CN110218649A - A kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure - Google Patents
A kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure Download PDFInfo
- Publication number
- CN110218649A CN110218649A CN201910587623.6A CN201910587623A CN110218649A CN 110218649 A CN110218649 A CN 110218649A CN 201910587623 A CN201910587623 A CN 201910587623A CN 110218649 A CN110218649 A CN 110218649A
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- culture vessel
- cell culture
- attached cell
- main body
- multilayer
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- 238000004113 cell culture Methods 0.000 title claims abstract description 66
- 230000011218 segmentation Effects 0.000 claims abstract description 26
- 239000012528 membrane Substances 0.000 claims abstract description 23
- 210000004027 cell Anatomy 0.000 claims description 12
- 230000002209 hydrophobic effect Effects 0.000 claims description 5
- 238000000748 compression moulding Methods 0.000 claims description 4
- 238000009826 distribution Methods 0.000 claims description 4
- 210000001711 oxyntic cell Anatomy 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 abstract description 4
- 239000010410 layer Substances 0.000 description 22
- 230000001464 adherent effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005253 cladding Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to culture vessel fields, in particular a kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure, including multilayer attached cell culture vessel main body, multi-layer segmentation plate and lid with lug, the multilayer attached cell culture vessel main body upper end side is equipped with installing port at basal plane and the back side, the installing port upper end is fixedly installed with lid with lug, rectangular bayonet is offered at the lid with lug upper end center, the multilayer attached cell culture vessel body interior is equidistantly equipped with multiple groups multi-layer segmentation plate from top to bottom, the multi-layer segmentation plate is equipped with cell at installing port and enters through-hole, the multilayer attached cell culture vessel main body is equipped with locating groove at multi-layer segmentation plate, ventilated membrane is equipped at the multilayer attached cell culture vessel main body basal plane and the back side.The application of ventilated membrane makes the container can be used for expiring liquid culture in the present invention, reduces layer height, with culture area is considerably increased under height, improves space utilization rate.
Description
Technical field
The present invention relates to culture vessel field, specially a kind of novel two sides gas permeable multiple shelf attached cell culture vessel knot
Structure.
Background technique
Anchorage-dependent cells will be attached on culture vessel wall in culture, and cell is just sprawled rapidly once adherent, so
After start mitosis, and enter logarithmic growth phase quickly, with regard to confluent cultures surface after general a couple of days, and form fine and close cell
Single layer.
The shortcomings that adhere-wall culture: expansion culture is relatively difficult compared with Maitland culture, and investment is big, takes up a large area, more
Layer attached cell culture vessel increases with the number of plies, and interlayer will have certain altitude to meet ventilative demand, if there is full liquid culture
Mode equally reaches certain Air permenbility, then can greatly shorten interfloor height, saves space, increases culture area, need to make
Gas permeability inside container meets the needs of cell, it is therefore desirable to a kind of novel two sides gas permeable multiple shelf attached cell culture vessel knot
Structure makes improvement to the above problem.
Summary of the invention
The purpose of the present invention is to provide a kind of novel two sides gas permeable multiple shelf attached cell culture vessel structures, on solving
State the problem of proposing in background technique.
To achieve the above object, the invention provides the following technical scheme:
A kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure, including multilayer attached cell culture vessel master
Body, multi-layer segmentation plate and lid with lug, the multilayer attached cell culture vessel main body upper end side is at basal plane and the back side
It is equipped with installing port, the installing port upper end is fixedly installed with lid with lug, offers square card at the lid with lug upper end center
Mouthful, the multilayer attached cell culture vessel body interior is equidistantly equipped with multiple groups multi-layer segmentation plate, the multilayer from top to bottom
Reticle is equipped with cell at installing port and enters through-hole, and the multilayer attached cell culture vessel main body is close to multi-layer segmentation
It is equipped with locating groove at plate, is equipped with ventilated membrane at the multilayer attached cell culture vessel main body basal plane and the back side, it is described more
Culture solution is equipped in layer attached cell culture vessel main body.
Preferably, the installing port and multilayer attached cell culture vessel main body are an integral structure, the lid with lug and
Multilayer attached cell culture vessel main body is socketed by installing port and is connected.
Preferably, the lid with lug and external automatically opening and closing machinery pass through the clamping connection of rectangular bayonet.
Preferably, the ventilated membrane selects 0.2 μm of hydrophobic air permeable film, and the ventilated membrane and the culture of multilayer attached cell
Compression moulding between container body.
Preferably, the ventilated membrane and multi-layer segmentation plate are in corresponding distribution.
Preferably, it is clamped and is connected by locating groove between the multi-layer segmentation plate and multilayer attached cell culture vessel main body
It connects.
Compared with prior art, the beneficial effects of the present invention are:
1, in the present invention, two sides cladding has the ventilative of certain airing function in multilayer attached cell culture vessel main body
Film makes every layer of culture solution that can have certain uniform Air permenbility, to meet the culture demand of attached cell, ventilated membrane
Application so that the container is can be used for expiring liquid culture, it is high to reduce layer, with culture area is considerably increased under height, improves space benefit
With rate.
2, in the present invention, lid with lug provides the structure of automated machine operation, and what rectangular bayonet offer was screwed/unscrewed determines
, limit provides the positioning of bottle cap or more folding inside lid with lug, and the application of lid with lug makes multilayer attached cell culture vessel master
Body has the condition of automated production, and the application of lid with lug makes multilayer attached cell culture vessel main body be suitable for automated machine
Production.
3, in the present invention, installing port and multilayer attached cell culture vessel main body are an integral structure, lid with lug and multilayer
Attached cell culture vessel main body is socketed by installing port and is connected, and lid with lug and external automatically opening and closing machinery pass through rectangular bayonet
Clamping connection, ventilated membrane selects 0.2 μm of hydrophobic air permeable film, and presses between ventilated membrane and multilayer attached cell culture vessel main body
Type, ventilated membrane and multi-layer segmentation plate is made in corresponding distribution, between multi-layer segmentation plate and multilayer attached cell culture vessel main body
It is clamped and is connected by locating groove, whole structure assembling is simple, it is easy to accomplish.
Detailed description of the invention
Fig. 1 is a kind of whole three view of novel two sides gas permeable multiple shelf attached cell culture vessel structure proposed by the present invention
Figure；
Fig. 2 is a kind of lid with lug structure of novel two sides gas permeable multiple shelf attached cell culture vessel structure proposed by the present invention
Schematic diagram；
Fig. 3 is that a kind of perspective structure of novel two sides gas permeable multiple shelf attached cell culture vessel structure proposed by the present invention shows
It is intended to；
Fig. 4 is that a kind of multilayer of novel two sides gas permeable multiple shelf attached cell culture vessel structure proposed by the present invention is adherent thin
Born of the same parents' culture vessel agent structure schematic diagram.
In figure: 1- multilayer attached cell culture vessel main body, 2- multi-layer segmentation plate, 3- lid with lug, the rectangular bayonet of 4-, 5- are thin
Born of the same parents enter through-hole, 6- culture solution, 7- installing port, 8- locating groove, 9- ventilated membrane.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1-4 is please referred to, the present invention provides a kind of technical solution:
A kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure, including multilayer attached cell culture vessel main body
1, multi-layer segmentation plate 2 and lid with lug 3,1 upper end side of multilayer attached cell culture vessel main body are equal at basal plane and the back side
Equipped with installing port 7,7 upper end of installing port is fixedly installed with lid with lug 3, and installing port 7 and multilayer attached cell culture vessel main body 1 are
Integral structure, lid with lug 3 and multilayer attached cell culture vessel main body 1 pass through the socket connection of installing port 7,3 upper end of lid with lug
Rectangular bayonet 4, lid with lug 3 and external automatically opening and closing machinery are offered at center passes through the clamping connection of rectangular bayonet 4, multilayer patch
Multiple groups multi-layer segmentation plate 2 is equidistantly equipped with inside parietal cell culture vessel main body 1 from top to bottom, multi-layer segmentation plate 2 is close to installation
It is equipped with cell at mouth 7 and enters through-hole 5, multilayer attached cell culture vessel main body 1 is equipped with positioning card at multi-layer segmentation plate 2
Slot 8, by the clamping connection of locating groove 8 between multi-layer segmentation plate 2 and multilayer attached cell culture vessel main body 1, multilayer is adherent
Ventilated membrane 9 is equipped at 1 basal plane of cell culture container main body and the back side, ventilated membrane 9 selects 0.2 μm of hydrophobic air permeable film, and thoroughly
Compression moulding between air film 9 and multilayer attached cell culture vessel main body 1, ventilated membrane 9 and multi-layer segmentation plate 2 are distributed in corresponding,
Culture solution 6 is equipped in multilayer attached cell culture vessel main body 1.
Workflow of the present invention: it is socketed by lid with lug 3 and multilayer attached cell culture vessel main body 1 by installing port 7
Connection, installing port 7 and multilayer attached cell culture vessel main body 1 are an integral structure, lid with lug 3 and external automatically opening and closing machine
Tool selects 0.2 μm of hydrophobic air permeable film, and ventilated membrane 9 and multilayer attached cell by the clamping connection of rectangular bayonet 4, ventilated membrane 9
Compression moulding between culture vessel main body 1, ventilated membrane 9 and multi-layer segmentation plate 2 are in corresponding distribution, multi-layer segmentation plate 2 and multilayer patch
By the clamping connection of locating groove 8 between parietal cell culture vessel main body 1, whole structure assembling is simple, it is easy to accomplish, multilayer
Two sides cladding has the ventilated membrane 9 of certain airing function in attached cell culture vessel main body 1, and every layer of culture solution 6 is made all may be used
To there is certain uniform Air permenbility, to meet the culture demand of attached cell, the application of ventilated membrane 9 can be used for the container
Full liquid culture reduces layer height, with culture area is considerably increased under height, improves space utilization rate, lid with lug 3 provides automatically
Change mechanically operated structure, rectangular bayonet 4 provides the positioning for screwing/unscrewing, and limit provides bottle cap and opens and closes up and down inside lid with lug 3
Positioning, the application of lid with lug 3 makes multilayer attached cell culture vessel main body 1 have the condition of automated production, lid with lug 3
It is produced using making multilayer attached cell culture vessel main body 1 be suitable for automated machine.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure, including multilayer attached cell culture vessel main body
(1), multi-layer segmentation plate (2) and lid with lug (3), it is characterised in that: multilayer attached cell culture vessel main body (1) upper end
Side is equipped with installing port (7) at basal plane and the back side, and installing port (7) upper end is fixedly installed with lid with lug (3), described
It is offered rectangular bayonet (4) at lid with lug (3) upper end center, the multilayer attached cell culture vessel main body (1) is internal from upper
Equidistantly be equipped with multiple groups multi-layer segmentation plate (2) to lower, the multi-layer segmentation plate (2) be equipped at installing port (7) cell into
Entering through-hole (5), the multilayer attached cell culture vessel main body (1) is equipped with locating groove (8) at multi-layer segmentation plate (2),
It is equipped with ventilated membrane (9) at multilayer attached cell culture vessel main body (1) basal plane and the back side, the multilayer attached cell training
It supports in container body (1) and is equipped with culture solution (6).
2. a kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure according to claim 1, it is characterised in that:
The installing port (7) and multilayer attached cell culture vessel main body (1) are an integral structure, the lid with lug (3) and multilayer patch
Parietal cell culture vessel main body (1) passes through installing port (7) socket connection.
3. a kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure according to claim 1, it is characterised in that:
The lid with lug (3) and external automatically opening and closing machinery pass through rectangular bayonet (4) clamping connection.
4. a kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure according to claim 1, it is characterised in that:
The ventilated membrane (9) selects 0.2 μm of hydrophobic air permeable film, and the ventilated membrane (9) and multilayer attached cell culture vessel main body
(1) compression moulding between.
5. a kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure according to claim 1, it is characterised in that:
The ventilated membrane (9) and multi-layer segmentation plate (2) are in corresponding distribution.
6. a kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure according to claim 1, it is characterised in that:
Pass through locating groove (8) clamping connection between the multi-layer segmentation plate (2) and multilayer attached cell culture vessel main body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910587623.6A CN110218649A (en) | 2019-07-02 | 2019-07-02 | A kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910587623.6A CN110218649A (en) | 2019-07-02 | 2019-07-02 | A kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110218649A true CN110218649A (en) | 2019-09-10 |
Family
ID=67815823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910587623.6A Pending CN110218649A (en) | 2019-07-02 | 2019-07-02 | A kind of novel two sides gas permeable multiple shelf attached cell culture vessel structure |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101120083A (en) * | 2003-10-08 | 2008-02-06 | 威尔森沃尔夫制造公司 | Method and device for cell culture using gas permeable material |
| CN101611132A (en) * | 2006-12-07 | 2009-12-23 | 威尔森沃尔夫制造公司 | Efficient ventilation device and method for culturing cells |
| EP2821145A1 (en) * | 2013-07-02 | 2015-01-07 | Miltenyi Biotec GmbH | Centrifugation chamber with gas-permeable membranes layers for cell cultivation |
| CN107312712A (en) * | 2017-07-13 | 2017-11-03 | 广州洁特生物过滤股份有限公司 | A kind of multi-layer cellular culture apparatus |
| CN109207364A (en) * | 2018-10-11 | 2019-01-15 | 广州洁特生物过滤股份有限公司 | A kind of cell culture apparatus |
-
2019
- 2019-07-02 CN CN201910587623.6A patent/CN110218649A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101120083A (en) * | 2003-10-08 | 2008-02-06 | 威尔森沃尔夫制造公司 | Method and device for cell culture using gas permeable material |
| CN101611132A (en) * | 2006-12-07 | 2009-12-23 | 威尔森沃尔夫制造公司 | Efficient ventilation device and method for culturing cells |
| EP2821145A1 (en) * | 2013-07-02 | 2015-01-07 | Miltenyi Biotec GmbH | Centrifugation chamber with gas-permeable membranes layers for cell cultivation |
| CN107312712A (en) * | 2017-07-13 | 2017-11-03 | 广州洁特生物过滤股份有限公司 | A kind of multi-layer cellular culture apparatus |
| CN109207364A (en) * | 2018-10-11 | 2019-01-15 | 广州洁特生物过滤股份有限公司 | A kind of cell culture apparatus |
Non-Patent Citations (2)
| Title |
|---|
| FRANK BUNGE ET AL.: "Gas Supply through Agarose Walls in Cell Culturing Microchips", 《ADVANCES IN SCIENCE AND TECHNOLOGY》 * |
| 田澍等: "多层细胞工厂在水痘-带状疱疹减毒毒种(OKa株)传代中的应用", 《中国生物制品学杂志》 * |
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Application publication date: 20190910 |