CN110178760A - The method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population breeds high feminisation seed - Google Patents
The method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population breeds high feminisation seed Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The present invention relates to the technical field of Cynoglossus semilaevis breeding, specially a kind of methods that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population breeds high feminisation seed.Including parental source and select, parent fish rearing, artificial induced spawning and insemination and hatching, seed rearing, the present invention regulates and controls spawning and fertilization by Optimum Regulation mode, perinereis aibihitensis Grube enriched nutritive, temperature, rate of fertilization is 83.5%, hatching rate 74.6%, female-male proportion 5:2.The present invention solves the problems, such as that raiser's goes out female rate, and the aquaculture cost of raiser is greatly saved, brings the huge interests on direct economy for numerous raisers, further promotes the development of Cynoglossus semilaevis industrial aquaculture industry.
Description
Technical field
The present invention relates to the technical field of Cynoglossus semilaevis breeding, specially a kind of nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent populations
The method for breeding high feminisation seed.
Background technique
Cynoglossus semilaevis is the important economic seawater fish of northern China, fine and tender taste, delicious flavour, full of nutrition, is gone through
Deep to be favored come the first-class good merchantable brand received guests for China coast the majority of consumers, northern common saying " spring flower autumn sole " just refers to that autumn eats
With the meaning of tongue sole cuisines.In recent years, there are the events such as turbot drug disturbance and rushes to seawater fish market in marine fish culture
Very big, many marine fishes prices depressions are hit, and Cynoglossus semilaevis is constantly in the column of high-level fish, by market clout very little, edge
In its huge market demand, quantity of the catch is small, is unable to satisfy the market demand；Although artificial breeding and forming carried out for many years, by
In raising technology hardly possible, seed male ratio height causes final commodity fish crop not high, is unable to satisfy the market demand always.
The Cynoglossus semilaevis excellent fish culture kind newly developed as current China, in terms of the current market demand, in valence
It is advantageous on lattice, and be also very famous in terms of the consumption habit of consumer and consumer psychology.This is cut to Cynoglossus semilaevis rule
Modelling, industrialization cultivation are laid a good foundation, and industrialization pattern is gradually forming, boundless at present, it is contemplated that half is sliding
This emerging sea-farming type of tongue sole will start the sea-farming tide of a new round after turbot cultivates.Due to half
The factor of sliding-tongue sole autogene, male and female individual difference is larger, and the speed of growth is as different as light from darkness, and raiser is to Cynoglossus semilaevis
Female rate that goes out especially be concerned about, and be related to the cultivation interests of itself, it can be seen that lay eggs to the parent population of Cynoglossus semilaevis this kind
The research of amount, fertilization and hatching rate and high feminizing techniques is particularly important.
Mainly in China, external cultivation is Europe sole for the cultivation of Cynoglossus semilaevis, not the cultivation situation of Cynoglossus semilaevis.Mesh
Before, North gets Liaoning, Hebei, south arrives Guangdong, Hainan Island, there is the cultivation for being engaged in Cynoglossus semilaevis.The seed rearing master of Cynoglossus semilaevis
Based on being cultivated with batch production workshop, up to the present, annoying always in relation to the problem of semi-smooth tongue sole offspring breed female-male proportion feeding
Family is grown, is not also found delivering for related academic paper.
Summary of the invention
High insufficient for semi-smooth tongue sole offspring breed male ratio in the prior art, the present invention provides a kind of nutrition regulation HUANGHE ESTUARY
The method that Cynoglossus semilaevis parent population breeds high feminisation seed, to solve the above technical problems.
The present invention provides a kind of method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population breeds high feminisation seed, comprising: parent
It this source and selects, parent fish rearing, artificial induced spawning and insemination and hatching, seed rearing:
(1) parental source with select: select 3-5 age Cynoglossus semilaevis parent population, at 1500 grams or more, milter exists raun weight
200 grams or more, female-male proportion 1:5, further include wild Cynoglossus semilaevis parent population to guarantee kind of a fish purity.
