CN110199944B - A method for testing the attention of experimental animals by using a transparent rotating ball and a visual attractant - Google Patents

Abstract

The invention relates to a method for testing the attention of experimental animals by using transparent rotary balls and visual attractants. The method comprises the following steps: (A) A transparent sphere which can rotate with low resistance or can freely roll within a certain plane range and can accommodate experimental animals and has sufficient experimental animal activity space is arranged, and a vent hole is arranged on the side surface or the surface of the transparent sphere; (B) Visual attractants are arranged on the outer surface or the outer surface of the transparent sphere at a certain distance, the visual attractants are fixed or can randomly move or set a movement mode, and the attractants are closed or not closed; (C) Placing an experimental animal in a transparent sphere, and beginning to record the time of continuous movement of the animal facing the attractant; (D) After each test the transparent spheres were thoroughly washed to eliminate olfactory cues. The attractant is sealed to isolate smell, and the attention is directly measured; the attractants were not occluded to determine concentration including attention. The invention relates to a method for testing the attention of experimental animals, which is simple and visual to operate.

Description

A method for testing the attention of experimental animals by using a transparent rotating ball and a visual attractant

technical field

The invention relates to a method for testing the attention of experimental animals by utilizing a transparent rotating ball and a visual attractant. It can be applied to test the influence of food and medicine on the attention of experimental animals, and belongs to the field of food and medicine processing.

Background technique

The present invention designs a method for testing the attention of experimental animals by using transparent rotating balls and visual attractants. The method is simple and direct, and can be used to test the attention and concentration of experimental animals by eating food, medicine or injection liquid. Influence;

preliminary test. Contents include swimming test and rotating cage test for endurance or anti-fatigue test, various maze tests for learning and memory ability test, tail suspension test for anxiety suppression test, open field test for neuropsychiatric test, CPA conditioned aversion system for drug addiction test Gait Behavior Analysis System Trial, Motor Coordination Test. It provides an objective, intuitive and easy-to-use observation method for the development of food and drug products, and is widely used in related research and development at home and abroad;

Attention-related problems such as ADHD in children, neurasthenia in adults, and the decline in students' learning ability have attracted widespread attention from the society, but researchers have not paid attention to them or taken effective countermeasures for a long time. Mainly, there is no simple and easy way to test attention. An alternative method can be to use EEG observation method, but this method is costly, and the observation indicators are not intuitive or difficult to determine attention.

Contents of the invention

The purpose of this invention is to provide a kind of method that utilizes transparent rotating ball and visual attractant to test experimental animal's attention,

The purpose of the present invention is achieved by the following methods:

A. Set up transparent spheres that can rotate with low resistance or can roll freely within a certain plane range, which can accommodate experimental animals and have sufficient space for experimental animals to move. Set ventilation holes on the side surface or surface of the transparent sphere, and reserve movable doors;

B. Set visual attractors on the outer surface of the transparent sphere or at a certain distance outside, the visual attractors are fixed or can move randomly or set the movement mode, and the attractors are closed or not;

C. Place the experimental animal in the transparent sphere, and start recording the time that the animal continues to move towards the lure;

D. The transparent spheres were thoroughly washed after each test to eliminate olfactory cues. The attractant is closed to isolate the sense of smell and directly measures attention; the unblocked attractant is used to measure the degree of concentration including attention.

The low-resistance rotation means that the device can rotate around a fixed axis;

The transparency of the transparent sphere is based on the fact that the sphere material replaces the spectacle lens, and the human vision is greater than 0.1;

The above-mentioned visual attractants include different colors, sound and light graphics or toys, materials of different colors or smells;

The random movement mentioned above means that the lure can move irregularly under the condition of direct or indirect collision with the transparent sphere;

The set motion mode includes circular, up and down, front and back, left and right swinging motion modes;

The above-mentioned continuous activity refers to the continuous activity that the temporary suspension of activity induced by the attractant does not exceed the set interval;

The elimination of olfactory hints refers to the elimination of olfactory residues in the device caused by different experimental animals;

The degree of concentration includes the attention caused by vision, the attention caused by smell and hearing.

The invention uses a method for testing the attention of experimental animals by using a transparent rotating ball and a visual attractant, which is simple, intuitive and easy to operate, and each step of the invention constitutes a complete technical solution.

Detailed ways

The following examples are used to illustrate the present invention and make it more definite, but in no way limit the scope of the present invention,

Example 1:

1) Put the mouse in a plastic transparent rotating ball placed on a rotating stand, let it adapt to the internal environment for 6 hours, and return it to its original place;

2) Thoroughly clean the inside and outside of the plastic transparent rotating ball with cleaning solution;

3) Suspend the bulk feed sealed in the glass ball at a distance of 20 cm from the transparent rotating ball;

4) After 24 hours, put the mouse into the plastic transparent rotating ball on the rotating stand, and start recording its continuous activity time (s, seconds), and the temporary suspension time of no more than 1 s is regarded as continuous activity;

5) Repeat the above steps for three consecutive days, measure once a day, and take the average of the continuous activity time as the attention test index.

Claims (9)

1. A method for testing the attention of experimental animals by using transparent rotary balls and visual attractants, which is characterized by comprising the following steps:

A. a transparent sphere which can rotate with low resistance or can freely roll in a certain plane range and can accommodate experimental animals and has enough experimental animal moving space is arranged, a vent hole is arranged on the side surface of the transparent sphere, and a movable door is reserved;

B. visual attractants are arranged on the outer surface or the outer surface of the transparent sphere at a certain distance, the visual attractants are fixed or can randomly move or set a movement mode, and the attractants are closed or not closed;

C. placing an experimental animal in a transparent sphere, and beginning to record the time of continuous movement of the animal facing the attractant;

D. after each test the transparent spheres were thoroughly washed to eliminate olfactory cues.

2. A method of testing the attention of laboratory animals using a transparent rotating ball and visual attractants according to claim 1, wherein the low resistance rotation of step a is such that the transparent ball rotates about a fixed axis.

3. The method of claim 1, wherein the transparent sphere in step a has a transparency of greater than 0.1 as a reference for replacing the spectacle lenses with sphere materials.

4. A method for testing the attention of an experimental animal by using a transparent rotary ball and a visual attractant as in claim 1, wherein the visual attractant in step B comprises figures of different colors and sound and light.

5. A method of testing the attention of laboratory animals using transparent rotating balls and visual attractants as claimed in claim 1 wherein the random movement of step B directs the attraction to move irregularly under direct or indirect collision conditions with the transparent ball.

6. The method of claim 1, wherein the set motion pattern in step B comprises up-down, back-and-forth, side-to-side, and rotational motion patterns.

7. A method of testing the attention of an experimental animal using a transparent rotating ball and a visual attractant as in claim 1, wherein the attractant is enclosed in step B, wherein the attractant is enclosed for direct measurement of attention.

8. A method of testing the attention of an experimental animal using a transparent rotating ball and a visual attractant as in claim 1, wherein the attractant of step B is unsealed and the attractant is unsealed for determining the concentration of the containing attention.

9. A method of testing the attention of laboratory animals using transparent rotary spheres and visual attractants as in claim 1 wherein the continuous activity of step C is one in which the temporary cessation of activity induced by the attractants does not exceed a set interval.