CN110167356A - Beer sample sparkling beverage - Google Patents
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- CN110167356A CN110167356A CN201780083072.9A CN201780083072A CN110167356A CN 110167356 A CN110167356 A CN 110167356A CN 201780083072 A CN201780083072 A CN 201780083072A CN 110167356 A CN110167356 A CN 110167356A
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- C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
- C12G3/06—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs with flavouring ingredients
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2250/00—Food ingredients
- A23V2250/24—Non-sugar sweeteners
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Abstract
Description
技术领域technical field
本发明涉及一种糖质含量被抑制且味道一致性良好、类啤酒性优异的啤酒样发泡性饮料。The present invention relates to a beer-like sparkling beverage with suppressed sugar content, good taste consistency, and excellent beer-like properties.
本申请基于2017年1月30日在日本申请的日本特愿2017-014011号主张优先权,并在此援用其内容。This application claims priority based on Japanese Patent Application No. 2017-014011 filed in Japan on January 30, 2017, and uses the content here.
背景技术Background technique
随着消费者嗜好的多样化,啤酒或发泡酒等啤酒样发泡性饮料正有多种多样的商品上市。尤其是根据近年来消费者的健康意向,对于低热量或低糖质的啤酒样发泡性饮料的需要提高。例如,啤酒虽已成为利用由酵母发酵生成的酒精以及尚未完全解析的多样成分而具有饮用口感且余味悠长的均衡性良好的止渴饮料,但热量或糖质含量较高。与此相对,不经由发酵工序而制造的非发酵啤酒样发泡性饮料具有比发酵啤酒样发泡性饮料的成分组成简单、饮用口感较弱的倾向,但容易控制热量或糖质含量。因此,例如,通过使用高甜度甜味料或水溶性食物纤维等更低糖质、低热量的原料代替蔗糖,能够比较容易地制造低糖质、低热量的非发酵啤酒样发泡性饮料。Along with the diversification of consumers' preferences, various products of beer-like sparkling beverages such as beer and malt liquor are being launched. In particular, the demand for low-calorie or low-sugar beer-like sparkling beverages has increased due to the health aspirations of consumers in recent years. For example, beer is a well-balanced thirst-quencher drink with a long aftertaste and a long aftertaste thanks to the alcohol produced by yeast fermentation and various components that have not yet been fully resolved, but it has high calorie and sugar content. On the other hand, non-fermented beer-like sparkling beverages produced without a fermentation process tend to have a simpler composition and weaker drinking texture than fermented beer-like sparkling beverages, but are easy to control calorie and sugar content. Therefore, for example, by using low-sugar and low-calorie raw materials such as high-intensity sweeteners or water-soluble dietary fibers instead of sucrose, low-sugar and low-calorie non-fermented beer-like sparkling beverages can be produced relatively easily.
高甜度甜味料有阿斯巴甜、蔗糖素、乙酰磺胺酸钾、糖精等人工甜味料、及甜菊提取物、甘草提取物、罗汉果提取物、索马甜、赤藻糖醇等天然甜味料。由于是更接近蔗糖的甜味物质,因此在饮料中广泛使用阿斯巴甜等人工甜味料。然而,大多高甜度甜味料的甜味回味感较强或具有苦味或涩味等,甜味物质与蔗糖不同。因此,已知有若干改善高甜度甜味料的甜味物质的方法。例如,在专利文献1中,记载了通过在含有乙酰磺胺酸钾及蔗糖素的饮料中添加酸味赋予物质及苦味赋予物质而能够改善乙酰磺胺酸钾等甜味的回味感。High-sweetness sweeteners include artificial sweeteners such as aspartame, sucralose, acesulfame potassium, and saccharin, and natural sweeteners such as stevia extract, licorice extract, monk fruit extract, thaumatin, and erythritol. sweetener. Since it is a sweetener closer to sucrose, artificial sweeteners such as aspartame are widely used in beverages. However, most high-intensity sweeteners have a strong sweet aftertaste, bitterness, astringency, etc., and sweet substances are different from sucrose. Accordingly, several methods for improving the sweetening substances of high-intensity sweeteners are known. For example, Patent Document 1 describes that the aftertaste of sweetness such as acesulfame potassium can be improved by adding a sour taste imparting substance and a bitter taste imparting substance to a beverage containing acesulfame potassium and sucralose.
添加至饮料中的水溶性食物纤维中有难消化性糊精、聚葡萄糖、大豆食物纤维等。另外,水溶性食物纤维也存在发挥除使饮料的提取物量增大而增强浓郁感以外的效果的情况。例如,在专利文献2中,记载了通过在包含钠盐的饮食物中添加水溶性食物纤维,而能够使咸味增强或掩盖因钾盐等产生的异味(刺激味)。另外,在专利文献3中,记载了通过在包含高甜度甜味料的碳酸饮料中配合难消化性糊精,而使泡稳定化。此外,尤其是难消化性糊精已知有抑制糖的吸收速度的作用或整肠作用等功能,因此可被优选地用于面向健康意向的消费者的饮料中。Among the water-soluble dietary fibers added to beverages are indigestible dextrin, polydextrose, soybean dietary fiber, and the like. In addition, water-soluble dietary fiber may also exhibit effects other than increasing the amount of the extract of the drink to enhance the body. For example, Patent Document 2 describes that by adding water-soluble dietary fiber to food and drink containing sodium salt, it is possible to enhance the salty taste or mask off-taste (stimulant taste) due to potassium salt or the like. In addition, Patent Document 3 describes that foam is stabilized by blending indigestible dextrin into a carbonated beverage containing a high-sweetness sweetener. In addition, especially indigestible dextrin is known to have functions such as an action of suppressing the absorption rate of sugar and an action of regulating the intestinal tract, so it can be preferably used in beverages for health-conscious consumers.
现有技术文献prior art literature
专利文献patent documents
专利文献1:日本特开2016-127855号公报Patent Document 1: Japanese Patent Laid-Open No. 2016-127855
专利文献2:国际公开第2012/017710号Patent Document 2: International Publication No. 2012/017710
专利文献3:日本特开2015-43765号公报Patent Document 3: Japanese Patent Laid-Open No. 2015-43765
发明内容Contents of the invention
发明要解决的问题The problem to be solved by the invention
本发明的目的在于提供一种糖质含量被抑制且味道一致性良好、类啤酒性优异的啤酒样发泡性饮料。An object of the present invention is to provide a beer-like sparkling beverage having a suppressed sugar content, good taste consistency, and excellent beer-like properties.
解决问题的技术手段technical means to solve problems
本发明者等人为了解决上述课题而进行了努力研究,结果发现,通过使用甜菊提取物或其酵素处理物作为高甜度甜味料且并用聚葡萄糖,可获得抑制糖质含量并且具备类啤酒性及良好的味道一致性的啤酒样发泡性饮料,从而完成了本发明。The inventors of the present invention conducted diligent research to solve the above-mentioned problems, and found that by using stevia extract or its enzyme-treated product as a high-intensity sweetener and using polydextrose in combination, it is possible to obtain a beer-like product with a suppressed sugar content. Beer-like sparkling beverages with high safety and good taste consistency, thereby completing the present invention.
本发明的啤酒样发泡性饮料是下述[1]至[5]。The beer-like sparkling beverage of the present invention is the following [1] to [5].
[1]一种啤酒样发泡性饮料,其特征在于含有10~80mg/L的甜菊醇糖苷、以及聚葡萄糖。[1] A beer-like sparkling drink characterized by containing 10 to 80 mg/L of steviol glycoside and polydextrose.
[2]如上述[1]的啤酒样发泡性饮料,其中上述聚葡萄糖的含量为4~40g/L。[2] The beer-like sparkling beverage according to the above [1], wherein the polydextrose content is 4 to 40 g/L.
[3]如上述[1]或[2]的啤酒样发泡性饮料,其为非发酵饮料。[3] The beer-like sparkling beverage according to [1] or [2] above, which is a non-fermented beverage.
[4]如上述[1]至[3]中任一项的啤酒样发泡性饮料,其中上述甜菊醇糖苷是从甜菊苷、甜菊双糖苷A、以及它们的糖基转移酶处理物所组成的组中所选的1种以上。[4] The beer-like sparkling beverage according to any one of the above-mentioned [1] to [3], wherein the above-mentioned steviol glycoside is composed of stevioside, steviolbioside A, and their glycosyltransferase-treated products 1 or more selected from the group.
[5]如上述[1]至[4]中任一项的啤酒样发泡性饮料,其中酒精含量小于1.0%。[5] The beer-like sparkling beverage as described in any one of [1] to [4] above, wherein the alcohol content is less than 1.0%.
发明的效果The effect of the invention
根据本发明,能够提供一种糖质含量被抑制且味道一致性良好、涩味与甜味的均衡性良好且类啤酒性优异的啤酒样发泡性饮料。According to the present invention, it is possible to provide a beer-like sparkling beverage having a suppressed sugar content, good taste uniformity, a good balance between astringency and sweetness, and excellent beer-like properties.
