CN110101652A - 一种修护精华露 - Google Patents
一种修护精华露 Download PDFInfo
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- CN110101652A CN110101652A CN201910518204.7A CN201910518204A CN110101652A CN 110101652 A CN110101652 A CN 110101652A CN 201910518204 A CN201910518204 A CN 201910518204A CN 110101652 A CN110101652 A CN 110101652A
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Abstract
本发明提供了一种修护精华露,包括复合植物精粹,所述复合植物精粹主要由小白菊、金黄洋甘菊、母菊经粉碎、浸泡、加入纤维毒酶和乙醇,超声辅助提取得到。所述复合植物精粹具有抑制细胞DNA损伤和泄露,可以提高皮肤免疫力和抵抗力,以应对恶劣环境中对辐射或其他刺激源的损害;所述修护精华露还可以具有对受损皮肤疼痛、干燥、脱皮、瘙痒等症状有良好的修护作用,具有促进皮肤新陈代谢,活血通络,能提升皮肤含水量,使得皮肤更加柔嫩、滋润、有光泽。
Description
技术领域
本发明涉及化妆品领域,具体涉及一种修护精华露。
背景技术
皮肤是覆盖在人体表面的最大器官,健康皮肤的基本要素是光滑、充盈、富有弹性。皮肤直接与外界环境接触,受到氧化、干燥、紫外线等种种因素的刺激,容易受到损伤。同时,随着全球生态环境的不断恶化,加上人们对皮肤健康意识的提高,因肌肤损伤导致的皮肤敏感与刺激的感知率和关注度逐渐上升。据不完全统计,全球自我感觉敏感性肌肤的人群越来越多,其中男性占 38%,女性占 61%。因此,具有修护功效的化妆品具有良好的市场前景。然而,目前市场上出售的专注于修护的护肤品少,且大都效果有限。
发明内容
基于此,本发明的目的在于提供一种修护精华露,可以提高皮肤免疫力和抵抗力,对受损肌肤具有良好的修护作用。
本发明的具体技术方案如下:
一种修护精华露,主要由以下质量百分含量的各组分组成:丁二醇1-5%,玉米谷蛋白氨基酸类 0.5-5%,水解胶原0.5-2.5%,银耳(TREMELLA FUCIFORMIS)提取物0.01-0.2%,泛醇0.1-1%,硅烷三醇1-5%,寡肽-1的1‰水溶液0.1-2%,寡肽-2 的1‰水溶液0.1-2%,寡肽-5的1‰水溶液0.1-2%,神经酰胺3 0.01-0.1%,乙酰基六肽-8的1‰水溶液0.1-1%,叶酸0.01-0.1%,芽孢杆菌/大豆发酵产物提取物0.1-1%,透明质酸钠0.01-0.1%,磷脂0.01-0.1%,茶(CAMELLIA SINENSIS)提取物0.1-2%,木兰醇0.1-2%,薄荷(MENTHA ARVENSIS)叶提取物0.1-2%,马齿苋(PORTULACA OLERACEA)提取物0.1-2%,库拉索芦荟(ALOE BARBADENSIS)提取物0.1-2%,苦参(SOPHORA FLAVESCENS)提取物0.1-1%,黄芩(SCUTELLARIA BAICALENSIS)根提取物0.1-1%,茶(CAMELLIA SINENSIS)叶提取物0.1-1%,光果甘草(GLYCYRRHIZAGLABRA)提取物0.1-1%,迷迭香(ROSMARINUS OFFICINALIS)叶提取物0.1-1%,虎杖(POLYGONUM CUSPIDATUM)根提取物0.1-1%,柚(CITRUS GRANDIS)果提取物0.1-1%,己二醇0.1-1%,复合植物精粹0.1-10%,水45-90%;其中所述的复合植物精粹主要由小白菊、金黄洋甘菊、母菊提取得到。
