CN110423746B - Gene sequence encoding small peptide PFER, small peptide PFER and application and method for inducing plant rooting - Google Patents
Gene sequence encoding small peptide PFER, small peptide PFER and application and method for inducing plant rooting Download PDFInfo
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- CN110423746B CN110423746B CN201910639857.0A CN201910639857A CN110423746B CN 110423746 B CN110423746 B CN 110423746B CN 201910639857 A CN201910639857 A CN 201910639857A CN 110423746 B CN110423746 B CN 110423746B
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 23
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 20
- 230000001939 inductive effect Effects 0.000 title claims abstract description 17
- 241000196324 Embryophyta Species 0.000 claims description 37
- 241000219000 Populus Species 0.000 claims description 14
- 239000012224 working solution Substances 0.000 claims description 12
- 238000002791 soaking Methods 0.000 claims description 9
- 239000002689 soil Substances 0.000 claims description 9
- 150000007523 nucleic acids Chemical group 0.000 claims description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 239000012636 effector Substances 0.000 abstract 1
- 241001149649 Taxus wallichiana var. chinensis Species 0.000 description 9
- 241000249899 Populus tomentosa Species 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 241001116500 Taxus Species 0.000 description 4
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 4
- 241001278112 Populus euphratica Species 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000012452 mother liquor Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 230000002194 synthesizing effect Effects 0.000 description 3
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 2
- 235000010928 Betula populifolia Nutrition 0.000 description 2
- 244000089654 Betula populifolia Species 0.000 description 2
- 241000202349 Taxus brevifolia Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000003617 indole-3-acetic acid Substances 0.000 description 2
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 235000006667 Aleurites moluccana Nutrition 0.000 description 1
- DPNWSMBUYCLEDG-CIUDSAMLSA-N Asp-Lys-Ser Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O DPNWSMBUYCLEDG-CIUDSAMLSA-N 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 235000009113 Betula papyrifera Nutrition 0.000 description 1
- 235000009109 Betula pendula Nutrition 0.000 description 1
- 235000002992 Betula pubescens Nutrition 0.000 description 1
- 240000004957 Castanea mollissima Species 0.000 description 1
- 235000018244 Castanea mollissima Nutrition 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 241001489978 Eupolyphaga Species 0.000 description 1
- 244000141359 Malus pumila Species 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 240000004923 Populus tremuloides Species 0.000 description 1
- 235000011263 Populus tremuloides Nutrition 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 240000001987 Pyrus communis Species 0.000 description 1
- HQTKVSCNCDLXSX-BQBZGAKWSA-N Ser-Arg-Gly Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(O)=O HQTKVSCNCDLXSX-BQBZGAKWSA-N 0.000 description 1
- 244000162450 Taxus cuspidata Species 0.000 description 1
- 235000009065 Taxus cuspidata Nutrition 0.000 description 1
- 208000026487 Triploidy Diseases 0.000 description 1
- 108010072041 arginyl-glycyl-aspartic acid Proteins 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000550 effect on aging Effects 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Wood Science & Technology (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Agronomy & Crop Science (AREA)
- Developmental Biology & Embryology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention belongs to the technical field of plant molecular biology, and provides a gene sequence for coding a small peptide PFER (polypeptide-like effector) and the small peptide PFER, as well as application and a method for inducing plant rooting, wherein the gene sequence is shown as SEQ ID NO. 1, in order to promote plant rooting. The method can effectively promote the rooting of the forest plants.
Description
Technical Field
The invention relates to the technical field of plant molecular biology, in particular to a gene sequence for coding a small peptide PFER, the small peptide PFER, application and a method for inducing plant rooting.
Background
The description of the background of the invention pertaining to the related art to which this invention pertains is given for the purpose of illustration and understanding only of the summary of the invention and is not to be construed as an admission that the applicant is explicitly or implicitly admitted to be prior art to the date of filing this application as first filed with this invention.
The ability of a plant to root, also known as the root's ability to recur, is closely linked to its survival rate. The stem cutting rooting is generally to form a root original body on a cambium and a vascular bundle sheath tissue of a base stem, so that an adventitious root grows out, a root system is formed, nutrition and moisture are absorbed, and the survival of a plant is ensured. However, the rooting ability of the aged trees is affected by factors such as increase of secondary metabolites, weakening of cambium stem cell activity and the like, so that the regeneration ability of roots is obviously reduced. Traditional plant growth hormones, such as auxins IAA (indoleacetic acid), IBA (indolebutyric acid), NAA (naphthylacetic acid) and the like, only have limited capability of promoting rooting of forest seedlings or adult seedlings, but have poor effect on aging trees.
How to promote the rooting capacity of woody plants is a problem to be solved urgently at present.
Disclosure of Invention
The embodiment of the invention aims to provide a gene sequence for coding a small peptide PFER, the small peptide PFER, application and a method for inducing plant rooting.
The purpose of the embodiment of the invention is realized by the following technical scheme:
the embodiment of the first aspect of the invention provides a gene sequence for coding a small peptide PFER, and the gene sequence is shown as SEQ ID NO. 1.