具体实施方式Detailed ways
本发明及本申请说明书中的所谓啤酒样发泡性饮料是指具有类啤酒性(香味上使人想起啤酒的味道)的发泡性饮料。本发明中的啤酒样发泡性饮料的酒精浓度并无限定,可为1.0体积%以上的酒精饮料,也可为小于1.0体积%的所谓无酒精饮料。另外,本发明中的啤酒样发泡性饮料可为经由利用酵母使发酵原料发酵的发酵工序而制造的发酵啤酒样发泡性饮料,也可为不经由发酵工序而制造的非发酵啤酒样发泡性饮料。具体而言,可列举啤酒、发泡酒、无酒精啤酒等。此外,也可为将经由发酵工序而制造的饮料与含酒精的蒸馏液混合而获得的甜露酒类。然而,就香味均衡性的观点而言,啤酒样发泡性饮料优选为非发酵的饮料。The so-called beer-like sparkling beverages in the present invention and the present specification refer to sparkling beverages having beer-like properties (taste reminiscent of beer in aroma). The alcohol concentration of the beer-like sparkling beverage in the present invention is not limited, and may be an alcoholic beverage of 1.0% by volume or more, or a so-called non-alcoholic beverage of less than 1.0% by volume. In addition, the beer-like sparkling beverage in the present invention may be a fermented beer-like sparkling beverage produced through a fermentation process of fermenting a fermentation material with yeast, or may be a non-fermented beer-like sparkling beverage produced without a fermentation process. Bubbly drinks. Specifically, beer, low-malt liquor, non-alcoholic beer, etc. are mentioned. In addition, liqueurs obtained by mixing a beverage produced through a fermentation process with an alcoholic distillate may also be used. However, the beer-like sparkling beverage is preferably a non-fermented beverage from the viewpoint of flavor balance.
本发明的啤酒样发泡性饮料含有甜菊醇糖苷作为甜味料。甜菊醇糖苷(具备甜菊醇骨架的糖苷)是来自甜菊(Stevia rebaudiana BERTONI)的叶的天然的高甜度甜味料,通过配合适量的甜菊醇糖苷,而能够与配合其它高甜度甜味料的情形同样地提升啤酒样发泡性饮料的浓郁感,改善饮用口感。The beer-like sparkling beverage of the present invention contains steviol glycoside as a sweetener. Steviol glycosides (glycosides with a steviol skeleton) are natural high-sweetness sweeteners derived from the leaves of Stevia rebaudiana BERTONI, and can be combined with other high-sweetness sweeteners by adding an appropriate amount of steviol glycosides. In the same way, the richness of the beer-like sparkling beverage can be improved, and the drinking taste can be improved.
甜菊醇糖苷具有特有的涩味,因此,一般在作为蔗糖的代替品添加至饮料时,大多情况下其涩味会成为饮料的异味。另外,甜菊醇糖苷的甜味余味较强,且甜味物质也与蔗糖不同,这一点对于作为蔗糖的代替品而言稍有问题。然而,在啤酒样发泡性饮料的情况下,该甜菊醇糖苷特有的涩味使主要来自啤酒花的类似啤酒的苦味及涩味提升。另外,源自甜菊醇糖苷的甜味是在与啤酒花苦味相同的时间被感受到,因此,因不自然的甜味损害类啤酒性的情况较少,反而通过味道的复杂性增加,而浓郁感提升,从而使饮用口感得到改善。如此,本发明的啤酒样发泡性饮料通过配合甜菊醇糖苷,而与使用蔗糖或其它高甜度甜味料的饮料相比,甜味与苦味的均衡性更良好,类啤酒性更优异。Steviol glycosides have a unique astringent taste, so when they are generally added to beverages as a substitute for sucrose, the astringent taste often becomes an off-flavor of the beverage. In addition, steviol glycosides have a strong aftertaste of sweetness and have a different sweetening substance from sucrose, which is a little problematic as a substitute for sucrose. However, in the case of beer-like sparkling beverages, the characteristic astringency of the steviol glycoside increases the beer-like bitterness and astringency mainly derived from hops. In addition, the sweetness derived from steviol glycosides is felt at the same time as the bitterness of hops, so the beer-like character is less likely to be impaired by unnatural sweetness, and the richness is enhanced by increasing the complexity of the taste. Enhanced, so that the drinking taste is improved. Thus, the beer-like sparkling beverage of the present invention has a better balance of sweetness and bitterness and better beer-like properties than beverages using sucrose or other high-sweetness sweeteners by incorporating steviol glycosides.
作为本发明的啤酒样发泡性饮料所含有的甜菊醇糖苷,只要为具有甜菊醇骨架的甜味成分则并无特别限定,可为甜菊的叶中含有的天然甜菊醇糖苷本身,也可为通过糖基转移酶对于天然的甜菊醇糖苷使葡萄糖或果糖进行糖基转移而成的甜菊醇糖苷。作为甜菊醇糖苷,例如可列举甜菊苷、甜菊双糖苷A、甜菊双糖苷C、及杜克苷A。本发明的啤酒样发泡性饮料所含有的甜菊醇糖苷可为1种,也可为2种以上。The steviol glycoside contained in the beer-like sparkling beverage of the present invention is not particularly limited as long as it is a sweet component having a steviol skeleton, and may be the natural steviol glycoside itself contained in the leaves of stevia, or Steviol glycoside obtained by transglycosylating glucose or fructose to natural steviol glycoside by glycosyltransferase. Examples of steviol glycosides include stevioside, steviolbioside A, steviolbioside C, and dulcoside A. The steviol glycoside contained in the beer-like sparkling beverage of the present invention may be one type, or two or more types.
含有甜菊醇糖苷的非发酵啤酒样发泡性饮料例如能够通过将含有甜菊醇糖苷的甜菊提取物、甜菊粉末、或由它们精制而得的甜菊醇糖苷作为原料而制造。甜菊提取物除利用适当的提取溶剂从甜菊的叶提取的提取物本身以外,也包含该提取物的粗精制物或生成物。另外,通过将对甜菊提取物、甜菊粉末、或由它们精制而得的甜菊醇糖苷进行糖基转移酶处理而成的物质(以下,有时称为“酵素处理甜菊”)作为原料,也能够制造本发明的啤酒样发泡性饮料。Non-fermented beer-like sparkling beverages containing steviol glycosides can be produced, for example, by using steviol glycoside-containing stevia extract, stevia powder, or steviol glycosides purified therefrom as raw materials. Stevia extract includes not only the extract itself extracted from the leaves of Stevia using a suitable extraction solvent, but also crudely purified or produced products of the extract. In addition, stevia extract, stevia powder, or steviol glycosides purified from them are treated with glycosyltransferase (hereinafter sometimes referred to as "enzyme-treated stevia") as raw materials, and it is also possible to produce The beer-like sparkling beverage of the present invention.
作为本发明的啤酒样发泡性饮料所含有的甜菊醇糖苷,优选为含有从甜菊苷、甜菊双糖苷A、以及它们的糖基转移酶处理物所组成的组中所选的1种以上,尤其是,更优选为含有从甜菊苷及其糖基转移酶处理物所组成的组中所选的1种以上,进一步优选为含有甜菊苷的糖基转移酶处理物。The steviol glycoside contained in the beer-like sparkling beverage of the present invention preferably contains one or more selected from the group consisting of stevioside, steviolbioside A, and their glycosyltransferase-treated products, In particular, it is more preferable that it contains one or more kinds selected from the group consisting of stevioside and its glycosyltransferase-treated product, and it is still more preferably a glycosyltransferase-treated product containing stevioside.
饮料中的甜菊醇糖苷的含量例如可根据通过HPLC(高速液相层析法)分析获得的色谱图的峰面积而求出。关于根据峰面积的定量方法,可根据面积百分率法求出,也可根据与浓度已知的标准品的峰面积的比而求出。The content of steviol glycosides in beverages can be determined, for example, from the peak area of a chromatogram obtained by HPLC (high performance liquid chromatography) analysis. As for the quantitative method based on the peak area, it can be obtained by the area percentage method, or can be obtained by the ratio of the peak area of a standard whose concentration is known.
作为本发明的啤酒样发泡性饮料的甜菊醇糖苷的含量,只要为能够赋予充分的饮用口感及类似啤酒的涩味且由甜菊醇糖苷产生的甜味不被感受为作为啤酒样发泡性饮料的异味的量,则并无特别限定,可考虑目标产品品质等而适当决定。作为本发明的啤酒样发泡性饮料的甜菊醇糖苷的含量,优选为10~80mg/L,更优选为20~80mg/L,进一步优选为20~50mg/L,进一步更优选为20~40mg/L。The content of steviol glycosides in the beer-like foaming beverage of the present invention is such that sufficient drinking texture and astringent taste similar to beer can be imparted and the sweetness produced by steviol glycosides is not perceived as a beer-like foaming beverage. The amount of the off-flavor of the beverage is not particularly limited, and may be appropriately determined in consideration of target product quality and the like. The content of steviol glycosides in the beer-like sparkling beverage of the present invention is preferably 10 to 80 mg/L, more preferably 20 to 80 mg/L, still more preferably 20 to 50 mg/L, and still more preferably 20 to 40 mg /L.
