CN110146622A - The measuring method and quality assessment method of mangiferin in a kind of common bombax flower - Google Patents
The measuring method and quality assessment method of mangiferin in a kind of common bombax flower Download PDFInfo
- Publication number
- CN110146622A CN110146622A CN201910511187.4A CN201910511187A CN110146622A CN 110146622 A CN110146622 A CN 110146622A CN 201910511187 A CN201910511187 A CN 201910511187A CN 110146622 A CN110146622 A CN 110146622A
- Authority
- CN
- China
- Prior art keywords
- mangiferin
- common bombax
- bombax flower
- solution
- methanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- AEDDIBAIWPIIBD-ZJKJAXBQSA-N mangiferin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=C(OC=2C(=CC(O)=C(O)C=2)C2=O)C2=C1O AEDDIBAIWPIIBD-ZJKJAXBQSA-N 0.000 title claims abstract description 110
- 241000219071 Malvaceae Species 0.000 title claims abstract description 72
- YWQSXCGKJDUYTL-UHFFFAOYSA-N Mangiferin Natural products CC(CCC=C(C)C)C1CC(C)C2C3CCC4C(C)(C)CCCC45CC35CCC12C YWQSXCGKJDUYTL-UHFFFAOYSA-N 0.000 title claims abstract description 55
- 229940043357 mangiferin Drugs 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 37
- 238000001303 quality assessment method Methods 0.000 title claims abstract description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 77
- 239000013558 reference substance Substances 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 6
- 239000000706 filtrate Substances 0.000 claims abstract description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 3
- 239000000945 filler Substances 0.000 claims description 2
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 claims description 2
- 239000000377 silicon dioxide Substances 0.000 claims description 2
- 238000009210 therapy by ultrasound Methods 0.000 claims description 2
- 235000003301 Ceiba pentandra Nutrition 0.000 claims 1
- 244000146553 Ceiba pentandra Species 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 4
- 238000004364 calculation method Methods 0.000 abstract description 2
- 238000003255 drug test Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 34
- 239000000523 sample Substances 0.000 description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 239000000126 substance Substances 0.000 description 8
- 238000011160 research Methods 0.000 description 7
- 229930182470 glycoside Natural products 0.000 description 6
- 238000004128 high performance liquid chromatography Methods 0.000 description 6
- 239000003814 drug Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 150000002338 glycosides Chemical class 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 235000004936 Bromus mango Nutrition 0.000 description 4
- 241001093152 Mangifera Species 0.000 description 4
- 235000014826 Mangifera indica Nutrition 0.000 description 4
- 235000009184 Spondias indica Nutrition 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 230000003110 anti-inflammatory effect Effects 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000000144 pharmacologic effect Effects 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 208000004880 Polyuria Diseases 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 235000006708 antioxidants Nutrition 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000035619 diuresis Effects 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 238000002137 ultrasound extraction Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- YQUVCSBJEUQKSH-UHFFFAOYSA-N 3,4-dihydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 description 2
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 2
- 206010037660 Pyrexia Diseases 0.000 description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- 230000036592 analgesia Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 230000002141 anti-parasite Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 239000003096 antiparasitic agent Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 230000006837 decompression Effects 0.000 description 2
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 208000001848 dysentery Diseases 0.000 description 2