An embodiment of the second aspect of the present invention provides a small peptide PFER comprising a base sequence shown in SEQ ID NO. 2.
Furthermore, the small peptide PFER is obtained by coding a gene sequence shown as SEQ ID NO. 1.
The embodiment of the third aspect of the invention provides an application of a small peptide PFER in forest plants, wherein the application is to induce plants to root and/or promote cuttage to root, and the small peptide PFER comprises a base sequence shown as SEQ ID NO. 2.
Further, the plant is poplar or taxus chinensis.
The embodiment of the fourth aspect of the invention provides a method for inducing plant rooting by using a small peptide PFER, which comprises the step of soaking forest plants by using a working solution containing the small peptide PFER, wherein the nucleic acid sequence of the small peptide is shown as SEQ ID NO. 1, the concentration of the small peptide PFER in the working solution is 1-10 mu M, and the soaking time is 24-72 hours.
Further, the method comprises the following steps:
(1) artificially synthesizing small peptide PFER, preparing into aqueous solution, and diluting into working solution with concentration of 1-10 μ M;
(2) cutting a wound with the length of 0.5-2cm at the base of the old tree branch, and soaking for 24-72 h;
(3) and re-inserting the plant into soil for cultivation, and inducing rooting.
Further, the working solution was used at a concentration of 1. mu.M.
Further, the treatment time was 24 hours.
Further, the plants are poplar and yew.
Compared with the prior art, the gene sequence for coding the small peptide PFER, the application and the method for inducing the plant to root have at least the following beneficial effects:
the invention adopts the exogenous small peptide PFER, and the exogenous application of the small peptide can obviously promote the plant to take root, thereby having important significance for improving the survival rate of poplar planting and improving the efficiency of artificial forest cultivation.
Drawings
FIG. 1 is a gene sequence of the coding small peptide PFER, the application and the method for inducing the plant to root, the base sequence of the small peptide and the effect picture of the Chinese white poplar branch which has rooted in 25 days;
FIG. 2 is a 7-day effect diagram of a populus tremuloides branch with heavy roots by using the gene sequence for coding the small peptide PFER, the small peptide PFER and the method for applying and inducing plants to root;
FIG. 3 is a gene sequence of the small peptide PFER, the small peptide PFER and the effect diagram of the small peptide treated rooting Chinese yew branch;
FIG. 4 is a gene sequence of the coding small peptide PFER, the small peptide PFER and the application and method for inducing plant rooting, and the effect picture of the treated rooted populus diversifolia branches with the small peptide.
Detailed Description
The present application is further described with reference to the following figures and examples.
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following will describe specific embodiments of the present invention. In the following description, different "one embodiment" or "an embodiment" refers to not necessarily the same embodiment. In addition, the following describes only some embodiments of the present invention, and different embodiments may be replaced or combined, and other embodiments may be implemented by those skilled in the art without inventive effort.
A gene sequence for coding a small peptide PFER is shown as SEQ ID NO. 1.
A small peptide PFER, said small peptide PFER comprising a base sequence as set forth in SEQ ID NO. 2.
In some embodiments of the invention, the small peptide PFER is encoded by the gene sequence shown in SEQ ID NO. 1.
The application of the small peptide PFER in forest plants is to induce plants to root and/or promote cuttage to root, and the small peptide PFER comprises a base sequence shown as SEQ ID NO. 2.
In some embodiments of the invention, the plant is poplar or taxus chinensis.
A method for inducing plants to root by small peptide PFER comprises the steps of soaking forest plants by adopting working solution containing the small peptide PFER, wherein the nucleic acid sequence of the small peptide is shown as SEQ ID NO. 1, the concentration of the small peptide PFER in the working solution is 1-10 mu M, and the soaking time is 24-72 hours.
In some embodiments of the invention, the method comprises the following steps:
(1) artificially synthesizing small peptide PFER, preparing into aqueous solution, and diluting into working solution with concentration of 1-10 μ M;
(2) cutting a wound with the length of 0.5-2cm at the base of the old tree branch, and soaking for 24-72 h;
(3) and re-inserting the plant into soil for cultivation, and inducing rooting.
In some embodiments of the invention, the working fluid is used at a concentration of 1 μ M.
In some embodiments of the invention, the time processed is 24 h.
In some embodiments of the invention, the plants are poplar and yew.
The sequence of the small peptide is as follows: LSRGDKSEL
Leucine-serine-arginine-glycine-aspartic acid-lysine-serine-glutamic acid-leucine. At least comprises the following steps: SRGDKS, namely: -serine-arginine-glycine-aspartic acid-lysine-serine-.
