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CN110226387A - A kind of speed sprouts cistanche deserticola seed particle and its preparation process - Google Patents

A kind of speed sprouts cistanche deserticola seed particle and its preparation process Download PDF

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Publication number
CN110226387A
CN110226387A CN201910157373.2A CN201910157373A CN110226387A CN 110226387 A CN110226387 A CN 110226387A CN 201910157373 A CN201910157373 A CN 201910157373A CN 110226387 A CN110226387 A CN 110226387A
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cistanche
seed
seeds
present
preparation process
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王进
陈拓
章高森
冯起
刘蔚
颜霞
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Hexi University
Northwest Institute of Eco Environment and Resources of CAS
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Hexi University
Cold and Arid Regions Environmental and Engineering Research Institute of CAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06Coating or dressing seed

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  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

一种速萌肉苁蓉种子颗粒及其制备工艺。本发明公开了一种肉苁蓉种子颗粒、制备工艺及肉苁蓉的种植方法,所述肉苁蓉种子颗粒由包括一定重量配比的肉苁蓉种子、惰性添加物、粘化物和添加剂制成,其能减少播种量,提高寄生率;所述制备工艺通过将肉苁蓉种子进行预处理,提高种子净度和整齐度,然后将其与惰性添加物、粘化物和添加剂混合制备成种子颗粒,制备方法简单,条件易控,成本低廉。A fast-germinating Cistanche seed granule and a preparation process thereof. The invention discloses a Cistanche seed granule, a preparation process and a planting method of Cistanche. The Cistanche seed granule is made of Cistanche seeds in a certain weight ratio, inert additives, sticky substances and additives, which can reduce the amount of sowing, Improve the parasitism rate; the preparation process improves the cleanliness and uniformity of the seeds by pretreating the seeds of Cistanche deserticola, and then mixes them with inert additives, stickies and additives to prepare seed granules. The preparation method is simple and the conditions are easy to control. low cost.

Description

一种速萌肉苁蓉种子颗粒及其制备工艺A kind of fast-germinating cistanche seed granule and its preparation process

技术领域technical field

本发明属于干旱区药材种子加工及栽培领域,具体涉及一种速萌肉苁蓉种子颗粒及其制备工艺。The invention belongs to the field of medicinal seed processing and cultivation in arid regions, and in particular relates to a fast-germinating Cistanche seed granule and a preparation process thereof.

背景技术Background technique

肉苁蓉,俗称大芸,为列当科肉苁蓉属多年生专性寄生草本植物,分布于亚洲荒漠地带,寄生于梭梭、红柳、碱蓬等盐生、沙生植物的根部。Cistanche, commonly known as Dayun, is a perennial obligate parasitic herb belonging to the family Cistanche. It is distributed in the deserts of Asia and parasitizes the roots of saline and sandy plants such as Haloxylon Haloxylon, tamarisk, and Suaeda salsa.

现代药理研究表明,肉苁蓉具有提高性功能、抗衰老、提高学习记忆功能、抗老年痴呆症、通便等多方面的功能,素有“沙漠人参”之称,具有重要的经济和药用价值。受经济利益的驱使,肉苁蓉被不法分子地毯式掠夺性采挖,濒临灭绝,而且生态环境也遭到了严重破坏。Modern pharmacological research shows that Cistanche deserticola has many functions such as improving sexual function, anti-aging, improving learning and memory function, anti-senile dementia, and laxative. It is known as "desert ginseng" and has important economic and medicinal values. Driven by economic interests, Cistanche deserticola has been plundered by criminals in a carpet-like manner, and it is on the verge of extinction, and the ecological environment has also been severely damaged.

针对上述状况,需要开展肉苁蓉人工栽培,以提高其产量,同时促进生态环境治理。但在人工种植的过程中,由于肉苁蓉种子轻小圆滑,自然萌发困难,价格昂贵,造成其播种量较难控制、种植成本高、种子利用率低且寄生率不高,严重阻碍了人工接种繁殖。In response to the above situation, it is necessary to carry out artificial cultivation of Cistanche to increase its yield and promote ecological environment management. However, in the process of artificial planting, because Cistanche seeds are light, small and smooth, natural germination is difficult and expensive, resulting in difficult control of the seeding amount, high planting costs, low seed utilization rate and low parasitic rate, which seriously hinders artificial inoculation and reproduction. .

因此,有必要提供一种播种方便、萌发效率高、寄生率高、成本低廉的肉苁蓉种子颗粒及其制备工艺。Therefore, it is necessary to provide Cistanche deserticola seed granules with convenient sowing, high germination efficiency, high parasitic rate and low cost and a preparation process thereof.

发明内容Contents of the invention

为了克服上述问题,本发明人进行了锐意研究,结果发现:通过将肉苁蓉种子进行筛选、清选和精选处理,能够提高种子净度和一致性,而后将其与惰性添加物、粘化物混合制备成种子颗粒,能够降低播种量,同时,其添加的添加剂具有保水、促寄主生根和促萌发等功能,能够显著缩短寄生时间,提高寄生率,加速生长,从而完成了本发明。In order to overcome the above-mentioned problems, the present inventor has carried out intensive research and found that: by screening, cleaning and selecting the Cistanche seeds, the cleanliness and consistency of the seeds can be improved, and then mixed with inert additives and viscose Prepared into seed granules, the amount of sowing can be reduced, and at the same time, the added additives have functions such as water retention, rooting and germination promotion of the host, can significantly shorten the parasitic time, increase the parasitic rate, and accelerate growth, thereby completing the present invention.

具体来说,本发明的目的在于提供以下方面:Specifically, the object of the present invention is to provide the following aspects:

第一方面,提供了一种肉苁蓉种子颗粒,其中,所述种子颗粒由包括以下重量配比的组分制成:In the first aspect, a kind of cistanche seed granule is provided, wherein, the seed granule is made of components comprising the following weight ratio:

第二方面,提供了一种第一方面所述肉苁蓉种子颗粒的制备工艺,其中,所述工艺包括以下步骤:In a second aspect, there is provided a process for preparing Cistanche seed granules described in the first aspect, wherein the process comprises the following steps:

步骤1,种子预处理;Step 1, seed pretreatment;

步骤2,制备惰性添加物、粘化物及添加剂;Step 2, preparing inert additives, stickies and additives;

步骤3,制备肉苁蓉种子颗粒;Step 3, preparing Cistanche seed granules;

其中,步骤1中,所述预处理包括筛选和清选精选处理。Wherein, in step 1, the pretreatment includes screening and cleaning and refining.

第三方面,提供了一种第二方面所述制备工艺制得的肉苁蓉种子颗粒。The third aspect provides the Cistanche deserticola seed granules prepared by the preparation process described in the second aspect.

第四方面,提供了一种肉苁蓉的种植方法,优选采用第一方面所述的肉苁蓉种子颗粒进行种植,其中,所述种植方法包括以下步骤:In a fourth aspect, a planting method of Cistanche is provided, preferably using the Cistanche seed granules described in the first aspect for planting, wherein the planting method includes the following steps:

步骤I,选地整地,种植寄主;Step 1, selecting and preparing the land, planting the host;

步骤II,种植肉苁蓉;Step II, plant Cistanche;

步骤III,田间管理。Step III, field management.

