CN110333345A - A magnetic focusing rapid detection system with elution function - Google Patents

Abstract

The invention discloses a magnetic focusing rapid detection system, which comprises a base, a detection box and an elution box. There is a groove on the base with the same width as the detection box, and a first magnet and a second magnet are arranged in the groove; there are test papers in the detection box; There are vacuole, nitrocellulose film and absorbent paper in the elution box; the first magnet is in alignment with the development of the control line of the test paper, the second magnet is in alignment with the development of the detection line of the test paper, and the test paper is located on the nitrocellulose film. Between the absorbent paper and the edge of the test paper, the edge of the nitrocellulose film and the edge of the absorbent paper are attached to each other. The invention prolongs the reaction time, greatly improves the detection sensitivity, and sets a washing box to simplify the washing operation.

Description

A magnetic focusing rapid detection system with elution function

technical field

The invention relates to a rapid detection system.

Background technique

The content of the background technology is only to help understand the technical content, not the prior art.

Flow immunoassay (Lateral flow immunoassay, LFIA) is a practical, simple and effective rapid pathogen screening technology. LFIA sensors have been used to detect objects visible to the naked eye with visual signals. Typically, an untrained person can complete an LFIA within 15 minutes. However, a key limitation of LFIA is low sensitivity. For the whole pathogen detection, the limit of detection (LOD) of conventional LFIA is in the range of 104-106 CFU/mL. In order to improve the sensitivity of lateral flow immunoassays, scientists have proposed detection methods based on fluorescent probes, which require additional instruments to read the signal and also require additional steps, such as DNA/ RNA enrichment and amplification from pre-culture. Analysis of bacterial indicators such as intercellular enzymes or environmental chemicals that live bacteria need to consume is also used in low-frequency based detection to improve sensitivity, but requires a long detection time. Stimulation by horseradish peroxidase (HRP) can enhance the detection of DNA and protein by LF, so in our previous work, we introduced an enzyme amplification strategy to achieve an LOD of about 100 CFU/mL by HRP catalyzing the reaction value. However, food safety standards are stringent and require stringent protocols and monitoring standards. Therefore, the requirements for sensor specifications are also stringent, and they need to be highly sensitive, specific, fast and simple, and have the potential for on-site detection.

One of the main reasons for the poor sensitivity of the LFIA platform is the limited interaction of the probe-labeled pathogen with the capture antibody immobilized in the detection zone due to the low surface reaction rate. Since the flow of LF strips is based on capillary action, the labeled pathogens will be controlled by the material properties, so it is difficult to control the interaction time between the labeled target and the capture antibody. The limited interaction time of the target with the capture probe results in a low proportion of target captured. To overcome this key weakness, the scientists turned to an electrophoretic technique called isotope electrophoresis (ITP), which can preconcentrate and control the movement of targets by adjusting the electrical current. The LOD of LFIA systems can be improved by using ITP to slow down the movement of targets to increase the interaction time between elements. However, additional instrumentation is required to control the current in the strip, which cannot meet the simplicity requirement for LFIA. Among other things, Arben et al. (2014) printed wax column patterns on LF tapes, using pseudo-turbulence effects to reduce the flow rate and prolong the reaction time. They also use LF strips of different shapes to control the flow rate. However, the improved LOD based on these simple strategies did not significantly improve the detection of low-concentration targets.

A major reason for the low detection sensitivity of the current LFIA platform is the limited interaction between the probe-labeled pathogen and the capture antibody immobilized at the detection zone due to the low surface reaction rate. Fluid flow through the LF strip is based on capillary action, and the movement of probe-labeled pathogens is limited by material properties, so it is difficult to control the interaction time between the labeled target and the capture antibody. That is to say, the limited interaction time between the target and the capture probe results in a low capture rate of the target. To solve this technical problem, researchers have used isotachophoresis to slow down the movement of the target and increase the interaction time by adjusting the current enrichment and controlling the movement of the target. However, this method requires additional equipment to control the band On the contrary, the detection operation is more complicated. In order to prolong the interaction time, there have been other attempts such as spraying wax columns on the LF strips and changing the shape of the strips, but the effect is not obvious.

The applicant uses magnetic modification to increase the interaction time of the target object in the detection area on the strip to achieve a relatively low detection limit, and at the same time installs a washing box to simplify the cleaning steps. However, there is currently no kit for rapid detection with a magnetically modified target and a washing box.

