CN110447655B - Sanitary insecticidal composition - Google Patents
Sanitary insecticidal composition Download PDFInfo
- Publication number
- CN110447655B CN110447655B CN201910762533.6A CN201910762533A CN110447655B CN 110447655 B CN110447655 B CN 110447655B CN 201910762533 A CN201910762533 A CN 201910762533A CN 110447655 B CN110447655 B CN 110447655B
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- Prior art keywords
- bait
- sanitary
- parts
- acetamiprid
- propoxur
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- 239000000203 mixture Substances 0.000 title claims abstract description 28
- 230000000749 insecticidal effect Effects 0.000 title claims abstract description 18
- 239000005875 Acetamiprid Substances 0.000 claims abstract description 33
- WCXDHFDTOYPNIE-RIYZIHGNSA-N (E)-acetamiprid Chemical compound N#C/N=C(\C)N(C)CC1=CC=C(Cl)N=C1 WCXDHFDTOYPNIE-RIYZIHGNSA-N 0.000 claims abstract description 32
- ISRUGXGCCGIOQO-UHFFFAOYSA-N Rhoden Chemical compound CNC(=O)OC1=CC=CC=C1OC(C)C ISRUGXGCCGIOQO-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000011159 matrix material Substances 0.000 claims abstract description 19
- 241001674044 Blattodea Species 0.000 claims abstract description 16
- 241000257303 Hymenoptera Species 0.000 claims abstract description 10
- 241000255925 Diptera Species 0.000 claims abstract description 7
- 239000004480 active ingredient Substances 0.000 claims abstract 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 12
- 239000002917 insecticide Substances 0.000 claims description 12
- 235000010413 sodium alginate Nutrition 0.000 claims description 12
- 239000000661 sodium alginate Substances 0.000 claims description 12
- 229940005550 sodium alginate Drugs 0.000 claims description 12
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 9
- 229920002125 Sokalan® Polymers 0.000 claims description 9
- 229960001631 carbomer Drugs 0.000 claims description 9
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 239000005667 attractant Substances 0.000 claims description 4
- 230000031902 chemoattractant activity Effects 0.000 claims description 4
- 239000003755 preservative agent Substances 0.000 claims description 4
- 230000002335 preservative effect Effects 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 3
- 238000009472 formulation Methods 0.000 claims description 3
- 239000000600 sorbitol Substances 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 241000233866 Fungi Species 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims description 2
- 239000000443 aerosol Substances 0.000 claims description 2
- 230000000844 anti-bacterial effect Effects 0.000 claims description 2
- 239000003963 antioxidant agent Substances 0.000 claims description 2
- 230000003078 antioxidant effect Effects 0.000 claims description 2
- 239000003899 bactericide agent Substances 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 235000000346 sugar Nutrition 0.000 claims description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 2
- 239000000796 flavoring agent Substances 0.000 claims 1
- 235000019634 flavors Nutrition 0.000 claims 1
- 239000003921 oil Substances 0.000 claims 1
- 239000003380 propellant Substances 0.000 claims 1
- 150000008163 sugars Chemical class 0.000 claims 1
- 235000019871 vegetable fat Nutrition 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 16
- 239000003795 chemical substances by application Substances 0.000 abstract description 15
- 241000607479 Yersinia pestis Species 0.000 abstract description 9
- 230000002195 synergetic effect Effects 0.000 abstract description 8
- 229940079593 drug Drugs 0.000 abstract description 5
- 238000001125 extrusion Methods 0.000 abstract description 5
- 206010059866 Drug resistance Diseases 0.000 abstract description 3
- 230000009471 action Effects 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 230000007246 mechanism Effects 0.000 abstract description 3
- 206010040880 Skin irritation Diseases 0.000 abstract description 2
- 230000001934 delay Effects 0.000 abstract description 2
- 230000003020 moisturizing effect Effects 0.000 abstract description 2
- 239000002728 pyrethroid Substances 0.000 abstract description 2
- 230000036556 skin irritation Effects 0.000 abstract description 2
- 231100000475 skin irritation Toxicity 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 40
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 18
- 241000238631 Hexapoda Species 0.000 description 14
- 230000002147 killing effect Effects 0.000 description 13
- 238000002360 preparation method Methods 0.000 description 10
- 239000000575 pesticide Substances 0.000 description 9
- 230000001018 virulence Effects 0.000 description 9
- 241000257159 Musca domestica Species 0.000 description 8
- 241000238675 Periplaneta americana Species 0.000 description 8
- 238000003756 stirring Methods 0.000 description 8
- 238000005303 weighing Methods 0.000 description 8
- 241000257226 Muscidae Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 210000003298 dental enamel Anatomy 0.000 description 5