本发明的啤酒样发泡性饮料的甜菊醇糖苷的含量也可基于经蔗糖换算的含量来决定。例如,甜菊醇糖苷的含量优选为饮料的经蔗糖换算的含量成为1~15g/L的量,更优选为成为2.5~12g/L的量,进一步优选为成为3~7g/L的量。The content of the steviol glycoside in the beer-like sparkling beverage of the present invention can also be determined based on the content in terms of sucrose. For example, the content of steviol glycosides is preferably 1 to 15 g/L, more preferably 2.5 to 12 g/L, and even more preferably 3 to 7 g/L in terms of sucrose content of the beverage.
另外,所谓“高甜度甜味料的经蔗糖换算的含量”是指使高甜度甜味料的含量乘以甜度而得的量。例如,在甜度180的酵素处理甜菊为28mg/L的情况下,经蔗糖换算的量成为5g/L。In addition, the "content in terms of sucrose conversion of the high-intensity sweetener" means the amount obtained by multiplying the content of the high-intensity sweetener by the degree of sweetness. For example, when the enzyme-treated stevia with a sweetness of 180 is 28 mg/L, the amount in terms of sucrose is 5 g/L.
本发明的啤酒样发泡性饮料除甜菊醇糖苷以外,也含有聚葡萄糖作为水溶性食物纤维。通过尤其使用聚葡萄糖作为水溶性食物纤维,而因甜菊醇糖苷产生的涩味或甜味一致,能够进一步改善类似啤酒的良好的涩味及浓郁感。The beer-like sparkling beverage of the present invention contains polydextrose as a water-soluble dietary fiber in addition to steviol glycosides. In particular, by using polydextrose as the water-soluble dietary fiber, the astringency and sweetness due to steviol glycosides can be uniformed, and the beer-like good astringency and richness can be further improved.
作为本发明的啤酒样发泡性饮料的聚葡萄糖的含量,只要为能够将由甜菊醇糖苷产生的甜味及涩味整体改善为更温和的味道的量,则并无特别限定,可考虑所配合的甜菊醇糖苷的量或目标产品品质等而适当决定。作为本发明的啤酒样发泡性饮料的聚葡萄糖的含量,优选为4~40g/L,更优选为4~30g/L,进一步优选为4.5~25g/L,进一步更优选为6~20g/L。The content of polydextrose in the beer-like sparkling beverage of the present invention is not particularly limited as long as it can improve the overall sweetness and astringency produced by steviol glycosides to a milder taste. The amount of steviol glycosides or the quality of the target product is appropriately determined. The content of polydextrose in the beer-like sparkling beverage of the present invention is preferably 4 to 40 g/L, more preferably 4 to 30 g/L, still more preferably 4.5 to 25 g/L, and even more preferably 6 to 20 g/L. L.
本发明的啤酒样发泡性饮料通过使用甜菊醇糖苷作为甜味料,即便不使用蔗糖,也能够达成类似啤酒的饮用口感。作为本发明的啤酒样发泡性饮料的糖质浓度,优选为小于2.0g/100mL,更优选为小于0.9g/100mL,进一步优选为小于0.5g/100mL。本发明的啤酒样发泡性饮料的糖质浓度例如可通过仅使用糖质含量相对较低者作为原料而制造。In the beer-like sparkling beverage of the present invention, by using steviol glycoside as a sweetener, it is possible to achieve a beer-like mouthfeel without using sucrose. The sugar concentration of the beer-like sparkling beverage of the present invention is preferably less than 2.0 g/100 mL, more preferably less than 0.9 g/100 mL, even more preferably less than 0.5 g/100 mL. The sugar concentration of the beer-like sparkling beverage of the present invention can be produced, for example, by using only those with a relatively low sugar content as raw materials.
作为本发明的啤酒样发泡性饮料,由于顺应消费者的健康意向,因此优选嘌呤体浓度较低。本发明的啤酒样发泡性饮料的嘌呤体浓度优选为小于0.2mg/100mL,更优选为0.1mg/100mL以下,进一步优选为小于0.08mg/100mL。As the beer-like sparkling beverage of the present invention, it is preferable that the concentration of purine body is low because it complies with consumers' health intentions. The purine body concentration of the beer-like sparkling beverage of the present invention is preferably less than 0.2 mg/100 mL, more preferably 0.1 mg/100 mL or less, even more preferably less than 0.08 mg/100 mL.
另外,在本发明及本申请说明书中,所谓嘌呤体,是指腺嘌呤、黄嘌呤、鸟嘌呤、次黄嘌呤的嘌呤体碱基4种的总量。啤酒样发泡性饮料或原料中的嘌呤体含量例如可通过在利用高氯酸进行的水解后使用LC-MS/MS进行检测的方法(“酒类的嘌呤体的微量分析的指导”,财团法人日本食品分析中心,因特网<URL:http://www.jfrl.or.jp/item/nutrition/post-31.html>,2013年1月检索)进行测定。In addition, in the present invention and the description of the present application, the purine body refers to the total amount of four kinds of purine body bases of adenine, xanthine, guanine, and hypoxanthine. The content of purine bodies in beer-like sparkling beverages or raw materials can be detected, for example, by a method using LC-MS/MS after hydrolysis with perchloric acid ("Guidelines for microanalysis of purine bodies in alcoholic beverages", Consortium Japan Food Analysis Center, Internet <URL: http://www.jfrl.or.jp/item/nutrition/post-31.html>, searched in January 2013) for measurement.
本发明的啤酒样发泡性饮料优选为仅含有甜菊醇糖苷作为甜味料的饮料,但在无损本发明效果的范围内,也可含有甜菊醇糖苷以外的甜味料。作为甜菊醇糖苷以外的甜味料,可为砂糖,可为甜度相对较低者,也可为高甜度甜味料。作为甜度相对较低的甜味料,具体而言,可列举多糖类、甜味系胺基酸。所谓多糖类,是指3个以上的单糖聚合而成的糖质。多糖类主要根据其大小大致分为淀粉、糊精及低聚糖。低聚糖是3~10个左右的单糖聚合而成的糖质,糊精是将淀粉水解而获得的糖质,且比低聚糖大。作为甜味系胺基酸,可列举丙胺酸或甘胺酸,优选为丙胺酸。作为高甜度甜味料,可列举乙酰磺胺酸钾、纽甜、阿斯巴甜、蔗糖素等。这些甜味料可仅使用1种,也可将2种以上并用。The beer-like sparkling beverage of the present invention preferably contains only steviol glycoside as a sweetener, but may contain sweeteners other than steviol glycoside within the range that does not impair the effect of the present invention. As sweeteners other than steviol glycosides, sugar may be used, and sweeteners with relatively low sweetness may be used, or high-sweetness sweeteners may be used. Specific examples of sweeteners with relatively low sweetness include polysaccharides and sweetening amino acids. The so-called polysaccharides refer to sugars in which three or more monosaccharides are aggregated. Polysaccharides are roughly divided into starches, dextrins, and oligosaccharides based on their size. Oligosaccharides are saccharides formed by polymerization of about 3 to 10 monosaccharides, and dextrins are saccharides obtained by hydrolyzing starch, and are larger than oligosaccharides. Examples of sweet amino acids include alanine and glycine, preferably alanine. Examples of high-intensity sweeteners include acesulfame potassium, neotame, aspartame, sucralose, and the like. These sweeteners may use only 1 type, and may use 2 or more types together.
本发明的啤酒样发泡性饮料可为仅含有聚葡萄糖作为水溶性食物纤维的饮料,也可为含有聚葡萄糖以外的水溶性食物纤维的饮料。所谓水溶性食物纤维,是指溶解在水中且不被或难以被人的消化酶消化的碳水化合物。通过含有水溶性食物纤维,成为饮用口感更良好的啤酒样发泡性饮料。作为配合至本发明的啤酒样发泡性饮料中的聚葡萄糖以外的水溶性食物纤维,例如可列举难消化性糊精、大豆食物纤维、半乳甘露聚糖、菊糖、瓜尔胶分解物、果胶、阿拉伯胶等。这些水溶性食物纤维可仅使用1种,也可将2种以上并用。The beer-like sparkling beverage of the present invention may contain only polydextrose as a water-soluble dietary fiber, or may contain a water-soluble dietary fiber other than polydextrose. The so-called water-soluble dietary fiber refers to carbohydrates that dissolve in water and are not or difficult to be digested by human digestive enzymes. By containing water-soluble dietary fiber, it becomes a beer-like sparkling drink with a better drinking texture. Examples of water-soluble dietary fibers other than polydextrose blended in the beer-like foaming beverage of the present invention include indigestible dextrin, soybean dietary fiber, galactomannan, inulin, and guar gum decomposition products. , pectin, gum arabic, etc. These water-soluble dietary fibers may use only 1 type, and may use 2 or more types together.
本发明的啤酒样发泡性饮料优选为进一步含有酸味料。通过含有酸味料,可获得香味的均衡性优异、类啤酒性更强的啤酒样发泡性饮料。作为酸味料,只要为能够配合至饮食品中的酸味料,则并无特别限定,可根据对最终产品要求的品质特性,与其配合量一起适当决定。作为酸味料,只要通常用在饮食品的制造中,则并无特别限定,例如可列举乳酸、柠檬酸、葡萄糖酸、苹果酸、酒石酸、富马酸、琥珀酸、己二酸、富马酸、磷酸、及它们的盐等。这些酸味料可仅使用1种,也可将2种以上并用而使用。It is preferable that the beer-like sparkling beverage of the present invention further contains a sour agent. By containing an acidulant, a beer-like sparkling beverage having excellent flavor balance and stronger beer-like properties can be obtained. The acidulant is not particularly limited as long as it can be incorporated into food and beverages, and can be appropriately determined together with the amount of the acidulant according to the quality characteristics required for the final product. The sour agent is not particularly limited as long as it is generally used in the manufacture of food and drink, for example, lactic acid, citric acid, gluconic acid, malic acid, tartaric acid, fumaric acid, succinic acid, adipic acid, fumaric acid , phosphoric acid, and their salts. These acidulant may use only 1 type, and may use it in combination of 2 or more types.