Contents of the invention

The purpose of the present invention is to provide a rapid detection system that uses magnetic modification to increase the interaction time of the target on the detection area of the strip, and installs a washing box to simplify the cleaning steps and achieve a relatively low detection limit.

The present invention adopts following technical scheme to realize:

A magnetic focusing rapid detection system, characterized in that it includes a base, a detection box and an elution box, the base has a groove matching the detection box, and a first magnet and a second magnet are arranged in the groove; the detection box includes a box body and a detection test paper , the detection test paper is fixed on the box body, and the developing part of the test paper is exposed outside the box body; the elution box is provided with bubbles storing washing liquid, deflectors and absorbent paper, and the distance between the deflectors and the absorbent paper is related to the detection The test paper is matched; the first magnet is in alignment with the developed position of the control line of the test paper, and the second magnet is aligned with the developed position of the test line of the test paper.

When in use, first put the test box into the groove of the base, the bottom surface of the box body of the test box is attached to the base, the developing area of the test paper is exposed outside the box, and the control line and the test line of the test paper are aligned with the first magnet and the test line respectively. The second magnet: add samples to the detection test paper, after developing, cover the elution box on the detection box, the detection test paper is located between the deflector and the absorbent paper; the bubble is broken, and the washing liquid flows from the bubble along the deflector to the It flows out and reaches the developing area of the test paper for washing; after that, the washing liquid is absorbed by the absorbent paper to prevent the washing liquid from leaking or accumulating in the base and the detection box. In this way, the work of detection and washing is completed.

The structure of the detection test paper is the same as that of the conventional detection test paper, including a sample loading area and a detection display area, and the detection display area includes a control line development area and a detection line development area. The deflector guides the washing liquid in the bubble to the detection display area of the test paper, and the absorbent paper absorbs the washing liquid by siphon action. When in use, put the detection board into the groove of the base, and clamp the washing box on the detection strip, so that the edge of the test paper, the deflector and the edge of the absorbent paper are attached to each other. Take the processed sample and add it to the sample loading area, waiting for the reaction. Then press the washing box button to puncture the bubbles and wait. Afterwards, evenly drop the chromogenic solution in the inspection display area, and read the result after waiting. The detection display area is provided with magnetic detection reagents, such as magnetic nanoparticles modified with antigens. When the sample is added to the sample loading area, the sample reaches the detection display area through the siphon phenomenon and reacts with the magnetic detection reagent. Under the action of the magnetic field of the first magnet and the second magnet, the magnetic nanoparticles concentrate on the first magnet and the second magnet, prolonging the reaction. time to allow the reagents to fully react with the sample.

Preferably, a first magnet installation hole and a second magnet installation hole are provided in the base, the first magnet installation hole closely fits the first magnet, and the second magnet installation hole tightly fits the second magnet.

Preferably, the detection box and the washing box are orthogonal. In this way, only the developing area of the detection box and the washing area of the washing box can be overlapped to complete the washing work. Specifically, the area between the first face cover and the bottom cover of the detection box just accommodates the deflector and the absorbent paper of the washing box, and the area between the deflector and the absorbent paper just detects the test paper, so that the washing box is completed. Detection box matching.

Detection box

The detection box provides detection test paper, the two ends of the detection test paper are located in the box body, and the developing area of the detection test paper is exposed outside the box body. The so-called exposure means that the developing area of the test paper is not blocked by the face cover, but only supported by the bottom cover.

As a preferred solution, the test box includes a first cover, a second cover and a bottom cover, the first cover and the bottom cover fix the first end of the test paper, and the second cover and the bottom cover fix the second end of the test paper ; The developing area of the detection test paper is exposed between the first cover and the second cover, and the distance between the first cover and the second cover is adapted to the washing box. That is to say, the absorbent paper and the deflector of the washing box can be placed between the first cover and the second cover to realize the washing function.

Preferably, the bottom cover of the detection box is provided with a first magnet positioning hole and a second magnet positioning hole, the first magnet positioning hole is in alignment with the development of the control line of the detection test paper, and the second magnet positioning hole is developed with the detection line of the detection test paper at the counterpoint. Among them, the counterpoint refers to

In this way, when the detection box is installed on the base, the magnet is inserted into the corresponding magnet positioning hole, and the positioning between the detection box and the base can be completed. Of course, other positioning methods can also be used to realize the correspondence between the magnet and the control line and the detection line. For example, corresponding marking lines are set on the base and the detection box, and when the marking lines are aligned, the magnet corresponds to the control line and the detection line.