- 239000002574 poison Substances 0.000 description 5
- 231100000614 poison Toxicity 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- 239000003085 diluting agent Substances 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 239000010413 mother solution Substances 0.000 description 4
- 231100000572 poisoning Toxicity 0.000 description 4
- 230000000607 poisoning effect Effects 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 230000004580 weight loss Effects 0.000 description 4
- 235000009161 Espostoa lanata Nutrition 0.000 description 3
- 240000001624 Espostoa lanata Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 235000012813 breadcrumbs Nutrition 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 210000000653 nervous system Anatomy 0.000 description 3
- 238000002203 pretreatment Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000005906 Imidacloprid Substances 0.000 description 2
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical compound CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 2
- 229960004373 acetylcholine Drugs 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 235000021186 dishes Nutrition 0.000 description 2
- 229940056881 imidacloprid Drugs 0.000 description 2
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000005923 long-lasting effect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 230000000946 synaptic effect Effects 0.000 description 2
- 239000004557 technical material Substances 0.000 description 2
- POPPVIRYGJQIOF-UHFFFAOYSA-N 2-acetyloxyethyl(trimethyl)azanium;3-(1-methylpyrrolidin-2-yl)pyridine Chemical compound CC(=O)OCC[N+](C)(C)C.CN1CCCC1C1=CC=CN=C1 POPPVIRYGJQIOF-UHFFFAOYSA-N 0.000 description 1
- 241000238659 Blatta Species 0.000 description 1
- 108010009685 Cholinergic Receptors Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 238000010159 Duncan test Methods 0.000 description 1
- 241000231785 Megacephala Species 0.000 description 1
- 241000952627 Monomorium pharaonis Species 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 241000238661 Periplaneta Species 0.000 description 1
- 241001078625 Plagiolepis alluaudi Species 0.000 description 1
- 241001492664 Solenopsis <angiosperm> Species 0.000 description 1
- 241000736128 Solenopsis invicta Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 102000034337 acetylcholine receptors Human genes 0.000 description 1
- 239000010775 animal oil Substances 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- VEMKTZHHVJILDY-UXHICEINSA-N bioresmethrin Chemical compound CC1(C)[C@H](C=C(C)C)[C@H]1C(=O)OCC1=COC(CC=2C=CC=CC=2)=C1 VEMKTZHHVJILDY-UXHICEINSA-N 0.000 description 1
- 235000015895 biscuits Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000000152 carbamate pesticide Substances 0.000 description 1
- 150000004657 carbamic acid derivatives Chemical class 0.000 description 1
- 229940049638 carbomer homopolymer type c Drugs 0.000 description 1
- 229940043234 carbomer-940 Drugs 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- -1 chloronicotinyl Chemical group 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 239000004521 hot fogging concentrate Substances 0.000 description 1
- 238000012613 in situ experiment Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 210000001428 peripheral nervous system Anatomy 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/006—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits insecticidal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/22—O-Aryl or S-Aryl esters thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Food Science & Technology (AREA)
- Toxicology (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a sanitary insecticidal composition, the effective components of which are propoxur and acetamiprid; the mass ratio of the propoxur to the acetamiprid is 10:1-1: 10; the content of the effective components in the composition is 0.01-5 wt%. The control objects of the sanitary insecticidal composition are ants, cockroaches and flies. The invention also provides a matrix for the insecticidal gel bait. The bait agent adopts two active ingredients with different action mechanisms, is mixed according to a proper proportion, has obvious synergistic effect compared with a single agent, can effectively prevent and treat resistant pests, and delays the occurrence and development of drug resistance; and can avoid skin irritation caused by pyrethroid medicines, and has high safety. The bait matrix provided by the invention has proper viscosity and hardness, and can be suitable for extrusion products; the bait has good moisturizing effect, does not go moldy and dry and hard after being placed for a long time, can increase the application interval, and can be widely used for preparing various baits.
Description
Technical Field
The invention belongs to the field of sanitary insecticides, and relates to a sanitary insecticidal composition containing propoxur and acetamiprid.