在本发明的啤酒样发泡性饮料含有酸的情况下,优选为以饮料的pH值成为3.0~4.0的方式调整酸的添加量。若添加至饮料中的酸的量过多,则会导致酸味加强,而变得难喝。通过以饮料的pH值成为3.0~4.0左右的方式调节酸的量进行添加,能够在制造的非发酵啤酒样发泡性饮料中,使香味的均衡性进一步改善。When the beer-like sparkling beverage of the present invention contains an acid, it is preferable to adjust the addition amount of the acid so that the pH of the beverage becomes 3.0 to 4.0. If too much acid is added to the drink, the sour taste will be enhanced and it will become unpalatable. By adjusting the amount of the acid and adding the acid so that the pH of the drink becomes about 3.0 to 4.0, the flavor balance can be further improved in the produced non-fermented beer-like sparkling drink.
本发明的啤酒样发泡性饮料优选为进一步含有苦味料。苦味是啤酒的特征之一,通过含有苦味料,可获得类啤酒性更强的啤酒样发泡性饮料。作为苦味料,只要在作为产品的啤酒样发泡性饮料中呈现出与啤酒同质或者近似的苦味,则并无特别限定,可为啤酒花中包含的苦味成分,也可为啤酒花中不含的苦味成分。作为该苦味料,具体而言,可代表性地列举:镁盐、钙盐、柠檬酸三丁酯、柠檬酸三乙酯、柚苷、苦木素、异α酸、四异α酸、β酸的氧化物、奎宁、苦瓜蛋白、槲皮苷、可可碱、咖啡因等苦味赋予成分、及苦瓜、当药茶、苦丁茶、洋艾提取物、龙胆草提取物、金鸡纳提取物等苦味赋予素材。这些苦味料可仅使用1种,也可将2种以上并用。It is preferable that the beer-like sparkling beverage of the present invention further contains a bittering agent. Bitterness is one of the characteristics of beer, and by including a bittering agent, a beer-like sparkling beverage having a stronger beer-like property can be obtained. The bittering agent is not particularly limited as long as it exhibits the same or similar bitterness to beer in the beer-like sparkling beverage as the product, and it may be a bittering component contained in hops, or may be a bittering component not contained in hops. Bitter ingredients. Specific examples of the bittering agent include magnesium salts, calcium salts, tributyl citrate, triethyl citrate, naringin, picrin, iso-α acid, tetraiso-α acid, β Acid oxides, quinine, bitter melon protein, quercetin, theobromine, caffeine and other bitter ingredients, and bitter melon, herbal tea, Kudingcha, wormwood extract, gentian extract, cinchona extract Gives materials such as bitterness. These bittering agents may use only 1 type, and may use 2 or more types together.
大多数啤酒为浅黄色~深褐色或黑色的透明溶液。通过使本发明的啤酒样发泡性饮料进一步含有色素,可赋予与啤酒相同的颜色。作为该色素,只要为可赋予类似啤酒的颜色且可食用的色素,则并无特别限定,特别优选为焦糖化反应物(焦糖色素)。Most beers are light yellow to dark brown or black transparent solutions. The beer-like sparkling beverage of the present invention can be given the same color as beer by further containing a pigment. The coloring matter is not particularly limited as long as it can impart a beer-like color and is edible, but a caramelization reaction product (caramel coloring) is particularly preferable.
在本发明的啤酒样发泡性饮料含有色素的情况下,该色素的含量可根据所使用的色素的种类或对最终产品要求的品质特性等进行适当调整。作为本发明的啤酒样发泡性饮料,优选为以饮料的色度成为2°EBC以上的方式添加色素,更优选为以成为5°EBC以上的方式进行添加,进一步优选为以成为7°EBC以上的方式进行添加,进一步更优选为以成为7~16°EBC的方式进行添加。另外,色度可通过EBC(European Brewery Convention)的Analytica-EBC标准法或依据其的方法进行测定。EBC是啤酒的分析中的色度单位,由数值(利用具有EBC色度的9个玻璃圆盘的比色器,以目视进行测定,或者基于波长430nm下的吸光度算出)表示啤酒颜色的深浅。When the beer-like sparkling beverage of the present invention contains a colorant, the content of the colorant can be appropriately adjusted according to the type of colorant to be used, quality characteristics required for the final product, and the like. As the beer-like sparkling beverage of the present invention, the coloring is preferably added so that the chroma of the beverage becomes 2° EBC or higher, more preferably 5° EBC or higher, even more preferably 7° EBC It is added as above, and it is more preferable to add so that it may become 7-16 degree EBC. In addition, chromaticity can be measured by the Analytica-EBC standard method of EBC (European Brewery Convention) or the method based on it. EBC is a chromaticity unit in the analysis of beer, and the depth of beer color is expressed by numerical value (measured visually using a colorimeter with 9 glass discs with EBC chromaticity, or calculated based on absorbance at a wavelength of 430nm) .
啤酒的外观上的最大特征是在注入至容器时泛起白色的漂亮的泡沫。因此,本发明的啤酒样发泡性饮料优选为进一步含有起泡剂。作为该起泡剂,例如可列举大豆食物纤维、大豆肽、大豆皂苷、海藻酸酯、皂树皂苷等。这些起泡剂可仅使用1种,也可将2种以上并用而使用。作为本发明的啤酒样发泡性饮料,优选为含有从大豆肽、大豆食物纤维、海藻酸酯、及皂树皂苷所组成的组中所选的1种以上,更优选为含有从大豆食物纤维及皂树皂苷所组成的组中所选的1种以上。The biggest characteristic of the appearance of beer is beautiful white foam when it is poured into a container. Therefore, it is preferable that the beer-like sparkling beverage of the present invention further contains a foaming agent. Examples of the foaming agent include soybean dietary fiber, soybean peptide, soybean saponin, alginate, saponin, and the like. These blowing agents may be used alone or in combination of two or more. The beer-like sparkling beverage of the present invention preferably contains one or more selected from the group consisting of soybean peptide, soybean dietary fiber, alginate, and saponins, and more preferably contains soybean dietary fiber and one or more selected from the group consisting of Quillaja saponins.
在本发明的啤酒样发泡性饮料含有起泡剂的情况下,该起泡剂的含量可根据所使用的起泡剂的种类或组合、对最终产品要求的品质特性等进行适当调整。作为本发明的啤酒样发泡性饮料,优选为以饮料的NIBEM值成为80以上的方式添加起泡剂,更优选为以成为100以上的方式进行添加,进一步优选为以成为150以上的方式进行添加。另外,NIBEM值是以导电率测定所注入的泡沫的崩解速度而得到的,通常用于啤酒等的泡沫持久性评价中。非发酵啤酒样发泡性饮料的NIBEM值可通过EBC(European Brewery Convention)的Analytica-EBC标准法或依据其的方法进行测定。When the beer-like sparkling beverage of the present invention contains a foaming agent, the content of the foaming agent can be appropriately adjusted according to the type or combination of foaming agents used, quality characteristics required for the final product, and the like. In the beer-like sparkling beverage of the present invention, the foaming agent is preferably added so that the NIBEM value of the beverage becomes 80 or higher, more preferably 100 or higher, and still more preferably 150 or higher. Add to. In addition, the NIBEM value is obtained by measuring the disintegration rate of the injected foam by electrical conductivity, and is generally used in the evaluation of the foam durability of beer and the like. The NIBEM value of the non-fermented beer-like sparkling beverage can be measured by the Analytica-EBC standard method of EBC (European Brewery Convention) or a method based thereon.
作为本发明的啤酒样发泡性饮料,可为含有乙醇的酒精饮料,也可为不含乙醇的无酒精饮料。在本发明的啤酒样发泡性饮料含有乙醇的情况下,饮料中的乙醇浓度优选为1~9体积%,更优选为1~8体积%,进一步优选为1~7体积%。另外,含有乙醇的非发酵啤酒样发泡性饮料可通过将含酒精的蒸馏液作为原料而进行制造。含酒精的蒸馏液是指通过蒸馏操作而获得的含有酒精的溶液,可使用通常被分类为蒸馏酒者。例如可使用在酒类的制造中通常使用的原料用酒精、烈性酒、威士忌、白兰地、伏特加、朗姆酒、龙舌兰酒、杜松酒、烧酒等。The beer-like sparkling beverage of the present invention may be an alcoholic beverage containing ethanol or a non-alcoholic beverage not containing ethanol. When the beer-like sparkling beverage of the present invention contains ethanol, the ethanol concentration in the beverage is preferably 1 to 9 vol%, more preferably 1 to 8 vol%, and still more preferably 1 to 7 vol%. In addition, non-fermented beer-like sparkling beverages containing ethanol can be produced by using alcohol-containing distillates as raw materials. Alcoholic distillate refers to a solution containing alcohol obtained through distillation operations, and those generally classified as distilled spirits can be used. For example, raw material alcohol, spirits, whiskey, brandy, vodka, rum, tequila, gin, shochu, etc. which are generally used in the production of liquor can be used.
