CN110361538A - A fully automatic western antibody incubation and washing machine that can be remotely controlled by mobile APP - Google Patents

Abstract

The invention discloses a fully automatic western antibody incubation and washing machine that can be remotely controlled by a mobile phone APP, including an antibody storage module, an antibody incubation and washing module, a washing liquid supply and waste liquid recovery module, and a mobile phone APP remote operation and control module. The invention enables experimental operators to conduct experimental control through mobile phones at home or on the way to work, automates the frequently operated procedures, and saves manpower for other tasks.

Description

A fully automatic western antibody incubation and washing machine that can be remotely controlled by mobile APP

technical field

The invention belongs to the field of experimental equipment manufacturing, and relates to a fully automatic washing system, in particular to a fully automatic western antibody incubation and washing machine which can be remotely controlled by a mobile APP.

Background technique

In the scientific research and production fields of life science, biomedicine and agriculture and forestry science, western blotting (immunoblotting test) is Western Blot, which is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. The basic principle is to stain the cells or biological tissue samples treated by gel electrophoresis through specific antibodies. Information about the expression of specific proteins in the analyzed cells or tissues can be obtained by analyzing the location and depth of staining. In the well-known western-blot experiment for protein detection, the protein is transferred to polyvinylidene fluoride (PVDF) membrane through high current, and then the protein is bound to the specific antibody, through antibody binding-free antibody washing-secondary antibody binding - Detection by chemiluminescence and other steps. In this process, specific antibody incubation usually needs to be carried out at 4-8°C for 16-20 hours. The washing of the transfer membrane is usually repeated at room temperature for a short time. The process requires manual repeated replacement of antibodies or washing solutions, which consumes a lot of time and manpower. At present, the western membrane washing machines appearing on the market are designed to meet the convenience needs of the local experimental operation process in the scientific research and experimental fields such as life sciences. However, in actual use, the problem of labor waste in western-blot experiments has not been solved due to the limitations of the experimental schedule and other shortcomings, and the antibody incubation and washing process is not continuous and cannot be programmed, and the operator must be within a certain period of time. The operation is performed in person at the site, and antibody incubation needs to be carried out by placing shakers and other equipment in a refrigerator at 2-8°C, which poses safety hazards and greatly limits the coordinated development of time, space, and people in scientific research.

Contents of the invention

The purpose of the present invention is to provide a fully automatic western antibody incubation and washing machine that can be remotely controlled by mobile phone APP to overcome the problems existing in the prior art. Automate frequently-operated procedures, saving manpower for other tasks.

To achieve the above object, the present invention adopts the following technical solutions:

A fully automatic western antibody incubation and washing machine that can be remotely controlled by a mobile phone APP, including an antibody storage module, an antibody incubation and washing module, a washing liquid supply and waste liquid recovery module, and a mobile phone APP remote operation and control module;

The antibody storage module includes a tube rack with good thermal conductivity. Several antibody tube slots are arranged on the tube rack. Antibody tubes are arranged in the antibody tube slots. Pipeline; the antibody incubation and washing module includes an incubation tank with good thermal conductivity, the incubation tank is provided with a liquid input interface and a liquid output interface, and the tube rack and the lower side of the incubation tank are respectively provided with a first refrigeration system and a second refrigeration system. system, the lower part of the second refrigeration system is also provided with an electric motor capable of tilting and swinging the incubation tank; the washing liquid supply and waste liquid recovery module includes a washing liquid bottle and a waste liquid recovery bottle, the outlet end of the antibody tube and the washing liquid bottle The outlet port of the outlet port is connected to the liquid input port through the first liquid pump and the fourth liquid pump respectively, and the liquid output port is connected to the inlet port of the waste liquid recovery bottle and the inlet port of the antibody tube through the third liquid pump and the second liquid pump respectively ;

The mobile phone APP remote operation and control module includes an operation control panel, a mobile phone APP remote control transmitter and a power switch, the first refrigeration system, the second refrigeration system and the electric motor are all connected to the operation control panel, and the mobile phone APP remote control transmitter Connect to the mobile terminal through the WiFi port.

