CN110082473A - A kind of spot sample device for aflatoxins detection based on thin layer chromatography - Google Patents
A kind of spot sample device for aflatoxins detection based on thin layer chromatography Download PDFInfo
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- CN110082473A CN110082473A CN201910408453.0A CN201910408453A CN110082473A CN 110082473 A CN110082473 A CN 110082473A CN 201910408453 A CN201910408453 A CN 201910408453A CN 110082473 A CN110082473 A CN 110082473A
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- 229930195730 Aflatoxin Natural products 0.000 title claims abstract description 27
- 239000005409 aflatoxin Substances 0.000 title claims abstract description 27
- 238000004809 thin layer chromatography Methods 0.000 title claims abstract description 27
- 238000001514 detection method Methods 0.000 title claims abstract description 26
- 230000007246 mechanism Effects 0.000 claims abstract description 37
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000003381 stabilizer Substances 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims description 19
- 239000007787 solid Substances 0.000 claims description 4
- 238000009826 distribution Methods 0.000 claims description 3
- 239000005357 flat glass Substances 0.000 abstract description 22
- 239000011521 glass Substances 0.000 abstract description 10
- 239000007788 liquid Substances 0.000 description 6
- 238000007639 printing Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 description 3
- 241000228197 Aspergillus flavus Species 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 241000228230 Aspergillus parasiticus Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000011868 grain product Nutrition 0.000 description 2
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/91—Application of the sample
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The spot sample device for aflatoxins detection that the present invention relates to a kind of based on thin layer chromatography, including pedestal, top plate, controller, plate, correction mechanism, point sample mechanism, hopper, two side plates and four stabilizer blades, correction mechanism includes driving assembly and four corrective components, corrective component includes mobile bar, fixed ring and rectification plate, point sample mechanism includes first motor, shaft, circular frame, four sliding blocks, four sunpenders and four point sample components and four connection components, driving assembly includes the second motor, turntable and four drive rods, sheet glass is fixed on the surface of plate by correction mechanism by the spot sample device for aflatoxins detection based on thin layer chromatography, convenient for point sample mechanism, accurately surface carries out point sample on the glass sheet, moreover, automatic point sample is carried out to four sides above sheet glass simultaneously using point sample mechanism, Point sample efficiency is improved, to improve the practicability of equipment.
Description
Technical field
The present invention relates to food inspection equipment field, in particular to a kind of examining for aflatoxins based on thin layer chromatography
The spot sample device of survey.
Background technique
Aflatoxin is the metabolite of the generations such as aspergillus flavus, aspergillus parasiticus.When grain fails to dry and store in time
Not at that time, it tends to generate toxin by aspergillus flavus or aspergillus parasiticus pollution.Aflatoxin is found so far
The maximum mycotoxin of toxicity, being prevalent in aflatoxin in the grain and cereal product of mildew has a variety of detection sides
Method is thin layer chromatography (TLC) wherein the most classical, and principle is to extract sample fastening, is column chromatography, elution, concentration, thin
After layer separation, bluish violet or yellow-green fluorescence are generated under wavelength 365nm ultraviolet lamp tube, and show most on thin layer according to it
Low detected level determines its content, is a kind of half-quantitative detection technology.
It completes TLC analysis to usually require by making sheet, point sample, expansion and detect FOUR EASY STEPS altogether, wherein point sample is to utilize
Microsyringe carries out point sample, before point sample, first uses pencil on the thin-layer tablet made of sheet glass (20cm*20cm) away from end
Gently drawn at 1cm with horizontal line, then draw sample liquid gently point sample on horizontal line with capillary, if will point sample again, have to
Deng preceding point sample remnants solvent volatilization after in point sample, a face point sample least bit is excessive.General spot diameter is greater than 2mm, unsuitable
More than 5mm, distance is 1cm effect, sampling point and glass edge distance at least 1cmm between point, and people pass through manually in glass simultaneously at present
Glass on piece carries out point sample, not only point sample inefficiency, but also the speckle displacement of point sample is easy to appear deviation, and then affects Huang Qu
The TLC of mycin is detected, thus people need it is a kind of being capable of the device of accurate point sample on the glass sheet automatically.
Summary of the invention
The technical problem to be solved by the present invention is for overcome the deficiencies in the prior art, providing a kind of based on tlc analysis
The spot sample device for aflatoxins detection of method.