- XHCADAYNFIFUHF-TVKJYDDYSA-N esculin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC(C(=C1)O)=CC2=C1OC(=O)C=C2 XHCADAYNFIFUHF-TVKJYDDYSA-N 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- 208000014617 hemorrhoid Diseases 0.000 description 2
- 230000002218 hypoglycaemic effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
- 210000002429 large intestine Anatomy 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004112 neuroprotection Effects 0.000 description 2
- 230000036407 pain Effects 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000004224 protection Effects 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- -1 scopolactone Chemical compound 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- QAIPRVGONGVQAS-DUXPYHPUSA-N trans-caffeic acid Chemical compound OC(=O)\C=C\C1=CC=C(O)C(O)=C1 QAIPRVGONGVQAS-DUXPYHPUSA-N 0.000 description 2
- 230000010415 tropism Effects 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- ACEAELOMUCBPJP-UHFFFAOYSA-N (E)-3,4,5-trihydroxycinnamic acid Natural products OC(=O)C=CC1=CC(O)=C(O)C(O)=C1 ACEAELOMUCBPJP-UHFFFAOYSA-N 0.000 description 1
- FTVWIRXFELQLPI-ZDUSSCGKSA-N (S)-naringenin Chemical compound C1=CC(O)=CC=C1[C@H]1OC2=CC(O)=CC(O)=C2C(=O)C1 FTVWIRXFELQLPI-ZDUSSCGKSA-N 0.000 description 1
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 1
- MIJYXULNPSFWEK-GTOFXWBISA-N 3beta-hydroxyolean-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C MIJYXULNPSFWEK-GTOFXWBISA-N 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 1
- 239000003390 Chinese drug Substances 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- JKLISIRFYWXLQG-UHFFFAOYSA-N Epioleonolsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4CCC3C21C JKLISIRFYWXLQG-UHFFFAOYSA-N 0.000 description 1
- GMRNMZUSKYJXGJ-UHFFFAOYSA-N Fraxetin Natural products C1=CC(=O)C(=O)C2=C1C=C(OC)C(O)=C2O GMRNMZUSKYJXGJ-UHFFFAOYSA-N 0.000 description 1
- 239000005639 Lauric acid Substances 0.000 description 1
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 1
- YBRJHZPWOMJYKQ-UHFFFAOYSA-N Oleanolic acid Natural products CC1(C)CC2C3=CCC4C5(C)CCC(O)C(C)(C)C5CCC4(C)C3(C)CCC2(C1)C(=O)O YBRJHZPWOMJYKQ-UHFFFAOYSA-N 0.000 description 1
- MIJYXULNPSFWEK-UHFFFAOYSA-N Oleanolinsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C MIJYXULNPSFWEK-UHFFFAOYSA-N 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
- JMFSHKGXVSAJFY-UHFFFAOYSA-N Saponaretin Natural products OCC(O)C1OC(Oc2c(O)cc(O)c3C(=O)C=C(Oc23)c4ccc(O)cc4)C(O)C1O JMFSHKGXVSAJFY-UHFFFAOYSA-N 0.000 description 1
- MEGPURSNXMUDAE-UHFFFAOYSA-N Scopoline Natural products C1C(O2)CC3N(C)C1C2C3O MEGPURSNXMUDAE-UHFFFAOYSA-N 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- MOZJVOCOKZLBQB-UHFFFAOYSA-N Vitexin Natural products OCC1OC(Oc2c(O)c(O)cc3C(=O)C=C(Oc23)c4ccc(O)cc4)C(O)C(O)C1O MOZJVOCOKZLBQB-UHFFFAOYSA-N 0.000 description 1
- ZFLXCZJBYSPSKU-CRHREIPCSA-N [(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] (e,1e)-5-methylsulfinyl-n-sulfooxypent-4-enimidothioate Chemical compound CS(=O)\C=C\CC\C(=N/OS(O)(=O)=O)S[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O ZFLXCZJBYSPSKU-CRHREIPCSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- FGDQGIKMWOAFIK-UHFFFAOYSA-N acetonitrile;phosphoric acid Chemical compound CC#N.OP(O)(O)=O FGDQGIKMWOAFIK-UHFFFAOYSA-N 0.000 description 1
- QNHQEUFMIKRNTB-UHFFFAOYSA-N aesculetin Natural products C1CC(=O)OC2=C1C=C(O)C(O)=C2 QNHQEUFMIKRNTB-UHFFFAOYSA-N 0.000 description 1
- QUQPHWDTPGMPEX-UTWYECKDSA-N aurantiamarin Natural products COc1ccc(cc1O)[C@H]1CC(=O)c2c(O)cc(O[C@@H]3O[C@H](CO[C@@H]4O[C@@H](C)[C@H](O)[C@@H](O)[C@H]4O)[C@@H](O)[C@H](O)[C@H]3O)cc2O1 QUQPHWDTPGMPEX-UTWYECKDSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 235000004883 caffeic acid Nutrition 0.000 description 1
- 229940074360 caffeic acid Drugs 0.000 description 1
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 1
- 229940074393 chlorogenic acid Drugs 0.000 description 1
- 235000001368 chlorogenic acid Nutrition 0.000 description 1
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 1
