CN110106117A - Regional efficient nitrogen microbial composite bacteria agent and its application in a kind of suitable Guizhou Province - Google Patents

Abstract

The invention belongs to the technical field of microbial compound bacterial agents, and particularly relates to a high-efficiency nitrogen conversion microbial compound bacterial agent suitable for central Guizhou and its application, consisting of bacterial strains Naxibacter sp. Composition with an effective bacterial count ratio of 1:1:0.8‑1.2:1:0.8‑1.2. The microbial compound bacterial agent prepared by the invention has strong ammonification ability, is beneficial to efficient transformation of soil organic nitrogen into ammonium nitrogen, and is especially suitable for central Guizhou regions.

Description

A high-efficiency nitrogen conversion microbial compound bacterial agent suitable for central Guizhou and its application

technical field

The invention belongs to the technical field of microbial compound bacterial agents, in particular to a high-efficiency nitrogen conversion microbial compound bacterial agent suitable for central Guizhou and its application.

Background technique

Nitrogen fertilizer is an important guarantee for the construction of crop yield and quality. The nitrogen in crops mainly comes from the soil. The nitrogen in the soil is mainly organic nitrogen, accounting for more than 85%, and the proportion of inorganic nitrogen is relatively small. The nitrogen absorbed by crops during the growth process is mainly inorganic nitrogen converted from organic nitrogen in the soil, rather than chemical fertilizers (inorganic nitrogen) applied directly in the season. Different crops have large differences in nitrogen demand and demand time. For example, cabbage has a large demand for nitrogen throughout the growth period; corn, rice, etc. will appear green and late due to excessive nitrogen supply; In the early stage of growth, the demand for nitrogen is more, and the supply of nitrogen is required to be reduced in the later stage. Therefore, adjusting the conversion time and rate of nitrogen in the soil according to the characteristics of crop fertilizer requirements is conducive to improving fertilizer use efficiency, and at the same time achieving high-quality, high-yield, and safe production of crops.

Under natural conditions, the transformation of soil organic nitrogen mainly relies on ammonifying bacteria, nitrifying bacteria, and nitrosifying bacteria in the soil, and the transformation is relatively slow. Ammonifying bacteria convert amino acids into ammonium nitrogen, and ammonium nitrogen converts ammonium nitrogen into nitrate nitrogen under the action of nitrifying bacteria (including nitrosifying bacteria and nitrifying bacteria). Both ammonium nitrogen and nitrate nitrogen are inorganic nitrogen, which are the main forms of nitrogen absorbed by plants. There are many nitrogen-transforming microorganisms, and the ones with high conversion efficiency can be selected for application, which can improve the nitrogen conversion efficiency.

Contents of the invention

The microbial compound bacterial agent prepared by the invention has strong ammonification ability, is beneficial to efficient transformation of soil organic nitrogen into ammonium nitrogen, and is especially suitable for central Guizhou regions.

The invention provides a high-efficiency nitrogen conversion microbial compound bacterial agent suitable for the central Guizhou region, which is composed of bacterial strain Naxibacter sp.HMD2167, bacterial strain Massiliabrevitalea, bacterial strain Stenotrophomonas rhizophila, bacterial strain Bacillus pumilus, and bacterial strain Burkholderiacepacia at an effective bacterial number ratio of 1:1:0.8- 1.2:1:0.8-1.2 composition, and the concentration of each strain is ≥1.0×10 ⁸ cells/mL.

Preferably, the above-mentioned high-efficiency nitrogen conversion microbial compound bacterial agent suitable for the central Guizhou region is composed of the strain Naxibactersp. .

The composite bacterial agent is obtained by preparing each bacterial strain into a culture solution, and then formulating according to the ratio of the effective bacterial count.

An application of the above-mentioned high-efficiency nitrogen conversion microbial compound bacterial agent suitable for the central Guizhou region in improving soil ammonium nitrogen.

Compared with prior art, the beneficial effect of the present invention:

The microbial composite bacterial agent prepared by the invention has strong ammonification ability, which is beneficial to the efficient conversion of soil organic nitrogen into ammonium nitrogen, improves the utilization rate of fertilizers, provides guarantee for crop growth, and can also promote the activity of other microorganisms in the original soil ecological environment, and the Bacteria have strong adaptability in central Guizhou, especially in Anshun and its ecologically similar areas.

Description of drawings

Fig. 1 is a diagram showing the influence of different amounts of effective microbial bacterial agents inoculated in Example 2 on ammonium nitrogen in the present invention.

Detailed ways

Several specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. If there is no special description, the liquid culture process and strain screening process are all conventional technical means in the field, but it should be understood that the scope of protection of the present invention is not limited by specific implementation. The limitation of the method, the separation and purification and other operations involved are all conventional methods in the art unless otherwise specified.

Example 1

A high-efficiency nitrogen-converting microbial compound bacterial agent suitable for central Guizhou is composed of strain Naxibactersp.HMD2167 (provided by the Key Laboratory of Tobacco Quality Research, Tobacco College, Guizhou University, No. Provided by the Key Laboratory of Tobacco Quality Research, Tobacco College, University of Tobacco, No. 2017-07; screened from soil in central Guizhou), the strain Stenotrophomonas rhizophila (provided by the Key Laboratory of Tobacco Quality Research, Tobacco College, Guizhou University, No. 2017-08; screened from soil in Central Guizhou screening), strain Bacillus pumilus (provided by the Key Laboratory of Tobacco Quality Research, Guizhou University Tobacco College, No. 10; screened from the soil in central Guizhou) with an effective bacterial count ratio of 1:1:1:1:1, and the concentration of each strain was ≥1.0×10 ⁸ cells/mL. The preparation process of the bacterial agent is to prepare various bacterial strains into a culture solution and then carry out solution proportioning. The existing LB culture solution can be used to activate each bacterial strain at room temperature for 12 hours, and then expand the culture on a shaking table at 30° C. and then prepare in proportion.