The technical solution adopted by the present invention to solve the technical problems is: a kind of to be used for aspergillus flavus based on thin layer chromatography
Element detection spot sample device, including pedestal, top plate, controller, plate, correction mechanism, point sample mechanism, hopper, two side plates and
Four stabilizer blades, the side plate are fixed on the top of pedestal, and the top plate is erected on two side plates, and the controller is fixed on it
In on a side plate, PLC is equipped in the controller, the hopper is fixed on the top of top plate, the quadrangle punishment of the plate
The top of pedestal is not fixed on by four stabilizer blades, the correction mechanism is located at the lower section of plate, and the point sample mechanism is located at top
The lower section of plate；
The correction mechanism includes driving assembly and four corrective components, and four corrective components are located at the four of plate
Side, the driving component are located at the underface of plate, and the corrective component includes mobile bar, fixed ring and rectification plate, the drive
One end of dynamic component and mobile bar is sequentially connected, and the rectification plate is fixed on the top of the other end of mobile bar, the fixed ring
It is fixed on the lower section of plate, the Fixing shrink ring is located in mobile bar；
The point sample mechanism includes first motor, shaft, circular frame, four sliding blocks, four sunpenders and four point sample components
With four connection components, the first motor is fixed on the lower section of top plate, and the first motor is electrically connected with PLC, and described first
The transmission connection of the bottom end of motor and shaft, four connection components are circumferentially uniformly distributed the periphery of the bottom end of shaft, the connection group
Part is located at the inside of circular frame, and the both ends of the connection component are connect with two sliding blocks respectively, and the circular frame is parallel with top plate,
The quadrangle distribution of the circular frame is fixed on the lower section of top plate by four sunpenders, and four sliding blocks are respectively sleeved at the four of circular frame
On side, the point sample component and sliding block are corresponded, and the point sample component is located at the lower section of sliding block, the point sample component and
Hopper connection.
Preferably, the driving component includes the second motor, turntable in order to drive four mobile bars while be moved
With four drive rods, second motor is fixed on the lower section of plate, and second motor is electrically connected with PLC, second electricity
Machine and turntable are sequentially connected, and four drive rods are circumferentially evenly distributed on the periphery of turntable, and the drive rod and corrective component are one by one
Corresponding, one end of the drive rod and turntable are hinged, and the other end and mobile bar of the drive rod are hinged.
Preferably, the stable rotation in order to guarantee turntable, the periphery of the turntable is equipped with several sliding shoes, the plate
Lower section be equipped with annular groove, the sliding shoe matches with annular groove, and the sliding shoe is slidably connected with annular groove.
Preferably, the annular groove is dovetail groove in order to further ensure the stable rotation of turntable.
Preferably, for the ease of being slided with movable slider along the side of circular frame by connection component, the connection group
Part includes that connecting rod, connecting tube and two connection units, one end of the connecting rod are fixedly connected with shaft, the connecting rod
It is fixedly connected at the other end and the center of connecting tube, two connection units are located at the both ends of connecting tube, the connection unit
Including slide plate and slide bar, the periphery of the slide plate and the inner wall of connecting tube are tightly connected, and the slide plate and one end with slide bar are solid
Fixed connection, the other end and sliding block of the slide bar are hinged, are equipped at the center of the side of the separate connecting rod of the connecting tube logical
Hole.
Preferably, the connection unit further includes stop collar for the moving direction of fixed slip bar, the stop collar is solid
It is scheduled on the inner wall of connecting tube, the stop collar is set on slide bar.
Preferably, in order to avoid, into ash, the connecting tube is interior to be equipped with strainer, and the filter cover is located at through-hole in connecting tube
Place.
Preferably, for the ease of carry out point sample, the point sample component include dropper, microcap, communicating pipe, sealing plate,
Lifting unit and two vertical bars, two vertical bars are located at the two sides of microcap, and the microcap is fixed on sliding block by vertical bar
Lower section, the lifting unit is located at the lower section of sliding block, and the lifting unit and sealing plate are sequentially connected, the sealing plate it is outer
Week and the inner wall of microcap are tightly connected, and the dropper is fixed on the bottom end of microcap, are equipped with valve, the valve in the dropper
Door is electrically connected with PLC, and the bottom end of the dropper with the bottom of hopper by being connected to communicating pipe.