本发明所具有的有益效果包括:The beneficial effects that the present invention has include:

(1)本发明所提供的肉苁蓉种子颗粒,增大了肉苁蓉种子的体积,能减少播种量4/5以上,播种方便,容易达到均匀一致,不间苗,节省大量劳力;(1) Cistanche seed granules provided by the present invention have increased the volume of Cistanche seeds, can reduce the seeding rate by more than 4/5, are convenient for sowing, are easy to achieve uniformity, do not thin out seedlings, and save a lot of labor;

(2)本发明所提供的肉苁蓉种子颗粒,抗压强度大,破碎率低,在湿润土壤中的裂解速度快;(2) Cistanche seed granules provided by the present invention have high compressive strength, low fragmentation rate, and fast cracking speed in moist soil;

(3)本发明所提供的肉苁蓉种子颗粒,提高了种子活力,缩短了寄生时间,促进了肉苁蓉种子萌发,提高了寄生率;(3) Cistanche seed granules provided by the present invention have improved seed vigor, shortened the parasitic time, promoted the germination of Cistanche seeds, and improved the parasitic rate;

(4)本发明所提供的肉苁蓉种子颗粒,具有蓄水、保水和供水的功能,能引导和促进寄主根系生长,加速宿主肉苁蓉的生长;(4) Cistanche seed granules provided by the present invention have the functions of water storage, water retention and water supply, can guide and promote the growth of the host root system, and accelerate the growth of the host Cistanche;

(5)本发明所提供的肉苁蓉种子颗粒,增加了种子的重量,便于机械播种;(5) Cistanche seed granules provided by the present invention have increased the weight of seeds and are convenient for mechanical sowing;

(6)本发明提供的肉苁蓉种子颗粒的制备工艺,方法简单,条件易控,试剂安全,成本低廉,适于规模化应用。(6) The preparation process of Cistanche deserticola seed granules provided by the present invention has the advantages of simple method, easy control of conditions, safe reagents, low cost, and is suitable for large-scale application.

附图说明Description of drawings

图1示出本发明一种优选实施方式的肉苁蓉种子颗粒结构示意图(包含1粒种子);Fig. 1 shows the schematic diagram of the particle structure of Cistanche deserticola seeds (comprising 1 seed) of a kind of preferred embodiment of the present invention;

图2示出本发明另一种优选实施方式的肉苁蓉种子颗粒结构示意图(包含多粒种子)。Fig. 2 shows another preferred embodiment of the present invention Cistanche seed particle structure schematic diagram (comprising multiple seeds).

具体实施方式Detailed ways

下面通过附图和实施例对本发明进一步详细说明。通过这些说明,本发明的特点和优点将变得更为清楚明确。The present invention will be further described in detail through the drawings and examples below. Through these descriptions, the features and advantages of the present invention will become more apparent.

第一方面,本发明提供了一种速萌肉苁蓉种子颗粒,其中,所述种子颗粒由包括以下重量配比的组分制成:In a first aspect, the present invention provides a kind of fast-germinating Cistanche seed granules, wherein the seed granules are made of components comprising the following weight ratios:

优选地,所述种子颗粒由包括以下重量配比的组分制成:Preferably, the seed particles are made of components comprising the following weight ratios:

更优选地,所述种子颗粒由包括以下重量配比的组分制成:More preferably, the seed particles are made of components comprising the following weight ratios:

本发明人研究发现,将肉苁蓉种子加工成丸粒状,增大了体积,能够有效减少播种量,且能够使播种均匀一致,节省劳动力。The inventors have found that processing the seeds of Cistanche deserticola into pellets increases the volume, can effectively reduce the amount of sowing, and can make the sowing uniform and save labor.

其中,所述肉苁蓉种子为荒漠肉苁蓉种子或管花肉苁蓉种子。Wherein, the Cistanche seeds are desert Cistanche seeds or Cistanche tuberosum seeds.

根据本发明一种优选的实施方式,所述惰性添加物选自高岭土、皂土、滑石粉、蛭石、铝矾土、农田潮土和漠土中的一种或多种。According to a preferred embodiment of the present invention, the inert additive is selected from one or more of kaolin, bentonite, talc, vermiculite, bauxite, farmland fluvo-aquic soil and desert soil.

在进一步优选的实施方式中,所述惰性添加物选自高岭土、皂土、蛭石、农田潮土和漠土中的一种或多种,优选为农田潮土和/或漠土。In a further preferred embodiment, the inert additive is selected from one or more of kaolin, bentonite, vermiculite, farmland fluvo-aquic soil and desert soil, preferably farmland fluvo-aquic soil and/or desert soil.

本发明人发现,选择农田潮土或漠土作为惰性添加物,可做到就地取材,且成本低廉、缓冲性好。The inventors found that choosing farmland fluvo-aquic soil or desert soil as an inert additive can achieve local materials with low cost and good cushioning properties.

根据本发明一种优选的实施方式,所述粘化物选自聚乙烯醇、聚醋酸乙烯、甲基纤维素、羧甲基纤维素、羟丙基纤维素、淀粉、凹凸棒矿石和红粘土中的一种或多种。According to a preferred embodiment of the present invention, the viscose is selected from polyvinyl alcohol, polyvinyl acetate, methyl cellulose, carboxymethyl cellulose, hydroxypropyl cellulose, starch, attapulgite ore and red clay one or more of .

在本发明中,所述粘化物具有优良的水溶性,且对种子萌发无副作用,在制作种子颗粒的过程中添加粘化物能够有效提高丸粒的抗压强度,减少破碎率。In the present invention, the sticky substance has excellent water solubility and has no side effects on seed germination, adding the sticky substance in the process of making seed granules can effectively improve the compressive strength of the pellets and reduce the breakage rate.

本发明人研究发现,基于0.5重量份的肉苁蓉种子,当加入的粘化物为0.02~0.09重量份,优选0.03~0.08重量份,更优选为0.05重量份时,制备的肉苁蓉种子颗粒的抗压强度较大、破碎率较低。当加入的粘化物小于0.02重量份时,制备的颗粒的抗压强度不高,容易发生破损;当加入的粘化物大于0.09重量份时,种子的抗压强度提高,裂解率降低,但是萌发率会降低。The inventors have found that, based on 0.5 parts by weight of Cistanche seeds, when the added viscose is 0.02 to 0.09 parts by weight, preferably 0.03 to 0.08 parts by weight, and more preferably 0.05 parts by weight, the compressive strength of the prepared Cistanche seeds granules Larger and less broken. When the sticky substance added was less than 0.02 parts by weight, the compressive strength of the prepared particles was not high, and damage occurred easily; when the sticky substance added was greater than 0.09 parts by weight, the compressive strength of the seeds increased, and the cracking rate decreased, but the germination rate will decrease.

在进一步优选的实施方式中,所述粘化物选自聚乙烯醇、聚醋酸乙烯、甲基纤维素、羧甲基纤维素、凹凸棒矿石和红粘土中的一种或多种。In a further preferred embodiment, the sticky substance is selected from one or more of polyvinyl alcohol, polyvinyl acetate, methylcellulose, carboxymethylcellulose, attapulgite and red clay.

在更进一步优选的实施方式中,所述粘化物为凹凸棒矿石和/或红粘土。In a further preferred embodiment, the viscose is attapulgite ore and/or red clay.

本发明人发现,凹凸棒矿石和红粘土是北方地区成本低廉、就地取材的矿物材料,具有质地细腻、保水性好、吸附性好、无毒无副作用、粘结性好及缓冲性好的优点,因此,本发明中优选选用凹凸棒矿石和/或红粘土作为粘化物。The inventors found that attapulgite ore and red clay are mineral materials with low cost and local materials in the northern region, which have fine texture, good water retention, good adsorption, non-toxic and no side effects, good cohesiveness and good cushioning Advantages, therefore, in the present invention, attapulgite and/or red clay are preferably selected as the viscose.

根据本发明一种优选的实施方式,所述添加剂包括保水剂、促生根剂和抗逆因子,三者的重量比为0.5:(0.003~0.009):(0.005~0.012),优选地,三者的重量比为0.5:(0.004~0.008):(0.006~0.01)。According to a preferred embodiment of the present invention, the additive includes a water-retaining agent, a root-promoting agent and an anti-stress factor, and the weight ratio of the three is 0.5: (0.003-0.009): (0.005-0.012), preferably, the three The weight ratio is 0.5:(0.004~0.008):(0.006~0.01).

根据本发明一种优选的实施方式中,所述保水剂为聚丙烯酰胺、聚丙烯酸钠或淀粉接枝丙烯酸盐中的一种或多种。According to a preferred embodiment of the present invention, the water retaining agent is one or more of polyacrylamide, sodium polyacrylate or starch grafted acrylate.

在本发明中,添加保水剂能吸收且保持自身重量百倍甚至上千倍的水,从而有效解决丸粒化种子在萌发过程中遇到的土壤干旱问题,提高种子萌发率。In the present invention, the addition of the water-retaining agent can absorb and maintain hundreds or even thousands of times of its own weight in water, thereby effectively solving the problem of soil drought encountered in the germination process of pelleted seeds and improving the germination rate of the seeds.

