CN110455586A - A kind of continuous sampling method suitable for the production of scale biological products - Google Patents
A kind of continuous sampling method suitable for the production of scale biological products Download PDFInfo
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- CN110455586A CN110455586A CN201910811670.4A CN201910811670A CN110455586A CN 110455586 A CN110455586 A CN 110455586A CN 201910811670 A CN201910811670 A CN 201910811670A CN 110455586 A CN110455586 A CN 110455586A
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- 238000005070 sampling Methods 0.000 title claims abstract description 107
- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 24
- 239000000084 colloidal system Substances 0.000 claims abstract description 149
- 238000003825 pressing Methods 0.000 claims abstract description 93
- 239000000523 sample Substances 0.000 claims abstract description 36
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 230000002572 peristaltic effect Effects 0.000 claims description 19
- 238000010926 purge Methods 0.000 claims description 17
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 235000009854 Cucurbita moschata Nutrition 0.000 claims description 10
- 240000001980 Cucurbita pepo Species 0.000 claims description 10
- 235000009852 Cucurbita pepo Nutrition 0.000 claims description 10
- 235000020354 squash Nutrition 0.000 claims description 10
- 239000003899 bactericide agent Substances 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 7
- 239000003292 glue Substances 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000002045 lasting effect Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 5
- 230000010412 perfusion Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229940030225 antihemorrhagics Drugs 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000000025 haemostatic effect Effects 0.000 description 1
- 239000012943 hotmelt Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 244000309459 oncolytic virus Species 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004801 process automation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1418—Depression, aspiration
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- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to biological products production technical fields, in particular a kind of continuous sampling method suitable for the production of scale biological products, including sampling cavity, the bottom of the sampling cavity is connected with sampling probe by the first sterile colloid hose, the first pressing type switch valve is installed on the first sterile colloid hose, the input terminal of the first aseptic gas filter is connected at the right center of the sampling cavity by the second sterile colloid hose, the output end of the first aseptic gas filter is connected with vacuum pump pipeline connector by the sterile colloid hose of third, the end of the sampling cavity is connected to the output end of rotary multi-way valve by hard sterile pipes, the present invention realizes the full-automation of liquid sampling process by design, avoid the artificial participation in closed system, effectively improving operating efficiency, continuously exist in implementation process Aseptic while line samples, during effective guarantee whole operation.
Description
Technical field
The present invention relates to biological products production technical field, specially a kind of company suitable for the production of scale biological products
Continuous sampling method.
Background technique
New bio drug, the viral vectors used such as variant cell treatment product (UCAR-T), cell therapy product (are used
In gene transfer etc.), oncolytic virus etc., it is positive to accelerate to be clinical application and large-scale production from Laboratory Conversion, as drug or
Suspension cell as production carrier is during large-scale production, using the closed liquid filling production system of large capacity, in order to
It ensures that the closed liquid filling production system of large capacity reaches the productivity effect of optimization, needs in the online production system of acquisition in real time
Sample is used for the detection of all types of index parameters, adjusts each parameter in production process, more in traditional small-scale liquid filling system
Sample collection by the way of manual in carry out system, in each sample collection procedure, operator need to break total system
Closure, carry out artificial sample extraction with special syringe or sampler connection special tube line, complete sample manually extract
Operator completes system-wide closing with modes such as hot melt/haemostatic clamp again afterwards, and traditional manual sampling mode exists apparent
Defect: 1) it in sampling process, needs to break system-wide closure, seriously affects the aseptic in perfusion system, bring
The great risk of contamination of products during large-scale production；2) hand sample process is cumbersome, single-point single sampling need spend compared with
For a long time, the sampling of multiple spot serialization during large-scale production can not be adapted to；3) in the perfusion system production process of scale
In, total system needs to realize the production of whole process automation in clean environment, and traditional sampling mode can not be realized effectively automatically
Sampling, in order to meet the continuous sampling of multiple spot in scale perfusion system, it is desirable to provide a kind of new way, to meet: 1)
The on-line continuous sampling for adapting to scale perfusion production system, meets the needs of multiple spot continuous and automatic sampling；2) it avoids sampling
The destruction of production system closure in the process ensures the aseptic of closed system.
