave radiation, f is the microwave repetition frequency, β is the microwave absorption coefficient, H is the microwave energy, ρ is the density of the sample to be measured, C p Is the specific heat capacity of the sample to be measured.
2. The microwave microscopy imaging method as claimed in claim 1, wherein the microwave microscopy imaging system comprises a processor, a laser generation unit, a microwave generation unit, an ultrasonic detection unit and a coupling unit, the sample to be tested is disposed in the coupling unit, the processor is electrically connected with the laser generation unit, the microwave generation unit and the ultrasonic detection unit respectively, and the step of acquiring the first photoacoustic signal and the second photoacoustic signal comprises:
controlling the laser generating unit to emit pulse laser to the target excitation point;
receiving a first photoacoustic signal acquired by the ultrasonic detection unit;
after the first preset time, controlling the microwave generating unit to emit pulsed microwaves to the target excitation point for a preset time to heat the target excitation point;
and controlling the laser generating unit to emit the pulse laser to the target excitation point again, and receiving a second photoacoustic signal acquired by the ultrasonic detection unit.
3. A microwave microscopic imaging apparatus, applied to a microwave microscopic imaging system, the apparatus comprising:
the photoacoustic signal acquisition module is used for acquiring a first photoacoustic signal and a second photoacoustic signal, wherein the first photoacoustic signal and the second photoacoustic signal are respectively photoacoustic signals before and after heating a target excitation point in a plurality of excitation points contained in a sample to be measured by using pulse microwaves;
a microwave absorption coefficient determining module, configured to determine a microwave absorption coefficient of the target excitation point according to the first photoacoustic signal and the second photoacoustic signal, where the microwave absorption coefficient corresponds to the target excitation point;
the image generation module is used for obtaining a microwave microscopic image of the sample to be detected according to the position coordinates of each excitation point and the corresponding microwave absorption coefficient;
the microwave absorption coefficient determining module is configured to determine a temperature variation of the target excitation point according to the first photoacoustic signal and the second photoacoustic signal, where the first photoacoustic signal, the second photoacoustic signal, and the temperature variation satisfy the following equation:
wherein, PA 0 Is the first photoacoustic signal, and PA 0 ∝α(AT 0 +B)，PA 1 Is the second photoacoustic signal, and PA 1 ∝α[A(T 0 +ΔT)+B]Alpha is a preset laser absorption coefficient, A is a preset first coefficient, B is a preset second coefficient, and T is 0 Δ T is the temperature variation for the pre-acquired indoor ambient temperature;
the microwave absorption coefficient determining module is further configured to determine a microwave absorption coefficient of the target excitation point based on the temperature variation and a preset parameter set, where the preset parameter set includes a preset duration of microwave radiation, a microwave repetition frequency, a microwave energy, a density of a sample to be detected, and a specific heat capacity of the sample to be detected, and the temperature variation, the preset parameter set, and the microwave absorption coefficient satisfy a formula:
wherein Δ T is the temperature variation, T is a preset duration of the microwave radiation, f is the microwave repetition frequency, β is the microwave absorption coefficient, H is the microwave energy, ρ is the density of the sample to be measured, C p Is the specific heat capacity of the sample to be measured.