在进一步优选的实施方式中,所述保水剂为淀粉接枝丙烯酸盐,如淀粉接枝丙烯酸钠。In a further preferred embodiment, the water retaining agent is starch grafted acrylate, such as sodium starch grafted acrylate.

根据本发明另一种优选的实施方式,所述促生根剂为萘乙酸、吲哚乙酸、吲哚丁酸、萘氧乙酸和苯酚化合物中的一种或多种。According to another preferred embodiment of the present invention, the root-promoting agent is one or more of naphthaleneacetic acid, indoleacetic acid, indolebutyric acid, naphthyloxyacetic acid and phenolic compounds.

在进一步优选的实施方式中,所述促生根剂为萘乙酸、吲哚乙酸和萘氧乙酸中的一种或多种。In a further preferred embodiment, the root-promoting agent is one or more of naphthaleneacetic acid, indoleacetic acid and naphthyloxyacetic acid.

在更进一步优选的实施方式中,所述促生根剂为萘乙酸。In a still further preferred embodiment, the root-promoting agent is naphthaleneacetic acid.

其中,在种子颗粒中加入萘乙酸,能够促进寄主根系的生长,从而提高肉苁蓉种子的寄生率。Among them, adding naphthaleneacetic acid to the seed particles can promote the growth of the host root system, thereby increasing the parasitic rate of Cistanche deserticola seeds.

根据本发明另一种优选的实施方式,所述促生根剂包括以下重量配比的组分:According to another preferred embodiment of the present invention, the root-promoting agent comprises the following components by weight:

在进一步优选的实施方式中,所述促生根剂包括以下重量配比的组分:In a further preferred embodiment, the root-promoting agent comprises the following components in weight ratio:

在更进一步优选的实施方式中,所述促生根剂包括以下重量配比的组分:In a further preferred embodiment, the root-promoting agent comprises the following components by weight:

其中,萘乙酸和芸苔素内酯具有增加植物根系的功能,胺鲜酯能够提高植物过氧化物酶和硝酸还原酶的活性,促进植物细胞的分裂和伸长,促进根系的发育,调节植物体内养分的平衡,上述三个成分按照特定比例混合,能够较好地促进寄主根系的增殖和生长,从而进一步提高肉苁蓉的寄生率和萌发率。Among them, naphthaleneacetic acid and brassinolide have the function of increasing the root system of plants, diethyl diethyl hexanoate can increase the activity of plant peroxidase and nitrate reductase, promote the division and elongation of plant cells, promote the development of root system, and regulate the activity of plant cells. The balance of nutrients, the above three components mixed according to a specific ratio, can better promote the proliferation and growth of the host root system, thereby further increasing the parasitic rate and germination rate of Cistanche deserticola.

根据本发明一种优选的实施方式,所述复合菌由侧孢芽孢杆菌、纳豆芽孢杆菌、光合细菌和放线菌的菌粉复合而成。According to a preferred embodiment of the present invention, the composite bacteria are composed of bacterial powders of Bacillus lateralis, Bacillus natto, photosynthetic bacteria and actinomycetes.

在进一步优选的实施方式中,所述侧孢芽孢杆菌、纳豆芽孢杆菌、光合细菌和放线菌的重量比为1:(1.5~2.5):(0.5~1.5):(2~4),优选为1:(1.8~2.2):(0.7~1.3):(2.5~3.5),更优选为1:2:1:3。In a further preferred embodiment, the weight ratio of the Bacillus lateralis, Bacillus natto, photosynthetic bacteria and actinomycetes is 1: (1.5-2.5): (0.5-1.5): (2-4), Preferably it is 1:(1.8-2.2):(0.7-1.3):(2.5-3.5), more preferably 1:2:1:3.

其中,在使用过程中,添加剂中的有效活菌数≥2×108cfu/g。Wherein, during use, the number of effective viable bacteria in the additive is ≥2×10 8 cfu/g.

本发明人研究发现,通过在肉苁蓉种子颗粒的促生根剂中添加复合菌,能够显著促进寄主根系的生长,使得侧根不定根发生多,发根快,且能够促进和维持根系活性,提高寄主的抗逆境能力;同时,通过添加复合菌,能够刺激寄主根系分泌代谢产物,从而加速肉苁蓉种子的萌发和吸器的形成,提高萌发率和寄生率。The inventors have found that by adding compound bacteria to the root-promoting agent of Cistanche seed granules, the growth of the host root system can be significantly promoted, so that the adventitious roots of lateral roots are more, the roots are fast, and the activity of the root system can be promoted and maintained, and the resistance of the host can be improved. Adversity ability; at the same time, by adding complex bacteria, it can stimulate the host root system to secrete metabolites, thereby accelerating the germination of Cistanche seeds and the formation of haustoria, and improving the germination rate and parasitism rate.

根据本发明一种优选的实施方式,所述抗逆因子选自赤霉素、五氨基乙酰丙酸、壳聚糖、水杨酸和亚硝基铁氰化钠中的一种或多种。According to a preferred embodiment of the present invention, the stress resistance factor is selected from one or more of gibberellin, pentaaminolevulinic acid, chitosan, salicylic acid and sodium nitroferricyanide.

其中,所述抗逆因子主要用于提高种子萌发对冷害、盐碱等逆境的耐受性,包括植物激素或具有植物激素特性的化学物质。Wherein, the stress resistance factor is mainly used to improve the tolerance of seed germination to cold damage, saline-alkali and other adversities, including phytohormones or chemical substances with phytohormone properties.

在进一步优选的实施方式中,所述抗逆因子选自赤霉素、五氨基乙酰丙酸、壳聚糖和水杨酸中的一种或多种,优选为水杨酸。In a further preferred embodiment, the stress resistance factor is selected from one or more of gibberellin, pentaminolevulinic acid, chitosan and salicylic acid, preferably salicylic acid.

本发明人研究发现,水杨酸能够促进肉苁蓉种子的萌发和提高肉苁蓉中的抗逆性,从而能够显著提高肉苁蓉种子的萌发率。The inventors found that salicylic acid can promote the germination of Cistanche seeds and improve the stress resistance in Cistanche, thereby significantly increasing the germination rate of Cistanche seeds.

根据本发明一种优选的实施方式,所述添加剂还包括杀菌剂,基于0.5份保水剂的重量,所述杀菌剂的重量为0.005~0.009份,优选为0.006~0.008份。According to a preferred embodiment of the present invention, the additive further includes a bactericide, and based on the weight of 0.5 part of the water-retaining agent, the weight of the bactericide is 0.005-0.009 parts, preferably 0.006-0.008 parts.

在进一步优选的实施方式中,所述杀菌剂选自戊唑醇、己唑醇、苯醚甲环唑、丙环唑和三唑酮中的一种或多种。In a further preferred embodiment, the bactericide is selected from one or more of tebuconazole, hexaconazole, difenoconazole, propiconazole and triadimefon.

在更进一步优选的实施方式中,所述杀菌剂选自戊唑醇、己唑醇和三唑酮中的一种或多种,优选为戊唑醇。In a further preferred embodiment, the fungicide is selected from one or more of tebuconazole, hexaconazole and triadimefon, preferably tebuconazole.

第二方面,本发明还提供了一种速萌肉苁蓉种子颗粒的制备工艺,所述工艺包括以下步骤:In a second aspect, the present invention also provides a preparation process for fast-germinating Cistanche deserticola seed granules, said process comprising the following steps:

步骤1,种子预处理;Step 1, seed pretreatment;

步骤2,制备惰性添加物、粘化物及添加剂;Step 2, preparing inert additives, stickies and additives;

步骤3,制备肉苁蓉种子颗粒。Step 3, preparing Cistanche seed granules.

以下进一步描述本发明所述制备工艺:The preparation process of the present invention is further described below:

步骤1,种子预处理。Step 1, seed pretreatment.

其中,所述预处理包括对种子进行筛选、清选和精选处理。Wherein, the pretreatment includes screening, cleaning and refining the seeds.

