CN110404597B - Multifunctional self-cleaning pipetting device and application method thereof - Google Patents
Multifunctional self-cleaning pipetting device and application method thereof Download PDFInfo
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- CN110404597B CN110404597B CN201910728165.3A CN201910728165A CN110404597B CN 110404597 B CN110404597 B CN 110404597B CN 201910728165 A CN201910728165 A CN 201910728165A CN 110404597 B CN110404597 B CN 110404597B
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- liquid
- storage bin
- cleaning
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- pipette
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/56—Labware specially adapted for transferring fluids
- B01L3/561—Tubes; Conduits
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/093—Cleaning containers, e.g. tanks by the force of jets or sprays
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Cleaning By Liquid Or Steam (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a multifunctional self-cleaning pipetting device which comprises a liquid inlet pipeline, a liquid storage bin, a pipette, an adapter, a peristaltic pump and a pump pipe, wherein the bottom of the liquid storage bin is connected with the pipette, the top of the liquid storage bin is connected with the bottom of the adapter, a spray shower head is arranged in the liquid storage bin, the liquid inlet pipeline penetrates through the adapter and then is connected to the top of the spray shower head, two ends of the pump pipe are respectively connected to the top of the adapter and the peristaltic pump, and a clamping piece is inserted into one end, close to the liquid storage bin, of the pipette. In addition, the invention also discloses a using method of the multifunctional self-cleaning pipetting device. The peristaltic pump can be effectively prevented from being blocked, and the liquid including suspension, viscous liquid and clarified liquid can be transferred, stored and discharged; the automatic cleaning can be realized, the service life is prolonged, and the cost is reduced; the single treatment capacity is large, 150 milliliters of liquid can be transferred, the structure is simple, and the use is convenient.
Description
Technical Field
The invention relates to an automatic liquid transfer and related cleaning device, in particular to a multifunctional self-cleaning pipetting device. In addition, the invention also relates to a using method of the multifunctional self-cleaning pipetting device.
Background
The invention discloses a pipetting device and a pipetting method, wherein the pipetting device is used for sucking and filling a liquid sample through a disposable pipetting head, and in the descending process of a probe, the gas supply mechanism supplies gas to a gas channel pipe, so that the stability of the gas pressure in the gas channel pipe can be improved, when the pipetting head contacts with the liquid level of a body fluid sample, the gas pressure in the gas channel pipe is obvious relative to the gas pressure before the liquid level contacts with the body fluid sample, so that a worker can accurately judge the liquid level of the body fluid sample according to the gas pressure change in the gas channel pipe, and the accuracy of the liquid level detection of the body fluid sample is improved, and the pipetting device mainly solves the problem of the liquid level detection accuracy;
The invention provides a biomedical pipetting working platform with an automatic cell extraction function, which is disclosed in the patent with the patent application number of CN201910058715.5, and realizes automatic operation of a high-flux sample treatment process in an experimental operation flow;
The invention discloses liquid transfer equipment in the patent with the patent application number of CN201910058734.8, which can be used for conveying reagents, samples and the like into a plurality of holes on a porous plate, can freely select to carry out multi-channel liquid adding or single-channel liquid adding, and separate a liquid sucking fluid device from a liquid transferring device, so that a long enough stroke is ensured, and pollution is avoided;
However, in the industries of medicine, chemical industry and the like, the volume of each time of the three inventions is small, the maximum volume is not more than 2 milliliters, only clear liquid can be transferred, and the gun head is disposable and needs to be replaced after filling the liquid, so that the gun head can not be cleaned and regenerated automatically;
the invention discloses an automatic cleaning device for a liquid-transferring gun head, which can control the automatic cleaning device for the liquid-transferring gun head to execute an automatic control system of a preset cleaning program in a cleaning box, can effectively solve the cleaning problem of the liquid-transferring gun head, but is only suitable for cleaning the liquid-transferring gun head, the liquid storage volume of the cleaned gun head is not more than 5 milliliters at most, in addition, the invention is only one cleaning device, and can not solve the problem of liquid transfer,
In order to cooperate with an automatic use scene, unmanned 24-hour continuous operation is realized, meanwhile, the carrying of all types of liquid is realized, the reaction liquid is transferred, stored and discharged as required, each time of automatic online cleaning is not required to be opened manually, the existing products on the market cannot meet the application requirements, the suspension after the completion of the reaction can be transferred, and the single transfer quantity is more than 100 milliliters, so that a liquid-transferring cleaning device suitable for the automatic application scene needs to be designed and developed.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a multifunctional self-cleaning pipetting device which can effectively prevent a peristaltic pump from being blocked and realize the transfer, storage and discharge of liquid including suspension, viscous liquid and clarified liquid; the added liquid inlet pipeline and the spray shower can realize automatic cleaning, prolong the service lives of the liquid inlet pipeline, the liquid storage bin, the pipette and the adapter, and reduce the cost; the single treatment capacity is large, 150 milliliters of liquid can be transferred, the structure is simple, the use is convenient, and the defect caused by the prior art is overcome. Therefore, the invention also provides a using method of the multifunctional self-cleaning pipetting device.