Background
Acetamiprid belongs to chloronicotinyl insecticides, has contact poisoning, stomach poisoning and strong osmosis, acts on a nicotine acetylcholine receptor at a synaptic part of an insect nervous system, interferes with stimulation conduction of the insect nervous system, causes the obstruction of a pathway of the insect nervous system, causes the accumulation of neurotransmitter acetylcholine at the synaptic part, and leads to the paralysis and the final death of the insect. Based on a special action mechanism, the acetamiprid can effectively kill the sanitary pests with resistance to organochlorines, organophosphates, carbamates and pyrethroids.
The propoxur belongs to carbamate pesticide, inhibits the activity of acetylcholine enzyme in the nerve center and peripheral nervous system of insects, has the functions of contact poisoning and stomach poisoning, and has high knockdown speed and long lasting period on pests. The effective component is applied to the control of flies, cockroaches and ants in the form of bait, has quick response in field application, and does not generate residue and pollution to the environment.
At present, most of effective components of fly baits and cockroach baits adopt single agents, the action mechanism is single, and metabolic resistance and target site mutation are easy to generate after long-time application, so that field application control fails. Compared with a single agent, the mixed agent can effectively prevent and control resistant pests and delay the occurrence and development of drug resistance.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a sanitary insecticidal composition which has good effect and low toxicity.
In order to achieve the purpose, the invention adopts the following technical scheme.
A sanitary insecticidal composition comprises propoxur and acetamiprid as effective components; the mass ratio of the propoxur to the acetamiprid is 10:1-1: 10; preferably 2:1 to 1: 4.
The content of the effective components in the composition is 0.01-5wt%, preferably 0.5-2 wt%.
The control objects of the sanitary insecticidal composition are ants, cockroaches and flies. E.g. Solenopsis invictaMonomorium pharaonisGerman cockroachBlattella germanicaAmerican cockroachPeriplaneta americanaHousefly, houseflyMusca domesticaLucilia sericataLucilia sericataBig head golden flyChrysomya megacephala。
The formulation of the sanitary insecticidal composition is selected from any one of but not limited to a spray, a hot fogging concentrate, an aerosol, a bait or a powder; preferably a bait.
A bait containing the sanitary insecticidal composition. The bait matrix is prepared from the following raw materials in parts by weight:
430 parts of carbomer gel, 5-15 parts of sodium alginate, 0.5-10.5 parts of calcium chloride, 250 parts of sorbitol, 15-20 parts of attractant, 1-10 parts of preservative, 25-30 parts of isopropanol, 20-30 parts of ethanol and 50-60 parts of water.
The carbomer is of type 940 or 941. The concentration of the carbomer gel is 0.5-2%.
The viscosity of the sodium alginate is 300-500mPa.s, and the M/G ratio is 2.5-3.2.
The mass ratio of the sodium alginate to the calcium chloride is 10: 1-1.4.
The attractant can be sugar, animal and vegetable oil or essence. The preservative is a bactericide or an antioxidant for resisting fungi and bacteria.
The invention has the following advantages:
the bait adopts two effective components with different chemical structures, has different target sites and enzymes, is mixed according to a proper proportion, has obvious synergistic effect compared with a single dose, can effectively prevent and treat resistant pests, and delays the occurrence and development of drug resistance; and can avoid skin irritation caused by pyrethroid medicines, and has high safety. The bait matrix provided by the invention has proper viscosity and hardness, and can be suitable for extrusion products; the bait has good moisturizing effect, does not go moldy and dry and hard after being placed for a long time, can increase the application interval, and can be widely used for preparing various baits.
Detailed Description
The present invention will be further illustrated with reference to the following examples, but the present invention is not limited to the following examples.
Example 1 propoxur and acetamiprid on Musca domestica: (Musca domestica) Combined virulence determination of
1. Preparation of feed with poison
Weighing propoxur (95% TC) and acetamiprid (96% TC) according to different mass ratios to prepare 0.1% acetone mother liquor, wherein the acetone mother liquor contains 0.1% Tween-80; then 1% honey water is used to prepare dilution liquid with series concentration, and the dilution liquid is dripped on the absorbent cotton ball until saturation, and is used as test bait.
2. Test apparatus
A square box device, 70cm × 70cm × 70 cm;
petri dishes (inner diameter 9 cm).