(2) parent fish rearing: monitoring index includes temperature, salinity, dissolved oxygen, pH value, ammonia nitrogen routine water quality indicator, record
The diurnal variation of each physical and chemical index and season situation of change and with being adjusted, keep the stabilization of water quality；
Breeding density raun controls in 1-3 tail/m2, milter can be controlled in 5-7 tail/m2；
Intensity of illumination is controlled in 500-800lux, and light is uniform, soft；Light application time contracts daily since 18 hours
Light application time was stablized in 11-12 hour after the short photoperiod time 5 minutes, 90 days；
It cultivates and uses water level sand leaching water, water body is as clear as crystal；Water temperature: 21-25 DEG C；Salinity: 28-32 ‰；PH value: 7.8-8.6；
Dissolved oxygen: 6mg/L or more；Ammonia nitrogen: less than 0.1mg/L；
It is fed with fresh and alive perinereis aibihitensis Grube, and suitably feeds mixed feed, fed daily 2 times, each 1 time sooner or later, thrown
Bait amount is the 1-3% of fish body weight, daily clear bottom 1 time, and discharge water big blowdown 2 times, and quantity of exchanged water is no less than 8 circulations (800%) daily.
(3) artificial insemination: female parent fish selection being come out and carries out injection of hormone, and hormone is that luteinizing hormone releasing hormone is similar
The mixing of object (LRHL-A3) and human chorionic gonadtropin (HCG)；
Intramuscular injection is used to female parent population with HCG/ kilograms of international unit of 5 microgram LRHL-A3+50 of dosage；
The ovum extruded is mixed with sperm, after being gently mixed half a minute with hand, the water that fish-egg equivalent weight is added continues
Half a minute is stirred, when stirring notices that hand cannot encounter chamber wall, and to stir evenly, static to be washed after twenty minutes with clean seawater
Only, hatch；
Static 20 minutes after fertilized eggs are cleaned, the fertilized eggs for choosing transparent floating are weighed, hatching, and general use is educated
The 80 micro- Lotic hatchings of mesh net cage, hatching density 500g/m are placed in Miao Chi3, 23 DEG C of water temperature of hatching, salinity 30 ‰, illumination
500lux, silent as the grave ovum in each 2 removing net cages, micro- inflation sooner or later, carries out cloth pond after hatching 36h daily.
(4) seed rearing: cloth ovum density is 10g/m3, 23 DEG C of water temperature, salinity 30 ‰, pH8.0-8.4, dissolved oxygen 5mg/L
More than, ammonia nitrogen is less than 0.1mg/L, intensity of illumination 2000lux；
Cloth pond after hatching 36h, the same day can hatch rupture of membranes, not need to feed within first 3 days, to the 4th day progress chlorella and wheel animalcule
Feed, the density that feeds of chlorella is 2,000,000/ml, and it is 5-8/ml that wheel animalcule, which feeds density, and the 10th day starts to feed artemia
Nauplius, wheel animalcule are suitably reduced, and it is 0.5-1/ml that halogen children, which feeds density, and wheel animalcule stops throwing within the 15th day, and halogen children increases and feeds 1-2
A/ml gradually started metamorphosis by the 18th day, and metamorphosis in the 24th day is basically completed, and fry is in the attached wall-like state in attached bottom, into postlarva
The feeding volume of phase, halogen children continue to increase, and when 2.5-3cm gradually changes throwing artemia imago, and 3.5-4cm starts to tame mixed feed, wheel
Worm, halogen children need to carry out fortification before feeding.
Further, in step (2), water temperature is preferably 23 DEG C.
Further, in step (2), warm light regulation 2-4 months, preferably 3 months.
Further, in step (1), the accounting of wild Cynoglossus semilaevis parent population is 50%.
Further, in step (4), start within the 6th day to change water, daily 10cm, to quantity of exchanged water when being increased when feeding halogen children, often
It 1-2 circulation (100-200%), when feeding artemia imago, the daily 3-4 circulation (300-400%) of quantity of exchanged water feeds cooperation and raises
It, daily will be more than 5 circulations (500%) when material.
Further, in step (4), after feeding artemia imago, clear bottom is primary daily, and blowdown is twice.