The invention provides the following technical scheme for solving the technical problems:
The utility model provides a multi-functional self-cleaning pipetting device, wherein, includes feed liquor pipeline, stock solution storehouse, pipette, crossover sub, peristaltic pump, pump line, the bottom of stock solution storehouse with the pipette is connected, the top of stock solution storehouse with the bottom of crossover sub is connected, the inside of stock solution storehouse is equipped with sprays the gondola water faucet, the feed liquor pipeline run through behind the crossover sub connect in spray the top of gondola water faucet, the both ends of pump line connect respectively in the top of crossover sub with peristaltic pump, the pipette is near the one end of stock solution storehouse is inserted and is equipped with the chucking spare.
The multifunctional self-cleaning pipetting device comprises a liquid storage bin, wherein the top of the liquid storage bin is provided with a liquid inlet pipe connected with an adapter, the bottom of the adapter is provided with a first through hole communicated with the liquid inlet pipe, and the top of the adapter is provided with a second through hole communicated with a pump pipe.
The multifunctional self-cleaning pipetting device is characterized in that a liquid outlet pipe connected with the pipette is arranged at the bottom of the liquid storage bin.
The multifunctional self-cleaning pipetting device comprises a liquid storage bin, wherein the liquid storage bin comprises a cylindrical pipe body and an inverted cone pipe body which are sequentially and fixedly connected from top to bottom.
The multifunctional self-cleaning pipetting device is characterized in that the liquid inlet pipe is an inverted trapezoid liquid inlet pipe.
The multifunctional self-cleaning pipetting device is characterized in that the adapter is a glass frosted adapter.
The multifunctional self-cleaning pipetting device is characterized in that the clamping piece is a T-shaped clamping piece fixedly connected with the knob block through the insertion block, and a through hole for the insertion block to be inserted is formed in the pipette.
The multifunctional self-cleaning pipetting device is characterized in that the spraying shower head is a cylindrical spraying shower head, and a plurality of holes communicated with the liquid inlet pipeline are uniformly formed in the periphery of the cylindrical spraying shower head.
The multifunctional self-cleaning pipetting device comprises 50 holes, wherein the diameter of each hole is 0.7 mm.
In addition, the invention also provides a using method of the multifunctional self-cleaning pipetting device, which comprises a using method of transferring reaction mother liquor, a using method of rinsing a liquid storage bin and a related liquid flow path, and a using method of cleaning the liquid storage bin and the related liquid flow path;
The using method of the reaction mother liquor transfer comprises the following steps:
A1, placing a pipette into a reaction bottle after reaction, starting a peristaltic pump to rotate forward, pumping reacted mother liquor into a liquid storage bin, and clamping the pipette by a clamping piece to prevent dripping;
A2, placing the pipette into a receiving bottle, loosening the clamping piece from the pipette, starting a peristaltic pump to reverse, and discharging liquid in a liquid storage bin;
The using method of the liquid storage bin and the related liquid flow path rinsing comprises the following steps:
Step B1, pumping rinsing liquid into a liquid inlet pipeline, spraying the rinsing liquid A from a spray shower to a liquid storage bin for the first time, automatically rinsing the liquid storage bin and related liquid flow paths, and discharging the rinsing liquid into a receiving bottle;
step B2, the rinsing liquid A is sprayed to the liquid storage bin from the spray shower for the second time, the liquid storage bin and related liquid flow paths are automatically rinsed, and the rinsing liquid is discharged into the receiving bottle;
the using method of the liquid storage bin and related liquid flow path cleaning comprises the following steps:
step C1, placing a pipette into a waste liquid tank, pumping a cleaning liquid A into a liquid inlet pipeline, spraying the cleaning liquid A from a spray shower to a liquid storage bin, automatically rinsing the liquid storage bin and related liquid flow paths, and discharging the cleaning liquid into the waste liquid tank;
and C2, pumping a cleaning liquid B into a liquid inlet pipeline, spraying the cleaning liquid B from a spraying shower to a liquid storage bin, automatically rinsing the liquid storage bin 1 and related liquid flow paths, and discharging the cleaning liquid into a waste liquid tank.
