CN110146366B - A mould for preparing wax stone for pathological tissue embedding - Google Patents
A mould for preparing wax stone for pathological tissue embedding Download PDFInfo
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- CN110146366B CN110146366B CN201910500712.2A CN201910500712A CN110146366B CN 110146366 B CN110146366 B CN 110146366B CN 201910500712 A CN201910500712 A CN 201910500712A CN 110146366 B CN110146366 B CN 110146366B
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- 230000001575 pathological effect Effects 0.000 title claims abstract description 26
- 239000004575 stone Substances 0.000 title claims description 16
- 238000005266 casting Methods 0.000 claims abstract description 18
- 239000000463 material Substances 0.000 claims description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 abstract description 14
- 239000007788 liquid Substances 0.000 abstract description 5
- 230000006378 damage Effects 0.000 abstract description 2
- 230000000149 penetrating effect Effects 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 50
- 239000001993 wax Substances 0.000 description 47
- 239000012188 paraffin wax Substances 0.000 description 18
- 238000000034 method Methods 0.000 description 5
- 241000287828 Gallus gallus Species 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000036285 pathological change Effects 0.000 description 2
- 231100000915 pathological change Toxicity 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 230000000451 tissue damage Effects 0.000 description 2
- 231100000827 tissue damage Toxicity 0.000 description 2
- 210000001534 vitelline membrane Anatomy 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000252212 Danio rerio Species 0.000 description 1
- 241000269368 Xenopus laevis Species 0.000 description 1
- 210000001643 allantois Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000003837 chick embryo Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 210000000713 mesentery Anatomy 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 210000001694 thigh bone Anatomy 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/366—Moulds; Demoulding
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
本发明涉及一种用于制备病理组织包埋用蜡块的模具，包括模框及模框内部的模芯，在模芯的上表面设有若干个蜡块凹槽，在每个蜡块凹槽底部的中心设有竖向贯通模芯的导向孔，在每个导向孔内设有与之匹配且竖向贯穿的顶杆，每根顶杆的上端固定有水平的顶板，在每根顶杆的下部均套装有弹簧，在每个顶板的上表面的中心固定有竖直向上伸出的铸件，本发明制备出的蜡块形态结构完整且带有中央孔隙，使用时可将细小的组织或较薄的组织放入孔隙中，再将蜡液填充孔隙内进行包埋，孔隙可限制组织的移动并防止其倾倒，起到固定组织朝向的作用，便于使用者切片时切到理想部位，同时可有效避免因用镊子反复夹取组织调整其朝向时对组织造成的机械性损伤。
The invention relates to a mold for preparing a wax block for embedding pathological tissue, comprising a mold frame and a mold core inside the mold frame, a plurality of wax block grooves are arranged on the upper surface of the mold core, and each wax block recess The center of the bottom of the groove is provided with a guide hole vertically penetrating the mold core, and each guide hole is provided with a matching ejector pin that penetrates vertically. The upper end of each ejector pin is fixed with a horizontal top plate. The lower parts of the rods are sheathed with springs, and the center of the upper surface of each top plate is fixed with a casting that extends vertically upward. Or thinner tissue is put into the pores, and then the wax liquid is filled into the pores for embedding. The pores can limit the movement of the tissue and prevent it from falling, and play the role of fixing the orientation of the tissue, which is convenient for users to cut to the ideal part when slicing. At the same time, it can effectively avoid the mechanical damage to the tissue caused by repeatedly grasping the tissue with tweezers to adjust its orientation.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a mold for preparing a wax block for embedding fine or thin pathological tissues.
