CN110279034A - Production process of oregano oil product for livestock and self-emulsifying preparation product - Google Patents

Abstract

The invention discloses a manufacturing process of a product and a self-emulsifying preparation product (self-emulsifying drug delivery system medicinal fruit juice) of oregano oil for livestock, and discloses the technical field of mixed feed, in particular to the manufacturing process of the product and the self-emulsifying preparation product of the oregano oil for livestock, which are developed by developing a self-emulsifying drug delivery system or a drug delivery system of the oregano oil and the stability of the drug delivery system product of the oregano oil. The product has also been developed to be effective against bacteria causing certain diseases in chicken and to promote antioxidant activity in the chicken intestinal system. The product is also designed in a form convenient as a food additive in chicken, the ingredients of which are beneficial to the animal and economically cost effective.

Description

Production process of oregano oil product for livestock and self-emulsifying preparation product

technical field

The invention relates to the technical field of mixed feed, and the specific field is the production process of oregano oil products for livestock and self-emulsifying preparation products (medicinal fruit juice of self-emulsifying drug delivery system).

Background technique

Currently, world livestock production policy focuses on reducing the use of antibiotics and chemicals, making them safer for animals and consumers. Through the research and application of medicinal plant extracts, alternatives to antibiotics are developed to improve yield and egg quality. Natural plants such as sweet basil, holy basil, finger root, pepper, etc. play an important role in the prevention and treatment of diseases in chickens. Extracts or oils of these natural plants have been used as food ingredients. According to reports, sweet basil has a positive inhibitory effect on chicken dysentery, is a food additive that can replace antibiotics, and can be used to prevent chicken dysentery. In addition, many research reports have confirmed that oregano extract has antibacterial effects, antioxidant activity, and helps to increase the food intake of poultry (chicken, duck, quail).

Studies have also shown that using oregano oil extract has the same effect as using chemical or antibiotic substances, but with the benefit that oregano oil has minimal side effects in animals and is not carcinogenic. However, the solubility of these extracts or oils is low, and the fat dosage in chicken is low, 0.4-1 μl/ml. Therefore, when these fats are dissolved or mixed in poultry drinking water or liquid food, there are problems of insolubility and separation of fats from the system, so that chickens cannot ingest oil supplements with effective amounts of bioactive compounds.

For this reason, the production process of oregano oil for livestock and self-emulsifying formula products (medicinal juice for self-emulsifying drug delivery system) were developed to solve such problems.

Drug delivery systems are the preparation of drugs or substances in various forms that facilitate modification such as controlled release of the drug or substance in a desired amount and duration, delivery of the drug or substance to the target area or organ to reduce side effects or to maximize the treatment effect. In veterinary medicine, the main goal of delivery systems is to focus on controlled drug release in order to reduce the stress of forced or repeated drug or substance introduction into animals, thereby reducing the cost of labor and time required for veterinary practice. Examples of drug groups to which controlled drug or veterinary drug release techniques have been applied are antimicrobial drugs, helminth drugs, vitamins, minerals, growth stimulants, hormones, etc. Examples of delivery systems with various forms of controlled drug release in veterinary applications are e.g. oral delivery systems, intravaginal delivery systems, subcutaneous implantable delivery systems, injectable delivery systems, topical delivery systems and transdermal delivery systems, Ocular delivery systems, intramammary delivery systems and nanomedicine.

Some active ingredients cannot be processed into salt form, or may form precipitates in the gastrointestinal tract, and have complex development or production formulations, which in the present invention are developed to support the delivery of active ingredients and water-insoluble drugs,

The present invention relates to a novel animal feed composition, for example containing a benzoic acid derivative or a metabolite of the acid as active ingredient, and a mixture of at least two active compounds selected from the group consisting of thymol, eugenol and piperine. The inventors have found that, in addition to the known function of benzoic acid, this compound can also act as a growth promoter when combined with a mixture of at least two active compounds selected from the above group.