- QAIPRVGONGVQAS-UHFFFAOYSA-N cis-caffeic acid Natural products OC(=O)C=CC1=CC=C(O)C(O)=C1 QAIPRVGONGVQAS-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- QGBSISYHAICWAH-UHFFFAOYSA-N dicyandiamide Chemical compound NC(N)=NC#N QGBSISYHAICWAH-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- ILEDWLMCKZNDJK-UHFFFAOYSA-N esculetin Chemical compound C1=CC(=O)OC2=C1C=C(O)C(O)=C2 ILEDWLMCKZNDJK-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- HAVWRBANWNTOJX-UHFFFAOYSA-N fraxetin Chemical compound C1=CC(=O)OC2=C1C=C(OC)C(O)=C2O HAVWRBANWNTOJX-UHFFFAOYSA-N 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- XOXYHGOIRWABTC-UHFFFAOYSA-N gentisin Chemical compound C1=C(O)C=C2C(=O)C3=C(O)C=C(OC)C=C3OC2=C1 XOXYHGOIRWABTC-UHFFFAOYSA-N 0.000 description 1
- ZFLXCZJBYSPSKU-YYSSDTNZSA-N glucoraphenin Natural products C[S@](=O)C=CCCC(=NOS(=O)(=O)O)S[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O ZFLXCZJBYSPSKU-YYSSDTNZSA-N 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 1
- WBAVLTNIRYDCPM-UHFFFAOYSA-N isoscopolin Natural products COC1=CC=2OC(=O)C=CC=2C=C1OC1OC(CO)C(O)C(O)C1O WBAVLTNIRYDCPM-UHFFFAOYSA-N 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- MQYXUWHLBZFQQO-QGTGJCAVSA-N lupeol Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C)CC[C@@H](C(=C)C)[C@@H]5[C@H]4CC[C@@H]3[C@]21C MQYXUWHLBZFQQO-QGTGJCAVSA-N 0.000 description 1
- PKGKOZOYXQMJNG-UHFFFAOYSA-N lupeol Natural products CC(=C)C1CC2C(C)(CCC3C4(C)CCC5C(C)(C)C(O)CCC5(C)C4CCC23C)C1 PKGKOZOYXQMJNG-UHFFFAOYSA-N 0.000 description 1
- IQPNAANSBPBGFQ-UHFFFAOYSA-N luteolin Chemical compound C=1C(O)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C(O)=C1 IQPNAANSBPBGFQ-UHFFFAOYSA-N 0.000 description 1
- LRDGATPGVJTWLJ-UHFFFAOYSA-N luteolin Natural products OC1=CC(O)=CC(C=2OC3=CC(O)=CC(O)=C3C(=O)C=2)=C1 LRDGATPGVJTWLJ-UHFFFAOYSA-N 0.000 description 1
- 235000009498 luteolin Nutrition 0.000 description 1
- 229940117954 naringenin Drugs 0.000 description 1
- WGEYAGZBLYNDFV-UHFFFAOYSA-N naringenin Natural products C1(=O)C2=C(O)C=C(O)C=C2OC(C1)C1=CC=C(CC1)O WGEYAGZBLYNDFV-UHFFFAOYSA-N 0.000 description 1
- 235000007625 naringenin Nutrition 0.000 description 1
- 229940100243 oleanolic acid Drugs 0.000 description 1
- HZLWUYJLOIAQFC-UHFFFAOYSA-N prosapogenin PS-A Natural products C12CC(C)(C)CCC2(C(O)=O)CCC(C2(CCC3C4(C)C)C)(C)C1=CCC2C3(C)CCC4OC1OCC(O)C(O)C1O HZLWUYJLOIAQFC-UHFFFAOYSA-N 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- SGTCGCCQZOUMJJ-YMILTQATSA-N scopolin Chemical compound COC1=CC=2C=CC(=O)OC=2C=C1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O SGTCGCCQZOUMJJ-YMILTQATSA-N 0.000 description 1
- SGTCGCCQZOUMJJ-UHFFFAOYSA-N scopolin Natural products COC1=CC=2C=CC(=O)OC=2C=C1OC1OC(CO)C(O)C(O)C1O SGTCGCCQZOUMJJ-UHFFFAOYSA-N 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- SGEWCQFRYRRZDC-VPRICQMDSA-N vitexin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=C(O)C2=C1OC(C=1C=CC(O)=CC=1)=CC2=O SGEWCQFRYRRZDC-VPRICQMDSA-N 0.000 description 1
- PZKISQRTNNHUGF-UHFFFAOYSA-N vitexine Natural products OC1C(O)C(O)C(CO)OC1OC1=C(O)C=C(O)C2=C1OC(C=1C=CC(O)=CC=1)=CC2=O PZKISQRTNNHUGF-UHFFFAOYSA-N 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
- G01N2030/047—Standards external
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of measuring methods of mangiferin in common bombax flower, and the present invention also provides a kind of quality assessment methods of common bombax flower, belong to drug tests.The present invention includes the following steps: to take pure mangiferin appropriate, 60% methanol is added, the reference substance solution that every lml contains 20 μ g mangiferins is made；Common bombax flower sample powder 0.5g is taken, 60% methanol 25ml is added, weighed weight is ultrasonically treated 1 hour, lets cool, then weighed weight, the weight of less loss is supplied with 60% methanol, is shaken up, and filters, takes subsequent filtrate, obtain solution to be detected；Reference substance solution and each 10 μ l of solution to be detected are drawn respectively, liquid chromatograph are injected, by content assaying method to get mangiferin content.The present invention obtains content in common bombax flower by the chromatographic peak of reference substance solution and the chromatographic peak comparing calculation of solution to be detected, convenient to detect to the content of the mangiferin in common bombax flower.The present invention can also effectively evaluate and test the quality of common bombax flower.
