CN110302091B

CN110302091B - Composition containing nicotinamide adenine dinucleotide for resisting skin aging and preparation method and application thereof

Info

Publication number: CN110302091B
Application number: CN201910706064.6A
Authority: CN
Inventors: 梅杰; 钱军; 张许昌; 方金波; 巫俊峰; 蒋仪
Original assignee: Shenzhen Lantern Science Co ltd
Current assignee: Shenzhen Lantern Science Co ltd
Priority date: 2019-08-01
Filing date: 2019-08-01
Publication date: 2023-02-07
Anticipated expiration: 2039-08-01
Also published as: CN110302091A

Abstract

The invention provides an anti-skin aging composition containing nicotinamide adenine dinucleotide and a preparation method and application thereof, and provides a composition which comprises 2-7 parts by weight of nicotinamide adenine dinucleotide and one or two of 0.25-2 parts by weight of palmitoyl tripeptide-1 and 0.5-2 parts by weight of sodium ascorbyl phosphate. The invention also provides a preparation method of the composition and application of the composition in preparing a composition for resisting skin aging. The composition provided by the invention has outstanding whitening, anti-wrinkle and moisturizing effects, and can effectively delay skin aging.

Description

Nicotinamide adenine dinucleotide-containing anti-skin-aging composition and preparation method and application thereof

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a composition with skin aging delaying and beautifying effects, and a preparation method and application thereof.

Background

The skin is the largest organ of the human body, wraps the surface of the body, directly contacts with the external environment, and has the functions of protecting, excreting, regulating body temperature, sensing external stimulation and the like. The skin is divided into three layers, namely epidermis, dermis and subcutaneous tissue. The epidermis is on the skin surface and can be divided into two parts, namely a stratum corneum and a stratum germinativum. The cells, which are already keratinized, constitute the stratum corneum, which becomes dander after shedding. The germinal layer cells are continuously divided, and can supplement the desquamated stratum corneum. The hair growth layer contains melanocyte, and the generated melanin can prevent ultraviolet ray from damaging internal tissue. The dermis is a dense connective tissue with many elastic fibers and collagen fibers, and is elastic and tough. The thickness of the dermal tissue is closely related to the fibrous tissue and matrix thereof, and to the phenomena of skin compactness, plumpness, laxity and wrinkling, and has recently received increasing attention from cosmetic skin scientists. With the increase of age and the influence of external factors (such as sunlight), the loss and degradation of dermal matrix components such as collagen and elastin in the dermis of human skin become more serious, which causes skin aging, wrinkles, yellow and rough skin and leather change. How to delay skin aging and relieve aging symptoms is a focus of constant attention of the market and people.

The nicotinamide adenine dinucleotide participates in various physiological activities such as cellular substance metabolism, energy synthesis, cell DNA repair and the like, and has an important effect on the immunity of an organism. Under the healthy state, the concentration of nicotinamide adenine dinucleotide in the human body is stable, and the normal functions of all cells are maintained. The concentration of nicotinamide adenine dinucleotide in the body determines the process and extent of cellular senescence, and a decrease in concentration accelerates the process of cellular senescence. Nicotinamide adenine dinucleotide has been included in the catalogue of used cosmetic raw materials (2015 edition), SEQ ID NO. 07360.

The application of nicotinamide adenine dinucleotide for skin anti-aging has appeared in the prior art. According to the Chinese invention patent application, namely 'narrow-distribution particle size emulsion with skin anti-aging effect and a preparation method thereof' (publication No. CN107837204A, publication No. 2018, 3 and 27), the disclosed emulsion contains 2.5-10% of anti-aging composition, 0.5-10% of emulsifier, 5-20% of grease and 65-85% of water; wherein the anti-aging composition consists of five substances of ribose, camphanediol, pinanediol, dimethyl sulfone and nicotinamide adenine dinucleotide in a mass ratio of 1: 0.5-2: 1-3: 0.5-3. The inventors of the present invention considered that only the above-mentioned five substances were combined in the mass ratio to achieve the optimum anti-skin aging effect. In addition, the Chinese patent application for 'a nourishing and moistening repair essence' (publication No. CN108771643A, 2018, 11, 19) discloses a composition comprising 0.1-10 parts by weight of acetylcysteine, 0.1-10 parts by weight of radix stephaniae tetrandrae extract, 0.1-10 parts by weight of crabapple seed oil, 0.1-10 parts by weight of lavender oil and 0.1-10 parts by weight of nicotinamide adenine dinucleotide, and the best healing effect on sensitive skin is achieved.

Although several anti-aging, skin condition improving compositions containing nicotinamide adenine dinucleotide have been presented. However, as a world-wide area of intense research, the need for skin anti-aging is far from being met.

Disclosure of Invention

In view of the defects of the prior art, the invention aims to provide a composition for relieving skin aging symptoms and improving skin conditions. The composition has simple formula and definite effect, and is suitable for preparing various external preparations.

In order to realize the technical effects, the invention adopts the following technical scheme:

a composition comprises 2-7 parts by weight of nicotinamide adenine dinucleotide and one or two selected from 0.25-2 parts by weight of palmitoyl tripeptide-1 and 0.5-2 parts by weight of sodium ascorbyl phosphate.

As a preferred embodiment, the invention provides a composition I, comprising 2-7 parts by weight of nicotinamide adenine dinucleotide, 0.25-2 parts by weight of palmitoyl tripeptide-1 and 0.5-2 parts by weight of sodium ascorbyl phosphate.

Preferably, in the composition I of the present invention, the weight part of nicotinamide adenine dinucleotide is 3 to 5 parts, and more preferably 3 to 4 parts.

Preferably, the weight part of the palmitoyl tripeptide-1 in the composition I of the invention is 0.5 to 2 parts, and more preferably 0.75 to 2 parts.

Preferably, in the composition I, the weight part of the sodium ascorbyl phosphate is 0.75-2 parts, and more preferably 1-1.75 parts.

As a more preferred embodiment, a composition I of the present invention comprises 2 to 4 parts by weight of nicotinamide adenine dinucleotide, 0.5 to 1 part by weight of palmitoyl tripeptide-1 and 0.75 to 1.25 parts by weight of sodium ascorbyl phosphate.

As a further preferred embodiment, a composition I according to the invention comprises 3 to 4 parts by weight of nicotinamide adenine dinucleotide, 0.5 to 1 part by weight of palmitoyl tripeptide-1 and 1 to 1.25 parts by weight of sodium ascorbyl phosphate.