In particular, the inventors have shown that mixtures of these compounds present in various parts of the plant have a synergistic effect on each other and, when used together with appropriate amounts of benzoic acid, improve the digestion of poultry food, i.e. increase the animal's daily Food conversion or weight gain.

Contents of the invention

The purpose of the present invention is to provide a production process of oregano oil product for livestock and a self-emulsifying preparation product (self-emulsifying drug delivery system medicinal fruit juice), so as to solve the problems raised in the above-mentioned background technology.

In order to achieve the above object, the present invention provides the following technical solutions: the production process of oregano oil products for livestock and self-emulsifying preparation products (self-emulsifying drug delivery system medicinal fruit juice),

1) Composition of raw materials

Oregano oil accounts for 80% to 90% by weight

Span®80 accounts for 5% to 15% by weight

Tween®80 accounts for 5% to 10% by weight.

2) Manufacturing process of oregano oil for livestock

Mix oregano oil with Span®80 and Tween®80 uniformly, and the method is not limited to rotation, stirring, centrifugation, swirl or automatic machinery to obtain oregano oil product.

Preferably, the oregano oil product is filled in the soft capsules by molding through a soft capsule filling process, and in order to make the capsule firm and stable, use a humidity regulator or make the soft capsule product in a dehumidification chamber.

Preferably, the ratio of Span 80 to Tween 80 is 1:1-1:2.5.

Preferably, oregano oil is mixed with Span®80 and Tween®80 at a temperature of 25-30°C, at a speed of 400-500 rpm, and continues to rotate for 8-24 hours.

Preferably, the material used to prepare the soft capsule is gelatin or a material containing gelatin.

Preferably, wherein the soft capsule can have various shapes: granular, spherical or oval.

Preferably, wherein the capsule product is packed in a plastic bottle by a packaging machine to reduce moisture.

Preferably, the oregano oil product manufacturing process for domestic animals and the self-emulsifying preparation product (medicinal fruit juice for self-emulsifying drug delivery system) contain smaller oil droplets and have good dispersibility, so it can solve the problem caused by indigestion of oil. flatulence problem.

Preferably, the oregano oil product manufacturing process and self-emulsifying preparation product (self-emulsifying drug delivery system medicinal fruit juice) for domestic animals can be made into soft and ready-to-eat health supplement food formulated with oregano oil capsules.

The beneficial effect of the present invention is: the production process of oregano oil product for livestock and self-emulsifying preparation product (medicinal fruit juice of self-emulsifying drug delivery system), the product of oregano oil (self-emulsifying drug delivery system medicinal fruit juice) for livestock , the manufacturing process and self-emulsifying formulation were developed as a drug or substance delivery system in the form of a self-occurring nanoemulsion (Self-Emulsifying Drug Delivery System from Oregano Oil or SEDDS), which is effective against bacteria that cause certain diseases in chickens And promote the antioxidant activity in chicken intestinal system. The self-emulsifying drug delivery system of oregano oil is designed for convenient use as a food additive in chicken meat, and with stability for storage and extended shelf-life, the invention's animal-friendly ingredient development is also economically cost-effective. The present invention is a unique targeted delivery system for improving gut health in poultry, aided by the use of innovative mineral chelators, with the aim of delivering substances to target organs, reducing side effects and positively affecting function. Positively affects the gut of poultry by effectively creating an optimal environment for digestion and absorption. Effectively increases nutrient uptake, which benefits animals, increases food value for consumers, and increases production efficiency.

Description of drawings

Figure 1 shows the process of developing and producing oregano oil product manufacturing process for livestock and self-emulsifying preparation product (self-emulsifying drug delivery system medicinal juice);

Various formulations of the self-emulsifying product of Fig. 2 oregano oil;

Figure 3 Images from the microscope showing the appearance of self-emulsified products of various formulations of oregano oil after 24 hours of 500-fold dilution with water;

Figure 4 reads the chromatogram values of the standard compounds p-cymene, terpinene, thymol, carvacrol and caryophyllene by GC-MS.

Detailed ways

The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Example:

The present invention is a unique targeted delivery system for improving the intestinal health of poultry, supplemented by the use of innovative mineral chelators, with the aim of directing substances into target organs, reducing side effects and improving digestion and absorption by effectively creating ( gut health) has a positive effect on gut function in poultry. Helps increase nutrient uptake, which benefits animals, increases food value for consumers, and increases production efficiency.

Increase egg production efficiency by improving the nutrition and quality of laying hens. By selecting high-quality raw materials to extract herbal medicines such as oregano oil, good egg production can be obtained, and eggs that meet quality standards (AA grade) can be produced to meet the market. Eggs also have market properties such as strong shells, visibly separable whites, nutritional value additive eggs (functional eggs), etc.

Production process of oregano oil products for livestock and self-emulsifying preparation products (self-emulsifying drug delivery system medicinal juice)

1) Composition of raw materials

Oregano oil accounts for 80% to 90% by weight

Span®80 accounts for 5% to 15% by weight

Tween®80 accounts for 5% to 10% by weight.

2) Manufacturing process of oregano oil for livestock

Mix oregano oil with Span®80 and Tween®80 evenly to get oregano oil product.

Molded by soft capsule filling process, the oregano oil product is filled into soft capsules, in order to make the capsule firm and stable, the soft capsule product is manufactured using a humidity regulator or in a dehumidification chamber.

The ratio of Span 80 and Tween 80 is 1:1～1:2.5.

Mix oregano oil with Span®80 and Tween®80 at a temperature of 25-30°C, at a speed of 400-500 rpm, and continue to rotate for 8-24 hours.

Wherein the material used to prepare the soft capsule is gelatin or a material containing gelatin.

Wherein said soft capsule can have multiple shapes: granular, spherical or oval.

Wherein said capsule product is packed in plastic bottle by packing machine, reduces moisture, and storage time prolongs.

After the above steps, the oregano oil product manufacturing process for livestock and the self-emulsifying preparation product (medicinal juice for self-emulsifying drug delivery system) contain smaller oil droplets and have good dispersibility, so it can solve the problem of indigestion of oil Caused gas problems. Among them, the manufacturing process of oregano oil products for livestock and self-emulsifying preparation products (self-emulsifying drug delivery system medicinal juice) can be made into soft and ready-to-eat health supplements formulated with oregano oil capsules.

Experiment 1 Development of a drug delivery system in the form of self-emulsifying oregano oil

By discovering the balance between hydrophilic and hydrophobic groups, also known as the NHLB (Hydrophile-Lipophile Balance, HLB) of basil oil and oregano oil, and in sorbitan esters (Span®) and polysorbate Esters (Tween®) to choose a suitable surfactant, store the formulated samples in glass jars with lids at room temperature to study stability (no separation) for at least 1 week, after completion, test the formulated samples for The particle size and charge of the surface particles are varied by adjusting the ratio until the desired characteristics are obtained.

Experiment 2 Experimental Research on Emulsion Formula Efficiency

360 chickens of the Lohmann Brown variety were used for the experiment, divided into 3 groups, 15 chickens in each group, and repeated 8 times. Laying hens were housed in closed-system houses with adequate food and water ad libitum access throughout the experiments. Experimental data collection was divided into 4 periods, each period consisted of 14 days, to study the effectiveness of the emulsion formulation.

Experiment 3 evaluates the properties of the developed self-emulsifying formulation

Particle size and charge on particle surfaces of self-emulsified oils from various dilutions of sweet basil oil and oregano oil were analyzed. According to the analysis results, the self-emulsifying formulations of sweet basil oil and oregano oil were selected, and the surfactants lecithin and Tween 80 in different ratios at a concentration of 20% were selected to evaluate the physical stability of the emulsion. Based on Figure 3, it was found that the emulsion using oregano oil had higher stability.

The results obtained from the physical stability of the emulsions formed by various formulations of oregano oil before and after dilution with water, including the particle size and charge on the particle surface, indicated that the suitable surfactant for the preparation of self-emulsifying formulations of sweet basil oil was Oregano oil and lecithin are lecithin and Tween 80 in a ratio of 3L/1T, and the optimal concentration of a pair of surfactants used for preparation is 80% (w/w).

Experiment 4 Analysis of important compounds present in raw oregano oil and self-emulsifying formula

Determination of important compounds in oregano oil and self-emulsifying preparations of oregano oil using GC-MS technique. In general, in oregano oil, the important compounds found are divided into large groups, namely, hydrocarbons (3-thujene, pinene, terpinene, p-cymene, terpinene, caryophyllene), alcohols (aromatic camphor, borneol, terpinen-4-ol), phenols (carvacrol, thymol) and esters (linalyl acetate) (Moschos G. Polissiou, 2000). The amount of each important compound in oregano oil depends on the season and the cultivated source. Therefore, the amount of 5 important types of compounds in oregano oil was analyzed, namely: p-cymene, terpinene, thymol, carvacrol, caryophyllene. According to the compound standards of the five compounds analyzed using GC-MS technique, the retention times were found to be 8.145, 8.951, 13.267, 13.408 and 15.181 min, respectively, as shown in the GC-MS chromatogram (Fig. 4). It was found that among the five compounds, oregano oil contained the highest amount of carvacrol based on the standard calibration curve, with an R2 (correlation coefficient) of 0.9950 and above.

Experiment 5 is to study the effect of oregano oil self-emulsifying formula on the development of laying hens, improve the value of eggs, meet the agricultural production standards, and increase the consumption value of consumers

A completely randomized experimental design was performed on 360 rear chickens of breed Lohmann Brown, which were divided into 3 groups, each consisting of 15 chickens, with 8 repetitions. Laying hens were housed in closed-system houses with adequate food and water ad libitum access throughout the experiments. During the experiment, data were recorded to assess efficiency, laying capacity and quality of eggs obtained. The results of this experiment are shown in Table 1 and the egg quality results are shown in Table 2 for self-emulsifying formulations supplemented with oregano oil in hen feed.

Table 1 Effect of self-emulsifying formula of oregano oil supplement in laying hen diet on productivity (average value of each experiment with 360 chickens per group)

egg production performance control group Add oregano oil to control group Feed consumption (g/bird/day) 114.43 ± 2.57 115.33 ± 2.36 Feed conversion ratio per 1 kg of eggs produced 2.03±0.06 2.00±0.06 Egg production (percentage) 91.42±1.72 92.65±0.57 Average egg weight (g/egg) 63.32±0.30 60.11 ± 2.38

Table 2 Effect of self-emulsifying formula of oregano oil in the feed of laying hens on egg quality* (average value of each experiment with 360 chickens in each group)

recorded data control group Add oregano oil to control group Broken eggs (percentage) 0.67 ± 0.06 0.43±0.02 Fecal stained eggs (percentage) 0.56±0.08 0.50±0.03 Shell thickness (percentage) 0.33±0.02 0.37 ± 0.02 hough unit 73.7±0.10 84.6±0.06

Results of the study at the experimental farm level found that supplementation of laying hens with a self-emulsifying formula of oregano oil did not result in differences in food intake and average egg weight, and that supplementation resulted in better egg production and Feed conversion ratio per 1 kg of eggs. Eggs obtained from chickens supplemented with a self-emulsifying formulation of oregano oil are classified as grade AA eggs according to United States Department of Agriculture (USDA) standards.

