CN110157598B - Automated loading device for microplates - Google Patents
Automated loading device for microplates Download PDFInfo
- Publication number
- CN110157598B CN110157598B CN201910505094.0A CN201910505094A CN110157598B CN 110157598 B CN110157598 B CN 110157598B CN 201910505094 A CN201910505094 A CN 201910505094A CN 110157598 B CN110157598 B CN 110157598B
- Authority
- CN
- China
- Prior art keywords
- tip head
- microplate
- tip
- head
- material storage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000011521 glass Substances 0.000 claims abstract description 23
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 14
- 230000001681 protective effect Effects 0.000 claims abstract description 11
- 239000002699 waste material Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 34
- 239000000463 material Substances 0.000 claims description 26
- 238000002347 injection Methods 0.000 claims description 10
- 239000007924 injection Substances 0.000 claims description 10
- 238000007906 compression Methods 0.000 claims description 9
- 230000006835 compression Effects 0.000 claims description 8
- 239000011148 porous material Substances 0.000 description 19
- 239000000758 substrate Substances 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B25—HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
- B25J—MANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
- B25J15/00—Gripping heads and other end effectors
Landscapes
- Engineering & Computer Science (AREA)
- Robotics (AREA)
- Mechanical Engineering (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
微孔板的自动化加样装置，包括百级层流、玻璃防护罩、台板、加样头、机械手，百级层流，所述玻璃防护罩和台板构成工作区域，所述台板上设有tip头存料盘、待加样载台、微孔板载台、tip头废料框、机械手，所述加样头安装于机器手末端，tip头存料盘上设有tip头，微孔板载台上设有微孔板。本发明能实现自动化加样，降低实验操作者的劳动程度，加样速度、加样准确度较现有技术均得到极大层度的提高；对目标微孔加注特定的试剂，不易混淆；加样的环境可控性高。
The automatic loading device for microplate includes a hundred-level laminar flow, a glass protective cover, a platform, a loading head, and a manipulator. The hundred-level laminar flow, the glass protective cover and the platform constitute a working area, the platform is provided with a tip head storage tray, a sample loading platform, a microplate loading platform, a tip head waste frame, and a manipulator, the loading head is installed at the end of the manipulator, the tip head storage tray is provided with a tip head, and the microplate loading platform is provided with a microplate. The present invention can realize automatic loading, reduce the labor of the experimental operator, and greatly improve the loading speed and loading accuracy compared with the prior art; add specific reagents to the target micropores, which is not easy to be confused; and the loading environment is highly controllable.
Description
Technical Field
The invention relates to the field of experimental instruments, in particular to an automatic sample adding device of a micro-pore plate.
Background
PCR sample addition is generally performed by using 96-well or 384-well microplates, and during operation, since each microplates have many holes, the hole-to-hole spacing is very small, and the hole in the middle of the well plate is difficult to distinguish, sample addition confusion or omission easily occurs during sample addition by using a pipette, and the experimental result is abnormal. Meanwhile, in the process of using the pipettor, the measurement adjusting part may be touched by manual operation, so that the sample adding amounts of different holes are inconsistent, and experimental errors are increased.
In addition, for the sample application of special samples, such as RNA/DNA, etc., the degradation of the sample needs to be reduced in a sterile and enzyme-free low-temperature environment, while the general sample application environment is open, and the pipetting device and the surrounding environment cannot ensure the low-temperature sterile and enzyme-free environment. In the experimental process, a plurality of micro-pore plates to be sampled are frequently encountered, the condition of a plurality of indexes of each micro-pore plate is adopted, the working intensity of experimental staff can be greatly increased by manual sampling, mental and physical fatigue is caused, and sampling errors are increased. Meanwhile, subjective factors are not eliminated by manual sample addition, and the authenticity of the result is reduced.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects of the prior art and providing the device which can improve the operation experience of experiment operators, improve the experiment efficiency, improve the reliability of the experiment result and realize the automatic sample adding of the micro-pore plate.
