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CN110089677A - A kind of sour powder, preparation method thereof improving steamed bread quality - Google Patents

A kind of sour powder, preparation method thereof improving steamed bread quality Download PDF

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CN110089677A
CN110089677A CN201910320979.3A CN201910320979A CN110089677A CN 110089677 A CN110089677 A CN 110089677A CN 201910320979 A CN201910320979 A CN 201910320979A CN 110089677 A CN110089677 A CN 110089677A
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san francisco
lactobacillus
lactobacillus san
cornmeal
wheat flour
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张国华
唐宏宇
赵明利
赵敬亭
张纬珍
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Shanxi University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/104Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/183Sanfranciscenis

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  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

本发明属于食品技术领域,具体一种改善馒头品质的酸粉制备方法。由以下重量配比的原料制备而成:小麦面粉150~200 g;55~75 g玉米面;旧金山乳杆菌Ls‑1001发酵液150~200 mL(其中菌落数为2.04×109CFU/mL)。玉米面的添加相比仅加入小麦面粉发酵出来的馒头香气更浓,且玉米面中富含人体所需的膳食纤维,有利于人体肠道蠕动。与此同时,从老面分离出来的的旧金山乳杆菌在发酵过程中产气,使馒头更加松软,质地更加细腻,改善面制品色泽,具有更好的弹性口感和独特风味。The invention belongs to the technical field of food, in particular to a method for preparing acid powder for improving the quality of steamed bread. It is prepared from the raw materials with the following weight ratio: 150-200 g of wheat flour; 55-75 g of cornmeal; 150-200 mL of Lactobacillus San Francisco Ls-1001 fermentation broth (the number of colonies is 2.04×109CFU/mL). The addition of cornmeal has a stronger aroma than steamed buns fermented with only wheat flour, and cornmeal is rich in dietary fiber needed by the human body, which is conducive to human intestinal peristalsis. At the same time, the Lactobacillus San Francisco isolated from the old noodles produces gas during the fermentation process, which makes the steamed buns softer and more delicate, improves the color of the noodle products, and has a better elastic taste and unique flavor.

Description

一种改善馒头品质的酸粉制备方法A preparation method of sour powder for improving the quality of steamed bread

技术领域technical field

本发明属于食品技术领域,具体一种改善馒头品质的酸粉制备方法。The invention belongs to the technical field of food, in particular to a method for preparing acid powder for improving the quality of steamed bread.

背景技术Background technique

老面发酵是一种原始的发酵方法,迄今为止已有几千年历史。它靠空气中野生酵母和各种杂菌进行发酵生产。老面是由酵母菌、乳酸菌及霉菌等组成的混菌发酵体系。不同温度、湿度和环境均能导致老面中的微生物菌群组成的不同。据报道,从老面中分离的乳酸菌超过60种,其中以乳杆菌属(Lactobacillus)的分布最为广泛,特别是旧金山乳杆菌是酸面团中的优势菌种。在面团发酵过程中,旧金山乳杆菌可利用麦芽糖,有助于糖化、蛋白分解、酯化和产气等过程,产生了丰富的代谢物质,因而发酵的面食感官品质更佳。此外,旧金山乳杆菌在发酵过程中产生的胞外多糖可以延缓面制品的老化,产生的抑菌物质能够有效抑制真菌与细菌的生长,从而延长面制品保藏时间。Old dough fermentation is a primitive fermentation method with a history of thousands of years. It is produced by fermentation of wild yeast and various bacteria in the air. Old noodle is a mixed fermentation system composed of yeast, lactic acid bacteria and mold. Different temperature, humidity and environment can lead to differences in the composition of microbial flora in aged noodles. According to reports, there are more than 60 species of lactic acid bacteria isolated from old noodles, among which Lactobacillus is the most widely distributed, especially Lactobacillus San Francisco is the dominant species in sourdough. During the dough fermentation process, Lactobacillus San Francisco can use maltose, which contributes to the processes of saccharification, proteolysis, esterification and gas production, and produces rich metabolites, so the sensory quality of fermented pasta is better. In addition, the exopolysaccharide produced by Lactobacillus San Francisco during the fermentation process can delay the aging of flour products, and the antibacterial substances produced can effectively inhibit the growth of fungi and bacteria, thereby prolonging the preservation time of flour products.

目前,市场上所用的高活性干酵母发酵能在短时间内发酵制成馒头,发酵温度一般为30 ℃~35 ℃,发酵时间为30~40 min,且发酵时不会使面团过酸或过碱。但与老面的混菌体系发酵相比,它发酵的馒头风味平淡、香气不佳,总体的感官品质低,复蒸性能较差。而使用传统老面发酵,除发酵温度较低(一般在30 ℃左右),由于老面中含有复杂微生物,可产生较多的风味物质(如醇类、醛类、酯类与挥发性酸等),使得老面发酵面制品的口味较浓厚,口感独特。At present, the highly active dry yeast used in the market can be fermented into steamed buns in a short period of time. The fermentation temperature is generally 30 ℃ ~ 35 ℃, and the fermentation time is 30 ~ 40 minutes, and the dough will not be too sour or overcooked during fermentation. alkali. However, compared with the fermentation of old noodles with mixed bacteria system, the steamed buns fermented by it have flat flavor, poor aroma, low overall sensory quality and poor re-steaming performance. In addition to the low fermentation temperature (generally around 30°C) of traditional old noodle fermentation, the old noodle contains complex microorganisms that can produce more flavor substances (such as alcohols, aldehydes, esters and volatile acids, etc.) ), which makes the fermented noodle products of old noodles have a stronger taste and a unique taste.

专利申请号为201810477518 .2公开了一种旧金山乳杆菌馒头及其制备方法,使用了小麦面粉; 安琪高活性干酵母; 旧金山乳杆菌Ls-1001发酵液。使用旧金山乳杆菌Ls-1001和少量小麦面粉搅拌成面糊,发酵成种面糊;再将种面糊与安琪高活性干酵母混合后加入小麦面粉中搅拌成团,发酵,醒发,蒸制 成旧金山乳杆菌馒头。Patent application number 201810477518.2 discloses a Lactobacillus San Francisco steamed bread and its preparation method, using wheat flour; Angel high-activity dry yeast; Lactobacillus San Francisco Ls-1001 fermentation broth. Stir the batter with Lactobacillus San Francisco Ls-1001 and a small amount of wheat flour, and ferment it into a seed batter; then mix the seed batter with Angel’s high-activity dry yeast, add it to the wheat flour, stir into a dough, ferment, proof, and steam to make San Francisco Lactobacillus bread.

因此,本发明利用分离自老面的优势菌旧金山乳杆菌对面团进行发酵,并辅配玉米面制成一款面食发酵剂,称之为“酸粉”。用酸粉与高活性干酵母混合发酵的面制品,结合了乳酸菌发酵和高活性干酵母发酵的优势,在具有短时间发酵优点的同时,又具有很高的营养价值和感官品质,且色泽均匀,复蒸性能良好,有类似于老面发酵的浓厚风味。此外酸粉还易于保存和运输,能有效促进馒头、面包等发酵面制品的工业化生产。Therefore, the present invention utilizes Lactobacillus San Francisco, a dominant bacterium isolated from old noodles, to ferment the dough, and supplemented with cornmeal to make a pasta starter, which is called "sour powder". The noodle product fermented with sour powder and high active dry yeast combines the advantages of lactic acid bacteria fermentation and high active dry yeast fermentation. It has the advantages of short fermentation time, high nutritional value and sensory quality, and uniform color , re-steaming performance is good, and it has a strong flavor similar to old noodle fermentation. In addition, the sour powder is also easy to store and transport, and can effectively promote the industrialized production of steamed bread, bread and other fermented flour products.

发明内容Contents of the invention

本发明为了改善当前市面上高活性干酵母在发酵面食方面微生物菌种单一、营养结构简单、风味单一等方面的不足,让“记忆中妈妈的味道”深入人心,本发明提供一种由分离自老面中的旧金山乳杆菌发酵制成的酸粉,可将其与高活性干酵母混合发酵用于日常发酵面制品中,从而优化营养结构,使传统发酵面制品具有更广泛的微生物多样性,并促进传统发酵面制品的工业化发展。In order to improve the deficiencies of high active dry yeast currently on the market in terms of single microbial strains, simple nutritional structure, and single flavor in fermenting pasta, and to make "the taste of mother in memory" deeply rooted in the hearts of the people, the present invention provides a The sour powder fermented by Lactobacillus San Francisco in old noodles can be mixed with highly active dry yeast and used in daily fermented noodle products, so as to optimize the nutritional structure and make traditional fermented noodle products have a wider microbial diversity. And promote the industrialization of traditional fermented flour products.

本发明采用的菌株为旧金山乳杆菌 Ls-1001,保藏单位:中国微生物菌种保藏管理委员会普通微生物中心,保藏地址:北京市朝阳区北辰西路1号院3号中国科学院微生物研究所,保藏日期:2017年6月12日,保藏编号:CGMCC No.14232,分类命名:旧金山乳杆菌Lactobacillus sanfrancisco。The bacterial strain used in the present invention is Lactobacillus San Francisco Ls-1001, preserved by: China Microbial Strains Preservation Management Committee General Microorganism Center, preserved at: No. 3, Institute of Microbiology, Chinese Academy of Sciences, No. 1, Beichen West Road, Chaoyang District, Beijing, preserved date : June 12, 2017, deposit number: CGMCC No.14232, classification name: Lactobacillus sanfrancisco.

本发明提供一种改善馒头品质的酸粉制备方法,由以下重量配比的原料制备而成:The invention provides a method for preparing acid powder for improving the quality of steamed bread, which is prepared from the following raw materials in weight ratio:

小麦面粉150~200 g;55~75 g玉米面;旧金山乳杆菌 Ls-1001发酵液150~200 mL(其中菌落数为 2.04×109 CFU/mL)。Wheat flour 150-200 g; cornmeal 55-75 g; Lactobacillus San Francisco Ls-1001 fermentation broth 150-200 mL (the number of colonies is 2.04×109 CFU/mL).

一种改善馒头品质的酸粉制备方法,它的制作包括下述步骤:A method for preparing sour powder for improving the quality of steamed bread, which comprises the following steps:

(1)将150~200 mL旧金山乳杆菌Ls-1001发酵液于4000 r/min的条件下离心4 min去上清液,得到旧金山乳杆菌 Ls-1001菌泥,将菌泥溶于80~120 mL纯净水并加入150~200 g小麦面粉搅拌成团,放入恒温箱发酵8~12 h;(1) Centrifuge 150-200 mL of Lactobacillus San Francisco Ls-1001 fermentation broth at 4000 r/min for 4 minutes to remove the supernatant to obtain Lactobacillus San Francisco Ls-1001 sludge, which is dissolved in 80-120 Add 150-200 g of wheat flour to 1 mL of pure water and stir to form a dough, put it into a constant temperature box to ferment for 8-12 hours;

(2)待其发酵好后向其中加入55~75 g玉米面再次揉匀并将其捏碎,放置到烘干箱中烘干,温度为30~35 ℃,时间5~10 h;(2) After it has been fermented, add 55-75 g of cornmeal to it, knead it evenly again, crush it, and place it in a drying oven for drying at a temperature of 30-35 °C for 5-10 hours;

(3)将烘干好的碎屑放入粉碎机粉碎成粉。(3) Put the dried crumbs into a grinder and grind them into powder.

以上所述的旧金山乳杆菌菌泥的制作方法是:将旧金山乳杆菌Ls-1001按体积分数为1%的接种量于 mMRS 培养基中并将其放入厌氧培养箱培养,培养时间24 h,培养温度35 ℃,厌氧条件为 N2、CO2、O2,其体积百分比为79:20:1。之后将培养好的旧金山乳杆菌Ls-1001 发酵液在转速为4000 r/min的条件下离心4~5 min,倒去上清液,收集沉淀得到旧金山乳杆菌菌泥。The preparation method of the above-mentioned Lactobacillus San Francisco sludge is as follows: the inoculation amount of Lactobacillus San Francisco Ls-1001 according to the volume fraction is 1% in the mMRS medium and put it into an anaerobic incubator for cultivation, and the cultivation time is 24 h , the culture temperature is 35 ℃, the anaerobic condition is N2, CO2, O2, and its volume percentage is 79:20:1. After that, the cultured Lactobacillus San Francisco Ls-1001 fermentation broth was centrifuged at 4000 r/min for 4-5 minutes, the supernatant was poured off, and the precipitate was collected to obtain Lactobacillus San Francisco sludge.