根据本发明一种优选的实施方式,所述种子筛选包括依次用大筛和小筛进行筛选,According to a preferred embodiment of the present invention, the seed screening includes sequentially screening with a large sieve and a small sieve,

其中,所述大筛的孔径为0.6~1.4mm,优选为0.8~1.2mm,如1.0mm,和/或Wherein, the aperture of the large sieve is 0.6-1.4 mm, preferably 0.8-1.2 mm, such as 1.0 mm, and/or

所述小筛的孔径为0.4~0.8mm,优选为0.5~0.7mm,如0.6mm。The aperture of the small sieve is 0.4-0.8 mm, preferably 0.5-0.7 mm, such as 0.6 mm.

在进一步优选的实施方式中,在筛选后,优选采用小型螺旋塔对种子进行清选和精选。In a further preferred embodiment, after screening, the seeds are preferably cleaned and refined using a small spiral tower.

在本发明中,经过上述的清选精选,选取得到饱满、整齐、健壮的肉苁蓉种子。In the present invention, through the above-mentioned cleaning and selection, the plump, neat and robust Cistanche seeds are selected.

步骤2,制备惰性添加物、粘化物及添加剂。Step 2, preparing inert additives, stickies and additives.

根据本发明一种优选的实施方式,所述惰性添加物选自高岭土、皂土、滑石粉、蛭石、铝矾土、农田潮土和漠土中的一种或多种。According to a preferred embodiment of the present invention, the inert additive is selected from one or more of kaolin, bentonite, talc, vermiculite, bauxite, farmland fluvo-aquic soil and desert soil.

在进一步优选的实施方式中,所述惰性添加物选自高岭土、皂土、蛭石、农田潮土和漠土中的一种或多种,优选为农田潮土和/或漠土。In a further preferred embodiment, the inert additive is selected from one or more of kaolin, bentonite, vermiculite, farmland fluvo-aquic soil and desert soil, preferably farmland fluvo-aquic soil and/or desert soil.

根据本发明一种优选的实施方式,所述粘化物选自聚乙烯醇、聚醋酸乙烯、甲基纤维素、羧甲基纤维素、羟丙基纤维素、淀粉、凹凸棒矿石和红粘土中的一种或多种。According to a preferred embodiment of the present invention, the viscose is selected from polyvinyl alcohol, polyvinyl acetate, methyl cellulose, carboxymethyl cellulose, hydroxypropyl cellulose, starch, attapulgite ore and red clay one or more of .

在进一步优选的实施方式中,所述粘化物选自聚乙烯醇、聚醋酸乙烯、甲基纤维素、羧甲基纤维素、凹凸棒矿石和红粘土中的一种或多种。In a further preferred embodiment, the sticky substance is selected from one or more of polyvinyl alcohol, polyvinyl acetate, methylcellulose, carboxymethylcellulose, attapulgite and red clay.

在更进一步优选的实施方式中,所述粘化物为凹凸棒矿石和/或红粘土。In a further preferred embodiment, the viscose is attapulgite ore and/or red clay.

根据本发明一种优选的实施方式,称量惰性添加物和粘化物,然后进行粉碎和过筛处理。According to a preferred embodiment of the present invention, the inert additives and viscose are weighed, and then pulverized and sieved.

其中,在粉碎前优选将惰性添加物和粘化物晒干。Among them, it is preferable to dry the inert additives and stickies before pulverization.

在进一步优选的实施方式中,所述过筛为过100~150目筛,优选为110~130目筛,更优选为过120目筛。In a further preferred embodiment, the sieving is through a 100-150 mesh sieve, preferably a 110-130 mesh sieve, more preferably a 120 mesh sieve.

在更进一步优选的实施方式中,所述惰性添加物和粘化物的重量比为(0.8~1.2):(0.02~0.09),优选为(0.9~1.1):(0.03~0.08),更优选为1:(0.03~0.08),如1:0.05。In a further preferred embodiment, the weight ratio of the inert additive to the sticky substance is (0.8-1.2): (0.02-0.09), preferably (0.9-1.1): (0.03-0.08), more preferably 1: (0.03~0.08), such as 1:0.05.

其中,将粉碎过筛后的惰性添加物和粘化物按比例混匀备用。Wherein, the crushed and sieved inert additives and sticky substances are mixed in proportion and ready for use.

根据本发明一种优选的实施方式,所述添加剂包括保水剂、促生根剂和抗逆因子,三者的重量比为0.5:(0.003~0.009):(0.005~0.012),优选地,三者的重量比为0.5:(0.004~0.008):(0.006~0.01)。According to a preferred embodiment of the present invention, the additive includes a water-retaining agent, a root-promoting agent and an anti-stress factor, and the weight ratio of the three is 0.5: (0.003-0.009): (0.005-0.012), preferably, the three The weight ratio is 0.5:(0.004~0.008):(0.006~0.01).

在进一步优选的实施方式中,所述保水剂为聚丙烯酰胺、聚丙烯酸钠和淀粉接枝丙烯酸盐中的一种或多种。In a further preferred embodiment, the water retaining agent is one or more of polyacrylamide, sodium polyacrylate and starch grafted acrylate.

根据本发明一种优选的实施方式,所述促生根剂为萘乙酸、吲哚乙酸和萘氧乙酸中的一种或多种。According to a preferred embodiment of the present invention, the root-promoting agent is one or more of naphthaleneacetic acid, indoleacetic acid and naphthyloxyacetic acid.

在更进一步优选的实施方式中,所述促生根剂为萘乙酸。In a still further preferred embodiment, the root-promoting agent is naphthaleneacetic acid.

根据本发明另一种优选的实施方式,所述促生根剂包括以下重量配比的组分:According to another preferred embodiment of the present invention, the root-promoting agent comprises the following components by weight:

在进一步优选的实施方式中,所述促生根剂包括以下重量配比的组分:In a further preferred embodiment, the root-promoting agent comprises the following components in weight ratio:

在更进一步优选的实施方式中,所述促生根剂包括以下重量配比的组分:In a further preferred embodiment, the root-promoting agent comprises the following components by weight:

根据本发明一种优选的实施方式,所述复合菌由侧孢芽孢杆菌、纳豆芽孢杆菌、光合细菌和放线菌的菌粉复合而成。According to a preferred embodiment of the present invention, the composite bacteria are composed of bacterial powders of Bacillus lateralis, Bacillus natto, photosynthetic bacteria and actinomycetes.

在进一步优选的实施方式中,所述侧孢芽孢杆菌、纳豆芽孢杆菌、光合细菌和放线菌的重量比为1:(1.5~2.5):(0.5~1.5):(2~4),优选为1:(1.8~2.2):(0.7~1.3):(2.5~3.5),更优选为1:2:1:3。In a further preferred embodiment, the weight ratio of the Bacillus lateralis, Bacillus natto, photosynthetic bacteria and actinomycetes is 1: (1.5-2.5): (0.5-1.5): (2-4), Preferably it is 1:(1.8-2.2):(0.7-1.3):(2.5-3.5), more preferably 1:2:1:3.

根据本发明一种优选的实施方式,所述抗逆因子选自赤霉素、五氨基乙酰丙酸、壳聚糖、水杨酸和亚硝基铁氰化钠中的一种或多种。According to a preferred embodiment of the present invention, the stress resistance factor is selected from one or more of gibberellin, pentaaminolevulinic acid, chitosan, salicylic acid and sodium nitroferricyanide.

根据本发明一种优选的实施方式,所述添加剂还包括杀菌剂,基于0.5份保水剂的重量,所述杀菌剂的重量为0.005~0.009份,优选为0.006~0.008份。According to a preferred embodiment of the present invention, the additive further includes a bactericide, and based on the weight of 0.5 part of the water-retaining agent, the weight of the bactericide is 0.005-0.009 parts, preferably 0.006-0.008 parts.

在进一步优选的实施方式中,所述杀菌剂选自戊唑醇、己唑醇、苯醚甲环唑、丙环唑和三唑酮中的一种或多种。In a further preferred embodiment, the bactericide is selected from one or more of tebuconazole, hexaconazole, difenoconazole, propiconazole and triadimefon.

在更进一步优选的实施方式中,所述杀菌剂选自戊唑醇、己唑醇和三唑酮中的一种或多种,优选为戊唑醇。In a further preferred embodiment, the fungicide is selected from one or more of tebuconazole, hexaconazole and triadimefon, preferably tebuconazole.

根据本发明一种优选的实施方式,所述添加剂以溶液喷雾的形式施加,According to a preferred embodiment of the present invention, the additive is applied in the form of a solution spray,

所述添加剂的制备步骤如下:按照添加剂中各组分的溶解性,先溶解促生根剂,加水稀释;再将抗逆因子溶解,加入稀释后的促生根剂溶液;然后依次加入保水剂和杀菌剂,制备得到添加剂溶液。The preparation steps of the additive are as follows: according to the solubility of each component in the additive, first dissolve the root-promoting agent, add water to dilute; then dissolve the anti-stress factor, add the diluted root-promoting agent solution; then add the water-retaining agent and bactericidal agent to prepare an additive solution.

在进一步优选的实施方式中,制得的添加剂溶液的溶质中保水剂、促生根剂、抗逆因子和杀菌剂的含量比为0.5:(0.003~0.009):(0.005~0.012):(0.005~0.009),优选为(0.2~0.5):(0.004~0.008):(0.006~0.01):(0.006~0.008)。In a further preferred embodiment, the content ratio of water-retaining agent, root-promoting agent, stress resistance factor and bactericide in the solute of the prepared additive solution is 0.5: (0.003~0.009): (0.005~0.012): (0.005~0.012): 0.009), preferably (0.2-0.5): (0.004-0.008): (0.006-0.01): (0.006-0.008).

在更进一步优选的实施方式中,将制备好的添加剂溶液静置1~2小时备用。In a further preferred embodiment, the prepared additive solution is left to stand for 1-2 hours for later use.

步骤3,制备肉苁蓉种子颗粒。Step 3, preparing Cistanche seed granules.

其中,步骤3包括以下子步骤:Wherein, step 3 includes the following sub-steps:

步骤3-1,称量肉苁蓉种子、惰性添加物和粘化物,混匀后加入丸粒化装置的盛料器。Step 3-1: Weigh Cistanche seeds, inert additives and viscose, mix well and add to the feeder of the pelletizing device.

其中,加入的肉苁蓉种子、惰性添加物和粘化物的重量比为0.5:(0.8~1.2):(0.02~0.09),优选为0.5:(0.9~1.1):(0.03~0.08),更优选为0.5:1:0.05。Wherein, the weight ratio of the cistanche seeds added, the inert additives and the viscose is 0.5: (0.8~1.2): (0.02~0.09), preferably 0.5: (0.9~1.1): (0.03~0.08), more preferably 0.5:1:0.05.

步骤3-2,将添加剂雾化,然后进行丸粒化处理。In step 3-2, the additive is atomized and then pelletized.

将步骤2中制备得到的添加剂溶液装入喷雾装备中,待开启丸粒化装置后,将其雾化,均匀喷洒至种子上。Put the additive solution prepared in step 2 into the spray equipment, atomize it after turning on the pelletizing device, and spray it evenly on the seeds.

根据本发明一种优选的实施方式,所述丸粒化的转速为50~80转/分,丸粒化时间为10~20min。According to a preferred embodiment of the present invention, the rotational speed of the pelletization is 50-80 rpm, and the pelletization time is 10-20 minutes.

在进一步优选的实施方式中,所述肉苁蓉种子经丸粒化后粒径达到3~5mm。In a further preferred embodiment, the pelletized Cistanche seeds have a particle size of 3-5 mm.

步骤3-3,将丸粒化后的种子颗粒进行造型和干燥处理。Step 3-3, shaping and drying the pelletized seed particles.

其中,如图1和2所示,将制备的肉苁蓉种子颗粒进行造型,压制成球形,每个颗粒中包含1粒或多粒种子。Wherein, as shown in Figures 1 and 2, the prepared Cistanche seed granules are shaped and pressed into a spherical shape, and each granule contains one or more seeds.

根据本发明一种优选的实施方式,所述干燥处理为在35~40℃下干燥或在通风干燥处晾干,According to a preferred embodiment of the present invention, the drying treatment is drying at 35-40°C or drying in a ventilated and dry place,

所述干燥后种子颗粒的含水量在12%以下。The water content of the dried seed particles is below 12%.

任选地,将干燥后的种子颗粒进行裂解率、抗压强度、均匀性和有种子率检验,合格后进行包装。Optionally, the dried seed particles are inspected for cracking rate, compressive strength, uniformity and seed content rate, and are packaged after passing the test.

第三方面,本发明提供了一种根据第二方面所述制备工艺制得的肉苁蓉种子颗粒。In a third aspect, the present invention provides a Cistanche seed granule prepared according to the preparation process described in the second aspect.

第四方面,本发明提供了一种肉苁蓉的种植方法,优选采用第一方面所述的肉苁蓉种子颗粒进行种植,所述种植方法包括以下步骤:In a fourth aspect, the present invention provides a method for planting Cistanche, preferably using the Cistanche seed granules described in the first aspect for planting, the planting method comprising the following steps:

步骤I,选地整地,种植寄主。Step 1, choose the ground and prepare the ground, plant the host.

在4~5月,选择地势平坦、通透性强的沙壤土或风沙土地区种植寄主,优选采用开沟条播法种植寄主。From April to May, choose sandy loam or windy sandy soil with flat terrain and strong permeability to plant hosts, preferably using ditch drill method to plant hosts.

根据本发明一种优选的实施方式,所述寄主为梭梭树、红柳或柽柳,优选为梭梭树和/或柽柳。According to a preferred embodiment of the present invention, the host is Haloxylon, tamarisk or Tamarix, preferably Haloxylon and/or Tamarix.

在进一步优选的实施方式中,所述种植的寄主的间距不超过1.5m,行距不超过4m。In a further preferred embodiment, the distance between the planted hosts is no more than 1.5m, and the row spacing is no more than 4m.

在更进一步优选的实施方式中,所述寄主的种植密度大于130株/亩。In a further preferred embodiment, the planting density of the host is greater than 130 plants/mu.

根据本发明一种优选的实施方式,在种植寄主前,先对种植区域进行施肥处理,所述施加的肥料包括以下重量配比的组分:According to a preferred embodiment of the present invention, before the host is planted, the planting area is fertilized, and the applied fertilizer includes the following components by weight:

尿素 15份15 parts of urea

磷二胺 2~6份Phosphorus diamine 2 to 6 parts

黄腐酸浓缩液 5~15份。Fulvic acid concentrate 5-15 parts.

优选地,述施加的肥料包括以下重量配比的组分:Preferably, the fertilizer applied comprises the following components by weight:

尿素 15份15 parts of urea

磷二胺 3~5份Phosphorus diamine 3~5 parts

黄腐酸浓缩液 6~13份。6-13 parts of fulvic acid concentrate.

本发明人发现,黄腐酸由于含有羟基、酚羟基等官能团,有较强的络合、螯合和表面吸附能力,能减少铵态氮的损失;能够增加磷在土壤中移动距离,抑制土壤对水溶性磷的固定,使无效磷转化为有效磷,促进根系对磷的吸收;黄腐酸还可以吸收存储钾离子,提高有效钾的含量,对钾肥增效明显。The inventors have found that fulvic acid has strong complexation, chelation and surface adsorption capacity due to containing functional groups such as hydroxyl and phenolic hydroxyl, and can reduce the loss of ammonium nitrogen; it can increase the moving distance of phosphorus in the soil, and inhibit the impact of soil on the soil. The fixation of water-soluble phosphorus converts ineffective phosphorus into effective phosphorus, and promotes the absorption of phosphorus by the root system; fulvic acid can also absorb and store potassium ions, increase the content of available potassium, and significantly increase the efficiency of potassium fertilizer.

在进一步优选的实施方式中,所述肥料的施加量为7~13kg/亩,优选为10kg/亩。In a further preferred embodiment, the application amount of the fertilizer is 7-13 kg/mu, preferably 10 kg/mu.

步骤II,种植肉苁蓉。Step II, plant Cistanche deserticola.

根据本发明一种优选的实施方式,在寄主种植两年或三年后,种植肉苁蓉,所述种植时间为春季或秋季。According to a preferred embodiment of the present invention, Cistanche deserticola is planted two or three years after the host is planted, and the planting time is spring or autumn.

在进一步优选的实施方式中,在寄主主干的两侧40~50cm处开沟,用于播种制备的丸粒化肉苁蓉种子颗粒。In a further preferred embodiment, trenches are dug at 40-50 cm on both sides of the host trunk for sowing the prepared pelleted Cistanche deserticola seed particles.

本发明人研究发现,寄主主干两侧40~50cm处为根系发达处,于此处播种,有利于提高肉苁蓉的寄生率。The inventors have found that the 40-50 cm on both sides of the main trunk of the host is a well-developed root system, and sowing seeds here is beneficial to improving the parasitism rate of Cistanche deserticola.

在更进一步优选的实施方式中,所述肉苁蓉种子颗粒的播种量为5-10粒/米。In a further preferred embodiment, the sowing rate of the Cistanche deserticola seed granules is 5-10 grains/m.

其中,上述播种的肉苁蓉种子颗粒中包含1-3粒肉苁蓉种子。Wherein, the sown Cistanche seed granules include 1-3 Cistanche seeds.

步骤III,田间管理。Step III, field management.

在本发明中,在播种后的每年春夏季,优选在寄主接种沟内视墒情滴水2-3次。In the present invention, in the spring and summer of each year after sowing, it is preferred to drip water 2-3 times in the host inoculation ditch depending on the moisture content.

其中,在肉苁蓉生长过程中,清除田间杂草,采用物理和化学相结合的方式,防治梭梭病虫害,以保证肉苁蓉的品质。Among them, during the growth of Cistanche deserticola, field weeds are removed, and physical and chemical methods are used to prevent and control Haloxylon plant diseases and insect pests, so as to ensure the quality of Cistanche deserticola.

实施例Example

以下通过具体实例进一步描述本发明,不过这些实例仅仅是范例性的,并不对本发明的保护范围构成任何限制。The present invention is further described below through specific examples, but these examples are only exemplary and do not constitute any limitation to the protection scope of the present invention.

实施例1Example 1

按照以下步骤制备肉苁蓉种子颗粒:Follow the steps below to prepare Cistanche seed granules:

(1)清选精选种子:将收集的荒漠肉苁蓉种子,分别过1.0mm的大筛和0.6mm的小筛,然后用小型螺旋塔对种子进行精选,获取饱满、整齐、健壮的净种子;(1) Cleaning and selecting seeds: Pass the collected desert cistanche seeds through a large sieve of 1.0mm and a small sieve of 0.6mm, and then use a small spiral tower to select the seeds to obtain plump, neat and strong clean seeds ;

(2)将农田潮土晒干粉碎,然后过120目筛,将凹凸棒矿石晒干粉碎,然后过120目筛;(2) dry and pulverize farmland fluvo-aquic soil, then pass through a 120-mesh sieve, dry and pulverize the attapulgite ore, and then pass through a 120-mesh sieve;

将萘乙酸用丙酮溶解,加水稀释至浓度为0.004%,然后将水杨酸用丙酮溶解,加入稀释的萘乙酸溶液,使水杨酸的浓度为0.006%,再加入淀粉接枝丙烯酸钠和戊唑醇,使得淀粉接枝丙烯酸钠的浓度为0.5%,戊唑醇的浓度为0.006%;Dissolve naphthaleneacetic acid in acetone, add water to dilute to a concentration of 0.004%, then dissolve salicylic acid in acetone, add the diluted naphthaleneacetic acid solution to make the concentration of salicylic acid 0.006%, then add starch grafted sodium acrylate and valeric acid Tebuconazole, so that the concentration of starch grafted sodium acrylate is 0.5%, and the concentration of tebuconazole is 0.006%;

将制备好的上述溶液静置1~2小时,备用。The prepared above solution was left to stand for 1 to 2 hours, and set aside.

(3)按0.5:1:0.05的重量比依次称取0.5kg肉苁蓉种子、1kg粉碎过筛后的农田潮土和0.05kg粉碎过筛后的凹凸棒矿石,混匀后置于丸粒化机(型号为rh-480)中;(3) Weigh 0.5kg of cistanche seeds, 1kg of crushed and sieved farmland fluvo-aquic soil and 0.05kg of crushed and sieved attapulgite ore in sequence at a weight ratio of 0.5:1:0.05, and place them in a pelletizing machine after mixing. (Model is rh-480);

然后按肉苁蓉种子:添加剂为0.05:0.04的重量比量取添加剂溶液(其中添加剂的重量以溶质的重量计),置于丸粒化机的喷雾装备内,开启丸粒化机,调节转速至60转/分,丸粒化15min,使肉苁蓉种子丸粒化后的粒径为4mm;Then by Cistanche seeds: the additive is 0.05:0.04 weight ratio to measure the additive solution (wherein the weight of the additive is based on the weight of the solute), put it in the spray equipment of the pelletizing machine, open the pelletizing machine, and adjust the rotating speed to 60 RPM, pelletization 15min, the particle diameter after making the pelletization of Cistanche seeds is 4mm;

将制备的肉苁蓉种子颗粒压制成球形,然后于35℃下干燥至含水量为12%以下;pressing the prepared cistanche seed granules into a spherical shape, and then drying at 35°C until the water content is below 12%;

按照以下步骤种植肉苁蓉:Follow these steps to grow Cistanche:

(I)在第一年的4~5月,选择地势平坦的沙壤土地,按照10kg/亩的施肥量施加肥料(尿素、磷二胺和黄腐酸浓缩液的重量比为15:4:10),然后种植梭梭树,使得种植间距为1.3m,行距为3m,种植密度为140株/亩。(1) From April to May in the first year, select flat sandy soil, apply fertilizer according to the fertilization rate of 10kg/mu (the weight ratio of urea, phosphorus diamine and fulvic acid concentrate is 15:4:10 ), and then plant Haloxylon trees so that the planting spacing is 1.3m, the row spacing is 3m, and the planting density is 140 plants/mu.

(II)在种植梭梭树后的第三年的春天,在梭梭树主干的两侧45cm处开沟,播种上述制备的肉苁蓉种子颗粒,播种量为10粒/米(每粒种子颗粒中包含1粒肉苁蓉种子)。(II) in the spring of the third year after planting the Haloxylon tree, ditch at 45cm, both sides of the Haloxylon tree trunk, sow the above-mentioned prepared Cistanche seed granules, and the sowing rate is 10 grains/meter (in every seed granule) Contains 1 Cistanche seed).

(III)在肉苁蓉生长过程中,清除田间杂草,采用物理和化学相结合的方式,防治梭梭病虫害,以保证肉苁蓉的品质。(III) During the growth process of Cistanche deserticola, remove weeds in the field, and use a combination of physical and chemical methods to prevent and control Haloxylon saxaul diseases and insect pests, so as to ensure the quality of Cistanche deserticola.

实施例2Example 2

本实施例所用方法与实施例1相似,区别在于,The method used in this embodiment is similar to that of Example 1, the difference is that

步骤(2)中,采用农田漠土代替农田潮土;采用红粘土代替凹凸棒矿石;In step (2), farmland desert soil is used to replace farmland fluvo-aquic soil; red clay is used to replace attapulgite ore;

萘乙酸加水稀释至浓度为0.008%,水杨酸浓度为0.01%;Dilute naphthaleneacetic acid with water to a concentration of 0.008%, and salicylic acid to a concentration of 0.01%;

步骤(3)中,按0.5:1:0.08的重量比依次称取0.5kg肉苁蓉种子、1kg粉碎过筛后的农田漠土和0.08kg粉碎过筛后的红粘土。In step (3), 0.5 kg of cistanche seeds, 1 kg of crushed and sieved farmland desert soil and 0.08 kg of crushed and sieved red clay were sequentially weighed in a weight ratio of 0.5:1:0.08.

