CN110437988A - A kind of extract separating pipe and its application method - Google Patents
A kind of extract separating pipe and its application method Download PDFInfo
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- CN110437988A CN110437988A CN201910693110.3A CN201910693110A CN110437988A CN 110437988 A CN110437988 A CN 110437988A CN 201910693110 A CN201910693110 A CN 201910693110A CN 110437988 A CN110437988 A CN 110437988A
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- tube
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000000605 extraction Methods 0.000 claims abstract description 7
- 239000008267 milk Substances 0.000 claims abstract description 5
- 210000004080 milk Anatomy 0.000 claims abstract description 5
- 235000013336 milk Nutrition 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims description 17
- 239000004743 Polypropylene Substances 0.000 claims description 3
- -1 polypropylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 abstract description 6
- 102000039446 nucleic acids Human genes 0.000 abstract description 6
- 108020004707 nucleic acids Proteins 0.000 abstract description 6
- 238000000703 high-speed centrifugation Methods 0.000 abstract description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 208000004396 mastitis Diseases 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
技术领域technical field
本发明涉及试管技术领域，特别涉及一种无菌奶牛乳房炎核酸提取物分离管及其使用方法。The invention relates to the technical field of test tubes, in particular to a sterile cow mastitis nucleic acid extraction tube and a method for using the same.
背景技术Background technique
在奶牛乳房炎核酸提取过程中，需要将牛奶中的脂肪除去。常规的方法是使用高速离心法使得核酸提取物与脂肪分层(高速离心后核酸提取物沉淀在管底，脂肪等在上层，用棉签将上层脂肪擦去。若棉签力度不当容易将下层沉淀物擦去，并且这一过程较为耗时繁琐。During the nucleic acid extraction process of cow mastitis, the fat in the milk needs to be removed. The conventional method is to use high-speed centrifugation to separate the nucleic acid extract and fat (the nucleic acid extract precipitates at the bottom of the tube after high-speed centrifugation, and the fat is on the upper layer. Use a cotton swab to wipe off the upper layer of fat. If the cotton swab is not strong enough, it is easy to remove the lower layer of sediment Erase, and this process is time-consuming and cumbersome.
发明内容Contents of the invention
本发明针对现有技术的缺陷，提供了一种提取物分离管及其使用方法，能有效的解决上述现有技术存在的问题。Aiming at the defects of the prior art, the present invention provides an extract separation tube and its use method, which can effectively solve the above-mentioned problems in the prior art.
为了实现以上发明目的，本发明采取的技术方案如下：In order to realize above object of the invention, the technical scheme that the present invention takes is as follows:
一种提取物分离管，包括：上管1、下管2和上盖3，所述上管1为圆管状且上下开口连通；下管2为上部开口的下部封口的圆管状；An extract separation tube, comprising: an upper tube 1, a lower tube 2 and an upper cover 3, the upper tube 1 is in the shape of a round tube and the upper and lower openings are connected; the lower tube 2 is in the shape of a round tube with an upper opening and a lower seal;
上管1的下开口内壁设有螺纹，下管2的上部开口外壁设有螺纹接口，上管1和下管2通过螺纹与螺纹接口螺纹连接；The inner wall of the lower opening of the upper pipe 1 is provided with threads, and the outer wall of the upper opening of the lower pipe 2 is provided with a threaded interface, and the upper pipe 1 and the lower pipe 2 are threadedly connected to the threaded interface through threads;
上管1的上开口设有上盖3，用于盖住上开口。The upper opening of the upper tube 1 is provided with an upper cover 3 for covering the upper opening.
进一步地，所述上管1、下管2和上盖3材料均为聚丙烯。Further, the materials of the upper tube 1, the lower tube 2 and the upper cover 3 are all polypropylene.
上述分离管使用方法如下：The method of using the above separation tube is as follows:
(1)将上管1与下管2从无菌包装中取出，通过螺纹无缝连接；(1) Take the upper tube 1 and the lower tube 2 out of the aseptic package, and connect them seamlessly through threads;
(2)取1ml待检测的牛奶从上管1注入，然后盖上上盖3，将分离管置于高速离心机12000r/min离心5min；(2) Take 1ml of milk to be tested and inject it from the upper tube 1, then cover the upper cover 3, and place the separation tube in a high-speed centrifuge at 12000r/min for 5min;
(3)打开上盖3使用移液枪头或者其他工具将上层脂肪层打散(以免步骤4倒置后液体流出)，后盖上盖；(3) Open the upper cover 3 and use a pipette tip or other tools to break up the upper fat layer (to prevent the liquid from flowing out after step 4 is inverted), and then cover the upper cover;
(4)将分离管倒置，一手固定上管1另一手旋动下管2，使得下管2与上管1分离，丢弃上管1；(4) Turn the separation tube upside down, fix the upper tube 1 with one hand and rotate the lower tube 2 with the other hand, so that the lower tube 2 is separated from the upper tube 1, and discard the upper tube 1;
(5)将下管2与新的上管1通过螺纹接口连接完成后续的提取步骤。(5) Connect the lower tube 2 with the new upper tube 1 through a threaded interface to complete the subsequent extraction steps.