Preferably, in order to control the lifting moving of sealing plate, the lifting unit includes third motor, screw rod and set
Pipe, the third motor is fixed on the lower section of sliding block, and the third motor is electrically connected with PLC, the third motor and screw rod
The bottom end of top transmission connection, the screw rod is arranged in casing, and described sleeve pipe is fixed on the lower section of sealing plate, described sleeve pipe
It is equipped with and the matched screw thread of screw rod with the junction of screw rod.
Preferably, the driving force in order to guarantee first motor, the first motor is DC servo motor.
The invention has the advantages that should be passed through based on the spot sample device for aflatoxins detection of thin layer chromatography strong
Sheet glass is fixed on the surface of plate by positive mechanism, and convenient for point sample mechanism, accurately surface carries out point sample on the glass sheet, not only
In this way, carrying out automatic point sample to four sides above sheet glass simultaneously using point sample mechanism, point sample efficiency is improved, to improve
The practicability of equipment.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is the structural schematic diagram of the spot sample device for aflatoxins detection of the invention based on thin layer chromatography；
Fig. 2 is the knot of the point sample mechanism of the spot sample device for aflatoxins detection of the invention based on thin layer chromatography
Structure schematic diagram；
Fig. 3 is the knot of the point sample component of the spot sample device for aflatoxins detection of the invention based on thin layer chromatography
Structure schematic diagram；
Fig. 4 is that the structure of the branch pipe of the correction mechanism for aflatoxins detection of the invention based on thin layer chromatography is shown
It is intended to；
In figure: 1. pedestals, 2. top plates, 3. controllers, 4. plates, 5. hoppers, 6. side plates, 7. stabilizer blades, 8. mobile bars, 9. is solid
Determine ring, 10. rectification plates, 11. first motors, 12. circular frames, 13. sliding blocks, 14. sunpenders, 15. second motors, 16. turntables, 17.
Drive rod, 18. sliding shoes, 19. annular grooves, 20. connecting rods, 21. connecting tubes, 22. slide plates, 23. slide bars, 24. stop collars, 25.
Strainer, 26. droppers, 27. microcaps, 28. communicating pipes, 29. sealing plates, 30. vertical bars, 31. third motors, 32. screw rods, 33. sets
Pipe.
Specific embodiment
In conjunction with the accompanying drawings, the present invention is further explained in detail.These attached drawings are simplified schematic diagram, only with
Illustration illustrates basic structure of the invention, therefore it only shows the composition relevant to the invention.
As shown in Figure 1, a kind of spot sample device for aflatoxins detection based on thin layer chromatography, including pedestal 1, top
Plate 2, controller 3, plate 4, correction mechanism, point sample mechanism, 5, two side plates 6 of hopper and four stabilizer blades 7, the side plate 6 are fixed
In the top of pedestal 1, the top plate 2 is erected on two side plates 6, and the controller 3 is fixed therein on a side plate 6, institute
It states and is equipped with PLC in controller 3, the hopper 5 is fixed on the top of top plate 2, and the four corners of the plate 4 pass through four branch respectively
Foot 7 is fixed on the top of pedestal 1, and the correction mechanism is located at the lower section of plate 4, and the point sample mechanism is located at the lower section of top plate 2；
PLC, i.e. programmable logic controller (PLC), it is used for its internally stored program, is held using a kind of programmable memory
The user oriented instruction such as row logical operation, sequential control, timing, counting and arithmetical operation, and by digital or analog defeated
Enter/export the various types of mechanical or production processes of control, its essence is a kind of computer for being exclusively used in Industry Control, hardware
Structure is substantially identical as microcomputer, the reception and output of processing and the instruction of data is generally used for, for realizing in
Centre control.
In the spot sample device, top plate 2 is supported by two side plates 6, the top of top plate 2, by hopper 5 for depositing
The sample liquid of detection aflatoxins content is put, when being detected, machine lamellae, that is, sheet glass is placed on by four stabilizer blades 7 by user
It fixes on the plate 4 of support, equipment operation is then operated by controller 3, the placement location of sheet glass is corrected by correction mechanism,
So that the central axis of plate 4 is overlapped with the central axis of sheet glass, sheet glass is placed exactly in the underface of point sample mechanism at this time,
Then model machine structure in the control point PLC carries out printing operation in the upper surface of sheet glass.
As shown in figure 4, the correction mechanism includes driving assembly and four corrective components, four corrective components are located at
Four sides of plate 4, the driving component are located at the underface of plate 4, and the corrective component includes mobile bar 8, fixed ring 9 and rectifys
One end of positive plate 10, the driving component and mobile bar 8 is sequentially connected, and the rectification plate 10 is fixed on the other end of mobile bar 8
Top, the fixed ring 9 is fixed on the lower section of plate 4, and the fixed ring 9 is set in mobile bar 8；
When correction mechanism is run, PLC controls the driving assembly starting of 4 lower section of plate, while acting on the correction group of four sides
On part, drive mobile bar 8 in four corrective components simultaneously close to or far from driving assembly, using being fixed on 4 lower section of plate
Fixed ring 9 secure the moving direction of mobile bar 8 so that mobile bar 8 drives clamping plate along the direction perpendicular to side
It is moved, when four rectification plates 10 are respectively further from four sides of plate 4, sheet glass can be placed on plate 4 or be incited somebody to action
Sheet glass is removed from plate 4, when four rectification plates 10 are close mobile to four sides of plate 4 respectively, four rectification plates
10 are resisted against four sides of sheet glass simultaneously, the position of sheet glass are adjusted, so that sheet glass is fixed on the top of plate 4, and glass
Piece is located at the surface of plate 4, carries out printing operation convenient for the point sample mechanism of top.
As shown in Figs. 1-2, the point sample mechanism includes first motor 11, shaft, the sliding block 13, four of circular frame 12, four
Sunpender 14 and four point sample components and four connection components, the first motor 11 are fixed on the lower section of top plate 2, first electricity
Machine 11 is electrically connected with PLC, and the bottom end of the first motor 11 and shaft is sequentially connected, and four connection components, which are circumferentially uniformly distributed, to be turned
The periphery of the bottom end of axis, the connection component are located at the inside of circular frame 12, the both ends of the connection component respectively with two cunnings
Block 13 connects, and the circular frame 12 is parallel with top plate 2, and the quadrangle distribution of the circular frame 12 is fixed on top by four sunpenders 14
The lower section of plate 2, four sliding blocks 13 are respectively sleeved on four sides of circular frame 12, and the point sample component and sliding block 13 1 are a pair of
It answers, the point sample component is located at the lower section of sliding block 13, and the point sample component is connect with hopper 5.
In order to improve the point sample efficiency to sheet glass, PLC controls the point of 13 lower section of sliding block on 12 4 sides of circular frame
Sample component carries out printing operation to four sides of sheet glass, and after the completion of single point sample, PLC controls first motor 11 and starts, and drives
Shaft rotation, shaft is acted on sliding block 13 by connection component, so that four sides of four sliding blocks 13 respectively along circular frame 12
It is moved, changes the position of point sample component, then controlled point sample component again and carry out printing operation, so operate, realize and set
It is standby that point sample is carried out to four sides of sheet glass simultaneously, it improves work efficiency.
As shown in figure 4, the driving component includes the second motor 15, turntable 16 and four drive rods 17, second electricity
Machine 15 is fixed on the lower section of plate 4, and second motor 15 is electrically connected with PLC, and second motor 15 connects with the transmission of turntable 16
It connects, the circumferential periphery for being evenly distributed on turntable 16 of four drive rods 17, the drive rod 17 is corresponded with corrective component, described
One end of drive rod 17 and turntable 16 are hinged, and the other end and mobile bar 8 of the drive rod 17 are hinged.
PLC controls the starting of the second motor 15, and turntable 16 is driven to turn an angle, and turntable 16 drives 17 turns of drive rod
Dynamic, drive rod 17 acts in mobile bar 8, and mobile bar 8 is driven to be translated under the position-limiting action of fixed ring 9.
Preferably, the stable rotation in order to guarantee turntable 16, the periphery of the turntable 16 is equipped with several sliding shoes 18, institute
The lower section for stating plate 4 is equipped with annular groove 19, and the sliding shoe 18 matches with annular groove 19, the sliding shoe 18 and annular groove 19
It is slidably connected.Using the annular groove 19 for being fixed on 4 lower section of plate, the rotary motion trace of sliding shoe 18 is secured, so that turntable 16 is protected
Keep steady fixed rotation.
