CN110305779A - A kind of air microorganism detection sampling device - Google Patents

Abstract

The invention discloses a kind of air microbe detection sampler and methods, it is characterised in that it includes package cover that air microbe, which detects sampler, exposure head, encapsulation upper shield and exposure head bracket；Wherein the package cover dress cooperates with package cover open end, covers the open end of package cover.Apparatus and method of the present invention can detect multiple-microorganism flcating germ, applied widely；And natural decay rate is introduced, it ensure that the objectivity and authenticity of testing result, can be used as precise Identification and evaluate the foundation of air purity and clean-up effect.

Description

A kind of air microorganism detection sampling device

technical field

The present invention relates to the field of airborne microorganism detection, and more particularly, to an airborne microorganism detection sampling device and a method thereof.

Background technique

There are a large number of beneficial or harmful microorganisms in the air. Some harmful microorganisms often cause human diseases. The transmission route is mostly airborne. When the concentration of microorganisms reaches a certain concentration, it will cause epidemic outbreaks. According to the statistics of the World Health Organization, nearly 17 million people die each year due to various diseases, the most serious of which are respiratory infections. Living in turbid air for a long time will cause harm to the nervous system, respiratory system and immune system of the human body, resulting in decreased immunity and related diseases. At the same time, with the continuous improvement of the quality of life, people pay more attention to the health of their living environment.

The comprehensive investigation and research at this stage show that the main pollutants in indoor air are divided into three categories: (1) Inhalable particles: including dust, smoke and pollen; (2) Microorganisms: including bacteria, fungi and viruses; (3) Harmful volatilization Gases: including ammonia, carbon monoxide, formaldehyde and benzene, etc. The vast majority of human respiratory infectious diseases are caused by indoor microbial aerosol-borne infections, such as the SARS infection in Hong Kong Taoda Garden in 2003, the epidemic of SARS in 2008, and the bird flu in recent years, all of which are indoor microbial aerosol infections. Due to the high degree of professionalism of microbial testing, some testing and evaluation methods are not perfect.

The content of microorganisms in the air is an important indicator to evaluate the air purification degree. At present, there are two main methods for air microbial detection, one is the sedimentation method, and the other is the impact method. The sedimentation method is to place the detection medium plate open, and after exposure for a certain period of time, cultivate and count the colonies. The impingement method captures and counts microorganisms in the airflow in the direction of the airflow. The sedimentation method cannot account for the relationship between air flow and microbial sedimentation. The impact method can only describe the number of microorganisms in the air flow direction. For the air with turbulent flow and turbulent flow, neither of these two methods can give the distribution law of air microorganisms.

SUMMARY OF THE INVENTION

The technical problem to be solved by the present invention is to provide an air microorganism detection and sampling device and method with simple structure, long service life, easy disassembly and assembly, accurate measurement and convenient operation.

In order to solve the above technical problems, the present invention provides an air microorganism detection and sampling device, which is characterized by comprising an encapsulation sleeve, an exposure head, an encapsulation sleeve cover and an exposure head support; wherein, the exposure head includes a spherical head and a middle support , the spoke disk part, one end of the middle bracket is connected with the inner side of the spherical head, the other side of the middle bracket is connected with the inner side of the spoke disk part, and the outer side of the spoke disk part is provided with a thread extension end;

The packaging sleeve includes a spherical closed portion, a hollow cylindrical portion and an open end, wherein the inner diameter of the open end of the packaging sleeve is larger than the inner diameter of the hollow cylindrical portion;

The packaging sleeve is matched with the open end of the packaging sleeve and covers the open end of the packaging sleeve.

A bayonet is arranged on the inner wall between the open end of the packaging sleeve and the hollow cylindrical portion, and the bayonet on the inner wall is matched with the outer edge of the spoke disc portion.

Further, the inner wall of the open end of the packaging sleeve is provided with a thread, and the thread of the inner wall is matched with the thread of the outer edge of the disc portion of the spoke.

Further, the exposed head can be put into the encapsulation sleeve, and fixed in the encapsulation sleeve through the inner wall thread and the outer edge thread of the spoke disc portion, wherein the spherical closed portion of the encapsulation sleeve wraps the spherical head of the exposed head, and There is just a gap between the inner wall of the spherical closed part of the packaging sleeve and the outer side of the spherical head of the exposed head.

Further, the outer wall of the open end of the packaging sleeve is provided with threads, and the packaging sleeve cover has internal threads; wherein, the outer wall threads of the packaging sleeve are matched with the inner threads of the packaging sleeve.

Further, when the exposed head is in use, the extended end of the thread can be matched with a sampling holder with a standard thread.

Further, the air pressure balance hole is opened on the round surface of the package cover, which can be plugged with cotton.