本发明的啤酒样发泡性饮料可在无损本发明的效果的范围内进一步含有其它成分。作为该其它成分,可列举来自酵母或来自小麦等谷物的蛋白质分解物、麦芽糖化液、果汁、香草、香料等。The beer-like sparkling beverage of the present invention may further contain other components within the range not impairing the effects of the present invention. Examples of such other components include protein breakdown products derived from yeast or grains such as wheat, maltosaccharified liquid, fruit juice, herbs, spices, and the like.
作为香料,例如可列举啤酒风味料、啤酒香料、啤酒花香料、麦芽香料、碳酸增强剂等。Examples of flavors include beer flavorings, beer flavors, hop flavors, malt flavors, carbonation enhancers and the like.
本发明的啤酒样发泡性饮料除将甜菊醇糖苷及聚葡萄糖作为原料以外,可以与一般的啤酒样发泡性饮料的制造相同的方式进行制造。The beer-like sparkling beverage of the present invention can be produced in the same manner as the production of general beer-like sparkling beverages, except that steviol glycoside and polydextrose are used as raw materials.
在本发明的啤酒样发泡性饮料为非发酵啤酒样发泡性饮料的情况下,例如可通过如下制造方法进行制造,该制造方法具有:将包含甜菊醇糖苷的原料、聚葡萄糖及其它原料混合而调制调合液的调制工序、及在通过上述调制工序所获得的调合液中添加二氧化碳的气体导入工序或添加碳酸水的加水工序。在使用啤酒花或啤酒花提取物等的包含α酸的原料作为苦味料的情况下,可通过如下制造方法进行制造,该制造方法具有:将原料混合而调制调合液的调制工序、对通过上述调制工序而获得的调合液进行煮沸处理而使α酸异构化的异构化工序、及在上述异构化工序后向异构化的调合液中添加二氧化碳的气体导入工序或添加碳酸水的加水工序。煮沸方法及其条件可适当决定。另外,香料或色素的混合时机可与其它原料同时,也可在气体导入工序或加水工序之后。When the beer-like sparkling beverage of the present invention is a non-fermented beer-like sparkling beverage, it can be produced, for example, by a production method comprising: mixing a raw material containing steviol glycosides, polydextrose, and other raw materials A preparation step of mixing to prepare a liquid preparation, a gas introduction step of adding carbon dioxide to the liquid preparation obtained in the above preparation step, or a water addition step of adding carbonated water. In the case of using a raw material containing alpha acid such as hops or hop extract as a bittering material, it can be produced by a production method including a preparation step of mixing the raw materials to prepare a prepared liquid, The isomerization step of boiling the prepared solution obtained in the step to isomerize the α acid, and the gas introduction step of adding carbon dioxide or adding carbonated water to the isomerized prepared solution after the above-mentioned isomerization step of water addition process. The boiling method and its conditions can be appropriately determined. In addition, the mixing timing of the fragrance or coloring matter may be at the same time as other raw materials, or after the gas introduction step or the water addition step.
首先,在调制工序中,通过将原料混合,而调制调合液。在调制工序中,优选为将二氧化碳、香料及色素以外的全部原料混合而调制调合液。将各原料混合顺序并无特别限定。可在原料水中同时添加全部原料,也可在使先添加的原料溶解之后再添加其余的原料等依次添加原料。另外,例如可将固体(例如,粉末状或颗粒状)原料及酒精混合在原料水中,也可预先将固体原料制成水溶液,再将这些水溶液及酒精、视需要的原料水进行混合。First, in the preparation step, raw materials are mixed to prepare a prepared liquid. In the preparation step, it is preferable to prepare a prepared liquid by mixing all raw materials other than carbon dioxide, fragrance and coloring matter. The order of mixing the raw materials is not particularly limited. All the raw materials may be added simultaneously to the raw material water, or the raw materials may be added sequentially after dissolving the first added raw materials and then adding the remaining raw materials. In addition, for example, a solid (for example, powdery or granular) raw material and alcohol may be mixed in raw water, or the solid raw material may be made into an aqueous solution in advance, and then the aqueous solution, alcohol, and optionally raw water may be mixed.
在调制工序中调制的调合液中产生不溶物的情况下,优选为在任一工序中对该调合液进行过滤等将不溶物去除的处理。尤其是,在进行异构化工序的情况下,优选为从异构化处理后的调合液去除不溶物。不溶物去除处理并无特别限定,可通过过滤法、离心分离法等在该技术领域中通常使用的方法进行。在本发明中,不溶物优选为过滤去除,更优选为利用硅藻土过滤进行去除。When insoluble matter is generated in the prepared liquid prepared in the preparation step, it is preferable to perform a treatment for removing the insoluble matter such as filtering the prepared liquid in either step. In particular, when performing the isomerization step, it is preferable to remove insoluble matter from the prepared liquid after the isomerization treatment. The insoluble matter removal treatment is not particularly limited, and can be performed by a method generally used in this technical field, such as a filtration method and a centrifugation method. In the present invention, the insoluble matter is preferably removed by filtration, more preferably by diatomaceous earth filtration.
接着,作为气体导入工序,在通过调制工序获得的调合液中添加二氧化碳。由此,获得非发酵啤酒样发泡性饮料。通过添加碳酸,赋予与啤酒相同的爽快感。另外,二氧化碳的添加可通过常规方法进行。例如,可将通过调制工序获得的调合液与碳酸水混合,也可在通过调制工序获得的调合液中直接添加二氧化碳并使其溶解。Next, as a gas introduction step, carbon dioxide is added to the prepared liquid obtained in the preparation step. Thereby, a non-fermented beer-like sparkling beverage was obtained. By adding carbonation, it gives the same refreshment as beer. In addition, addition of carbon dioxide can be performed by a conventional method. For example, the prepared solution obtained in the preparation step may be mixed with carbonated water, or carbon dioxide may be directly added and dissolved in the prepared solution obtained in the preparation step.
添加二氧化碳之后,可对所获得的非发酵啤酒样发泡性饮料进一步进行过滤等将不溶物去除的处理。不溶物去除处理并无特别限定,可通过在该技术领域中通常使用的方法而进行。After adding carbon dioxide, the obtained non-fermented beer-like sparkling beverage may be further subjected to a treatment such as filtration to remove insoluble matter. The insoluble matter removal treatment is not particularly limited, and can be performed by a method generally used in this technical field.
所获得的非发酵啤酒样发泡性饮料通常通过填充工序而填充至罐、瓶、PET瓶等容器中而作为产品出厂。The obtained non-fermented beer-like sparkling beverage is usually filled into containers such as cans, bottles, and PET bottles through a filling process, and shipped as a product.
在本发明的啤酒样发泡性饮料为发酵啤酒样发泡性饮料的情况下,例如可通过酿造(发酵原料液调制)、发酵、贮酒、过滤的工序进行制造。作为原料的包含甜菊醇糖苷的原料及聚葡萄糖的添加时间或添加量是以在最终获得的饮料中的浓度包含在所期望浓度的范围内的方式进行适当调整。包含甜菊醇糖苷的原料及聚葡萄糖例如可在酿造工序中与发酵原料等同时进行添加,也可添加至发酵工序后的发酵液中。When the beer-like sparkling beverage of the present invention is a fermented beer-like sparkling beverage, it can be produced, for example, through the steps of brewing (preparation of fermentation raw material liquid), fermentation, wine storage, and filtration. The addition time and amount of the raw material containing steviol glycoside and polydextrose as raw materials are appropriately adjusted so that the concentration in the finally obtained beverage falls within the range of the desired concentration. The raw material and polydextrose containing steviol glycoside may be added simultaneously with fermentation raw material etc. in a brewing process, for example, and may be added to the fermentation liquid after a fermentation process.
首先,作为酿造工序(发酵原料液调制工序),对包含发酵原料及水的混合物进行糖化处理,对所获得的糖化液进行煮沸处理。具体而言,首先,调制包含发酵原料及原料水的混合物并进行加温,使发酵原料的淀粉质糖化。作为发酵原料,可仅使用谷物原料,也可仅使用糖质原料,也可将两者混合而使用。First, as a brewing step (fermentation raw material liquid preparation step), a mixture containing a fermentation raw material and water is subjected to a saccharification treatment, and the obtained saccharification liquid is subjected to a boiling treatment. Specifically, first, a mixture containing fermentation raw materials and raw material water is prepared and heated to saccharify the starch of the fermentation raw materials. As a fermentation raw material, only a grain raw material may be used, only a sugary raw material may be used, and both may be mixed and used.