在其中一些实施例中,所述复合植物精粹主要由以下重量份原料提取得到:小白菊2-8份,金黄洋甘菊1-10份,母菊1-10份。
在其中一些实施例中,所述复合植物精粹由以下重量份原料提取得到:小白菊2-8份,金黄洋甘菊1-10份,母菊1-10份,花椒2-8份,积雪草2-8份。
在其中一些实施例中,所述的复合植物精粹由以下方法制备得到,具体步骤如下:
1)称取干燥后的原料,混合,粉碎得原料粉末;
2)将原料粉末加水浸泡,匀浆,得浆液;
3)浆液调节PH值至5-6,然后加入纤维素酶,加乙醇,超声辅助提取得提取液;
4)提取液过滤,减压浓缩除去乙醇,过滤,离心,得复合植物精粹。
在其中一些实施例中,步骤1)中所述原料粉碎至20-80目。
在其中一些实施例中,步骤2)中所述的浸泡条件为加原料粉末重量2-3倍的水,浸泡1-2小时。
在其中一些实施例中,步骤3)中所述加入纤维素酶的量为浆液重量的1-2wt%;所述乙醇的加入终浓度为65-75%(体积分数);所述超声条件为:频率30-40KHZ,温度为30-55℃,作用时间为20-40min。
在其中一些实施例中,步骤4)中所述减压浓缩条件为在真空度为0.08-0.1Mpa,温度40℃-45℃下减压蒸馏;所述离心为1000-5000rpm离心10-30分钟。
本发明具有如下的优点及有益效果:
(1)本发明的修护精华露是经发明人大量的实验和研究获得的,精选玉米谷蛋白氨基酸类、水解胶原、泛醇、硅烷三醇、寡肽、神经酰胺、乙酰基六肽-8、叶酸、透明质酸钠、复合植物精粹按照特定的比例进行配伍,并得出其最佳配方组成。其中神经酰胺、透明质酸钠可以提升肌肤角质层的水合程度,提升肌肤含水量,维护肌肤屏障;玉米谷蛋白氨基酸类、水解胶原、泛醇、硅烷三醇、寡肽、乙酰基六肽-8具有良好的滋润营养肌肤、增加肌肤活性,提升新陈代谢的作用;复合植物精粹可以提高皮肤免疫力和抵抗力,以应对恶劣环境中对辐射或其他刺激源的损害。
(2)本发明所述复合植物精粹为小白菊、金黄洋甘菊、母菊的混合物提取液,所述提取液的制备方法的工艺参数,经过发明人大量的实验和经验,得到最优的工艺组合,通过该方法制得的提取液中物质的活性较高,其用于化妆品中具有显著的肌肤修护效果,且研究过程中得出,用小白菊、金黄洋甘菊、母菊的混合物所制得提取液,其肌肤修护效果显著优于先将小白菊、金黄洋甘菊、母菊单独提取,后混合所制得提取液的效果。
(3)实践证明,本发明所提供的修护精华露连续使用7天,能提升皮肤含水量,修护改善皮肤皮肤疼痛、干燥、脱皮、瘙痒问题,且本发明产品可长期使用,无毒副作用,刺激性小。
具体实施方式
为使本发明的目的、技术方案和优点更加清楚,下面将对本发明实施方式作进一步地详细描述。但本发明的实施方式不限于此,对于未特别注明的工艺参数或条件,可参照常规技术进行。
本发明所涉及的部分原料简介如下:
本发明所述小白菊别名钮扣菊,是菊科、菊属的二年生草本花卉、多年生草本,拉丁学名:Chrysanthemum parthenium,提取部位是花蕾。小白菊可以用水或酒精等为溶剂提取。小白菊主要含有挥发油(α-蒎烯)、倍半萜内酯(小白菊内酯)、倍半萜(樟脑)等。本发明所制得的小白菊提取物的化妆品中文名称为小白菊(CHRYSANTHEMUM PARTHENIUM)提取物。
本发明所述金黄洋甘菊是菊科植物,别称又叫罗马洋甘菊,拉丁学名为CHRYSANTHELLUM INDICUM,提取部位为花,金黄洋甘菊可以用水或酒精等为溶剂,按常规方法提取。金黄洋甘菊精油的主要化学成分 松萜或蒎烯、莰烯、桧烯、月桂烯、桉树脑、松油精萜品烯、石竹(萜)烯等。本发明所制得的金黄洋甘菊提取物的化妆品中文名称为金黄洋甘菊(CHRYSANTHELLUM INDICUM)提取物。
本发明所述母菊为菊科、母菊属一年生草本植物。拉丁学名为:Matricaria recutita L.,提取部位为头状花序,母菊可以以水或酒精等为溶剂,按常规方法提取。母菊花含胆碱(choline)、芹菜素(api-genin)等,花中粘液质有半乳糖酣酸(galacturonicacid)、半乳糖(galactose)、木糖(xylose)及多糖(polysaccharides)等。本发明所制得的母菊提取物的化妆品中文名称为母菊(CHAMOMILLA RECUTITA)花提取物。
本发明所述花椒是芸香科、花椒属落叶小乔木,拉丁学名为:Zanthoxylum bungeanum Maxim.,提取部位为果实,花椒可以以水或酒精等为溶剂,按常规方法提取。花椒果实含挥发油,主要是4-松油烯醇,其次是辣薄荷酮(piperitone),另有芳樟醇、香桧烯、柠檬烯、邻-聚伞花素(o-cymene)等。本发明所制得的花椒提取物的化妆品中文名称为花椒(ZANTHOXYLUM BUNGEANUM)果提取物。
本发明所述积雪草又名雷公根,为伞形科植物,拉丁学名为:Centellaasiatica(L.)Urban,在我国已经有两千多年的应用历史,提取部位为全草,积雪草可以以水、酒精等为溶剂,按常规方法提取得到。积雪草主要含多种α-香树脂醇型的三萜成分,其中有积雪草甙、参枯尼甙、异参枯尼甙、羟基积雪草甙、玻热模甙、玻热米甙和玻热米酸等,以及马达积雪草酸,此外,尚含内消旋肌醇、积雪草糖、蜡、胡萝卜烃类、叶绿素,以及山柰酚、槲皮素和葡萄糖、鼠李糖的黄酮甙。本发明所制得的积雪草提取物的化妆品中文名称为积雪草(CENTELLA ASIATICA)提取物。
实施例1
一种复合植物精粹,由以下方法制备得到,具体步骤如下:
1)称取干燥后的小白菊200g,金黄洋甘菊1000g,母菊100g,混合,粉碎至20目得原料粉末;
2)加2倍原料粉末重量的水至原料粉末中,浸泡2小时,匀浆,得浆液;
3)浆液调节PH值至5然后加入浆液重量2wt%的纤维素酶,加乙醇至乙醇的终浓度为65%(体积分数),调节超声频率为40KHZ,在30℃条件下超声40min得提取液;
4)提取液过滤,在真空度为0.08Mpa,温度45℃下减压蒸馏浓缩除去乙醇,过滤,1000rpm离心30分钟,得复合植物精粹。
实施例2
一种复合植物精粹,由以下方法制备得到,具体步骤如下:
1)称取干燥后的小白菊800g,金黄洋甘菊100g,母菊1000g,混合,粉碎至80目得原料粉末;
2)加3倍原料粉末重量的水至原料粉末中,浸泡1小时,匀浆,得浆液;
3)浆液调节PH值至6,然后加入浆液重量1wt%的纤维素酶,加乙醇至乙醇的终浓度为75%(体积分数),调节超声频率为30KHZ,在55℃条件下超声20min得提取液;
4)提取液过滤,在真空度为0.1Mpa,温度40℃下减压蒸馏浓缩除去乙醇,过滤,5000rpm离心10分钟,得复合植物精粹。
实施例3
一种复合植物精粹,由以下方法制备得到,具体步骤如下:
1)称取干燥后的小白菊500g,金黄洋甘菊500g,母菊500g,混合,粉碎至50目得原料粉末;
2)加2倍原料粉末重量的水至原料粉末中,浸泡1.5小时,匀浆,得浆液;
3)浆液调节PH值至5.5,然后加入浆液重量1.5wt%的纤维素酶,加乙醇至乙醇的终浓度为70%(体积分数),调节超声频率为35KHZ,在45℃条件下超声30min得提取液;
4)提取液过滤,在真空度为0.09Mpa,温度43℃下减压蒸馏浓缩除去乙醇,过滤,3000rpm离心20分钟,得复合植物精粹。
实施例4
一种复合植物精粹,由以下方法制备得到,具体步骤如下:
1)称取干燥后的小白菊500g,金黄洋甘菊500g,母菊500g,花椒200g,积雪草800g,混合,粉碎至50目得原料粉末;
2)加2倍原料粉末重量的水至原料粉末中,浸泡1.5小时,匀浆,得浆液;
3)浆液调节PH值至5.5,然后加入浆液重量1.5wt%的纤维素酶,加乙醇至乙醇的终浓度为70%(体积分数),调节超声频率为35KHZ,在45℃条件下超声30min得提取液;
4)提取液过滤,在真空度为0.09Mpa,温度43℃下减压蒸馏浓缩除去乙醇,过滤,3000rpm离心20分钟,得复合植物精粹。
实施例5
一种复合植物精粹,由以下方法制备得到,具体步骤如下:
