CN110199994A - A kind of disinfectant and preparation method thereof, application, feed addictive - Google Patents

Abstract

The invention relates to a disinfectant, its preparation method and application, and a feed additive, including active ingredients, an acidulant and water; the active ingredients include: 0.001%-0.06% of thymol, lauric acid in terms of mass percentage of the disinfectant 0.01%-0.1% monoglyceride, 0.5-5% dehydrated alcohol; wherein, the acidulant is selected from: citric acid 0.01-0.4%, calcium formate 1%-1.5% in terms of mass percentage of the disinfectant , at least one of calcium propionate 1% to 1.5%. The disinfectant formula of the present invention is relatively simple, green and environmentally friendly, non-toxic, less irritating, and has few side effects. By forming a stable microemulsion system with hydrophobic thymol, monolauric acid monoglyceride, absolute ethanol, etc., the sterilization rate and sterilization The virus rate can reach more than 99.99%. It is a non-toxic, efficient, quick-acting, broad-spectrum, stable and safe compound disinfectant formula.

Description

A kind of disinfectant and its preparation method, application, feed additive

technical field

The invention relates to the field of disinfectant preparation, in particular to a disinfectant, its preparation method and application, and a feed additive.

Background technique

Disinfection work is the key point that all walks of life need to pay attention to, because only by doing a good job in disinfection can we achieve the purpose of preventing diseases and maintaining hygiene, and then achieve the purpose of cutting the transmission route of pathogens and preventing and controlling the occurrence of infection.

Disinfection in many fields, especially the field of livestock and poultry, is particularly important. Since the outbreak of African swine fever in China in 2018, the development of the pig industry has been severely tested. In the livestock and poultry breeding industry, the transmission route of many pathogenic bacteria is mainly through contact with air and pathogens, so the disinfection of all aspects of breeding is an important part of biosafety prevention and control.

Caustic soda, among the mainstream disinfectants currently on the market, is severely corrosive and irritating, while formaldehyde, bleaching powder, and sodium carbonate are all corrosive and irritating to a certain extent, and there is a danger of secondary pollution.

In view of this, the present invention is proposed.

Contents of the invention

The first object of the present invention is to provide a disinfectant formula, which is relatively simple, environmentally friendly, non-toxic, less irritating, and has less side effects. After compounding, a stable microemulsion system is formed. The addition of acidifier can further improve the stability and bactericidal effect of the disinfectant. The bactericidal rate and virus killing rate can reach more than 99.99%. It is non-toxic, efficient, quick-acting, broad-spectrum, stable, Safe compound disinfectant formula.

The second object of the present invention is to provide a preparation method of the above-mentioned disinfectant, which has the advantages of being able to completely retain the active ingredients of each component, and has the advantages of simple method, easy operation, and mild operating conditions.

The third object of the present invention is to provide the application of the above-mentioned disinfectant, which can be widely used in disinfection in various fields, especially in the field of livestock and poultry disinfection, which is therefore worthy of wide application.

In order to realize the above-mentioned purpose of the present invention, special adopt following technical scheme:

The invention provides a disinfectant comprising an active ingredient, an acidulant and water;

The active ingredients include: 0.001% to 0.06% of thymol, 0.01% to 0.1% of monoglyceride laurate, and 0.5% to 5% of absolute ethanol in terms of mass percentage of the disinfectant;

Wherein, the acidifying agent is selected from at least one of: 0.01-0.4% citric acid, 1%-1.5% calcium formate, and 1%-1.5% calcium propionate, based on the mass percentage of the disinfectant.

Among them, thymol belongs to thyme essential oil extract, which is a hydrophobic substance with the characteristics of green, safe and efficient, and is a natural food additive and pharmaceutical ingredient approved by FDA, which can partially or completely replace traditional chemical preservatives.

In addition, thymol is also a natural antioxidant, which has a strong ability to scavenge free radicals. However, its water solubility problem has always limited its wide use, not only the high cost of use, but also the undissolved part will affect the biological activity of the product. Since the vast majority of microorganisms can only grow and reproduce in the water phase, and all enzymatic biochemical reactions and antioxidant reactions related to life activities are carried out in the water phase, only thymol is fully dissolved or evenly dispersed in the water phase , in order to act on the target and exert its biological efficacy.

Monolauric acid monoglyceride is not only an excellent emulsifier, but also a safe, efficient and broad-spectrum antibacterial agent, which can inhibit the replication and spread of enveloped viruses (AIDS, PRRS, swine fever, African swine fever virus). Being insoluble in water.

Anhydrous ethanol can be made into 70%-75% ethanol as a disinfectant, but it is highly irritating, flammable and volatile. Ethanol can act as a cosurfactant to promote the formation of microemulsions by reducing the interfacial tension between oil and water, improving the spontaneous curvature of the interface, and reducing the stiffness of the interface.

Citric acid dissolves in water to produce H ⁺ and free radicals, and the H ⁺ produced can change the pH value of the cells, thereby changing the living environment of microorganisms. It is widely used in seasoning and antisepsis in the food industry, disinfection of precision medical equipment, and environmental protection and antifouling and other fields, but its acidity is too strong, which is strongly irritating to the gastrointestinal tract.

As a feed additive, calcium formate and calcium propionate will release a small amount of formic acid in the animal body, which will lower the pH value of the gastrointestinal tract, and have a buffering effect to stabilize the pH value of the gastrointestinal tract, thereby inhibiting the reproduction of harmful bacteria and promoting beneficial bacteria. microbial growth.

In the solution of the present invention, the acidulant has only selected any one or more of these three kinds of citric acid, calcium formate, and calcium propionate, because not all acidulants have the effect of aiding dissolution and disinfection, so It is necessary to choose purposefully.

In order to overcome the severe corrosiveness and irritation of environmental disinfectants in the existing market, and the defects of non-biodegradation and secondary hazards, the present invention fills the blank that cannot be taken orally, soaked, and sprayed on the surface of livestock and poultry for disinfection. After screening a large number of formulations, all edible components such as thymol, monolaurate, organic acid and ethanol were selected to construct a stable microemulsion system.

In the present invention, the above unilateral prescriptions are compounded, and microemulsification technology is used to solve the water solubility of thymol, and the ratio of surfactant, oil phase and co-surfactant is selected to determine the best multi-component system. Through the evaluation of the disinfection effect of the system, it is found that the disinfection effect of the compound formula is better than that of the single formula and greatly reduces the cost of disinfection. It can be safely and effectively used for disinfection of livestock and poultry environments and drinking water. Moreover, the disinfectant is non-irritating, green and environmentally friendly, and has excellent sterilization and disinfection effects .

Also, the surfactant monolaurate was chosen because applicants found that anionic and cationic surfactants were difficult to form microemulsions at high dilutions and non-ionic surfactants facilitated the formation of high concentration The formation of surfactant microemulsion system, but the commonly used non-ionic Tween is non-toxic to bacteria, and Tween 80 can even stimulate the growth of Mycobacterium tuberculosis. Inhibitory properties of viruses and Gram-positive bacteria.

In terms of dosage, the disinfection effect of the disinfectant can be significantly improved by adjusting it within a suitable and reasonable ratio range, and the concentration of ethanol in the system is reduced to between 0.5% and 5%, greatly reducing its potential safety hazard.

Preferably, as a further feasible solution, the acidulant is selected from the group consisting of: 0.01-0.4% citric acid, 1%-1.5% calcium formate and 1%-1.5% calcium propionate in terms of mass percentage of the disinfectant , the combination of the three has a better effect, so that while exerting its own antibacterial effect, the pH value of the system is reduced. Through research, it is found that when the pH drops below 5, the antibacterial ability of thymol can be greatly enhanced.

Preferably, as a further implementable solution, the acidulant is 0.01-0.4% citric acid. The cost of citric acid itself is relatively low, which can ensure that its disinfection effect is optimal under the condition of low cost.

Preferably, as a further practicable scheme, in terms of mass percentage, thymol is 0.006% to 0.03%, monoglyceride laurate is 0.02% to 0.06%, citric acid is 0.04% to 0.2%, and absolute ethanol is 1% to 4%. , and the balance is water.

Preferably, as a more optimal solution, in terms of mass percentage, thymol is 0.06%, lauric monoglyceride is 0.05%, citric acid is 0.4%, absolute ethanol is 0.5%, and the balance is water.

Preferably, a more optimal solution can also be proportioned according to the following formula, in terms of mass percentage, 0.06% thymol, 0.1% monoglyceride laurate, 0.4% citric acid, 0.5% absolute ethanol, and the balance is water.

Besides, the content of thymol can also be 0.002%, 0.005%, 0.01%, 0.02%, 0.04%, 0.05%, etc.

The content of monoglyceride laurate can also be 0.03%, 0.04%, 0.05%, 0.07%, 0.08%, 0.09%, etc.

The content of absolute ethanol can also be 1.5%, 2%, 2.5%, 3%, 3.5% and so on.

In addition to providing the formula of disinfectant, the present invention also provides the preparation method of above-mentioned disinfectant, comprises the steps:

(A) stirring and dissolving thymol and absolute ethanol to obtain the first mixed solution;

(B) stirring the solution with monoglyceride laurate and the remaining amount of absolute ethanol to obtain the second mixed solution;

(C) A third mixed liquid is formed after the acidulant is mixed and dissolved with a small amount of water;

Wherein steps (A), (B) and (C) have no sequence;

(D) Mix the first mixed liquid, the second mixed liquid and the third mixed liquid evenly, and add water to the final volume.

The preparation method of the invention is simple to operate, and the operation method is easy to implement, and the uniformity of the disinfectant after mixing can be improved by controlling the addition sequence.

Preferably, as a further feasible solution, in the step (D), the mixing is carried out by high-speed stirring in a homogenizer;

Preferably, the second mixed liquid is mixed with the first mixed liquid and then mixed with the third mixed liquid.

The invention provides a feed additive comprising the above-mentioned disinfectant and the disinfectant obtained by the above-mentioned preparation method.

Preferably, the feed additive also contains hawthorn, licorice, and lactose, and the addition of these substances can promote the appetite of livestock and poultry.

The disinfectant of the present invention can be widely used in livestock and poultry disinfection, drinking water disinfection, environmental disinfection, etc., as long as there is a need for disinfection.

Compared with prior art, the beneficial effect of the present invention is:

(1) The disinfectant of the present invention forms a stable microemulsion system with hydrophobic thymol, monoglyceride laurate, dehydrated alcohol, etc., and the bactericidal rate and virus killing rate can reach more than 99.99%;

(2) disinfectant of the present invention is nontoxic, efficient, quick-acting, broad-spectrum, stable, safe compound disinfectant formula;

(3) The disinfectant is widely used, especially in the field of disinfection of livestock and poultry, and has wide adaptability.

Detailed ways

Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention, and should not be considered as limiting the scope of the present invention. For those not indicated in the specific conditions in the examples, they were carried out according to the conventional conditions or the conditions suggested by the manufacturer, and the reagents or instruments used for those not indicated by the manufacturer were all conventional products that could be purchased commercially.

The solution of the present invention will be described below through specific embodiments:

Example 1

The formula (concentration by mass percentage) of disinfectant is to be made up of following components:

Thymol 0.03%;

Monoglyceride Laurate 0.05%;

Calcium formate 1.5%;

Absolute ethanol 5%;

water balance.

The preparation method of disinfectant is as follows:

1. Weigh each component according to the formula amount, first dissolve thymol in a small amount of co-solvent absolute ethanol in the first container, stir at high speed until completely dissolved;

2. Take the second container and weigh monoglyceride laurate, add absolute ethanol to help dissolve;

3. Take the third container and weigh calcium formate, add a small amount of water to dissolve, stir at a high speed, and then add water to the above solution in a homogenizer to make up to the final volume while stirring at a high speed.

Example 2

The specific operation steps are consistent with the above-mentioned Example 1, except that its formula (concentration by mass percentage) is composed of the following components: 0.001% thymol, 0.01% monoglyceride laurate, 1.5% calcium propionate, 1% dehydrated alcohol, water balance.

Example 3

The specific operation steps are consistent with the above-mentioned Example 1, except that its formula (concentration by mass percentage) is composed of the following components: 0.006% thymol, 0.02% monoglyceride laurate, 1% calcium propionate, 1% calcium formate, lemon Acid 0.01%, absolute ethanol 4%, water balance.

Example 4

The formula (concentration by mass percentage) of disinfectant is to be made up of following components:

Thymol 0.06%;

Monolaurin 0.1%;

Citric acid 0.4%;

Anhydrous ethanol 0.5%;

water balance.

The preparation method of disinfectant is as follows:

1. Weigh each component according to the formula amount, first dissolve thymol in a small amount of co-solvent absolute ethanol in the first container, stir at high speed until completely dissolved;

2. Take the second container and weigh monoglyceride laurate, add absolute ethanol to help dissolve;

3. Weigh the citric acid in the third container, add a small amount of water to dissolve, stir evenly at high speed, pour the solution in the second container into the first container, strengthen the stirring until it is completely uniform, measure the pH value, and then add citric acid as appropriate Adjust the pH value of the solution to 3-5, and fill it.

Comparative example 1

The specific operation steps are consistent with the above-mentioned embodiment 4, except that the content of thymol in the formula is 0.1%.

Comparative example 2

The specific operation steps are consistent with the above-mentioned embodiment 4, except that the content of thymol in the formula is 0.0006%.

Application example

Add thymol 0.015% (0.15kg), laurate monoglyceride 0.015% (0.15kg), calcium formate 0.015% (0.15kg), calcium propionate 0.015% (0.15kg), citric acid 0.015% ( 0.15kg), lactose 0.015% (0.15kg), hawthorn powder 0.01% (0.1kg), licorice powder 0.01% (0.1kg), each component is mixed in proportion.

Stability detection of experimental example 1 disinfectant

The appearance and stability of the disinfectants prepared by the above-mentioned examples and comparative examples are evaluated, and the method for specifically measuring the stability is: add the compound disinfectant prepared by the above-mentioned examples and comparative examples to 300ml of standard hard water, shake Evenly, let stand at 30°C for 30 minutes, the specific results are shown in Table 1 below.

Table 1 Test results

Bacteriostasis test of experimental example 2 disinfectants

The disinfectant of Example 2 and Comparative Example 1-2, the inhibitory effect of a single component on common pathogens (Escherichia coli, Staphylococcus aureus) and common probiotics (Bacillus subtilis) were measured.

Method: Use 1ml of nutrient medium to culture the disinfectant and 1m of the single component separately, inoculate the rejuvenated Escherichia coli, Staphylococcus aureus, and Bacillus subtilis respectively, inoculate them on nutrient agar plates and culture them at 37°C for 12- 24 hours, observe the result. The positive control plate had bacterial growth, and the negative control plate had no bacterial growth.

Table 2 Bacteriostatic effect evaluation

As can be seen from the above Table 2, one-component thymol and one-component lauric acid are insoluble in water, have weak antibacterial activity at low concentrations, and monoglyceryl laurate is insensitive to Escherichia coli. Low concentrations of citric acid and ethanol have no obvious antibacterial effect. After the microemulsion system is made into each single formula, they play a synergistic effect well, and the antibacterial ability of the system is enhanced under acidic conditions.

The formulation optimization of experimental example 3 disinfectant

1. Thymol inhibitory concentration

Thymol has a strong inhibitory effect on bacteria, mold, and yeast, among which the inhibitory effect on bacteria is the strongest. The minimum inhibitory mass concentration of thymol to bacteria was between 10-160 μg/mL.

2. Inhibitory concentration of monoglyceride laurate

Monolauric acid monoglyceride is unknown to Escherichia coli, Salmonella minnesota, Pseudomonas aeruginosa, Enterobacter aerogenes, Proteus, Shigella sordii, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus , Listeria monocytogenes, Bacillus anthracis, Clostridium perfringens, Candida albicans and other 39 common bacteria have minimum inhibitory concentrations between 1-500μg/mL.

3. The bacteriostatic concentration of citric acid is between 0.625-20mg/mL.

4. Taking thymol, monoglyceride laurate and citric acid as three factors and three levels of orthogonal experiment.

Specifically, based on Example 4, other operating steps are consistent with Example 4, except that the contents of thymol, monoglyceryl laurate, and citric acid are changed, as shown in Table 3-4 below.

Table 3 Compound disinfectant screening test factor level table

Table 4 antibacterial results

Test No. Citric acid (W/V) Lauric Monoglyceride (W/V) Thymol (W/V) Concentration after inhibition (cfu/ml) 1 1 1 1 0 2 1 2 2 10² 3 1 3 3 10⁴ 4 2 1 2 10³ 5 2 2 3 10⁴ 6 2 3 1 10 7 3 1 3 10⁶ 8 3 2 1 0 9 3 3 2 10⁷

Through the statistical analysis of the data in the above table, from the results of the average analysis, the level 1 of citric acid, the level 2 of monoglyceride laurate, and the level 1 of thymol should be selected.

It can be seen from the results of the variance that the results of the three are not significant (Pvalues are all greater than 0.05), but the Pvalue of citric acid is the smallest, so the selection of citric acid is the most important, and monoglyceryl laurate is relatively close to thymol. Citric Acid Level 1, Monolaurate Level 2, Thymol Level 1 are not available in our experimental design, either Citric Acid Level 1, Monolaurate Level 2, Thymol Level 2 or Citric Acid Level 1, Monolaurate Level 1, Thymol Level 1.

While particular embodiments of the invention have been illustrated and described, it should be appreciated that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.

Claims (10)

1. A disinfectant, characterized in that, comprises active ingredient, acidulant and water; The active ingredients include: 0.001% to 0.06% of thymol, 0.01% to 0.1% of monoglyceride laurate, and 0.5% to 5% of absolute ethanol in terms of mass percentage of the disinfectant; Wherein, the acidifying agent is selected from at least one of: 0.01-0.4% citric acid, 1%-1.5% calcium formate, and 1%-1.5% calcium propionate, based on the mass percentage of the disinfectant. 2. disinfectant according to claim 1, is characterized in that, described acidulant accounts for 0.01～0.4% of citric acid in the mass percent of described disinfectant, calcium formate 1%～1.5% and calcium propionate 1% % ~ 1.5%. 3. The disinfectant according to claim 1, characterized in that, the acidulant is 0.01-0.4% citric acid. 4. The disinfectant according to any one of claims 1-3, characterized in that, in terms of mass percentage, 0.006% to 0.03% of thymol, 0.02% to 0.06% of monoglyceride laurate, and 0.04% to 0.06% of citric acid 0.2%, absolute ethanol 1% to 4%, and the balance is water. 5. according to the described disinfectant of any one of claim 1-3, it is characterized in that, by mass percent, thymol 0.06%, lauric monoglyceride 0.05%, citric acid 0.4%, dehydrated alcohol 0.5%, The balance is water. 6. according to the described disinfectant of any one of claim 1-3, it is characterized in that, by mass percent, thymol 0.06%, lauric monoglyceride 0.1%, citric acid 0.4%, dehydrated alcohol 0.5%, The balance is water. 7. according to the preparation method of the disinfectant described in any one of claim 1-6, it is characterized in that, comprises the steps: (A) stirring and dissolving thymol and absolute ethanol to obtain the first mixed solution; (B) stirring the solution with monoglyceride laurate and the remaining amount of absolute ethanol to obtain the second mixed solution; (C) A third mixed liquid is formed after the acidulant is mixed and dissolved with a small amount of water; Wherein steps (A), (B) and (C) have no sequence; (D) Mix the first mixed liquid, the second mixed liquid and the third mixed liquid evenly, and add water to the final volume. 8. the preparation method according to claim 7, is characterized in that, in described step (D), described mixing is carried out by high-speed stirring in homogenizer; Preferably, the second mixed liquid is mixed with the first mixed liquid and then mixed with the third mixed liquid. 9. A feed additive, characterized in that it comprises the disinfectant according to any one of claims 1-6 or the disinfectant obtained by the preparation method according to any one of claims 7-8; Preferably, the feed additive further includes hawthorn, licorice and lactose. 10. the application of the disinfectant described in any one of claims 1-6 in livestock and poultry disinfection, drinking water disinfection, environmental disinfection.