In conclusion the present invention is solved by designing a kind of continuous sampling method produced suitable for scale biological products
The problem of certainly existing.
Summary of the invention
The purpose of the present invention is to provide a kind of continuous sampling methods suitable for the production of scale biological products, to solve
The problems mentioned above in the background art.
To achieve the above object, the invention provides the following technical scheme:
A kind of continuous sampling method suitable for the production of scale biological products, including sampling cavity, the sampling cavity
Bottom sampling probe is connected with by the first sterile colloid hose, the first squash type is installed on the first sterile colloid hose
Switch valve by the second sterile colloid hose is connected to the first aseptic gas filter at the right center of the sampling cavity
Input terminal, the output end of the first aseptic gas filter are connected with vacuum pump pipeline by the sterile colloid hose of third and connect
Head, is equipped with the second pressing type switch valve on the second sterile colloid hose, the end of the sampling cavity by hard without
Tube road is connected to the output end of rotary multi-way valve, and the right side valve port of the rotary multi-way valve is soft by the 4th sterile colloid
Pipe is connected on the output end of the second aseptic gas filter, and the input terminal of the second aseptic gas filter passes through the 5th nothing
Bacterium colloid hose is connected on purging compressed air hose pipeline connector, and the 5th sterile colloid hose is equipped with third squash type and opens
Valve is closed, the left side valve port of the rotary multi-way valve is connected on sterilizing pipe joint by the 6th sterile colloid hose, described
6th sterile colloid hose is equipped with the 4th pressing type switch valve, and the upper left side of the sampling cavity is soft by the 7th sterile colloid
Pipe is connected on the input terminal of third aseptic gas filter, and the output end of the third aseptic gas filter passes through the 9th nothing
Bacterium colloid hose is connected with baroceptor pipe joint, and the 7th sterile colloid hose is equipped with the 5th pressing type switch
Valve, the lower section of the 7th sterile colloid hose and is located on sampling cavity and by the 9th sterile colloid hose is connected to sample
On sampling line connector, the 9th sterile colloid hose is equipped with the 6th pressing type switch valve.
Preferably, the vacuum pump pipeline connector, purging compressed air hose pipeline connector, sterilizing pipe joint and sample sampling
Pipe joint is all made of the self-locking tracheae quick coupling of c formula.
Preferably, the vacuum pump pipeline connector is connected to the output end of vacuum pump, and the purging compressed air line connects
Head is connected to the output end of air compressor, and the output end of peristaltic pump and peristaltic pump are connected on the sterilizing pipe joint
Output end is connected on the cavity equipped with bactericidal agent, and described sampling line connector is connected on productive culture primary tank, described
Baroceptor is installed on baroceptor pipe joint.
Preferably, the rotary multi-way valve is rotary three-way valve and model BX44W.
Preferably, the described first sterile colloid hose, the second sterile colloid hose, hard sterile pipes, the 7th sterile glue
Matter hose and the 9th sterile colloid hose are respectively connectivity structure with sampling cavity.