Description
Technical field
The present invention relates to drug test related fields, the measuring method and matter of mangiferin in specifically a kind of common bombax flower
Measure evaluating method.
Background technique
Common bombax flower is a kind of fallen leaves megaphanerophyte, and 25 meters of Gao Keda, bark canescence, the trunk of treelet usually has coniform
Thick thorn；Branch is open and flat.Common bombax flower has very high medical value, is Chinese medicine and Tibetan medicine common medicine.Current standard " Chinese Pharmacopoeia "
Version one character, micro-, tlc identification method and leaching items for only having recorded common bombax flower in 2015, wherein thin-layer chromatography reflects
Other method reference material is common bombax flower control medicinal material, and chemical component qualitatively or quantitatively determines method in uncharged common bombax flower." middle Chinese
People republic the Sanitation Ministry medicine standard " character of common bombax flower (Tibetan language name: receiving loud, high-pitched sound lattice Sa) has only been recorded in Tibetan medicine first.It is existing
Project is qualitatively or quantitatively determined without common bombax flower chemical component in standard, can not evaluate the quality good or not of common bombax flower, common bombax flower
Quality research be badly in need of increase common bombax flower method for quantitatively determining.
Through consulting literatures, the existing research of common bombax flower is less, including in the research of common bombax flower micromorphology, common bombax flower
GC-MS research, the common bombax flower chemical constitution study pharmacology correlative study of volatile oil component.Without any quantitative determination Research Literature
Report.It can see from existing literature, it has been found that contain gallic acid, Quercetin, scopolactone, mangiferin in common bombax flower
(C19H18O11), aurantiamarin, caffeic acid, oleanolic acid, fraxetin, aesculin, chlorogenic acid, Vitexin, luteolin, spun gold
Peach glycosides, protocatechuic acid, gentianic acid, palmitinic acid, lauric acid, scopolin, aesculetin, aesculin, lupeol, feather fan ketone, Hu
The chemical components such as glucoraphenin, succinic acid, naringenin also contain several unknown compounds.
Based on this, for the above existing chemical constitution study, the present invention proposes a kind of measurement of mangiferin in common bombax flower
Method.
Summary of the invention
The purpose of the present invention is to provide a kind of measuring methods of mangiferin in common bombax flower, for detecting the awns in common bombax flower
Fruit glycosides content.
The present invention also provides a kind of quality assessment methods of common bombax flower, detect mango by using above-mentioned measuring method
Glycosides content, can be with the quality of effective evaluation common bombax flower.
The technical solution adopted by the present invention to solve the technical problems is:
The measuring method of mangiferin, includes the following steps: in a kind of common bombax flower
It takes pure mangiferin appropriate, 60% methanol is added, the reference substance solution that every lml contains 20 μ g mangiferins is made；
Common bombax flower sample powder 0.5g is taken, 60% methanol 25ml is added, weighed weight is ultrasonically treated 1 hour, lets cool, then
Weighed weight is supplied the weight of less loss with 60% methanol, is shaken up, and filtration takes subsequent filtrate, obtains solution to be detected；
Reference substance solution and each 10 μ l of solution to be detected are drawn respectively, inject liquid chromatograph, by content assaying method,
Up to mangiferin content.
More preferably, when hplc determination, using octadecylsilane chemically bonded silica as filler；It is 1:9 with volume ratio
- 0.1% phosphoric acid solution of acetonitrile be mobile phase；Detection wavelength is 258nm, and theoretical cam curve is not less than by the calculating of mangiferin peak
3000。
More preferably, sonification power 200W, frequency 40kHz.
More preferably, the common bombax flower sample powder is screened before preparing solution to be detected through No. four.
A kind of quality assessment method of common bombax flower, using any of the above-described one measuring method.