The technical scheme includes that the automatic sample adding device for the micro-pore plate comprises hundred-level laminar flow, a glass protection cover, a platen, a sample adding head and a manipulator, wherein the glass protection cover and the platen form a working area, a tip head storage tray, a sample loading platform to be added, a micro-pore plate carrying platform, a tip head waste frame and the manipulator are arranged on the platen, the sample adding head is arranged at the tail end of the manipulator, the tip head is arranged on the tip head storage tray, and the micro-pore plate is arranged on the micro-pore plate carrying platform.
Further, the glass protective cover comprises a movable glass door in three directions and a fixed glass door in one direction, and hundred-level laminar flow generates vertically downward laminar flow wind.
Further, the tip head stock disc comprises a tip head stock plate and a stock turntable, the tip head is mounted on the tip head stock plate, the stock turntable is mounted on the platen and driven by a servo motor, a groove for mounting the tip head stock plate is formed in the stock turntable, the tip head stock plate is fixed in the groove, the tip head stock plate 31 is mounted in a pressed-in mode, the tip head stock plate and the micro-pore plate have the same hole number and hole distance, and the tip head is inserted into the stock holes of the tip head stock plate in advance by manpower and then is placed in the groove of the turntable.
Further, 4 material storage plates distributed in four quadrants are arranged on the material storage turntable.
Further, the loading platform to be loaded comprises a plurality of reagent tube jacks, a cold water injection port I and a cold water discharge port I, and the reagent tubes to be loaded are inserted into the reagent tube jacks.
Furthermore, a water storage structure is designed in the sample loading platform to be loaded.
Further, the micro-pore plate carrier comprises a micro-pore plate positioning sink, a cold water injection port II and a cold water discharge port II.
Further, the micropore plate and the micropore plate positioning sink skip through fit, the interior of the micropore plate carrier is hollow, and the number of the micropore plate and the micropore plate positioning sink is the same.
Further, the sample adding head comprises a mounting substrate, a micro gear pump, an electromagnet, a tip head material returning rod, a tip head needle mouth seat, a compression spring, a needle mouth seat mounting block, a tip head needle mouth and a tip head.
Further, the micro gear pump is arranged on one side of the mounting substrate and is connected with the needle nozzle seat mounting block through a hose or a hard tube, the electromagnet is arranged on the other side of the mounting substrate and is used for providing power for eliminating used tip heads, the needle nozzle seat mounting block is embedded in the mounting substrate, the tip head needle nozzle seat is arranged on the needle nozzle seat mounting block, the tip head needle nozzle is arranged in the tip head needle nozzle seat, the tip head needle nozzle can slide up and down in the tip head needle nozzle seat, the tip head needle nozzle is propped at the lowest position by a compression spring, one end of a tip head material returning rod is fixed at the tail end of an electromagnet extending shaft, and the other end of the tip head material returning rod is sleeved on the tip head needle nozzle.
The invention has the advantages that the automatic sample adding can be realized, the labor degree of experiment operators is reduced, the sample adding speed and the sample adding accuracy are improved greatly compared with the prior art, the specific reagent is added to the target micropore, the confusion is not easy, and the sample adding environment controllability is high.
Drawings
FIG. 1 is a schematic three-dimensional structure of an embodiment of the present invention;
FIG. 2 is a schematic diagram of a two-dimensional structure according to an embodiment of the present invention;
FIG. 3 is a side view of the embodiment of FIG. 2;
FIG. 4 is a schematic three-dimensional structure of the sample application head of FIG. 1;
FIG. 5 is a two-dimensional cross-sectional view of the sample addition head of FIG. 2.
In the figure, 1-hundred-level laminar flow, a 2-glass protective cover, a 21-movable glass door, a 22-fixed glass door, a 3-tip head stock tray, a 30-tip head, a 31-tip head stock plate, a 32-stock turntable, a 4-platen, a 5-sample loading platform, a 51-reagent tube inserting hole, a 52-cold water injection opening I, a 53-cold water discharge opening I, a 6-micropore plate loading platform, a 61-micropore plate, a 62-cold water injection opening II, a 63-cold water discharge opening II, a 64-micropore plate positioning sink, a 7-tip head waste frame, an 8-sample loading head, a 81-mounting substrate, 82-trace amount of electromagnets, 84-tip head material returning rods, 85-tip head needle mouth seats, 86-compression springs, 87-needle mouth seat mounting blocks, 88-tip head needle mouths and 9-manipulators are shown.