Further, in step (4), when 2.5-3cm, breeding density is in 2000 tails/m2, when 3.5-4cm, breeding density exists
1000 tails/m2, when 5-8cm, breeding density is in 500 tails/m2。
The beneficial effects of the present invention are,
The method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population provided by the invention breeds high feminisation seed,
Water temperature, light application time etc. using the development models of Cynoglossus semilaevis parent population in nature, in simulating natural environment
To carry out parent population warm light regulation, so that parent population be made to reach certain accumulated temperature product light, carries out gonad development.Using parent population oviposition by
To the regulation of temperature when smart, 23 degree of optimum temperature are obtained, seed female-male proportion is made to improve 30%.
Nutrient required for being developed using parent population is fed to carry out nutrition addition, with the unsaturation in perinereis aibihitensis Grube
Amino acid and must amino acid come required nutrition when supplementing Cynoglossus semilaevis development, sought required for parent population when development to reach
It supports.
By it is artificial squeeze ovum fertilization in the way of to each temperature when fertilized eggs carry out respectively cultivate comparison, obtain rate of fertilization,
Relationship between hatching rate and female-male proportion etc. obtains high feminisation seed to obtain optimal incubation condition.
In short, the present invention passes through Optimum Regulation mode, perinereis aibihitensis Grube enriched nutritive, temperature regulation spawning and fertilization, fertilization
Rate is 83.5%, hatching rate 74.6%, female-male proportion 5:2.The present invention solves the problems, such as that raiser's goes out female rate, saves significantly
The aquaculture cost of raiser has been saved, the huge interests on direct economy has been brought for numerous raisers, it is sliding further to promote half
The development of tongue sole industrial aquaculture industry.
(1) Cynoglossus semilaevis parent population is cultivated using fortification bait, obtains fertilized eggs 8.3kg, and rate of fertilization is mentioned from 53%
For height to 83.5%, hatching rate is increased to 74.6% from 40%.
Specific embodiment
Technical solution in order to enable those skilled in the art to better understand the present invention, below in conjunction with of the invention real
Example is applied, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is only
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without making creative work, should fall within the scope of the present invention.
Embodiment 1
1 environmental condition
1.1 sanitary landifll
Selection seawater has abundant water resources, is pollution-free, draining is convenient, underground water source is sufficient, has a good transport service, and electric power abundance and has light
Field is built in the area at water water source.
1.2 condition of water quality
Source quality meets the regulation of GB/11607, and cultivation water meets the regulation of NY5052.
2 kinds, Miao Peiyu facility
Reservoir, sedimentation basin, sand filter (sandfiltration pot), ooxygenation facility, heating and cooling facility, parent fish rearing pond, seed rearing
Pond.
The selection of 3 parent populations
3.1 source
First is that the wild fish of natural waters fishing, can be used as parent population after domestication and fortification cultivate and uses.Two
It is that the selection speed of growth is fast in artificial breeding group, the excellent individual of growth properties is used as parent population.
3.2 appearance
Body colour is normal, physical strong, Burden-Swimming Ability of KM is strong, Full size, the individual with canonical biometric characteristic.
3.3 specifications and male and female match
The parent population in 3-5 ages is selected, at 1500 grams or more, milter is advisable raun weight at 200 grams or more, and female-male proportion is
1:5 is advisable.
The transport of 3.4 parent populations
Transport is stopped eating for first 48 hours.
12-14 DEG C of waters of transport temperature are the 1/4 of live fish bag.
With long 70cm, the single tail oxygenation transport of the live fish bag of wide 20cm.
After parent population is transported back, the temperature difference is less than ± 2 DEG C in culture pond, and salinity difference is less than ± 5 ‰.
4 parent fish rearings
4.1 culture pond
Culture pond is that round pan bottom shape is advisable, and discharge outlet is set among bottom of pond, slope ratio is advisable for 30%, bottom of pond preferably brush aquatic products
Dedicated white environmental protection paint, 1.0-1.5m of Chi Shen are advisable.
