CN110292961B - Sealing structure of pipette tip - Google Patents
Sealing structure of pipette tip Download PDFInfo
- Publication number
- CN110292961B CN110292961B CN201910587614.7A CN201910587614A CN110292961B CN 110292961 B CN110292961 B CN 110292961B CN 201910587614 A CN201910587614 A CN 201910587614A CN 110292961 B CN110292961 B CN 110292961B
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- sealing
- connecting flange
- pressing plate
- plate
- shell
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- 238000007789 sealing Methods 0.000 title claims abstract description 142
- 238000003825 pressing Methods 0.000 claims abstract description 35
- 239000004816 latex Substances 0.000 claims abstract description 12
- 229920000126 latex Polymers 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims description 22
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 16
- 238000011194 good manufacturing practice Methods 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 10
- 238000012546 transfer Methods 0.000 claims description 10
- 239000007789 gas Substances 0.000 claims description 8
- 239000011368 organic material Substances 0.000 claims description 2
- 238000002955 isolation Methods 0.000 abstract description 5
- 238000009169 immunotherapy Methods 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000005086 pumping Methods 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004880 explosion Methods 0.000 description 2
- 239000003566 sealing material Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Coating Apparatus (AREA)
- Devices For Use In Laboratory Experiments (AREA)
Abstract
The invention relates to the technical field of immunotherapy pipette tip sealing, in particular to a pipette tip sealing structure which comprises a sealing shell, wherein the sealing shell comprises an upper sealing shell and a lower sealing shell, the upper sealing shell and the lower sealing shell are arranged, a fixed flange pressing plate structure is fixedly arranged at the central position of the bottom of the lower sealing shell and comprises a fixed flange pressing plate, a sealing ring and a pressing plate, the bottom of the fixed flange pressing plate structure is connected with a sealing sleeve, the bottom of the sealing sleeve is provided with a double-shaft latex sleeve, a double-shaft pipette tip is sleeved inside the double-shaft latex sleeve, and the bottom of the fixed flange pressing plate is fixedly provided with a sealing groove. Effectively isolating the precise electronic components and sterilizing gas in the isolation period.
Description
Technical Field
The invention relates to the technical field of immunotherapy pipette tip sealing, in particular to a pipette tip sealing structure.
Background
Immunotherapy and regenerative medicine enter a large-scale application stage, the traditional manual laboratory production mode is far from meeting the requirements of large-scale industrial production, the cost is high, the efficiency is low, the stability is low, the production environment is limited, and the data of the production process cannot be traced, so that the application bottleneck of the traditional production mode is formed. Under this background, full-automatic intelligent cell culture and check out test set come into production in due charge, compare in traditional mode, it is high-efficient, and is stable, low cost, arrange nimble and the controllable advantage of full flow data, but in the full-automatic cell culture flow, the automation that needs carry out all kinds of biological reagents in a large number moves the liquid, the pump liquid operation, the barrier propterty is poor, thereby can not guarantee to move liquid quality and efficiency, further make its inside liquid receive the pollution, for realizing GMP compliance, require the operational environment must be A level clean room, move liquid/pump liquid module must be a totally enclosed independent unit simultaneously.
In summary, the present invention solves the existing problems by designing a sealing structure for a pipette tip.