Background
Histopathological examination is an examination method based on microscopic section preparation technology, and because the tissue texture of an organism is soft, efficient section is difficult to perform and ultrathin sections meeting the microscopic examination are difficult to obtain, the preparation of sections after filling tissue cell structures with embedding agents such as paraffin and the like which have a supporting function is needed, and the sections are called paraffin sections. For paraffin embedding, a method is generally adopted in which molten paraffin is poured into an embedding frame, a tissue block which is subjected to the steps of transparence and wax immersion is clamped by forceps before paraffin is solidified and placed into the molten paraffin, the direction of the tissue block is adjusted by the forceps so as to be cut to an ideal position during slicing, and after the direction of the tissue block is adjusted, the paraffin block is condensed at room temperature or placed into cold water to accelerate the solidification of the paraffin block; and after the wax block is solidified, removing the embedding frame and taking out the wax block for subsequent slicing operation. There are at least three factors that affect the quality and efficiency of tissue embedding during this process:
firstly, the position or the direction of a tissue block needs to be repeatedly moved when the embedding orientation of the tissue block is adjusted, paraffin is quickly solidified at room temperature, the phenomenon that the orientation of the tissue block is not adjusted and paraffin liquid is solidified can occur, and a section cannot be cut to an ideal position; secondly, even if the orientation can be adjusted before paraffin is solidified, due to the lack of auxiliary support of the tissue block in the molten paraffin, the tissue block is easy to shift or topple before the paraffin is solidified, especially for some small tube-cavity tissues such as mouse bones or sheet tissues such as chick embryo vitelline membrane and the like; thirdly, when the position or the direction of the tissue block is adjusted by the forceps, the tissue needs to be repeatedly clamped, mechanical damage to the tissue can be caused, and subsequent observation and judgment of the tissue lesion can be possibly influenced.
Disclosure of Invention
In view of the above problems in the tissue embedding process, the present invention provides a mold for preparing a wax block for pathological tissue embedding.
The specific contents are as follows: the utility model provides a mould for preparing wax stone for pathological tissue embedding, this mould includes the framed and installs the inside mold core at the framed, characterized by:
the die frame is a cuboid frame body, the die core is matched with the die frame and is arranged in the die frame, a bottom plate is fixed at the bottom of the die frame, a circle of convex edge extending horizontally inwards is arranged on the upper edge of the inner side surface of the die frame, a vertical guide rail is arranged on the side surface of the die core, a vertical sliding groove matched with the guide rail is arranged on the inner side surface of the die frame, and the die core is matched with the sliding groove on the die frame through the guide rail so that the die core forms a vertical sliding pair in the die frame;
the upper surface of mold core be equipped with a plurality of wax stone recess, be equipped with the vertical guiding hole that link up the mold core at the center of every wax stone recess bottom, be equipped with the ejector pin that matches and vertically run through with it in every guiding hole, the upper surface at the bottom plate is fixed to the lower extreme of every ejector pin, the upper end of every ejector pin is fixed with the horizontally roof, every roof corresponds rather than the bottom of the wax stone recess that corresponds and matches, all the cover is equipped with the spring in the lower part of every ejector pin, the upper end of this spring is supported in the bottom of mold core, the lower extreme of spring supports the upper surface at the bottom plate, and the mold core passes through the bottom support of wax stone recess on the lower surface of roof, be fixed with the vertical foundry goods that upwards stretch out at the center of every roof upper surface, and the upper end of every foundry goods all stretches out the upper surface of mold core.
Preferably, the casting is cylindrical or straight quadrangular.
Preferably, the wax block groove is in an inverted regular quadrangular frustum pyramid shape.
Preferably, the bottom plate of the mold frame is fixed on the bottom surface of the mold frame through screws.
Preferably, the lower end of each ejector rod is fixed with the bottom plate through a screw.
Preferably, the die frame, the bottom plate, the die core, the ejector rod, the top plate and the casting are all made of stainless steel materials or copper materials.