Detailed Description
The invention is described in further detail below with reference to the drawings and examples.
The embodiment comprises a hundred-level laminar flow 1, a glass protection cover 2, a platen 4, a sample adding head 8 and a manipulator 9, wherein the hundred-level laminar flow 1, the glass protection cover 2 and the platen 4 form a clean experiment area without bacteria and enzymes, the platen 4 is provided with a tip head storage tray 3, a sample adding carrying platform 5, a micro-pore plate carrying platform 6 and a tip head waste material frame 7, the manipulator 9, the sample adding head 8 is arranged at the tail end of the manipulator 9, the tip head storage tray 3 is provided with a tip head 30, the micro-pore plate carrying platform 6 is provided with a micro-pore plate 61, when sample adding is executed, the manipulator 9 firstly automatically installs a new tip head at the tip head storage tray 3, then moves to the position of the sample adding carrying platform 5 and samples, samples are taken out, then moves to the position of the micro-pore plate carrying platform 6, the samples are injected into micro-pores of the specified micro-pore plate 61, finally the sample adding head 8 is transferred to the position above the tip head waste material recycling frame 7, the tip head 30 used by the tip head 30 of the sample adding head 8 is removed automatically, and the tip head 30 falls into the tip head waste material frame 7 is completed, and the sample adding cycle is completed.
The glass protective cover 2 comprises a movable glass door 21 in three directions and a fixed glass door 22 in one direction, the hundred-level laminar flow 1 generates vertically downward laminar flow wind, and meanwhile, clean wind flows out of a gap with a certain height reserved between the glass protective cover 2 and the bedplate 4, so that the air pressure in the glass protective cover 2 is higher than the external air pressure, and the inside of the glass protective cover 2 is under the protection of positive pressure and clean wind.
The tip head stock disc 3 comprises a tip head stock plate 31 and a stock turntable 32, and the tip head 30 is arranged on the tip head stock plate 31. The stock turntable 31 is mounted above the platen 4 and is driven by a servo motor. The material storage turntable 32 is provided with 4 grooves for installing the tip head material storage plates 31, the tip head material storage plates 31 are fixed in the grooves, and the tip head material storage plates 31 are installed in a pressed-in mode, so that the disassembly is convenient. the tip head stock plate 31 and the micro-hole plate 61 have the same hole number and hole pitch, and the tip head 30 is manually inserted into the stock hole of the tip head stock plate 3 in advance and then put into the turntable groove. 4 stock plates can be pre-stored on the stock turntable 32, the manipulator 9 takes the tip head 30 in a fixed area every time, in fig. 2, the first quadrant of the stock turntable 32 (i.e. the position where the tip head stock plate is located is indicated by the serial number 31), when the tip head 30 in the tip head stock plate 31 is completely used up, the stock turntable 32 rotates 90 degrees clockwise or anticlockwise, so that a full tip head stock plate 31 moves to the area, and the empty tip head stock plate 31 moves out of the area, and according to the cycle, the 4 tip head stock plates 31 can be completely used up and then are stopped for feeding, and the empty tip head stock plate transferred to the second quadrant or the third quadrant can be replaced by full material, so that the stock turntable 32 is always in a material state, thereby avoiding unnecessary midway stopping.
The loading platform 5 to be loaded comprises a plurality of reagent tube jacks 51, a cold water injection port I52 and a cold water discharge port I53, and reagent tubes to be loaded are inserted into the reagent tube jacks 51. The water storage structure is designed in the sample loading platform 5, when the reagent needs to be kept at a low temperature environment of about 4 ℃, a cold water circulation mode can be started, a cold water machine (not shown in the figure) is arranged below the platen 4, cold water is injected through the cold water injection port I52, the cold water is discharged through the cold water discharge port I53, and the cold water is slowly circulated, so that the reagent pipe is in a cold water bath constant temperature environment.