Disclosure of Invention
The invention aims to provide a sealing structure of a pipette tip, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a sealing structure of a pipette tip comprises a sealing shell, wherein the sealing shell comprises an upper sealing shell and a lower sealing shell, the upper sealing shell and the lower sealing shell are fixedly provided with a fixed flange pressing plate structure at the bottom center position of the lower sealing shell, the fixed flange pressing plate structure comprises a fixed flange pressing plate, a sealing ring and a pressing plate, the bottom of the fixed flange pressing plate structure is connected with a sealing sleeve, the bottom of the sealing sleeve is provided with a double-shaft latex sleeve, the double-shaft pipette tip is sleeved inside the double-shaft latex sleeve, the bottom of the fixed flange pressing plate is fixedly provided with a sealing groove, the four corners inside the sealing groove are fixedly provided with a first threaded hole on the fixed flange pressing plate, the sealing groove is fixedly provided with a sealing ring, the four corners of the sealing ring are fixedly provided with second threaded holes, and the pressing plate is arranged above the sealing ring and inside the sealing groove, the four corners of the pressure plate are fixedly provided with third threaded holes, the sealing sleeve is arranged below the sealing ring in the sealing groove and sequentially passes through the third threaded holes, the second threaded holes and the first threaded holes by bolts to connect the pressure plate, the sealing ring and the fixed flange pressure plate, a first upper connecting flange plate and a second upper connecting flange plate are symmetrically arranged at the bottom of the lower sealing shell based on the fixed flange pressure plate structure as an axis, the first upper connecting flange is connected with a first lower connecting flange through bolts, a first hollow pillar is sleeved inside the first lower connecting flange, the bottom of the first hollow strut is sleeved inside the first flange plate, the second upper connecting flange plate is connected with a second lower connecting flange plate through bolts, and a second hollow strut is sleeved in the second lower connecting flange plate, and the bottom of the second hollow strut is sleeved in the second flange plate.
Preferably, the upper sealing shell and the lower sealing shell are made of materials which can endure 30% hydrogen peroxide gas for a long time.
Preferably, the joints of the upper sealing shell and the lower sealing shell are both provided with bottom plates and connected through screws, and gaps at the joints of the upper sealing shell and the lower sealing shell are filled with trial materials inside the isolator.
Preferably, the pressure plate, the first hollow strut and the second hollow strut are made of a material capable of enduring 30% concentration hydrogen peroxide gas for a long time.
Preferably, a liquid pipeline channel is fixedly arranged inside the first hollow support column, an electric cable is fixedly arranged inside the second hollow support column, and the end parts of the first hollow support column and the second hollow support column respectively penetrate through the first upper connecting flange plate and the second upper connecting flange plate and extend to the inside of the sealed shell.
Preferably, the joint of the first upper connecting flange and the first lower connecting flange and the joint of the second upper connecting flange and the second lower connecting flange are both fixedly provided with sealing tape gaskets.
Preferably, the bottom of the pressure plate is fixedly provided with a groove and is arranged corresponding to the sealing groove and the sealing ring.
Preferably, the sealing sleeve is a high-elasticity organic material film sealing sleeve and is made of a material which can endure 30% hydrogen peroxide gas for a long time and meets GMP (good manufacturing practice) compliance.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, by designing the sealing structure of the pipette tip, the sealing requirement of the pipetting/liquid pumping module in the full-automatic cell culture process is realized on the premise of GMP (good manufacturing practice) compliance, the isolation of the inner cavity of the pipette tip unit from the inner region of the isolator is realized, and the precise electronic components and the sterilization and disinfection gas in the isolation period are effectively isolated.
2. In the invention, the sealing structure parts are made of materials which can endure 30% of hydrogen peroxide vapor for a long time, so that the reliability of equipment in the disinfection process is ensured; the ductility and the telescopic performance of the latex are utilized to ensure the motion sealing of the gun head; the modular quick-plug design is convenient for the disassembly and replacement of the front sealing sleeve; the sealing material meets GMP (good manufacturing practice) compliance, and can ensure the realization of automatic liquid transfer and liquid pumping operation of the GMP compliance.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic diagram of a partial explosion of the present invention;
FIG. 3 is a schematic structural view of the present invention;
FIG. 4 is an enlarged structural view of the fixed flange pressing plate structure of the present invention;
fig. 5 is a schematic diagram of an explosion structure of the fixed flange pressure plate of the present invention.