As a particularly preferred embodiment, a composition I according to the invention comprises 3 parts by weight of nicotinamide adenine dinucleotide, 0.75 parts by weight of palmitoyl tripeptide-1 and 1.25 parts by weight of sodium ascorbyl phosphate.

As a preferred embodiment, the invention also provides a composition I, which consists of nicotinamide adenine dinucleotide, palmitoyl tripeptide-1 and sodium ascorbyl phosphate, wherein the weight parts of each component are as described above.

As another preferred embodiment, the present invention also provides a composition II comprising 2 to 7 parts by weight of nicotinamide adenine dinucleotide and 0.25 to 2 parts by weight of palmitoyl tripeptide-1.

Preferably, in the composition II of the present invention, the weight part of nicotinamide adenine dinucleotide is 3 to 5 parts, and more preferably 3 to 4 parts.

Preferably, the weight part of the palmitoyl tripeptide-1 in the composition II of the present invention is 0.5 to 2 parts, and more preferably 0.75 to 2 parts.

As a preferred embodiment, the invention provides a composition II, which consists of nicotinamide adenine dinucleotide and palmitoyl tripeptide-1, and the weight parts of each component are as described above.

As a further preferred embodiment, the present invention provides a composition III comprising 2 to 7 parts by weight of nicotinamide adenine dinucleotide and 0.5 to 2 parts by weight of sodium ascorbyl phosphate.

Preferably, in the composition III of the present invention, the parts by weight of nicotinamide adenine dinucleotide are 3-5 parts, and more preferably 3-4 parts.

Preferably, in the composition III, the weight part of the sodium ascorbyl phosphate is 0.75 to 2 parts, and more preferably 1 to 1.75 parts.

As a preferred embodiment, the invention provides a composition III, which consists of nicotinamide adenine dinucleotide and sodium ascorbyl phosphate, and the weight parts of the components are as described above.

The invention also aims to provide a preparation method of the composition, the composition I, the composition II or the composition III, which comprises the step of uniformly mixing the components according to the mass ratio.

It is also an object of the present invention to provide the use of the above-mentioned composition, composition I, composition II or composition III for the preparation of a composition for combating skin ageing.

An anti-skin aging composition comprises one of the composition I, the composition II and the composition III, and also comprises an auxiliary material which can be accepted by skin external application; wherein the mass percentage of the composition I, the composition II or the composition III is 2.75-11% based on the mass of the anti-skin-aging composition.

Preferably, the mass percentage of the composition I, the composition II or the composition III is 4.25 to 9 percent based on the mass of the composition for resisting skin aging.

More preferably, the mass percentage of the composition I, the composition II or the composition III is 4.75 to 8 percent based on the mass of the composition for resisting skin aging.

Preferably, the composition for resisting skin aging also comprises 1 to 10 percent of cosmetic special effect additive; the beauty specific additive comprises but is not limited to one or more of allantoin, bisabolol, panthenol, aloe extract and dipotassium glycyrrhizinate in any proportion.

Preferably, the anti-skin aging composition is one or more of a water aqua, an emulsion, a cream, an ointment, an oil and a mask.

The invention also aims to provide a preparation method of the composition for resisting skin aging, which comprises the step of preparing any one of the composition I, the composition II and the composition III and the externally-applied acceptable auxiliary materials for skin into a required dosage form according to a conventional method in the field.

The anti-skin aging is anti-wrinkle, whitening and moisturizing.

The term "parts by weight" as used herein refers to the relative amounts of the components, and is not a specific mass unit. The 1 part by weight may be any gram, any kilogram, such as 1g, 3g, 5g, 10g, 100g, 1kg, etc., as the case may be.

Acceptable adjuvants for topical application to the skin described herein include, but are not limited to: solvents such as water, alcohols, etc., humectants such as glycerin, butylene glycol, trehalose, sodium hyaluronate, etc., bases such as liquid paraffin, petrolatum, polydimethylsiloxane, ethylhexyl palmitate, cetearyl alcohol, beeswax, lanolin, stearic acid, etc., thickeners such as xanthan gum, carbomer, hydroxyethylcellulose, etc., emulsifiers such as ceteareth-25, ceteareth-6, tween series, span series, etc., chelating agents such as disodium EDTA, trisodium citrate, etc., antioxidants such as butylated hydroxytoluene, tocopherol acetate, etc., solubilizing agents such as PEG-40 hydrogenated castor oil, etc., preservatives such as p-hydroxyacetophenone, 1, 2-hexanediol, phenoxyethanol, methyl paraben, etc.

The composition provided by the invention specifically comprises a composition I, a composition II and a composition III, and each component is included in catalogue of used cosmetic raw materials (2015 edition), wherein the sequence number of nicotinamide adenine dinucleotide is 07360, the sequence number of palmitoyl tripeptide-1 is 08747, and the sequence number of sodium ascorbyl phosphate is 04100. The composition of the invention has good safety and can be used for a long time.

The composition I, the composition II and the composition III provided by the invention have the definite effect of delaying skin aging, and are specifically shown in the effects of effectively reducing and alleviating wrinkles and enabling the skin color to become light and glossy. Among these, composition I is the most effective.

Detailed Description

The invention is illustrated below with reference to specific examples. It will be understood by those skilled in the art that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention in any way.

The experimental procedures in the following examples are all conventional ones unless otherwise specified. The raw materials, reagent materials and the like used in the following examples are all commercially available products unless otherwise specified. Wherein, the purchase conditions of part of raw materials and reagents are as follows:

nicotinamide adenine dinucleotide (NAD +), purchased from bandhnu bioengineering (shenzhen) limited, specification model: BT01, factory batch number: BT01N118C005, content: 99.3 percent.

Palmitoyl tripeptide-1, available from Shandong Ji peptide Biotech, inc., specification type: 1000mg/Kg, factory batch number: JT20190103, content: 0.1 percent, and the balance of water and polysorbate-20.

Sodium ascorbyl phosphate, available from tesmann, netherlands, specification type: STAY-C50, factory batch number: UE01611080, content: 99.2 percent.

In the following examples and examples, unless otherwise specified, the percentage concentration (%) means the mass percentage concentration (w/w%).

Study example 1Effect of palmitoyl tripeptide-1 and sodium ascorbyl phosphate on the anti-aging Effect of NAD +

Test article：

Nad + stock:

NAD +5%, glycerol 10%, sodium hyaluronate 0.15%, p-hydroxyacetophenone 0.3%, 1, 2-hexanediol 0.5%, and purified water in balance.

The preparation method comprises the following steps: preparing raw materials according to a formula; adding purified water and p-hydroxyacetophenone into a stirring pot, starting to heat to 80-85 ℃, and preserving heat for 30 minutes. Mixing glycerol and sodium hyaluronate, stirring, adding into a stirring pot, and stirring for 15 min under heat preservation. Then the temperature is reduced. Cooling to 45 deg.C, adding 1, 2-hexanediol and NAD +, and stirring. Cooling to 35 deg.C, and filtering with 200 mesh filter screen.

Stock solutions of NAD + and palmitoyl tripeptide-1

And (3) adding palmitoyl tripeptide-1 into the NAD + stock solution to enable the concentration of the palmitoyl tripeptide-1 to be 1%, and uniformly stirring to obtain the NAD + stock solution.

Stock solutions of NAD + and sodium ascorbyl phosphate

And (3) adding sodium ascorbyl phosphate into the NAD + stock solution to enable the concentration of the sodium ascorbyl phosphate to be 1%, and uniformly stirring to obtain the NAD + stock solution.

Stock solutions of NAD + and palmitoyl tripeptide-1, sodium ascorbyl phosphate

And (3) adding palmitoyl tripeptide-1 and sodium ascorbyl phosphate into the NAD + stock solution to ensure that the concentrations of the palmitoyl tripeptide-1 and the sodium ascorbyl phosphate are all 1%, and uniformly stirring to obtain the NAD + stock solution.

The requirements of the tested volunteers：

At least 28 healthy volunteers were selected and randomly and evenly divided into 4 groups.

Selecting a standard: 1. male and female are not limited, and the age is 20-65 years; 2. healthy, without any history of skin or systemic disease; 3. the test is matched, the attitude is serious, certain expression capability is realized, and the feeling after use can be truly reflected; 4. voluntarily participates in the study, signs an informed consent, can strictly comply with the requirements of the study scheme, uses the product according to the requirements of the study scheme and completes follow-up visits.

Exclusion criteria: 1. pregnant or lactating women; 2. sensitive skin; 3. skin damage or skin diseases affecting the facial state of the person; 4. facial use of laser, chemical exfoliation, botulinum toxin, dermal augmentation, or skin pulling; 5. sunburn occurs in nearly 1 month; 6. other facial clinical studies or dermatologist patients have been enrolled currently or within approximately 3 months.

Termination and rejection criteria: 1. volunteers asked to terminate the test; 2. the volunteer has poor compliance; 3. the test can not be continued due to adverse reaction or special physiological and pathological changes and the like; 4. other skin care products that had an effect on this test were used in the test; 5. excessive ultraviolet irradiation was received.

Testing procedure：

The tested part is unified as the facial skin.

The subject first cleanses the area of the subject with warm water and then blots the excess water from the face with a dry non-dandruff absorbent paper towel. Sit still for 20min at room temperature (20 +/-2 ℃) and relative humidity of 50% +/-5%. 2.0g +/-0.02 g of test substance is taken according to groups and evenly smeared on the facial skin of a test subject. The test article is administered once a day, in the morning and evening. The application is continued for 30 days. On day 31 volunteers scored the sensory and sensory evaluation of the test subjects, and the average value of each group was recorded and calculated.

Scoring criteria：

Adopting a scoring standard of 1-100 grades:

1-20 minutes, the effect is worse after use; 21-40 points, no effect; 41 to 60 minutes, and the effect is general; 61-80 minutes, the effect is obvious; 81 to 100 minutes, the effect is very remarkable.

As a result, the：

All tests were completed by each group of subjects, and no termination or culling occurred. The average values of the scores of the individual groups are shown in table 1.

TABLE 1 Effect of palmitoyl tripeptide-1 and sodium ascorbyl phosphate on NAD + anti-aging Effect

Sample of test substance	Anti-wrinkle effect	Whitening effect	Water replenishing effect	Mean score
					NAD + stock solution	78	71	80	76.33
NAD + and palmitoyl tripeptide-1	88	76	86	83.33
					NAD + and ascorbic acidSodium phosphate ester of folic acid	79	85	88	84.00
NAD +, palmitoyl tripeptide-1 and sodium ascorbyl phosphate	92	88	90	90.00

From the above table, it can be seen that:

1) Compared with the single use of NAD +, the palmitoyl tripeptide-1 can obviously enhance the anti-wrinkle effect of NAD +. The palmitoyl tripeptide-1 serving as a signal transmission small molecular polypeptide can obviously promote the synthesis of skin collagen, fibronectin and hyaluronic acid, can obviously reduce the wrinkle depth, density and average volume, and can improve the skin quality and roughness of the skin.

2) The sodium ascorbyl phosphate can obviously enhance the whitening and repairing effects of the NAD +. Sodium ascorbyl phosphate as a reducing agent can effectively remove free radicals in the skin and reduce the damage of the free radicals to the skin, thereby enhancing the anti-aging effect of NAD +.

3) Among all the subjects, palmitoyl tripeptide-1 in combination with sodium ascorbyl phosphate had the best effect of enhancing NAD + anti-aging.

Conclusion: the palmitoyl tripeptide-1 and the sodium ascorbyl phosphate can respectively enhance the skin aging resisting effect of NAD +, and the combination of the palmitoyl tripeptide-1 and the sodium ascorbyl phosphate has the best effect.

Study example 2Optimization of ingredient distribution ratio in the composition provided by the invention

(ii) optimization of NAD + dosage

The test substance:

according to the method for preparing the NAD + stock solution described in research example 1, a series of NAD + solutions were prepared, with concentrations of NAD + of 0%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, and 8%, respectively.

Volunteer subjects:

at least 45 healthy volunteers were selected and randomly and evenly divided into 9 groups.

Selection criteria, exclusion criteria, termination and rejection criteria: the same as in "research example 1".

Test procedure：

The same as in "research example 1".

Scoring criteria：

The same as in "research example 1".

As a result, the：

All tests were completed by each group of subjects, and no termination or culling occurred. The average values of the scores of the individual groups are shown in Table 2.

TABLE 2 Effect of NAD + dosage on skin anti-aging Effect

NAD + concentration (%)	Anti-wrinkle effect	Whitening effect	Water replenishing effect	Mean score
					0	40	35	45	40.00
1	60	55	65	60.00
					2	68	66	76	70.00
3	78	71	80	76.33
					4	78	71	80	76.33
5	78	71	80	76.33
					6	78	70	75	74.33
7	78	65	70	71.00
					8	78	63	30	57.00

As can be seen from Table 2, when the concentration of NAD + is 2% to 7%, the anti-aging effect is significant, and when the concentration exceeds 7%, the repairing effect is sharply reduced. When the concentration of NAD + is 3% -5%, the using effect of each item of skin reaches the peak value, and the score of each effect cannot be increased by increasing the using amount of NAD +. Thus, NAD + is preferably present in a concentration range of 2% to 4% or 2% to 5%, more preferably in a concentration range of 3% to 4% or 3% to 5%; considering that NAD + is expensive, the optimal concentration of NAD + is 3%.

Optimization of dosage of (di) palmitoyl tripeptide-1

The test substance:

according to the preparation method of research example 1, a stock solution with a NAD + concentration of 3% was prepared, and palmitoyl tripeptide-1 was added to 3% NAD + stock solution in a certain ratio to prepare a series of solutions.

Subject:

Test procedure：

The same as in "research example 1".

Scoring criteria：

The same as in "research example 1".

Results：

All tests were completed by each group of subjects, and no termination or culling occurred. The average values of the scores of the individual groups are shown in Table 3.

TABLE 3 Effect of palmitoyl tripeptide-1 addition on skin use Effect

Amount of palmitoyl tripeptide-1 added (%)	Anti-wrinkle effect	Whitening effect	Water replenishing effect	Mean score
					0	78	71	80	76.33
0.25	82	73	83	79.33
					0.50	85	75	85	81.67
0.75	88	76	86	83.33
					1.00	88	76	86	83.33
1.25	88	76	86	83.33
					1.50	88	76	86	83.33
1.75	89	76	86	83.67
					2.00	89	76	86	83.67

As shown in Table 3, when the addition amount of palmitoyl tripeptide-1 is 0.25%, the anti-aging effect of NAD + can be enhanced; when the content of the palmitoyl tripeptide is 0.75%, the using effect of each item of skin basically reaches the peak value, and the addition amount of the palmitoyl tripeptide-1 is increased, so that the effect scores can be still increased slightly. Thus, the preferred concentration range for palmitoyl tripeptide-1 is 0.25% to 2%, in which concentration ranges 0.5% to 2%, 0.5% to 1%, and 0.75% to 2% are more preferred amounts; considering that palmitoyl tripeptide-1 is expensive, the optimal amount of palmitoyl tripeptide-1 is temporarily 0.75% when 3% of NAD + is used.

Optimization of dosage of sodium ascorbyl phosphate

The test substance:

according to the preparation method of research example 1, a stock solution with a NAD + concentration of 3% was prepared, and sodium ascorbyl phosphate was added to the stock solution at a ratio of 3% by weight of NAD + to prepare a series of solutions.

Subject:

Selection criteria, exclusion criteria and termination and rejection criteria: the same as in "research example 1".

Test procedure：

The same as in "research example 1".

Scoring criteria：

The same as in "research example 1".

Results：

All tests were completed by each group of subjects, and no termination or culling occurred. The average values of the scores of the individual groups are shown in Table 4.

TABLE 4 Effect of sodium ascorbyl phosphate addition on skin application Effect

Addition amount of sodium ascorbyl phosphate (%)	Anti-wrinkle effect	Whitening effect	Water replenishing effect	Mean score
					0	78	71	80	76.33
0.25	78	73	82	77.67
					0.50	78	76	85	79.67
0.75	78	80	87	81.67
					1.00	79	85	88	84.00
1.25	79	85	87	83.67
					1.50	79	85	87	83.67
1.75	78	85	86	83.00
					2.00	78	85	82	81.67

As can be seen from Table 4, when the addition amount of sodium ascorbyl phosphate is 0.5%, the anti-aging effect of NAD + can be enhanced relatively remarkably; when the content of the sodium ascorbate sodium phosphate is 1.0%, the using effect of each item of skin reaches the peak value, the addition amount of the sodium ascorbate sodium phosphate is increased, and the average score of each effect is basically unchanged. When the dosage of the sodium ascorbyl phosphate reaches 2%, the using effect of the skin is reduced to the peak value, but the dosage is the same as that of the sodium ascorbyl phosphate when the dosage is 0.75%. Therefore, the preferable using amount range of the sodium ascorbyl phosphate is 0.5 to 2 percent; wherein, 0.75% -2% and 0.75% -1.25% are further preferable dosage ranges, more preferably 1% -1.25% or 1% -1.75%; considering that sodium ascorbyl phosphate is expensive, when the amount of NAD + is 3%, the optimum amount of sodium ascorbyl phosphate is temporarily 1.0%.

Study example 3The proportion of the dosage of each component in the composition is optimized

Orthogonal experiments are utilized to optimize the dosage proportion of NAD +, palmitoyl tripeptide-1 and sodium ascorbyl phosphate in the composition.

The weight percentage contents of NAD + (A), palmitoyl tripeptide-1 (B) and sodium ascorbyl phosphate (C) are selected as factors of an orthogonal test, the evaluation standard is the average score of the use effect, and the factor level table is shown in the following table 5. Each test substance was prepared according to the method of study example 1, then test volunteers (45 persons), groups (5 persons/group) were selected and tested according to the method of study example 1, and finally, the test volunteers were scored according to the scoring criteria of study 1. The average score is used as the result of the orthogonal test, which is shown in table 6.

TABLE 5 factor level table

	A(NAD+)(％)	B (palmitoyl tripeptide-1) (%)	C (sodium ascorbyl phosphate) (%)
				1	2	0.5	0.75
2	3	0.75	1.00
				3	4	1.00	1.25

TABLE 6 results of orthogonal experiments

In table 6, I is the sum of the composite scores at the first level corresponding to each factor, II is the sum of the composite scores at the second level corresponding to each factor, III is the sum of the composite scores at the third level corresponding to each factor, I/3 is the average of the composite scores at the first level corresponding to each factor, II/3 is the average of the composite scores at the second level corresponding to each factor, and III/3 is the average of the composite scores at the third level corresponding to each factor. R is the range of the respective factors.

As can be seen from table 6, the composite score corresponding to experiment number 5 is the highest and reaches 92 points, the composite score corresponding to experiment number 9 is the second, the composite score is 91 points, and the composite scores corresponding to experiment numbers 4 and 8 are both 90 points. These scores were all higher than the highest score in study example 2, indicating that the three ingredients produced significant synergy at the specified dose ratios.

The conditions of the above four experiments are shown in table 7 below.

TABLE 7 visual analysis chart of orthogonal test

Test No	NAD+(％)	Palmitoyl tripeptide-1 (%)	Ascorbic acid sodium phosphate (%)	Composite score
					5	3	0.75	1.25	92.00
9	4	1.00	1.00	91.00
					4	3	0.50	1.00	90.00
8	4	0.75	0.75	90.00

From range analysis, the factors that have the greatest influence on the composite score are: a (NAD +) > C (sodium ascorbyl phosphate) > B (palmitoyl tripeptide-1). The condition of highest composite score is A ₂ B ₂ C ₃ That is, the amount of NAD + is 3%, the amount of palmitoyl tripeptide-1 is 0.75%, and the amount of sodium ascorbyl phosphate is 1.25%.

The conditions with the highest composite score were subjected to 3 experimental verifications, and the results are shown in table 8 below.

TABLE 8 verifiability test

Test No	NAD+(％)	Palmitoyl tripeptide-1 (%)	Ascorbic acid phosphate sodium (%)	Composite scoring
					10	3	0.75	1.25	93
11	3	0.75	1.25	93
					12	3	0.75	1.25	93

The verification test result shows that: the combination of NAD +3%, palmitoyl tripeptide-1.75% and sodium ascorbyl phosphate 1.25% has the highest comprehensive score on trial use, and is the most preferred combination in the invention.

Example 1Composition I and solution containing same

The composition of composition I (500 g) described in this example is:

NAD +300g, palmitoyl tripeptide-1 75g, and sodium ascorbyl phosphate 125g

NAD +300g, palmitoyl tripeptide-1 75g, sodium ascorbyl phosphate 125g and purified water 500g are weighed. Mixing the four materials, and stirring uniformly to obtain a target solution; the storage temperature is-15 to-20 ℃ to prevent the growth of microorganisms.

Example 2Anti-aging toning lotion

The anti-aging astringent (1000 g) of the embodiment comprises the following raw materials: 100g of the solution containing the composition I prepared in example 1, 50g of glycerol, 30g of butanediol, 0.2g of EDTA disodium, 1g of allantoin, 5g of trehalose, 50g of an aloe vera extract, 3g of p-hydroxyacetophenone, 5g of 1, 2-hexanediol, 0.6g of PEG-40 hydrogenated castor oil, 0.2g of essence and 755g of purified water.

The preparation method comprises the following steps: the raw materials are prepared according to the formula. Adding purified water, glycerin, butanediol, EDTA disodium, allantoin, trehalose and p-hydroxyacetophenone into a stirring pot, heating to 80-85 ℃, and preserving heat for 30 minutes. Cooling to 45 deg.C, and adding mixed solution of PEG-40 hydrogenated castor oil and essence. Cooling to 35 deg.C, adding the solution containing composition I of example 1, aloe Barbadensis Miller extractive solution, and 1, 2-hexanediol, and stirring. Filtering with 200 mesh filter screen.

In addition, 1000g of a general lotion not containing the composition I described in example 1 was prepared according to the above preparation method. The volunteer subjects were then tested and scored according to the method of study example 1.

Volunteer subjects:

healthy volunteers were selected 40 and randomly and evenly divided into 2 groups.

Testing procedure：

The same as in "research example 1".

Scoring criteria：

The same as in "research example 1".

Results：

All tests were completed by each group of subjects, and no termination or culling occurred. The average values of the scores of the groups are shown in Table 9.

TABLE 9 comparison of use effects of the lotion of example 2 with that of the general lotion

	Water replenishing effect	Anti-wrinkle effect	Whitening effect	Composite scoring
					Toning lotion of the embodiment	95	95	91	94
Common toning lotion	58	32	30	43.75

As can be seen from Table 9, the use effects of the lotions containing the composition I of the present invention are far superior to those of the ordinary lotions not containing the composition I of the present invention.

Example 3Anti-aging stock solution

The anti-aging stock solution (1000 g) described in this example comprises the following raw materials: 100g of the composition I-containing solution prepared in example 1, 80g of glycerin, 30g of butanediol, 1.5g of sodium hyaluronate, 0.2g of disodium EDTA, 1g of allantoin, 10g of trehalose, 100g of aloe vera extract, 3g of p-hydroxyacetophenone, 5g of 1, 2-hexanediol, 0.6g of PEG-40 hydrogenated castor oil, 0.2g of essence, and 668.5g of purified water.

The preparation method comprises the following steps: the raw materials are prepared according to the formula. Adding purified water, glycerin, EDTA disodium, allantoin, trehalose and p-hydroxyacetophenone into a stirring pot, uniformly mixing butanediol and sodium hyaluronate, adding into the stirring pot, heating to 80-85 ℃, and preserving heat for 30 minutes. Cooling to 45 deg.C, and adding mixed solution of PEG-40 hydrogenated castor oil and essence. The temperature is reduced to 35 ℃, the solution containing the composition I prepared in the example 1, the aloe vera extract and the 1, 2-hexanediol are added and stirred evenly. Filtering with 200 mesh filter screen.

In addition, 1000g of a conventional stock solution without the composition I described in example 1 was prepared according to the above preparation method. The volunteer subjects were then tested and scored according to the method of study example 1.

Volunteer subjects:

healthy volunteers were selected for 40 and randomly and evenly divided into 2 groups.

Testing procedure：

The same as in "research example 1".

Scoring criteria：

The same as in "research example 1".

As a result, the：

All tests were completed by each group of subjects, and no termination or culling occurred. The average values of the scores of the groups are shown in Table 10.

TABLE 10 comparison of the use of the anti-aging stock solution of example 3 with that of a conventional stock solution

	Water replenishing effect	Anti-wrinkle effect	Whitening effect	Composite score
					Stock solution of example 3	95	95	91	94
Common stock solution	58	32	30	43.75

As can be seen from Table 10, the use effect of the stock solution containing the composition I of the present invention is far better than that of the ordinary stock solution not containing the composition I of the present invention.

Example 4Anti-aging emulsion

The anti-aging emulsion (1000 g) described in this example comprises the following raw materials: 100g of the solution containing the composition I prepared in example 1, 50g of glycerol, 30g of butanediol, 2g of xanthan gum, 0.2g of disodium EDTA, 1g of allantoin, 4g of p-hydroxyacetophenone, 25 15g of ceteareth-25, 6 15g of ceteareth-6, 30g of cetearyl alcohol, 30g of liquid paraffin, 10g of petrolatum, 0.5g of butylated hydroxytoluene, 10g of polydimethylsiloxane, 10g of ethylhexyl palmitate, 1g of bisabolol, 5g of tocopherol acetate, 5g of 1, 2-hexanediol, 1g of essence, and 680.3g of purified water.

The preparation method comprises the following steps: the raw materials are prepared according to the formula. Adding purified water, EDTA disodium, allantoin, p-hydroxyacetophenone and cetostearyl polyether-25 into a water phase pot, and starting to heat to 80-85 ℃. The glycerol, the butanediol and the xanthan gum are stirred and dispersed uniformly in advance by a container and then are added into a water phase pot. The water phase pot is stirred for 30 minutes under heat preservation. Adding ceteareth-6, cetearyl alcohol, liquid paraffin, petrolatum, butylated hydroxytoluene, polydimethylsiloxane and ethylhexyl palmitate into an oil phase pot, starting to heat to 80-85 ℃, and stirring until the oil phase materials are completely dissolved. Firstly, adding the water phase material into an emulsifying pot, then adding the oil phase material into the emulsifying pot, starting homogenizing for 5 minutes, stirring for 10 minutes, and then, starting to cool. Cooling to 45 deg.C, adding bisabolol, tocopheryl acetate and essence, and stirring. The temperature was reduced to 35 ℃ and the solution containing composition I prepared in example 1 and 1, 2-hexanediol were added and stirred well. Filtering with 200 mesh filter screen.

In addition, 1000g of a conventional emulsion without the composition I described in example 1 was prepared according to the above preparation method. Volunteer subjects were then tested and scored as per study example 1.

Volunteer subjects:

Test procedure：

The same as in "research example 1".

Scoring criteria：

The same as in "research example 1".

Results：

All tests were completed by each group of subjects, and no termination or culling occurred. The average values of the scores of the respective groups are shown in Table 11.

TABLE 11 comparison of the use of the anti-aging emulsion of example 4 with conventional emulsions

	Water replenishing effect	Anti-wrinkle effect	Whitening effect	Mean score
					Emulsion of example 4	96	95	92	94.75
Ordinary emulsion	78	38	35	54

As can be seen from Table 11, the emulsions containing composition I of the present invention are far superior in each use effect to the conventional emulsions not containing composition I of the present invention.

Example 5Anti-aging face cream

The anti-aging cream (1000 g) described in this embodiment comprises the following raw materials: 100g of the solution containing composition I prepared in example 1, 50g of glycerol, 50g of butanediol, 0.3g of disodium EDTA, 1g of allantoin, 4g of p-hydroxyacetophenone, 2.5g of carbomer, 25 15g of ceteareth-6, 50g of cetearyl alcohol, 40g of liquid paraffin, 30g of petrolatum, 20g of polydimethylsiloxane, 20g of ethylhexyl palmitate, 20g of beeswax, 0.5g of butylated hydroxytoluene, 2g of triethanolamine, 1g of bisabolol, 5g of tocopherol acetate, 5g of 1, 2-hexanediol, 1.5g of a perfume compound, and 567.2g of purified water.

The preparation method comprises the following steps: the raw materials are prepared according to the formula. Adding purified water, glycerin, butanediol, EDTA disodium, allantoin, p-hydroxyacetophenone and cetostearyl polyether-25 into a water phase pot, starting to heat to 80-85 ℃, and preserving heat for 30 minutes. Adding ceteareth-6, cetearyl alcohol, petrolatum, polydimethylsiloxane, ethylhexyl palmitate, beeswax and butylated hydroxytoluene into an oil phase pot, heating to 80-85 ℃, and stirring until the oil phase materials are completely dissolved. Firstly, adding the water phase material into an emulsifying pot, and then adding the oil phase material into the emulsifying pot. Stirring and dispersing liquid paraffin and carbomer uniformly in a container in advance, adding into an emulsifying pot, and stirring uniformly. The homogenization is started for 5 minutes, and after stirring for 10 minutes, the temperature is reduced. Cooling to 65 deg.C, adding triethanolamine, and stirring. Cooling to 45 deg.C, adding bisabolol, tocopheryl acetate and essence, and stirring. The temperature was reduced to 35 ℃ and the solution containing composition I prepared in example 1 and 1, 2-hexanediol were added and stirred well. Filtering with 200 mesh filter screen.

In addition, 1000g of a normal cream without the composition I described in example 1 was prepared according to the above preparation method. Volunteer subjects were then tested and scored as per study example 1.

Volunteer subjects:

36 healthy volunteers were selected and randomly and evenly divided into 2 groups.

Test procedure：

The same as in "research example 1".

Scoring criteria：

The same as in "research example 1".

Results：

All tests were completed by each group of subjects, and no termination or culling occurred. The average values of the scores of the individual groups are shown in Table 12.

TABLE 12 comparison of the use of the cream of example 5 with that of a conventional cream

	Water replenishing effect	Anti-wrinkle effect	Whitening effect	Mean score
					Cream of example 5	96	95	93	95
Common face cream	83	39	32	55.5

As can be seen from Table 12, the cream containing composition I of the present invention has far better using effect than the common cream without the composition I.

Example 6Anti-aging eye essence

The anti-aging eye essence (1000 g) in the embodiment comprises the following raw materials: 100g of solution containing the composition I prepared in example 1, 50g of glycerol, 50g of butanediol, 0.3g of EDTA disodium, 5g of carbomer, 4g of triethanolamine, 1g of allantoin, 50g of glycerol polymethacrylate, 5g of beta-glucan, 20g of hydrolyzed collagen, 20g of glycosaminoglycans, 4g of p-hydroxyacetophenone, 5g of 1, 2-hexanediol, 0.2g of essence, 0.6g of PEG-40 hydrogenated castor oil and 684.9g of purified water.

The preparation method comprises the following steps: the raw materials are prepared according to the formula. Adding purified water into a stirring pot, then spreading carbomer on the water surface of the stirring pot, and standing until the carbomer is completely wetted by the water. Adding glycerin, butanediol, EDTA disodium, allantoin and p-hydroxyacetophenone into a stirring pot, heating to 80-85 ℃, and preserving heat for 30 minutes. Cooling to 65 deg.C, adding triethanolamine, and stirring. Cooling to 55 deg.C, adding glycerol polymethacrylate, beta-dextran, hydrolyzed collagen, and glucosamino dextran, and stirring. Cooling to 45 deg.C, adding mixed solution of PEG-40 hydrogenated castor oil and essence, and stirring. The temperature was reduced to 35 ℃ and the solution containing composition I prepared in example 1 and 1, 2-hexanediol were added and stirred well. Filtering with 200 mesh filter screen.