本发明的有益效果:Beneficial effects of the present invention:

玉米面的添加相比仅加入小麦面粉发酵出来的馒头香气更浓,且玉米面中富含人体所需的膳食纤维,有利于人体肠道蠕动。与此同时,从老面分离出来的的旧金山乳杆菌在发酵过程中产气,使馒头更加松软,质地更加细腻,改善面制品色泽,具有更好的弹性口感和独特风味。The addition of cornmeal has a stronger aroma than steamed buns fermented with only wheat flour, and cornmeal is rich in dietary fiber needed by the human body, which is conducive to human intestinal peristalsis. At the same time, the Lactobacillus San Francisco isolated from the old noodles produces gas during the fermentation process, which makes the steamed buns softer and more delicate, improves the color of the noodle products, and has a better elastic taste and unique flavor.

和背景技术中所述的201810477518 .2技术方案,本申请技术方案特点是先活菌一次发酵再干燥成酸粉利用酸粉来蒸馒头,创新点:1.一次发酵时间缩短,2.酸粉相比面糊更方便快捷3.本专利是干燥后的而不是活菌 4.复配了玉米面5.运用了烘干技术粉碎技术6.本技术方案的产品为发酵剂,可以用这款发酵剂来发酵多种面制品。Compared with the 201810477518.2 technical solution described in the background technology, the technical solution of this application is characterized in that live bacteria are fermented once and then dried into sour powder, and steamed buns are steamed with sour powder. More convenient and faster than batter 3. This patent is dried instead of live bacteria 4. Cornmeal is compounded 5. Drying technology and crushing technology are used 6. The product of this technical solution is a starter, which can be used To ferment a variety of flour products.

具体实施方式Detailed ways

测定感官评分规则:取冷却时间20 min内的馒头进行测定感官评分,再将其25±2℃放置,将馒头切成数块,根据下表 1 感官评分表,由十人品评小组进行品评,满分100分,试验三次重复,取均值。Determination of sensory scoring rules: Take the steamed buns within 20 minutes of cooling time for sensory scoring, then place them at 25±2°C, cut the steamed buns into several pieces, and evaluate them by a panel of ten people according to the sensory scoring table in Table 1 below. The full score is 100 points, the test is repeated three times, and the average value is taken.

表1 感官评分表Table 1 Sensory scoring table

实施例1:酸粉的制备(条件最佳)Embodiment 1: the preparation of acid powder (conditions are the best)

配料:小麦面粉200 g;旧金山乳杆菌Ls-1001发酵液200 mL(菌落数为2.04×109 CFU/mL);玉米面75 g;纯净水50 mL。Ingredients: 200 g of wheat flour; 200 mL of fermentation broth of Lactobacillus San Francisco Ls-1001 (the colony number is 2.04×109 CFU/mL); 75 g of cornmeal; 50 mL of purified water.

(1)将200 mL旧金山乳杆菌Ls-1001发酵液于于4000 r/min的条件下离心4 min去上清液,将得到的菌泥溶于50 mL纯净水后,加入200 g小麦面粉搅拌成团,在35 ℃发酵12h;(1) Centrifuge 200 mL of Lactobacillus San Francisco Ls-1001 fermentation broth at 4000 r/min for 4 min to remove the supernatant, dissolve the obtained sludge in 50 mL of purified water, add 200 g of wheat flour and stir Agglomerate and ferment at 35°C for 12 hours;

(2)将第一次发酵好的面团与75 g玉米面混合揉匀捏成碎屑,于35 ℃烘干箱中烘5 h;(2) Mix the dough fermented for the first time with 75 g cornmeal and knead it into crumbs, and bake in a drying oven at 35 °C for 5 h;

(3)将烘干彻底的碎屑放入粉碎机中粉碎,制得酸粉。(3) Put the thoroughly dried debris into a pulverizer to pulverize to obtain acid powder.

表2 酸粉的感官及理化指标Table 2 Sensory and physical and chemical indicators of acid powder

实施例 2:探究不同温度下酸粉的活菌数与水分值Example 2: Exploring the number of viable bacteria and moisture value of acid powder at different temperatures

(1)分别将三份50 g的酸粉碎屑置于30℃、35℃和40℃的烘干箱中烘5 h;(1) Three parts of 50 g crushed acid were dried in ovens at 30°C, 35°C and 40°C for 5 h;

(2)烘好后分别放入粉碎机中粉碎,制得酸粉;(2) After baking, put them into a pulverizer and pulverize them respectively to make acid powder;

(3)测定三份酸粉的活菌数和水分含量。(3) Determination of the number of viable bacteria and moisture content of the three acid powders.

表3 不同温度下烘干酸粉的活菌数与水分值测定Table 3 Determination of viable bacteria count and moisture value of dried acid powder at different temperatures

实施例3:酸粉:小麦面粉=1:5馒头的制备Embodiment 3: the preparation of sour powder:wheat flour=1:5 steamed bread

配料:实施例1制备的酸粉 20 g;小麦面粉100 g;安琪高活性干酵母1.2 g;纯净水60mL。Ingredients: 20 g of sour powder prepared in Example 1; 100 g of wheat flour; 1.2 g of Angel high active dry yeast; 60 mL of purified water.

(1)将 1.2 g安琪高活性干酵母和30 g酸粉溶于60 mL 饮用水,加入100 g小麦面粉搅拌成团;(1) Dissolve 1.2 g of Angel high active dry yeast and 30 g of acid powder in 60 mL of drinking water, add 100 g of wheat flour and stir to form a dough;

(2)将揉匀的面团置于35 ℃的恒温箱中发酵40 min;(2) Put the kneaded dough in an incubator at 35°C to ferment for 40 minutes;

(3)将发酵面团分割成型,蒸制25 min。(3) Divide the fermented dough into shapes and steam for 25 minutes.

感官综合评分:80.25分;Comprehensive sensory score: 80.25 points;

感官评价内容:从外观来看,表皮色泽亮度正常,光滑无褶皱;在内部结构上,气孔较为均匀,回弹慢能复原;口感爽口有嚼劲,尝起来酸味明显;闻着有明显酸味,麦香味较小,无异味。Contents of sensory evaluation: From the appearance, the color and brightness of the skin is normal, smooth and wrinkle-free; in the internal structure, the pores are relatively uniform, and the rebound can be restored slowly; the taste is refreshing and chewy, and the taste is obviously sour; the smell is obvious, The smell of wheat is small and has no peculiar smell.

实施例4:酸粉:小麦面粉=1:6馒头的制备Embodiment 4: the preparation of sour powder:wheat flour=1:6 steamed bread

配料:实施例1制备的酸粉20 g;小麦面粉120 g;安琪活性干酵母1.4 g;纯净水70 mL。Ingredients: 20 g of acid powder prepared in Example 1; 120 g of wheat flour; 1.4 g of Angel active dry yeast; 70 mL of purified water.

本实施例的制作方法与实施例 2 相同。The manufacturing method of this embodiment is the same as that of Embodiment 2.

感官综合评分:86分;Comprehensive sensory score: 86 points;

感官评价内容:从外观上来看,表皮色泽亮度正常,光滑无褶皱;内部结构气孔细小均匀,回弹快能复原;口感较为酸甜,不粘牙,有嚼劲,闻着略有酸味,与老面馒头口感相似;具有浓郁的麦香味,无异味。Sensory evaluation content: From the appearance point of view, the skin color and brightness are normal, smooth and wrinkle-free; the internal structure pores are small and uniform, and the rebound can be restored quickly; the taste is relatively sweet and sour, not sticky to the teeth, chewy, and smells slightly sour. Laomian steamed buns have a similar taste; they have a strong wheat flavor and no peculiar smell.

实施例5:酸粉:小麦面粉=1:7馒头的制备Embodiment 5: the preparation of sour powder:wheat flour=1:7 steamed bread

配料:实施例1制备的酸粉20 g;小麦面粉140 g;安琪活性干酵母1.6 g;纯净水80 mL。Ingredients: 20 g of acid powder prepared in Example 1; 140 g of wheat flour; 1.6 g of Angel active dry yeast; 80 mL of purified water.

本实施例的制作方法与实施例 2 相同。The manufacturing method of this embodiment is the same as that of Embodiment 2.

感官综合评分:80分;Comprehensive sensory score: 80 points;

感官评价内容:从外观上来看,表皮色泽亮度正常,光滑无褶皱;内部结构气孔较为细小,回弹快能复原;口感不粘牙有嚼劲;几乎没有酸味,具有淡淡的麦香味,无异味。Sensory evaluation content: From the appearance point of view, the skin color and brightness are normal, smooth and wrinkle-free; the internal structure pores are relatively small, and the rebound can be restored quickly; the taste is not sticky and chewy; almost no sour taste, with a light wheat fragrance and no peculiar smell .

对比例 1:本对比例是添加老面的馒头的制作方法(老面选自山西忻州)Comparative example 1: This comparative example is the production method of steamed buns with old noodles (old noodles are selected from Xinzhou, Shanxi)

配料:小麦粉120 g;老面25 g;纯净水60 mL。Ingredients: 120 g of wheat flour; 25 g of old noodles; 60 mL of purified water.

(1)将25 g老面溶于60 mL、30℃的纯净水中直至泡软;(1) Dissolve 25 g of old noodles in 60 mL of purified water at 30°C until softened;

(2)向泡软的老面中加入60 g面粉于发酵箱中进行第一次发酵;(2) Add 60 g of flour to the soaked old noodles and carry out the first fermentation in the fermentation box;

(3)向一次发酵后的面糊中加入60 g面粉并将其揉匀;(3) Add 60 g of flour to the batter after primary fermentation and knead it evenly;

(4)揉匀后置于35 ℃的恒温箱中发酵70 min;(4) After kneading well, put it in a constant temperature box at 35 ℃ to ferment for 70 minutes;

(5)将发酵面团分割成型,蒸制25 min。(5) Divide the fermented dough into shapes and steam for 25 minutes.

感官综合评分:85.5分;Comprehensive sensory score: 85.5 points;

感官评价内容:从外观上来看,表皮色泽亮度正常,光滑无褶皱;内部结构气孔细小均匀,回弹快能复原;口感较为酸甜,不粘牙,有嚼劲,闻起来有明显酸味和浓郁的麦香,无异味。Sensory evaluation content: From the appearance point of view, the skin color and brightness are normal, smooth and wrinkle-free; the internal structure has small and uniform pores, and the rebound can be restored quickly; the taste is relatively sweet and sour, not sticky to the teeth, chewy, and it smells obviously sour and rich The wheat fragrance, no peculiar smell.

对比例 2:本对比例是不添加旧金山乳杆菌Ls-1001的馒头的制作方法。Comparative example 2: This comparative example is a method for making steamed buns without adding Lactobacillus San Francisco Ls-1001.

配料:小麦粉140 g;安琪活性干酵母1.4 g;纯净水70 mL。Ingredients: 140 g of wheat flour; 1.4 g of Angel active dry yeast; 70 mL of purified water.

(1)将1.4 g安琪高活性干酵母和溶于70 mL饮用水,加入140 g小麦面粉搅拌成团;(1) Dissolve 1.4 g of Angel high active dry yeast in 70 mL of drinking water, add 140 g of wheat flour and stir to form a dough;

(2)将揉匀的面团置于35 ℃的恒温箱中发酵40 min;(2) Put the kneaded dough in an incubator at 35°C to ferment for 40 minutes;

(3)将发酵面团分割成型,蒸制25 min。(3) Divide the fermented dough into shapes and steam for 25 minutes.

感官综合评分:79.8分;Comprehensive sensory score: 79.8 points;

感官评价内容:在外观上来看,表皮色泽亮度正常,光滑无褶皱;内部结构较为致密;没有酸味,具有淡淡的麦香,无异味,风味平淡。Sensory evaluation content: From the appearance point of view, the skin color and brightness are normal, smooth and wrinkle-free; the internal structure is relatively dense; there is no sour smell, with a light wheat fragrance, no peculiar smell, and the flavor is flat.

Claims (3)

1.一种改善馒头品质的酸粉制备方法,其特征是由以下重量配比的原料制备而成:1. A method for preparing sour powder for improving the quality of steamed bread, characterized in that it is prepared from the raw materials in the following weight proportions: 小麦面粉150~200 g;55~75 g玉米面;旧金山乳杆菌 Ls-1001发酵液150~200 mL(其中菌落数为 2.04×109 CFU/mL)。Wheat flour 150-200 g; cornmeal 55-75 g; Lactobacillus San Francisco Ls-1001 fermentation broth 150-200 mL (the number of colonies is 2.04×109 CFU/mL). 2.根据权利要求1所述的改善馒头品质的酸粉制备方法,其特征是它的制作包括下述步骤:2. the sour powder preparation method for improving steamed bread quality according to claim 1, is characterized in that its making comprises the steps: (1)将150~200 mL旧金山乳杆菌Ls-1001发酵液于4000 r/min的条件下离心4 min去上清液,得到旧金山乳杆菌 Ls-1001菌泥,将菌泥溶于80~120 mL纯净水并加入150~200 g小麦面粉搅拌成团,放入恒温箱发酵8~12 h;(1) Centrifuge 150-200 mL of Lactobacillus San Francisco Ls-1001 fermentation broth at 4000 r/min for 4 minutes to remove the supernatant to obtain Lactobacillus San Francisco Ls-1001 sludge, which is dissolved in 80-120 Add 150-200 g of wheat flour to 1 mL of pure water and stir to form a dough, put it into a constant temperature box to ferment for 8-12 hours; (2)待其发酵好后向其中加入55~75 g玉米面再次揉匀并将其捏碎,放置到烘干箱中烘干,温度为30~35 ℃,时间5~10 h;(2) After it has been fermented, add 55-75 g of cornmeal to it, knead it evenly again, crush it, and place it in a drying oven for drying at a temperature of 30-35 °C for 5-10 hours; (3)将烘干好的碎屑放入粉碎机粉碎成粉,(3) Put the dried debris into the pulverizer and pulverize into powder, 以上所述的旧金山乳杆菌菌泥的制作方法是:将旧金山乳杆菌Ls-1001按体积分数为1%的接种量于 mMRS 培养基中并将其放入厌氧培养箱培养,培养时间24 h,培养温度35℃,厌氧条件为 N2、CO2、O2,其体积百分比为79:20:1;之后将培养好的旧金山乳杆菌Ls-1001 发酵液在转速为4000 r/min的条件下离心4~5 min,倒去上清液,收集沉淀得到旧金山乳杆菌菌泥。The preparation method of the above-mentioned Lactobacillus San Francisco sludge is as follows: the inoculation amount of Lactobacillus San Francisco Ls-1001 according to the volume fraction is 1% in the mMRS medium and put it into an anaerobic incubator for cultivation, and the cultivation time is 24 h , the culture temperature is 35°C, the anaerobic conditions are N2, CO2, O2, and the volume percentage is 79:20:1; after that, the cultured Lactobacillus San Francisco Ls-1001 fermentation broth is centrifuged at a speed of 4000 r/min After 4-5 minutes, the supernatant was poured off, and the precipitate was collected to obtain Lactobacillus San Francisco sludge. 3.根据权利要求1或2所述的改善馒头品质的酸粉制备方法,其特征是,菌株为旧金山乳杆菌 Ls-1001,保藏单位:中国微生物菌种保藏管理委员会普通微生物中心,保藏地址:北京市朝阳区北辰西路1号院3号中国科学院微生物研究所,保藏日期:2017年6月12日,保藏编号:CGMCC No.14232,分类命名:旧金山乳杆菌Lactobacillus sanfrancisco。3. The method for preparing sour powder for improving the quality of steamed bread according to claim 1 or 2, characterized in that the bacterial strain is Lactobacillus San Francisco Ls-1001, and the depository unit is: General Microbiology Center of China Committee for the Preservation of Microbial Cultures, and the depository address is: Institute of Microbiology, Chinese Academy of Sciences, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, date of deposit: June 12, 2017, deposit number: CGMCC No.14232, classification name: Lactobacillus sanfrancisco.
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