The microplate carrier 6 comprises a microplate positioning sink 64, a cold water injection port II62 and a cold water discharge port II63. The microplate 61 is pressed into the microplate positioning sink 64 in an interference fit manner, the microplate carrier 6 is hollow, when the microplate 61 sample needs to be maintained in a low-temperature environment of about 4 ℃, cold water can be injected through the cold water injection port II62 and discharged through the cold water discharge port II63, and the microplate is slowly circulated, so that the bottom of the microplate 61 is immersed in the cold water and is in a cold water bath state, and the cold water is also supplied by a cold water machine (not shown in the figure). Two locating sink grooves 64 of the micro-pore plate are provided.
The sample loading head 8 includes a mounting board 81, a micro gear pump 82, an electromagnet 83, a tip head withdrawal lever 84, a tip head tip seat 85, a compression spring 86, a tip seat mounting block 87, a tip head tip 88, and a tip head 30.
The micro gear pump 82 is mounted on one side of the mounting board 81, and is connected to the nozzle mount block 87 via a hose or a hard tube.
The electromagnet 83 is mounted on the other side of the mounting substrate 81, the needle holder mounting 87 is embedded in the mounting substrate 81, the tip needle holder 85 is mounted on the needle holder mounting 87, the tip needle holder 88 is mounted in the tip needle holder 85, the tip needle holder 88 can slide up and down in the tip needle holder 85, the compression spring 86 is mounted in a gap between the tip needle holder 88 and the tip needle holder 85, the tip needle holder 88 is propped against the lowest position by the compression spring 86, the tip needle holder 88 is connected with the tip head 30 through an interference surface, the contact surface is a section of mutually matched conical surface with a guide angle, one end of the tip material returning rod 84 is fixed at the end of the extending shaft of the electromagnet 83, and the other end is sleeved on the tip needle holder 88.
During the working process, when the tip 30 is installed, the manipulator 9 drives the tip needle and mouth 88 to align with one tip 30 in the tip stock plate 31, and then moves downwards to enable the tip needle and mouth 88 to be inserted into the tip 30, the contact surface of the tip needle and mouth 88 and the tip 30 is a section of mutually matched conical surface with a guide angle, and when the tip needle and mouth 88 is inserted into the tip 30 at a certain speed, the tip needle and mouth 88 is tightly attached together due to expansion force generated by wedging action, so that the tip 30 is installed. In this process, the tip needle tip 88 is moved upward by a certain distance under the action of the compression spring 86 to cushion the impact, avoiding rigid contact between moving parts, and protecting the robot arm. When the tip head 30 is required to be removed after sample addition, the electromagnet 83 is electrified, and the extension shaft of the electromagnet 83 extends out, so that the ring of one end of the tip head material returning rod 84 sleeved on the tip head needle mouth 88 is driven to move downwards, and the tip head 30 is pushed out of the tip head needle mouth 88.
Various modifications and variations of the embodiments of the present invention may be made by those skilled in the art, and, if such modifications and variations are within the scope of the claims and their equivalents, they are also within the scope of the patent of the present invention.
What is not described in detail in the specification is prior art known to those skilled in the art.
Claims (6)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910505094.0A CN110157598B (en) | 2019-06-12 | 2019-06-12 | Automated loading device for microplates |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910505094.0A CN110157598B (en) | 2019-06-12 | 2019-06-12 | Automated loading device for microplates |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110157598A CN110157598A (en) | 2019-08-23 |
| CN110157598B true CN110157598B (en) | 2025-01-28 |
Family
ID=67628673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910505094.0A Active CN110157598B (en) | 2019-06-12 | 2019-06-12 | Automated loading device for microplates |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110157598B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110215941A (en) * | 2019-07-05 | 2019-09-10 | 中南大学湘雅医院 | Microwell plate is loaded indicating unit and microwell plate is loaded auxiliary device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1090280A (en) * | 1996-09-18 | 1998-04-10 | Olympus Optical Co Ltd | Liquid dispenser and distributing device |
