CN110078560A - A kind of preparation method of nitrogen-phosphorus synergist - Google Patents
A kind of preparation method of nitrogen-phosphorus synergist Download PDFInfo
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- CN110078560A CN110078560A CN201910416136.3A CN201910416136A CN110078560A CN 110078560 A CN110078560 A CN 110078560A CN 201910416136 A CN201910416136 A CN 201910416136A CN 110078560 A CN110078560 A CN 110078560A
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- parts
- nitrogen
- mucus
- loach
- phosphorus
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- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 241000252185 Cobitidae Species 0.000 claims abstract description 42
- 210000003097 mucus Anatomy 0.000 claims abstract description 41
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 22
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 17
- 239000011591 potassium Substances 0.000 claims abstract description 17
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 17
- 229920000805 Polyaspartic acid Polymers 0.000 claims abstract description 15
- 108010064470 polyaspartate Proteins 0.000 claims abstract description 15
- 241000192656 Nostoc Species 0.000 claims abstract description 13
- 239000004220 glutamic acid Substances 0.000 claims abstract description 12
- VQTVFIMEENGCJA-UHFFFAOYSA-N 3,4-dimethyl-1H-pyrazole Chemical compound CC=1C=NNC=1C VQTVFIMEENGCJA-UHFFFAOYSA-N 0.000 claims abstract description 11
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims abstract description 11
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 claims abstract description 11
- -1 hexamethylene phosphoric triamide Chemical compound 0.000 claims abstract description 9
- 235000013311 vegetables Nutrition 0.000 claims abstract description 6
- 230000000694 effects Effects 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 102000000422 Matrix Metalloproteinase 3 Human genes 0.000 claims description 10
- 108010016160 Matrix Metalloproteinase 3 Proteins 0.000 claims description 10
- 235000013399 edible fruits Nutrition 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 5
- 241000194108 Bacillus licheniformis Species 0.000 claims description 3
- 241000194107 Bacillus megaterium Species 0.000 claims description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 3
- 108090000787 Subtilisin Proteins 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 239000000084 colloidal system Substances 0.000 claims description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 3
- 238000001976 enzyme digestion Methods 0.000 claims description 3
- 125000004836 hexamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 claims description 3
- 238000003760 magnetic stirring Methods 0.000 claims description 3
- 238000005360 mashing Methods 0.000 claims description 3
- 239000002002 slurry Substances 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- 238000012271 agricultural production Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 230000007062 hydrolysis Effects 0.000 claims 2
- 238000006460 hydrolysis reaction Methods 0.000 claims 2
- 241000193755 Bacillus cereus Species 0.000 claims 1
- 235000014459 Sorbus Nutrition 0.000 claims 1
- 241001092391 Sorbus Species 0.000 claims 1
- 108010089934 carbohydrase Proteins 0.000 claims 1
- 210000004907 gland Anatomy 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 40
- 239000002689 soil Substances 0.000 abstract description 26
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 15
- 239000011574 phosphorus Substances 0.000 abstract description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 11
- 201000010099 disease Diseases 0.000 abstract description 10
- 238000002474 experimental method Methods 0.000 abstract description 2
- 210000003608 fece Anatomy 0.000 abstract 1
- 239000010871 livestock manure Substances 0.000 abstract 1
- 239000003337 fertilizer Substances 0.000 description 31
- 235000015097 nutrients Nutrition 0.000 description 19
- 238000012360 testing method Methods 0.000 description 17
- 239000000126 substance Substances 0.000 description 15
- 150000001875 compounds Chemical class 0.000 description 8
- 229940088598 enzyme Drugs 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 241000209094 Oryza Species 0.000 description 6
- 235000007164 Oryza sativa Nutrition 0.000 description 6
- 240000006365 Vitis vinifera Species 0.000 description 6
- 235000014787 Vitis vinifera Nutrition 0.000 description 6
- 229940024606 amino acid Drugs 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 235000021049 nutrient content Nutrition 0.000 description 6
- 230000008635 plant growth Effects 0.000 description 6
- 235000009566 rice Nutrition 0.000 description 6
- 238000012795 verification Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 235000009754 Vitis X bourquina Nutrition 0.000 description 5
- 235000012333 Vitis X labruscana Nutrition 0.000 description 5
- 230000009102 absorption Effects 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 239000000618 nitrogen fertilizer Substances 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002686 phosphate fertilizer Substances 0.000 description 4
- 235000013824 polyphenols Nutrition 0.000 description 4
- 230000002195 synergetic effect Effects 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 229910002651 NO3 Inorganic materials 0.000 description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 3
- 230000002708 enhancing effect Effects 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000003375 plant hormone Substances 0.000 description 3
- 150000008442 polyphenolic compounds Chemical class 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000012747 synergistic agent Substances 0.000 description 3
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 239000002509 fulvic acid Substances 0.000 description 2
- 229940095100 fulvic acid Drugs 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- SDXAWLJRERMRKF-UHFFFAOYSA-N 3,5-dimethyl-1h-pyrazole Chemical compound CC=1C=C(C)NN=1 SDXAWLJRERMRKF-UHFFFAOYSA-N 0.000 description 1
- 108010011619 6-Phytase Proteins 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 102000030523 Catechol oxidase Human genes 0.000 description 1
- 108010031396 Catechol oxidase Proteins 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000219991 Lythraceae Species 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 241000243785 Meloidogyne javanica Species 0.000 description 1
- 241000192543 Nostocaceae Species 0.000 description 1
- 240000005928 Pogostemon auricularius Species 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- DHKHZGZAXCWQTA-UHFFFAOYSA-N [N].[K] Chemical compound [N].[K] DHKHZGZAXCWQTA-UHFFFAOYSA-N 0.000 description 1
- 239000000370 acceptor Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 229930014669 anthocyanidin Natural products 0.000 description 1
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 1
- 235000008758 anthocyanidins Nutrition 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 210000001990 heterocyst Anatomy 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 230000000622 irritating effect Effects 0.000 description 1
- 239000008297 liquid dosage form Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 125000000962 organic group Chemical group 0.000 description 1
- 238000012946 outsourcing Methods 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 125000004151 quinonyl group Chemical group 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B15/00—Organic phosphatic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
The present invention provides a kind of nitrogen-phosphorus synergist and its preparation method and application containing loach and its mucus hydrolyzate, nitrogen-phosphorus synergist of the present invention containing loach and its mucus hydrolyzate, according to parts by weight, including loach and its 30-45 parts of mucus hydrolyzate, 30-60 parts of Black Box Tracing pomace, 5-15 parts of nostoc proteoglycan, 15-30 parts of potassium fulvate, 15-40 parts of poly-aspartic-acid, 1-5 parts of hexamethylene phosphoric triamide, 0.5-5 parts of 3,4 dimethyl pyrazole, 1-6 parts of a- Nafusaku, 3-9 parts of study on brassinolide, 2-5 parts of poly-gamma-glutamic acid.Show the use that facilities vegetable yield can be improved using nitrogen-phosphorus synergist of the present invention, reduce nitrogenous-phosphatic manure by planting experiment, improve the content of rapid available phosphorus and available nitrogen in soil, soil quality can also be improved, inhibit soil-borne disease.
Description
Technical field
The invention belongs to agricultural technology fields, and in particular to a kind of nitrogen-phosphorus synergist and preparation method and application.
Background technique
In order to pursue the high yield of the crops such as grain, vegetables, peasant often largely applies the fertilizer such as nitrogen, phosphorus, therefore farmland
Soil Nitrogen, phosphorus input amount are far longer than crop demand.If things go on like this, the nutrient content in soil is caused constantly to be accumulated, crop
It is serious to the reduction of nitrogen P assimilation and utilization rate, nitrogen and phosphorus loss, not only nutrient is caused to waste, returns soil, underground water and agricultural ecological
Environment etc. brings serious pollution risk.In order to reduce nitrogen and phosphorus pollution, the efficient utilization of nutrient is realized, except reducing nitrogen from source
Outside phosphorus precipitation, it is often more important that improve nitrogen and phosphorus nutrient utilization rate.The prior art is generally from enhancing nitrogen phosphorus activity, improvement soil
Environment promotes being absorbed and utilized for nitrogen and phosphorus nutrient.
Biochemical in loach and its mucus body fluid plays an important role, loach that loach and its mucus are hydrolyzed to form and
Containing a large amount of beneficial to improvement soil, the active material improved the ecological environment, promote plant growth etc. in its mucus hydrolyzate, such as
Content polyphenol, plant hormone, amino acid etc., soil-borne disease insect pest can be inhibited, promote plant growth, improve soil quality etc..Mud
The application of loach and its mucus hydrolyzate at present in agricultural production is embodied in and promotes plant growth, volume increase, disease-resistant, raising crop
Quality, and improve soil environment etc..There has been no nitrogen and phosphorus nutrient utilization rate is improved using loach and its mucus hydrolyzate
Correlation grinds the report that makes internal disorder or usurp.
Black Box Tracing fruit has outstanding health-care effect.Fruit and its extract are to the heart and brain blood such as heart disease, hypertension
Pipe disease has special curative effect, is widely used in medicine and functional food industry in European and American areas.Anthocyanidin, flavones in fruit
(fresh fruit content is up to 0.25%-0.35%), polyphenol are that content is highest in known plants.Fruit also contains multivitamin and mine
The substances such as prime element, pomace nitrogen P elements rich in play an important role to organic fertilizer nitrogen phosphorus is promoted.
Nostoc (latin name: Pogostemon auricularius (L.) Kassk;Scientific name: intend spherical nostoc) it is commonly called as beauty
Rice, day rosetangle, loosestrife, water agaric, Tian Muer.Nostoc Cyanophyceae, nostocaceae.Frond in colloidal substance, it is spherical or other
Irregular shape, blue-green or yellowish-brown.CellSpherical shape is linked to be beads shape group, outsourcing colloid by most cells.Heterocyst position
In the iuntercellular of filament, young filament is frequently located in top.BreedingWhen majority generate akinete, often to concatenate into chain.It is wet
When in green, be in grey black after dry.Between attached submersed sandstone or on dark and damp soil.It is distributed all over China, with
Sichuan produces most famous.It is edible.With nitrogen fixing capacity.Containing a variety of amino acid needed by human andPolysaccharideIsoreactivity object
Matter, proteoglycan play a significant role to nitrogen phosphorus fertilizer power is promoted.
Potassium fulvate: its action principle mainly includes the following aspects: (1) improving soil, potassium fulvate and other organic gels
Body forms colloid substance in the soil, and soil particle cementation is got up, and increases water-stable granular structure;Potassium fulvate contains a large amount of
Active group is more, increases baseexchangecapacity, and absorption and the large number of deleterious cations of detention reduce soil salinity
And pH value, so as to improve the ecological environment of crop.(2) fertilizer efficiency is improved, by the acting activating many mineral elements of exchange adsorption,
Improve fertilizer efficiency.The effect of nitrogenous fertilizer synergy is shown: reducing nitrogen volatilization loss, synergistic effect is highly significant to urea, can
So that the fertilizer efficiency of urea extends, promote the absorption of nitrogen, improves utilization rate of nitrogen fertilizer.The synergistic effect of phosphate fertilizer is shown: reducing soil
Earth plays the role of phosphorus decomposing to quick-acting phosphorus fixations.Make insoluble phosphate-solubilizing available phosphorus in soil, promotes crops to phosphorus
It absorbs.(3) promote crop growth, functional group, the life as physiological activator, to crop containing there are many in potassium fulvate
Long development and body physiological are metabolized irritating effect, the active group of fulvic acid (including carboxyl, phenolic hydroxyl group, quinonyl, hydroxyl and
Certain groups containing P, 0, N, S) be typically all electronq donor, it is easy to many electron acceptors (polyvalent metal ion,
Organic group or ion) such as the substance composition complex in loach and its mucus hydrolyzate, referred to as complex compound or chelate.
To be beneficial to prevent nitrogen volatilization, be lost, prevent P, etc. by soil fixing, improve their validity and long-term effect, very
It is closely integrated into chemical bond to fulvic acid and these nutrients, is absorbed and is utilized together by plant, so the utilization of nutrient can be improved
Rate.
Poly-aspartic-acid: poly-aspartic-acid is negatively charged, and the poly-aspartic-acid of macromolecular can have negative ions centainly
Suction-operated, it is especially preferable to the adsorption effect of nitrogen potassium.It can promote agriculture in conjunction with a variety of nutrient elements as fertilizer synergist
Nutrient is absorbed and utilized in crop, is plant nutrient sorbefacient.