作为谷物原料,例如可列举大麦或小麦、它们的麦芽等麦类、米、玉米、大豆等豆类、薯类等。谷物原料可以谷物糖浆、谷物提取物等的形式使用,优选为以进行粉碎处理而获得的谷物粉碎物的形式使用。谷物类的粉碎处理可通过常规方法进行。作为谷物粉碎物,也可为如麦芽粉碎物、玉米淀粉、玉米粗粉等那样实施在粉碎处理的前后通常进行的处理。麦芽粉碎物只要为通过常规方法使大麦、例如二棱大麦发芽并将其干燥后粉碎成特定的粒度即可。作为在本发明中所使用的谷物原料,可为1种谷物原料,也可为将复数种谷物原料混合而成的原料。例如,可使用麦芽粉碎物作为主原料,并使用米或玉米的粉碎物作为副原料。作为糖质原料,例如可列举液糖等糖类。Examples of grain raw materials include barley, wheat, and barley such as malt thereof, beans such as rice, corn, and soybeans, potatoes, and the like. The grain raw material can be used in the form of grain syrup, grain extract, etc., and is preferably used in the form of ground grain obtained by grinding. The pulverization process of cereals can be performed by a conventional method. The ground grains may be subjected to the usual treatment before and after the grinding treatment, such as malt ground material, cornstarch, corn meal, and the like. The ground malt product may be ground to a specific particle size after germinating and drying barley, for example, two-row barley by a conventional method. As the grain raw material used in this invention, one kind of grain raw material may be sufficient as it, and the raw material which mixed several types of grain raw materials may be sufficient. For example, ground malt can be used as a main raw material, and ground rice or corn can be used as an auxiliary raw material. As a carbohydrate raw material, sugars, such as liquid sugar, are mentioned, for example.
糖化处理是利用来自谷物原料等的酵素或另外添加的酵素而进行。糖化处理时的温度或时间可考虑所使用的谷物原料等的种类、谷物原料在发酵原料整体中所占的比率、所添加的酵素的种类或混合物的量、目标发酵啤酒样发泡性饮料的品质等而适当调整。例如,糖化处理可通过将包含谷物原料等的混合物在35~70℃下保持20~90分钟等常规方法而进行。另外,优选为添加α-淀粉酶、葡萄糖淀粉酶、普鲁兰酶等糖化酶或蛋白酶等酵素剂。The saccharification treatment is performed using enzymes derived from grain raw materials or other enzymes added separately. The temperature or time during the saccharification process may take into account the type of grain material used, the ratio of the grain material to the entire fermentation material, the type of enzyme to be added or the amount of the mixture, and the desired fermented beer-like sparkling beverage. quality and so on. For example, the saccharification process can be performed by a common method, such as holding the mixture containing a grain raw material etc. at 35-70 degreeC for 20-90 minutes. In addition, it is preferable to add saccharifying enzymes such as α-amylase, glucoamylase, and pullulanase, or enzyme agents such as protease.
通过将在糖化处理后获得的糖化液煮沸,可调制汤汁(糖化液的煮沸物)。糖化液优选为在煮沸处理前进行过滤,对所获得的滤液进行煮沸处理。另外,也可使用在麦芽提取物中添加温水而成者代替该糖化液的滤液,并将其煮沸。煮沸方法及其条件可适当决定。Soup (boiled product of saccharification liquid) can be prepared by boiling the saccharification liquid obtained after the saccharification process. The saccharified liquid is preferably filtered before the boiling treatment, and the obtained filtrate is subjected to the boiling treatment. Moreover, what added warm water to the malt extract can also be used instead of the filtrate of this saccharified liquid, and it can boil it. The boiling method and its conditions can be appropriately determined.
通过在煮沸处理前或煮沸处理中适当添加香草等,能够制造具有所期望香味的发酵啤酒样发泡性饮料。尤其是啤酒花优选为在煮沸处理前或煮沸处理中进行添加。通过在啤酒花的存在下进行煮沸处理,能够高效地煮出啤酒花的风味、香气成分。啤酒花的添加量、添加方式(例如分数次进行添加等)及煮沸条件可适当决定。A fermented beer-like sparkling drink having a desired flavor can be produced by appropriately adding herbs or the like before or during the boiling treatment. In particular, hops are preferably added before or during the boiling treatment. By performing the boiling treatment in the presence of hops, flavor and aroma components of hops can be efficiently boiled out. The amount of hops to be added, the method of addition (for example, addition in fractional times, etc.), and boiling conditions can be appropriately determined.
在通过酿造工序获得的汤汁(煮沸过的糖化液)中包含因沉淀而产生的蛋白质等糟粕。因此,优选为在发酵工序前,作为固液分离处理工序,将至少一部分的固形物成分从该汤汁中去除。将固形物成分去除的固液分离处理一般是通过使用被称为漩涡槽的槽将沉淀物去除而进行。此时的汤汁的温度只要为15℃以上即可,一般在50~100℃左右进行。将糟粕去除后的汤汁(滤液)是利用板式冷却器等冷却至适当的发酵温度。可将去除该糟粕之后的汤汁直接作为发酵原料液,也可以将去除后的汤汁调整为所期望提取物浓度而得到的液体作为发酵原料液。The broth (boiled saccharified liquid) obtained through the brewing process contains dregs such as protein produced by precipitation. Therefore, it is preferable to remove at least a part of solid content from the broth as a solid-liquid separation treatment step before the fermentation step. The solid-liquid separation process for removing solid components is generally performed by removing sediment using a tank called a whirl tank. The temperature of the soup stock at this time should just be 15 degreeC or more, and it generally carries out at about 50-100 degreeC. The broth (filtrate) from which dregs have been removed is cooled to an appropriate fermentation temperature using a plate cooler or the like. The broth after removing the dross may be directly used as a fermentation raw material liquid, or the liquid obtained by adjusting the removed broth to a desired extract concentration may be used as a fermentation raw material liquid.
接着,作为发酵工序,将酵母接种至发酵原料液而进行发酵。发酵所使用的酵母并无特别限定,通常可从酒类的制造所使用的酵母中适当选择而使用。可为上面发酵酵母,也可为下面发酵酵母,就容易应用在大型酿造设备而言,优选为下面发酵酵母。Next, as a fermentation step, yeast is inoculated into the fermentation raw material liquid and fermented. The yeast used for fermentation is not specifically limited, Usually, it can select suitably from the yeast used for manufacture of alcoholic beverages, and can use it. It can be top-fermenting yeast or bottom-fermenting yeast, and it is preferably bottom-fermenting yeast in terms of easy application in large-scale brewing equipment.
进一步地,作为贮酒工序,使所获得的发酵液在贮酒槽中熟成,在0℃左右的低温条件下进行贮藏,使其稳定化之后,作为过滤工序,对熟成后的发酵液进行过滤,由此,将酵母及在该温度区域中不溶的蛋白质等去除,从而能够获得目标发酵啤酒样发泡性饮料。该过滤处理只要为能够将酵母过滤去除的方法即可,例如可列举硅藻土过滤、利用平均孔径为0.4~0.6μm左右的过滤器进行的过滤器过滤等。另外,为了制成所期望的酒精浓度,也可在过滤前或过滤后添加适量的水进行稀释。所获得的发酵啤酒样发泡性饮料通常通过填充工序进行装瓶,作为产品出厂。Furthermore, as a wine storage process, the obtained fermented liquid is matured in a wine storage tank, stored at a low temperature of about 0°C, and stabilized, and then filtered as a filtration process to filter the aged fermented liquid , thereby removing yeast and proteins insoluble in the temperature range, and obtaining the target fermented beer-like sparkling beverage. The filtration treatment may be any method as long as the yeast can be removed by filtration, and examples thereof include diatomaceous earth filtration, filter filtration with a filter having an average pore diameter of about 0.4 to 0.6 μm, and the like. In addition, in order to obtain a desired alcohol concentration, it is also possible to add an appropriate amount of water to dilute before or after filtration. The obtained fermented beer-like sparkling beverage is usually bottled through a filling process and shipped as a product.
此外,在利用酵母的发酵工序以后的工序中,例如通过与含酒精的蒸馏液进行混合,而能够制造相当于酒税法中的甜露酒类的发酵啤酒样发泡性饮料。含酒精的蒸馏液的添加可在用以调整酒精浓度的加水前进行,也可在加水后进行。所添加的含酒精的蒸馏液就可制造具有更好的麦感的发酵啤酒样发泡性饮料而言,优选为麦烈性酒。In addition, in the steps after the fermentation step using yeast, for example, by mixing with alcohol-containing distillate, fermented beer-like sparkling beverages corresponding to liqueurs in the Liquor Tax Law can be produced. The alcoholic distillate can be added before or after adding water to adjust the alcohol concentration. The alcohol-containing distillate to be added is preferably barley spirits because a fermented beer-like sparkling drink having a better barley feel can be produced.
实施例Example
接着,示出实施例及参考例,更详细地说明本发明,但本发明并不限定于以下的实施例等。Next, examples and reference examples are shown, and the present invention will be described in more detail, but the present invention is not limited to the following examples and the like.
[参考例1][Reference example 1]
在非发酵啤酒样发泡性饮料中配合甜菊醇糖苷,调查对呈味的影响。作为甜菊醇糖苷,使用酵素处理甜菊。The effect of adding steviol glycosides to non-fermented beer-like sparkling beverages was investigated. As steviol glycosides, stevia is treated with enzymes.
<非发酵啤酒样发泡性饮料的制造><Manufacture of non-fermented beer-like sparkling beverages>
首先,准备对海库勒斯(Herkules)种啤酒花的啤酒花提取物(产品名:“CO2HopExtract”,Barth-Haas Group公司制造)进行煮沸处理所得物作为原料。First, a hop extract (product name: "CO2HopExtract", manufactured by Barth-Haas Group) of Herkules hops was prepared as a raw material.
