CN110317841A - A kind of griseofulvin fermentation production medium - Google Patents
A kind of griseofulvin fermentation production medium Download PDFInfo
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- CN110317841A CN110317841A CN201910648868.5A CN201910648868A CN110317841A CN 110317841 A CN110317841 A CN 110317841A CN 201910648868 A CN201910648868 A CN 201910648868A CN 110317841 A CN110317841 A CN 110317841A
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- griseofulvin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
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Abstract
The invention discloses a kind of griseofulvin fermentation production mediums, are formulated by the raw material of following weight percent: long rice flour 6%, corn flour 5%, tapioca flour 3%, potassium dihydrogen phosphate 0.6%, calcium carbonate 0.4%, sodium nitrate 0.1%, ammonium sulfate 0.1%, ferrous sulfate 0.1%, sodium chloride 0.8%, potassium chloride 0.7%, magnesium sulfate 0.1%, GPE GPE type 0.05%, surplus is water.Fermentation broth viscosity of the present invention reduces, and fermentation unit is stablized in 28000-30000 μ g/ml, fermentation period contraction 45 hours, reduces fermentation energy consumption, reduces cost, high income.
Description
The present invention relates to raw material medicament preparation, in particular to a kind of griseofulvin fermentation production mediums for technical field.
Background technique griseofulvin be antibiotics bulk pharmaceutical chemicals, English name Griseofulvin, Chinese nickname grisein,
Crystallite griseofulvin, English alias Fulcin, Fulcine, Fulvicin, Fungivin, Grifulvin, Grisactin,
Grisefulin, Griseofulvinum, [CAS] 126-07-8, this product are the attritive powder of white or off-white color；This product master
There is good antibacterial action to superficial mycosis such as trichophyta, sporidiole bacteria, Epidermophytons.
Griseofulvin is treated suitable for various tinea diseases, including favus of the scalp, barber's itch, ringworm of the body, jock itch, tinea pedis and onychomycosis.
Currently, the method for preparing griseofulvin, a, griseofulvin fermented and cultured some cycles heat inactivation, sheet frame solid-liquid point
It pelletizes drying from, filter cake mycelium.B, acetone extract obtains griseofulvin acetone extract liquid；C, griseofulvin acetone extract liquid is living
Property carbon decoloring, destainer condensing crystallizing obtain griseofulvin crude product；D, griseofulvin crude product acetone solution, griseofulvin acetone
Lysate activity carbon decoloring, condensing crystallizing obtains griseofulvin recrystallizer to destainer again；E, griseofulvin recrystallizer butanol
Washing, then with ethanol washing, drying, pulverize to obtain griseofulvin bulk pharmaceutical chemicals.
Summary of the invention makes to consume in production process the object of the present invention is to provide a kind of griseofulvin fermentation production medium
Energy is low, it is short to reduce cost, fermentation period, improves fermentation unit, improves production efficiency.
The technical solution adopted by the invention is as follows:
A kind of griseofulvin fermentation production medium, is formulated by the raw material of following weight percent: long rice flour 6%,
Corn flour 5%, tapioca flour 3%, potassium dihydrogen phosphate 0.6%, calcium carbonate 0.4%, sodium nitrate 0.1%, ammonium sulfate 0.1%, sulfuric acid
Ferrous iron 0.1%, sodium chloride 0.8%, potassium chloride 0.7%, magnesium sulfate 0.1%, GPE GPE type 0.05%, surplus is water.
The present invention has the advantages that carbon source ingredient and proportion in 1, adjustment fermentation medium, through production process monitoring data
Show that carbon source proportion is long rice flour 6%, corn flour 5%, tapioca flour 3% can be such that fermentation broth viscosity reduces, and fermentation unit, which is stablized, to exist
28000-30000 μ g/ml fermentation period contraction 45 hours, reduces fermentation energy consumption, reduces cost；
2, compared with comparative formula, adjustment potassium dihydrogen phosphate is 0.6%, and calcium carbonate 0.4%, fermentation liquid pH is reduced by 6.8
To 5.8, sampled to observe under the microscope, strain growth is more significant than the strain growth speed in control test recipe in fermentation liquid
It speeds；
3, gas-liquid mixed effect in fermentor can be improved by suitable GPE type GPE being added, and improve the speed of oxygen in fermentor
Rate is conducive to strain growth and improves yield；
4, this test recipe is in industrialized production application, and filters than control test recipe, high income about 16%.
The invention will be further described below specific embodiment.
A kind of griseofulvin fermentation production medium, is formulated by the raw material of following weight percent: long rice flour 6%,
Corn flour 5%, tapioca flour 3%, potassium dihydrogen phosphate 0.6%, calcium carbonate 0.4%, sodium nitrate 0.1%, ammonium sulfate 0.1%, sulfuric acid
Ferrous iron 0.1%, sodium chloride 0.8%, potassium chloride 0.7%, magnesium sulfate 0.1%, GPE GPE type 0.05%, surplus is water.