In addition, 1000g of a general eye essence not containing the composition I described in example 1 was prepared according to the above preparation method. Then, volunteers (44 individuals in total, randomly and evenly divided into 2 groups; selection criteria, exclusion criteria and rejection termination criteria were the same as in "example 1") were tested and scored according to the method of example 1, but the volunteers applied the eye with 0.1g of eye essence evenly around the eyes twice in the morning and at night.

The average of the volunteer ratings was calculated and recorded as shown in table 13.

TABLE 13 eye essence of example 6 comparison with general eye essence

	Water replenishing effect	Anti-wrinkle effect	Whitening effect	Mean score
					The invention relates to a medicine for treating eye essence	96	95	93	95
General eye essence	79	71	40	66.25

As can be seen from table 13, the eye essence containing the composition I of the present invention has far better use effects than the common eye essence not containing the composition I of the present invention.

Example 7Anti-aging face mask

The anti-aging mask comprises mask essence and mask base cloth. The facial mask essence comprises the following raw materials: 100g of the composition I-containing solution prepared in example 1, 50g of glycerin, 30g of butylene glycol, 0.2g of disodium EDTA, 1g of allantoin, 1.5g of dipotassium glycyrrhizinate, 5g of trehalose, 3g of panthenol, 5g of hydroxyethyl cellulose, 3g of p-hydroxyacetophenone, 5g of 1, 2-hexanediol, 0.3g of PEG-40 hydrogenated castor oil, 0.1g of essence, and 795.9g of purified water.

The preparation method comprises the following steps: preparing raw materials of the facial mask essence according to a formula; adding purified water into a stirring pot, then adding hydroxyethyl cellulose into the stirring pot, and uniformly stirring. Then starting the furnace and raising the temperature to 80-85 ℃. Adding glycerol, butanediol, EDTA disodium, allantoin and p-hydroxyacetophenone into a stirring pot, and keeping the temperature for 30 minutes. Cooling to 55 deg.C, adding dipotassium glycyrrhizinate, trehalose, and panthenol, and stirring. Cooling to 45 deg.C, adding mixed solution of PEG-40 hydrogenated castor oil and essence, and stirring. The temperature was reduced to 35 ℃ and the solution containing composition I prepared in example 1 and 1, 2-hexanediol were added and stirred well. Filtering with 200 mesh filter screen to obtain facial mask essence. And (3) pouring the facial mask essence into a facial mask bag which is pre-filled with facial mask base cloth through a grouting machine, and sealing.

In addition, 1000g of a general facial mask not containing the composition I described in example 1 was prepared according to the above preparation method. Volunteer subjects (total 28 persons, randomized and evenly divided into 2 groups) were then tested and scored as in study example 1, except that subjects applied the tested mask to the face for 20 minutes every night for 7 days.

The average of the volunteer ratings was calculated and recorded as shown in table 14.

TABLE 14 comparison of the use effects of the top film of example 7 with that of a conventional top film

	Water replenishing effect	Anti-wrinkle effect	Whitening effect	Mean score
					Example 7 face mask	96	96	95	95.75
Common face mask	88	58	49	68.25

As can be seen from Table 14, the effect of the facial mask containing the composition I of the present invention is far better than that of the ordinary facial mask without the composition I of the present invention.

Example 8Composition II and solution containing same

Composition II (400 g) described in this example had the composition:

NAD +300g and palmitoyl tripeptide-1 100g

NAD +300g, palmitoyl tripeptide-1 100g and purified water 600g are weighed. Mixing the three solutions, and stirring to obtain target solution; the storage temperature is-15 to-20 ℃ to prevent the growth of microorganisms.

Example 9Anti-aging face cream

The anti-aging cream (1000 g) described in this embodiment comprises the following raw materials: 100g of the solution containing composition II prepared in example 8, 50g of glycerol, 50g of butanediol, 0.3g of disodium EDTA, 1g of allantoin, 4g of p-hydroxyacetophenone, 2.5g of carbomer, 25-15 g of ceteareth-25, 6-15 g of ceteareth-6, 50g of cetearyl alcohol, 40g of liquid paraffin, 30g of petrolatum, 20g of polydimethylsiloxane, 20g of ethylhexyl palmitate, 20g of beeswax, 0.5g of butylated hydroxytoluene, 2g of triethanolamine, 1g of bisabolol, 5g of tocopherol acetate, 5g of 1, 2-hexanediol, 1.5g of an essence, and 567.2g of purified water.

The preparation method comprises the following steps: the raw materials are prepared according to the formula. Adding purified water, glycerin, butanediol, EDTA disodium, allantoin, p-hydroxyacetophenone and cetostearyl polyether-25 into a water phase pot, starting to heat to 80-85 ℃, and preserving heat for 30 minutes. Adding ceteareth-6, cetearyl alcohol, petrolatum, polydimethylsiloxane, ethylhexyl palmitate, beeswax and butylated hydroxytoluene into an oil phase pot, heating to 80-85 ℃, and stirring until the oil phase materials are completely dissolved. Firstly, adding the water phase material into an emulsifying pot, and then adding the oil phase material into the emulsifying pot. Stirring and dispersing liquid paraffin and carbomer uniformly in a container in advance, adding into an emulsifying pot, and stirring uniformly. The homogenization is started for 5 minutes, and after stirring for 10 minutes, the temperature is reduced. Cooling to 65 deg.C, adding triethanolamine, and stirring. Cooling to 45 deg.C, adding bisabolol, tocopheryl acetate and essence, and stirring. The temperature was reduced to 35 ℃ and 100g of the solution containing composition II prepared in example 8 and 1, 2-hexanediol were added and stirred well. Filtering with 200 mesh filter screen.

Example 10Composition III and solution containing same

The composition of composition III (400 g) described in this example was:

NAD +300g and sodium ascorbyl phosphate 100g

NAD +300g, sodium ascorbyl phosphate 100g and purified water 600g were weighed. Mixing the three solutions, and stirring to obtain target solution; the storage temperature is-15 to-20 ℃ to prevent the growth of microorganisms.