The invention has the beneficial technical effects that:
the invention relates to a mould for preparing wax block for embedding pathological tissues, which comprises a mould frame and a mould core inside the mould frame, wherein a plurality of wax block grooves are arranged on the upper surface of the mould core, a guide hole vertically penetrating through the mould core is arranged at the center of the bottom of each wax block groove, a push rod which is matched with and vertically penetrates through each guide hole is arranged in each guide hole, a horizontal top plate is fixed at the upper end of each push rod, a spring is sleeved at the lower part of each push rod, and a casting which vertically extends upwards is fixed at the center of the upper surface of each top plate. Orientation when the existence of central authorities hole can effectively control the tissue piece embedding avoids getting the tissue with tweezers repeatedly clamp, has so also avoided the tissue damage that tweezers caused and influence observation and the judgement of section pathological change, can select the wax piece that the foundry goods that corresponds shape and size poured into a mould and formed simultaneously according to the shape and the size of pathological tissue, makes the shape of hole in the wax piece match with the shape of pathological tissue as far as.
Drawings
FIG. 1 is a schematic perspective view of a mold for preparing a wax block for pathological tissue embedding;
FIG. 2 is a schematic cross-sectional view of FIG. 1;
FIG. 3 is a schematic perspective view of a mold core;
FIG. 4 is a schematic perspective view of a mold frame;
FIG. 5 shows a wax block cast from the mold of the present invention (a cylindrical casting is used as an example);
FIG. 6 is a schematic illustration of the specific operation of one embodiment of the present invention (core pressed down, wax block removed);
in the figure: 1. the mould comprises a mould frame, 11 convex edges, 12 sliding grooves, 13 bottom parts of the mould frame, 2 mould cores, 21 wax block grooves, 22 guide holes, 23 guide rails, 3 bottom plates, 31 screws, 41 castings, 42 top plates, 43 ejector rods, 44 springs, 5 wax blocks and 51 central holes.
Detailed Description
In a first embodiment, referring to fig. 1-6, a mold for preparing a wax block for embedding pathological tissues comprises a mold frame and a mold core arranged in the mold frame;
the die frame is a cuboid frame body, the die core is matched with the die frame and is arranged in the die frame, a bottom plate is fixed at the bottom of the die frame, a circle of convex edge extending horizontally inwards is arranged on the upper edge of the inner side surface of the die frame, a vertical guide rail is arranged on the side surface of the die core, a vertical sliding groove matched with the guide rail is arranged on the inner side surface of the die frame, and the die core is matched with the upper sliding groove of the die frame through the guide rail so that the die core forms a vertical sliding pair in the die frame;
the upper surface of mold core be equipped with a plurality of wax stone recess, be equipped with the vertical guiding hole that link up the mold core at the center of every wax stone recess bottom, be equipped with the ejector pin that matches and vertically run through with it in every guiding hole, the upper surface at the bottom plate is fixed to the lower extreme of every ejector pin, the upper end of every ejector pin is fixed with the horizontally roof, every roof corresponds rather than the bottom of the wax stone recess that corresponds and matches, all the cover is equipped with the spring in the lower part of every ejector pin, the upper end of this spring is supported in the bottom of mold core, the lower extreme of spring supports the upper surface at the bottom plate, and the bottom that the mold core passes through the wax stone recess supports the lower surface at the roof, be fixed with the vertical foundry goods that upwards stretch out at the center of every roof upper surface, and the upper end of every foundry goods all stretches out the upper surface of mold core.
The castings are cylindrical or straight quadrangular prisms, and the castings with corresponding shapes, namely cylindrical castings with different diameters or straight quadrangular prism castings with different thicknesses, can be flexibly selected according to the shapes and sizes of tissues to be embedded, so that the shapes of pores in the wax block formed by pouring are matched with the shapes of pathological tissues as much as possible, and the directional embedding of the pathological tissues is facilitated.
The wax block groove is inverted regular quadrangular frustum pyramid-shaped, and a wax block formed by pouring the wax block groove in the shape can be effectively and completely demoulded.
The bottom plate is fixed on the bottom surface of the die frame through screws, so that the die core and other internal parts of the die core can be conveniently detached, installed, maintained and cleaned.
The lower end of each ejector rod is fixed with the bottom plate through screws, and the ejector rods and the top plate can be flexibly disassembled and assembled according to use requirements so as to replace castings in different shapes.
The die frame, the bottom plate, the die core, the ejector rod, the top plate and the casting are all made of stainless steel materials or copper materials.
The mold is mainly used for embedding tiny or thinner pathological tissues, and can be tiny tube-cavity tissues such as mouse thighbone, xenopus laevis bone, zebra fish juvenile fish and the like, and can also be thinner pathological tissues such as sheet chicken embryo yolk membrane, chicken embryo allantoic membrane, mesentery and the like.
The die of the invention has the following use process and principle:
selecting a casting with a corresponding shape according to the shape or size of the tissue during actual embedding, installing the casting on a mold, checking the mold to ensure that the mold core is naturally supported and tightly attached to a top plate under the action of spring elasticity, then pouring molten paraffin into the wax block grooves, wherein the surface of the poured paraffin is not higher than the upper surface of the mold core, selecting the number of the poured wax block grooves according to actual requirements, and after the surface layer of the paraffin is solidified at room temperature after the pouring is finished, putting the whole mold into cold water to accelerate the solidification of the paraffin; after the poured paraffin is completely solidified, slightly pressing the mold core downwards by hands to enable the mold core to gradually move downwards, simultaneously enabling the solidified wax block to be gradually ejected out of a top plate supported by an ejector rod relative to a wax block groove to realize demolding, then slightly lifting the wax block upwards by hands to enable the wax block to be completely separated from a casting to obtain the wax block with a central pore with a specific shape and an intact structure, finally placing the prepared wax block on a platform, placing a tube-cavity tissue or a sheet tissue to be embedded into the central pore, adjusting the position and the angle of a pathological tissue by using tweezers to enable a target area to be sliced in the pathological tissue to be close to the bottom of the pore of the wax block, filling the central pore with wax liquid, and adhering the wax block embedded with the pathological tissue on a bench wood for slicing after the wax liquid is solidified.
The wax block prepared by the invention has a complete shape and structure and pores, when in use, fine tissues or thinner tissues are put into the pores, and then the pores are filled with the wax liquid for embedding, the pores can limit the movement of the tissues and prevent the tissues from toppling over, so that the orientation of the tissues is fixed, a user can conveniently cut an ideal part when slicing, the orientation of the tissue block during embedding can be effectively controlled due to the existence of the central pores, the observation and judgment of the pathological changes of the slices are prevented from being influenced due to the tissue damage possibly caused by repeatedly clamping the tissues by using tweezers, and meanwhile, the wax block can be poured by selecting castings with corresponding shapes and sizes according to the shapes and sizes of pathological tissues, so that the shapes of the pores of the wax block are matched with the shapes of the pathological tissues as far as possible.
Claims (6)
1. The utility model provides a mould for preparing wax stone for pathological tissue embedding, this mould includes the framed and installs the inside mold core at the framed, characterized by:
the die frame is a cuboid frame body, the die core is matched with the die frame and is arranged in the die frame, a bottom plate is fixed at the bottom of the die frame, a circle of convex edge extending horizontally inwards is arranged on the upper edge of the inner side surface of the die frame, a vertical guide rail is arranged on the side surface of the die core, a vertical sliding groove matched with the guide rail is arranged on the inner side surface of the die frame, and the die core is matched with the sliding groove on the die frame through the guide rail so that the die core forms a vertical sliding pair in the die frame;
the upper surface of mold core be equipped with a plurality of wax stone recess, be equipped with the vertical guiding hole that link up the mold core at the center of every wax stone recess bottom, be equipped with the ejector pin that matches and vertically run through with it in every guiding hole, the upper surface at the bottom plate is fixed to the lower extreme of every ejector pin, the upper end of every ejector pin is fixed with the horizontally roof, every roof corresponds rather than the bottom of the wax stone recess that corresponds and matches, all the cover is equipped with the spring in the lower part of every ejector pin, the upper end of this spring is supported in the bottom of mold core, the lower extreme of spring supports the upper surface at the bottom plate, and the mold core passes through the bottom support of wax stone recess on the lower surface of roof, be fixed with the vertical foundry goods that upwards stretch out at the center of every roof upper surface, and the upper end of every foundry goods all stretches out the upper surface of mold core.
2. The mold for preparing a wax block for embedding pathological tissues as set forth in claim 1, wherein: the casting is cylindrical or straight quadrangular.
3. The mold for preparing a wax block for embedding pathological tissues as set forth in claim 1, wherein: the wax block groove is in an inverted regular quadrangular frustum pyramid shape.
4. The mold for preparing a wax block for embedding pathological tissues as set forth in claim 1, wherein: the die frame bottom plate is fixed on the bottom surface of the die frame through screws.
5. The mold for preparing a wax block for embedding pathological tissues as set forth in claim 1, wherein: the lower end of each ejector rod is fixed with the bottom plate through a screw.
6. The mold for preparing a wax block for embedding pathological tissues as set forth in claim 1, wherein: the die frame, the bottom plate, the die core, the ejector rod, the top plate and the casting are all made of stainless steel materials or copper materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910500712.2A CN110146366B (en) | 2019-06-11 | 2019-06-11 | A mould for preparing wax stone for pathological tissue embedding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910500712.2A CN110146366B (en) | 2019-06-11 | 2019-06-11 | A mould for preparing wax stone for pathological tissue embedding |
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| CN110146366A CN110146366A (en) | 2019-08-20 |
| CN110146366B true CN110146366B (en) | 2022-04-05 |
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| CN201910500712.2A Active CN110146366B (en) | 2019-06-11 | 2019-06-11 | A mould for preparing wax stone for pathological tissue embedding |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111889621A (en) * | 2020-08-10 | 2020-11-06 | 马鞍山市兴隆铸造有限公司 | Wax block making equipment for investment machining casting process |
| MX2022015557A (en) * | 2022-12-07 | 2024-06-10 | Karla Maria Munoz Alcocer | Mold for encapsulating samples of millimetric dimensions. |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5665398A (en) * | 1995-11-20 | 1997-09-09 | Mccormick; James B. | Apparatus for embedding tissue samples |
| CN1920558A (en) * | 2005-12-27 | 2007-02-28 | 胡苹 | Wax module for organizing chip array |
| CN202471473U (en) * | 2011-09-14 | 2012-10-03 | 饶莹 | Device for manufacturing tissue chip at one time |
| CN107053543A (en) * | 2017-04-24 | 2017-08-18 | 华中科技大学 | Suitable for anaerobic resin imbedded mold |
| CN207456912U (en) * | 2017-09-26 | 2018-06-05 | 西安培华学院 | A kind of pathologic tissue packing die |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109443897A (en) * | 2018-12-27 | 2019-03-08 | 沈阳安真医疗器械有限公司 | Multifunctional unit tissue embedding box |
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- 2019-06-11 CN CN201910500712.2A patent/CN110146366B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5665398A (en) * | 1995-11-20 | 1997-09-09 | Mccormick; James B. | Apparatus for embedding tissue samples |
| CN1920558A (en) * | 2005-12-27 | 2007-02-28 | 胡苹 | Wax module for organizing chip array |
| CN202471473U (en) * | 2011-09-14 | 2012-10-03 | 饶莹 | Device for manufacturing tissue chip at one time |
| CN107053543A (en) * | 2017-04-24 | 2017-08-18 | 华中科技大学 | Suitable for anaerobic resin imbedded mold |
| CN207456912U (en) * | 2017-09-26 | 2018-06-05 | 西安培华学院 | A kind of pathologic tissue packing die |
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