Preferably, comprising the following steps:
Inner cavity and inner conduit sterilizing and washing: S1 is connected to the output end of peristaltic pump and compacted on sterilizing pipe joint
Under the action of the output end of dynamic pump is connected on the cavity equipped with bactericidal agent, opening for peristaltic pump and the 4th pressing type switch valve is opened
Closing valve, rotating rotary multi-way valve makes the 6th sterile colloid hose pass through hard sterile pipes and sampling cavity connected state
State simultaneously closes off, and second on the first pressing type switch valve, the second sterile colloid hose on the first sterile colloid hose squeezes
Formula switch valve, the third pressing type switch valve on the 5th sterile colloid hose, the 5th squash type on the 7th sterile colloid hose
The controlled valve of switch valve and the 6th pressing type switch valve on the 9th sterile colloid hose is led to bactericidal agent by peristaltic pump
It crosses the 6th sterile colloid hose and inputs the inside of sampling cavity along hard sterile pipes, and keep setting time；
Sterilized solution discharge: S2 closes the controlled valve of peristaltic pump and the 4th pressing type switch valve, in this sampling line connector
It is connected on productive culture primary tank and purging compressed air hose pipeline connector is connected to the effect of the output end of air compressor
Under, the first pressing type switch valve opened on the first sterile colloid hose is opened with the third squash type on the 5th sterile colloid hose
The controlled valve for closing valve, simultaneously closes off controlled valve, the second nothing of the 6th pressing type switch valve on the 9th sterile colloid hose
The switch valve of the second pressing type switch valve on bacterium colloid hose and the 5th pressing type switch valve on the 7th sterile colloid hose
Door, rotating rotary multi-way valve makes the 5th sterile colloid hose by hard sterile pipes and sampling cavity connected state, opens
It opens air compressor generation compressed gas to purge the 5th sterile colloid hose, hard sterile pipes and sampling cavity, arrange
Remaining sterilized liquid out ensures sufficient purge time, and internal duct and cavity are restored to drying regime；
S3, sample sampling prepare: closing the first pressing type switch valve, the second pressing type switch valve, third pressing type switch
Valve, the 4th pressing type switch valve, the 5th pressing type switch valve) and the 6th pressing type switch valve controlled valve；
Sample sampling: S4 is connected in the output end that vacuum pump pipeline connector is connected to vacuum pump with this sampling line connector
Under the working condition on productive culture primary tank, the 6th pressing type switch valve and second on the 9th sterile colloid hose is opened
The controlled valve of the second pressing type switch valve on sterile colloid hose, to make the second sterile colloid hose and the 9th sterile glue
Matter hose is respectively the working condition being connected to sampling cavity, opens vacuum pump, vacuum pump work will be in productive culture primary tank
Liquid is drawn in sampling cavity by negative pressure of vacuum, closes the 6th pressing type switch valve on the 9th sterile colloid hose and the
The controlled valve of the second pressing type switch valve on two sterile colloid hoses；
S5, transfer of the sample to external detection container: outer samples container is transferred to below sampling probe, opens the first nothing
The third pressing type switch threshold switch valve on the first pressing type switch valve and the 5th sterile colloid hose on bacterium colloid hose
Door opens air compressor generation compressed gas and by the 5th sterile colloid hose and enters sampling along hard sterile pipes
Cavity is transferred to external samples container for inside cavity sample liquid is sampled；
S6 is cleaned after sampling: repeating S1, the operation in S2；
S7, lasting cleaning are kept: the controlled valve of the 5th pressing type switch valve on the sterile colloid hose of sample the 7th is opened,
When being less than setting value with the pressure difference of environment, the third pressing type switch threshold switch valve on the 5th sterile colloid hose is opened
Door opens air compressor generation compressed gas and by the 5th sterile colloid hose and enters sampling along hard sterile pipes
Cavity improves sample chamber and lifts pressure, and the third squash type closed on the sterile colloid hose in compressed gas channel i.e. the five or five is opened
Close threshold switch valve.
Compared with prior art, the beneficial effects of the present invention are:
1, in of the invention, main body culture vessel and outside when large-scale production samples link in the process are realized by design
The stringent isolation of environment has effectively achieved main body culture vessel asepsis ring condition and keeps and ensure.
2, in the present invention, the full-automation of liquid sampling process is realized by designing, is avoided artificial in closed system
It participates in, operating efficiency is being effectively improved, in implementation process while continuous on-line period, during effective guarantee whole operation
Aseptic.
Detailed description of the invention
Fig. 1 is the whole schematic diagram structural schematic diagram of the present invention；
Fig. 2 is overall flow structural schematic diagram of the present invention.