The beneficial effects of the present invention are:
The measuring method of mangiferin in a kind of common bombax flower provided by the invention, can effectively improve mango using 60% methanol
Glycosides (C19H18O11) recovery rate, solution to be detected and reference substance solution are detected by liquid chromatograph, by containing measure
Determine method and obtains content in common bombax flower that is, by the chromatographic peak comparing calculation of the chromatographic peak of reference substance solution and solution to be detected, side
Just the content of the mangiferin in common bombax flower is detected.
The present invention also provides a kind of quality assessment method of common bombax flower, the nature and flavor and channel tropism of common bombax flower: sweet, light, cool.Return
Large intestine channel；Function and the major functions: clearing heat and promoting diuresis, removing toxic substances.For diarrhea, dysentery, hemorrhoid hemorrhage.Modern research suggests that common bombax flower has
The effects of analgesia, anti-inflammatory, anticancer and AntiHIV1 RT activity, anti-oxidant, liver protection, diuresis, disease of cardiovascular system, decompression, hypoglycemic, antibacterial.
And mangiferin has anti-inflammatory, antitumor, anti-oxidant, neuroprotection, brings down a fever, eases pain, the pharmacological actions such as anti parasitic.It can be seen that
The pharmacological action of common bombax flower and mangiferin have important relevance, and mangiferin is the active chemical that common bombax flower plays drug effect,
The superiority and inferiority that the quantitative target for using mangiferin as common bombax flower is used to evaluate common bombax flower quality has reasonability.Therefore pass through above-mentioned survey
The method of determining can effectively detect the quality of common bombax flower.
Detailed description of the invention
Fig. 1 is common bombax flower finger-print (258nm) figure；
Fig. 2 is mangiferin full wavelength scanner figure；
Fig. 3 is the HPLC chromatogram of the reference substance of mangiferin；
Fig. 4 is the HPLC chromatogram of solution to be detected；
Fig. 5 is mangiferin testing result linear dependence figure.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The nature and flavor and channel tropism of common bombax flower: sweet, light, cool.Return large intestine channel；Function and the major functions: clearing heat and promoting diuresis, removing toxic substances.For letting out
It rushes down, dysentery, hemorrhoid hemorrhage.Modern research suggests that common bombax flower has analgesia, anti-inflammatory, anticancer and AntiHIV1 RT activity, anti-oxidant, liver protection, benefit
The effects of urine, disease of cardiovascular system, decompression, hypoglycemic, antibacterial.Anti-inflammatory, the antitumor, antioxygen through having for Literature Consult mangiferin
Change neuroprotection, brings down a fever, eases pain, the pharmacological actions such as anti parasitic.It can be seen that the pharmacological action of common bombax flower has with mangiferin
Important relevance, mangiferin are therefore the active chemical of common bombax flower performance drug effect uses mangiferin quantifying as common bombax flower
The superiority and inferiority that index is used to evaluate common bombax flower quality has reasonability.
It is prepared using different dicyandiamide solution extractions common bombax flower (methanol, ethyl alcohol, water, first alcohol and water, second alcohol and water) to be checked
It surveys solution and the HPLC chromatogram of solution to be detected is obtained using high performance liquid chromatography (i.e. HPLC method), it can with same method
To obtain the HPLC chromatogram of mangiferin reference substance solution, by comparing it can be seen that common bombax flower sample solution is in 200~400nm
Different wave length in resulting chromatogram, have the highest chemical component of chromatographic peak always, the confirmed ingredient is mango
Glycosides.
Embodiment:
1, determination of the mangiferin as target component
It is washed respectively with different flow visualizing (- 0.1% phosphoric acid solution of acetonitrile, -0.1% phosphoric acid solution of methanol) gradients
De-, different solvent extraction sample, investigates chromatogram information under 258nm, 270nm, 310nm and 360nm respectively, and results sample exists
In the finger-print formed under above-mentioned various chromatographic conditions, mangiferin chromatographic peak is top, is shown in the method investigated
In, mangiferin content highest in common bombax flower, so that it is determined that common bombax flower is measurement ingredient.Chromatogram information is most rich at 258nm
Richness selects 258nm to establish finger-print, as shown in Figure 1.17.585min chromatographic peak is mangiferin peak, peak area in finger-print
Integrated value is maximum.
2, chromatographic condition
Chromatographic column: Agilent ZORBAX SB-C18 column (4.6mm × 250mm, 5 μm)；
Column temperature: 35 DEG C；
Mobile phase: acetonitrile-phosphoric acid solution (10:90)；
Flow velocity: 1.0mLmin-1；
Detection wavelength: 258nm；
10 μ L of reference substance sample volume, 10 μ L of sample injection volume；
Number of theoretical plate is calculated by mangiferin peak is not less than 32000.