在原料水中,分别以饮料的异α酸含量成为0.02g/L的方式配合该煮沸处理所得的啤酒花提取物,以饮料的终浓度成为0.3g/L的方式配合焦糖色素,以饮料的终浓度成为0.50g/L的方式配合磷酸,以饮料的终浓度成为0.35g/L的方式配合柠檬酸钠,且以饮料的终浓度成为0.8g/L的方式配合啤酒香料,将分别配合后的饮料作为基底饮料。In the raw water, the hop extract obtained by the boiling treatment was blended so that the iso-alpha acid content of the beverage became 0.02 g/L, and the caramel color was blended so that the final concentration of the beverage became 0.3 g/L. Phosphoric acid was blended so that the concentration became 0.50g/L, sodium citrate was blended so that the final concentration of the beverage became 0.35g/L, and beer flavor was blended so that the final concentration of the beverage became 0.8g/L. Beverage as a base drink.
对该基底饮料,以饮料中的最终浓度成为表1中记载的量的方式配合酵素处理甜菊(商品名:“SK SWEET FZ”,主成分:甜菊苷及其酵素处理物(85%以上)),而制造非发酵啤酒样发泡性饮料。Enzyme-treated stevia (trade name: "SK SWEET FZ", main component: stevioside and its enzyme-treated product (85% or more)) was blended with the base drink so that the final concentration in the drink became the amount described in Table 1 , while making non-fermented beer-like sparkling beverages.
具体而言,将全部原料配合至原料水中调制被调整为1L的调合液(一次原料液)。接着,向澄清化的一次原料液的液体中吹入二氧化碳,以二氧化碳成为2.9气体容积的方式使其溶解,由此获得非发酵啤酒样发泡性饮料。Specifically, all the raw materials were mixed with raw material water to prepare a prepared liquid (primary raw material liquid) adjusted to 1 L. Next, carbon dioxide was blown into the liquid of the clarified primary raw material liquid, and it was dissolved so that the carbon dioxide became 2.9 gas volumes, thereby obtaining a non-fermented beer-like sparkling drink.
<感官评价><Sensory Evaluation>
所制造的非发酵啤酒样发泡性饮料的感官评价是由啤酒类专业小组成员5名,对饮料的甜味、涩味及类啤酒性,将对照样品(基底饮料)设为3分,将感受强于对照样品者设为5分,将感受弱于对照样品者设为1分,以五个等级评价进行感官评价。所谓类啤酒性是综合地判断是否接近啤酒。在与对照样品相比存在0.5分以上的差的情况下,该饮料被评价为较对照更良好(有改善效果),在存在1分以上的差的情况下,该饮料被评价为较对照显著地良好(有显著的效果)。The sensory evaluation of the produced non-fermented beer-like sparkling beverage was conducted by 5 members of the beer professional panel. For the sweetness, astringency and beer-like properties of the beverage, the control sample (base beverage) was set as 3 points, and the Those who feel stronger than the control sample are set as 5 points, those who feel weaker than the control sample are set as 1 point, and the sensory evaluation is performed with five grades. The so-called beer-like property is to comprehensively judge whether it is close to beer. When there is a difference of 0.5 points or more compared with the control sample, the beverage is evaluated as being better than the control (has an improvement effect), and when there is a difference of 1 point or more, the beverage is evaluated as being significantly better than the control well (significant effect).
将各非发酵啤酒样发泡性饮料的感官评价的结果与酵素处理甜菊的配合量(mg/L)一并示在表1。评价栏中,括号内的数值表示有多名进行该评价的小组成员的情况下的人数。结果,在酵素处理甜菊的配合量为15mg/L(甜菊苷及其酵素处理物的含量为12.75~15mg/L的范围内)的样品1-2、酵素处理甜菊的配合量为30mg/L(甜菊苷及其酵素处理物的含量为25.5~30mg/L的范围内)的样品1-3、酵素处理甜菊的配合量为50mg/L(甜菊苷及其酵素处理物的含量为42.5~50mg/L的范围内)的样品1-4、酵素处理甜菊的配合量为80mg/L(甜菊苷及其酵素处理物的含量为68~80mg/L的范围内)的样品1-5中,与基底饮料(对照样品)相比涩味较强,类啤酒性得到改善。另一方面,在酵素处理甜菊的配合量为120mg/L的样品1-6的非发酵啤酒样发泡性饮料中,虽涩味充分,但强烈感受到作为异味的甜味,类啤酒性较对照样品下降。Table 1 shows the sensory evaluation results of each non-fermented beer-like sparkling beverage together with the compounding amount (mg/L) of the enzyme-treated stevia. In the evaluation column, the numerical values in parentheses indicate the number of people when there are a plurality of team members who performed the evaluation. As a result, in sample 1-2, the compounding amount of enzyme-treated stevia was 15 mg/L (the content of stevioside and its enzyme-treated product was in the range of 12.75 to 15 mg/L), and the compounding amount of enzyme-treated stevia was 30 mg/L ( The content of stevioside and its enzyme-treated product is in the range of 25.5-30 mg/L), the compounding amount of the enzyme-treated stevia is 50 mg/L (the content of stevioside and its enzyme-treated product is 42.5-50 mg/L) In samples 1-4 in the range of L), and in samples 1-5 in which the compounding amount of enzyme-treated stevia was 80 mg/L (the content of stevioside and its enzyme-treated product was in the range of 68-80 mg/L), with the substrate The beverage (control sample) had a stronger astringent taste and improved beer-like properties. On the other hand, in the non-fermented beer-like sparkling beverages of samples 1-6 in which the amount of enzyme-treated stevia was incorporated to be 120 mg/L, although the astringent taste was sufficient, sweetness as an off-flavor was strongly felt, and the beer-like property was relatively low. The control sample declined.
[表1][Table 1]
[参考例2][Reference example 2]
在非发酵啤酒样发泡性饮料中配合甜菊醇糖苷、乙酰磺胺酸钾或蔗糖素,调查对呈味的影响。作为甜菊醇糖苷,使用与参考例1相同的酵素处理甜菊。配合的高甜度甜味料的浓度是以甜度成为相同程度的方式进行调整。The effects on taste of non-fermented beer-like sparkling beverages were investigated by blending steviol glycosides, acesulfame potassium, or sucralose. As steviol glycoside, stevia was treated with the same enzyme as in Reference Example 1. The density|concentration of the high-intensity sweetener to mix|blend is adjusted so that sweetness may become the same level.
具体而言,在参考例1中使用的基底饮料中,以饮料中的最终浓度成为表2中记载的量的方式配合酵素处理甜菊(甜度180)、乙酰磺胺酸钾(甜度200,三荣源公司制造)、及蔗糖素(甜度600,三荣源公司制造),除此以外,以与参考例1相同的方式制造非发酵啤酒样发泡性饮料。Specifically, in the base beverage used in Reference Example 1, enzyme-treated stevia (sweetness 180), acesulfame potassium (sweetness 200, three Rongyuan company), and sucralose (sweetness 600, manufactured by Sanrongyuan company), and non-fermented beer-like sparkling beverages were produced in the same manner as Reference Example 1.
将配合有酵素处理甜菊的样品2-1作为对照样品,除此以外,以与参考例1相同的方式,进行所制造的各非发酵啤酒样发泡性饮料的感官评价,将所得的结果与配合至各饮料中的高甜度甜味料的量(mg/L)一并示在表2。结果,在配合有乙酰磺胺酸钾的样品2-2及配合有蔗糖素的样品2-3的非发酵啤酒样发泡性饮料中,尽管甜度与配合有酵素处理甜菊的样品2-1相同,却感受到异质的甜度,导致类啤酒性下降,另外,涩味也下降。推测其原因在于:甜菊醇糖苷的甜味的表现迟于乙酰磺胺酸钾或蔗糖素,因此,与来自异构化啤酒花提取物的啤酒的独特的苦味相竞争,由此不会感受到成为异味的甜味,而有助于饮用口感及类啤酒性的提高。In addition to using the sample 2-1 containing enzyme-treated stevia as a control sample, sensory evaluation of each non-fermented beer-like sparkling beverage produced was performed in the same manner as in Reference Example 1, and the obtained results were compared with Table 2 shows the amount (mg/L) of the high-intensity sweetener compounded in each drink. As a result, in the non-fermented beer-like sparkling beverages of sample 2-2 containing acesulfame potassium and sample 2-3 containing sucralose, although the sweetness was the same as that of sample 2-1 containing enzyme-treated stevia , but feel the heterogeneous sweetness, resulting in a decrease in beer-like properties, and in addition, the astringency also decreases. The reason for this is presumed to be that the sweetness of steviol glycosides is expressed later than that of acesulfame potassium or sucralose, and therefore, it competes with the unique bitterness of beer derived from isomerized hop extracts, so that it will not be perceived as an off-flavor sweetness, which contributes to the improvement of drinking taste and beer-like property.