In figure: 1- samples cavity, the sterile colloid hose of 2- first, 3- sampling probe, the first pressing type switch of 4- valve, 5- second
Sterile colloid hose, the first aseptic gas of 6- filter, the sterile colloid hose of 7- third, 8- vacuum pump pipeline connector, 9- second
Pressing type switch valve, 10- hard sterile pipes, the rotary multi-way valve of 11-, the sterile colloid hose of 12- the 4th, 13- second are sterile
Pneumatic filter, the sterile colloid hose of 14- the 5th, 15- purging compressed air hose pipeline connector, 16- third pressing type switch valve,
The sterile colloid hose of 17- the 6th, 18- sterilizing pipe joint, the 4th pressing type switch valve of 19-, the sterile colloid hose of 20- the 7th,
21- third aseptic gas filter, the sterile colloid hose of 22- the 9th, 23- baroceptor pipe joint, the 5th squash type of 24-
Switch valve, the sterile colloid hose of 25- the 9th, 26- sample sampling line connector, the 6th pressing type switch valve of 27-.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
The present invention provides a kind of technical solution referring to FIG. 1-2:
A kind of continuous sampling method suitable for the production of scale biological products, including sampling cavity 1, sampling cavity 1
Bottom is connected with sampling probe 3 by the first sterile colloid hose 2, is equipped with the first squash type on the first sterile colloid hose 2 and opens
Valve 4 is closed, samples and the defeated of first aseptic gas filter 6 is connected to by the second sterile colloid hose 5 at the right center of cavity 1
Entering end, the output end of the first aseptic gas filter 6 is connected with vacuum pump pipeline connector 8 by the sterile colloid hose 7 of third, the
Second pressing type switch valve 9 is installed, the end for sampling cavity 1 is connected by hard sterile pipes 10 on two sterile colloid hoses 5
The output end in rotary multi-way valve 11 is connect, the right side valve port of rotary multi-way valve 11 is connected by the 4th sterile colloid hose 12
On the output end of the second aseptic gas filter 13, the input terminal of the second aseptic gas filter 13 passes through the 5th sterile colloid
Hose 14 is connected on purging compressed air hose pipeline connector 15, and the 5th sterile colloid hose 12 is equipped with third pressing type switch valve
16, the left side valve port of rotary multi-way valve 11 is connected on sterilizing pipe joint 18 by the 6th sterile colloid hose 17, and the 6th
Sterile colloid hose 17 is equipped with the 4th pressing type switch valve 19, and the upper left side of sampling cavity 1 passes through the 7th sterile colloid hose
20 are connected on the input terminal of third aseptic gas filter 21, and the output end of third aseptic gas filter 21 passes through the 9th nothing
Bacterium colloid hose 22 is connected with baroceptor pipe joint 23, and the 7th sterile colloid hose 20 is equipped with the 5th pressing type switch
Valve 24, the lower section of the 7th sterile colloid hose 20 and being located on sampling cavity 1 are connected to by the 9th sterile colloid hose 25
On sample sampling line connector 26, the 9th sterile colloid hose 25 is equipped with the 6th pressing type switch valve 27.
Workflow of the present invention: it is connected to the output end of vacuum pump in vacuum pump pipeline connector 8, purges compressed air line
Connector 15 is connected to the output end of air compressor, and the output end and peristaltic pump of peristaltic pump are connected on the pipe joint 18 that sterilizes
Output end be connected on the cavity equipped with bactericidal agent, this sampling line connector 26 is connected on productive culture primary tank, air pressure
Baroceptor and the first sterile colloid hose 2, the second sterile colloid hose 5, hard are installed on sensor tube pipeline connector 23
Matter sterile pipes 10, the 7th sterile colloid hose 20 and the 9th sterile colloid hose 25 are respectively connectivity structure with sampling cavity 1
Working condition under, the whole continuous sampling method specific steps of this programme include the following:
S1, inner cavity and inner conduit sterilizing and washing: be connected on sterilizing pipe joint 18 output end of peristaltic pump and