Preferably, the annular groove 19 is dovetail groove in order to further ensure the stable rotation of turntable 16.Due to annular
Slot 19 is dovetail groove, so that sliding shoe 18 can not be detached from annular groove 19, sliding shoe 18 carries out stable sliding in annular groove 19,
Further ensure the stable rotation of turntable 16.
As shown in Fig. 2, the connection component includes connecting rod 20, connecting tube 21 and two connection units, the connecting rod
20 one end is fixedly connected with shaft, is fixedly connected at the other end of the connecting rod 20 and the center of connecting tube 21, two companies
Order member is located at the both ends of connecting tube 21, and the connection unit includes slide plate 22 and slide bar 23, the periphery of the slide plate 22
Be tightly connected with the inner wall of connecting tube 21, the slide plate 22 is fixedly connected with one end of slide bar 23, the slide bar 23 it is another
End is hinged with sliding block 13, and through-hole is equipped at the center of the side of the separate connecting rod 20 of the connecting tube 21.
Shaft rotation drives connecting tube 21 to be rotated, changes the direction of connecting tube 21 by connecting rod 20, so that connection
The sliding block 13 that the connection unit at 21 both ends of pipe is connected is slided along the side of circular frame 12, between two adjacent sliders 13
Distance change, at this time in connection unit, slide plate 22 can be attached to connecting tube 21 inner wall sliding, facilitate slide bar 23 relative to
Connecting tube 21 is moved, so that connecting tube 21 and the entire length of two connecting rods 20 change, is convenient for two sliding blocks 13
When sliding on circular frame 12, distance between the two changes.
Preferably, the connection unit further includes stop collar 24, the limit for the moving direction of fixed slip bar 23
Ring 24 is fixed on the inner wall of connecting tube 21, and the stop collar 24 is set on slide bar 23.Slide bar is secured using stop collar 24
23 moving direction so that slide bar 23 can be moved along the axis of stop collar 24, while avoiding slide plate 22 and being disconnected
Pipe 21 limits the sliding position of connecting rod 20.
Preferably, in order to avoid, into ash, the connecting tube 21 is interior to be equipped with strainer 25, and the strainer 25 covers in connecting tube 21
It is located at through hole.The air entered inside connecting tube 21 using 25 Duis of strainer is filtered, and avoids influencing in connecting tube 21 into ash
The sliding of slide plate 22.
As shown in figure 3, the point sample component includes dropper 26, microcap 27, communicating pipe 28, sealing plate 29, lifting unit
With two vertical bars 30, two vertical bars 30 are located at the two sides of microcap 27, and the microcap 27 is fixed on cunning by vertical bar 30
The lower section of block 13, the lifting unit are located at the lower section of sliding block 13, and the lifting unit and sealing plate 29 are sequentially connected, described close
The periphery of sealing plate 29 and the inner wall of microcap 27 are tightly connected, and the dropper 26 is fixed on the bottom end of microcap 27, the dropper
Valve is equipped in 26, the valve is electrically connected with PLC, and the bottom end of the dropper 26 is connected by the bottom of communicating pipe 28 and hopper 5
It is logical.
In point sample component, microcap 27 is fixed on to the lower section of sliding block 13 by two vertical bars 30, convenient for microcap 27 with
The movement of sliding block 13 moved, facilitate the sample liquid in hopper 5 to enter in expects pipe using communicating pipe 28, lifting unit is controllable
Sealing plate 29 carries out lifting moving in microcap 27, and PLC controls lifting assembly starting, and valve is closed, and lifting assembly drives close
Sealing plate 29 moves up, and the sample liquid in hopper 5 can be pumped into microcap 27, then PLC control valve is opened, lifting unit band
Dynamic sealing plate 29 moves down, and the sample liquid in microcap 27 can be dripped on the glass sheet by dropper 26, realize printing operation.
Preferably, in order to control the lifting moving of sealing plate 29, the lifting unit includes third motor 31, screw rod 32
With casing 33, the third motor 31 is fixed on the lower section of sliding block 13, and the third motor 31 is electrically connected with PLC, the third
The top of motor 31 and screw rod 32 is sequentially connected, and the bottom end of the screw rod 32 is arranged in casing 33, and described sleeve pipe 33 is fixed on
The lower section of sealing plate 29, described sleeve pipe 33 be equipped with the junction of screw rod 32 and the matched screw thread of screw rod 32.PLC controls third
Motor 31 starts, and screw rod 32 is driven to be rotated, and screw rod 32 passes through threaded function on casing 33, so that casing 33 is along screw rod
32 axis is moved, and then sealing plate 29 is driven to carry out lifting moving.