The present invention also provides a kind of air planktonic microorganism detection method, it is characterized in that:

(1) Take the exposed head and put it into the packaging sleeve, cover the packaging sleeve and cover it with dry hot air for sterilization;

(2) Open the package cover and take out the exposure head, keep the spherical closed part of the package cover vertically downward, put in an appropriate amount of agar medium, and then put the exposure head again, so that the agar medium is evenly filled with the spherical head and the exposed head. Cover the gap in the inner wall of the packaging sleeve and cover the packaging sleeve;

(3) The air pressure balance port of the package cover is blocked with cotton, and the spherical closed part of the package cover is kept vertically downward and the open end of the package cover is vertically upward, and high-pressure steam sterilization is performed;

(4) Use aseptic operation to take out the exposed head from the packaging sleeve and fix it at the sampling point. After exposure for a certain period of time, insert the exposed head into the packaging sleeve, and culture according to the testing requirements;

(5) Optical imaging equipment, scanning and counting the colonies on the hemisphere of the packaging sleeve, calculating the colony density of each vector, and analyzing the movement situation of air microorganisms at the sampling point.

Further, it also includes comparing and screening the colony on the surface of the test body with the standard colony, and recording the detected microbial strain types.

Further, the exposure head is put into a packaging sleeve, covered with a packaging sleeve cover, the air pressure balance port of the packaging sleeve cover is blocked with cotton, and sterilized by dry hot air at 150° C. for 1.5 hours.

Further, after the exposure head is exposed at the sampling point for a certain period of time, the exposure head is re-packed into the encapsulation sleeve, and microorganisms are cultured according to different requirements for bacterial detection or fungal detection.

Compared with the prior art, the present invention has the following beneficial effects:

1. The present invention is different from the traditional flat petri dish by designing a three-dimensional spherical detection body, covering the detection medium, exposing it to the detection sampling point for a certain period of time, culturing, and performing surface colony scanning to display different sagittal colony densities.

2. The collection body of the present invention adopts a spherical design, which is more in line with the law of indoor air flow, and allows a larger area of the air to be detected to contact the detection coating in the collection ball, thereby improving the collection rate.

3. The exposure head of the present invention is provided with a spoke disc part, so in the sterilization and microorganism culture process, the normal flow of the air inside and outside the package can be realized under a controlled working environment, and it can also play a role in the fixed exposure. The role of the position of the head in the packaging sleeve.

4. The traditional microbial collection and detection is mainly composed of a petri dish, which cannot achieve multi-collection comparative detection. After the collection head is used for a period of time, a large number of bacteria will accumulate on the surface. When the collection head is used to absorb sample gas from the air, There will be a certain amount of accumulated bacteria into the petri dish, which will affect the detection quality. The microorganism collection and detection device of the invention can not only complete the functions from sterilization to collection and culture detection, but also realize the convenience, low cost and no residue of replacing the culture medium, and improve the accuracy of the detection result.

Description of drawings

Fig. 1 is the structural representation of the present invention;

FIG. 2 is a schematic diagram of the relative position of the exposure head of the present invention loaded into the packaging sleeve;

FIG. 3 is a schematic side view of the installation position of the exposure head of the present invention loaded into the packaging sleeve;

4 is an exemplary top view of the spoke disc portion of the exposed head of the present invention;

FIG. 5 is a schematic diagram of the appearance of the packaging sleeve of the present invention.

Among them, 1-encapsulation sleeve, 2-encapsulation sleeve cover, 3-spherical head, 4-middle bracket, 5-spoke disc part, 6-inner wall bayonet, 7-thread extension, 8-gap, 9- Balance port, 10-spherical closed part, 11-hollow cylindrical part, 12-open end

Detailed ways

The specific embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and embodiments. The following examples are intended to illustrate the present invention, but not to limit the scope of the present invention.

Example 1

As shown in Figure 1, the air microorganism detection and sampling device includes three components: an encapsulation sleeve (1), an exposure head, and an encapsulation sleeve cover. It can be matched with a sampling holder with a standard screw port for testing.

1.1 Package Set

It includes a spherical closed part (10), a hollow cylindrical part (11) and an open end (12), is made of materials with good optical properties, and requires heat resistance at 200°C for 3 hours. The microbial colonies in the culture medium with a thickness of 2 mm on the inner wall of the packaging sleeve can be scanned with an optical imaging device.

The packaging sleeve (1) is a long cylindrical body with a hemispherical bottom; the appearance is also marked with an indicator line for the filling amount of the liquid medium.

The inner wall of the packaging sleeve is 100 mm in depth along the direction from the open end of the cylinder to the spherical closed portion at a depth of 0-30 mm, and the inner diameter of the packaging sleeve is 90 mm after 30 mm.

The outer wall of the open end of the cylinder is threaded and matched with the package cover.

1.2 Package cover

Requires heat resistance to 200°C for 3 hours. The inner thread of the package cover is opened and matched with the package cover. There is a 0.5mm hole in the package cover, which can be plugged with cotton.

1.3 Exposed Head

Requires 200 ℃, 3 hours, including spherical head (3), middle bracket (4), spoke disc (5). The hemispherical head has a cylindrical bracket in the middle. A spoke disc is connected to the bottom of the middle bracket (4). The spoke disc is 10mm thick and 100mm in diameter. , the cylindrical bracket is fixed at the center of the spoke disc and extends 20mm to the other side of the disc, and the extension end (7) of the thread is threaded, which can be connected with the generally processed sampling bracket.