Further, the tube rack is provided with 6 antibody tube slots, which are the first antibody tube slot for placing the first antibody tube, the second antibody tube slot for placing the second antibody tube, and the third antibody tube slot for placing the third antibody tube. the third antibody tube slot for the tube, the fourth antibody tube slot for the fourth antibody tube, the fifth antibody tube slot for the fifth antibody tube and the sixth antibody tube slot for the sixth antibody tube, The non-specific antigen blocking solution is placed in the first antibody tube, the first antibody is placed in the second antibody tube, the second antibody is placed in the third antibody tube, the fourth antibody tube, the fifth antibody tube and the sixth antibody tube are pre-prepared. Reserve.

Further, the first refrigeration system and the second refrigeration system both include a refrigeration compressor and a condensation pipe connected to the refrigeration compressor, the first refrigeration system also includes a first temperature sensor arranged at the bottom of the pipe frame, and the second refrigeration system It also includes a second temperature sensor arranged at the bottom of the incubation tank, and both the first temperature sensor and the second temperature sensor are connected to the operation control panel.

Further, the temperature adjustment range of the first refrigeration system is 2-8°C, and the temperature adjustment range of the second refrigeration system is 2°C-room temperature.

Further, the washing liquid supply and waste liquid recovery module includes a first washing liquid bottle, a second washing liquid bottle and a waste liquid recovery bottle, the first washing liquid bottle is used for holding the washing liquid TBS-T, and the second washing liquid bottle is used for It is used to hold the cleaning solution TBS, and the waste liquid recovery bottle is used to recycle the used cleaning solution.

Further, the volumes of the first washing liquid bottle and the second washing liquid bottle are both 500 mL, and the volume of the waste liquid recovery bottle is 2 L.

Further, liquid level sensors are provided in the first washing liquid bottle, the second washing liquid bottle and the waste liquid recovery bottle, and the liquid level sensors are connected to the operation control panel.

Further, the operation control panel includes the following control and display interfaces: antibody incubation temperature: range from 2°C to room temperature; antibody incubation time: 0-24 hours, accurate to minutes; antibody incubation swing speed: 0-200 rpm; Membrane washing temperature: 2℃-room temperature range; Membrane washing time: 0-24 hours, accurate to minutes; Membrane washing swing speed: 0-200 rpm; Liquid level display of washing liquid bottle and waste liquid recovery bottle.

Further, the pipeline between the outlet end of the antibody tube and the first liquid pump, the pipeline between the outlet end of the washing liquid bottle and the fourth liquid pump, the pipeline between the first liquid pump and the fourth liquid pump and the liquid input interface, the liquid The pipes between the output interface and the third liquid pump and the second liquid pump, the pipes between the third liquid pump and the inlet of the waste liquid recovery bottle, and the pipes between the second liquid pump and the inlet of the antibody tube are all made of acid and alkali resistant Corroded silicone tubing.

Further, on the pipeline between the outlet end of the antibody tube and the first liquid pump, on the pipeline between the outlet end of the washing liquid bottle and the fourth liquid pump, and on the pipeline between the third liquid pump and the inlet end of the waste liquid recovery bottle And the pipeline between the second liquid pump and the inlet end of the antibody tube is provided with a solenoid valve for controlling the opening and closing of the pipeline.

Compared with the prior art, the present invention has the following beneficial technical effects:

The invention mechanizes tedious labor such as incubation and membrane washing in the western-blot experiment process, such as manual frequent replacement of liquids, saves manpower for other experiments or data statistics, and also avoids human interference factors in the manual operation process, ensuring Experimental stability and repeatability can also ensure the effectiveness of BSA and antibodies and save antibodies. In addition, the invention is simple in batch production, easy to operate, low in cost, low in energy consumption, and widely used.