Preferably, the feature strong using DC servo motor driving force, in order to guarantee the driving force of first motor 11, institute
Stating first motor 11 is DC servo motor.
Sheet glass is placed on plate 4 by user when using the spot sample device, then operates equipment by controller 3
Operation, is acted on the corrective component of surrounding by driving assembly, drives rectification plate 10 simultaneously to the four of plate 4 by mobile bar 8
Side, so that sheet glass is fixed on the surface of plate 4, accurately carries out point sample close to movement convenient for point sample mechanism on the glass sheet,
After extracting the sample liquid progress single point sample in hopper 5 using point sample component, first motor 11 drives shaft rotation, passes through connection group
Part drives four sliding blocks 13 to be moved respectively along four strip side plates 6 of circular frame 12, is then carried out a little by point sample component again
Sample operation, so realizes automatical and efficient point sample, improves the practicability of equipment.
Compared with prior art, straightening machine should be passed through based on the spot sample device for aflatoxins detection of thin layer chromatography
Sheet glass is fixed on the surface of plate 4 by structure, and convenient for point sample mechanism, accurately surface carries out point sample on the glass sheet, not only such as
Four sides above sheet glass are carried out automatic point sample simultaneously using point sample mechanism, improve point sample efficiency, set to improve by this
Standby practicability.
Taking the above-mentioned ideal embodiment according to the present invention as inspiration, through the above description, relevant staff is complete
Various changes and amendments can be carried out without departing from the scope of the technological thought of the present invention' entirely.The technology of this invention
Property range is not limited to the contents of the specification, it is necessary to which the technical scope thereof is determined according to the scope of the claim.
Claims (10)
1. it is a kind of based on thin layer chromatography for aflatoxins detection spot sample device, which is characterized in that including pedestal (1),
Top plate (2), controller (3), plate (4), correction mechanism, point sample mechanism, hopper (5), two side plates (6) and four stabilizer blades (7),
The side plate (6) is fixed on the top of pedestal (1), and the top plate (2) is erected on two side plates (6), and the controller (3) is solid
It being scheduled on one of side plate (6), PLC is equipped in the controller (3), the hopper (5) is fixed on the top of top plate (2),
The four corners of the plate (4) pass through the top that four stabilizer blades (7) are fixed on pedestal (1) respectively, and the correction mechanism is located at flat
The lower section of plate (4), the point sample mechanism are located at the lower section of top plate (2)；
The correction mechanism includes driving assembly and four corrective components, and four corrective components are located at four sides of plate (4),
The driving component is located at the underface of plate (4), and the corrective component includes mobile bar (8), fixed ring (9) and rectification plate
(10), one end of the driving component and mobile bar (8) is sequentially connected, and the rectification plate (10) is fixed on the another of mobile bar (8)
The top of one end, the fixed ring (9) are fixed on the lower section of plate (4), and the fixed ring (9) is set on mobile bar (8)；
The point sample mechanism include first motor (11), shaft, circular frame (12), four sliding blocks (13), four sunpenders (14) and
Four point sample components and four connection components, the first motor (11) are fixed on the lower section of top plate (2), the first motor
(11) it is electrically connected with PLC, the bottom end of the first motor (11) and shaft is sequentially connected, and four connection components are circumferentially uniformly distributed
The periphery of the bottom end of shaft, the connection component are located at the inside of circular frame (12), and the both ends of the connection component are respectively with two
A sliding block (13) connection, the circular frame (12) is parallel with top plate (2), and the quadrangle distribution of the circular frame (12) is hung by four
Bar (14) is fixed on the lower section of top plate (2), and four sliding blocks (13) are respectively sleeved on four sides of circular frame (12), the point
Sample component and sliding block (13) correspond, and the point sample component is located at the lower section of sliding block (13), the point sample component and hopper (5)
Connection.
2. the spot sample device for aflatoxins detection based on thin layer chromatography as described in claim 1, which is characterized in that
The driving component includes that the second motor (15), turntable (16) and four drive rods (17), second motor (15) are fixed on
The lower section of plate (4), second motor (15) are electrically connected with PLC, and second motor (15) and turntable (16) are sequentially connected,
Four drive rods (17) are circumferentially evenly distributed on the periphery of turntable (16), and the drive rod (17) and corrective component correspond,
Hingedly, the other end of the drive rod (17) and mobile bar (8) are hinged for one end and turntable (16) of the drive rod (17).
