CN110100945B - Hemp blood fat reducing peptide composition and application thereof - Google Patents
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- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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Abstract
The invention belongs to the technical field of bioactive peptides, and particularly relates to a hemp blood fat reducing peptide composition and application thereof. In order to solve the problem of insufficient utilization of hemp seed meal resources, the invention provides a hemp blood fat reducing peptide composition which comprises pentapeptide with an amino acid sequence of Val-Ser-Glu-Pro-Arg and hexapeptide with an amino acid sequence of Ile-Asp-Lys-Phe-Ser-Arg in a mass ratio of 4: 5. The small molecular peptide composition obtained by carrying out enzymolysis on the hemp seed meal and filtering by using a 1KD ultrafiltration membrane has higher content of glutamic acid and arginine, and the special amino acid sequence ensures that the hemp blood fat reducing peptide composition has obvious blood fat regulating effect in human body absorption and metabolism.
Description
Technical Field
The invention belongs to the technical field of bioactive peptides, and particularly relates to a hemp blood fat reducing peptide composition and application thereof.
Background
Dyslipidemia is one of independent and important risk factors of cardiovascular and cerebrovascular diseases such as coronary heart disease, myocardial infarction, ischemic stroke and the like, and seriously harms human health. At present, the clinical application of the chemical drugs such as acipimox, simvastatin, cholestyramine and the like for treating hyperlipidemia has good curative effect, but a plurality of adverse reactions such as liver and kidney injury, rhabdomyolysis and the like can be generated after long-term administration. Therefore, screening safe and side-effect-free blood fat-reducing active substances from natural animal and plant resources becomes a new trend for researchers at home and abroad.
Bioactive peptides refer to peptide compounds that are beneficial to or have a physiological effect on the vital activities of the living organism. The structure of bioactive peptides can range from simple dipeptides to larger polypeptides. As basic nutrient substance, active peptide has high bioavailability, is easier to be absorbed than protein, and has multiple biological functions such as immunoregulation, antithrombotic, blood pressure lowering, cholesterol lowering, anticancer, antioxidant, etc.
Hemp (Cannabis sativa) is an annual herb plant of the genus Cannabis of the family Cannabaceae, also known as Cannabis sativa, and is one of the oldest cultivated crops. The hemp seeds are used as food and oil, and contain rich nutrient components, including about 25-35% of fatty acid, 20-25% of protein, 20-30% of carbohydrate, 10-15% of fiber and various mineral substances. Because it contains abundant essential amino acids and essential fatty acids and has reasonable composition ratio, it is considered as the most complete nutrient source nowadays and is an ideal source of bioactive polypeptide.
However, in most hemp production areas, hemp seeds are only used for extracting hemp seed oil, and hemp seed meal containing rich protein is discarded or used as feed, which results in waste of hemp seed resources.
Disclosure of Invention
In order to solve the problem of insufficient utilization of hemp seed meal resources, the invention provides a hemp blood fat reducing peptide composition and application thereof.
The technical scheme of the invention is as follows:
a hemp blood fat reducing peptide composition mainly comprises pentapeptide with an amino acid sequence of Val-Ser-Glu-Pro-Arg and hexapeptide with an amino acid sequence of Ile-Asp-Lys-Phe-Ser-Arg.
Further, the mass ratio of the pentapeptide to the hexapeptide is 4: 5.
Further, the molecular weight of the pentapeptide is 680 Da.
Furthermore, the content of glutamic acid in the pentapeptide is 140.97mg/g, and the content of arginine in the pentapeptide is 89.0 mg/g.
Further, the hexapeptide has a molecular weight of 876 Da.
Further, the preparation method of the hemp blood fat reducing peptide composition comprises the following steps: mixing the hemp seed meal and distilled water according to a certain material-liquid ratio, adding papain and neutral protease into the mixed solution, carrying out enzymolysis at a certain temperature to obtain an enzymolysis solution, heating to inactivate the enzyme, centrifuging the enzymolysis solution, taking supernatant, filtering by a 1KD ultrafiltration membrane to obtain a hemp blood fat reducing peptide composition with the molecular weight less than 1KD, and carrying out low-temperature freeze-drying to obtain the hemp blood fat reducing peptide freeze-dried powder.
Further, the feed-liquid ratio is 1g to 10 g.
Further, the enzyme activity of the papain is 80 ten thousand u/g, and the addition amount of the papain is 0.2-0.6% of the mass of the mixed solution; the enzyme activity of the neutral protease is 13 ten thousand u/g, and the addition amount of the neutral protease is 2.0-3.0% of the mass of the mixed solution.