Preferably, the bottom cover and the first surface cover and the second surface cover pass through the connecting column and the assembly column, and the assembly column has an assembly hole, and the connecting column is in one-to-one correspondence with the assembly hole and is plugged in; the connection column is located on the bottom cover, and the assembly The posts are located on the first cover and the second cover; or, the connecting posts are located on the first cover and the second cover, and the assembly posts are located on the bottom cover.

Preferably, a sample loading hole is provided on the first cover, and the sample loading hole corresponds to the sample loading area of the test paper. The sample reaches the sample loading area of the test paper from the sample loading hole.

washing box

The purpose of the wash cartridge is to be able to mate with the test cartridge and release the wash solution. The washing box provides washing liquid, and the washing liquid washes the test paper.

As a preferred scheme, the washing box comprises a first elution bottom cover, a second elution bottom cover and an elution face cover, a deflector is arranged between the elution face cover and the first elution bottom cover; the elution face cover and Absorbent paper is arranged between the second elution bottom covers. The washing liquid in the washing chamber flows out to the deflector, and the deflector guides the washing liquid to the developing place of the test paper. The absorbent paper absorbs the washing liquid at the developing area through the siphon effect, so that the washing liquid can flow at the detection and display area.

Preferably, there is a cavity for containing bubbles between the first elution bottom cover and the elution surface cover, and a button is provided on the first elution bottom cover, and a piercing member capable of piercing the bubbles is provided on the button. The piercing member is a point or blade, or a blunt block. When not working, there is a distance between the piercing piece and the bubble, and the piercing piece does not touch the bubble and will not release the washing liquid; when washing, press the button, and the piercing piece can burst the bubble and release the washing liquid under pressure.

Preferably, the bubbles are located between the first elution bottom cover and the deflector, there is a guide channel between the guide plate and the first elution bottom cover, and the guide channel communicates with the cavity. After the vacuole bursts, the liquid passes through the deflector channel and flows out of the deflector.

Preferably, the deflector includes a built-in part located between the first elution bottom cover and the elution surface cover and an exposed part outside the area of the first elution bottom cover, the flow guide channel is located in the built-in part, and the built-in The area outside the diversion channel at the top is sealed and connected with the first elution bottom cover; the diversion channel has an outlet connected with the exposed part. In this way, all the washing liquid can be confined in the vacuole and the guide channel, preventing the washing liquid from flowing around irregularly.

Preferably, the deflector is a glass fiber board, or a resin board, or a metal board, or a plastic sheet. The deflector is not limited to the above examples as long as it is a sheet that does not absorb water. The sealing method of the deflector and the first elution bottom cover includes but not limited to: setting a sealant between the deflector and the first elution bottom cover; or a sealing gasket.

Preferably, a groove is provided on the first elution bottom plate, the groove forms the top plate and two side plates of the flow guide channel, the flow guide plate forms the bottom plate of the flow guide channel, and the flow guide plate is a flat plate. Alternatively, grooves are provided on the deflector, the grooves of the deflector form the bottom plate and two side plates of the flow guide channel, and the first elution bottom plate forms the top plate of the flow guide channel. Alternatively, the deflector is provided with a first groove, a second groove is formed on the first elution bottom plate, the first groove is aligned with the second groove, and the first groove and the second groove are combined into a guide channel .

The washing box can realize the precise quantification of the washing liquid, guide the washing liquid accurately to the developing area of the test paper, and absorb the washing liquid after washing to avoid the retention of the washing liquid; the whole detection and washing process is convenient and clean.

The beneficial effects of the present invention are:

1. The external magnet is used to slow down the movement of the labeled target pathogen in the detection area of the LFIA strip, thereby prolonging the reaction time.

2. The combination of HRP signal amplification and magnetic area control technology greatly improves the detection sensitivity, and the detection signal visible to the naked eye can be obtained almost at the single cell level.

3. The method enables rapid detection of pathogens, short period, simple operation, no need to use additional equipment, and no need to perform pre-cultivation operations.

4. Set up the washing box to simplify the washing operation. After the washing is completed, no washing liquid will remain.

Description of drawings

Fig. 1 is a schematic front view of the washing box of the present invention.