1)称取干燥后的小白菊500g,金黄洋甘菊500g,母菊500g,花椒800g,积雪草200g,混合,粉碎至50目得原料粉末;
2)加2倍原料粉末重量的水至原料粉末中,浸泡1.5小时,匀浆,得浆液;
3)浆液调节PH值至5.5,然后加入浆液重量1.5wt%的纤维素酶,加乙醇至乙醇的终浓度为70%(体积分数),调节超声频率为35KHZ,在45℃条件下超声30min得提取液;
4)提取液过滤,在真空度为0.09Mpa,温度43℃下减压蒸馏浓缩除去乙醇,过滤,3000rpm离心20分钟,得复合植物精粹。
实施例6
一种复合植物精粹,由以下方法制备得到,具体步骤如下:
1)称取干燥后的小白菊500g,金黄洋甘菊500g,母菊500g,花椒500g,积雪草500g,混合,粉碎至50目得原料粉末;
2)加2倍原料粉末重量的水至原料粉末中,浸泡1.5小时,匀浆,得浆液;
3)浆液调节PH值至5.5,然后加入浆液重量1.5wt%的纤维素酶,加乙醇至乙醇的终浓度为70%(体积分数),调节超声频率为35KHZ,在45℃条件下超声30min得提取液;
4)提取液过滤,在真空度为0.09Mpa,温度43℃下减压蒸馏浓缩除去乙醇,过滤,3000rpm离心20分钟,得复合植物精粹。
对比例1
一种小白菊提取物,由以下方法制备得到,具体步骤如下:
1)称取干燥后的小白菊1500g,粉碎至50目得原料粉末;
2)加2倍原料粉末重量的水至原料粉末中,浸泡1.5小时,匀浆,得浆液;
3)浆液调节PH值至5.5,然后加入浆液重量1.5wt%的纤维素酶,加乙醇至乙醇的终浓度为70%(体积分数),调节超声频率为35KHZ,在45℃条件下超声30min得提取液;
4)提取液过滤,在真空度为0.09Mpa,温度43℃下减压蒸馏浓缩除去乙醇,过滤,3000rpm离心20分钟,得小白菊提取物。
对比例2
一种金黄洋甘菊提取物,由以下方法制备得到,具体步骤如下:
1)称取干燥后的金黄洋甘菊1500g,粉碎至50目得原料粉末;
2)加2倍原料粉末重量的水至原料粉末中,浸泡1.5小时,匀浆,得浆液;
3)浆液调节PH值至5.5,然后加入浆液重量1.5wt%的纤维素酶,加乙醇至乙醇的终浓度为70%(体积分数),调节超声频率为35KHZ,在45℃条件下超声30min得提取液;
4)提取液过滤,在真空度为0.09Mpa,温度43℃下减压蒸馏浓缩除去乙醇,过滤,3000rpm离心20分钟,得金黄洋甘菊提取物。
对比例3
一种母菊提取物,由以下方法制备得到,具体步骤如下:
1)称取干燥后的母菊1500g,粉碎至50目得原料粉末;
2)加2倍原料粉末重量的水至原料粉末中,浸泡1.5小时,匀浆,得浆液;
3)浆液调节PH值至5.5,然后加入浆液重量1.5wt%的纤维素酶,加乙醇至乙醇的终浓度为70%(体积分数),调节超声频率为35KHZ,在45℃条件下超声30min得提取液;
4)提取液过滤,在真空度为0.09Mpa,温度43℃下减压蒸馏浓缩除去乙醇,过滤,3000rpm离心20分钟,得母菊提取物。
对比例4
一种复合植物精粹,由以下方法制备得到,具体步骤如下:
1)称取干燥后的小白菊500g,金黄洋甘菊500g,母菊500g,花椒1000g,混合,粉碎至50目得原料粉末;
2)加2倍原料粉末重量的水至原料粉末中,浸泡1.5小时,匀浆,得浆液;
3)浆液调节PH值至5.5,然后加入浆液重量1.5wt%的纤维素酶,加乙醇至乙醇的终浓度为70%(体积分数),调节超声频率为35KHZ,在45℃条件下超声30min得提取液;
4)提取液过滤,在真空度为0.09Mpa,温度43℃下减压蒸馏浓缩除去乙醇,过滤,3000rpm离心20分钟,得复合植物精粹。
对比例5
一种复合植物精粹,由以下方法制备得到,具体步骤如下:
1)称取干燥后的小白菊500g,金黄洋甘菊500g,母菊500g,积雪草1000g,混合,粉碎至50目得原料粉末;
2)加2倍原料粉末重量的水至原料粉末中,浸泡1.5小时,匀浆,得浆液;