It is as follows using above-mentioned culture medium production griseofulvin method:
A, it by above-mentioned culture medium with 121~126 DEG C of fermentor of loading is postponed, maintains sterilize within 30 minutes, is down to 30 to tank temperature
Degree, inoculation, fermentation, condition of culture: initial medium pH=5.8, cultivation temperature: 29 DEG C ± 1 DEG C, air mass flow: 2500~
3600m3/ h, tank pressure: 0.01~0.05MPa is cultivated to 340 hours, heat inactivation uses plate compression in ferment middle feed supplement
For machine by mycelium and separation of fermentative broth, mycelium adds calcium oxide granulation, drying, is packed into extraction column and adds acetone extract；
B, acetone extract obtains griseofulvin acetone extract liquid；
C, griseofulvin acetone extract liquid activity carbon decoloring, destainer condensing crystallizing obtain griseofulvin crude product；
D, knot is concentrated in griseofulvin crude product acetone solution, griseofulvin acetone solution liquid activity carbon decoloring, destainer again
Crystalline substance obtains griseofulvin recrystallizer；
E, griseofulvin recrystallizer butanol wash, then with ethanol washing, drying, pulverize to obtain griseofulvin raw material
Medicine.
Comparative experiments:
Test recipe (used medium of the present invention)
Long rice flour 6%, corn flour 5%, tapioca flour 3%, potassium dihydrogen phosphate 0.6%, calcium carbonate 0.4%, sodium nitrate
0.1%, ammonium sulfate 0.1%, ferrous sulfate 0.1%, sodium chloride 0.8%, potassium chloride 0.7%, magnesium sulfate 0.1%, GPE GPE type
0.05%, surplus is water.
Comparative formula (culture medium used before our company)
Long rice flour 15%, potassium dihydrogen phosphate 0.4%, calcium carbonate 0.6%, sodium nitrate 0.1%, ammonium sulfate 0.1%, sulfuric acid are sub-
Iron 0.1%, sodium chloride 0.8%, potassium chloride 0.7%, magnesium sulfate 0.1%, surplus are water.
By above-mentioned two formulas ingredient, after disinfection, by seed culture fluid be depressed into fermentor culture after culture, mid-term feed supplement,
Inactivation, mycelium and separation of fermentative broth, extraction, decoloration, crystallization, washing, drying, pulverize to obtain griseofulvin raw material at granulation
Medicine.By testing comparison result such as table three times:
1 comparative test table of table
Conclusion (of pressure testing): through test recipe compared with comparative formula, the fermentation liquid filtering velocity in test recipe is significantly improved, mistake
It is higher than comparative formula by about 16% to filter yield, pH is reduced to 5.8 by 6.8 after disappearing, and fermentation titer is stablized in 28000-30000 μ g/ml,
Fermentation period shortens about 45 hours, annual to save 1,500,000 yuan, improves efficiency for enterprise, improves yield, it is close to create economic benefit
10000000 profits.
Claims (1)
1. a kind of griseofulvin fermentation production medium, which is characterized in that be formulated by the raw material of following weight percent: Xian
Rice flour 6%, corn flour 5%, tapioca flour 3%, potassium dihydrogen phosphate 0.6%, calcium carbonate 0.4%, sodium nitrate 0.1%, ammonium sulfate
0.1%, ferrous sulfate 0.1%, sodium chloride 0.8%, potassium chloride 0.7%, magnesium sulfate 0.1%, GPE GPE type 0.05%, residue
Amount is water.
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| CN201910648868.5A CN110317841B (en) | 2019-07-10 | 2019-07-10 | Fermentation production culture medium for griseofulvin |
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| CN110317841A true CN110317841A (en) | 2019-10-11 |
| CN110317841B CN110317841B (en) | 2022-11-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112094874A (en) * | 2020-09-16 | 2020-12-18 | 内蒙古格林特制药有限责任公司 | Culture medium for producing griseofulvin through fermentation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812489A (en) * | 2010-04-29 | 2010-08-25 | 赤峰制药股份有限公司 | Culture medium for producing griseofulvin by fermentation |
| CN103275047A (en) * | 2013-05-23 | 2013-09-04 | 内蒙古格林特制药有限责任公司 | Preparation method of griseofulvin |
| CN106480121A (en) * | 2016-09-27 | 2017-03-08 | 宁夏泰瑞制药股份有限公司 | A kind of culture medium of utilization penicillium patulum producing griseofulvin by fermentation and cultural method |
| CN108641969A (en) * | 2018-06-21 | 2018-10-12 | 赤峰制药股份有限公司 | A kind of griseofulvin production bacterium culture medium |
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2019
- 2019-07-10 CN CN201910648868.5A patent/CN110317841B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812489A (en) * | 2010-04-29 | 2010-08-25 | 赤峰制药股份有限公司 | Culture medium for producing griseofulvin by fermentation |
| CN103275047A (en) * | 2013-05-23 | 2013-09-04 | 内蒙古格林特制药有限责任公司 | Preparation method of griseofulvin |
| CN106480121A (en) * | 2016-09-27 | 2017-03-08 | 宁夏泰瑞制药股份有限公司 | A kind of culture medium of utilization penicillium patulum producing griseofulvin by fermentation and cultural method |
| CN108641969A (en) * | 2018-06-21 | 2018-10-12 | 赤峰制药股份有限公司 | A kind of griseofulvin production bacterium culture medium |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112094874A (en) * | 2020-09-16 | 2020-12-18 | 内蒙古格林特制药有限责任公司 | Culture medium for producing griseofulvin through fermentation |
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Denomination of invention: A culture medium for the fermentation production of griseofulvin Granted publication date: 20221122 Pledgee: Huaxia Bank Co.,Ltd. Chifeng Branch Pledgor: INNER MONGOLIA GLINT PHARMACEUTICAL CO.,LTD. Registration number: Y2024150000049 |