The technical scheme provided by the multifunctional self-cleaning pipetting device has the following technical effects:
The peristaltic pump can be effectively prevented from being blocked, and the liquid including suspension, viscous liquid and clarified liquid can be transferred, stored and discharged;
The added liquid inlet pipeline and the spray shower can realize automatic cleaning, prolong the service lives of the liquid inlet pipeline, the liquid storage bin, the pipette and the adapter, and reduce the cost;
the single treatment capacity is large, 150 milliliters of liquid can be transferred, the structure is simple, and the use is convenient.
Drawings
FIG. 1 is a schematic diagram of a multifunctional self-cleaning pipetting device in accordance with the present invention;
Fig. 2 is a schematic diagram of a front view structure of a spray shower in the multifunctional self-cleaning pipetting device of the invention;
Fig. 3 is a schematic top view of a spray shower in the multifunctional self-cleaning pipetting device.
Wherein, the reference numerals are as follows:
liquid inlet pipeline 101, liquid storage bin 102, pipette 103, adapter 104, peristaltic pump 105, pump pipe 106, shower head 107, clamping piece 108 and opening 201.
Detailed Description
The invention is further described with reference to the following detailed description in order to make the technical means, the inventive features, the achieved objects and the effects of the invention easy to understand.
It should be understood that the structures, proportions, sizes, etc. shown in the drawings are for illustration purposes only and should not be construed as limiting the invention to the extent that it can be practiced, since modifications, changes in the proportions, or otherwise, used in the practice of the invention, are not intended to be critical to the essential characteristics of the invention, but are intended to fall within the spirit and scope of the invention.
Also, the terms such as "upper," "lower," "left," "right," "middle," and "a" and the like recited in the present specification are merely for descriptive purposes and are not intended to limit the scope of the invention, but are intended to provide relative positional changes or modifications without materially altering the technical context in which the invention may be practiced.
The invention provides a multifunctional self-cleaning pipetting device, which aims to effectively prevent a peristaltic pump from being blocked and realize the transfer, storage and discharge of liquid including suspension, viscous liquid and clarified liquid; the added liquid inlet pipeline and the spray shower can realize automatic cleaning, prolong the service lives of the liquid inlet pipeline, the liquid storage bin, the pipette and the adapter, and reduce the cost; the single treatment capacity is large, 150 milliliters of liquid can be transferred, the structure is simple, and the use is convenient.
As shown in fig. 1, a multifunctional self-cleaning pipetting device comprises a liquid inlet pipeline 101, a liquid storage bin 102, a pipette 103, an adapter 104, a peristaltic pump 105 and a pump pipe 106, wherein the bottom of the liquid storage bin 102 is connected with the pipette 103, the top of the liquid storage bin 102 is connected with the bottom of the adapter 104, a spray shower 107 is arranged in the liquid storage bin 102, the liquid inlet pipeline 101 penetrates through the adapter 104 and then is connected to the top of the spray shower 107, two ends of the pump pipe 106 are respectively connected to the top of the adapter 104 and the peristaltic pump 105, and a clamping piece 108 is inserted at one end of the pipette 103 close to the liquid storage bin 102.
The embodiment provides a multi-functional self-cleaning pipetting device, the top of stock solution storehouse 102 that adopts is equipped with the feed liquor pipe that is connected in adapter 104, and the first opening with the feed liquor pipe intercommunication has been seted up to adapter 104's bottom, and the second opening with pump line 106 intercommunication has been seted up at adapter 104's top.
The embodiment provides a multi-functional self-cleaning pipetting device, and the bottom of stock solution storehouse 102 that adopts is equipped with the drain pipe of being connected with pipette 103.
The liquid storage bin 102 includes a cylindrical tube body and an inverted cone tube body which are sequentially and fixedly connected from top to bottom.
The embodiment provides a multi-functional self-cleaning pipetting device, and the feed liquor pipe that adopts is the trapezoidal feed liquor pipe of falling.
The adapter 104 used in the multifunctional self-cleaning pipetting device provided in this embodiment is a glass frosted adapter 104.
The embodiment provides a multi-functional self-cleaning pipetting device, the chucking spare 108 that adopts is by inserting the T type chucking spare 108 of piece and knob piece fixed connection, is equipped with on the pipette 103 and supplies to insert the inserted through-hole of piece, and rotatory knob piece can be convenient for draw and insert the piece and make stock solution storehouse 102 and pipette 103 intercommunication and cut off, can prevent liquid leakage when transferring liquid because of gravity effect or misoperation.
As shown in fig. 2-3, in the multifunctional self-cleaning pipetting device provided in this embodiment, the adopted spray shower 107 is a cylindrical spray shower 107, the periphery of the cylindrical spray shower 107 is uniformly provided with a plurality of openings 201 communicated with the liquid inlet pipeline 101, when in use, cleaning liquid is conveyed to the spray shower 107 through the liquid inlet pipeline 101 to clean the inside of the liquid storage bin 102, and only a small amount of cleaning liquid is needed to clean the liquid storage bin 102, because in the traditional transferring mode, liquid enters from the pipette 103 at the bottom of the liquid storage bin 102 and is stored in the liquid storage bin 102, after the device moves to a receiving bottle, the liquid is discharged from the pipette 103, in this mode, 150 milliliters of reaction liquid is required to be transferred, at least 150 milliliters of cleaning liquid is needed, the cleaning liquid level is ensured to be the same as the liquid level to be transferred last time, and resources are very wasted.
In the multifunctional self-cleaning pipetting device provided in this embodiment, 50 openings 201 are provided, and the diameter of each opening 201 is 0.7 mm.
The multifunctional self-cleaning pipetting device provided by the embodiment has the following specific use conditions:
1. Transferring reaction mother liquor:
the purpose is to transfer the reaction liquid into a receiving bottle;
firstly, placing a pipette 103 into a reaction bottle after reaction, starting a peristaltic pump 105 to rotate forward, pumping reacted mother liquor into a liquid storage bin 102, clamping the pipette 103 by a clamping piece 108 to prevent dripping, placing the pipette 103 into a receiving bottle, loosening the pipette 103 by the clamping piece 108, starting the peristaltic pump 105 to rotate reversely, and discharging the liquid in the liquid storage bin 102;
2. Rinsing of the reservoir 102 and associated fluid flow path
The purpose is as follows: the liquid storage bin 102 and the residual reaction liquid in the liquid path are rinsed into a receiving bottle, so that the particle returning bin is ensured, and the liquid transfer rate is improved;
The liquid inlet pipeline 101 pumps rinsing liquid, the rinsing liquid A is sprayed from the spray shower 107 to the liquid storage bin 102 for the first time, the liquid storage bin 102 and related liquid flow paths are automatically rinsed, the rinsing liquid is discharged into a receiving bottle, the rinsing liquid A is sprayed from the spray shower 107 to the liquid storage bin 102 for the second time, the liquid storage bin 102 and related liquid flow paths are automatically rinsed, and the rinsing liquid is discharged into the receiving bottle;
3. cleaning of the reservoir 102 and associated fluid flow path
The purpose is as follows: cleaning and regenerating the liquid storage bin 102 and the liquid path;
The pipette 103 is placed in the waste liquid tank, the liquid inlet pipeline 101 pumps the cleaning liquid A, the cleaning liquid A is sprayed from the spraying shower 107 to the liquid storage bin 102, the liquid storage bin 102 and related liquid flow paths are automatically rinsed, the cleaning liquid is discharged into the waste liquid tank, the liquid inlet pipeline 101 pumps the cleaning liquid B, the cleaning liquid B is sprayed from the spraying shower 107 to the liquid storage bin 102, the liquid storage bin 102 and related liquid flow paths are automatically rinsed, and the cleaning liquid is discharged into the waste liquid tank.
In conclusion, the multifunctional self-cleaning pipetting device can effectively prevent a peristaltic pump from being blocked, and realize transfer, storage and drainage of liquids including suspension, viscous liquid and clarified liquid; the added liquid inlet pipeline and the spray shower can realize automatic cleaning, prolong the service lives of the liquid inlet pipeline, the liquid storage bin, the pipette and the adapter, and reduce the cost; the single treatment capacity is large, 150 milliliters of liquid can be transferred, the structure is simple, and the use is convenient.
The foregoing describes specific embodiments of the invention. It is to be understood that the invention is not limited to the specific embodiments described above, wherein devices and structures not described in detail are to be understood as being implemented in a manner common in the art; numerous variations, changes, or substitutions of light can be made by one skilled in the art without departing from the spirit of the invention and the scope of the claims.