与现有技术相比本发明的优点在于：使用一次性两段式管身结构能够有效地简化脂肪清除过程提高核酸提取效率。Compared with the prior art, the present invention has the advantages that: the use of a disposable two-section tube body structure can effectively simplify the fat removal process and improve the efficiency of nucleic acid extraction.
附图说明Description of drawings
图1为本发明实施例分离管的结构示意图；Fig. 1 is the structural representation of separation tube of the embodiment of the present invention;
图2为本发明实施例分离管分离后的结构示意图。Fig. 2 is a schematic diagram of the structure of the separation tube of the embodiment of the present invention after separation.
具体实施方式Detailed ways
为使本发明的目的、技术方案及优点更加清楚明白，以下结合附图并列举实施例，对本发明做进一步详细说明。In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and examples.
如图1、2所示，一种提取物分离管，包括上管1、下管2和上盖3，所述上管1为圆管状且上下开口连通；下管2为上部开口的下部封口的圆管状；As shown in Figures 1 and 2, an extract separation tube includes an upper tube 1, a lower tube 2 and an upper cover 3, the upper tube 1 is in the shape of a round tube and the upper and lower openings are connected; the lower tube 2 is the lower seal of the upper opening round tubular shape;
上管1的下开口内壁设有螺纹，下管2的上部开口外壁设有螺纹接口，上管1和下管2通过螺纹与螺纹接口螺纹连接；The inner wall of the lower opening of the upper pipe 1 is provided with threads, and the outer wall of the upper opening of the lower pipe 2 is provided with a threaded interface, and the upper pipe 1 and the lower pipe 2 are threadedly connected to the threaded interface through threads;
上管1的上开口设有上盖3，用于盖住上开口。The upper opening of the upper tube 1 is provided with an upper cover 3 for covering the upper opening.
所述上管1、下管2和上盖3材料为聚丙烯。The upper tube 1, the lower tube 2 and the upper cover 3 are made of polypropylene.
上述分离管使用方法如下：The method of using the above separation tube is as follows:
(1)将上管1与下管2从无菌包装中取出，通过螺纹无缝连接；(1) Take the upper tube 1 and the lower tube 2 out of the aseptic package, and connect them seamlessly through threads;
(2)取1ml待检测的牛奶从上管1注入，然后盖上上盖3，将分离管置于高速离心机12000r/min离心5min；(2) Take 1ml of milk to be tested and inject it from the upper tube 1, then cover the upper cover 3, and place the separation tube in a high-speed centrifuge at 12000r/min for 5min;
(3)打开上盖3使用移液枪头或者其他工具将上层脂肪层打散(以免步骤4倒置后液体流出)，后盖上盖；(3) Open the upper cover 3 and use a pipette tip or other tools to break up the upper fat layer (to prevent the liquid from flowing out after step 4 is inverted), and then cover the upper cover;
(4)将分离管倒置，一手固定上管1另一手旋动下管2，使得下管2与上管1分离，丢弃上管1；(4) Turn the separation tube upside down, fix the upper tube 1 with one hand and rotate the lower tube 2 with the other hand, so that the lower tube 2 is separated from the upper tube 1, and discard the upper tube 1;
(5)将下管2与新的上管1通过螺纹接口连接完成后续的提取步骤。(5) Connect the lower tube 2 with the new upper tube 1 through a threaded interface to complete the subsequent extraction steps.
本领域的普通技术人员将会意识到，这里所述的实施例是为了帮助读者理解本发明的实施方法，应被理解为本发明的保护范围并不局限于这样的特别陈述和实施例。本领域的普通技术人员可以根据本发明公开的这些技术启示做出各种不脱离本发明实质的其它各种具体变形和组合，这些变形和组合仍然在本发明的保护范围内。Those skilled in the art will appreciate that the embodiments described here are to help readers understand the implementation method of the present invention, and it should be understood that the protection scope of the present invention is not limited to such specific statements and embodiments. Those skilled in the art can make various other specific modifications and combinations based on the technical revelations disclosed in the present invention without departing from the essence of the present invention, and these modifications and combinations are still within the protection scope of the present invention.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910693110.3A CN110437988A (en) | 2019-07-30 | 2019-07-30 | A kind of extract separating pipe and its application method |
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| CN201910693110.3A CN110437988A (en) | 2019-07-30 | 2019-07-30 | A kind of extract separating pipe and its application method |
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| CN110437988A true CN110437988A (en) | 2019-11-12 |
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Application publication date: 20191112 |