Furthermore, the present invention integrates the processes of non-specific antigen blocking, incubation of the first antibody at 2-8°C, washing with TBS-T washing solution, and incubation of the second antibody at room temperature in the western-blot experiment process through automatic control, and uses solenoid valve control Pipeline switches and liquid pumps realize the automatic input and output of antibodies, washing liquid and other liquids according to the programming procedures, and control the temperature of antibody storage and incubation through the refrigeration system to ensure the stability of antibody titers during the experiment and the accuracy of the experiment. The operation function of the intelligent control panel can be transmitted to the mobile phone APP through wireless WiFi for remote operation, reducing the on-site operation of experimenters within a certain period of time.

Description of drawings

Fig. 1 is a schematic plan view of the overall top view of the present invention;

Figure 2 is a schematic diagram of the front structure of the antibody storage module of the present invention;

3 is a schematic diagram of the front structure of the antibody incubation and washing module of the present invention;

Fig. 4 is a schematic diagram of the front structure of the washing liquid supply and waste liquid recovery module of the present invention;

Fig. 5 is a schematic diagram of the side structure of the remote operation and control module of the present invention;

Fig. 6 is a schematic diagram of the liquid pipeline connection of the present invention.

Among them, 1 is the antibody storage module; 2 is the antibody incubation and washing module; 3 is the washing solution supply and waste liquid recovery module; 4 is the mobile phone APP remote operation and control module; 5 is the antibody tube rack; 6 is the first antibody tube tank ; 7 is the second antibody tube tank; 8 is the third antibody tube tank; 9 is the fourth antibody tube tank; 10 is the fifth antibody tube tank; 11 is the sixth antibody tube tank; 12 is the incubation tank; 13 is the liquid input Interface; 14 is the liquid output interface; 15 is the first washing liquid bottle; 16 is the second washing liquid bottle; 17 is the waste liquid recovery bottle; 18 is the mobile phone APP remote control transmitter; 19 is the operation control panel; 20 is the power switch ; 21 is the first refrigeration system; 22 is the second refrigeration system; 23 is the electric motor; 24 is the connecting rod; 25 is the liquid level sensor; 26 is the refrigeration compressor; M1 is the first liquid pump; M2 is the second liquid pump ; M3 is the third liquid pump; M4 is the fourth liquid pump; K1-K9 are solenoid valves.

Detailed ways

Below in conjunction with accompanying drawing, the present invention is described in further detail:

See Figures 1 to 5, a fully automatic western antibody incubation and washing machine that can be remotely controlled by a mobile phone APP, including an antibody storage module 1, an antibody incubation and washing module 2, a washing liquid supply and waste liquid recovery module 3, and a mobile phone APP remote control module. Operation and control module4.

The antibody storage module 1 is composed of 6 antibody tubes and an alloy tube frame 5 with good thermal conductivity, and a first refrigeration system 21 is provided under the tube frame 5 . The antibody incubation and washing module 2 is composed of an incubation tank 12 made of an alloy with good thermal conductivity for incubating antibodies or washing liquids, etc. The incubation tank 12 is provided with a liquid input interface 13 and a liquid output interface 14. The following design An electric motor 23 drives the incubation tank 12 to tilt up and down at a certain angle, and a second cooling system 22 is provided under the antibody incubation and washing module 2 to maintain the incubation temperature. The washing liquid supply and waste liquid recovery module 3 is composed of a first washing liquid bottle 15 with a volume of 500ml, a second washing liquid bottle 16 and a 2L waste liquid recovery bottle 17. The system is equipped with a micro liquid pump to pump liquid out and recycling.

The mobile phone APP remote operation and control module 4 includes a manually operable control panel 19 and a mobile phone APP remote control transmitter 18, which is equipped with a wireless wifi port, which can independently set and control the antibody incubation or membrane washing temperature and temperature of the incubation tank 12. The time, the electric motor drives the antibody incubation and the swing speed of the washing module 2, and the experimental steps can be programmed.