3)浆液调节PH值至5.5,然后加入浆液重量1.5wt%的纤维素酶,加乙醇至乙醇的终浓度为70%(体积分数),调节超声频率为35KHZ,在45℃条件下超声30min得提取液;
4)提取液过滤,在真空度为0.09Mpa,温度43℃下减压蒸馏浓缩除去乙醇,过滤,3000rpm离心20分钟,得复合植物精粹。
实验1 抑制细胞DNA损伤和泄露
DNA存储着生物体赖以生存和繁衍的遗传信息,因此维护DNA分子的完整性对细胞及生物体至关紧要。紫外线中的UVB(280-320 nm)照射会导致皮肤出现红斑、色素沉着以及DNA损伤或改变,如果DNA的损伤或遗传信息的改变不能更正,可能影响细胞的功能或生存。所以在进化过程中生物细胞所获得的修复DNA损伤的能力就显得十分重要,也是生物能保持遗传稳定性之所在。抑制细胞DNA损伤和泄露可以提高皮肤免疫力和抵抗力,以应对恶劣环境中对辐射或其他刺激源的损害。
实验方法参考王海涛.化妆品用抗敏抗刺激活性物质的筛选、提取及作用机理研究.北京工商大学2010:66 第5.4.5小节的方法。
实验采用1.0%、5.0%两个梯度的实施例1-6和对比例1-3所得提取物作为测试浓度(实验时稀释20倍使用),分别测定其对SDS水溶液(其浓度为40μg/mL)诱导DNA损伤的保护作用,测试5次平行实验,取其平均值。
通过OxiSelectTM彗星分析试剂盒的观察,DNA受到的损坏愈大,DNA碎段就愈多,愈小的DNA碎段游离的速度就愈快,也游离的愈远,进而形成了彗星的尾部,而较大的一些碎段位置则靠近细胞核,因而形成彗星的头部。DNA碎段游动的程度不同使图像呈现出彗星状。彗星的长度与DNA的损害程度呈正相关性,彗星尾端愈长,细胞的损害程度愈大。
表1 抑制细胞DNA损伤彗星实验结果
由上表1可见,在添加实施例1-6所得复合植物精粹保护,然后用SDS刺激时,发生明显的DNA损伤减弱现象,其DNA损伤细胞拖尾率和彗尾长度均发生显著性的降低。对比例1-3制得提取物对抑制细胞DNA损伤效果较不理想。
以上实验结果表明,实施例1-6所得复合植物精粹对DNA的损伤和泄露有很好的抑制作用。
实验2 人群测试
制备如表2-3所示组成的修护精华露实验组1-6和对照组1-5,由以下质量百分含量的组分组成:
表2修护精华露实验组1-6组分表
表3 修护精华露对照组1-5组分表
实验方法:
1.实验对象:选取共360例症状表现为皮肤疼痛、干燥、脱皮、瘙痒的皮肤受损受试志愿者,男140例,女220例,年龄最大45岁,最小21岁,随机均分为12组,每组30人。2.样品:修护精华露实验组1-6和对照组1-6制得的样品。
3.使用方法:每日于肌肤受损部位涂受试样品,早晚各一次,每次使用量为0.1g/10cm2。使用4天、7天后统计使用效果。4.疗效判定标准(1)治愈:皮肤疼痛、干燥、脱皮、瘙痒等症状消失,皮肤变柔嫩、滋润、有光泽;(2)有效:皮肤疼痛、干燥、脱皮、瘙痒等症状消减;(3)无效:受损症状未见好转或加重。
将试验后的数据进行记录,治愈所用天数越少,相同时间内治愈人数越多则药效越好。试用结果见表4。
表4 受试者症状表现与疗效记录表
5.结果分析
(1)针对受损皮肤疼痛、干燥、脱皮、瘙痒等症状,使用实验组1-6修护精华露样品7天,有效率可达100%,其中使用实验组4-6的样品7天后,试用者皮肤疼痛、干燥、脱皮、瘙痒等症状完全消失,皮肤变柔嫩、滋润、有光泽。
(2)由实验组1-3与对照组1-3对比得知,小白菊、金黄洋甘菊、母菊复配制备得到的复合植物精粹,比单独采用小白菊、金黄洋甘菊、母菊中一个成分制备得到的提取物,对受损皮肤修护有更好的效果(使用实验组1-3的样品7天后,治愈23-27例,使用对照组1-3的样品7天后,治愈人数只有1-2人)。由实验组1-3与对照组4对比得知,用小白菊、金黄洋甘菊、母菊的混合物所制得提取液,其肌肤修护效果显著优于先将小白菊、金黄洋甘菊、母菊单独提取,后混合所制得提取液的效果。