3. Test procedure
According to the national standard, part 7 of pesticide laboratory efficacy test and evaluation of sanitary insecticides for pesticide registration: relevant requirements of bait (GB/T13917.7-2009) tests were carried out, all in a space where the temperature and humidity were relatively stable [ temperature: (26 ± 1) ° c, relative humidity: 60% ± 10% ] is carried out. The test adopts a square box device, the test bait is placed in the center of the square box, then 50 adult flies (female: male =1: 1) which are eclosized for 3-4 days are placed in the square box, the death number of the test flies is checked after 24 hours of the test, and the death rate is calculated. Each medicament or compound is provided with 5 series concentrations, each concentration is provided with 3 times of repetition, and a soaking cotton ball is set as a blank control test.
4. Data processing
The result is analyzed and calculated by the Probit program of SPSS 20.0 software to obtain LC50And calculating the CTC of the combined virulence of the two agents. The results are shown in table 1:
TABLE 1 Combined virulence of propoxur and acetamiprid on Musca domestica
As can be seen from the data in Table 1, when the mass ratio of propoxur to acetamiprid is 4:1-1:10, the synergistic effect on the CTC of houseflies is above 120, and the synergistic effect is obvious.
Example 2 propoxur and acetamiprid on Periplaneta americana (Periplaneta americana: (Periplaneta americana) (Periplaneta americana))Periplaneta americana) Combined virulence determination of
1. Preparation of poison bait
Weighing propoxur (95% TC) and acetamiprid (96% TC) according to different mass ratios to prepare 0.1% acetone mother solution, diluting the acetone mother solution into diluents with series concentrations, adding the diluents with different concentrations into artificial feed (bread crumbs), uniformly stirring, and naturally volatilizing the acetone to obtain the test bait.
2. Test apparatus
A square box device, 70cm × 70cm × 70 cm;
petri dishes (inner diameter 9 cm).
3. Test procedure
According to the national standard, part 7 of pesticide laboratory efficacy test and evaluation of sanitary insecticides for pesticide registration: relevant requirements of bait (GB/T13917.7-2009) tests were carried out, all in a space where the temperature and humidity were relatively stable [ temperature: (26 ± 1) ° c, relative humidity: 60% ± 10% ] is carried out. A square box device is adopted for testing, 30 Periplaneta americana adults (female: male =1: 1) with the age of 10-15 days are placed into the square box device with a closed door through insect placing holes, the insect placing holes are tightly plugged, after the tested insects recover normal activities, test bait is placed in the center of the square box, a culture dish containing soaked cotton balls is placed at the same time, the death condition of the tested insects is observed day by day, the dead insects are taken out timely, the death number of the tested insects is recorded after continuous observation is carried out until the 12 th day after bait casting, and the death rate is calculated. Each medicament or compound is provided with 5 series concentrations, each concentration is provided with 3 times of repeated treatment, and bread crumbs without medicine are set as a blank control test.
4. Data processing
Bioassay results LC was calculated using the Probit program analysis of SPSS 20.0 software50And calculating the CTC of the combined virulence of the two agents. The results are shown in table 2:
TABLE 2 Combined virulence of propoxur and acetamiprid against Periplaneta americana
As can be seen from the data in Table 2, when the mass ratio of propoxur to acetamiprid is 10:1-1:10, the CTC of the periplaneta americana is all over 120, and the synergistic effect is achieved. Especially, when the mass ratio of the CTC to the CTC is 1:1-1:4, the CTC has stronger synergistic effect at about 200.
Example 3 Dermatocet and acetamiprid on Solenopsis invicta (A)Monomorium pharaonis) Combined virulence determination of
1. Preparation of poison bait
Weighing propoxur (95% TC) and acetamiprid (96% TC) according to different mass ratios to prepare 0.1% acetone mother solution, diluting the acetone mother solution into diluents with series concentrations, adding the diluents with different concentrations into artificial feed (bread crumbs), uniformly stirring, and naturally volatilizing the acetone to obtain the test bait.
2. Test apparatus
Enamel square dish (white, 40cm x 30cm x 40 cm).
3. Test procedure
According to the national standard, part 7 of pesticide laboratory efficacy test and evaluation of sanitary insecticides for pesticide registration: relevant requirements of bait (GB/T13917.7-2009) tests were carried out, all in a space where the temperature and humidity were relatively stable [ temperature: (26 ± 1) ° c, relative humidity: 60% ± 10% ] is carried out. The test adopts an enamel square plate, a circle of Vaseline belt is coated on the edge of the inner wall of the opening of the enamel square plate, 100 little yellow ant workers over 3 days are put in the enamel square plate, after the workers return to normal activities, test bait is placed in the center of the enamel square plate, the death condition of the test insects is observed day by day, the dead insects are taken out in time, the death number of the test insects is recorded continuously until 7 days after feeding, and the death rate is calculated. Each medicament or compound is provided with 5 series concentrations, each concentration is provided with three times of repeated treatment, and a blank control test is set.
4. Data processing
The result is analyzed and calculated by the Probit program of SPSS 20.0 software to obtain LC50And calculating the CTC of the combined virulence of the two agents. The results are shown in Table 3:
TABLE 3 Combined virulence of propoxur and acetamiprid against yellow ants
As can be seen from the data in Table 3, when the mass ratio of propoxur to acetamiprid is 10:1-1:10, the CTC of the ant is all over 120, and the synergistic effect is achieved. Particularly, when the mass ratio of the CTC to the CTC is 2:1-1:4, the CTC has more than 200, and the synergistic effect is obvious.
EXAMPLE 4 preparation of bait matrix
1. The bait is prepared according to the following steps:
(1) weighing 4g of carbomer-940, adding 400mL of water for dissolving, then adding 8mL of 10% NaOH, and stirring to obtain gel carbomer;
(2) weighing 240g of sorbitol, 20g of cream essence and 10g of sodium benzoate, adding 28g of isopropanol and 24g of ethanol, uniformly mixing, adding the carbomer gel obtained in the step (1), and mixing and stirring until the carbomer gel is completely dissolved;
(3) weighing 5G of sodium alginate (350-410 mPa.s, M/G = 3), adding the sodium alginate into 40G of water, heating to 80 ℃, stirring until the sodium alginate is dissolved, slowly dropwise adding 10mL of 5% calcium chloride solution, keeping the temperature and quickly stirring to obtain a mixed solution;
(4) and (3) adding the mixed solution obtained in the step (3) into the gel obtained in the step (2) and uniformly stirring to obtain the gel bait matrix S1.
And (3) weighing different components according to the raw material proportion in the steps (1) to (4), and preparing the gel bait matrix S2 according to the method, wherein the difference is that the M/G value of the adopted sodium alginate is 1.2.
The gel bait matrix S3 was prepared according to the raw material ratio and method described in steps (1) to (4), except that the calcium chloride solution in step (3) had a concentration of 10% and the amount added was 20 mL.
2. Extrusion characteristics
The matrix is divided into 5mL needle cylinders, 5g of the matrix is placed in each needle cylinder, after standing for 24h, a needle cylinder piston is pushed to push out a certain amount of matrix, and the state of the matrix is observed: s1 is a uniform spherical gel; the matrix of S2 is hard and brittle and is crushed by extrusion; the matrix of S3 is difficult to squeeze and coagulates into relatively complete jelly-like gel in the syringe. This shows that only the proper M/G value of sodium alginate and the proper mass ratio of sodium alginate to calcium chloride can make the matrix have better viscosity and hardness and be suitable for extrusion products.
3. Water retention property
The substrate was divided into trapping boxes, each box was filled with 2g of the substrate, the trapping boxes were placed in an environment of 25 ℃ and 50% relative humidity, and the weight loss and hardening conditions were observed, and the results of the weight loss rate of 5 boxes for each sample are shown in table 4:
TABLE 4 comparison of weight loss ratios for different substrates (mean. + -. standard deviation)
Different letters in the same column represent p <0.05 (Duncan test).
As can be seen from the data in Table 4, the water loss rate of S2 is higher than that of the other two samples after being placed for 30 days, and the weight loss rates of the three samples at the other observation points are not significantly different. At the end of the test, however, samples S2 and S3 were dry and hard, making it difficult to separate the pieces with forceps and no longer suitable for the pest to eat; while sample S1 was soft and still able to be grasped with forceps, indicating that it could be eaten by pests. Therefore, the substrate formula disclosed by the invention not only has good water retention property, but also can keep the palatability for a long time, and is suitable for pests to eat.
EXAMPLE 5 preparation of gel bait
Weighing 5.21g 96% acetamiprid technical material and 10.53g 95% propoxur technical material, dissolving with a small amount of acetone, adding 10% tween-80 solution to prepare 200g of emulsion, adding 800g of the matrix S1 obtained in the step example 4, stirring at high speed for 30min, and standing to obtain 1.5% propoxur acetamiprid (1: 2) gel bait. 2% of propoxur acetamiprid (1: 2) gel bait, 0.03% of propoxur acetamiprid (1: 1) gel bait and 5% of propoxur acetamiprid (1: 4) gel bait were prepared according to the above steps.
2.5g of gel bait is weighed and placed in an environment with 25 ℃ and 50% relative humidity, the water loss and hardening conditions are observed, when the test is finished 90 days after the test, the gel bait has no mildew and complete hardening phenomena, and no medicament precipitation and the like exists in 0.03-5% of effective components, which indicates that the prepared gel bait is qualified.
Example 6 in-situ experiment of propoxur acetamiprid gel bait
1. Housefly
The on-site pesticide effect evaluation of the bait agent on the housefly is carried out according to WS/T576-: 4 catering units with similar housefly density are selected as test sites, the insecticide is not used for killing the housefly within one week before the test, and the housefly density is more than 10 flies/m2Wherein 2 users are test groups, 1 user is a control agent group (1.5% propoxur fly killing bait with registration number WP 20170005), and 1 user is a blank control group, and the test groups are repeated for 3 times on different sites. According to 5g/10m2The dosage of the preparation is as followsThe fly killing bait is placed in a culture dish every 10m2And arranging a group of the drug delivery devices at equal intervals on a test site, and applying no drug to a control area. Measuring the field fly density after the bait is distributed for 4h and 24h, and calculating the relative density reduction rate (%):
Rd=（1- 
）×100（%）
in the formula:R d —a relative density decrease rate;
T a -average density value after application area treatment;
T b -pre-treatment average density value of the application area;
C a -average density value after blank control area treatment;
C b -average density value before treatment of blank control area.
TABLE 51.5% of the insecticide Weiwei-acetamiprid bait for killing houseflies on-site experiment
According to the WS/T576-2017 standard, the relative density reduction rate of the houseflies is more than 75% after the two medicaments are applied for 4 hours, the relative density reduction rate of the houseflies is more than 85% after the two medicaments are applied for 24 hours, and the fly killing effect is qualified. According to the data in the table 5, the killing effect of the 1.5% propoxur acetamiprid gel bait on the houseflies after 4 hours and 24 hours after the pesticide is applied is obviously better than that of a control medicament.
2. Blatta Seu Periplaneta
Reference GB/T27783-: 4 catering units with serious cockroach invasion and similar density are selected as test sites, and the areas are all about 100m2German XiaoThe density of the cockroach is more than 10 cockroaches per cockroach, wherein 2 users are a test group, 1 user is a control agent group (2.15% imidacloprid cockroach killing gel bait with registration number WP 20080052), 1 user is a blank control group, the cockroach killing gel bait is uniformly distributed on breeding and inhabiting places of the cockroaches, and the dosage of the preparation of the test group is 0.1g/m2The dosage of the control agent group preparation is 0.3g/m2The cockroaches density in the test area and the blank control area were measured 3 days, 7 days, 14 days and 30 days after the application of the drug without the drug in the blank control area, and the relative density decrease rate (%) was calculated according to the following formula:
Rd=（1- 
）×100（%）
in the formula:R d —a relative density decrease rate;
T a -average density value after application area treatment;
T b -pre-treatment average density value of the application area;
C a -average density value after blank control area treatment;
C b -average density value before treatment of blank control area.
On-site experiment of killing cockroach with 62% of propoxur acetamiprid gel bait
Referring to the GB/T27783-. As is clear from the data in Table 6, the killing effect of 2% propoxur acetamiprid gel bait prepared by the invention on cockroach was significantly better than that of the control agent in 3 days, 7 days, 14 days and 30 days after the application.
3. Ant of yellow colour
Choose to have small4 residents of the residences endangered by the yellow ants are used as test sites. Wherein 2 users are test groups, 1 user is a control agent group (0.03% imidacloprid termite-killing gel bait with registration number WP 20120237), and 1 user is a blank control group. The dosage of the test group preparation is 0.1g/m2The dosage of the control agent group preparation is 0.2g/m2In the control area, no medicine is used, 3 observation points are selected on the ant path 1 day before the poison bait is distributed and 3 days, 7 days and 14 days after the poison bait is distributed, a small amount of biscuits are distributed on the observation points to serve as baits, the number of ants on the baits is observed after the baits are distributed for 30min and serves as a density index, and the relative density reduction rate (%):
Rd=（1- 
）×100（%）
in the formula:R d —a relative density decrease rate;
T a -average density value after application area treatment;
T b -pre-treatment average density value of the application area;
C a -average density value after blank control area treatment;
C b -average density value before treatment of blank control area.
Site experiment of killing ant with pesticide residue No. 2 in table 70.03%
As can be seen from the data in Table 7, the relative density reduction rate of the small yellow ants is more than 85% and the effect is remarkable 7 days after the two medicaments are applied. The control medicament has reduced control effect after 14 days of administration, and needs to be administered again to maintain better killing effect. The killing effect of the 0.03% propoxur acetamiprid gel bait on the yellow ants is always high after 3 days, 7 days and 14 days of pesticide application, and the pesticide can be kept for at least 14 days without re-dosing.
According to the data in tables 5-7, the sanitary insecticidal composition can effectively kill houseflies, cockroaches and ants, effectively reduce the density of pests, and has the advantages of low dosage and long lasting period.
Claims (10)
1. A sanitary insecticidal composition is characterized in that the effective components are propoxur and acetamiprid; the mass ratio of the propoxur to the acetamiprid is 10:1-1: 10.
2. A sanitary insecticidal composition as claimed in claim 1, wherein the mass ratio of propoxur to acetamiprid is 2:1-1: 4.
3. A sanitary insecticidal composition according to claim 1 wherein said active ingredient is present in an amount of 0.01 to 5% by weight of the composition.
4. A sanitary insecticidal composition according to claim 1 wherein said active ingredient is present in the composition in an amount of 0.5 to 2% by weight.
5. The sanitary insecticide composition according to claim 1, wherein the formulation of the sanitary insecticide composition is selected from any one of a propellant, an aerosol, a bait or a powder.
6. The sanitary insecticide composition according to claim 1, wherein the formulation of the sanitary insecticide composition is bait.
7. A sanitary insecticidal composition according to any one of claims 1 to 6 wherein the control objects of said sanitary insecticidal composition are ants, cockroaches and flies.
8. The bait matrix for the sanitary insecticide is characterized by comprising the following raw materials in parts by weight: 430 parts of carbomer gel, 5-15 parts of sodium alginate, 1.4-2 parts of calcium chloride, 250 parts of sorbitol, 15-20 parts of attractant, 1-10 parts of preservative, 25-30 parts of isopropanol, 20-30 parts of ethanol and 50-70 parts of water;
the viscosity of the sodium alginate is 300-500mPa.s, and the M/G ratio is 2.5-3.2;
the mass ratio of the sodium alginate to the calcium chloride is 10: 1-1.4.
9. The bait matrix of claim 8, wherein carbomer type 940 or 941; the concentration of the carbomer gel is 0.5-2%.
10. The bait matrix of claim 8, wherein the attractant is selected from the group consisting of sugars, animal and vegetable fats and oils, or flavors; the preservative is a bactericide or an antioxidant for resisting fungi and bacteria.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1265269A (en) * | 1999-09-21 | 2000-09-06 | 马越 | Bendiocarb-fenpropathrin pesticide composition |
| CN1457637A (en) * | 2002-05-16 | 2003-11-26 | 李晔 | Contact killing type insecticide paste |
| CN109221227A (en) * | 2018-10-21 | 2019-01-18 | 江苏长青生物科技有限公司 | It is a kind of efficiently to kill cockroach gel bait formulation and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1265269A (en) * | 1999-09-21 | 2000-09-06 | 马越 | Bendiocarb-fenpropathrin pesticide composition |
| CN1457637A (en) * | 2002-05-16 | 2003-11-26 | 李晔 | Contact killing type insecticide paste |
| CN109221227A (en) * | 2018-10-21 | 2019-01-18 | 江苏长青生物科技有限公司 | It is a kind of efficiently to kill cockroach gel bait formulation and preparation method thereof |
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| Title |
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| "开发海藻酸水凝胶饵剂管理蚂蚁";罗梁锋;《世界农药》;20171231;第39卷(第6期);第43页 * |
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