4.2 cultivating and managing
4.2.1 density
Breeding density should not be too large, and male and female, which separate, individually cultivates, and raun can be controlled in 2 tails/m2, milter can be controlled in 6 tails/
m2。
4.2.2 intensity of illumination and time
Intensity of illumination is controlled in 500-800lux, and light is uniform, soft；Light application time contracts daily since 18 hours
Light application time was stablized in 11-12 hour after the short photoperiod time 5 minutes, 90 days.
4.2.3 environmental condition:
Cultivation water must use sand leaching water, and water body is as clear as crystal.
Water temperature: 23 DEG C are advisable.
Salinity: 28-32 ‰.
PH value: 7.8-8.6.
Dissolved oxygen: 6mg/L or more.
Ammonia nitrogen: less than 0.1mg/L.
4.2.4 daily management
Based on fresh food (clam worm, shellfish and the chilled flesh of fish), and mixed feed is suitably fed, fed daily 2 times, it is early
Evening each 1 time, daily ration, feeding quantity is the 1-3% of fish body weight, daily clear bottom 1 time, and discharge water big blowdown 2 times, and quantity of exchanged water is no less than 8 daily and follows
Ring (800%).
5 parent population natural spawnings collect ovum, artificial induced spawning and insemination and hatching
5.1 natural spawning
Cynoglossus semilaevis belongs in batches mature, multiple Type of Spawning fish.When water temperature, illumination reache a certain level, female is close
Fish belly portion is gradually swelled, be in " carrot " shape, female milter is put into spawning pond in proportion at this time, milter have significantly chase it is female
The phenomenon that fish, when chasing to a certain extent, there is oviposition sperm drainage phenomenon in female milter, and ovum and sperm combine fertilization in water.
Natural spawning is relatively high to water quality requirement, as clear as crystal.
5.2 collect ovum
Naturally produce fertilized eggs needs artificially collect, first is that can by manually being fished for the pyrometric cone net of 100 mesh,
Second is that fertilized eggs are collected into the outer container of pond by spilling water method, manually incubated after the fertilized eggs being collected into are cleaned with clear water
Change.
5.3 artificial induced spawning and insemination
5.3.1 the artificial induced spawning time
It will be come out in the female parent fish selection of " carrot " shape and carry out injection of hormone.
5.3.2 oxytocic drug type
Luteotropin releasing hormone d-ala analog (LRHL-A3) and human chorionic gonadtropin (HCG) are used in mixed way.
5.3.3 dosage
5 HCG/ kilograms of international unit of microgram LRHL-A3+50
5.3.4 injecting method
Using intramuscular injection, milter does not have to injection.
5.3.5 artificial insemination
The ovum extruded is mixed with sperm, after being gently mixed half a minute with hand, the water that fish-egg equivalent weight is added continues
Half a minute is stirred, when stirring notices that hand cannot encounter chamber wall, and to stir evenly, static to be washed after twenty minutes with clean seawater
Only, hatch.
5.4 hatching
Static 20 minutes after fertilized eggs are cleaned, the fertilized eggs for choosing transparent floating are weighed, hatching, and general use is educated
The 80 micro- Lotic hatchings of mesh net cage, hatching density 500g/m3,23 DEG C of water temperature of hatching, salinity 30 ‰, illumination are placed in Miao Chi
500lux, daily silent as the grave ovum in each 2 removing net cages sooner or later, micro- inflation.Cloth pond is carried out after hatching 36h.
6. seed rearing
6.1 cloth ponds
By seed cultivation pool disinfection, clean, full pool spilling head after the clean seawater to full water of addition sand filtration before cloth pond
EDTA5ppm, micro- inflation, cloth ovum density are 10g/m3。
6.2 seed rearing conditions
23 DEG C of water temperature, salinity 30 ‰, pH8.0-8.4, dissolved oxygen 5mg/L or more, ammonia nitrogen is less than 0.1mg/L, intensity of illumination
2000lux。
6.3 larval rearing
Cloth pond after hatching 36h, the same day can hatch rupture of membranes, not need to feed within first 3 days, to the 4th day progress chlorella and wheel animalcule
Feed, the density that feeds of chlorella is 2,000,000/ml, and it is 5-8/ml that wheel animalcule, which feeds density, and the 10th day starts to feed artemia
Nauplius, wheel animalcule are suitably reduced, and it is 0.5-1/ml that halogen children, which feeds density, and wheel animalcule stops throwing within the 15th day, and halogen children increases and feeds 1-2
A/ml gradually started metamorphosis by the 18th day, and metamorphosis in the 24th day is basically completed, and fry is in the attached wall-like state in attached bottom, into postlarva
The feeding volume of phase, halogen children continue to increase, and when 2.5-3cm gradually changes throwing artemia imago, and 3.5-4cm starts to tame mixed feed, wheel
Worm, halogen children need to carry out fortification before feeding.
6.4 larva and juvenile period managements
6.4.1 quantity of exchanged water
Start within 6th day to change water, daily 10cm, to quantity of exchanged water when increasing when feeding halogen children, daily 1-2 circulation (100-
200%) when, feeding artemia imago, quantity of exchanged water daily 3-4 circulation (300-400%) daily will be at 5 when feeding mixed feed
Recycle (500%) or more.
6.4.2 clear bottom blowdown
After feeding artemia imago, clear bottom is primary daily, and blowdown is twice.
6.4.3 divide pond
Individual Size lag effects should carry out seed sorting, so far, Cynoglossus semilaevis to the survival rate of seed in time
Seed sorting, which still relies on, manually carries out range estimation sorting by seed size.When 2.5-3cm, breeding density is in 2000 tails/m2, 3.5-
When 4cm, breeding density is in 1000 tails/m2, when 5-8cm, breeding density is in 500 tails/m2。
Embodiment 2
A kind of method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population breeds high feminisation seed, comprising: parental source with choose
Choosing, daily management,
(1) parental source with select: selection bred primary 3-4 age Cynoglossus semilaevis parent population (female) 100 tails/pond,
Totally 1000 tail, to guarantee that kind of fish purity focuses on 2kg or so, each pond wherein purchasing wild 500 tail of Cynoglossus semilaevis parent population
Parent population weight is in 100kg.
(2) daily management: monitoring index includes the conventional water quality indicator such as temperature, salinity, dissolved oxygen, pH value, ammonia nitrogen, note
The diurnal variation and season situation of change and with being adjusted for recording each physical and chemical index, keep the stabilization of water quality, enclose sand using bidentate
Silkworm is fed.Water sand leaching water, water body are as clear as crystal；Water temperature: 23 DEG C；Salinity: 28-32 ‰；PH value: 7.8-8.6；Dissolution
Oxygen: 6mg/L or more；Ammonia nitrogen: less than 0.1mg/L；
(3) artificial insemination: the ovum extruded is mixed with sperm, after being gently mixed half a minute with hand, it is same that fish-egg is added
The water of weight continues to stir half a minute, and when stirring notices that hand cannot encounter chamber wall, and to stir evenly, static to use after twenty minutes
Clean seawater is cleaned, hatching；
(4) incubation step: static 20 minutes after fertilized eggs are cleaned, the fertilized eggs for choosing transparent floating are weighed, and are incubated
Change, generally using the 80 micro- Lotic hatchings of mesh net cage of placement, hatching density 500g/m in nursery pond3, hatch 23 DEG C of water temperature, salinity
30 ‰, illumination 500lux, silent as the grave ovum in each 2 removing net cages, micro- inflation sooner or later, carries out cloth pond after hatching 36h daily.
Although by reference to and combine preferred embodiment mode to the present invention have been described in detail, the present invention is not
It is limited to this.Without departing from the spirit and substance of the premise in the present invention, those of ordinary skill in the art can be to reality of the invention
Apply example and carry out various equivalent modifications or substitutions, and these modifications or substitutions all should in covering scope of the invention/it is any ripe
It knows those skilled in the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, should all cover
Within protection scope of the present invention.Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (7)
1. a kind of method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population breeds high feminisation seed characterized by comprising parent
It source and selects, parent fish rearing, artificial induced spawning and insemination and hatching, seed rearing:
(1) parental source with select: select 3-5 age Cynoglossus semilaevis parent population, raun weight is at 1500 grams or more, and milter is at 200 grams
More than, female-male proportion 1:5 further includes wild Cynoglossus semilaevis parent population to guarantee kind of a fish purity；
(2) parent fish rearing: monitoring index includes temperature, salinity, dissolved oxygen, pH value, ammonia nitrogen routine water quality indicator, records each reason
The diurnal variation and season situation of change and with being adjusted for changing index, keep the stabilization of water quality；
Breeding density raun controls in 1-3 tail/m2, milter can be controlled in 5-7 tail/m2；
Intensity of illumination is controlled in 500-800lux, and light is uniform, soft；Light application time shortens light since 18 hours daily
Stablize according to light application time after the time 5 minutes, 90 days in 11-12 hour；
It cultivates and uses water level sand leaching water, water body is as clear as crystal；Water temperature: 21-25 DEG C；Salinity: 28-32 ‰；PH value: 7.8-8.6；Dissolution
Oxygen: 6mg/L or more；Ammonia nitrogen: less than 0.1mg/L；
It is fed with fresh and alive perinereis aibihitensis Grube, and suitably feeds mixed feed, fed daily 2 times, each 1 time sooner or later, daily ration, feeding quantity
For the 1-3% of fish body weight, daily clear bottom 1 time, discharge water big blowdown 2 times, and quantity of exchanged water is no less than 8 circulations (800%) daily；
(3) artificial insemination: female parent fish selection is come out and carries out injection of hormone, hormone is luteotropin releasing hormone d-ala analog
(LRHL-A3) and the mixing of human chorionic gonadtropin (HCG)；
Intramuscular injection is used to female parent population with HCG/ kilograms of international unit of 5 microgram LRHL-A3+50 of dosage；
The ovum extruded is mixed with sperm, after being gently mixed half a minute with hand, the water that fish-egg equivalent weight is added continues to stir
Half a minute, when stirring, notice that hand cannot encounter chamber wall, and to stir evenly, static to be cleaned after twenty minutes with clean seawater,
Hatching；
Static 20 minutes after fertilized eggs are cleaned, the fertilized eggs for choosing transparent floating are weighed, and hatching generally uses nursery pond
The hatching of 80 mesh net cages of interior placement, hatching density 500g/m3,23 DEG C of water temperature of hatching, salinity 30 ‰, illumination 500lux are early daily
Silent as the grave ovum in evening each 2 removing net cages carries out cloth pond after hatching 36h；
(4) seed rearing: cloth ovum density be 10g/m3,23 DEG C of water temperature, salinity 30 ‰, pH8.0-8.4, dissolved oxygen 5mg/L or more,
Ammonia nitrogen is less than 0.1mg/L, intensity of illumination 2000lux；
Cloth pond after hatching 36h, the same day can hatch rupture of membranes, not need to feed within first 3 days, and the throwing of chlorella and wheel animalcule was carried out by the 4th day
Hello, the density that feeds of chlorella is 2,000,000/ml, and it is 5-8/ml that wheel animalcule, which feeds density, and the 10th day starts to feed artemia without section
The young, wheel animalcule are suitably reduced, and it is 0.5-1/ml that halogen children, which feeds density, and wheel animalcule stops throwing within the 15th day, halogen children increase feed 1-2/
Ml gradually started metamorphosis by the 18th day, and metamorphosis in the 24th day is basically completed, and fry is in the attached wall-like state in attached bottom, into the postlarva phase,
The feeding volume of halogen children continues to increase, and when 2.5-3cm gradually changes throwing artemia imago, and 3.5-4cm starts to tame mixed feed, wheel animalcule,
Halogen children needs to carry out fortification before feeding.
2. the method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population according to claim 1 breeds high feminisation seed, special
Sign is, in step (2), water temperature is 23 DEG C.
3. the method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population according to claim 1 breeds high feminisation seed, special
Sign is, in step (2), warm light regulation 2-4 months, preferably 3 months.
4. the method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population according to claim 1 breeds high feminisation seed, special
Sign is, in step (1), the accounting of wild Cynoglossus semilaevis parent population is 50%.