实施例3Example 3

本实施例所用方法与实施例1相似,区别在于,种子颗粒为管花肉苁蓉种子颗粒,寄主为两年生柽柳。The method used in this example is similar to Example 1, the difference is that the seed granules are Cistanche tuberose seed granules, and the host is Tamarisk biennial.

实施例4Example 4

本实施例所用方法与实施例2相似,区别在于,种子颗粒为管花肉苁蓉种子颗粒,寄主为两年生柽柳。The method used in this example is similar to Example 2, the difference is that the seed granules are the seed granules of Cistanche tubulosa, and the host is the biennial Tamarix.

实施例5Example 5

本实施例所用方法与实施例1相似,区别在于,步骤(3)中,粉碎过筛后的凹凸棒矿石的添加量为0.03kg。The method used in this embodiment is similar to that of embodiment 1, the difference is that in step (3), the amount of crushed and sieved attapulgite ore added is 0.03kg.

实施例6Example 6

本实施例所用方法与实施例1相似,区别在于,步骤(3)中,粉碎过筛后的凹凸棒矿石的添加量为0.08kg。The method used in this embodiment is similar to that of embodiment 1, the difference is that in step (3), the amount of attapulgite ore after crushing and sieving is 0.08kg.

实施例7Example 7

本实施例所用方法与实施例1相似,区别在于,促生根剂为萘乙酸、芸苔素内酯、胺鲜酯和复合菌的复合物(比例为10:0.7:2:3),添加浓度以萘乙酸的浓度计,其中复合菌中侧孢芽孢杆菌、纳豆芽孢杆菌、光合细菌和放线菌菌粉的重量比为1:2:1:3,添加剂中的有效活菌数为3×108cfu/g。The method used in this embodiment is similar to that of Example 1, the difference is that the root-promoting agent is a compound (ratio of 10:0.7:2:3) of naphthalene acetic acid, brassinolide, diethyl hexanoate and compound bacteria, and the added concentration In terms of the concentration of naphthaleneacetic acid, the weight ratio of Bacillus lateralis, Bacillus natto, photosynthetic bacteria and actinomycetes in the composite bacteria is 1:2:1:3, and the effective number of viable bacteria in the additive is 3 ×10 8 cfu/g.

实施例8Example 8

本实施例所用方法与实施例1相似,区别在于,促生根剂为萘乙酸、芸苔素内酯、胺鲜酯和复合菌的复合物(比例为10:0.5:3:2),添加浓度以萘乙酸的浓度计,其中复合菌中侧孢芽孢杆菌、纳豆芽孢杆菌、光合细菌和放线菌菌粉的重量比为1:2.5:1.5:2,添加剂中的有效活菌数为2×108cfu/g。The method used in this embodiment is similar to that of Example 1, the difference is that the root-promoting agent is a compound (ratio of 10:0.5:3:2) of naphthalene acetic acid, brassinolide, diethyl hexanoate and composite bacteria, and the added concentration Based on the concentration of naphthaleneacetic acid, the weight ratio of Bacillus lateralis, Bacillus natto, photosynthetic bacteria and actinomycetes in the composite bacteria is 1:2.5:1.5:2, and the effective number of viable bacteria in the additive is 2 ×10 8 cfu/g.

实施例9Example 9

本实施例所用方法与实施例1相似,区别在于,在种植梭梭树之前,施加的肥料中尿素、磷二胺、黄腐酸浓缩液的比为15:5:13,施加量为7kg/亩。The method used in this embodiment is similar to Example 1, the difference is that before planting Haloxylon tree, the ratio of urea, phosphorus diamine, fulvic acid concentrate in the applied fertilizer is 15:5:13, and the application amount is 7kg/ mu.

实施例10Example 10

本实施例所用方法与实施例1相似,区别在于,肉苁蓉种子颗粒播种在梭梭树主杆两侧50cm处。The method used in the present embodiment is similar to that of Example 1, the difference being that the Cistanche deserticola seed particles are sown at 50 cm on both sides of the Haloxylon main pole.

对比例comparative example

对比例1Comparative example 1

本对比例所用方法与实施例1相似,区别在于,制备肉苁蓉种子颗粒的过程中,惰性物质中不加入凹凸棒矿石、化学物质、激素和保水剂等添加剂,种子为管花肉苁蓉种子,寄主为柽柳;The method used in this comparative example is similar to Example 1, the difference is that in the process of preparing Cistanche seed granules, additives such as attapulgite ore, chemical substances, hormones and water-retaining agents are not added to the inert substances, the seeds are Cistanche tuberosum seeds, and the host is Tamarix;

播种量为20粒/米。The seeding rate is 20 grains/m.

对比例2Comparative example 2

本对比例所用方法与实施例1相似,区别在于,制备肉苁蓉种子颗粒的过程中,惰性物质中不加入凹凸棒矿石、化学物质、激素和保水剂等添加剂,种子为荒漠肉苁蓉种子,寄主为梭梭;The method used in this comparative example is similar to Example 1, the difference is that in the process of preparing Cistanche seed granules, additives such as attapulgite ore, chemical substances, hormones and water-retaining agents are not added to the inert substances, the seeds are desert Cistanche seeds, and the host is shuttle shuttle;

播种量为20粒/米。The seeding rate is 20 grains/m.

对比例3Comparative example 3

本对比例所播种的肉苁蓉种子为未经丸粒化的种子,其经过清选、精选处理后直接用于播种;The Cistanche seeds sown in this comparative example are seeds without pelleting, which are directly used for sowing after cleaning and selection;

播种量为100粒/米。The seeding rate is 100 grains/m.

对比例4Comparative example 4

本对比例所用方法与实施例7相似,区别在于,所述促生根剂中不添加复合菌。The method used in this comparative example is similar to that of Example 7, the difference is that no compound bacteria is added in the root-promoting agent.

实验例Experimental example

实验例1Experimental example 1

以实施例1~6和对比例1、2中制备的肉苁蓉种子颗粒为样品,采用全自动谷物硬度计(ST120B)测定种子颗粒的抗压强度;随机取100粒肉苁蓉种子颗粒,置于湿润纸床上,待崩解后逐丸检测种子的有种子率;统计肉苁蓉种子颗粒在一定压力强度(5N~10N)下的破碎率;随机取100粒肉苁蓉种子颗粒,浸泡在水中,统计1min内丸粒的裂解率,结果如表1所示。Taking the Cistanche seed granules prepared in Examples 1 to 6 and Comparative Examples 1 and 2 as samples, adopt a full-automatic grain hardness meter (ST120B) to measure the compressive strength of the seed granules; randomly get 100 Cistanche seed granules and place them on wet paper On the bed, after the disintegration, the seed rate of the seeds is detected pellet by pill; the crushing rate of the Cistanche seed granules under a certain pressure intensity (5N~10N) is counted; 100 Cistanche seed granules are randomly taken, soaked in water, and the pellets within 1min are counted. Cracking rate, the results are shown in Table 1.

表1不同丸粒化方式对肉苁蓉种子颗粒物理特性的影响Table 1 Effects of different pelleting methods on the physical properties of Cistanche seed granules

其中,有种子率=(供试种子颗粒总数-无种子的颗粒数)/供试种子颗粒总数×100%;Wherein, there is seed rate=(for testing seed particle total number-the particle number without seed)/for testing seed particle total number * 100%;

破碎率=破碎的种子颗粒数/供试种子颗粒总数×100%;Breakage rate = number of broken seed particles / total number of test seed particles × 100%;

裂解率=规定时间内裂解的种子颗粒数/供试种子颗粒总数×100%。Cracking rate = number of seed particles cracked within a specified time/total number of test seed particles × 100%.

由表1可知,本发明实施例中制备的肉苁蓉种子颗粒,其抗压强度和有种子率显著高于对比例1和对比例2中制备的种子颗粒;本发明实施例中制备的种子颗粒的破碎率均为0,说明在种子颗粒中加入粘化物能够保证种子颗粒在包装、运输及贮藏过程中的完整性。As can be seen from Table 1, the Cistanche seed granules prepared in the examples of the present invention have significantly higher compressive strength and seed percentage than the seed granules prepared in Comparative Example 1 and Comparative Example 2; the seed granules prepared in the examples of the present invention The breakage rate was 0, indicating that adding viscose to the seed granules could ensure the integrity of the seed granules during packaging, transportation and storage.