In the figure: 1-a sealing shell, 2-an upper sealing shell, 3-a lower sealing shell, 4-a fixed flange pressure plate structure, 5-a fixed flange pressure plate, 6-a sealing ring, 7-a pressure plate, 8-a sealing sleeve, 9-a double-shaft emulsion sleeve, 10-a double-shaft pipette tip, 11-a second flange plate, 12-a sealing groove, 13-a first threaded hole, 14-a second threaded hole, 15-a third threaded hole, 16-a first upper connecting flange plate, 17-a first lower connecting flange plate, 18-a first hollow support column, 19-a first flange plate, 20-a second upper connecting flange plate, 21-a second lower connecting flange plate and 22-a second hollow support column.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a technical solution:
a sealing structure of a pipette tip comprises a sealing shell 1, wherein the sealing shell 1 comprises an upper sealing shell 2 and a lower sealing shell 3, the upper sealing shell 2 and the lower sealing shell 3 are respectively fixedly provided with a fixed flange pressing plate structure 4 at the bottom center position of the lower sealing shell 3, the fixed flange pressing plate structure 4 comprises a fixed flange pressing plate 5, a sealing ring 6 and a pressing plate 7, the bottom of the fixed flange pressing plate structure 4 is connected with a sealing sleeve 8, the bottom of the sealing sleeve 8 is provided with a double-shaft latex sleeve 9, a double-shaft pipette tip 10 is sleeved inside the double-shaft latex sleeve 9, the bottom of the fixed flange pressing plate 5 is fixedly provided with a sealing groove 12, four corners inside the sealing groove 12 are fixedly provided with first threaded holes 13 on the fixed flange pressing plate 5, the sealing ring 6 is fixedly arranged inside the sealing groove 12, and the four corners of the sealing ring 6 are all fixedly provided with second threaded holes 14, a press plate 7 is arranged above the sealing ring 6 and inside the sealing groove 12, third threaded holes 15 are fixedly arranged at the four corners of the press plate 7, a sealing sleeve 8 is arranged below the sealing ring 6 inside the sealing groove 12 and sequentially passes through the third threaded holes 15, the second threaded holes 14 and the first threaded holes 13 by bolts to connect the press plate 7, the sealing ring 6 and the fixed flange press plate 5, a first upper connecting flange 16 and a second upper connecting flange 20 are symmetrically arranged at the bottom of the lower sealing shell 3 based on the fixed flange press plate structure 4 as an axis, the first upper connecting flange 16 is connected with a first lower connecting flange 17 by bolts, a first hollow strut 18 is sleeved inside the first lower connecting flange 17, the bottom of the first hollow strut 18 is sleeved inside the first flange 19, and the second upper connecting flange 20 is connected with a second lower connecting flange 21 by bolts, the second lower connecting flange 21 is sleeved with a second hollow pillar 22, and the bottom of the second hollow pillar 22 is sleeved inside the second flange 11.
The working process of the invention is as follows: when the device is used, the liquid transfer gun head sealing structure is fixed on a liquid operation platform by a first flange 19 and a second flange 11, the liquid transfer device 3 is assembled in the sealing shell 1 formed by the upper sealing shell 2 and the lower sealing shell 3, a liquid pipeline channel is fixedly arranged in the first hollow strut 18, an electric cable is fixedly arranged in the second hollow strut 22 to install a liquid transfer circuit and management, after the installation is completed, the sealing sleeve 8 is arranged below the sealing ring 14 in the sealing groove 12 and sequentially passes through the bolt, the third threaded hole 15, the second threaded hole 14 and the first threaded hole 13 to connect the pressing plate 7, the sealing ring 6 and the fixed flange pressing plate 5 together, after the connection is completed, the double-shaft liquid transfer gun head 10 is sleeved by the double-shaft latex sleeve 9, and the double-shaft liquid transfer gun head 10 is extended to the outside of the double-shaft latex sleeve 9 to form a sealing structure, in the process, by designing a liquid transfer gun head sealing structure, on the premise of GMP (good manufacturing practice) compliance, the sealing requirement of a liquid transfer/pumping module in a full-automatic cell culture process is met, the isolation of an inner cavity of a gun head unit from an area inside an isolator is realized, a precise electronic component and sterilization and disinfection gas in an isolation period are effectively isolated, and a material capable of enduring 30% hydrogen peroxide vapor for a long time is adopted by sealing structure parts, so that the reliability of equipment in the disinfection process is ensured; the ductility and the telescopic performance of the latex are utilized to ensure the motion sealing of the gun head; the modular quick-plug design is convenient for the disassembly and replacement of the front sealing sleeve; the sealing material meets GMP (good manufacturing practice) compliance, and can ensure the realization of automatic liquid transfer and liquid pumping operation of the GMP compliance.