Claims (8)
1. The multifunctional self-cleaning pipetting device is characterized by comprising a liquid inlet pipeline, a liquid storage bin, a pipette, an adapter, a peristaltic pump and a pump pipe, wherein the bottom of the liquid storage bin is connected with the pipette, the top of the liquid storage bin is connected with the bottom of the adapter, a spray shower is arranged in the liquid storage bin, the liquid inlet pipeline penetrates through the adapter and then is connected to the top of the spray shower, two ends of the pump pipe are respectively connected to the top of the adapter and the peristaltic pump, and a clamping piece is inserted into one end, close to the liquid storage bin, of the pipette;
The top of the liquid storage bin is provided with a liquid inlet pipe connected with the adapter, the bottom of the adapter is provided with a first port communicated with the liquid inlet pipe, and the top of the adapter is provided with a second port communicated with the pump pipe;
The spray shower is arranged below the liquid inlet pipe, and the liquid inlet pipe is an inverted trapezoid liquid inlet pipe.
2. The multifunctional self-cleaning pipetting device of claim 1 wherein the bottom of the reservoir is provided with a drain tube connected to the pipette.
3. The multifunctional self-cleaning pipetting device of claim 2 wherein the reservoir comprises a cylindrical tube and a back taper tube fixedly connected in sequence from top to bottom.
4. The multi-functional self-cleaning pipetting device of claim 1 wherein the adapter is a glass frosted adapter.
5. The multifunctional self-cleaning pipetting device of claim 1 wherein the gripping member is a T-shaped gripping member fixedly connected by an insertion block to a knob block, and the pipette is provided with a through hole for insertion of the insertion block.
6. The multifunctional self-cleaning pipetting device of claim 1 wherein the spray shower is a cylindrical spray shower, and a plurality of openings communicated with the liquid inlet pipeline are uniformly formed in the periphery of the cylindrical spray shower.
7. The multi-purpose self-cleaning pipetting device of claim 6 wherein there are 50 openings each having a diameter of 0.7 mm.
8. A method for using the multifunctional self-cleaning pipetting device, which is characterized by using the multifunctional self-cleaning pipetting device as claimed in claim 1, wherein the method comprises a reaction mother liquor transferring method, a liquid storage bin and a related liquid flow path rinsing method and a liquid storage bin and a related liquid flow path cleaning method;
The using method of the reaction mother liquor transfer comprises the following steps:
A1, placing a pipette into a reaction bottle after reaction, starting a peristaltic pump to rotate forward, pumping reacted mother liquor into a liquid storage bin, and clamping the pipette by a clamping piece to prevent dripping;
A2, placing the pipette into a receiving bottle, loosening the clamping piece from the pipette, starting a peristaltic pump to reverse, and discharging liquid in a liquid storage bin;
The using method of the liquid storage bin and the related liquid flow path rinsing comprises the following steps:
Step B1, pumping rinsing liquid into a liquid inlet pipeline, spraying the rinsing liquid A from a spray shower to a liquid storage bin for the first time, automatically rinsing the liquid storage bin and related liquid flow paths, and discharging the rinsing liquid into a receiving bottle;
step B2, the rinsing liquid A is sprayed to the liquid storage bin from the spray shower for the second time, the liquid storage bin and related liquid flow paths are automatically rinsed, and the rinsing liquid is discharged into the receiving bottle;
the using method of the liquid storage bin and related liquid flow path cleaning comprises the following steps:
step C1, placing a pipette into a waste liquid tank, pumping a cleaning liquid A into a liquid inlet pipeline, spraying the cleaning liquid A from a spray shower to a liquid storage bin, automatically rinsing the liquid storage bin and related liquid flow paths, and discharging the cleaning liquid into the waste liquid tank;
and C2, pumping a cleaning liquid B into a liquid inlet pipeline, spraying the cleaning liquid B from a spraying shower to a liquid storage bin, automatically rinsing the liquid storage bin 1 and related liquid flow paths, and discharging the cleaning liquid into a waste liquid tank.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910728165.3A CN110404597B (en) | 2019-08-08 | 2019-08-08 | Multifunctional self-cleaning pipetting device and application method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910728165.3A CN110404597B (en) | 2019-08-08 | 2019-08-08 | Multifunctional self-cleaning pipetting device and application method thereof |
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| CN110404597A CN110404597A (en) | 2019-11-05 |
| CN110404597B true CN110404597B (en) | 2024-08-16 |
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| CN111589480B (en) * | 2020-05-29 | 2024-08-06 | 成都瀚辰光翼科技有限责任公司 | Pipetting device, microsystem pipetting system comprising pipetting device and method thereof |
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