1- Encapsulation sleeve, 2- Encapsulation sleeve cover, 3- Spherical head, 4- Middle bracket, 5- Spoke disc, 6- Inner wall bayonet, 7- Thread extension, 8- Void, 9- Balance port , 10-spherical closed part, 11-hollow cylindrical part, 12-open end

Example 2

The air planktonic microorganism detection method of the present invention comprises:

(1) Sterilization

Put the exposed head into the packaging sleeve (1), add the packaging sleeve cover (2), and seal the air pressure balance port (9) of the cover with cotton. Sterilize with hot dry air at 150°C for 1.5 hours.

After dry heat sterilization and cooling, open the package cover (2), take out the exposed head, put in an appropriate amount of agar medium, put it into the exposed head, add the package cover (2), and add cotton to seal the gas balance port of the package cover (9). Wrap with kraft paper so that the hemisphere end is vertically facing down, and autoclave.

(2) Sampling

Aseptic operation, open the package cover (2), take out the exposure head, and immediately cover the empty package cover (1) with the package cover.

The exposed head is fixed at the sampling point with a sampling bracket and exposed for a certain period of time.

Aseptically open the cover of the packaging sleeve and insert the exposed head into the packaging sleeve (1), being careful not to turn it.

Put on the packaging cover and send it for culture immediately.

(3) Cultivation

According to different requirements for bacterial testing or fungal testing.

(4) Detection

Use optical imaging equipment to scan and count colonies on the hemisphere of the package, calculate the colony density of each vector, and analyze the movement of air microorganisms at the sampling point.

Claims (10)

1. An air microorganism detection sampling device is characterized by comprising a packaging sleeve, an exposure head, a packaging sleeve cover and an exposure head bracket; wherein,

the exposure head comprises a spherical head part, a middle support and a spoke disc part, wherein one end of the middle support is connected with the inner side of the spherical head part, the other side of the middle support is connected with the inner side of the spoke disc part, and the outer side of the spoke disc part is provided with a thread extension end;

the packaging sleeve comprises a spherical closed part, a hollow cylindrical part and an opening end, wherein the inner diameter of the opening end of the packaging sleeve is larger than that of the hollow cylindrical part;

the packaging sleeve is matched with the opening end of the packaging sleeve, and the opening end of the packaging sleeve is covered by the packaging sleeve.

2. The airborne microbe detection and sampling device of claim 1, wherein a bayonet is provided on the inner wall between the open end of the packaging sleeve and the hollow cylindrical portion, and the bayonet on the inner wall is engaged with the outer edge of the spoke disk portion.

3. The airborne microbe detection and sampling device of claim 1 or 2, wherein the exposure head can be placed in the packaging sleeve and fixed in the packaging sleeve through the bayonet of the inner wall and the thread fit of the outer edge of the spoke disk part, wherein the spherical sealing part of the packaging sleeve wraps the spherical head part of the exposure head, and a gap just exists between the inner wall of the spherical sealing part of the packaging sleeve and the outer side of the spherical head part of the exposure head.

4. The airborne microorganism detecting and sampling device as claimed in claim 1, wherein the outer wall of the opening end of the packaging sleeve is provided with screw threads, and the packaging sleeve is covered with the internal screw threads; wherein, encapsulation cover outer wall screw thread and encapsulation cover internal thread cooperation.

5. The airborne microbial detection sampling apparatus of claim 1 wherein said exposure head is adapted to be used with a standard threaded sampling holder having a threaded extension.

6. The air microbe detecting and sampling device as recited in claim 1, wherein the cover of the packaging sleeve has a circular surface with a pressure balancing hole for plugging cotton.

7. A method for detecting airborne microorganisms is characterized in that:

(1) putting the exposed head into a packaging sleeve, covering the packaging sleeve, and sterilizing by dry hot air;

(2) opening the packaging sleeve cover, taking out the exposure head, filling a proper amount of culture medium, then filling the exposure head again, covering the packaging sleeve cover, keeping the spherical sealing part of the packaging sleeve vertically downward and the opening end of the packaging sleeve vertically upward, and performing high-pressure steam sterilization;

(3) taking out the exposed head from the packaging sleeve by using aseptic operation, fixing the exposed head on a sampling point, inserting the exposed head into the packaging sleeve after exposing for a certain time, and culturing according to the detection requirement;

(4) the optical imaging device carries out bacterial colony scanning count to the encapsulation cover hemisphere part, calculates each vector bacterial colony density, and analysis sampling point air microorganism moves the situation.

8. The method of claim 1, further comprising comparing the surface colonies of the test object with standard colonies, screening, and recording the type of microorganism detected.

9. The method of detecting airborne microorganisms of claim 1, wherein the exposure head is placed in a packaging cover, the packaging cover is covered, the air pressure balancing port of the packaging cover is sealed by cotton, and the packaging cover is sterilized by dry hot air at 150 ℃ for 1.5 hours.

10. The method for detecting airborne microorganisms of claim 1, wherein after the exposure head is exposed for a certain period of time at the sampling point, the exposure head is re-installed in the packaging sleeve, and the microorganism culture is performed according to different requirements of bacteria detection or fungus detection.