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a pipette tip seal structure, includes seal housing (1), its characterized in that: the sealing shell (1) comprises an upper sealing shell (2) and a lower sealing shell (3), the upper sealing shell (2) and the lower sealing shell (3) are fixedly arranged at the center of the bottom of the lower sealing shell (3), a fixing flange pressing plate structure (4) is fixedly arranged on the center of the bottom of the lower sealing shell (3), the fixing flange pressing plate structure (4) comprises a fixing flange pressing plate (5), a sealing ring (6) and a pressing plate (7), the bottom of the fixing flange pressing plate structure (4) is connected with a sealing sleeve (8), a double-shaft latex sleeve (9) is arranged at the bottom of the sealing sleeve (8), a double-shaft liquid-transfer gun head (10) is sleeved in the double-shaft latex sleeve (9), a sealing groove (12) is fixedly formed in the bottom of the fixing flange pressing plate (5), a first threaded hole (13) is fixedly formed in the four corners of the inside of the sealing groove (12) and positioned on the fixing flange pressing plate (5), a sealing ring (6) is fixedly arranged inside the sealing groove (12), second threaded holes (14) are fixedly formed in the four corners of the sealing ring (6), a pressing plate (7) is arranged above the sealing ring (6) and inside the sealing groove (12), third threaded holes (15) are fixedly formed in the four corners of the pressing plate (7), the sealing sleeve (8) is arranged below the sealing groove (12) and inside the sealing ring (6) and sequentially passes through the third threaded holes (15), the second threaded holes (14) and the first threaded holes (13) through bolts to connect the pressing plate (7), the sealing ring (6) and the fixed flange pressing plate (5), a first upper connecting flange plate structure (16) and a second upper connecting flange plate structure (20) are symmetrically arranged at the bottom of the lower sealing shell (3) for an axis based on the fixed flange pressing plate (4), the connecting flange plate is characterized in that a first lower connecting flange plate (17) is connected to the first upper connecting flange plate (16) through bolts, a first hollow support column (18) is sleeved inside the first lower connecting flange plate (17), the bottom of the first hollow support column (18) is sleeved inside the first flange plate (19), a second lower connecting flange plate (21) is connected to the second upper connecting flange plate (20) through bolts, a second hollow support column (22) is sleeved inside the second lower connecting flange plate (21), and the bottom of the second hollow support column (22) is sleeved inside the second flange plate (11).
2. A pipette tip sealing structure according to claim 1, characterized in that: the upper sealing shell (2) and the lower sealing shell (3) are made of materials which can resist 30% concentration hydrogen peroxide gas for a long time.
3. A pipette tip sealing structure according to claim 1, characterized in that: the joint of the upper sealing shell (2) and the lower sealing shell (3) is provided with a bottom plate and is connected through screws, and gaps of the joint of the upper sealing shell (2) and the lower sealing shell (3) are filled with trial materials inside the isolator.
4. A pipette tip sealing structure according to claim 1, characterized in that: the pressure plate (7), the first hollow support column (18) and the second hollow support column (22) are made of materials which can resist 30% concentration hydrogen peroxide gas for a long time.
5. A pipette tip sealing structure according to claim 1, characterized in that: the inner part of the first hollow support column (18) is fixedly provided with a liquid pipeline channel, the inner part of the second hollow support column (22) is fixedly provided with an electric cable, and the end parts of the first hollow support column (18) and the second hollow support column (22) respectively penetrate through the first upper connecting flange plate (16) and the second upper connecting flange plate (20) and extend to the inner part of the sealing shell (1).