[表2][Table 2]
[实施例1][Example 1]
在配合有甜菊醇糖苷的啤酒样发泡性饮料中,进一步配合聚葡萄糖,调查对呈味的影响。作为甜菊醇糖苷,配合与参考例1相同的酵素处理甜菊,其量与参考例1中类啤酒性的评价最高的样品1-3相同(30mg/L)。In beer-like sparkling beverages containing steviol glycosides, polydextrose was further compounded, and the influence on taste was investigated. As steviol glycoside, the same enzyme-treated stevia as in Reference Example 1 was blended in the same amount (30 mg/L) as Sample 1-3 with the highest evaluation of beer-like properties in Reference Example 1.
具体而言,在参考例1中使用的基底饮料中,以饮料中的最终浓度成为表3中记载的量的方式分别配合酵素处理甜菊、及聚葡萄糖剂(产品名:“Litesse HF”,食物纤维纯度:95%,Danisco公司制造),除此以外,以与参考例1相同的方式制造非发酵啤酒样发泡性饮料。表3的“聚葡萄糖浓度[g/L]”中,上段为配合至饮料中的聚葡萄糖剂的浓度,下段为实际上配合至饮料中的聚葡萄糖的浓度。Specifically, in the base beverage used in Reference Example 1, enzyme-treated stevia and polydextrose agent (product name: "Litesse HF", food Fiber purity: 95%, manufactured by Danisco Corporation), a non-fermented beer-like sparkling beverage was produced in the same manner as in Reference Example 1 except that. In "Polydextrose Concentration [g/L]" in Table 3, the upper row is the concentration of the polydextrose agent blended into the drink, and the lower row is the concentration of the polydextrose actually blended into the drink.
将仅配合有酵素处理甜菊的样品3-1作为对照样品,除此以外,以与参考例1相同的方式,进行所制造的各非发酵啤酒样发泡性饮料的感官评价,将所得的结果示在表3。如表3所示,在聚葡萄糖的配合量为4.75g/L以上的样品3-3~样品3-6的非发酵啤酒样发泡性饮料中,苦味或甜味虽与样品3-1的非发酵啤酒样发泡性饮料并无多大差异,但类啤酒性得到改善,进一步,根据小组成员的评价可知,啤酒味道温和。尤其是,在聚葡萄糖的配合量为4.75~19g/L样品3-3~样品3-5中,类啤酒性得到显著改善。根据这些结果确认:通过并用甜菊醇糖苷与聚葡萄糖,能够改善类啤酒性。Except for the sample 3-1 containing only the enzyme-treated stevia as a control sample, sensory evaluation of each non-fermented beer-like sparkling beverage produced was carried out in the same manner as in Reference Example 1, and the obtained results were compared. shown in Table 3. As shown in Table 3, in the non-fermented beer-like sparkling beverages of samples 3-3 to 3-6 in which the amount of polydextrose blended was 4.75 g/L or more, the bitterness or sweetness was comparable to that of sample 3-1. There was not much difference in non-fermented beer-like sparkling beverages, but beer-like properties were improved, and further, the beer tasted mild according to the panelists' evaluations. In particular, in samples 3-3 to 3-5 where the amount of polydextrose blended was 4.75 to 19 g/L, the beer-like properties were remarkably improved. From these results, it was confirmed that beer-like properties can be improved by using steviol glycoside and polydextrose in combination.
[表3][table 3]
在此,针对各样品,进一步对温和度进行感官评价。此处,“温和”是刺激的反义词,是指顺滑且一致的味感。具体而言,由相同的啤酒类专业小组成员5名,与对照样品(样品3-1)进行比较,将较对照样品温和度提升者评价为“○”,将与对照样品为相同程度者评价为“△”,将较对照样品下降(=有刺激感)者评价为“×”。将对各样品进行各评价的小组成员的人数示在表4。Here, about each sample, the mildness was further sensory-evaluated. Here, "mild" is the opposite of sharp, referring to a smooth and consistent taste. Specifically, 5 members of the same professional beer group were compared with the control sample (sample 3-1), and those who were milder than the control sample were evaluated as "○", and those who were the same as the control sample were evaluated. It was "Δ", and those that were lower (=stimulating) than the control sample were evaluated as "x". Table 4 shows the number of panelists who evaluated each sample.
[表4][Table 4]
如表4所示,在聚葡萄糖的配合量为4.75g/L以上的样品3-3~样品3-6的非发酵啤酒样发泡性饮料中,过半数的小组成员判定为温和。根据该结果,推测:在样品3-3~样品3-6的非发酵啤酒样发泡性饮料中,相较在仅配合有酵素处理甜菊的样品3-1,类啤酒性得到改善,其原因在于:通过聚葡萄糖,刺激减少,香味实现一致性,由此实现类似啤酒味道的一致性。推测:尤其是通过聚葡萄糖,使来自甜菊醇糖苷的涩味质量得到改善从而赋予来自涩味的适度的饮用口感的情况也有助于类啤酒性的改善。另一方面,在聚葡萄糖的配合量为38g/L样品3-6中,温和度虽有所改善,但类啤酒性下降,因此,若变得过于温和,则味道变平淡而出现不期望的倾向。As shown in Table 4, in the non-fermented beer-like sparkling beverages of samples 3-3 to 3-6 in which the amount of polydextrose blended was 4.75 g/L or more, more than half of the panelists judged it as mild. Based on the results, it is speculated that the beer-like properties of the non-fermented beer-like sparkling beverages of samples 3-3 to 3-6 were improved compared to sample 3-1 containing only the enzyme-treated stevia. In that: by polydextrose, the irritation is reduced, and the aroma is achieved in consistency, thereby achieving a beer-like consistency in taste. It is speculated that especially polydextrose improves the quality of astringent taste derived from steviol glycosides and imparts a moderate drinking texture derived from astringent taste, which also contributes to the improvement of beer-like properties. On the other hand, in the sample 3-6 with the blending amount of polydextrose being 38 g/L, the mildness was improved, but the beer-like property was decreased. Therefore, if it became too mild, the taste would become flat and undesired. tendency.
[实施例2][Example 2]
对通过并用配合甜菊醇糖苷与聚葡萄糖而产生的类啤酒性的改善效果是否也可通过甜菊醇糖苷与其它水溶性食物纤维的并用配合而获得进行调查。作为甜菊醇糖苷,配合与参考例1相同的酵素处理甜菊,其量与参考例1中类啤酒性的评价最高的样品1-3相同(30mg/L)。It was investigated whether the improvement effect of the beer-like property by combining steviol glycoside and polydextrose can also be obtained by combining steviol glycoside and other water-soluble dietary fiber. As steviol glycoside, the same enzyme-treated stevia as in Reference Example 1 was blended in the same amount (30 mg/L) as Sample 1-3 with the highest evaluation of beer-like properties in Reference Example 1.
具体而言,在参考例1中使用的基底饮料中,以饮料中的水溶性食物纤维的最终浓度成为9.5g/L的方式分别配合酵素处理甜菊、及聚葡萄糖剂(产品名:“Litesse HF”,食物纤维纯度:95%,Danisco公司制造)或难消化性糊精剂(产品名:“E纤维”,食物纤维纯度:90%,松谷化学公司制造),除此以外,以与参考例1相同的方式制造非发酵啤酒样发泡性饮料。Specifically, in the base beverage used in Reference Example 1, enzyme-treated stevia and polydextrose agent (product name: "Litesse HF ", dietary fiber purity: 95%, manufactured by Danisco Corporation) or indigestible dextrin (product name: "E fiber", dietary fiber purity: 90%, manufactured by Matsutani Chemical Co., Ltd.) 1 The non-fermented beer-like sparkling beverage is produced in the same manner.
将配合有酵素处理甜菊的样品4-1作为对照样品,除此以外,以与参考例1相同的方式,进行所制造的各非发酵啤酒样发泡性饮料的类啤酒性的感官评价。另外,将配合有酵素处理甜菊的样品4-1作为对照样品,除此以外,以与参考例1相同的方式,进行所制造的各非发酵啤酒样发泡性饮料的温和度的感官评价。将结果示在表5。如表5所示,在配合有难消化性糊精的样品4-2的非发酵啤酒样发泡性饮料中,与配合有聚葡萄糖的样品4-3的非发酵啤酒样发泡性饮料不同,未观察到温和度或类啤酒性的改善。根据该结果确认:通过与甜菊醇糖苷并用配合而产生的类啤酒性改善效果是配合有水溶性食物纤维中的尤其是聚葡萄糖的情况下所特有的效果。In the same manner as in Reference Example 1, sensory evaluation of the beer-like properties of each of the produced non-fermented beer-like sparkling beverages was performed except that the sample 4-1 containing enzyme-treated stevia was used as a control sample. In addition, the sensory evaluation of the mildness of each non-fermented beer-like sparkling beverage produced was performed in the same manner as in Reference Example 1 except that the sample 4-1 containing enzyme-treated stevia was used as a control sample. The results are shown in Table 5. As shown in Table 5, the non-fermented beer-like sparkling beverage of sample 4-2 containing indigestible dextrin was different from the non-fermented beer-like sparkling beverage of sample 4-3 containing polydextrose. , no improvement in mildness or beer-likeness was observed. From these results, it was confirmed that the effect of improving beer-like properties by combining steviol glycosides with steviol glycosides is an effect unique to the case where polydextrose, in particular, is added among water-soluble dietary fibers.
[表5][table 5]
Claims (5)
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