3, prepared by reference substance solution
Take mangiferin reference substance appropriate, it is accurately weighed, add 60% methanol be made every l ml containing 20 μ g solution to get.
4, solution preparation to be detected
Common bombax flower medicinal powder (needing No. four pharmacopeia sieves) about 0.5g is taken, it is accurately weighed, it sets in stuffed conical flask, precision adds
Enter 60% methanol 25ml, weighed weight, ultrasonic treatment (power 200W, frequency 40kHz) 1 hour is let cool, then weighed weight, is used
60% methanol supplies the weight of less loss, shakes up, filtration, take subsequent filtrate to get.
5, measuring method
Precision weighs above-mentioned for solution to be detected, reference substance solution injection liquid chromatograph, measurement respectively.
6, the selection of wavelength is measured
Full wavelength scanner is carried out to mangiferin reference substance chromatographic peak, as shown in Fig. 2, mangiferin has maximum suction at 258nm
Wavelength is received, it is thus determined that absorbing wavelength is 258nm.Reference substance chromatogram is as shown in figure 3, solution chromatogram such as Fig. 4 to be detected institute
Show.
7, the selection of Extraction solvent
Extracted respectively with a batch common bombax flower medicinal material using different Extraction solvents, be respectively adopted ethyl alcohol, Diluted Alcohol, methanol,
90% methanol, 70% methanol, 60% methanol, 50% methanol prepare sample solution, are measured, and as shown in table 1, as a result selection mentions
When to take solvent be 60% methanol, the integrating peak areas value highest (being indicated in table with A mangiferin) of mangiferin chromatographic peak in common bombax flower,
The recovery rate highest of mangiferin i.e. in common bombax flower.
Table 1
| Extraction solvent | Ethyl alcohol | Diluted Alcohol | Methanol | 90% methanol | 70% methanol | 60% methanol | 50% methanol |
| AMangiferin | 809 | 1003 | 1014 | 1186 | 1252 | 1610 | 1344 |
8, the selection of extracting method and time
With a batch common bombax flower medicinal material, sample solution is prepared using heating reflux method and ultrasonic extraction respectively, such as 2 institute of table
Show, ultrasonic time has investigated 30min, 60min, 90min, and ultrasound and the extraction result for being heated to reflux two methods mangiferin are basic
Unanimously, since both modes can achieve similar effect, ultrasonic extraction is convenient, therefore uses easily operated ultrasound
Method selects the ultrasonic extraction time for 60min.
Table 2
9, linear relationship is investigated
Precision draws mangiferin reference substance solution (concentration 0.0251mg/ml) 2,4,6,10,12,16 μ L, respectively in chromatography
Under the conditions of measure, with peak area (A) to sample volume (μ g) carry out linear regression, obtain regression equation are as follows: y=300.68x-
118.37 r=0.996 6.As shown in table 3 and Fig. 5, the results showed that model of the mangiferin sample volume in 0.0502~0.4016mg
There is good linear relationship in enclosing.
Table 3
10, precision test
Precision absorption mangiferin reference substance solution, continuous sample introduction 6 times.As shown in table 4, its peak area is recorded, mango is measured
The RSD of glycosides peak area is 0.47%, illustrates that the precision of instrument is good.
Table 4
| 1 | 2 | 3 | 4 | 5 | 6 | RSD |
| 895.33 | 901.24 | 896.33 | 904.23 | 893.22 | 895.54 | 0.47% |
11, stability test
It takes and supplies solution to be detected with a, at room temperature respectively in 0,4,8,12, sample introduction measurement for 24 hours, mangiferin average content
RSD be 1.18%.As shown in table 5, the results showed that stablize for solution to be detected is interior for 24 hours at room temperature.
Table 5
| 0 | 4h | 8h | 10h | 12h | 24h | RSD |
| 1260.11 | 1254.24 | 1236.22 | 1246.33 | 1266.78 | 1278.21 | 1.18% |
12, repetitive test
6 parts of same sample lots are taken, every part of about 0.5g is accurately weighed, it is prepared respectively by for solution manufacturing method to be detected,
Sample introduction measures peak area respectively, calculates its content.The average content of mangiferin is 0.16%, RSD 1.77%.As shown in table 6,
The result shows that: this method repeatability is good.
Table 6
| 1 | 2 | 3 | 4 | 5 | 6 | Average (%) | RSD |
| 0.162 | 0.161 | 0.158 | 0.162 | 0.165 | 0.166 | 0.16 | 1.77% |
13, sample recovery rate is tested
Precision weighs 6 parts of same batch of sample (every part of about 0.25g), is separately added into the mangiferin that concentration is 0.2010mg/ml
Reference substance solution 2ml is prepared by solution manufacturing method to be detected, is measured by chromatographic condition, records its peak area.Such as table 7
Shown, the average recovery rate of mangiferin is 99.9%, RSD 0.9%.
Table 7
14, sample measures
By be collected into 19 batches of common bombax flower medicinal materials, respectively by " preparing for solution to be detected " method preparation, peak area is measured,
Its content is calculated, as shown in table 8.
Table 8
Measuring 19 batches of common bombax flower sample size value ranges is 0.05%~0.25%, average value 0.14%, according to country
Pharmacopeia can issue " " Chinese Pharmacopoeia " quality standards in Chinese drugs studies and defines technical requirements ", and assay limit is pressed under its average value
Floating 20%, temporarily ordering limit is that " this product is calculated by dry product, (the C containing mangiferin19H18O11) 0.11% " must not be less than.According to drafting
Limit has 5 batches of sample assay results not meet limit regulation.
From above-described embodiment as can be seen that the quantitative target that the present invention uses mangiferin as common bombax flower is used to evaluate common bombax flower
The superiority and inferiority of quality has reasonability, determines the common bombax flower medicinal material of different sources, different batches, method is reliable, can be used for evaluating
The quality good or not of common bombax flower.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (5)
1. the measuring method of mangiferin in a kind of common bombax flower, which comprises the steps of:
It takes pure mangiferin appropriate, 60% methanol is added, the reference substance solution that every 1ml contains 20 μ g mangiferins is made；
Common bombax flower sample powder 0.5g is taken, 60% methanol 25ml is added, weighed weight is ultrasonically treated 1 hour, lets cool, then weighed
Weight is supplied the weight of less loss with 60% methanol, is shaken up, and filtration takes subsequent filtrate, obtains solution to be detected；
Draw reference substance solution and each 10 μ l of solution to be detected respectively, inject liquid chromatograph, by content assaying method to get
Mangiferin content.
2. the measuring method of mangiferin in a kind of common bombax flower according to claim 1, which is characterized in that liquid chromatograph is surveyed
Periodically, using octadecylsilane chemically bonded silica as filler；Using volume ratio for 1:9-0.1% phosphoric acid solution of acetonitrile as flowing
Phase；Detection wavelength is 258nm, and theoretical cam curve is calculated by mangiferin peak is not less than 3000.
3. the measuring method of mangiferin in a kind of common bombax flower according to claim 1 or 2, which is characterized in that ultrasonic treatment
Power is 200W, frequency 40kHz.
4. the measuring method of mangiferin in a kind of common bombax flower according to claim 3, which is characterized in that the kapok style
Product powder is screened before preparing solution to be detected through No. four.
5. a kind of quality assessment method of common bombax flower, which is characterized in that apply any of the above-described one measuring method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910511187.4A CN110146622A (en) | 2019-06-13 | 2019-06-13 | The measuring method and quality assessment method of mangiferin in a kind of common bombax flower |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910511187.4A CN110146622A (en) | 2019-06-13 | 2019-06-13 | The measuring method and quality assessment method of mangiferin in a kind of common bombax flower |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110146622A true CN110146622A (en) | 2019-08-20 |
Family
ID=67591310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910511187.4A Pending CN110146622A (en) | 2019-06-13 | 2019-06-13 | The measuring method and quality assessment method of mangiferin in a kind of common bombax flower |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110146622A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110780007A (en) * | 2019-12-11 | 2020-02-11 | 广西中医药大学制药厂 | Method for evaluating 6 component contents of mango cough relieving tablet by HPLC (high performance liquid chromatography) method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110046077A1 (en) * | 2008-04-11 | 2011-02-24 | Institut De Recherche Pour Le Developpement (Ird) | Process for the extraction of mangiferin and isomangiferin |
| CN102854283A (en) * | 2012-09-12 | 2013-01-02 | 广州白云山敬修堂药业股份有限公司 | Detection method of polygala arvensis |
| CN105646464A (en) * | 2016-02-23 | 2016-06-08 | 中国药科大学 | Method for extracting high-purity mangiferin from bombax ceiba leaves |
| CN105974014A (en) * | 2016-05-04 | 2016-09-28 | 广州市香雪制药股份有限公司 | Method for detecting rhizome anemarrhenae by quantitative analysis of multi-components by single marker |
| CN109828059A (en) * | 2019-04-02 | 2019-05-31 | 成都中医药大学 | The detection method of Guizhi-Shoyao-Zhimu Decoction |
-
2019
- 2019-06-13 CN CN201910511187.4A patent/CN110146622A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110046077A1 (en) * | 2008-04-11 | 2011-02-24 | Institut De Recherche Pour Le Developpement (Ird) | Process for the extraction of mangiferin and isomangiferin |
| CN102854283A (en) * | 2012-09-12 | 2013-01-02 | 广州白云山敬修堂药业股份有限公司 | Detection method of polygala arvensis |
| CN105646464A (en) * | 2016-02-23 | 2016-06-08 | 中国药科大学 | Method for extracting high-purity mangiferin from bombax ceiba leaves |
| CN105974014A (en) * | 2016-05-04 | 2016-09-28 | 广州市香雪制药股份有限公司 | Method for detecting rhizome anemarrhenae by quantitative analysis of multi-components by single marker |
| CN109828059A (en) * | 2019-04-02 | 2019-05-31 | 成都中医药大学 | The detection method of Guizhi-Shoyao-Zhimu Decoction |
Non-Patent Citations (3)
| Title |
|---|
| XU GUANG-KAI 等: "Antihyperglycemic, antihyperlipidemic and antioxidant effects of standard ethanol extract of Bombax ceiba leaves in high-fat-diet- and streptozotocin-induced Type 2 diabetic rats", 《CHINESE JOURNAL OF NATURAL MEDICINES》 * |
| 周秀萍等: "民族药治疗类风湿性关节炎研究概况", 《中国中药杂志》 * |
| 李明等: "木棉叶化学成分研究", 《中国中药杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110780007A (en) * | 2019-12-11 | 2020-02-11 | 广西中医药大学制药厂 | Method for evaluating 6 component contents of mango cough relieving tablet by HPLC (high performance liquid chromatography) method |
| CN110780007B (en) * | 2019-12-11 | 2022-07-08 | 广西中医药大学制药厂 | Method for evaluating 6 component contents of mango cough relieving tablet by HPLC (high performance liquid chromatography) method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102323371B (en) | Detection method of haemostasis medicine | |
| CN103954724B (en) | Method for detecting Jingfang granules | |
| CN101703611B (en) | Quality detection method of Chinese angelica oral liquid for benefiting blood | |
| CN105628834B (en) | Establishment method and quality evaluation method of Mongolian medicine Louluhua fingerprint | |
| CN104076099A (en) | Method for detection and quality control of medicine composition | |
| CN109342631A (en) | The construction method of the HPLC finger-print of Chinese medicine composition and the quality determining method of Chinese medicine composition | |
| CN109187799A (en) | A kind of fingerprint and its standard finger-print of perilla seed standard decoction | |
| CN102441057B (en) | High performance liquid chromatography (HPLC) fingerprint detection method for blood-nourishing brain-refreshing grain | |
| CN110927311B (en) | Construction method of UPLC (ultra performance liquid chromatography) characteristic spectrum of dogbane leaf medicinal material and method for measuring content of flavonoid component of dogbane leaf medicinal material | |
| CN110146622A (en) | The measuring method and quality assessment method of mangiferin in a kind of common bombax flower | |
| CN105092761B (en) | The detection method of antivirus oral liquid volatile ingredient | |
| CN102359942B (en) | Qingpeng ointment and quality control method of Qingpeng ointment preparation | |
| CN112730674B (en) | Quality detection method of momordica grosvenori tea | |
| CN107389821A (en) | A kind of method of active ingredient in measure ageratum oral liquid | |
| CN100570358C (en) | Treat the detection method of asthenopic pharmaceutical preparation | |
| CN115575541B (en) | Method for simultaneously measuring multiple active ingredients in silver Huang Erchen mixture | |
| CN113156010B (en) | A kind of quality control method of Magnolia Wenzhong decoction material benchmark | |
| CN109900832A (en) | A kind of detection method of rhizoma pinellinae praeparata finger-print | |
| Tyrpenou et al. | Determination of levamisole in sheep muscle tissue by high-performance liquid chromatography and photo diode array detection | |
| CN104597168B (en) | Ramulus Mori refines decoction pieces content assaying method | |
| CN110361459B (en) | Method for establishing loquat leaf standard decoction fingerprint | |
| CN114034798A (en) | A kind of Chishui golden hairpin Dendrobium flower fingerprint construction and content determination method | |
| CN106168608A (en) | The detection method of EGCG content in a kind of tea polyphenols | |
| CN106404935A (en) | Method for detecting EC content in tea polyphenols | |
| CN105158400A (en) | Quality control method of cordate houttuynia eye drop |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190820 |