Fig. 2 is the back schematic diagram of washing box of the present invention

Fig. 3 is a schematic view of the reverse side of the detection board of the present invention.

Fig. 4 is a schematic front view of the detection board of the present invention.

Fig. 5 is an exploded schematic view of the detection board of the present invention.

Fig. 6 is a schematic diagram of the base of the present invention.

Fig. 7 is a schematic diagram of the detection results of the present invention.

In the attached drawings 1: deflector; 2: button; 3: washing chamber; 4: elution surface cover; 5: first elution bottom cover; 6: second elution bottom cover; 7: absorbent paper; 8: Piercing piece; 9: Sample hole; 10: First cover; 11: Second cover; 12: Bottom cover; 13: Test paper; 13A: Test line developing place; 13B: Control line developing place; 14: First magnet positioning hole; 15: second magnet positioning hole; 16: connecting column; 17: assembly column; 18 second magnet mounting hole; 19: first magnet mounting hole;

Detailed ways

A magnetic focusing rapid detection system, characterized in that it includes a base, a detection box and an elution box, the base has a groove matching the detection box, and a first magnet and a second magnet are arranged in the groove; the detection box includes a box body and a detection box Test paper 13, the detection test paper 13 is fixed on the box body, and the developing part of the detection test paper 13 is exposed outside the box body; the elution box is provided with bubbles storing washing liquid, deflector 1 and absorbent paper 7, deflector 1 and absorbent paper 7 The distance between the papers 7 matches the detection test paper 13 ; the first magnet is in alignment with the control line development 13B of the detection test paper 13 , and the second magnet is in alignment with the detection line development 13A of the detection test paper 13 .

When in use, first put the test box into the groove of the base, the bottom surface of the box body of the test box is attached to the base, the developing area of the test paper 13 is exposed outside the box, and the control line and the test line of the test paper 13 are respectively aligned with the first Magnet and the second magnet; add sample to detection test paper 13, after development, cover the elution box on the detection box, detection test paper 13 is located between deflector 1 and water-absorbing paper 7; make vacuole burst, washing liquid from the vacuole Flow out along the deflector 1 and reach the developing area of the test paper 13 for washing; after that, the washing liquid is absorbed by the absorbent paper 7 to prevent the washing liquid from leaking or accumulating in the base and the detection box. In this way, the work of detection and washing is completed.

The structure of the detection test paper 13 is the same as that of the conventional detection test paper 13, including a sample application area and a detection display area, and the detection display area includes a control line development part 13B and a detection line development part 13A. The deflector 1 guides the washing liquid in the bubbles to the detection and display area of the test paper, and the absorbent paper 7 absorbs the washing liquid by siphon action. When in use, the detection board is put into the groove of the base, the washing box is clamped on the detection strip, and the edges of the test paper, the deflector 1 and the absorbent paper 7 are attached to each other. Take the processed sample and add it to the sample loading area, waiting for the reaction. Then press button 2 of the washing box to puncture the bubbles and wait. Afterwards, evenly drop the chromogenic solution in the inspection display area, and read the result after waiting. The detection display area is provided with magnetic detection reagents, such as magnetic nanoparticles modified with antigens. When the sample is added to the sample loading area, the sample reaches the detection display area through the siphon phenomenon and reacts with the magnetic detection reagent. Under the action of the magnetic field of the first magnet and the second magnet, the magnetic nanoparticles concentrate on the first magnet and the second magnet, prolonging the reaction. time to allow the reagents to fully react with the sample.

Preferably, a first magnet installation hole 19 and a second magnet installation hole 18 are provided in the base, the first magnet installation hole 19 tightly fits with the first magnet, and the second magnet installation hole 18 tightly fits with the second magnet.

Preferably, the detection box and the washing box are orthogonal. In this way, only the developing area of the detection box and the washing area of the washing box can be overlapped to complete the washing work. Specifically, the area between the first surface cover 10 and the bottom cover 12 of the test box just accommodates the deflector 1 and the absorbent paper 7 of the washing box, and the area between the deflector 1 and the absorbent paper 7 just detects the test paper 13 In this way, the matching of the washing box with the detection box is completed.

Detection box

The detection box provides detection test paper 13, the two ends of the detection test paper 13 are located in the box body, and the developing area of the detection test paper 13 is exposed outside the box body. The so-called exposure means that the developing area of the detection test paper 13 is not blocked by the surface cover, and is only supported by the bottom cover 12 .

As a preferred solution, the detection box includes a first cover 10, a second cover 11 and a bottom cover 12, the first cover 10 and the bottom cover 12 fix the first end of the test paper 13, the second cover 11 and the bottom cover 12 fix the second end of detection test paper 13; the development zone of detection test paper 13 is exposed between the first face cover 10 and the second face cover 11, and the distance between the first face cover 10 and the second face cover 11 is the same as that of the washing box adaptation. That is to say, the absorbent paper 7 and the deflector 1 of the washing box can be placed between the first cover 10 and the second cover 11 to realize the washing function.

Preferably, the bottom cover 12 of the detection box is provided with a first magnet positioning hole 14 and a second magnet positioning hole 15, the first magnet positioning hole 14 is in alignment with the contrast line development part 13B of the detection test paper 13, and the second magnet positioning hole 15 It is aligned with the detection line development part 13A of the detection test paper 13. In this way, when the detection box is installed on the base, the magnet is inserted into the corresponding magnet positioning hole, and the positioning between the detection box and the base can be completed. Of course, other positioning methods can also be used to realize the correspondence between the magnet and the control line and the detection line. For example, corresponding marking lines are set on the base and the detection box, and when the marking lines are aligned, the magnet corresponds to the control line and the detection line.

Preferably, the bottom cover 12 is connected to the first surface cover 10 and the second surface cover 11 through the connecting column 16 and the assembly column 17. The assembly column 17 has an assembly hole, and the connection column 16 corresponds to the assembly hole one by one and is plugged in. The connecting column 16 is located on the bottom cover 12, the assembly column 17 is located on the first surface cover 10, and the second surface cover 11; or, the connecting column 16 is located on the first surface cover 10, the second surface cover 11, and the assembly column 17 is located on the bottom cover 12 .

Preferably, a sample loading hole 9 is provided on the first cover 10 , and the sample loading hole 9 corresponds to the sample loading area of the detection test paper 13 . The sample reaches the sample loading area of the detection test paper 13 from the sample loading hole 9 .

washing box

The purpose of the wash cartridge is to be able to mate with the test cartridge and release the wash solution. The washing box provides washing liquid, and the washing liquid washes the detection test paper 13 .

As a preferred solution, the washing box includes a first elution bottom cover 5, a second elution bottom cover 6 and an elution surface cover 4, and a deflector 1 is arranged between the elution surface cover 4 and the first elution bottom cover 5 Absorbent paper 7 is set between the elution surface cover 4 and the second elution bottom cover 6 ; The washing liquid in the washing chamber 3 flows out to the deflector 1 , and the deflector 1 guides the washing liquid to the developing place of the test paper 13 . The absorbent paper 7 absorbs the washing liquid at the developing place through siphon action, so that the washing liquid can flow at the detection and display place.

Preferably, there is a cavity with bubbles between the first elution bottom cover 5 and the elution surface cover 4, and a button 2 is arranged on the first elution bottom cover 5, and a puncture hole capable of piercing the bubbles is provided on the button 2. Item 8. Wherein, the vacuole containing chamber is the washing chamber 3 . The piercing member 8 is a point or blade, or a blunt block. When not working, there is a distance between the piercing piece 8 and the bubble, and the piercing piece 8 does not touch the bubble and will not release the washing liquid; when washing, press the button 2, and the piercing piece 8 can rupture the bubble under pressure. Release the wash solution. Preferably, the bubbles are located between the first elution bottom cover 5 and the deflector 1 , there is a guide channel between the guide plate 1 and the first elution bottom cover 5 , and the guide channel communicates with the bubble containing cavity. After the bubble bursts, the liquid flows out from the deflector 1 through the deflector channel.

Preferably, the deflector 1 includes a built-in part located between the first elution bottom cover 5 and the elution surface cover 4 and an exposed part outside the area of the first elution bottom cover 5, and the flow guide channel is located in the built-in The area outside the diversion channel at the inner part is sealed and connected with the first elution bottom cover 5; the diversion channel has an outlet connected to the exposed part. In this way, all the washing liquid can be confined in the vacuole and the guide channel, preventing the washing liquid from flowing around irregularly.