Under the action of the output end of peristaltic pump is connected on the cavity equipped with bactericidal agent, peristaltic pump and the 4th pressing type switch valve 19 are opened
Controlled valve, rotate rotary multi-way valve 11 make the 6th sterile colloid hose 17 pass through hard sterile pipes 10 and sampling cavity
1 connected state of body, simultaneously closes off, the first pressing type switch valve 4, the second sterile colloid hose 5 on the first sterile colloid hose 2
On the second pressing type switch valve 9, the third pressing type switch valve 16 on the 5th sterile colloid hose 12, the 7th sterile colloid it is soft
The switch of the 5th pressing type switch valve 24 on pipe 20 and the 6th pressing type switch valve 27 on the 9th sterile colloid hose 25
Valve inputs sampling cavity by peristaltic pump by bactericidal agent by the 6th sterile colloid hose 17 and along hard sterile pipes 10
The inside of body 1, and keep setting time；
Sterilized solution discharge: S2 closes the controlled valve of peristaltic pump and the 4th pressing type switch valve 19, connects in this sampling line
First 26 are connected on productive culture primary tank and purge the output end that compressed air hose pipeline connector 15 is connected to air compressor
Under the action of, open the on the first pressing type switch valve 4 and the 5th sterile colloid hose 12 on the first sterile colloid hose 2
The controlled valve of three pressing type switch valves 16 simultaneously closes off the 6th pressing type switch valve 27 on the 9th sterile colloid hose 25
The 5th on the second pressing type switch valve 9 and the 7th sterile colloid hose 20 on controlled valve, the second sterile colloid hose 5 squeezes
The controlled valve of pusher-type switch valve 24, rotating rotary multi-way valve 11 makes the 5th sterile colloid hose 12 pass through hard sterile tube
Road 10 and sampling 1 connected state of cavity, open air compressor generate compressed gas to the 5th sterile colloid hose 12, hard without
Tube road 10 is purged with sampling cavity 1, and remaining sterilized liquid is discharged, and sufficient purge time is ensured, by internal duct
Drying regime is restored to cavity；
S3, sample sampling prepare: closing the first pressing type switch valve 4, the second pressing type switch valve 9, third squash type and open
Close the controlled valve of valve 16, the 4th pressing type switch valve 19, the 5th pressing type switch valve 24 and the 6th pressing type switch valve 27；
Sample sampling: S4 is connected to the output end of vacuum pump in vacuum pump pipeline connector 8 and this sampling line connector 26 connects
It connects under the working condition on productive culture primary tank, opens the 6th pressing type switch valve 27 on the 9th sterile colloid hose 25
With the controlled valve of the second pressing type switch valve 9 on the second sterile colloid hose 5, to make the second sterile 5 He of colloid hose
9th sterile colloid hose 25 is respectively the working condition being connected to sampling cavity 1, opens vacuum pump, and vacuum pump work will produce
Liquid is drawn in sampling cavity 1 by negative pressure of vacuum in culture primary tank, closes the 6th on the 9th sterile colloid hose 25
The controlled valve of pressing type switch valve 27 and the second pressing type switch valve 9 on the second sterile colloid hose 5；
Transfer of the sample to external detection container: outer samples container is transferred to 3 lower section of sampling probe, opens first by S5
The first pressing type switch valve 4 on sterile colloid hose 2 and the third pressing type switch valve on the 5th sterile colloid hose 12
16 controlled valves open air compressor and generate compressed gas by the 5th sterile colloid hose 12 and along hard sterile tube
Road 10 enters sampling cavity 1 and is transferred to external samples container for 1 internal specimen liquid of cavity is sampled；
S6 is cleaned after sampling: repeating S1, the operation in S2；
S7, lasting cleaning are kept: opening the switch of the 5th pressing type switch valve 24 on the sterile colloid hose 20 of sample the 7th
Valve opens the third pressing type switch valve on the 5th sterile colloid hose 12 when being less than setting value with the pressure difference of environment
