CN110214775A - A kind of self hair follicle preservation liquid - Google Patents
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- CN110214775A CN110214775A CN201910256035.4A CN201910256035A CN110214775A CN 110214775 A CN110214775 A CN 110214775A CN 201910256035 A CN201910256035 A CN 201910256035A CN 110214775 A CN110214775 A CN 110214775A
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- 238000005119 centrifugation Methods 0.000 claims abstract 4
- 238000002360 preparation method Methods 0.000 claims description 4
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 2
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- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/128—Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gels; Chemically altering living parts, e.g. by cross-linking
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
我国脱发的患者呈逐年增长的趋势，需要毛发移植的患者也是越来越多。目前现有的毛发移植术都是以毛囊为移植单位，又称自体毛囊移植术。由于毛囊单位及其微小，在提取和保存毛囊的时候，极易存在着毛囊细胞受损存活率不高的现象。本专发明了一种自体毛囊保存液，用于保存需要移植的毛囊，保存于自体毛囊保存液中的毛囊可以提高毛囊细胞的在体外的存活率，同时也保障了毛囊移植的成功率。自体毛囊保存液来源于自体外周血，通过在无菌实验室经过两步离心法得到的一种富含多种细胞因子的自体血浆。The number of patients with hair loss in my country is increasing year by year, and more and more patients need hair transplantation. At present, the existing hair transplantation is based on the hair follicle as the transplantation unit, also known as autologous hair follicle transplantation. Due to the extremely small size of the hair follicle unit, when extracting and storing hair follicles, it is very easy to have a phenomenon that the survival rate of hair follicle cells is not high due to damage. This patent has invented an autologous hair follicle preservation solution, which is used to preserve hair follicles that need to be transplanted. The hair follicles preserved in the autologous hair follicle preservation solution can improve the survival rate of hair follicle cells in vitro, and also ensure the success rate of hair follicle transplantation. Autologous hair follicle preservation solution is derived from autologous peripheral blood, which is a kind of autologous plasma rich in various cytokines obtained through two-step centrifugation in a sterile laboratory.
Description
技术领域technical field
本专利属于医疗美容行业。This patent belongs to the medical beauty industry.
背景技术Background technique
随着社会生产力的发展，现代社会进入一个快节奏时代，都市快节奏的生活伴随着高压、上网熬夜、加班、不良饮食、焦虑、失眠、头发烫染等负面行为的增多，而这些负面行为都是导致脱发的重要因素。近年来，我国已有一亿多脱发患者，发病呈逐年上升趋势，同时也出现低龄化。脱发现象严重性和紧迫性在一定程度上促进了毛发移植术的发展。目前现有的毛发移植术都是以毛囊为移植单位，又称自体毛囊移植术。由于毛囊单位及其微小，在提取和保存毛囊的时候，极易存在着毛囊细胞受损存活率不高的现象。特别是在大面积移植的时候，提取和保存毛囊时间较长，需要一种能够短期维持毛囊细胞活性的保存液，毛囊保存液的好坏决定了毛囊移植的成活率。本专利就是在临床实践在发现的一种能够更好保存毛囊并提高毛囊移植存活率的毛囊保存液。With the development of social productivity, modern society has entered a fast-paced era. The fast-paced life of the city is accompanied by the increase of negative behaviors such as high pressure, staying up late on the Internet, overtime, poor diet, anxiety, insomnia, hair dyeing, etc. These negative behaviors All are important factors leading to hair loss. In recent years, there have been more than 100 million alopecia patients in my country, and the incidence is increasing year by year, and the age is also younger. The severity and urgency of hair loss has promoted the development of hair transplantation to a certain extent. At present, the existing hair transplantation is based on the hair follicle as the transplantation unit, also known as autologous hair follicle transplantation. Due to the extremely small size of the hair follicle unit, when extracting and storing hair follicles, it is very easy to have a phenomenon that the survival rate of hair follicle cells is not high due to damage. Especially in the case of large-scale transplantation, it takes a long time to extract and preserve hair follicles, and a preservation solution that can maintain the activity of hair follicle cells in the short term is needed. The quality of the hair follicle preservation solution determines the survival rate of hair follicle transplantation. This patent is a hair follicle preservation solution discovered in clinical practice that can better preserve hair follicles and improve the survival rate of hair follicle transplantation.
发明内容Contents of the invention
一种自体毛囊保存液的制备原料来源于患者自身的外周血，没有加入任何外源性成分，完全来源于自体，保证了本发明的安全性。The raw material for the preparation of an autologous hair follicle preservation solution is derived from the patient's own peripheral blood without adding any exogenous components, and is completely autologous, which ensures the safety of the present invention.
一种自体毛囊保存液的制备过程包括：A preparation process of autologous hair follicle preservation solution comprises:
1.用带枸橼酸钠或者肝素抗凝剂的采血管采集外周血20-25ml，混匀；1. Use a blood collection tube with sodium citrate or heparin anticoagulant to collect 20-25ml of peripheral blood and mix well;
2.将采集的外周血全部转移到干净无菌的50ml离心管中，400g离心8min；2. Transfer all the collected peripheral blood to a clean and sterile 50ml centrifuge tube, and centrifuge at 400g for 8min;
3.将上层全部血浆吸取到另外一个干净无菌的50ml离心管中，1200g离心10min；3. Pipette all the upper plasma into another clean and sterile 50ml centrifuge tube, centrifuge at 1200g for 10min;
4.缓慢吸取血浆上层3/4废弃，离心管底部1/4的血浆即为自体毛囊保存液，一般有2-4ml左右，密封储存于4℃备用。4. Slowly absorb the upper 3/4 of the plasma and discard it. The bottom 1/4 of the plasma in the centrifuge tube is the autologous hair follicle preservation solution. Generally, there are about 2-4ml, and it is sealed and stored at 4°C for later use.
一种字体毛囊保存液在毛囊移植完成后还可以均匀涂抹于移植部位，有利于提高移植毛囊的成活。A font hair follicle preservation solution can be evenly applied to the transplanted site after the hair follicle transplantation is completed, which is beneficial to improve the survival of the transplanted hair follicles.
一种自体毛囊保存液主要成分及功能如下：血小板：促进凝血，释放多种功能性细胞因子；转化生长因子β（TGF-β）：促进未分化的间质细胞增殖分化，调控内皮细胞、成纤维细胞有丝分裂，促进胶原纤维合成及胶原蛋白酶的释放；血小板源性生长因子（PDGF）：促进间质细胞的有丝分裂促进巨噬细胞和中性粒细胞的趋化；成纤维细胞生长因子（FGF）：促进成纤维细胞和软骨细胞的有丝分裂和分化；表皮细胞生长因子（EGF）：促进血管内皮细胞增生，调节胶原蛋白酶分泌，促进上皮细胞及间质细胞有丝分裂；血管内皮生长因子（VEGF）：促进血管生成，提高血管通透性，促进血管内皮细胞有丝分裂。The main components and functions of an autologous hair follicle preservation solution are as follows: platelets: promote blood coagulation, release a variety of functional cytokines; transforming growth factor β (TGF-β): promote the proliferation and differentiation of undifferentiated mesenchymal cells, regulate endothelial cells, Fibroblast mitosis, promoting collagen fiber synthesis and release of collagenase; platelet-derived growth factor (PDGF): promoting mitosis of mesenchymal cells and promoting chemotaxis of macrophages and neutrophils; fibroblast growth factor (FGF) : promote the mitosis and differentiation of fibroblasts and chondrocytes; epidermal growth factor (EGF): promote the proliferation of vascular endothelial cells, regulate the secretion of collagenase, and promote the mitosis of epithelial cells and mesenchymal cells; vascular endothelial growth factor (VEGF): promote Angiogenesis, increase vascular permeability, and promote mitosis of vascular endothelial cells.
Claims (4)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114946829A (en) * | 2022-04-06 | 2022-08-30 | 浙江卫未生物医药科技有限公司 | Follicle tissue vitrification freezing solution |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114946829A (en) * | 2022-04-06 | 2022-08-30 | 浙江卫未生物医药科技有限公司 | Follicle tissue vitrification freezing solution |
| CN114946829B (en) * | 2022-04-06 | 2023-11-03 | 浙江卫未生物医药科技有限公司 | A kind of hair follicle tissue vitrification cryopreservation solution |
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