4. A microwave microscopy imaging system, characterized in that the microwave microscopy imaging system comprises a processor for performing the microwave microscopy imaging method according to any one of claims 1-2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910774539.5A CN110464350B (en) | 2019-08-21 | 2019-08-21 | Microwave microscopic imaging method, device and system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910774539.5A CN110464350B (en) | 2019-08-21 | 2019-08-21 | Microwave microscopic imaging method, device and system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110464350A CN110464350A (en) | 2019-11-19 |
| CN110464350B true CN110464350B (en) | 2023-03-24 |
Family
ID=68513283
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910774539.5A Active CN110464350B (en) | 2019-08-21 | 2019-08-21 | Microwave microscopic imaging method, device and system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110464350B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683807A (en) * | 2020-12-10 | 2021-04-20 | 广东省医疗器械研究所 | Tissue slice imaging method and imaging system |
| CN120043653B (en) * | 2025-04-17 | 2025-08-05 | 西湖大学 | Temperature measurement method, device and storage medium |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5713356A (en) * | 1996-10-04 | 1998-02-03 | Optosonics, Inc. | Photoacoustic breast scanner |
| US6567688B1 (en) * | 1999-08-19 | 2003-05-20 | The Texas A&M University System | Methods and apparatus for scanning electromagnetically-induced thermoacoustic tomography |
| CN102269717B (en) * | 2011-04-27 | 2012-12-26 | 华南师范大学 | Ultrashort pulse microwave thermoacoustic imaging method and device |
| CN103096810A (en) * | 2011-07-14 | 2013-05-08 | 松下电器产业株式会社 | Analysis device and analysis method |
| JP2013128759A (en) * | 2011-11-22 | 2013-07-04 | Fujifilm Corp | Photoacoustic signal processing device and method |
| JP5779567B2 (en) * | 2011-11-22 | 2015-09-16 | 富士フイルム株式会社 | Photoacoustic signal processing apparatus and method |
| CN105259426A (en) * | 2014-07-18 | 2016-01-20 | 中国科学院沈阳自动化研究所 | Radiation field spatial distribution measurement apparatus and method of thermo-acoustic effect |
| CN107713990A (en) * | 2017-10-31 | 2018-02-23 | 华南师范大学 | A kind of thermoacoustic, optoacoustic, ultrasonic three mode tumor of breast detection means and method |
-
2019
- 2019-08-21 CN CN201910774539.5A patent/CN110464350B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110464350A (en) | 2019-11-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5496098B2 (en) | Subject information acquisition apparatus and control method thereof | |
| JP2011005042A (en) | Photoacoustic imaging apparatus and photoacoustic imaging method | |
| JP6946307B2 (en) | Information acquisition device and signal processing method | |
| CN105572049B (en) | Optoacoustic quantifies elastograph imaging method and device | |
| CN116421304A (en) | Method and apparatus for selective photothermolysis | |
| EP1161661A1 (en) | Methods and equipment for the measurement of the three-dimensional distribution of the temperatures within dielectric means | |
| CN104334088A (en) | Laser-induced ultrasonic wave apparatus and method | |
| CN110464350B (en) | Microwave microscopic imaging method, device and system | |
| Moilanen et al. | Photo-acoustic excitation and optical detection of fundamental flexural guided wave in coated bone phantoms | |
| CN106725348A (en) | A kind of absorber absorption coefficient measurement and the simultaneously lossless detection method and its device of photoacoustic imaging | |
| Li et al. | High‐speed intra‐operative assessment of breast tumour margins by multimodal ultrasound and photoacoustic tomography | |
| US9566006B2 (en) | Object information acquisition apparatus | |
| Kim et al. | Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach | |
| KR20150096272A (en) | Method of controlling temperature of tissue and apparatus of teperature control using the same | |
| JP6486085B2 (en) | Photoacoustic wave measuring device | |
| Soroushian et al. | Study of laser-induced thermoelastic deformation of native and coagulated ex-vivo bovine liver tissues for estimating their optical and thermomechanical properties | |
| Rui et al. | Multiple information extracted from photoacoustic radio-frequency signal and the application on tissue classification | |
| KR20170074171A (en) | Photoacoustic apparatus, information acquiring apparatus, information acquiring method, and program | |
| JP6598528B2 (en) | Subject information acquisition apparatus and subject information acquisition method | |
| US20160206246A1 (en) | Object information acquiring apparatus and object information acquisition method | |
| Jian et al. | Frequency domain analysis of multiwavelength photoacoustic signals for differentiating tissue components | |
| KR20150067586A (en) | Method of controlling temperature of tissue and apparatus of teperature control using the same | |
| JP6619379B2 (en) | Component concentration measuring apparatus and method | |
| CN120043653B (en) | Temperature measurement method, device and storage medium | |
| US20250116547A1 (en) | Thermoacoustic sensor with fluid mixing for measurement of acoustic power of ultrasound transducers over a widefrequency range |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |