CN110178838A - A kind of production method of green plants plasticizing sample - Google Patents

Abstract

The present invention relates to a kind of production methods of green plasticizing sample comprising following steps: (1) prepared by plastifying liquid: PEG being configured to certain density plastifying liquid, sorbierite is added, dissolution adjusts plastifying liquid pH=6-8, spare；(2) it is plasticized pre-treatment: the fresh green plant of acquisition being cleaned and is dried, carry out pure water and bathe immersion treatment；(3) it is plasticized: treated plant is all soaked in the plastifying liquid got ready, place and be plasticized in dark place, wipe plasticiser and obtain plasticizing sample；Method provided by the present invention is easy to operate, and manufacturing process is quick, safe and non-stimulating.Fusion cycle is short, can protect color by fidelity steadily in the long term.

Description

A kind of preparation method of plasticized specimen of green plant

technical field

The invention relates to a method for making green plasticized specimens, belonging to the technical field of plant preservation.

Background technique

Medicinal plant specimens are "Chinese Materia Medica", "Pharmaceutical Botany", "Chinese Medicine Identification", "Pharmaceutics" and other related disciplines. They are indispensable and important reference physical archives, and are an indispensable part of teaching and scientific research. . At present, common specimens are divided into the following three categories: wax leaf specimens, dipped specimens and plasticized specimens. Wax leaf specimen The wax leaf specimen is a plant specimen that has been pressed and dried. Its defects are easy to fade, break, and easy to be eaten by insects and mildew, so it is not suitable for long-term storage. Dipping specimens are obtained by immersing plants in a preservation solution after color-fixing treatment. The disadvantages are that the preservation solution is often poisonous, volatile, and has a pungent smell, which is not conducive to viewing and learning. Plasticized specimens are favored by people because of their pollution-free, vivid and other characteristics, and polyethylene glycol is used in the production of plasticized specimens because of its mildness, good compatibility, low toxicity and no irritation. Reports on plasticized specimens are as follows:

The publication number is CN1826887A, which discloses a plasticizing preparation method of animal and plant specimens, including sterilization, anticorrosion, cleaning and plasticizing processes. In the plasticizer polyethylene glycol, the moisture in the specimen is replaced by polyethylene glycol. Its production cycle is long, and the plasticization of plants has not been studied in depth.

The publication number is CN103548817A, which relates to a method for making plasticized plant specimens. The method includes the following plasticizing steps: soak the color-fixed plants in successively increasing solutions of 5 polyethylene glycol mass fractions, and each adjacent two The difference of the polyethylene glycol mass fraction of each solution is at 15%-30%, until the polyethylene glycol mass fraction of the last solution is 100%; the time of each soaking is controlled at 5-20 hours, and the last soaking The time is not less than the time of the previous soaking.

CN107047545A A method for plasticizing specimens of green plants and the application of the prepared specimens include the steps of color fixation and plasticization, and the plasticization is specifically as follows: soak green plants with good color fixation in 20% polyethylene glycol solution 10-15 hours, followed by soaking in 40% polyethylene glycol solution for 8-10 hours, 60% polyethylene glycol solution for 8-10 hours, and 80% polyethylene glycol solution for 8-10 hours.

The above methods are aimed at the plasticization of plants, and are generally carried out through two steps of color fixation and plasticization. During the curing process, inorganic solvents are used to treat plants, such as Cu ²⁺ acetic acid solution (providing acidic environment and Cu ²⁺ ), The principle is that the molecular structure of chlorophyll in leaves is a porphyrin ring complexing magnesium ions. In an acidic environment, H ⁺ will replace Mg ²⁺ to form magnesium chlorophyll, and the leaves are brown. Demagnesium chlorophyll is easy to combine with Cu ²⁺ to form With more stable copper-substituted chlorophyll, the leaves return to green. Glacial acetic acid is often used in the production process, which is irritating to the operator, and the excessive use of copper ions is not environmentally friendly. The plasticizing steps are all plasticized by gradually immersing in gradient polyethylene glycol, which has the defects of complicated experimental operation and long processing time.

Contents of the invention

The technical problem to be solved by the present invention is to provide a method for making green plasticized specimens with simple operation, short plasticizing cycle, long-term stability, fidelity and color preservation.

In order to solve the problems referred to above, the technical scheme that the present invention takes is:

A method for making a green plasticized specimen, comprising the steps of:

(1) Preparation of plasticizing solution: prepare PEG into a certain concentration of plasticizing solution, add sorbitol, dissolve, adjust the pH of the plasticizing solution to 6-8, and set aside;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and soak them in a pure water bath;

(3) Plasticization: Soak all the treated plants in the prepared plasticizing solution, place them in a dark place for plasticization, and wipe off the plasticizer to obtain plasticized specimens.