16 controlled valves open air compressor and generate compressed gas by the 5th sterile colloid hose 12 and along hard sterile tube
Road 10 enters sampling cavity 1, improves sample chamber and lifts pressure, closes on the sterile colloid hose 12 in compressed gas channel i.e. the five or five
16 controlled valve of third pressing type switch valve.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of continuous sampling method suitable for the production of scale biological products, including sampling cavity (1), it is characterised in that:
The bottom of sampling cavity (1) is connected with sampling probe (3) by the first sterile colloid hose (2), the first sterile colloid
First pressing type switch valve (4) is installed on hose (2), passes through the second sterile glue at the right center of sampling cavity (1)
Matter hose (5) is connected to the input terminal of the first aseptic gas filter (6), the output of the first aseptic gas filter (6)
End is connected with vacuum pump pipeline connector (8) by the sterile colloid hose (7) of third, is pacified on the second sterile colloid hose (5)
Equipped with the second pressing type switch valve (9), the end of sampling cavity (1) is connected to rotatably by hard sterile pipes (10)
The right side valve port of the output end of multi-way valve (11), the rotary multi-way valve (11) passes through the 4th sterile colloid hose (12) connection
On the output end of the second aseptic gas filter (13), the input terminal of the second aseptic gas filter (13) passes through the 5th
Sterile colloid hose (14) is connected on purging compressed air hose pipeline connector (15), sets on the 5th sterile colloid hose (12)
There is third pressing type switch valve (16), the left side valve port of the rotary multi-way valve (11) passes through the 6th sterile colloid hose (17)
It being connected in sterilizing pipe joint (18), the 6th sterile colloid hose (17) is equipped with the 4th pressing type switch valve (19),
The upper left side of sampling cavity (1) is connected to third aseptic gas filter (21) by the 7th sterile colloid hose (20)
On input terminal, the output end of the third aseptic gas filter (21) is connected with air pressure by the 9th sterile colloid hose (22)
Sensor tube pipeline connector (23), the 7th sterile colloid hose (20) are equipped with the 5th pressing type switch valve (24), and described the
The lower section of seven sterile colloid hoses (20) and it is located in sampling cavity (1) sample is connected to by the 9th sterile colloid hose (25)
On this sampling line connector (26), the 9th sterile colloid hose (25) is equipped with the 6th pressing type switch valve (27).
2. a kind of continuous sampling method suitable for the production of scale biological products according to claim 1, feature exist
In: the vacuum pump pipeline connector (8), purging compressed air hose pipeline connector (15), sterilizing pipe joint (18) and sample sampling
Pipe joint (26) is all made of the self-locking tracheae quick coupling of c formula.
3. a kind of continuous sampling method suitable for the production of scale biological products according to claim 1, feature exist
In: the vacuum pump pipeline connector (8) is connected to the output end of vacuum pump, purging compressed air hose pipeline connector (15) connection
In the output end of air compressor, the defeated of the output end of peristaltic pump and peristaltic pump is connected on sterilizing pipe joint (18)
Outlet is connected on the cavity equipped with bactericidal agent, and described sampling line connector (26) is connected on productive culture primary tank, institute
It states and baroceptor is installed on baroceptor pipe joint (23).
4. a kind of continuous sampling method suitable for the production of scale biological products according to claim 1, feature exist
In: the rotary multi-way valve (11) is rotary three-way valve and model BX44W.
5. a kind of continuous sampling method suitable for the production of scale biological products according to claim 1, feature exist
In: the first sterile colloid hose (2), the second sterile colloid hose (5), hard sterile pipes (10), the 7th sterile colloid
Hose (20) and the 9th sterile colloid hose (25) are respectively connectivity structure with sampling cavity (1).