A herbal emulsion delivery system in the form of a self-emulsifying formulation of oregano oil for use as a food supplement for laying poultry was developed from various research and experimental results, the results of which are summarized below:

Based on a study of the types, amounts and ratios of surfactant pairs suitable for preparing oregano oil emulsion forms, it was found that using a pairing of lecithin (L) 15% and Tween® 80 (T) 5 in the formulation 3% or 3L/1T The ratio to the amount of oregano is 80% by weight. When mixed with water to form an emulsion, the resulting oil particles have a particle size of less than 200 nm and a particle surface charge of -56 mV. As a result of the analysis of important compounds in the raw oregano oil and the self-emulsifying formula developed, it was found that the oregano oil contains a large number of compounds, such as: p-cymene, terpinene, thymol, carvacrol, caryophyllene, The contents are 1.029%, 2.538%, 86.961% and 0.499%, respectively. In the self-emulsifying formula of oregano oil, the contents of p-cymene, terpinene, thymol, carvacrol and caryophyllene are 3.101%, 0.791%, 1.637%, 68.850% and 0.385%, respectively. The most important compound in oregano oil is carvacrol based on the amount. On the basis of evaluating the stability of self-emulsifying formulations of oregano oil, the formed emulsion form was found to be highly stable: no changes in chemical composition and physical stability were found after storage after an accelerated period of 1 month.

Supplementing laying hens with oregano oil in a self-emulsifying formula did not result in consumption and average egg weights different from controls, supplementation did have better egg production and feed conversion per 1 kg of eggs compared to controls .

Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes, modifications and substitutions can be made to these embodiments without departing from the principle and spirit of the present invention. and modifications, the scope of the invention is defined by the appended claims and their equivalents.

Claims (7)

1. domestic animal origanum oil product manufacture and self-emulsifiable preparation product (the medicinal fruit juice of self-emulsifying drug delivery system), It is characterized in that:

1) raw material form

Origanum oil accounts for 80%~90% by weight

80 account for 5%~15% by weight

80 account for 5%~10% by weight.

2) domestic animal origanum oil manufacturing process

By origanum oil with80 Hes80 are uniformly mixed, and obtain wild marjoram oil product.

2. domestic animal according to claim 1 origanum oil product manufacture and self-emulsifiable preparation product (self-emulsifying drug The medicinal fruit juice of transmission system), it is characterised in that: it is moulded by soft capsule fill process, wild marjoram oil product is filled into soft capsule In, in order to keep capsule firm and stablize, soft gel products are manufactured using humidistat or in moist chamber.

3. according to claim 1 with domestic animal described in any one of 2 origanum oil product manufacture and self-emulsifiable preparation product (the medicinal fruit juice of self-emulsifying drug delivery system), it is characterised in that: Span80 and Tween80 ratio is 1:1~1:2.5.

4. domestic animal according to any one of claim 1 to 3 origanum oil product manufacture and self-emulsifiable preparation product (the medicinal fruit juice of self-emulsifying drug delivery system), it is characterised in that: origanum oil with80 Hes80 mixing temperatures 25~30 DEG C, speed is 400~500 revs/min, continues to rotate for 8~24 hours.

5. domestic animal according to any one of claim 1 to 4 origanum oil product manufacture and self-emulsifiable preparation product (the medicinal fruit juice of self-emulsifying drug delivery system), it is characterised in that: the material for being wherein used to prepare soft capsule is gelatin or contains The material of gelatin.

6. domestic animal according to any one of claim 1 to 5 origanum oil product manufacture and self-emulsifiable preparation product (the medicinal fruit juice of self-emulsifying drug delivery system), it is characterised in that: wherein the soft capsule can have various shapes: particle Shape, spherical or ellipse.

7. domestic animal according to any one of claim 1 to 6 origanum oil product manufacture and self-emulsifiable preparation product (the medicinal fruit juice of self-emulsifying drug delivery system), it is characterised in that: wherein the capsule product is packaged in plastics by packing machine In bottle, moisture is reduced.