In some embodiments of the present invention, the PEG configuration concentration in step (1) is 40wt%-60wt%.

In some embodiments of the present invention, the PEG in step (1) is PEG200-600.

In some embodiments of the present invention, the solvent of the plasticizing solution in step (1) is degassed pure water. In some embodiments of the present invention, the addition ratio of sorbitol in step (1) is 0.1-0.3g/5ml.

In some embodiments of the present invention, the temperature of the pure water bath in step (2) is 50-60°C, and the treatment time is 60s-120s.

In some embodiments of the present invention, the pure water bath in step (2) is a degassed pure water bath.

In some embodiments of the invention, the steps are as follows:

(1) Preparation of plasticizing solution: Prepare PEG200-600 and degassed distilled water to form a 40%-60% plasticizing solution, add sorbitol at a ratio of 0.1-0.3g/5ml, dissolve, and adjust the pH of the plasticizing solution =6-8;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 50-60°C for 60s-120s;

(3) Plasticization: Soak all the treated plants in the new plasticizing solution, place them in a dark place for 24 hours, wipe off the plasticizer to obtain plasticized specimens.

The beneficial effects produced by adopting the above-mentioned technical scheme are:

The method provided by the invention is simple to operate, and the production process is fast, safe and non-irritating. The plasticizing cycle is short, and it can be stable for a long time with fidelity and color.

The invention controls the factors affecting the decomposition of chlorophyll in the color fixing stage, so that the chlorophyll in the green plant specimen can be preserved. Before plasticizing, it is treated with a degassed pure water bath at a certain temperature to effectively destroy chlorophyllase, delay plant senescence, yellow leaves and other plant physiological phenomena, and complete plant color fixation. This method is economical and environmentally friendly, and does not involve the replacement process of metal ions.

The invention utilizes the degassed plasticizing liquid in which sorbitol and polyethylene glycol are simultaneously dissolved for processing in the plasticizing process, the time is short, the plasticizing can be quickly realized, and the manufacturing method is simpler and quicker.

Description of drawings

In order to more clearly illustrate the effects of specific implementations of the present invention or technical solutions in the prior art, reference is provided to the accompanying drawings.

Fig. 1 is the plasticized specimen obtained by the method of Example 6 of the present invention.

Detailed ways

In order to make the object, technical solution and advantages of the present invention clearer, the invention will be clearly and completely described below in conjunction with specific embodiments.

In the following examples, the reagents used for pH adjustment are 5% KOH and 0.1M HCl.

Example 1

(1) Preparation of plasticizing solution: Prepare PEG200 and degassed distilled water to form a plasticizing solution of about 40wt%, and add sorbitol at a ratio of 0.1g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pretreatment of plasticization: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 50±1°C for 60 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 2

(1) Preparation of plasticizing solution: Prepare PEG200 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.1g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: Wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 50±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 3

(1) Preparation of plasticizing solution: Prepare PEG200 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 50±1°C for 120s;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 4

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 40wt%, and add sorbitol at a ratio of 0.1g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pretreatment of plasticization: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 60 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 5

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.1g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 6

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pretreatment of plasticization: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 120s;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 7

(1) Preparation of plasticizing solution: Prepare PEG600 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.1g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pretreatment of plasticization: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 60 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 8

(1) Preparation of plasticizing solution: Prepare PEG600 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 9

(1) Preparation of plasticizing solution: Prepare PEG600 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pretreatment of plasticization: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 120s;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 10

(1) Preparation of plasticizing solution: Prepare PEG200 and degassed distilled water to form a plasticizing solution of about 40wt%, and add sorbitol at a ratio of 0.3g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: Wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 60±1°C for 60 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 11

(1) Preparation of plasticizing solution: Prepare PEG200 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.3g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pretreatment of plasticization: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 60±1°C for 120s;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 12

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.3g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: Wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 60±1°C for 60 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 13

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 40wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pretreatment of plasticization: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 60±1°C for 120s;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 14

(1) Preparation of plasticizing solution: Prepare PEG600 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.3g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: Wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 60±1°C for 60 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 15

(1) Preparation of plasticizing solution: Prepare PEG600 and degassed distilled water to form a plasticizing solution of about 40wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pretreatment of plasticization: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 60±1°C for 120s;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 16

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 40wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 6;

(2) Pretreatment of plasticization: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 60 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Example 17

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 60wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 8;

(2) Pretreatment of plasticization: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 120s;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 1

(1) Preparation of plasticizing solution: prepare PEG400 and degassed distilled water to form a plasticizing solution of about 50wt%, and adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 2

(1) Preparation of plasticizing solution: prepare PEG400 and degassed distilled water to form a plasticizing solution of about 50wt%, add glycerin at a ratio of 0.2g/5ml, and adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 3

(1) Preparation of plasticizing solution: prepare PEG400 and degassed distilled water to form a plasticizing solution of about 50wt%, add mannitol at a ratio of 0.2g/5ml, and adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 4

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 50wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Measure the pH to 4

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 5

(1) Preparation of plasticizing solution: Prepare PEG400 and water to form a plasticizing solution of about 50wt%, add sorbitol at a ratio of 0.2g/5ml, if sorbitol is not easy to dissolve, heat it in a water bath to dissolve it, and measure the pH to 10

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 6

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 30wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 7

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 70wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 8

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 50wt%, and add sorbitol at a ratio of 0.05g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 9

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 50wt%, and add sorbitol at a ratio of 0.4g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 55±1°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 10

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 50wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Plasticizing: Soak all the plants in the new plasticizing solution and place them in a dark place for 24 hours;

(3) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 11

(1) Preparation of plasticizing solution: Prepare PEG400 and degassed distilled water to form a plasticizing solution of about 50wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath. Adjust the pH of the plasticizing solution to 7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 45°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 12

(1) Preparation of plasticizing solution: Prepare PEG400 and water to form a plasticizing solution of about 50wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath to adjust the plasticizing solution pH=7;

(2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 65°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Comparative example 13

(1) Preparation of plasticizing solution: Prepare PEG400 and non-degassed distilled water to form a plasticizing solution of about 50wt%, and add sorbitol at a ratio of 0.2g/5ml. If sorbitol is not easy to dissolve, it can be dissolved by heating in a water bath and adjusted. Plasticizing solution pH=7;

(2) Pre-treatment of plasticization: wash and dry the collected fresh green plants, and place them in a pure water bath without degassing at 55°C for 90 seconds;

(3) Plasticization: Soak all the above-mentioned treated plants in the new plasticizing solution, and place them in a dark place for 24 hours;

(4) Preservation: Take out the plasticized specimen, wipe off the plasticizing solution, put it into a specimen bottle, and store the specimen in a dark place when not in use.

Effect example 1 high temperature test

The specimens obtained in Examples 1-17 and Comparative Examples 1-13 were placed at a temperature of 55±2° C. for 72 hours, and the morphology of the specimens was investigated. The results are shown in Table 1.

Table 1 High temperature test results

Effect example 2 High humidity test result

For the specimens obtained in Examples 1-17 and Comparative Examples 1-12, place them for 7 days at a temperature of 40 ± 2°C and a humidity of 93 ± 3%, and investigate the morphology of each specimen. The results are shown in Table 2

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: it can still be Modifications are made to the technical solutions described in the foregoing embodiments, or equivalent replacements are made to some of the technical features; these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (8)

1. a preparation method of green plasticized specimen, is characterized in that, it comprises the steps: (1) Preparation of plasticizing solution: prepare PEG into a certain concentration of plasticizing solution, add sorbitol, dissolve, adjust the pH of the plasticizing solution to 6-8, and set aside; (2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and soak them in a pure water bath; (3) Plasticization: Soak all the treated plants in the prepared plasticizing solution, place them in a dark place for plasticization, and wipe off the plasticizer to obtain plasticized specimens. 2. A kind of preparation method of green plasticized specimen according to claim 1, is characterized in that, in step (1), PEG configuration concentration is 40wt%-60wt%. 3. The preparation method of a kind of green plasticized specimen according to claim 1, characterized in that, in the step (1), PEG is PEG200-600. 4. The preparation method of a kind of green plasticized specimen according to claim 1, characterized in that, the solvent of the plasticized liquid in the step (1) is degassed pure water. 5. The preparation method of a kind of green plasticized specimen according to claim 1, characterized in that, the addition ratio of sorbitol in step (1) is 0.1-0.3g/5ml. 6. The method for making a green plasticized specimen according to claim 1, wherein the temperature of the pure water bath in step (2) is 50-60° C., and the processing time is 60s-120s. 7. The preparation method of a kind of green plasticized specimen according to claim 1, characterized in that, the pure water bath is a degassed pure water bath in the step (2). 8. the preparation method of a kind of green plasticized specimen according to claim 1, is characterized in that, the steps are as follows: (1) Preparation of plasticizing solution: Prepare PEG200-600 and degassed distilled water to form a 40%-60% plasticizing solution, add sorbitol at a ratio of 0.1-0.3g/5ml, dissolve, and adjust the pH of the plasticizing solution =6-8; (2) Pre-plasticization treatment: wash and dry the collected fresh green plants, and place them in a degassed pure water bath at 50-60°C for 60s-120s; (3) Plasticization: Soak all the treated plants in the new plasticizing solution, place them in a dark place for 24 hours, wipe off the plasticizer to obtain plasticized specimens.