Example 11Anti-aging emulsion

The anti-aging emulsion (1000 g) described in this example comprises the following raw materials: 100g of the solution containing composition III prepared in example 10, 50g of glycerin, 30g of butanediol, 2g of xanthan gum, 0.2g of disodium EDTA, 1g of allantoin, 4g of p-hydroxyacetophenone, 25 15g of ceteareth-6, 30g of cetearyl alcohol, 30g of liquid paraffin, 10g of petrolatum, 0.5g of butylated hydroxytoluene, 10g of polydimethylsiloxane, 10g of ethylhexyl palmitate, 1g of bisabolol, 5g of tocopherol acetate, 5g of 1, 2-hexanediol, 1g of a fragrance, and 680.3g of purified water.

The preparation method comprises the following steps: the raw materials are prepared according to the formula. Adding purified water, EDTA disodium, allantoin, p-hydroxyacetophenone and cetostearyl polyether-25 into a water phase pot, and starting to heat to 80-85 ℃. The glycerol, the butanediol and the xanthan gum are stirred and dispersed uniformly in advance by a container and then are added into a water phase pot. The water phase pot is stirred for 30 minutes under heat preservation. Adding ceteareth-6, cetearyl alcohol, liquid paraffin, petrolatum, butylated hydroxytoluene, polydimethylsiloxane and ethylhexyl palmitate into an oil phase pot, starting to heat to 80-85 ℃, and stirring until the oil phase materials are completely dissolved. Firstly, adding the water phase material into an emulsifying pot, then adding the oil phase material into the emulsifying pot, starting homogenizing for 5 minutes, stirring for 10 minutes, and then, starting to cool. Cooling to 45 deg.C, adding bisabolol, tocopheryl acetate and essence, and stirring. The temperature was reduced to 35 ℃ and the solution containing composition III prepared in example 10 and 1, 2-hexanediol were added and stirred well. Filtering with 200 mesh filter screen.

In a word, the invention provides a composition which takes nicotinamide adenine dinucleotide as a main component and is compatible with one or two of palmitoyl tripeptide-1 and sodium ascorbyl phosphate. The composition of the invention has outstanding anti-wrinkle, whitening and moisturizing effects. Therefore, the invention also provides the application of the composition in preparing the composition for resisting skin aging.

Then, the temperature is decreased. Cooling to 65 deg.C, adding triethanolamine, and stirring. Cooling to 45 deg.C, adding bisabolol, tocopheryl acetate and essence, and stirring. The temperature was reduced to 35 ℃ and 100g of the solution containing composition II prepared in example 8 and 1, 2-hexanediol were added and stirred well. Filtering with 200 mesh filter screen.

Example 10Combination of two or moreSubstance III and solutions containing the same

The composition of composition III (500 g) described in this example is:

NAD +300g and sodium ascorbyl phosphate 100g

NAD +300g, sodium ascorbyl phosphate 100g and purified water 600g were weighed. Mixing the three solutions, and stirring to obtain target solution; storing at-15 to-20 ℃ to prevent the growth of microorganisms.

Example 11Anti-aging emulsion

The anti-aging emulsion (1000 g) described in this example comprises the following raw materials: 100g of the solution containing the composition III prepared in example 10, 50g of glycerin, 30g of butanediol, 2g of xanthan gum, 0.2g of disodium EDTA, 1g of allantoin, 4g of p-hydroxyacetophenone, 25 15g of ceteareth-25, 6 15g of ceteareth-6, 30g of cetearyl alcohol, 30g of liquid paraffin, 10g of petrolatum, 0.5g of butylated hydroxytoluene, 10g of polydimethylsiloxane, 10g of ethylhexyl palmitate, 1g of bisabolol, 5g of tocopherol acetate, 5g of 1, 2-hexanediol, 1g of essence, and 680.3g of purified water.

The preparation method comprises the following steps: the raw materials are prepared according to the formula. Adding purified water, EDTA disodium, allantoin, p-hydroxyacetophenone and cetostearyl polyether-25 into a water phase pot, and starting to heat to 80-85 ℃. The glycerol, the butanediol and the xanthan gum are stirred and dispersed uniformly in advance by a container and then are added into a water phase pot. The water phase pot is stirred for 30 minutes under heat preservation. Adding ceteareth-6, cetearyl alcohol, liquid paraffin, petrolatum, butylated hydroxytoluene, polydimethylsiloxane and ethylhexyl palmitate into an oil phase pot, starting to heat to 80-85 ℃, and stirring until the oil phase materials are completely dissolved. Firstly, adding the water phase material into an emulsifying pot, then adding the oil phase material into the emulsifying pot, starting homogenizing for 5 minutes, stirring for 10 minutes, and then, beginning to cool. Cooling to 45 deg.C, adding bisabolol, tocopheryl acetate and essence, and stirring. The temperature was reduced to 35 ℃ and the solution containing composition III prepared in example 10 and 1, 2-hexanediol were added and stirred well. Filtering with 200 mesh filter screen.

In conclusion, the invention provides a composition which takes nicotinamide adenine dinucleotide as a main component and is compatible with one or two of palmitoyl tripeptide-1 and sodium ascorbyl phosphate. The composition of the invention has outstanding anti-wrinkle, whitening and moisturizing effects. Therefore, the invention also provides the application of the composition in preparing the composition for resisting skin aging.

Claims

1. A composition comprises 3 parts by weight of nicotinamide adenine dinucleotide, 0.75 parts by weight of palmitoyl tripeptide-1 and 1.25 parts by weight of sodium ascorbyl phosphate.

2. The preparation method of the composition as claimed in claim 1, which comprises mixing the components uniformly according to the mass ratio.

3. An anti-skin aging composition comprising the composition of claim 1, a cosmetic additive and a skin external acceptable adjuvant; wherein, based on the mass of the composition for resisting skin aging, the composition in claim 1 has a mass percentage of 2.75% -11%, and the cosmetic additive has a mass percentage of 1% -10%; the cosmetic additive is selected from one or more of allantoin, bisabolol, panthenol, aloe extract and dipotassium glycyrrhizinate.

4. The anti-skin aging composition according to claim 3, wherein the composition of claim 1 is present in an amount of 4.25% to 9% by mass based on the mass of the anti-skin aging composition.

5. The anti-skin aging composition according to claim 4, wherein the composition of claim 1 is present in an amount of 4.75% to 8% by mass based on the mass of the anti-skin aging composition.

6. The anti-skin aging composition according to any one of claims 3 to 5, wherein the anti-skin aging composition is one or more of a water agent, an emulsion, a cream, an ointment, an oil agent and a mask.

7. A method for preparing the composition for resisting skin aging according to any one of claims 3 to 5, which comprises preparing the composition of claim 1, the cosmetic additive and the externally acceptable auxiliary materials for skin into a required dosage form according to a conventional method in the art.