Preferably, the deflector 1 is a glass fiber board, or a resin board, or a metal board, or a plastic sheet. The deflector 1 is not limited to the above examples as long as it is a thin sheet that does not absorb water. The sealing method between the deflector 1 and the first elution bottom cover 5 includes but not limited to: setting a sealant between the deflector 1 and the first elution bottom cover 5 ; or a sealing gasket.

Preferably, grooves are provided on the first elution bottom plate, the grooves form the top plate and two side plates of the flow guide channel, the flow guide plate 1 forms the bottom plate of the flow guide channel, and the flow guide plate 1 is a flat plate. Alternatively, grooves are provided on the deflector 1 , the grooves of the deflector 1 form the bottom plate and two side plates of the flow guide channel, and the first elution bottom plate forms the top plate of the flow guide channel. Alternatively, the deflector 1 is provided with a first groove, a second groove is formed on the first elution bottom plate, the first groove is aligned with the second groove, and the first groove and the second groove are combined into a guide aisle.

A method of using the magnetic focusing fast detection system is introduced.

Take 25g mL of sample and pulverize it, add it to 225mL of liquid medium, mix it homogeneously and incubate at 36±1°C for 5-6h before use. Take about 100 μL of the treated sample and add it into the sample well 9, and wait for 10 minutes. Press the button 28 on the washing box and wait 10 minutes. Remove the washing box, drop 100 μL of chromogenic solution evenly into the viewing window, and read the result after 15 minutes. As shown in Figure 5, when the C and T lines are both colored, it is a positive reaction, and when the C line is colored, it is a negative reaction when the T line does not develop color. If the C line does not develop color, the test fails or the test card fails. After more than 30 minutes The results read are all invalid results.

Claims (9)

1. A magnetic focusing rapid detection system with elution function, characterized in that: it comprises a base, a detection box and a washing box, the base has a groove matched with the detection box, and the first magnet and the second magnet are arranged in the groove; the detection box Including the box body and test paper, the test paper is fixed on the box body, and the developing part of the test paper is exposed outside the box; the washing box is equipped with bubbles storing washing liquid, deflectors and absorbent paper, between the deflector and absorbent paper The distance between them is matched with the detection test paper; the first magnet is in alignment with the development of the control line of the detection test paper, and the second magnet is in alignment with the development of the detection line of the detection test paper. 2. A magnetic focusing rapid detection system with elution function as claimed in claim 1, characterized in that: a first magnet mounting hole and a second magnet mounting hole are arranged in the groove, and the first magnet mounting hole is connected to the first magnet mounting hole. The magnets are closely matched, and the second magnet installation holes are tightly matched with the second magnets. 3. A magnetic focusing fast detection system with elution function as claimed in claim 1, characterized in that: the detection box and the washing box are orthogonal. 4. A kind of magnetic focusing rapid detection system with elution function as claimed in claim 1, is characterized in that: detection box comprises first cover, second cover and bottom cover, and detection test paper is installed on the first cover , in the test paper slot between the second cover and the bottom cover, the middle part of the test strip is exposed, and there is a gap between the first cover and the second cover, which is matched with the gap of the washing box. 5. A magnetic focusing rapid detection system with elution function as claimed in claim 4, characterized in that: the bottom cover and the first surface cover, the second surface cover are connected with the assembly column through the connecting column, and the assembly column has a The assembling holes and the connecting posts correspond to the assembling holes one by one and fit in a plug-in manner; the connecting posts are located on the bottom cover, and the assembling posts are located on the face cover; or, the connecting posts are located on the face cover, and the assembling posts are located on the bottom cover. 6 . The magnetic focusing rapid detection system with elution function as claimed in claim 5 , wherein a sample loading hole is provided on the first cover, and the sample loading hole corresponds to the sample loading area of the detection test paper. 7 . 7. A magnetic focusing rapid detection system with elution function as claimed in claim 1, characterized in that: the washing box comprises a first elution bottom cover, a second elution bottom cover and an elution surface cover, and the elution A deflector is arranged between the surface cover and the second elution bottom cover. 8. A magnetic focusing rapid detection system with elution function according to claim 7, characterized in that: absorbent paper is arranged between the elution surface cover and the second elution bottom cover. 9. A kind of magnetic focusing quick detection system with elution function as claimed in claim 7, is characterized in that: the vacuole containing cavity that vacuole is equipped with is arranged between the first elution bottom cover and elution surface cover, the first The elution bottom cover is provided with a button, and a piercing part is arranged on the button.