6. A pipette tip sealing structure according to claim 1, characterized in that: and sealing belt gaskets are fixedly arranged at the joint of the first upper connecting flange plate (16) and the first lower connecting flange plate (17) and the joint of the second upper connecting flange plate (20) and the second lower connecting flange plate (21).
7. A pipette tip sealing structure according to claim 1, characterized in that: the bottom of the pressure plate (7) is fixedly provided with a groove and is arranged corresponding to the sealing groove (12) and the sealing ring (6).
8. A pipette tip sealing structure according to claim 1, characterized in that: the sealing sleeve (8) is a high-elasticity organic material film sealing sleeve and is made of a material which can endure 30% hydrogen peroxide gas for a long time and meets GMP (good manufacturing practice) compliance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910587614.7A CN110292961B (en) | 2019-07-02 | 2019-07-02 | Sealing structure of pipette tip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910587614.7A CN110292961B (en) | 2019-07-02 | 2019-07-02 | Sealing structure of pipette tip |
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| Publication Number | Publication Date |
|---|---|
| CN110292961A CN110292961A (en) | 2019-10-01 |
| CN110292961B true CN110292961B (en) | 2021-07-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910587614.7A Active CN110292961B (en) | 2019-07-02 | 2019-07-02 | Sealing structure of pipette tip |
Country Status (1)
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| CN (1) | CN110292961B (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130039125A (en) * | 2011-10-11 | 2013-04-19 | 삼성테크윈 주식회사 | Nozzle assembly for air displacement pipettor |
| CN104968269A (en) * | 2012-12-05 | 2015-10-07 | 赛拉诺斯股份有限公司 | Systems, devices and methods for bodily fluid sample collection, transport and processing |
| CN105510109A (en) * | 2015-11-30 | 2016-04-20 | 武汉璟泓万方堂医药科技股份有限公司 | Blood sample sampling pretreatment device and method |
| CN207667672U (en) * | 2017-12-22 | 2018-07-31 | 吴江华衍水务有限公司 | A kind of liquid transfer gun head |
| CN208542220U (en) * | 2018-05-14 | 2019-02-26 | 黄河科技学院 | A medical test cup |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US6528026B2 (en) * | 1998-08-13 | 2003-03-04 | Symyx Technologies, Inc. | Multi-temperature modular reactor and method of using same |
| US7250139B2 (en) * | 2003-03-19 | 2007-07-31 | Northwestern University | Nanotipped device and method |
| EP3865875A1 (en) * | 2011-09-25 | 2021-08-18 | Labrador Diagnostics LLC | Systems and methods for multi-analysis |
| CA3061355C (en) * | 2017-06-08 | 2024-02-13 | Integra Biosciences Ag | Sample and reagent reservoir kits and liners with anti-vacuum feature |
| CN108080048B (en) * | 2017-12-28 | 2024-01-02 | 北京理工大学 | Double-end liquid-transfering gun of interval adjustable |
-
2019
- 2019-07-02 CN CN201910587614.7A patent/CN110292961B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130039125A (en) * | 2011-10-11 | 2013-04-19 | 삼성테크윈 주식회사 | Nozzle assembly for air displacement pipettor |
| CN104968269A (en) * | 2012-12-05 | 2015-10-07 | 赛拉诺斯股份有限公司 | Systems, devices and methods for bodily fluid sample collection, transport and processing |
| CN105510109A (en) * | 2015-11-30 | 2016-04-20 | 武汉璟泓万方堂医药科技股份有限公司 | Blood sample sampling pretreatment device and method |
| CN207667672U (en) * | 2017-12-22 | 2018-07-31 | 吴江华衍水务有限公司 | A kind of liquid transfer gun head |
| CN208542220U (en) * | 2018-05-14 | 2019-02-26 | 黄河科技学院 | A medical test cup |
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| CN110292961A (en) | 2019-10-01 |
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