Summary of the invention
The present invention in view of the above shortcomings of the prior art, the characteristics of according to loach and its mucus hydrolyzate, provides a kind of nitrogen
Phosphorus synergist and its preparation method and application.For achieving the above object, The technical solution adopted by the invention is as follows: by weight
Number meter, including loach and its 30-45 parts of mucus hydrolyzate, 30-60 parts of Black Box Tracing pomace, nostoc proteoglycan 5-
15 parts, 15-30 parts of potassium fulvate, 15-40 parts of poly-aspartic-acid, 1-5 parts of hexamethylene phosphoric triamide, 3,4 dimethyl pyrazole 0.5-5
Part, 1-6 parts of a- Nafusaku, 3-9 parts of study on brassinolide, 2-5 parts of poly-gamma-glutamic acid.
Specifically, the nitrogen-phosphorus synergist is liquid dosage form, pulvis or granule.
Including loach and its 32 parts of mucus hydrolyzate, 45 parts of Black Box Tracing pomace, Ge Xian specifically, according to parts by weight,
10 parts of rice gluten polysaccharide, 15 parts of potassium fulvate, 20 parts of poly-aspartic-acid, 1 part of hexamethylene phosphoric triamide, 3,4 dimethyl pyrazoles 1
Part, 3 parts of a- Nafusaku, 3 parts of study on brassinolide, 5 parts of poly-gamma-glutamic acid.A kind of preparation method of nitrogen-phosphorus synergist, it is special
Sign is that steps are as follows:
(1) preparation of loach and its mucus hydrolyzate: fresh loach and its mucus, Black Box Tracing pomace smashed to pieces obtain it is even
Slurry is added water and adjusts pH to 6-8, and complex enzyme is added, and carries out digesting up to hydrolyzate under the conditions of insulated and stirred
(2) the preparation of nitrogen-phosphorus synergist, by hydrolyzate, nostoc proteoglycan, potassium fulvate, poly-aspartic-acid, hexamethylene phosphinylidyne
Triamine, 3,4 dimethyl pyrazoles, a- Nafusaku, study on brassinolide, poly-gamma-glutamic acid and/or water are stirred 20-
30min is to get nitrogen-phosphorus synergist.
Specifically, the complex enzyme is bacillus megaterium stromelysin 1, bacillusmusilaginosiengineering stromelysin 1, glue
Matter bacillus protein polysaccharase, subtilisin polysaccharase and bacillus licheniformis stromelysin 1 are according to 1-2:
The mixing of 0.5-3:1-1.5:1.5-4:2-6 ratio.
Specifically, the step (1) takes fresh loach and its mucus 100-200g, with sterile water wash 3-5 times, by clean mud
Loach and its mucus are placed in high-speed tissue mashing machine, are smashed 25-30min to pieces with the revolving speed of 4000-5000r/min, are homogenized,
Homogenate is added in beaker with water 1:2-1:4 in mass ratio, adjusts pH to 6-8 with the dilute hydrochloric acid that concentration is 0.02mol/L,
300-500mg complex enzyme is added, after being stirred 3-5min, beaker is moved into heat-collecting magnetic stirring device, adjusts stirring
Device temperature is to 40-45 DEG C, speed of agitator to 200-230r/min, enzyme digestion reaction 4-5h, and enzymatic hydrolysis terminates, by enzymolysis liquid be put into from
In scheming, 20-25min is centrifuged with the revolving speed of 3500-4500r/min, takes upper liquid, i.e. loach and its mucus hydrolyzate.
Specifically, the loach of step (1) preparation and its mucus hydrolyzate are concentrated and dried is prepared at pulvis and other components mixing
Pulvis, or be pressed further by and be prepared into particle.
Specifically, application of the nitrogen-phosphorus synergist in facilities vegetable.
Specifically, synergist amount of application in facilities vegetable is 30-40kg/ mus.
Loach of the present invention and its mucus hydrolyzate: contain polyphenol, plant hormone, amino acid and the unknown various active of other compositions
Substance can not only inhibit soil-borne disease insect pest, promote plant growth, improve soil quality, it is often more important that can also improve fertilizer
Expect utilization rate.
It is even more amino rich in the loach and its mucus hydrolyzate of acquisition that the present invention, which digest by using complex enzyme,
Sour and a small amount of small peptide, wherein amino acid accounts for 85%, and small peptide and small molecular protein polysaccharide matter account for 14%-15%.Amino acid is promoting
Plant growth, improve crop yield, enhancing nitrogen phosphorus nutrient on remarkably promote effect.