3. the spot sample device for aflatoxins detection based on thin layer chromatography as claimed in claim 2, which is characterized in that
The periphery of the turntable (16) is equipped with several sliding shoes (18), and the lower section of the plate (4) is equipped with annular groove (19), the sliding
Block (18) matches with annular groove (19), and the sliding shoe (18) is slidably connected with annular groove (19).
4. the spot sample device for aflatoxins detection based on thin layer chromatography as claimed in claim 3, which is characterized in that
The annular groove (19) is dovetail groove.
5. the spot sample device for aflatoxins detection based on thin layer chromatography as described in claim 1, which is characterized in that
The connection component includes connecting rod (20), connecting tube (21) and two connection units, one end of the connecting rod (20) with turn
Axis is fixedly connected, and is fixedly connected at the other end of the connecting rod (20) and the center of connecting tube (21), two connection units point
Not Wei Yu connecting tube (21) both ends, the connection unit includes slide plate (22) and slide bar (23), the periphery of the slide plate (22)
It is tightly connected with the inner wall of connecting tube (21), the slide plate (22) is fixedly connected with one end of slide bar (23), the slide bar
(23) the other end and sliding block (13) hingedly, at the center of the side of the separate connecting rod (20) of the connecting tube (21) is equipped with logical
Hole.
6. the spot sample device for aflatoxins detection based on thin layer chromatography as claimed in claim 5, which is characterized in that
The connection unit further includes stop collar (24), and the stop collar (24) is fixed on the inner wall of connecting tube (21), the limit
Ring (24) is set on slide bar (23).
7. the spot sample device for aflatoxins detection based on thin layer chromatography as claimed in claim 5, which is characterized in that
Strainer (25) are equipped in the connecting tube (21), strainer (25) lid is located at through hole.
8. the spot sample device for aflatoxins detection based on thin layer chromatography as described in claim 1, which is characterized in that
The point sample component includes dropper (26), microcap (27), communicating pipe (28), sealing plate (29), lifting unit and two vertical bars
(30), two vertical bars (30) are located at the two sides of microcap (27), and the microcap (27) is fixed on cunning by vertical bar (30)
The lower section of block (13), the lifting unit are located at the lower section of sliding block (13), and the lifting unit and sealing plate (29) are sequentially connected,
The periphery of the sealing plate (29) and the inner wall of microcap (27) are tightly connected, and the dropper (26) is fixed on microcap (27)
Bottom end, the dropper (26) is interior to be equipped with valve, and the valve is electrically connected with PLC, and the bottom end of the dropper (26) passes through communicating pipe
(28) it is connected to the bottom of hopper (5).
9. the spot sample device for aflatoxins detection based on thin layer chromatography as claimed in claim 8, which is characterized in that
The lifting unit includes third motor (31), screw rod (32) and casing (33), and the third motor (31) is fixed on sliding block
(13) lower section, the third motor (31) are electrically connected with PLC, and the top of the third motor (31) and screw rod (32), which is driven, to be connected
It connects, in casing (33), described sleeve pipe (33) is fixed on the lower section of sealing plate (29), described for the bottom end setting of the screw rod (32)
Casing (33) be equipped with the junction of screw rod (32) and screw rod (32) matched screw thread.
10. the spot sample device for aflatoxins detection based on thin layer chromatography, feature exist as described in claim 1
In the first motor (11) is DC servo motor.
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| CN201910408453.0A CN110082473A (en) | 2019-05-16 | 2019-05-16 | A kind of spot sample device for aflatoxins detection based on thin layer chromatography |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910408453.0A CN110082473A (en) | 2019-05-16 | 2019-05-16 | A kind of spot sample device for aflatoxins detection based on thin layer chromatography |
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| CN110082473A true CN110082473A (en) | 2019-08-02 |
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| CN201910408453.0A Withdrawn CN110082473A (en) | 2019-05-16 | 2019-05-16 | A kind of spot sample device for aflatoxins detection based on thin layer chromatography |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115097059A (en) * | 2022-06-15 | 2022-09-23 | 范洋 | Traditional chinese medicine clinical laboratory is with traditional chinese medicine thin layer chromatography verifying attachment |
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