CN110220836B - Flow cytometry analyzer for in-vitro detection - Google Patents
Flow cytometry analyzer for in-vitro detection Download PDFInfo
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- CN110220836B CN110220836B CN201910562735.6A CN201910562735A CN110220836B CN 110220836 B CN110220836 B CN 110220836B CN 201910562735 A CN201910562735 A CN 201910562735A CN 110220836 B CN110220836 B CN 110220836B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
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Abstract
The invention relates to the technical field of cell analysis instruments, and discloses a flow cytometry for in-vitro detection, which comprises a base, wherein the left side of the upper surface of the base is fixedly connected with an organism, the middle part of the right side of the lower surface of the organism is fixedly connected with a cell sorter, the lower surface of the organism is fixedly connected with two limiting rods, the two limiting rods are respectively positioned at the two sides of the cell sorter and are mutually symmetrical by taking the central line of the cell sorter in the vertical direction as a symmetrical axis. This flow cytometry appearance for external detection through the annular swing joint mode between arc pole and the arc pipe, and convenient to use person limits the position of collector, through the joint connected mode between constant head tank and the vertical rod, and convenient to use person fixes the position of collector, and the elastic potential energy person of facilitating the use of T shaped plate through the elastic shrinkage part changes the collector, has improved this convenience that flow cytometry changes the collector for external detection.
Description
Technical Field
The invention relates to the technical field of cell analysis instruments, in particular to a flow cytometry analyzer for in-vitro detection.
Background
Outside human body, clinical diagnosis information is obtained by detecting human body samples, and then diseases or body functions are judged, namely, in-vitro detection, wherein the human body samples refer to blood, body fluid, tissues and the like; the in vitro diagnostic product is mainly composed of diagnostic instruments and diagnostic reagents, and the flow cytometer is a device for automatically analyzing and sorting cells, can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in a liquid, and sorts specified cell subsets according to a preselected parameter range.
When the existing flow cytometry is used for installing the collector, a user cannot conveniently fix the collector and the cell sorting device on the machine body, so that the time for replacing the collector by the user is increased, the replacement speed is slow, the existing flow cytometry is not convenient for installing and replacing the collectors of different models, the applicability is low, and therefore the flow cytometry for in vitro detection is provided.
Disclosure of Invention
The invention provides a flow cytometry for in vitro detection, which has the advantages of improving the convenience and the applicability of replacing a collector of the flow cytometry for in vitro detection and solving the problems in the background technology.
In order to achieve the above purpose, the invention provides the following technical scheme to realize: the utility model provides an external flow cytometry that detects uses analyzer, includes the base, the left side fixed connection organism of base upper surface, the middle part fixedly connected with cell sorter on organism lower surface right side, the lower fixed connection of surface of organism has the gag lever post, the quantity of gag lever post is two, two the gag lever post is located the both sides of cell sorter respectively and uses the central line of the vertical direction of cell sorter as symmetry axis mutual symmetry, the bottom of cell sorter is equipped with the collector, the right side fixedly connected with control panel of collector, the surface of collector is provided with the arc pipe.
Optionally, the right side of the upper surface of the base is fixedly connected with a fixing plate, the fixing plate is located under the collector, and the distance between the top of the fixing plate and the bottom of the collector is three centimeters.
Optionally, the number of the arc pipes is four, four the arc pipes are movably connected through arc rods between every two adjacent arc pipes, four the arc clamping plates are fixedly connected to one side of each arc pipe in the opposite direction, the anti-slip devices are fixedly connected to the outer sides of the arc pipes and are located on the left side and the right side of the collector respectively, and the arc plates are fixedly connected to the outer sides of the arc pipes and are located on the upper side and the lower side of the collector respectively.
Optionally, the bottom of the arc clamping plate is clamped with the back of the outer edge of the collector, the arc clamping plate is isosceles trapezoid-shaped, and the length of a connecting line between every two adjacent arc clamping plates is equal.
Optionally, the anti-skid device comprises a transverse plate, a transverse groove is formed in the front face of the transverse plate, and the inside of the transverse groove is movably connected with the outer surface of the limiting rod.
Optionally, the below of arc is equipped with T-shaped plate, the top of T-shaped plate runs through arc and barrel in proper order and extends to the inside of barrel, the surface of T-shaped plate respectively with the middle part of arc and the bottom swing joint of barrel.
Optionally, the bottom fixedly connected with barrel of organism, the limiting plate has been cup jointed in the inside activity of barrel, the outside surface swing joint of the inside of limiting plate and T shaped plate, the inside fixedly connected with fixed column of barrel.
Optionally, the bottom of the fixing column is movably sleeved with the top of the T-shaped plate, an elastic shrinkage component is movably sleeved on the outer surface of the fixing column, and the top and the bottom of the elastic shrinkage component are respectively fixedly connected with the top of the inner wall of the corresponding cylinder and the top of the T-shaped plate.
Optionally, the constant head tank has all been seted up to the positive left and right sides of arc, vertical rod, two are all installed to the left and right sides of T shaped plate upper surface the top of vertical rod runs through the arc respectively in proper order and with the inside looks joint that corresponds the constant head tank.
The invention provides a flow cytometry analyzer for in vitro detection, which has the following beneficial effects:
1. this flow cytometry appearance for external detection through the annular swing joint mode between arc pole and the arc pipe, and convenient to use person limits the position of collector, through the joint connected mode between constant head tank and the vertical rod, and convenient to use person fixes the position of collector, and the elastic potential energy person of facilitating the use of T shaped plate through the elastic shrinkage part changes the collector, has improved this convenience that flow cytometry changes the collector for external detection.
2. This flow cytometry appearance for external detection through the cooperation between constant head tank, vertical rod, arc and the cell sorter, and the person of being convenient for installs and fastens according to the model size of collector, has improved this flow cytometry appearance for external detection's suitability.
Drawings
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a schematic side view of the present invention;
FIG. 3 is a schematic top view of the arced tube of FIG. 1;
FIG. 4 is an enlarged schematic view at A of FIG. 1;
fig. 5 is a top view of the anti-slip device of fig. 3.
In the figure: 1. a base; 2. a body; 3. a collector; 4. a control panel; 5. an anti-skid device; 51. a transverse plate; 52. a transverse slot; 6. a fixing plate; 7. a T-shaped plate; 8. a limiting rod; 9. a barrel; 10. a limiting plate; 11. fixing a column; 12. an elastic contracting member; 13. positioning a groove; 14. a vertical rod; 15. an arc-shaped plate; 16. a cell sorter; 17. an arc-shaped clamping and connecting plate; 18. an arcuate bar; 19. an arc-shaped tube.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, a flow cytometer for in vitro detection includes a base 1, a body 2 fixedly connected to the left side of the upper surface of the base 1, a cell sorter 16 fixedly connected to the middle portion of the right side of the lower surface of the body 2, a charging circuit installed inside the base 1, a charging circuit on the base 1 charges selected cell droplets, the charged droplets carry cells through an electrostatic field and then deflect, and then fall into a collector, other liquid is sucked as waste liquid, a limiting rod 8 is fixedly connected to the lower surface of the body 2, the number of the limiting rods 8 is two, the two limiting rods 8 are respectively located at two sides of the cell sorter 16 and are mutually symmetrical with the center line of the cell sorter 16 in the vertical direction as a symmetry axis, so that a user can conveniently install and fasten the flow cytometer for in vitro detection according to the size of the type of the collector 3, thereby improving the applicability of the flow cytometer for in vitro detection, the bottom of the cell sorter 16 is provided with a collector 3, the right side of the collector 3 is fixedly connected with a control plate 4, and the outer surface of the collector 3 is provided with an arc-shaped pipe 19.
Wherein, the right side fixedly connected with fixed plate 6 of base 1 upper surface, fixed plate 6 is located collector 3 under, and the interval between the bottom of the top distance collector 3 of fixed plate 6 is three centimetres.
Wherein, the quantity of arc pipe 19 is four, through arc 18 swing joint between four arc pipes 19 two double-phase neighbours, through the annular swing joint mode between arc 18 and arc 19, convenient to use person limits the position of collector 3, the equal fixedly connected with arc joint board 17 in one side in four arc pipes 19 looks, two equal fixedly connected with antiskid 5 in the outside that are located the arc 19 of the collector 3 left and right sides respectively, two equal fixedly connected with arcs 15 in the outside that are located the arc 19 of the collector 3 upper and lower both sides respectively.
The bottom of the arc-shaped clamping plate 17 is clamped with the back of the outer edge of the collector 3, the arc-shaped clamping plate 17 is in an isosceles trapezoid shape, and the length of a connecting line between every two adjacent arc-shaped clamping plates 17 is equal.
The anti-skid device 5 comprises a transverse plate 51, a transverse groove 52 is formed in the front face of the transverse plate 51, and the inner portion of the transverse groove 52 is movably connected with the outer surface of the limiting rod 8.
Wherein, the below of arc 15 is equipped with T shaped plate 7, and the top of T shaped plate 7 runs through arc 15 and barrel 9 in proper order and extends to the inside of barrel 9, and the surface of T shaped plate 7 respectively with the middle part of arc 15 and the bottom swing joint of barrel 9.
Wherein, the bottom fixedly connected with barrel 9 of organism 2, limiting plate 10 has been cup jointed in the inside activity of barrel 9, the outside swing joint of limiting plate 10's inside and T shaped plate 7, the inside fixedly connected with fixed column 11 of barrel 9.
Wherein, the bottom of fixed column 11 and the top activity of T shaped plate 7 cup joint, and the surface activity of fixed column 11 has cup jointed elastic contraction part 12, and the elastic potential energy facilitate the user to T shaped plate 7 through elastic contraction part 12 changes collector 3, has improved this external detection and has changed the convenience of collector 3 with flow cytometry appearance, the top and the bottom of elastic contraction part 12 respectively with the top that corresponds barrel 9 inner wall and the top fixed connection of T shaped plate 7.
Wherein, constant head tank 13 has all been seted up to the positive left and right sides of arc 15, and vertical rod 14 is all installed to the left and right sides of T shaped plate 7 upper surface, and through the joint connected mode between constant head tank 13 and the vertical rod 14, convenient to use person fixes the position of collector 3, and the top of two vertical rods 14 runs through arc 15 respectively in proper order and with the interior looks joint that corresponds constant head tank 13.
When the cell sorter works, firstly, a worker places cells to be detected in the collector 3, then the top of the collector 3 is fixed on a detector of the base 1 through the arc-shaped clamping plates 17 by holding the control plate 4 fixedly connected with the right side of the collector 3, in the fixing process, the worker needs to pull the T-shaped plate 7 and separate the top of the fixed column 11 from the inside of the positioning groove 13, then push the arc-shaped clamping plates 17 on the left side and the right side to move towards one side close to the collector 3, the two arc-shaped clamping plates 17 are always kept in the same horizontal plane in the pushing process, meanwhile, the worker loosens the T-shaped plate 7 and drives the vertical rod 14 to move upwards through the elastic restoring force of the elastic contraction component 12, then the top end of the vertical rod 14 is clamped with the inside of the corresponding positioning groove 13, and when the user needs to sort, the sprayed liquid column is divided into a series of small water drops through the nozzle on the cell sorter 16, whether or not it is to be sorted is determined by the logic circuit based on the selected correspondence.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. A flow cytometry analyzer for in vitro detection comprises a base (1), and is characterized in that: the cell sorter is characterized in that an organism (2) is fixedly connected to the left side of the upper surface of a base (1), a cell sorter (16) is fixedly connected to the middle portion of the right side of the lower surface of the organism (2), limiting rods (8) are fixedly connected to the lower surface of the organism (2), the two limiting rods (8) are respectively located on two sides of the cell sorter (16) and are mutually symmetrical by taking the central line of the vertical direction of the cell sorter (16) as a symmetrical axis, a collector (3) is arranged at the bottom of the cell sorter (16), a control plate (4) is fixedly connected to the right side of the collector (3), arc tubes (19) are arranged on the outer surface of the collector (3), the number of the arc tubes (19) is four, the four arc tubes (19) are movably connected between every two adjacent arc rods (18), and arc clamping plates (17) are fixedly connected to one opposite sides of the four arc tubes (19), the bottom of the arc-shaped clamping plate (17) is clamped with the back of the outer edge of the collector (3), the arc-shaped clamping plate (17) is isosceles trapezoid, the length of a connecting line between every two adjacent arc-shaped clamping plates (17) is equal, the two connecting lines are respectively positioned on the left side and the right side of the collector (3), an anti-slip device (5) is fixedly connected to the outer side of the arc-shaped pipe (19), the anti-slip device (5) comprises a transverse plate (51), a transverse groove (52) is formed in the front of the transverse plate (51), the inner part of the transverse groove (52) is movably connected with the outer surface of a limiting rod (8), the two outer sides of the arc-shaped pipe (19) which are respectively positioned on the upper side and the lower side of the collector (3) are fixedly connected with arc-shaped plates (15), a T-shaped plate (7) is arranged below the arc-shaped plates (15), the top end of the T-shaped plate (7) sequentially penetrates through the arc-shaped plates (15) and the barrel (9) and extends to the inner part of the barrel (9), the surface of T shaped plate (7) respectively with the middle part of arc (15) and the bottom swing joint of barrel (9), the bottom fixedly connected with barrel (9) of organism (2), limiting plate (10) have been cup jointed in the inside activity of barrel (9), the inside of limiting plate (10) and the surface swing joint of T shaped plate (7), the inside fixedly connected with fixed column (11) of barrel (9), the bottom of fixed column (11) and the top activity of T shaped plate (7) cup joint, the surface activity of fixed column (11) cup joints elastic shrinkage part (12), the top and the bottom of elastic shrinkage part (12) respectively with the top that corresponds barrel (9) inner wall and the top fixed connection of T shaped plate (7).
2. A flow cytometer for in vitro detection according to claim 1, wherein: the right side fixedly connected with fixed plate (6) of base (1) upper surface, fixed plate (6) are located collector (3) under, the interval between the top of fixed plate (6) and the bottom of collector (3) is three centimetres.
3. A flow cytometer for in vitro detection according to claim 1, wherein: constant head tank (13) have all been seted up to the positive left and right sides of arc (15), vertical rod (14), two are all installed to the left and right sides of T shaped plate (7) upper surface the top of vertical rod (14) runs through arc (15) respectively in proper order and with the inside looks joint that corresponds constant head tank (13).
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| CN201910562735.6A CN110220836B (en) | 2019-06-26 | 2019-06-26 | Flow cytometry analyzer for in-vitro detection |
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