The present invention integrates the antibody incubation and washing of the transfer membrane during the western-blot experiment. The antibody storage module 1 and the antibody incubation and washing module 2 are respectively equipped with a first refrigeration system 21 and a second refrigeration system 22, and independently control the temperature, all The operation can be remotely programmed and operated through the mobile phone APP, and the remote control transmitter 18 of the mobile phone APP is equipped with wireless WiFi, which can be connected to the local area network, and the experimental operation can be performed remotely through the mobile phone APP software interface; the antibody storage module 1 is in the Place a tube rack made of an alloy material with strong thermal conductivity in the environment provided by the first refrigeration system 21 at a temperature of 2-8°C, which can hold 50ml antibody tubes, and the antibody tube cover is provided with an antibody pump-out and pump-in interface for connecting liquids The pump, the antibody incubation and washing module 2 is driven by the electric motor 23 to tilt up and down at a certain angle, and the second refrigeration system 22 is set on the platform of the antibody incubation and washing module 2 to provide a temperature of 2°C-room temperature environment, the second refrigeration An incubation tank made of an alloy material with strong thermal conductivity is placed on the system 22 and is equipped with a highly airtight tank cover. The incubation tank is provided with a liquid input interface 13 and a liquid output interface 14, which are respectively used to connect the liquid pump and the antibody. Storage module 1, washing liquid bottle and waste liquid recovery module 3; said washing liquid supply and waste liquid recovery module 3 are composed of two first washing liquid bottles 15 and second washing liquid bottles 16 with a volume of 500ml and a 2L washing liquid bottle The waste liquid recovery bottle 17 is composed of a bottle cap with a liquid pumping or recovery interface for connecting the liquid pump, and is provided with a liquid level sensor 25; the APP remote operation and control module 4, and the operation control interface of the instrument itself includes: Antibody incubation temperature (adjustable from 2°C to room temperature), antibody incubation time (adjustable from 0 to 24 hours, accurate to minutes), antibody incubation swing speed (0-200 rpm); membrane washing temperature (2°C to room temperature Adjustable range), membrane washing time (adjustable from 0-24 hours, accurate to minutes), membrane washing swing speed (0-200 rpm); liquid level display of washing liquid bottle and waste liquid recovery bottle; programmable 0- 99 sets of experimental programs, the system program has automatic memory function, can automatically start the program and continue the unfinished program after the power failure is restored, all connecting pipelines are made of acid-base corroded silicone tubes, and the pipelines are opened or closed by electromagnetic Relay control, all liquid containers can be easily disassembled and washed.

The present invention is described in detail below in conjunction with embodiment:

A fully automatic western antibody incubation and washing machine that can be remotely controlled by a mobile phone APP. It consists of four parts, including: antibody storage module 1, antibody incubation and washing module 2, antibody incubation and washing module 3, washing liquid supply and waste liquid Recycling module 4. The antibody storage module includes a tube rack 5 with good thermal conductivity and 6 antibody tubes. The antibody tubes can be conveniently placed in the tube rack 5. Two hose interfaces are fixed on the antibody tube cover, which are respectively connected to the antibody incubation and washing modules. The liquid input interface 13 of 2, the liquid output interface 14 are connected with the liquid pump, and the first refrigeration system 21 is provided below the pipe frame 5. The antibody incubation and washing module 2 includes an antibody incubation tank 12, the two ends of the incubation tank 12 are respectively provided with a liquid input interface 13, a liquid output interface 14, and the liquid input interface 13 and the liquid output interface 14 are respectively connected to the antibody tube of the antibody storage module 1. , washing liquid supply and waste liquid recovery module 3, the washing liquid bottle, the waste liquid recovery bottle, and the liquid pump are connected, and a second refrigeration system 22 is provided under the antibody incubation tank 12, and there is a piece of supporting metal below the second refrigeration system 22 Plate, fixed on the electric motor 23. The refrigeration system consists of a refrigeration compressor, a condensing pipe, and a temperature sensor. The mobile phone APP remote operation and control module 4 is a central processing unit, which controls the antibody storage module 1, antibody incubation and washing module 2, washing liquid supply and waste liquid recovery module 3 according to the automatic program, and sends the program to the mobile phone through WiFi APP remote operation.