6. a kind of continuous sampling method suitable for the production of scale biological products according to claim 1, feature exist
In, comprising the following steps:
Inner cavity and inner conduit sterilizing and washing: S1 is connected to the output end of peristaltic pump and compacted in sterilizing pipe joint (18)
Under the action of the output end of dynamic pump is connected on the cavity equipped with bactericidal agent, peristaltic pump and the 4th pressing type switch valve (19) are opened
Controlled valve, rotate rotary multi-way valve (11) make the 6th sterile colloid hose (17) by hard sterile pipes (10) with
Cavity (1) connected state is sampled, is simultaneously closed off, the first pressing type switch valve (4) on the first sterile colloid hose (2), second
The third pressing type switch on the second pressing type switch valve (9), the 5th sterile colloid hose (12) on sterile colloid hose (5)
On valve (16), the 5th pressing type switch valve (24) on the 7th sterile colloid hose (20) and the 9th sterile colloid hose (25)
The 6th pressing type switch valve (27) controlled valve, by peristaltic pump by bactericidal agent by the 6th sterile colloid hose (17) simultaneously
And the inside of sampling cavity (1) is inputted along hard sterile pipes (10), and keep setting time；
Sterilized solution discharge: S2 closes the controlled valve of peristaltic pump and the 4th pressing type switch valve (19), in this sampling line connector
(26) it is connected on productive culture primary tank and purging compressed air hose pipeline connector (15) is connected to the output of air compressor
Under the action of end, the first pressing type switch valve (4) and the 5th sterile colloid hose on the first sterile colloid hose (2) are opened
(12) controlled valve of the third pressing type switch valve (16) on, the 6th simultaneously closed off on the 9th sterile colloid hose (25) squeeze
The controlled valve of pusher-type switch valve (27), the second pressing type switch valve (9) on the second sterile colloid hose (5) and the 7th are sterile
The controlled valve of the 5th pressing type switch valve (24) on colloid hose (20), rotating rotary multi-way valve (11) makes the 5th nothing
Bacterium colloid hose (12) opens air compressor and generates pressure by hard sterile pipes (10) and sampling cavity (1) connected state
Contracting gas purges the 5th sterile colloid hose (12), hard sterile pipes (10) and sampling cavity (1), is discharged remaining
Sterilized liquid ensures sufficient purge time, and internal duct and cavity are restored to drying regime；
S3, sample sampling prepare: closing the first pressing type switch valve (4), the second pressing type switch valve (9), third squash type and open
Close valve (16), the 4th pressing type switch valve (19), the 5th pressing type switch valve (24)) and the 6th pressing type switch valve (27) open
Closing valve；
Sample sampling: S4 is connected to the output end of vacuum pump in vacuum pump pipeline connector (8) and this sampling line connector (26) connects
It connects under the working condition on productive culture primary tank, opens the 6th pressing type switch valve on the 9th sterile colloid hose (25)
(27) controlled valve of the second pressing type switch valve (9) and on the second sterile colloid hose (5), to make the second sterile colloid
Hose (5) and the 9th sterile colloid hose (25) are respectively the working condition being connected to sampling cavity (1), open vacuum pump, very
Liquid in productive culture primary tank is drawn in sampling cavity (1) by empty pump work by negative pressure of vacuum, closes the 9th sterile glue
The second pressing type switch valve on the 6th pressing type switch valve (27) and the second sterile colloid hose (5) on matter hose (25)
(9) controlled valve；
S5, transfer of the sample to external detection container: outer samples container is transferred to below sampling probe (3), opens the first nothing
The third squash type on the first pressing type switch valve (4) and the 5th sterile colloid hose (12) on bacterium colloid hose (2) is opened
Valve (16) controlled valve is closed, air compressor is opened and generates compressed gas by the 5th sterile colloid hose (12) and along hard
Matter sterile pipes (10) enter sampling cavity (1) and sampling cavity (1) internal specimen liquid are transferred to external samples container；
S6 is cleaned after sampling: repeating S1, the operation in S2；
S7, lasting cleaning are kept: opening the switch of the 5th pressing type switch valve (24) on the sterile colloid hose (20) of sample the 7th
Valve opens the third pressing type switch on the 5th sterile colloid hose (12) when being less than setting value with the pressure difference of environment
Valve (16) controlled valve opens air compressor and generates compressed gas by the 5th sterile colloid hose (12) and along hard
Sterile pipes (10) enter sampling cavity (1), improve sample chamber and lift pressure, close compressed gas channel i.e. the five or five sterile glue
Third pressing type switch valve (16) controlled valve on matter hose (12).
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112657441A (en) * | 2020-12-03 | 2021-04-16 | 英诺维尔智能科技（苏州）有限公司 | Application method of array reactor |
| CN115090622A (en) * | 2022-06-17 | 2022-09-23 | 厦门瑞尔特卫浴科技股份有限公司 | Cleaning system and cleaning method for urine test closestool |
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