实验例2Experimental example 2

采用接种沟内随机取样点采挖的方法(在春季接种后,于11月中上旬,土壤封冻前,在接种沟内随机布点挖掘,仔细观察采挖点接种沟内肉苁蓉寄生情况,若寄主根部有肉苁蓉寄生且显著膨大,视为已经寄生,若无膨大或瘤状寄生物,视为无寄生),检测实施例1~10和对比例1~4中种植肉苁蓉的当年寄生率,结果如表2所示。Adopt the method of digging at random sampling points in the inoculation ditch (after inoculation in spring, in mid-to-early November, before the soil is frozen, dig at random points in the inoculation ditch, carefully observe the parasitism of Cistanche in the inoculation ditch at the digging point, if the host root Cistanche is parasitized and significantly enlarged, it is regarded as parasitic, if there is no enlarged or tumor-like parasite, it is regarded as no parasitic), the parasitic rate of planting Cistanche in the detection examples 1~10 and comparative examples 1~4, the results are shown in the table 2.

表2Table 2

其中,当年寄生率%=(当年寄生样点/供测试样点数)×100%。由表2可以看出,本发明实施例中的用种量显著少于对比例3中的用种量,实施例1~10中肉苁蓉的当年寄生率显著高于对比例1~4中的当年寄生率,说明本发明实施例所制备的肉苁蓉种子颗粒及肉苁蓉制备方法,能够减少用种量,缩短寄生时间,提高寄生率。Among them, the parasitic rate % of the year = (parasitic sample points in the current year/number of test sample points) × 100%. As can be seen from Table 2, the amount of seed used in the examples of the present invention is significantly less than that of comparative example 3, and the annual parasitic rate of Cistanche deserticola in Examples 1 to 10 is significantly higher than that in Comparative Examples 1 to 4. The parasitic rate shows that the prepared Cistanche seed granules and the Cistanche preparation method in the embodiments of the present invention can reduce the amount of seeds used, shorten the parasitic time, and improve the parasitic rate.

由实施例7和对比例4的当年寄生率结果可知,添加了复合菌的促生根剂能够显著提高肉苁蓉种子的当年寄生率,说明复合菌促进了寄主根系释放代谢产物,进而促进了肉苁蓉吸器的形成,提高了寄生率。From the results of the parasitic rate of the current year in Example 7 and Comparative Example 4, it can be seen that the root-promoting agent added with compound bacteria can significantly improve the parasitic rate of Cistanche seeds in the same year, indicating that the compound bacteria promote the release of metabolites from the host root system, and then promote the growth of the haustoria of Cistanche. formed, increasing the parasitic rate.

以上结合了优选的实施方式对本发明进行了说明,不过这些实施方式仅是范例性的,仅起到说明性的作用。在此基础上,可以对本发明进行多种替换和改进,这些均落入本发明的保护范围内。The present invention has been described above in conjunction with preferred embodiments, but these embodiments are only exemplary and serve as illustrations only. On this basis, various replacements and improvements can be made to the present invention, all of which fall within the protection scope of the present invention.

Claims (10)

1.一种肉苁蓉种子颗粒,其特征在于,所述种子颗粒由包括以下重量配比的组分制成:1. a kind of cistanche seed granule, is characterized in that, described seed granule is made of the component that comprises following weight ratio: 2.根据权利要求1所述的肉苁蓉种子颗粒,其特征在于,所述肉苁蓉种子为经过预处理的种子,所述预处理包括筛选、清选和精选处理。2. The Cistanche seed granule according to claim 1, characterized in that, the Cistanche seed is a pretreated seed, and the pretreatment includes screening, cleaning and fine-tuning. 3.根据权利要求1所述的肉苁蓉种子颗粒,其特征在于,所述惰性添加物选自高岭土、皂土、滑石粉、蛭石、铝矾土、农田潮土和漠土中的一种或多种;3. Cistanche seed granule according to claim 1, is characterized in that, described inert additive is selected from a kind of in kaolin, bentonite, talcum powder, vermiculite, bauxite, farmland fluvo-aquic soil and desert soil or various; 所述粘化物选自聚乙烯醇、聚醋酸乙烯、甲基纤维素、羧甲基纤维素、羟丙基纤维素、淀粉、凹凸棒矿石和红粘土中的一种或多种;和/或The viscose is selected from one or more of polyvinyl alcohol, polyvinyl acetate, methyl cellulose, carboxymethyl cellulose, hydroxypropyl cellulose, starch, attapulgite and red clay; and/or 所述添加剂包括保水剂、促生根剂和抗逆因子,三者的重量比为0.5:(0.003~0.009):(0.005~0.012)。The additive includes a water retaining agent, a root-promoting agent and an anti-stress factor, and the weight ratio of the three is 0.5:(0.003-0.009):(0.005-0.012). 4.根据权利要求3所述的肉苁蓉种子颗粒,其特征在于,所述促生根剂为萘乙酸、吲哚乙酸、吲哚丁酸、萘氧乙酸和苯酚化合物中的一种或多种。4. Cistanche seed granule according to claim 3, is characterized in that, described root-promoting agent is one or more in naphthalene acetic acid, indole acetic acid, indole butyric acid, naphthyloxy acetic acid and phenol compound. 5.根据权利要求3所述的肉苁蓉种子颗粒,其特征在于,所述促生根剂包括以下重量配比的组分:5. cistanche seed granule according to claim 3, is characterized in that, described root-promoting agent comprises the component of following weight ratio: 6.根据权利要求3所述的肉苁蓉种子颗粒,其特征在于,所述添加剂还包括杀菌剂,所述杀菌剂选自戊唑醇、己唑醇、苯醚甲环唑、丙环唑和三唑酮中的一种或多种。6. Cistanche seed granule according to claim 3, is characterized in that, described additive also comprises bactericide, and described bactericide is selected from tebuconazole, hexaconazole, difenoconazole, propiconazole and three One or more of oxazolones. 7.一种权利要求1至6之一所述肉苁蓉种子颗粒的制备工艺,其特征在于,所述工艺包括以下步骤:7. A preparation process of Cistanche seed granules according to one of claims 1 to 6, characterized in that, said process comprises the following steps: 步骤1,种子预处理;Step 1, seed pretreatment; 步骤2,制备惰性添加物、粘化物及添加剂;Step 2, preparing inert additives, stickies and additives; 步骤3,制备肉苁蓉种子颗粒;Step 3, preparing Cistanche seed granules; 其中,步骤1中,所述预处理包括筛选、清选和精选处理。Wherein, in step 1, the pretreatment includes screening, cleaning and fine-tuning. 8.根据权利要求7所述的制备工艺,其特征在于,步骤1中,所述种子筛选包括依次用大筛和小筛进行筛选,8. The preparation process according to claim 7, characterized in that, in step 1, the seed screening comprises sequentially screening with a large sieve and a small sieve, 其中,所述大筛的孔径为0.6~1.4mm,所述小筛的孔径为0.4~0.8mm。Wherein, the aperture of the large sieve is 0.6-1.4mm, and the aperture of the small sieve is 0.4-0.8mm. 9.一种权利要求7或8所述的制备工艺制得的肉苁蓉种子颗粒。9. the cistanche seed particle that a kind of preparation technology described in claim 7 or 8 makes. 10.一种肉苁蓉的种植方法,优选采用权利要求1至6之一所述的肉苁蓉种子颗粒进行种植,其特征在于,所述种植方法包括以下步骤:10. A planting method of Herba Cistanche, preferably planted with the seed particles of Herba Cistanche according to one of claims 1 to 6, characterized in that said planting method comprises the following steps: 步骤I,选地整地,种植寄主;Step 1, selecting and preparing the land, planting the host; 步骤II,种植肉苁蓉;Step II, plant Cistanche; 步骤III,田间管理。Step III, field management.
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