Working principle of the present invention is:

When in use, add 5% bovine serum albumin (BSA) to the first antibody tube in advance, add the primary antibody to the second antibody tube, and add the secondary antibody to the third antibody tube (the other three antibody tubes are for use). A washing liquid bottle 15 is filled with washing liquid (TBS-T), and a second washing liquid bottle 16 is filled with washing liquid (TBS). After the Western transfer in the previous step of the experiment is completed, the transfer membrane is placed in the antibody incubation tank 12 . Set the experimental program on the interface of the mobile phone APP remote operation and control module 4 or the mobile phone APP interface, for example, the first step: 5% BSA, 60 minutes, 30 °C; the second step: mouse anti-human primary antibody, 18 hours, 4°C; Step 3: First wash with washing solution (TBS-T), 5 minutes, 30°C; Step 4: Second wash with washing solution (TBS-T), 5 minutes, 30°C; Step 5 : Washing solution (TBS-T) for the third wash, 5 minutes, 30°C; Step 6: HRP-goat anti-mouse secondary antibody, 60 minutes, 30°C; Step 7: Washing solution (TBS-T) First wash, 10 minutes, 30°C; eighth step: second wash with washing solution (TBS-T), 10 minutes, 30°C; ninth step: third wash with washing solution (TBS-T), 10 minutes , 30°C. Confirm the program setting end speed, and then start the program. After the entire program is completed, the pipeline cleaning function will be automatically activated, and the pipeline will be cleaned with cleaning solution (TBS). In order to form a cross-contamination-free pipeline, prepare for the next experiment. In this part of the experiment, 5% BSA, antibody, and membrane washing solution will be automatically recovered into the original antibody tube, and the washing solution will be automatically recovered into the waste liquid recovery bottle.

Referring to Fig. 6, the present invention automatically integrates the following experimental procedures after completing the transfer step of the previous stage in the western-blot experiment. According to the needs of the experimental design, the first step: the first liquid pump M1 is started, the solenoid valve K1 is opened, and the other solenoid valves are all closed, and the non-specific antigen blocking solution (5% BSA) in the primary antibody tube is pumped into the incubation tank 12 After reaching the set liquid volume, the first liquid pump M1 stops, the solenoid valve K1 is closed, the electric motor 23 is started, and the incubator is driven by the connecting rod 24 to tilt and swing, and the incubation is carried out at room temperature for 1 hour; then the second liquid pump M2 is automatically started, At the same time, only the solenoid valve K2 is opened, and the 5% BSA in the incubation tank 12 is recovered into the primary antibody tube, and the antigen blocking is completed at this time;

Step 2: start the first liquid pump M1, open the solenoid valve K2, and close the rest of the solenoid valves. Pump the first antibody on the second antibody tube into the incubation tank 12. After reaching the set liquid volume, the first liquid pump M1 stops, the solenoid valve K2 is closed, the electric motor 23 is started, and the incubator is tilted and swayed by the connecting rod 24, and incubated at 4°C for 18 hours; then the second liquid pump M2 is automatically started, and only the solenoid valve K5 is opened at the same time, and the incubator The primary antibody in 12 was recovered into the secondary antibody tube 7. Then the fourth liquid pump M4 is started, the electromagnetic valve K7 is opened, and the rest of the electromagnetic valves are all closed, and the washing liquid in the first washing liquid bottle 15 is pumped into the incubation tank 12, and the fourth liquid pump M4 stops after reaching the set liquid amount. , the electromagnetic valve K7 is closed, the electric motor 23 is started, and the incubating tank is tilted and swayed by the connecting rod 24, and incubated at room temperature for 5 minutes; then the third liquid pump M3 is automatically started, and only the electromagnetic valve K9 is opened at the same time, and the incubating tank 12 is washed. The liquid is recovered in the waste liquid recovery bottle 17, and this process is repeated for washing 3 times. At this point, the primary antibody incubation step is complete;