(3)由实验组4-6与实验组1-3、对照组5-6对比得知,同时添加花椒、积雪草制备得到的复合植物精粹,比单独添加花椒或积雪草时制得的复合植物精粹,可以极大提升修护效果,可以让皮肤变得更加柔嫩、滋润、有光泽。
对使用实验组1-6制得的修护精华露样品的受试者回访发现,使用本发明制得样品后对皮肤无刺激,无毒副作用。
以上所述,仅是本发明的较佳实施例而已,并非对本发明做任何形式上的限制,故凡未脱离本发明技术方案的内容,依据本发明的技术实质对以上实施例所做的任何简单修改、等同变化与修饰,均仍属于本发明技术方案的范围内。
Claims (8)
1.一种修护精华露,其特征在于,主要由以下质量百分含量的各组分组成:丁二醇1-5%,玉米谷蛋白氨基酸类0.5-5%,水解胶原0.5-2.5%,银耳(TREMELLA FUCIFORMIS)提取物0.01-0.2%,泛醇0.1-1%,硅烷三醇1-5%,寡肽-1的1‰水溶液0.1-2%,寡肽-2 的1‰水溶液0.1-2%,寡肽-5 的1‰水溶液0.1-2%,神经酰胺3 0.01-0.1%,乙酰基六肽-8的1‰水溶液0.1-1%,叶酸0.01-0.1%,芽孢杆菌/大豆发酵产物提取物0.1-1%,透明质酸钠0.01-0.1%,磷脂0.01-0.1%,茶(CAMELLIA SINENSIS)提取物0.1-2%,木兰醇0.1-2%,薄荷(MENTHAARVENSIS)叶提取物0.1-2%,马齿苋(PORTULACA OLERACEA)提取物0.1-2%,库拉索芦荟(ALOE BARBADENSIS)提取物0.1-2%,苦参(SOPHORA FLAVESCENS)提取物0.1-1%,黄芩(SCUTELLARIA BAICALENSIS)根提取物0.1-1%,茶(CAMELLIA SINENSIS)叶提取物0.1-1%,光果甘草(GLYCYRRHIZA GLABRA)提取物0.1-1%,迷迭香(ROSMARINUS OFFICINALIS)叶提取物0.1-1%,虎杖(POLYGONUM CUSPIDATUM)根提取物0.1-1%,柚(CITRUS GRANDIS)果提取物0.1-1%,己二醇0.1-1%,复合植物精粹0.1-10%,水45-90%;其中所述的复合植物精粹主要由小白菊、金黄洋甘菊、母菊提取得到。
2.根据权利要求1所述的修护精华露,其特征在于,所述复合植物精粹主要由以下重量份原料提取得到:小白菊2-8份,金黄洋甘菊1-10份,母菊1-10份。
3.根据权利要求1或2所述的修护精华露,其特征在于,所述复合植物精粹由以下重量份原料提取得到:小白菊2-8份,金黄洋甘菊1-10份,母菊1-10份,花椒2-8份,积雪草2-8份。
4.根据权利要求1-3任一项所述的修护精华露,其特征在于,所述的复合植物精粹由以下方法制备得到,具体步骤如下:
1)称取干燥后的原料,混合,粉碎得原料粉末;
2)将原料粉末加水浸泡,匀浆,得浆液;
3)浆液调节PH值至5-6,然后加入纤维素酶,加乙醇,超声辅助提取得提取液;
4)提取液过滤,减压浓缩除去乙醇,过滤,离心,得复合植物精粹。
5.根据权利要求4所述的修护精华露,其特征在于,步骤1)中所述原料粉碎至20-80目。
6.根据权利要求4所述的修护精华露,其特征在于,步骤2)中所述的浸泡条件为加原料粉末重量2-3倍的水,浸泡1-2小时。
7.根据权利要求4所述的修护精华露,其特征在于,步骤3)中所述加入纤维素酶的量为浆液重量的1-2wt%;所述乙醇的加入终浓度为65-75%(体积分数);所述超声条件为:频率30-40KHZ,温度为30-55℃,作用时间为20-40min。
8.根据权利要求4所述的修护精华露,其特征在于,步骤4)中所述减压浓缩条件为在真空度为0.08-0.1Mpa,温度40℃-45℃下减压蒸馏;所述离心为1000-5000rpm离心10-30分钟。
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