The technical scheme is as follows: (1) taking a triploid Chinese white poplar plant (Populus tomentosa) with the age of 30 years, grown in a campus of Beijing forestry university, and a plurality of branches of adult Taxus brevifolia (Taxus baccata Ericoides) and Populus diversifolia (Populus eupolyphaga);
(2) directly synthesizing PFER small peptide by Shanghai bioengineering limited company according to nucleic acid and base sequence, adding water in proportion, dissolving into 1mM mother solution, diluting with water 1000-fold and 100-fold for use, and the final concentration is 1-10 μ M; (3) soaking poplar and yew branches in diluted small molecular peptide and water solution for 24 hr, and culturing in artificial climate chamber; (4) after 1 month (poplar) and 2 months (yew), the shoots were carefully removed for 1 month (populus euphratica) for rooting statistics and photographed.
The specific implementation mode is that the small peptide is synthesized by Shanghai bioengineering limited company, 1mM mother liquor is prepared, water is added for dilution to prepare treatment fluid with the final concentration of 1 mu M, populus tomentosa branches and taxus chinensis branches which grow in the field and are 30 years old are taken, the populus tomentosa branches and the taxus chinensis branches are cut into small branches with the same size and growth vigor, then the small branches are placed into the treatment fluid for soaking for 24 hours, the small branches are moved into soil with the volume ratio of three components of turfy soil, nutrient soil and vermiculite being 1:1:1, and the branch rooting condition is observed and counted in a climatic chamber for about 1 month.
The first embodiment is as follows:
the amino acid coded by the nucleic acid sequence is directly synthesized into small peptide PFER by Shanghai bioengineering (Shanghai) GmbH, the synthetic amount is 10mg, the synthetic product is white powder, the white powder is diluted by distilled water to prepare mother liquor with the concentration of 1mM, and then the mother liquor is diluted by 1000 times to prepare working solution.
Example two:
the method comprises the steps of taking a Chinese white poplar plant growing in the field and having the age of 30 years and a Taxus brevifolia plant branch which are identified by an annual ring method, keeping terminal buds, cutting the branches into small branches with the same growth vigor and the length of about 12cm, and cutting a wound at the position of 0.5cm at the bottom of the branch by using a blade.
Example three:
prepared poplar and taxus chinensis branches were soaked in PFER small peptide aqueous solution of final concentration of 1 μ M as a treatment group, and the control group was soaked with water for 24 hours with a repetition number of 20.
Example four:
and (3) transplanting the soaked poplar and taxus chinensis twigs into soil with the ratio of turfy soil to nutrient soil to vermiculite being 1:1:1, and observing and counting the rooting condition of the twigs in an artificial climate room for 1 month and 2 months or so.
The results show that after poplar and taxus chinensis branches are treated by small peptide PFER with 9 amino acids for 24 hours, the branches can be induced to generate a large number of roots at the roots, a technology for rapidly inducing forest plants to root is provided, and the method can promote the artificial propagation of important afforestation tree species populus tomentosa and the rare or endangered plant taxus chinensis. The method can be used for other forest tree species or shrub plants which are difficult to root, and the woody plants such as poplar, pine, cypress, fir and white birch or the economic forest woody plants such as apple, pear, Chinese chestnut, orange and the like are used for rapidly inducing the root, thereby having important significance for promoting the industrialization and rapid propagation of the woody plants and large-area artificial planting.
It should be noted that the above embodiments can be freely combined as necessary. The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Sequence listing
<110> Beijing university of forestry
<120> gene sequence for coding small peptide PFER, application and method for inducing plant rooting
<130> 1
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 27
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
ctgagccgcg gcgataaaag cgaactg 27
<210> 2
<211> 6
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 2
Ser Arg Gly Asp Lys Ser
1 5
Claims (8)
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| CN201910639857.0A CN110423746B (en) | 2019-07-16 | 2019-07-16 | Gene sequence encoding small peptide PFER, small peptide PFER and application and method for inducing plant rooting |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910639857.0A CN110423746B (en) | 2019-07-16 | 2019-07-16 | Gene sequence encoding small peptide PFER, small peptide PFER and application and method for inducing plant rooting |
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| Publication Number | Publication Date |
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| CN110423746B true CN110423746B (en) | 2021-03-12 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101815784A (en) * | 2006-02-13 | 2010-08-25 | 美国弗劳恩霍夫股份有限公司 | Production of foreign nucleic acids and polypeptides in plant systems |
| WO2013104026A1 (en) * | 2012-01-11 | 2013-07-18 | The Australian National University | Method for modulating plant root architecture |
| CN107467074A (en) * | 2017-06-26 | 2017-12-15 | 浦江县昂宝生物技术有限公司 | Growth promotion plant growth regulator |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103554236B (en) * | 2013-11-05 | 2015-06-17 | 李�昊 | Short peptide for promoting plants to take roots, sprout, grow and prolong storage period and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101815784A (en) * | 2006-02-13 | 2010-08-25 | 美国弗劳恩霍夫股份有限公司 | Production of foreign nucleic acids and polypeptides in plant systems |
| WO2013104026A1 (en) * | 2012-01-11 | 2013-07-18 | The Australian National University | Method for modulating plant root architecture |
| CN107467074A (en) * | 2017-06-26 | 2017-12-15 | 浦江县昂宝生物技术有限公司 | Growth promotion plant growth regulator |
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