The application of the above-mentioned high-efficiency nitrogen conversion microbial compound bacterial agent suitable for the central Guizhou region in improving soil ammonium nitrogen is applied to the soil one week before the maximum nitrogen demand period of crops (such as flue-cured tobacco), and the optimal application rate is 20mL/ strain.

The bacterial agent is used in central Guizhou, especially in Anshun and areas with similar ecological environments to Anshun.

Example 2

The difference between the composite bacterial agent in Example 2 and Example 1 is that the bacterial strain Naxibacter sp.HMD2167, the bacterial strain Massiliabrevitalea, the bacterial strain Stenotrophomonas rhizophila, the bacterial strain Bacillus pumilus, and the bacterial strain Burkholderiacepacia are composed of an effective bacterial number ratio of 1:1:0.8:1:0.8, and the rest The steps are all the same, and the effect of repressing is slightly inferior to the compound bacterial agent in implementation 1.

Example 3

The difference between the composite microbial agent in Example 3 and Example 1 is that the bacterial strain Naxibacter sp.HMD2167, the bacterial strain Massiliabrevitalea, the bacterial strain Stenotrophomonas rhizophila, the bacterial strain Bacillus pumilus, and the bacterial strain Burkholderiacepacia are composed of an effective bacterial number ratio of 1:1:1.2:1:1.2, and the rest The steps are all the same, and the retransfer effect is slightly inferior to that of the compound microbial agent in Implementation 1, and is equivalent to that of Example 2.

Before the experiment, we isolated and purified the strains with ammonification ability from tobacco planting soil in different areas of Anshun, and compared the ammonification intensity of different strains. The specific process was as follows: inoculate different strains in LB liquid medium, After being activated for 12 hours, 4% inoculum was added to 50 mL (in a 250 mL Erlenmeyer flask) beef extract peptone culture solution, and three replicates were set up for each strain. Cultivate in a constant temperature shaker (130r/min, 30°C) according to the separation conditions. Samples were taken at 0h, 12h, 24h, 48h, and 72h to measure the intensity of ammoniation. The results are shown in Table 1: the strains with the strongest ammonification were Naxibactersp.HMD2167, Massiliabrevitalea, Stenotrophomonas rhizophila, Bacillus pumilus and Burkholderia cepacia.

Table 1 Changes of ammoniation intensity of different strains at different sampling times

Application amount comparison: use straw and chicken manure as organic fertilizer, apply it to the soil, apply the bacterial agent 0mL/strain, 2mL/strain, 10mL/strain, and 20mL/strain of Example 2 respectively, and the concentration of the bacterial solution is ≥1.0×10 ⁸ /mL, see Table 2.

Table 2 Experimental program design

The results are shown in Figure 1. From Figure 1, it can be seen that the ammonium nitrogen content of straw and chicken manure showed an upward trend during the 5-30d composting process. Among them, the largest change was in treatment 1, 95.8% higher in 30d than 5d; the smallest change was in treatment 5, 19.3% higher in 30d than 5d. Treatment 2 was lower than treatment 1 at 15 days, and the ammonium nitrogen content of straw composting treated with microbial inoculum was higher than that of treatment without microbial inoculum at other times, and the content of ammonium nitrogen increased with the increase of inoculum application amount .

It should be noted that the steps and methods adopted in the claims of the present invention are the same as those of the above-mentioned embodiments. In order to avoid repetition, the present invention has described preferred embodiments, but once those skilled in the art know the basic creative concept, Additional changes and modifications can then be made to these embodiments. Therefore, it is intended that the appended claims be construed to cover the preferred embodiment as well as all changes and modifications which fall within the scope of the invention.

Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention also intends to include these modifications and variations.

Claims (4)

1. A kind of high-efficiency nitrogen transformation microbial composite bacterial agent suitable for Guizhou central area is characterized in that, by bacterial strain Naxibactersp.HMD2167, bacterial strain Massiliabrevitalea, bacterial strain Stenotrophomonas rhizophila, bacterial strain Bacillus pumilus, bacterial strain Burkholderiacepacia with effective bacterial count ratio 1:1:0.8 -1.2:1:0.8-1.2 composition, and the concentration of each strain is ≥1.0×10 ⁸ cells/mL. 2. as claimed in claim 1, the high-efficiency nitrogen conversion microbial composite bacterial agent suitable for the central region of Guizhou is characterized in that, by bacterial strain Naxibacter sp. 1:1:1:1:1 composition. 3. as claimed in claim 1, the high-efficiency nitrogen conversion microbial composite bacterial agent suitable for the central Guizhou region is characterized in that, after each bacterial strain is prepared into a culture solution, the composite bacterial agent is then prepared according to 1:1:0.8-1.2 :1:0.8-1.2 The ratio of the effective number of bacteria is obtained after proportioning. 4. the application of the high-efficiency nitrogen conversion microbial composite bacterial agent suitable for the central Guizhou region described in claim 1 in improving soil ammonium nitrogen.