5. the method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population according to claim 1 breeds high feminisation seed, special
Sign is, in step (4), starts within the 6th day to change water, daily 10cm, to quantity of exchanged water when increasing when feeding halogen children, daily 1-2 are followed
Ring (100-200%), when feeding artemia imago, the daily 3-4 circulation (300-400%) of quantity of exchanged water, when feeding mixed feed, daily
It will be more than 5 circulations (500%).
6. the method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population according to claim 1 breeds high feminisation seed, special
Sign is, in step (4), after feeding artemia imago, clear bottom is primary daily, and blowdown is twice.
7. the method that nutrition regulation HUANGHE ESTUARY Cynoglossus semilaevis parent population according to claim 1 breeds high feminisation seed, special
Sign is, in step (4), when 2.5-3cm, breeding density is in 2000 tails/m2, when 3.5-4cm, breeding density is in 1000 tails/m2,
When 5-8cm, breeding density is in 500 tails/m2。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910482166.4A CN110178760B (en) | 2019-06-04 | 2019-06-04 | Method for breeding high-gynogenetic fry of parent fish of cynoglossus semilaevis in nutrition regulation and control manner |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910482166.4A CN110178760B (en) | 2019-06-04 | 2019-06-04 | Method for breeding high-gynogenetic fry of parent fish of cynoglossus semilaevis in nutrition regulation and control manner |
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| Publication Number | Publication Date |
|---|---|
| CN110178760A true CN110178760A (en) | 2019-08-30 |
| CN110178760B CN110178760B (en) | 2021-06-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111528146A (en) * | 2020-06-05 | 2020-08-14 | 中国水产科学研究院黄海水产研究所 | Method for obtaining high-female fertilized eggs of cynoglossus semilaevis all year round |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1644040A (en) * | 2005-01-13 | 2005-07-27 | 莱州明波水产有限公司 | Artificial breeding method for semi-smooth tongue sole |
| CN104522425A (en) * | 2014-12-30 | 2015-04-22 | 青岛农业大学 | Granular feed for adult fish of cynoglossus semilaevis and preparation method of granular feed |
| CN107287323A (en) * | 2017-07-14 | 2017-10-24 | 海阳市黄海水产有限公司 | The molecule selection of new varieties Cynoglossus semilaevis |
| CN107581105A (en) * | 2017-09-13 | 2018-01-16 | 唐山市维卓水产养殖有限公司 | A kind of method for improving Cynoglossus semilaevis raun seed ratio |
| CN108029902A (en) * | 2018-01-04 | 2018-05-15 | 广东越群海洋生物研究开发有限公司 | A kind of turning property of tongue sole feed and preparation method thereof |
-
2019
- 2019-06-04 CN CN201910482166.4A patent/CN110178760B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1644040A (en) * | 2005-01-13 | 2005-07-27 | 莱州明波水产有限公司 | Artificial breeding method for semi-smooth tongue sole |
| CN104522425A (en) * | 2014-12-30 | 2015-04-22 | 青岛农业大学 | Granular feed for adult fish of cynoglossus semilaevis and preparation method of granular feed |
| CN107287323A (en) * | 2017-07-14 | 2017-10-24 | 海阳市黄海水产有限公司 | The molecule selection of new varieties Cynoglossus semilaevis |
| CN107581105A (en) * | 2017-09-13 | 2018-01-16 | 唐山市维卓水产养殖有限公司 | A kind of method for improving Cynoglossus semilaevis raun seed ratio |
| CN108029902A (en) * | 2018-01-04 | 2018-05-15 | 广东越群海洋生物研究开发有限公司 | A kind of turning property of tongue sole feed and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 肖登元等: "维生素C对半滑舌鳎亲鱼繁殖性能及后代质量的影响", 《动物营养学报》 * |
| 陈松林等: "半滑舌鳎三倍体鱼苗的人工诱导与鉴定", 《水产学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111528146A (en) * | 2020-06-05 | 2020-08-14 | 中国水产科学研究院黄海水产研究所 | Method for obtaining high-female fertilized eggs of cynoglossus semilaevis all year round |
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