Further, the enzymolysis temperature is 60-70 ℃, and the enzymolysis time is 1-3 hours; the enzyme deactivation temperature is 90-100 ℃, and the enzyme deactivation time is 10-20 min.
The invention relates to an application of a hemp blood fat reducing peptide composition in blood fat reducing medicines, health-care foods and food additives.
The invention has the beneficial effects that:
the content of essential amino acid contained in the pentapeptide in the hemp blood fat reducing peptide composition provided by the invention accounts for 25.19% of the total amino acid, and the content of glutamic acid and arginine in the pentapeptide is higher and is 140.97mg/g and 89.0mg/g respectively. The special amino acid sequence enables the hemp blood fat reducing peptide composition to show a remarkable blood fat regulating effect in human absorption and metabolism, can block the absorption of cholesterol in intestinal tracts and promote the cholesterol to be discharged out of the body, thereby reducing the concentration of blood cholesterol. The adsorption rates of the hemp blood fat reducing peptide composition on sodium cholate, sodium taurocholate and sodium glycocholate are 85.02%, 25.12% and 58.10% respectively.
The hemp seed meal is a raw material of medicinal and edible food, fully exerts the nutrition and medical health care efficacy of the hemp seed polypeptide, expands the application of the hemp seed polypeptide in the fields of functional food, medicine and the like, improves the additional value of deep processing of the hemp seed, has simple preparation process and is suitable for large-scale production.
Detailed Description
The technical solutions of the present invention are further described below with reference to the following examples, but the present invention is not limited thereto, and any modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Example 1
A hemp blood fat reducing peptide composition mainly comprises pentapeptide with an amino acid sequence of Val-Ser-Glu-Pro-Arg and hexapeptide with an amino acid sequence of Ile-Asp-Lys-Phe-Ser-Arg.
Example 2
A hemp blood fat reducing peptide composition mainly comprises a pentapeptide with an amino acid sequence of Val-Ser-Glu-Pro-Arg and a hexapeptide with an amino acid sequence of Ile-Asp-Lys-Phe-Ser-Arg, wherein the mass ratio of the pentapeptide to the hexapeptide is 4: 5.
The molecular weight of the pentapeptide is 680Da, the content of glutamic acid is 140.97mg/g, the content of arginine is 89.0mg/g, and the content of essential amino acid accounts for 25.19% of the total amino acid.
The hexapeptide has a molecular weight of 876 Da.
Example 3
The embodiment provides a preparation method of a hemp blood fat reducing peptide composition, which comprises the following steps:
mixing the hemp seed meal and distilled water according to a certain material-liquid ratio, adding papain and neutral protease into the mixed solution, carrying out enzymolysis at a certain temperature to obtain an enzymolysis solution, heating to inactivate the enzyme, centrifuging the enzymolysis solution, taking supernatant, filtering by a 1KD ultrafiltration membrane to obtain a hemp blood fat reducing peptide composition with the molecular weight less than 1KD, and carrying out low-temperature freeze-drying to obtain the hemp blood fat reducing peptide freeze-dried powder.
Example 4
The embodiment provides a preparation method of a hemp blood fat reducing peptide composition, which comprises the following steps:
mixing the hemp seed meal and distilled water according to a material-liquid ratio of 1g to 10g, adding papain and neutral protease into the mixed solution, wherein the enzyme activity of the papain is 80 ten thousand u/g, and the addition amount is 0.2-0.6% of the mass of the mixed solution; the enzyme activity of the neutral protease is 13 ten thousand u/g, and the adding amount is 2.0-3.0% of the mass of the mixed solution.
Carrying out enzymolysis for 1-3 h at the temperature of 60-70 ℃ to obtain an enzymolysis liquid, heating the enzymolysis liquid to 90-100 ℃, inactivating enzyme for 10-20 min, centrifuging the enzymolysis liquid, taking supernatant, filtering by a 1KD ultrafiltration membrane to obtain a China hemp blood fat reducing peptide composition with the molecular weight less than 1KD, and freeze-drying at low temperature to obtain the China hemp blood fat reducing peptide freeze-dried powder.
Example 5
The embodiment provides a preparation method of a hemp blood fat reducing peptide composition, which comprises the following steps:
mixing the hemp seed meal and distilled water according to a material-liquid ratio of 1g to 10g, adding papain and neutral protease into the mixed solution, wherein the enzyme activity of the papain is 80 ten thousand u/g, and the addition amount is 0.2-0.6% of the mass of the mixed solution; the enzyme activity of the neutral protease is 13 ten thousand u/g, and the adding amount is 2.0-3.0% of the mass of the mixed solution.
Carrying out enzymolysis for 2.5h at 65 ℃ to obtain an enzymolysis liquid, heating the enzymolysis liquid to 95 ℃ to inactivate enzyme for 15min to inactivate the enzyme, centrifuging the enzymolysis liquid, taking supernatant, filtering by a 1KD ultrafiltration membrane to obtain the China hemp blood fat reducing peptide composition with the molecular weight less than 1KD, and freeze-drying at low temperature to obtain the China hemp blood fat reducing peptide freeze-dried powder.
Example 6
The hemp seed meal polypeptides with different molecular weights are prepared by the embodiment, and the preparation method comprises the following steps:
mixing the hemp seed meal and distilled water according to a material-liquid ratio of 1g to 10g, adding papain and neutral protease into the mixed solution, wherein the enzyme activity of the papain is 80 ten thousand u/g, and the addition amount is 0.2-0.6% of the mass of the mixed solution; the enzyme activity of the neutral protease is 13 ten thousand u/g, and the adding amount is 2.0-3.0% of the mass of the mixed solution.
Carrying out enzymolysis for 2.5h at the temperature of 65 ℃ to obtain an enzymolysis liquid, heating the enzymolysis liquid to 95 ℃ to inactivate enzyme for 15min to inactivate the enzyme, centrifuging the enzymolysis liquid, taking supernatant, separating the enzymolysis hemp seed meal polypeptide mixed liquid by adopting an ultrafiltration membrane filtration system, and separating the mixed polypeptide by adopting ultrafiltration membranes with the cut-off molecular weights of 1KD, 3KD and 5KD to obtain 4 polypeptide components with different molecular weights, wherein each component and the molecular weight range are shown in a table 1:
TABLE 1
| Categories | Component a | Component b | Component c | Component d |
| Molecular weight range | >5KD | 3KD-5KD | 1KD-3KD | <1KD |
The 4 separated polypeptide components with different molecular weights are evaluated for the capacity of combining cholate, and the evaluation method is as follows:
(1) drawing a standard curve of cholate
Preparing cholate standard solution: cholate 0, 20, 40, 60, 80, 100, 120, 140, 160, 180, and 200mg each were accurately weighed into 100mL volumetric flasks, dissolved in distilled water and added to the mark. 1mL of cholate standard solution with different mass concentrations is taken to be put into a test tube with a plug, 6mL of 45% sulfuric acid is added, and the mixture is uniformly mixed; adding 1mL of 0.3% furfural, and mixing uniformly; reacting in a constant temperature water bath at 65 ℃ for 30min, cooling to room temperature, measuring absorbance at the wavelength of 620nm, and drawing a standard curve.
(2) Determination of adsorption effect of hemp seed polypeptide on cholate
Respectively sucking 5-10 mg/ml of four polypeptide components of a component a, b, c and d, respectively adding 0.5-2 ml of artificial gastric juice, and carrying out thermostatic water bath for 1-3 h at 37 ℃; adjusting the pH value to 6-7, adding 1-3 ml of artificial intestinal juice, and oscillating at a constant temperature of 37 ℃ for 1-3 h; respectively adding 2ml of sodium taurocholate, sodium cholate and sodium glycocholate, shaking at the constant temperature of 37 ℃ for 1-3 h, centrifuging, taking 1ml of supernate, adding 6ml of 45% sulfuric acid and 1ml of 0.3% furfural, and carrying out constant-temperature water bath for 30-60 min at the temperature of 65 ℃; detecting the absorbance at 620nm, calculating the adsorption rate of the four polypeptide components to cholate according to the absorbance value, and the experimental result is shown in Table 2,
TABLE 2
As can be seen from Table 2, the hemp seed polypeptide component has an adsorption effect on 3 representative cholates, wherein the adsorption capacity of the component d, namely the hemp blood fat reducing peptide composition, is obviously greater than that of the other three components, the adsorption rate on sodium cholate is 85.02%, and the adsorption rate on sodium taurocholate which is difficult to be adsorbed is also higher than 20%, and reaches 25.12%. Therefore, functional factors with stronger hypolipidemic effect in the component d can be preliminarily determined. Experiments show that sodium cholate, sodium glycocholate and sodium taurocholate are used as adsorption objects, so that the hypolipidemic mechanism that the hemp seed polypeptide inhibits the absorption of cholate in intestinal tracts and discharges the cholate out of the bodies is disclosed, the metabolism of cholesterol in the bodies is converted into bile acid is promoted, and the hypolipidemic effect of the hemp seed polypeptide is proved.
Example 7
Essential amino acid compositions and nonessential amino acid compositions of four polypeptide fractions a, b, c and d obtained in example 6 were measured using an automatic amino acid analyzer, and the results are shown in tables 3 and 4,
TABLE 3
TABLE 4
As shown in tables 3 and 4, the content of glutamic acid is the highest and the content of arginine is the second highest in the hemp seed polypeptide components with different molecular weights. The content of essential amino acids in each of the polypeptide components a, b, c and d is 23.83%, 24.46%, 22.83% and 25.19%, wherein the component d, namely the essential amino acid content in the hemp blood fat reducing peptide composition is the highest, and the special amino acid sequence enables the hemp blood fat reducing peptide composition to show a remarkable blood fat regulating effect in human body absorption and metabolism.
Example 8
This example analyzes the amino acid sequence of fraction d obtained in example 6, i.e., the lipid-lowering peptide composition of hemp, by MALDI-TOF/TOF mass spectrometry. Applying 1 μ L of sample to the target, drying naturally, applying 1 μ L of CHCA matrix solution to the corresponding target, drying naturally, and applying the same method to calibrate the standard (4700 proteins Analyzer Calibration mix 1) on the target adjacent to the target. Performing external standard first-stage Calibration by adopting a Calibration standard Calibration Mixture 1, wherein the mass error is less than 0.5 Da; collecting the conditions of the atlas: primary Mass Spectrum (MS) range: 800-4000m/z, and 500 Laser Shots are accumulated in each spectrogram; selecting a target polypeptide of a test sample corresponding to a parent ion to perform secondary mass spectrometry (MS/MS), accumulating 2500 Laser Shots in each spectrogram, and respectively adjusting the Laser intensity and the gas pressure of a collision Cell (CID) to obtain the optimal spectrogram under the premise of fixing the main parameters.
The detection and analysis result shows that the hemp blood fat reducing peptide composition mainly comprises pentapeptide with the amino acid sequence of Val-Ser-Glu-Pro-Arg and hexapeptide with the amino acid sequence of Ile-Asp-Lys-Phe-Ser-Arg.
SEQUENCE LISTING
<110> Daqing division of department academy of sciences of Heilongjiang province
<120> China hemp blood fat reducing peptide composition and application thereof
<130> 1
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 5
<212> PRT
<213> hemp
<400> 1
Val Ser Glu Pro Arg
1 5
<210> 2
<211> 6
<212> PRT
<213> hemp
<400> 2
Ile Asp Lys Phe Ser Arg
1 5
Claims (4)
1. The hemp blood fat reducing peptide composition is characterized by mainly comprising pentapeptide with an amino acid sequence of Val-Ser-Glu-Pro-Arg and hexapeptide with an amino acid sequence of Ile-Asp-Lys-Phe-Ser-Arg;
the preparation method of the composition comprises the following steps: mixing the hemp seed meal and distilled water according to a certain material-liquid ratio, adding papain and neutral protease into the mixed solution, carrying out enzymolysis at a certain temperature to obtain an enzymolysis solution, heating to inactivate the enzyme, centrifuging the enzymolysis solution, taking supernatant, filtering by a 1KD ultrafiltration membrane to obtain a hemp blood fat reducing peptide composition with the molecular weight less than 1KD, and carrying out low-temperature freeze-drying to obtain a hemp blood fat reducing peptide freeze-dried powder;
the enzymolysis temperature is 65 ℃, and the enzymolysis time is 2.5 h; the enzyme deactivation temperature is 90-100 ℃, and the enzyme deactivation time is 10-20 min;
the mass ratio of the pentapeptide to the hexapeptide is 4: 5; the feed-liquid ratio is 1g to 10 g; the enzyme activity of the papain is 80 ten thousand u/g, and the addition amount of the papain is 0.2-0.6% of the mass of the mixed solution; the enzyme activity of the neutral protease is 13 ten thousand u/g, and the addition amount of the neutral protease is 2.0-3.0% of the mass of the mixed solution.
2. The lipid-lowering peptide composition of claim 1, wherein the molecular weight of said pentapeptide is 680 Da.
3. The lipid-lowering peptide composition of claim 1, wherein the hexapeptide has a molecular weight of 876 Da.
4. The use of the lipid-lowering peptide composition of hemp as claimed in any one of claims 1 to 3 for the preparation of lipid-lowering drugs, health foods and food additives.
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