| KR20110081718A (en) * | 2010-01-08 | 2011-07-14 | (주)바이오니아 | Biological material automatic purification device |
| CN204855542U (en) * | 2015-06-30 | 2015-12-09 | 中国科学院苏州生物医学工程技术研究所 | Blood group is application of sample device for appearance |
| CN108490205A (en) * | 2018-04-10 | 2018-09-04 | 深圳市博辰智控有限公司 | A kind of microwell plate sample adding system and method |
| CN210560409U (en) * | 2019-06-12 | 2020-05-19 | 中南大学湘雅医院 | An automatic sample loading device for microplates |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691498B (en) * | 2014-01-06 | 2015-05-27 | 广州市刑事科学技术研究所 | Liquid-moving machine and method of moving liquid by same |
| CN106582907B (en) * | 2015-10-15 | 2021-05-18 | 常州福生生物技术有限公司 | Pipette capable of automatically withdrawing suction head |
| US11167282B2 (en) * | 2017-01-31 | 2021-11-09 | HighRes Biosolutions, Inc. | Auto-pipetting apparatus and method |
| CN106554914A (en) * | 2017-02-09 | 2017-04-05 | 上海亿康医学检验所有限公司 | A kind of automatic nucleic acid extraction system |
-
2019
- 2019-06-12 CN CN201910505094.0A patent/CN110157598B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1090280A (en) * | 1996-09-18 | 1998-04-10 | Olympus Optical Co Ltd | Liquid dispenser and distributing device |
| KR20110081718A (en) * | 2010-01-08 | 2011-07-14 | (주)바이오니아 | Biological material automatic purification device |
| CN204855542U (en) * | 2015-06-30 | 2015-12-09 | 中国科学院苏州生物医学工程技术研究所 | Blood group is application of sample device for appearance |
| CN108490205A (en) * | 2018-04-10 | 2018-09-04 | 深圳市博辰智控有限公司 | A kind of microwell plate sample adding system and method |
| CN210560409U (en) * | 2019-06-12 | 2020-05-19 | 中南大学湘雅医院 | An automatic sample loading device for microplates |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110157598A (en) | 2019-08-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11524287B2 (en) | Automatic pipetting device for transferring samples and/or reagents and method for transferring liquid samples and/or reagents | |
| US6428752B1 (en) | Cleaning deposit devices that form microarrays and the like | |
| US6299840B1 (en) | Automatic testing apparatus | |
| US7541001B2 (en) | Automatic pipetting and analyzing device | |
| US20030108868A1 (en) | Apparatus and method for aligning microarray printing head | |
| EP3597728B1 (en) | Cell transfer device and cell transfer method | |
| JP2006010700A (en) | Apparatus and method for spotting substrate | |
| US12019001B2 (en) | Fluid transport system | |
| CN110270389B (en) | Pipetting machine | |
| US20210071166A1 (en) | Biological material extraction apparatus | |
| CN110157598B (en) | Automated loading device for microplates | |
| CN116298353A (en) | Device for micro-volume liquid pipetting operation | |
| CN210560409U (en) | An automatic sample loading device for microplates | |
| EP2034319A2 (en) | Separation Apparatus | |
| CN104946526A (en) | Nucleic acid analysis device and nucleic acid analysis method | |
| US20110132109A1 (en) | Device and process for the formation of microdepositions | |
| IL152050A (en) | Method and device for producing biopolymer arrays | |
| CN206521467U (en) | The volley of rifle fire for miniature full-automatic chemical luminescence detector | |
| CN113416636A (en) | Biological experiment operation equipment and use method thereof | |
| JP2016015922A (en) | Nucleic acid analyzer | |
| CN210323058U (en) | A sample loading head for microplates | |
| CN210907134U (en) | Micro-fluidic chip's belt cleaning device | |
| CN106908617B (en) | Method for transferring liquid volume in analyzer | |
| EP4516403A1 (en) | A dosing tool for liquid handling | |
| JP4042767B2 (en) | Dispensing device and dispensing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhang Shuiting Inventor after: Qiu Yuanzheng Inventor after: Liu Yong Inventor after: Li Guo Inventor after: Liu Chao Inventor after: Wang Yunyun Inventor after: Lu Shanli Inventor after: Looking forward Inventor after: Gong Jing Inventor before: Qiu Yuanzheng Inventor before: Liu Yong Inventor before: Zhang Shuiting Inventor before: Li Guo Inventor before: Liu Chao Inventor before: Wang Yunyun Inventor before: Lu Shanli Inventor before: Looking forward Inventor before: Gong Jing |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |