CN110089651A - A kind of production method of capsicum leaf enzyme beverage - Google Patents
A kind of production method of capsicum leaf enzyme beverage Download PDFInfo
- Publication number
- CN110089651A CN110089651A CN201910417991.6A CN201910417991A CN110089651A CN 110089651 A CN110089651 A CN 110089651A CN 201910417991 A CN201910417991 A CN 201910417991A CN 110089651 A CN110089651 A CN 110089651A
- Authority
- CN
- China
- Prior art keywords
- enzyme
- capsicum leaf
- capsicum
- production method
- pepper
- Prior art date
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- 235000002566 Capsicum Nutrition 0.000 title claims abstract description 122
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- 238000000855 fermentation Methods 0.000 claims abstract description 30
- 230000004151 fermentation Effects 0.000 claims abstract description 30
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 24
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- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 claims description 4
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/72—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
- A23L2/74—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration using membranes, e.g. osmosis, ultrafiltration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
- A23L21/25—Honey; Honey substitutes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/31—Leuconostoc
- A23V2400/325—Pseudomesenteroides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Water Supply & Treatment (AREA)
- Mycology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
辣椒叶资源量大、营养价值高,但是却一直未得到高效高值利用。本发明提供一种辣椒叶酵素饮料的生产方法,其技术方案包括原料的预处理、混合原料打浆与均质、混合酵素菌剂接种液的制备、辣椒叶原浆酵素发酵、辣椒叶酵素饮料的调配等步骤。本发明通过以红茶菌为核心菌剂复配米曲霉、乳酸菌和酵母菌作为辣椒酵素饮料的混合菌群,实现以辣椒叶为主的植物酵素发酵的精准控制,生产过程简单易控制,安全不易污染,而且原料利用更加充分,为最大限度保持辣椒叶的活性成分及开发高附加值新产品提供了新的途径,产业化前景广阔。Pepper leaves have a large amount of resources and high nutritional value, but they have not been utilized efficiently and with high value. The invention provides a production method of capsicum leaf enzyme beverage, and its technical scheme includes the pretreatment of raw materials, the beating and homogenization of mixed raw materials, the preparation of mixed enzyme bacterial agent inoculum, the fermentation of capsicum leaf puree enzyme, and the production of capsicum leaf enzyme beverage. deployment and other steps. The present invention uses kombucha as the core bacterial agent to compound Aspergillus oryzae, lactic acid bacteria and yeast as the mixed flora of capsicum enzyme beverage, so as to realize the precise control of plant enzyme fermentation mainly composed of capsicum leaves, the production process is simple and easy to control, and it is safe and difficult Pollution, and more fully utilization of raw materials, provides a new way for maintaining the active ingredients of pepper leaves to the maximum and developing new products with high added value, and has broad prospects for industrialization.
Description
【技术领域】【Technical field】
本发明属于发酵饮料生产领域,特别涉及一种辣椒叶酵素饮料的生产方法。The invention belongs to the field of fermented beverage production, in particular to a production method of capsicum leaf enzyme beverage.
【背景技术】【Background technique】
我国是世界上辣椒栽培面积最大的国家,同时也是全球最大的辣椒消费国和出口国。辣椒叶占辣椒植株的绝大部分,因此辣椒叶资源十分丰富。但是一直以来都被视为农业废弃物,对于它的处理大多为焚烧、废弃等,既不利于环境的保护,又忽略了辣椒叶的应用价值。而随着近年来人们对绿色天然食品的青睐,辣椒叶成为蔬菜中的新秀在国外开始流行,东南亚、日本以及我国的香港、澳门等地利用辣椒叶开发营养保健食品。研究表明,辣椒叶的营养成分优于辣椒果实。辣椒叶中总淀粉含量在46.95%以上,总膳食纤维含量接近40%,蛋白质含量可高达21.07%,另外还含有丰富的微量元素、黄酮类物质、脂肪、碳水化合物、VC等。国外的一些研究学者对辣椒叶的营养保健作用进行了一系列的研究。发现辣椒叶具有抗氧化、抗过敏和抗肿瘤等多种活性。由此可见,辣椒叶是集食用、药用和保健于一体的天然食品。my country is the country with the largest pepper cultivation area in the world, and it is also the world's largest pepper consumer and exporter. Pepper leaves account for the vast majority of pepper plants, so pepper leaves are very rich in resources. However, it has always been regarded as agricultural waste, and its treatment is mostly incineration, discarding, etc., which is not conducive to environmental protection, and ignores the application value of pepper leaves. And along with people in recent years to the favor of green natural food, capsicum leaf becomes the rookie in the vegetable and begins to be popular abroad, ground such as Southeast Asia, Japan and my country's Hong Kong, Macao utilizes capsicum leaf to develop nutrition and health food. Studies have shown that the nutritional content of pepper leaves is better than that of pepper fruits. The total starch content in pepper leaves is above 46.95%, the total dietary fiber content is close to 40%, and the protein content can be as high as 21.07%. In addition, it is also rich in trace elements, flavonoids, fat, carbohydrates, VC, etc. Some foreign researchers have conducted a series of studies on the nutritional and health effects of pepper leaves. Capsicum leaves were found to have various activities such as antioxidant, antiallergic and antitumor. It can be seen that capsicum leaf is a natural food integrating edible, medicinal and health care.
鉴于辣椒叶丰富的营养及保健活性,辣椒叶被越来越多的加工成具有保健功能的产品。专利ZL 201310431059.1和201710604676.5将辣椒叶制成茶叶;专利201510813460.0则以新鲜辣椒叶和桑叶的提取液为原料制成具有抗氧化、保护视力、预防辐射、降血脂、降血糖的植物叶保健饮料功效;专利201210267362.8则是提出了一种果蔬复合茶饮料及其制备方法,该饮料具有清热利尿、健脾除湿、生津养胃功效;专利201510771152.6将辣椒叶提取液与蜂蜜粉调配制成具有抗癌防癌、减肥美容、提神醒脑、利肠通便及滋肝润肺降压等功能的保健饮料。但是,这种将辣椒叶直接冲泡饮用的产品显然无法充分地利用辣椒叶中的活性成分,必然造成辣椒叶资源的浪费。也有将生物技术应用于辣椒叶产品生产的报道。专利201711405275.3以乳酸发酵辣椒叶,可以去除辣椒叶的生涩味,增加小分子的香味和营养成分,所制得的辣椒叶茶口感更加醇厚,茶汤香浓,色泽黄绿。专利201410749185.6则是以金花菌发酵辣椒叶或辣椒叶提取液,制成具有独特香气和新颖口感的新型保健茶叶或饮品。专利201610373683.4和201711269524.0以乳酸菌和酵母菌腌制辣椒叶,增加发酵辣椒叶的天然香味,并可抑制杂菌生长,减少食盐及亚硝酸盐含量。但是,这些方法都还较为低端和低值,限制了辣椒叶产业链的发展。In view of the rich nutrition and health-care activity of pepper leaves, pepper leaves are increasingly processed into products with health-care functions. Patents ZL 201310431059.1 and 201710604676.5 make pepper leaves into tea leaves; patent 201510813460.0 uses the extracts of fresh pepper leaves and mulberry leaves as raw materials to make a plant leaf health drink with anti-oxidation, eye protection, radiation prevention, blood fat and blood sugar reduction Patent 201210267362.8 proposes a compound fruit and vegetable tea drink and its preparation method. The drink has the functions of clearing away heat and diuresis, invigorating the spleen and dehumidifying, promoting body fluid and nourishing the stomach; It is a health drink with the functions of treating cancer, losing weight and beautifying, refreshing the mind, invigorating the intestines and defecating, nourishing the liver, nourishing the lungs and lowering blood pressure. But this product that the capsicum leaf is directly brewed for drinking obviously cannot fully utilize the active ingredient in the capsicum leaf, will inevitably cause the waste of capsicum leaf resource. There are also reports on the application of biotechnology to the production of pepper leaf products. Patent 201711405275.3 uses lactic acid to ferment capsicum leaves, which can remove the jerky taste of capsicum leaves, increase the aroma and nutritional components of small molecules, and the prepared capsicum leaf tea has a more mellow taste, rich tea soup, and yellow-green color. Patent 201410749185.6 is a new type of health-care tea or drink with unique aroma and novel taste made by fermenting capsicum leaves or capsicum leaf extracts with golden flower fungus. Patents 201610373683.4 and 201711269524.0 use lactic acid bacteria and yeast to pickle pepper leaves to increase the natural aroma of fermented pepper leaves, inhibit the growth of miscellaneous bacteria, and reduce the content of salt and nitrite. However, these methods are relatively low-end and low-value, which limits the development of the pepper leaf industry chain.
近年来,酵素产品风靡全球,各类酵素的研究开发层出不穷。酵素分为自然菌种发酵和人工菌种发酵两种。后者主要是应用一种或多种已知有益微生物菌种来进行酵素的发酵,常用的发酵菌种有酵母菌、醋酸菌和乳酸菌等。食用植物酵素是以一种或多种新鲜蔬菜、水果、谷豆类、海藻类、药食两用本草类等食材为原料,经多种有益菌长时间发酵而生产的功能性微生物发酵产品。因微生物自身的生长和新陈代谢,实现了物质间的代谢转化,因而也产生了大量具有多种生物活性的酶类和复杂代谢产物或中间代谢产物。也因此酵素通常都具有促进消化吸收、调节人体机能、提高机体免疫力等保健功能。但尚未见到以辣椒叶开发酵素产品的报道。In recent years, enzyme products have become popular all over the world, and research and development of various enzymes have emerged in an endless stream. Enzyme is divided into natural strain fermentation and artificial strain fermentation. The latter mainly uses one or more known beneficial microbial strains to ferment the enzyme. Commonly used fermentation strains include yeast, acetic acid bacteria and lactic acid bacteria. Edible plant enzymes are functional microbial fermented products produced from one or more fresh vegetables, fruits, grains, beans, seaweeds, medicinal and edible herbs, etc., after long-term fermentation of various beneficial bacteria. Due to the growth and metabolism of the microorganism itself, the metabolic transformation between substances has been realized, and a large number of enzymes and complex metabolites or intermediate metabolites with various biological activities have also been produced. Therefore, enzymes usually have health functions such as promoting digestion and absorption, regulating human body functions, and improving body immunity. But do not see the report of fermenting product with capsicum leaf as yet.
红茶菌(Kombucha,tea mushroom或者tea fungus),也俗称“海宝”“胃宝”等,是一种通过醋酸菌、乳酸菌和酵母菌等组成的“共生菌落”发酵茶糖水而成的富含益生生物及活性成分的产品。红茶菌中微生物之间形成了强大共生关系及复杂代谢通路。其中酵母菌是“启动子”和桥梁,在发酵的初级阶段(0-3d)将蔗糖等分解为葡萄糖和果糖,并进一步生成乙醇和CO2;醋酸菌利用葡萄糖和果糖为碳源开始快速生长繁殖,并生成乙酸、葡萄糖酸等代谢产物;乳酸菌利用酵母菌代谢产生的维生素和氨基酸等物质逐渐生长繁殖,并产生一定量的乳酸。乙酸、乙醇的存在可以保护发酵系统免受其它菌株的侵染。越来越多的研究表明红茶菌发酵可以产生大量的活性成分,主要包括葡萄糖酸、醋酸、葡萄糖、果糖、蛋白质、氨基酸、维生素、微量元素、茶多酚、咖啡因、乙醇和二氧化碳等,也因此红茶菌对多种疾病都具有潜在的治疗效果,包括抗氧化,抗致癌,抗糖尿病,提高免疫力,增强肝脏解毒能力,有助于胃溃疡和高胆固醇的治疗等。Kombucha (Kombucha, tea mushroom or tea fungus), also commonly known as "Haibao", "Weibao", etc., is a kind of probiotic-rich tea fermented by a "symbiotic colony" composed of acetic acid bacteria, lactic acid bacteria and yeast. Products with biological and active ingredients. A strong symbiotic relationship and complex metabolic pathways are formed among the microorganisms in kombucha. Among them, yeast is the "promoter" and bridge, which decomposes sucrose into glucose and fructose in the primary stage of fermentation (0-3d), and further produces ethanol and CO 2 ; acetic acid bacteria use glucose and fructose as carbon sources to start rapid growth Propagate and generate metabolites such as acetic acid and gluconic acid; lactic acid bacteria use vitamins and amino acids produced by yeast metabolism to gradually grow and reproduce, and produce a certain amount of lactic acid. The presence of acetic acid and ethanol can protect the fermentation system from other strains. More and more studies have shown that kombucha fermentation can produce a large number of active ingredients, mainly including gluconic acid, acetic acid, glucose, fructose, protein, amino acid, vitamins, trace elements, tea polyphenols, caffeine, ethanol and carbon dioxide, etc. Therefore, kombucha has potential therapeutic effects on various diseases, including anti-oxidation, anti-carcinogenic, anti-diabetic, improving immunity, enhancing liver detoxification ability, and helping the treatment of gastric ulcer and high cholesterol.
本发明的目的在于克服现有辣椒叶利用理念及技术上的不足之处,提供一种辣椒叶酵素饮料的生产方法,为充分发挥辣椒叶中活性成分的功效及开发高附加值新产品提供新途径。The purpose of the present invention is to overcome the deficiencies in the existing concept and technology of pepper leaf utilization, to provide a production method of pepper leaf enzyme beverage, and to provide a new method for fully exerting the efficacy of active ingredients in pepper leaves and developing new products with high added value. way.
【发明内容】【Content of invention】
辣椒叶资源量大、营养价值高,但是却一直未得到高效高值利用。本发明突破现有辣椒叶利用方式中利用率低、开发技术低端、产品价值不高等问题,提供一种辣椒叶酵素饮料的生产方法,为充分发挥辣椒叶中活性成分的功效及开发高附加值新产品提供新途径。Pepper leaves have a large amount of resources and high nutritional value, but they have not been utilized efficiently and with high value. The invention breaks through the problems of low utilization rate, low-end development technology, and low product value in the existing pepper leaf utilization methods, and provides a production method of pepper leaf enzyme beverage, which is to fully exert the efficacy of active ingredients in pepper leaves and develop high-additional beverages. Provide new ways to value new products.
本发明的技术方案如下:Technical scheme of the present invention is as follows:
本发明所述的一种辣椒叶酵素饮料的生产方法包括,原料的预处理、混合原料打浆与均质、混合酵素菌剂接种液的制备、辣椒叶原浆酵素发酵、辣椒叶酵素饮料的调配等步骤。具体的通过以下步骤实现:The production method of a kind of capsicum leaf enzyme beverage of the present invention comprises, the pretreatment of raw material, the beating and homogenization of mixed raw material, the preparation of mixed enzyme bacterial agent inoculum, the fermenting of capsicum leaf protoplasmic enzyme, the deployment of capsicum leaf ferment beverage and other steps. Specifically, it is realized through the following steps:
1)原料预处理:选取无黑斑、枯斑、缺口、虫卵的新鲜辣椒叶,用无菌水轻轻冲洗除去除辣椒叶表面的尘土,常温悬挂晾干表面水分,将洗净的辣椒叶在90℃热水中热烫10s,其他辅料用无菌水冲洗干净,并晾干表面水分;1) Raw material pretreatment: select fresh pepper leaves without black spots, dead spots, gaps, and insect eggs, gently rinse with sterile water to remove the dust on the surface of pepper leaves, hang at room temperature to dry the surface moisture, and wash the pepper leaves The leaves are blanched in hot water at 90°C for 10 seconds, and other auxiliary materials are rinsed with sterile water, and the surface moisture is dried;
2)混合原料打浆与均质:取预处理后的辣椒叶与辅料按照一定重量比混合后,加水进行打浆、均质后得到辣椒叶酵素发酵的原浆;2) beating and homogenization of mixed raw materials: after taking the pretreated pepper leaves and auxiliary materials and mixing them according to a certain weight ratio, adding water for beating and homogenizing to obtain the original pulp of pepper leaf enzyme fermentation;
3)混合酵素菌剂接种液的制备:按照重量体积比1:50-200(w/v,g/ml)将茶叶加入90℃-100℃热水中浸提20min,过滤取茶汤;茶汤倒入已灭菌的容器中,再按照重量体积比5%~20%(wt%)加入食用糖,搅拌充分后接种混合酵素菌剂,以纱布密封,在28-35℃以180rpm振荡培养2-3d后,在纱布上覆盖塑料布密封,在28-35℃静置培养3-5d;3) Preparation of the inoculum of the mixed enzyme bacterial agent: according to the weight-to-volume ratio of 1:50-200 (w/v, g/ml), add tea leaves to 90°C-100°C hot water for 20 minutes, filter the tea soup; pour the tea soup Put it into a sterilized container, add edible sugar according to the weight-to-volume ratio of 5% to 20% (wt%), inoculate the mixed enzyme bacterial agent after stirring fully, seal it with gauze, and cultivate it with 180rpm vibration at 28-35°C for 2- After 3 days, cover the gauze with plastic cloth to seal, and culture at 28-35°C for 3-5 days;
4)辣椒叶原浆酵素发酵:均质的辣椒叶与辅料原浆20~50%(重量体积比,下同);食用糖5~20%,混合酵素菌剂接种液10%~30%,余量无菌纯净水,搅拌均匀后,以纱布密封,在28-35℃以180rpm振荡培养2-5d后,在纱布上覆盖塑料布密封,在28-35℃静置培养5-30d;4) Enzyme fermentation of pepper leaf puree: homogeneous pepper leaf and auxiliary material puree 20-50% (weight to volume ratio, the same below); edible sugar 5-20%, mixed enzyme bacterial agent inoculum 10%-30%, The remaining amount of sterile purified water, after stirring evenly, is sealed with gauze, shake culture at 28-35°C at 180rpm for 2-5d, cover the gauze with plastic cloth and seal, and culture at 28-35°C for 5-30d;
5)辣椒叶酵素饮料的调配:辣椒叶酵素发酵产物过滤,取澄清透明的酵素发酵液,然后加入0.05-0.2%甜味剂、5%蜂蜜、0.1~0.2%果胶、0.01%-0.05%的增香剂,搅拌均匀后静置24h以上,然后以0.2-0.45μm膜过滤后灌入洁净玻璃瓶,得到辣椒叶提取物酵素饮料。5) Preparation of capsicum leaf enzyme beverage: filter the fermented product of capsicum leaf enzyme, take clear and transparent fermented liquid, then add 0.05-0.2% sweetener, 5% honey, 0.1-0.2% pectin, 0.01%-0.05% After stirring evenly, let it stand for more than 24 hours, then filter it with a 0.2-0.45 μm membrane and pour it into a clean glass bottle to obtain a capsicum leaf extract enzyme beverage.
步骤1)中所述的辅料包括菌菇、百合、菠萝、木瓜中的一种或几种。其中菌菇优选香菇和杏鲍菇。辅料优选香菇与菠萝。The auxiliary materials described in step 1) include one or more of mushrooms, lily, pineapple and papaya. Wherein mushroom is preferably shiitake mushroom and king eryngii. The auxiliary materials are preferably shiitake mushrooms and pineapple.
步骤2)中所述的混合原料中辣椒叶的重量占50-80%、辅料的重量占5~20%。优选的辣椒叶重量为65%,辅料中香菇和菠萝的重量分别为20%和15%。In the mixed raw material described in step 2), the weight of capsicum leaves accounts for 50-80%, and the weight of auxiliary materials accounts for 5-20%. The preferred weight of pepper leaves is 65%, and the weight of shiitake mushrooms and pineapples in the auxiliary materials are respectively 20% and 15%.
所述的食用糖包括白砂糖、冰糖、红糖、绵白糖、蜂蜜、浓缩甘蔗汁中的一种或几种的混合物。所述的食用糖添加浓度是指几种糖的总浓度,当添加一种以上的糖时,每种糖的添加量相同。The edible sugar includes one or a mixture of white granulated sugar, rock sugar, brown sugar, soft white sugar, honey and concentrated sugarcane juice. The added concentration of edible sugar refers to the total concentration of several sugars. When more than one kind of sugar is added, the added amount of each sugar is the same.
所述的茶叶包括绿茶、红茶、黑茶中的一种。The tea leaves include one of green tea, black tea and dark tea.
所述的混合酵素菌剂是以红茶菌为核心菌剂,单独或者复配米曲霉、乳酸菌、酵母菌中的一种或者几种。前述的红茶菌母液为市售红茶菌,乳酸菌为植物乳杆菌、假肠膜明串珠菌、嗜酸乳杆菌中的一种或几种,酵母菌为酿酒酵母、异常汉逊酵母和拜耳接合酵母中的一种或多种。The mixed enzyme agent uses kombucha as the core agent, alone or compounded with one or more of Aspergillus oryzae, lactic acid bacteria and yeast. The aforementioned kombucha mother liquor is commercially available kombucha, the lactic acid bacteria are one or more of Lactobacillus plantarum, Leuconostoc pseudoenteritis, and Lactobacillus acidophilus, and the yeasts are Saccharomyces cerevisiae, Hansenula anomaly and Zygomyces bayerii one or more of.
前述的酵素菌剂接种物的接种体积为辣椒叶原浆液体积的20%~50%。The inoculum volume of the aforesaid ferment bacterial agent inoculum is 20%-50% of the volume of the capsicum leaf stock solution.
所述的甜味剂是安赛蜜、甜菊糖中的一种或两种的组合物。The sweetener is one or a combination of acesulfame potassium and stevioside.
所述的增香剂是乙基麦芽酚、香兰素、食品用香精的一种或几种的组合物。The flavor enhancer is one or a combination of ethyl maltol, vanillin and food essence.
【本发明的优点】[Advantages of the present invention]
本发明具有如下优点:The present invention has the following advantages:
1)传统的酵素自然发酵过程难以精准控制,本发明利用红茶菌混合菌群作为核心菌剂,通过复配米曲霉、乳酸菌和酵母菌等制定混合酵素菌群,不仅生产过程简单易控制,安全不易污染,而且原料利用更加充分,为最大限度保持辣椒叶的活性成分及开发高附加值新产品提供了新的途径,产业化前景广阔。1) It is difficult to accurately control the traditional natural fermentation process of enzymes. The present invention uses the kombucha mixed flora as the core bacterial agent, and formulates the mixed enzyme flora by compounding Aspergillus oryzae, lactic acid bacteria and yeast. The production process is not only simple and easy to control, but also safe It is not easy to pollute, and the raw materials are more fully utilized, which provides a new way for maintaining the active ingredients of pepper leaves to the maximum and developing new products with high added value, and has broad prospects for industrialization.
2)本发明的植物酵素饮料是以辣椒叶为主料辅以菌菇、菠萝、木瓜等,原料本身营养价值较高,同时还含有包括漆酶、纤维素酶、蛋白酶等丰富的酶,使得酵素产品的营养价值更高。有益菌共生发酵促进了酵素发酵体系中有益代谢产物的大量产生,从而使得所制备的辣椒叶提取物酵素饮料具有特殊的生物活性。2) The plant enzyme beverage of the present invention is based on capsicum leaves and supplemented with mushrooms, pineapples, papaya, etc. The nutritional value of the raw material itself is relatively high, and it also contains abundant enzymes including laccase, cellulase, protease, etc., so that Enzyme products have higher nutritional value. The symbiotic fermentation of beneficial bacteria promotes the production of a large number of beneficial metabolites in the enzyme fermentation system, so that the prepared capsicum leaf extract enzyme beverage has special biological activity.
【具体实施方法】【Specific implementation method】
下面结合具体实施例对本发明做进一步说明,但并非穷举,本领域技术人员不经过创造性劳动的简单复制和改进均属于本发明权利要求保护的范围。The present invention will be further described below in conjunction with specific embodiments, but it is not exhaustive. Simple duplication and improvement by those skilled in the art without creative work all belong to the protection scope of the claims of the present invention.
实施例1Example 1
选取无黑斑、枯斑、缺口、虫卵的新鲜辣椒叶,用无菌水轻轻冲洗除去除辣椒叶表面的尘土,常温悬挂晾干表面水分,将洗净的辣椒叶在90℃热水中热烫10s,辅料香菇、菠萝、百合用无菌水冲洗干净,并晾干表面水分;取预处理后的辣椒叶50份与20份香菇和15份菠萝和15份百合混合后,加水进行打浆、均质后得到辣椒叶酵素发酵的原浆液。Select fresh pepper leaves without black spots, dead spots, gaps, and insect eggs, gently rinse with sterile water to remove the dust on the surface of the pepper leaves, hang at room temperature to dry the surface water, and put the washed pepper leaves in hot water at 90°C Blanch in medium heat for 10s, rinse the auxiliary materials mushroom, pineapple and lily with sterile water, and dry the surface moisture; take 50 parts of pretreated pepper leaves, mix with 20 parts of mushroom, 15 parts of pineapple and 15 parts of lily, add water for After beating and homogenizing, the original puree liquid fermented by capsicum leaf enzyme is obtained.
按照重量体积比1:50(w/v,g/ml)将绿茶加入90℃-100℃热水中浸提20min,过滤取茶汤;茶汤倒入已灭菌的容器中,按照重量体积比15%(wt%)加入白砂糖,搅拌充分后接种混合酵素菌剂:红茶菌母液、假肠膜明串珠菌CICC 21115(Leuconostocpseudomesenteroides)、米曲霉CICC 40620(Aspergillus oryzae,下同)、酿酒酵母CICC1251(Saccharomyces cerevisiae,下同)、拜耳接合酵母CICC 32677(Zygosaccharomycesbailii,下同),以纱布密封,在30℃以180rpm振荡培养2-3d后,在纱布上覆盖塑料布密封,在30℃静置培养3-5d。According to the weight-volume ratio of 1:50 (w/v, g/ml), add green tea into hot water at 90°C-100°C to extract for 20 minutes, filter the tea soup; pour the tea soup into a sterilized container, % (wt%) white granulated sugar was added, stirred fully and inoculated with mixed enzyme bacterial agents: Kombucha mother liquor, Leuconostocpseudomesenteroides CICC 21115 (Leuconostocpseudomesenteroides), Aspergillus oryzae CICC 40620 (Aspergillus oryzae, the same below), Saccharomyces cerevisiae CICC1251 ( Saccharomyces cerevisiae, the same below), Zygosaccharomycesbailii CICC 32677 (Zygosaccharomycesbailii, the same below), sealed with gauze, shaking culture at 180rpm at 30°C for 2-3d, covered with plastic cloth on the gauze, sealed, and cultured statically at 30°C for 3 days -5d.
按照辣椒叶酵素发酵原浆液20%(wt%,下同)、食用糖5%、混合酵素菌剂接种液10%~30%,余量用无菌纯净水,搅拌均匀后,以纱布密封,在30℃以180rpm振荡培养2d后,在纱布上覆盖塑料布密封,在30℃静置培养7d后,过滤取澄清透明的酵素发酵液,然后加入0.05%甜菊糖、5%蜂蜜、0.1%果胶、0.01%的香兰素,搅拌均匀后静置24h以上,然后以0.2-0.45μm膜过滤后灌入洁净玻璃瓶,得到辣椒叶提取物酵素饮料。According to 20% (wt%, the same below) of capsicum leaf enzyme fermentation stock solution, 5% edible sugar, 10% to 30% of mixed enzyme inoculum inoculum, and sterile purified water for the remainder, after stirring evenly, seal it with gauze, After vibrating at 30°C and 180rpm for 2 days, cover the gauze with plastic cloth and seal it. After standing and culturing at 30°C for 7 days, filter the clear and transparent enzyme fermentation liquid, and then add 0.05% stevioside, 5% honey, and 0.1% fruit. Glue and 0.01% vanillin are stirred evenly and left to stand for more than 24 hours, then filtered through a 0.2-0.45 μm membrane and poured into a clean glass bottle to obtain a capsicum leaf extract enzyme beverage.
实施例2Example 2
选取无黑斑、枯斑、缺口、虫卵的新鲜辣椒叶,用无菌水轻轻冲洗除去除辣椒叶表面的尘土,常温悬挂晾干表面水分,将洗净的辣椒叶在90℃热水中热烫10s,辅料菠萝、木瓜用无菌水冲洗干净,并晾干表面水分;取预处理后的辣椒叶65份与15份菠萝和20份木瓜混合后,加水进行打浆、均质后得到辣椒叶酵素发酵的原浆液。Select fresh pepper leaves without black spots, dead spots, gaps, and insect eggs, gently rinse with sterile water to remove the dust on the surface of the pepper leaves, hang at room temperature to dry the surface water, and put the washed pepper leaves in hot water at 90°C Medium-heat blanching for 10s, the auxiliary materials pineapple and papaya are rinsed with sterile water, and the surface moisture is dried; take 65 parts of pretreated pepper leaves, mix with 15 parts of pineapple and 20 parts of papaya, add water for beating, and homogenize to obtain The original puree of capsicum leaf enzyme fermentation.
按照重量体积比1:100(w/v,g/ml)将红茶加入90℃-100℃热水中浸提20min,过滤取茶汤;茶汤倒入已灭菌的容器中,按照重量体积比10%(wt%)加入白砂糖和红糖的混合物,搅拌充分后接种混合酵素菌剂:红茶菌母液、植物乳杆菌植物亚种CICC 20272(Lactobacillus plantarum subsp.Plantarum,下同)、米曲霉、拜耳接合酵母、异常汉逊酵母CICC 1312(Hansenula anomala var.anomala,下同),以纱布密封,在28℃以180rpm振荡培养3d后,在纱布上覆盖塑料布密封,在28℃静置培养5d。According to the weight-volume ratio of 1:100 (w/v, g/ml), add black tea into hot water at 90°C-100°C for 20 minutes, filter the tea soup; % (wt%) Add the mixture of white sugar and brown sugar, stir well and then inoculate the mixed enzyme agent: Kombucha mother liquor, Lactobacillus plantarum subsp. Plantarum CICC 20272 (Lactobacillus plantarum subsp. Yeast, Hansenula anomala CICC 1312 (Hansenula anomala var.anomala, the same below), sealed with gauze, cultured at 28°C with shaking at 180rpm for 3 days, covered with plastic cloth on the gauze, sealed, and cultured statically at 28°C for 5 days.
按照辣椒叶酵素发酵原浆液40%(wt%,下同)、浓缩甘蔗汁10%、混合酵素菌剂接种液20%,余量用无菌纯净水,搅拌均匀后,以纱布密封,在28℃以180rpm振荡培养5d后,在纱布上覆盖塑料布密封,在30℃静置培养14d后,过滤取澄清透明的酵素发酵液,然后加入0.1%甜菊糖、5%蜂蜜、0.1%果胶、0.02%的食用香精,搅拌均匀后静置24h以上,然后以0.2-0.45μm膜过滤后灌入洁净玻璃瓶,得到辣椒叶提取物酵素饮料。According to 40% (wt%, the same below) of capsicum leaf enzyme fermentation stock solution, 10% of concentrated sugarcane juice, 20% of mixed enzyme bacterial agent inoculum, the balance is with sterile purified water, after stirring evenly, seal it with gauze, in 28 After shaking and culturing at 180rpm for 5 days, cover the gauze with plastic cloth and seal it. After standing and culturing at 30 degrees for 14 days, filter the clear and transparent enzyme fermentation liquid, and then add 0.1% stevioside, 5% honey, 0.1% pectin, 0.02% food flavor, stir evenly, let it stand for more than 24 hours, then filter it with a 0.2-0.45 μm membrane and pour it into a clean glass bottle to obtain a capsicum leaf extract enzyme beverage.
实施例3Example 3
选取无黑斑、枯斑、缺口、虫卵的新鲜辣椒叶,用无菌水轻轻冲洗除去除辣椒叶表面的尘土,常温悬挂晾干表面水分,将洗净的辣椒叶在90℃热水中热烫10s,辅料菠萝用无菌水冲洗干净,并晾干表面水分;取预处理后的辣椒叶80份与菠萝20份混合后,加水进行打浆、均质后得到辣椒叶酵素发酵的原浆液。Select fresh pepper leaves without black spots, dead spots, gaps, and insect eggs, gently rinse with sterile water to remove the dust on the surface of the pepper leaves, hang at room temperature to dry the surface water, and put the washed pepper leaves in hot water at 90°C Medium-heat blanching for 10 seconds, the accessory pineapple is rinsed with sterile water, and the surface moisture is dried; 80 parts of pretreated pepper leaves are mixed with 20 parts of pineapple, and water is added for beating and homogenization to obtain the raw material of pepper leaf enzyme fermentation. slurry.
按照重量体积比1:100(w/v,g/ml)将绿茶加入90℃-100℃热水中浸提20min,过滤取茶汤;茶汤倒入已灭菌的容器中,按照重量体积比20%(wt%)加入等量的白砂糖和蜂蜜的混合物,搅拌充分后接种混合酵素菌剂:红茶菌母液、植物乳杆菌植物亚种、假肠膜明串珠菌、米曲霉、异常汉逊酵母,以纱布密封,在32℃以180rpm振荡培养3d后,在纱布上覆盖塑料布密封,在32℃静置培养5d。According to the weight-to-volume ratio of 1:100 (w/v, g/ml), add green tea into hot water at 90°C-100°C for 20 minutes, filter the tea soup; pour the tea soup into a sterilized container, % (wt%) Add the same amount of white sugar and honey mixture, stir well and then inoculate the mixed enzyme agent: Kombucha mother liquor, Lactobacillus plantarum subspecies, Leuconostoc pseudoenterolis, Aspergillus oryzae, Hansenula anomalies , sealed with gauze, cultured at 32°C with shaking at 180rpm for 3 days, covered with plastic cloth on the gauze, and cultured statically at 32°C for 5 days.
按照辣椒叶酵素发酵原浆液50%(wt%,下同)、等量的白砂糖和蜂蜜混合物10%、混合酵素菌剂接种液30%,余量用无菌纯净水,搅拌均匀后,以纱布密封,在32℃以180rpm振荡培养5d后,在纱布上覆盖塑料布密封,在32℃静置培养30d后,过滤取澄清透明的酵素发酵液,然后加入0.1%安赛蜜、5%蜂蜜、0.1%果胶、0.02%的乙基麦芽酚,搅拌均匀后静置24h以上,然后以0.2-0.45μm膜过滤后灌入洁净玻璃瓶,得到辣椒叶提取物酵素饮料。According to 50% (wt%, the same below) of capsicum leaf enzyme fermentation stock solution, 10% of the same amount of white sugar and honey mixture, 30% of mixed enzyme inoculum inoculum, and the balance is sterile pure water, after stirring evenly, with Seal it with gauze, shake it at 32°C at 180rpm for 5 days, cover the gauze with plastic cloth and seal it, let it stand at 32°C for 30 days, filter the clear and transparent enzyme fermentation liquid, and then add 0.1% acesulfame potassium and 5% honey , 0.1% pectin, 0.02% ethyl maltol, stir evenly and let it stand for more than 24 hours, then filter it with a 0.2-0.45 μm membrane and pour it into a clean glass bottle to obtain the capsicum leaf extract enzyme beverage.
实施例4Example 4
选取无黑斑、枯斑、缺口、虫卵的新鲜辣椒叶,用无菌水轻轻冲洗除去除辣椒叶表面的尘土,常温悬挂晾干表面水分,将洗净的辣椒叶在90℃热水中热烫10s,辅料香菇、百合用无菌水冲洗干净,并晾干表面水分;取预处理后的辣椒叶50份与香菇25份和百合25份混合后,加水进行打浆、均质后得到辣椒叶酵素发酵的原浆液。Select fresh pepper leaves without black spots, dead spots, gaps, and insect eggs, gently rinse with sterile water to remove the dust on the surface of the pepper leaves, hang at room temperature to dry the surface water, and put the washed pepper leaves in hot water at 90°C Blanch in medium heat for 10s, rinse the auxiliary materials shiitake mushrooms and lilies with sterile water, and dry the surface moisture; take 50 parts of pretreated pepper leaves, mix with 25 parts of shiitake mushrooms and 25 parts of lilies, add water for beating, and homogenize to obtain The original puree of capsicum leaf enzyme fermentation.
按照重量体积比1:100(w/v,g/ml)将绿茶加入90℃-100℃热水中浸提20min,过滤取茶汤;茶汤倒入已灭菌的容器中,按照重量体积比15%(wt%)加入等量的白砂糖、冰糖和蜂蜜的混合物,搅拌充分后接种混合酵素菌剂:红茶菌母液、假肠膜明串珠菌、米曲霉、拜耳接合酵母,以纱布密封,在30℃以180rpm振荡培养2d后,在纱布上覆盖塑料布密封,在30℃静置培养5d。According to the weight-to-volume ratio of 1:100 (w/v, g/ml), add green tea into hot water at 90°C-100°C for 20 minutes, filter the tea soup; pour the tea soup into a sterilized container, % (wt%) adds the mixture of equal amount of white granulated sugar, rock sugar and honey, inoculates mixed ferment bacterial agent after stirring fully: mother liquor of kombucha, Leuconostoc pseudoenterolis, Aspergillus oryzae, Zyggiosaccharomyces bayeri, seal with gauze, in After vibrating at 180 rpm at 30°C for 2 days, the gauze was covered with plastic cloth to seal, and cultured at 30°C for 5 days.
按照辣椒叶酵素发酵原浆液40%(wt%,下同)、等量的红糖和浓缩甘蔗汁的混合物10%、混合酵素菌剂接种液20%,余量用无菌纯净水,搅拌均匀后,以纱布密封,在35℃以180rpm振荡培养5d后,在纱布上覆盖塑料布密封,在35℃静置培养15d后,过滤取澄清透明的酵素发酵液,然后加入0.05%安赛蜜和0.05%甜菊糖、5%蜂蜜、0.2%果胶、0.01%的食用香精,搅拌均匀后静置24h以上,然后以0.2-0.45μm膜过滤后灌入洁净玻璃瓶,得到辣椒叶提取物酵素饮料。According to 40% (wt%, the same below) of capsicum leaf enzyme fermentation stock solution, 10% of the mixture of equal amounts of brown sugar and concentrated sugarcane juice, 20% of mixed enzyme inoculum, and the balance is sterile pure water, after stirring evenly , sealed with gauze, and vibrated at 35°C at 180rpm for 5 days, covered with plastic cloth on the gauze and sealed, and cultured at 35°C for 15 days, filtered to obtain a clear and transparent enzyme fermentation liquid, and then added 0.05% acesulfame potassium and 0.05 % stevioside, 5% honey, 0.2% pectin, 0.01% food essence, stir evenly, let stand for more than 24 hours, then filter with 0.2-0.45 μm membrane and pour into clean glass bottles to obtain capsicum leaf extract enzyme beverage.
实施例5Example 5
选取无黑斑、枯斑、缺口、虫卵的新鲜辣椒叶,用无菌水轻轻冲洗除去除辣椒叶表面的尘土,常温悬挂晾干表面水分,将洗净的辣椒叶在90℃热水中热烫10s,辅料杏鲍菇、木瓜、百合用无菌水冲洗干净,并晾干表面水分;取预处理后的辣椒叶60份与杏鲍菇20份、木瓜10份、百合10份混合后,加水进行打浆、均质后得到辣椒叶酵素发酵的原浆液。Select fresh pepper leaves without black spots, dead spots, gaps, and insect eggs, gently rinse with sterile water to remove the dust on the surface of the pepper leaves, hang at room temperature to dry the surface water, and put the washed pepper leaves in hot water at 90°C Blanch in medium heat for 10s, rinse the auxiliary materials Pleurotus eryngii, papaya, and lily with sterile water, and dry the surface moisture; take 60 parts of pretreated pepper leaves, mix with 20 parts of Pleurotus eryngii, 10 parts of papaya, and 10 parts of lily Finally, add water for beating and homogenization to obtain the original slurry of pepper leaf enzyme fermentation.
按照重量体积比1:50(w/v,g/ml)将红茶加入90℃-100℃热水中浸提20min,过滤取茶汤;茶汤倒入已灭菌的容器中,按照重量体积比20%(wt%)加入等量白砂糖和红糖的混合物,搅拌充分后接种红茶菌母液,以纱布密封,在30℃以180rpm振荡培养2d后,在纱布上覆盖塑料布密封,在30℃静置培养5d。According to the weight-volume ratio of 1:50 (w/v, g/ml), add black tea into hot water at 90°C-100°C to extract for 20 minutes, filter the tea soup; pour the tea soup into a sterilized container, % (wt%) add the mixture of equal amount of white granulated sugar and brown sugar, inoculate the mother liquor of kombucha after stirring fully, seal with gauze, shake and cultivate at 180rpm at 30°C for 2 days, cover the gauze with plastic cloth and seal, and let it stand at 30°C Culture 5d.
按照辣椒叶酵素发酵原浆液50%(wt%,下同)、白砂糖和红糖的1:1混合物5%、红茶菌母液20%,余量用无菌纯净水,搅拌均匀后,以纱布密封,在30℃以180rpm振荡培养5d后,在纱布上覆盖塑料布密封,在30℃静置培养15d后,过滤取澄清透明的酵素发酵液,然后加入0.2%甜菊糖、5%蜂蜜、0.1%果胶、0.02%的食用香精和0.02%的香兰素,搅拌均匀后静置24h以上,然后以0.2-0.45μm膜过滤后灌入洁净玻璃瓶,得到辣椒叶提取物酵素饮料。According to 50% (wt%, the same below) of pepper leaf fermented fermented stock solution, 5% of a 1:1 mixture of white sugar and brown sugar, 20% of kombucha mother liquor, and sterile purified water for the rest, after stirring evenly, seal it with gauze , after shaking and culturing at 30°C at 180rpm for 5 days, cover the gauze with plastic cloth and seal it. After standing and culturing at 30°C for 15 days, filter the clear and transparent enzyme fermentation liquid, and then add 0.2% stevioside, 5% honey, 0.1% Pectin, 0.02% edible essence and 0.02% vanillin are stirred evenly and left to stand for more than 24 hours, then filtered through a 0.2-0.45 μm membrane and poured into a clean glass bottle to obtain the capsicum leaf extract enzyme beverage.
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CN117243315A (en) * | 2023-11-20 | 2023-12-19 | 山东农圣恒昌农业科技有限公司 | Fermented chilli beverage and processing method thereof |
CN117243315B (en) * | 2023-11-20 | 2024-02-02 | 山东农圣恒昌农业科技有限公司 | Fermented chilli beverage and processing method thereof |
CN117391882A (en) * | 2023-12-08 | 2024-01-12 | 山东农圣恒昌农业科技有限公司 | Production method of chilli leaf enzyme beverage |
CN117391882B (en) * | 2023-12-08 | 2024-03-15 | 山东农圣恒昌农业科技有限公司 | Production method of chilli leaf enzyme beverage |
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