Step 3: start the first liquid pump M1, open the solenoid valve K3, and close the other solenoid valves. Pump the second antibody on the third antibody tube into the incubation tank 12. After reaching the set liquid volume, the first liquid pump M1 stops, the solenoid valve K3 is closed, the electric motor 23 is started, and the incubator is tilted and swayed by the connecting rod 24, and incubated at room temperature for 1 hour; then the second liquid pump M2 is automatically started, and at the same time, only the solenoid valve K6 is opened, and the incubator 12 The secondary antibody was recovered into the antibody tube. Then the fourth liquid pump M4 is started, the electromagnetic valve K7 is opened, and the rest of the electromagnetic valves are all closed, and the washing liquid in the first washing liquid bottle 15 is pumped into the incubation tank 12, and the fourth liquid pump M4 stops after reaching the set liquid amount. , the electromagnetic valve K7 is closed, the electric motor 23 is started, and the incubating tank is tilted and swayed by the connecting rod 24, and incubated at room temperature for 10 minutes; then the third liquid pump M3 is automatically started, and only the electromagnetic valve K9 is opened at the same time, and the incubating tank 12 is washed. The liquid is recovered in the waste liquid recovery bottle 17, and this process is repeated for washing 3 times. At this time, the secondary antibody incubation step is completed, and the membrane is stored in the incubation tank or taken out for the next experiment; at this time, the entire western antibody incubation and washing process is automatically completed.

Start the pipeline and incubator cleaning program, the third liquid pump M3 and the fourth liquid pump M4 are started at the same time, the solenoid valve K8 and the solenoid valve K9 are opened, and the pipeline is cleaned for use by the next group.

Claims (10)

1. one kind can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application, which is characterized in that including antibody Storage module (1), antibody incubation and wash module (2), cleaning solution supply and devil liquor recovery module (3) and cell phone application are remotely grasped Work and control module (4)；

The antibody storage module (1) includes the good pipe support of heating conduction (5), and several antibody tube seats are provided on pipe support (5), Antibody pipe is provided in antibody tube seat, the pipe of antibody pipe, which is covered, is respectively used to the pipeline that antibody is output and input equipped with two；Institute It states antibody incubation and wash module (2) includes the good incubation slot of heating conduction (12), it is defeated to be provided with liquid on incubation slot (12) The first refrigeration system is respectively arranged on the downside of incoming interface (13) and liquid output interface (14), pipe support (5) and incubation slot (12) (21) and the second refrigeration system (22), the second refrigeration system (22) lower part, which is additionally provided with, can make incubation slot (12) wallowing motion Electric motor (23)；The cleaning solution supply and devil liquor recovery module (3) include Washing liquid bottle and devil liquor recovery bottle (17), are resisted The outlet end of body pipe and the outlet end of Washing liquid bottle pass through the first liquid pump (M1) respectively and the 4th liquid pump (M4) is connected to liquid Body input interface (13), liquid output interface (14) by passing through third liquid pump (M3) and second liquid pump (M2) connection respectively To the arrival end of devil liquor recovery bottle (17) and the arrival end of antibody pipe；

The cell phone application remotely operates and control module (4) is including operating control panel (19), cell phone application remotely controls hair Emitter (18) and power switch (20), the first refrigeration system (21), the second refrigeration system (22) and electric motor (23) are all connected with To operation control panel (19), cell phone application remote control transmitter (18) is connect by the port WiFi with mobile terminal.

2. one kind according to claim 1 can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application, It is characterized in that, 6 antibody tube seats are provided on pipe support (5), respectively for placing the first antibody tube seat of first antibody pipe (6), for place the secondary antibody tube seat (7) of secondary antibody pipe, the third antibody tube seat (8) for placing third antibody pipe, For placing the 4th antibody tube seat (9), the 5th antibody tube seat (10) for placing the 5th antibody pipe and use of the 4th antibody pipe Heterogenetic antigen confining liquid, second are placed in the 6th antibody tube seat (11) for placing the 6th antibody pipe, first antibody pipe It is placed with first antibody in antibody pipe, is placed with secondary antibody, the 4th antibody pipe, the 5th antibody pipe and the 6th in third antibody pipe Antibody pipe is reserved spare.

3. one kind according to claim 1 can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application, It is characterized in that, first refrigeration system (21) and the second refrigeration system (22) include refrigeration compressor (26) and with system The condenser pipe of cold compressor (26) connection, the first refrigeration system (21) further include that first temperature of the setting in pipe support (5) bottom passes Sensor, the second refrigeration system (22) further includes second temperature sensor of the setting in incubation slot (12) bottom, and the first temperature passes Sensor and second temperature sensor are connected to operation control panel (14).

4. one kind according to claim 1 can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application, It is characterized in that, the temperature regulating range of the first refrigeration system (21) is 2-8 DEG C, the temperature of the second refrigeration system (22) adjusts model It encloses for 2 DEG C-room temperature.

5. one kind according to claim 1 can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application, It is characterized in that, cleaning solution supply and devil liquor recovery module (3) include the first Washing liquid bottle (15), the second Washing liquid bottle (16) and One devil liquor recovery bottle (17), the first Washing liquid bottle (15) is for containing cleaning solution TBS-T, and the second Washing liquid bottle (16) is for containing Cleaning solution TBS is filled, devil liquor recovery bottle (17) is for recycling used cleaning solution.

6. one kind according to claim 5 can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application, It is characterized in that, the volume of the first Washing liquid bottle (15) and the second Washing liquid bottle (16) is 500mL, devil liquor recovery bottle (17) Volume is 2L.

7. one kind according to claim 5 can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application, It is characterized in that, being provided with liquid level biography in the first Washing liquid bottle (15), the second Washing liquid bottle (16) and devil liquor recovery bottle (17) Sensor (25), and liquid level sensor (25) is connected to operation control panel (19).

8. one kind according to claim 7 can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application, It is characterized in that, the operation control panel (19) includes with down regulation and display interface: antibody incubation temperature: 2 DEG C-room temperature model It encloses；The antibody incubation time: 0-24 hours, it is accurate to minute；Antibody incubation swing speed: 0-200 revs/min；Wash film temperature: 2 DEG C-room temperature range；It washes the film time: 0-24 hours, being accurate to minute；Wash film swing speed: 0-200 revs/min；Washing liquid bottle and Devil liquor recovery bottle liquid level is shown.

9. one kind according to claim 1 can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application, It is characterized in that, pipeline, the outlet end of Washing liquid bottle and the 4th liquid between the outlet end of antibody pipe and the first liquid pump (M1) Pump pipeline between (M4), pipeline, liquid between the first liquid pump (M1) and the 4th liquid pump (M4) and liquid input interface (13) Pipeline, third liquid pump (M3) and devil liquor recovery between output interface (14) and third liquid pump (M3) and second liquid pump (M2) Pipeline is all made of acid and alkali-resistance between pipeline and second liquid pump (M2) and the arrival end of antibody pipe between the arrival end of bottle (17) The silicone tube of corrosion.

10. one kind according to claim 9 can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application, It is characterized in that, between the outlet end of antibody pipe and the first liquid pump (M1) on pipeline, the outlet end of Washing liquid bottle and the 4th liquid Body pumps between (M4) on pipeline, between third liquid pump (M3) and the arrival end of devil liquor recovery bottle (17) on pipeline and the second liquid The solenoid valve for controlling pipeline opening and closing is provided between body pump (M2) and the arrival end of antibody pipe on pipeline.