Antibacterial substance: there are a variety of antimicrobial components in loach and its slime body can not only provide such as antibacterial peptide material for plant
Quickly and effectively nutrient, moreover it is possible to which the growth for inhibiting germ plays a significant role in terms of preventing soil-borne disease insect pest, and cover crop is exempted from
By virus infraction, guarantees that crop normally produces, improve crop yield, promote a large amount of absorptions to nitrogen and phosphorus nutrient.
The synergistic effect of each component is also embodied in the various substances pair of loach and its mucus hydrolyzate and addition in inventive formulation
Promote that there is effective, significant coordination with the synthesis effect in terms of nitrogen and phosphorus nutrient utilization rate.Object in loach and its mucus hydrolyzate
Matter, including amino acid, antibacterial substance, plant hormone etc. can improve crop immunocompetence, reduce pest and disease damage, and can promote to make
Object growth, provides nutrient for plant growth, and there are also some substances that do not recognize at present to play positive facilitation.Addition
The substances such as phytase, poly-aspartic-acid have the active effect of obligate promotion nitrogen phosphorus, loach and its mucus hydrolyzate and addition
Various substances between exist mutually cooperate with, promote effect, improve crop disease resistance while, crop can be promoted again
Growth promotes the absorption to nitrogen P elements, therefore, more significant to the synergistic effect of nitrogen phosphorus.
Compared with prior art, the present invention has the following advantages:
(1) improves N,P use efficiency, reduces nitrogen and phosphorous fertilizer use.It can be by utilization rate of nitrogen fertilizer using nitrogen-phosphorus synergist of the present invention
30%-35% is improved, phosphate fertilizer utilization efficiency improves 25%-35%.The average nitrogen and phosphorous fertilizer that can save per acre uses 40kg or more.
(2) crop yield is improved.After practical planting experiment shows to apply nitrogen-phosphorus synergist of the present invention, nitrogen phosphorus is being reduced per acre
Under the premise of fertilizer application 40kg, crop yield can also be improved, averagely increases production 16% or more.
(3) improve soil quality.Enzyme Activities in Soils can be significantly improved using nitrogen-phosphorus synergist of the present invention, polyphenol oxidase and
Activity of acid phosphatase improves 8.78%-20.4%, and the effective manganese content of microelement improves 30.0%, and soil pH value is without significant
Variation, soil activation enhancing, available nutrient content improve.
(4) inhibits soil-borne disease.Graw mold of tomato disease incidence is set to reduce by 9% using nitrogen-phosphorus synergist of the present invention, root-knot nematode sense
Strain rate of catching an illness reduces, and controls in 2%-5%.
Specific embodiment
Every part is 1 kilogram below.
Embodiment 1
Fertilizer synergistic agent prescription 1 is as follows:
Loach and its 30 parts of mucus hydrolyzate, 30 parts of Black Box Tracing pomace, 5 parts of nostoc proteoglycan, potassium fulvate 15
In part, 15 parts of poly-aspartic-acid, 1 part of hexamethylene phosphoric triamide, 0.5 part of 3,4 dimethyl pyrazole, 1 part of a- Nafusaku, rape are plain
3- parts of ester, 2 parts of poly-gamma-glutamic acid.
Embodiment 2
Fertilizer synergistic agent prescription 2 is as follows:
40 parts of loach and its mucus hydrolyzate, 10 parts of nostoc proteoglycan, 20 parts of potassium fulvate, gather 35 parts of Black Box Tracing pomace
20 parts of asparatate, 2 parts of hexamethylene phosphoric triamide, 1 part of 3,4 dimethyl pyrazole, 2 parts of a- Nafusaku, 5 parts of study on brassinolide,
3 parts of poly-gamma-glutamic acid.
3 fertilizer synergistic agent prescription 3 of embodiment is as follows:
Loach and its 45 parts of mucus hydrolyzate, 30 parts of potassium fulvate, 40 parts of poly-aspartic-acid, 5 parts of hexamethylene phosphoric triamide, 3,4
5 parts of dimethyl pyrazole, 6 parts of a- Nafusaku, 9 parts of study on brassinolide, 5 parts of poly-gamma-glutamic acid.
The production method of synergist in embodiment 1-3, steps are as follows:
(1) preparation of loach and its mucus hydrolyzate: fresh loach and its mucus, Black Box Tracing pomace smashed to pieces obtain it is even
Slurry is added water and adjusts pH to 6-8, and complex enzyme is added, and carries out digesting up to hydrolyzate under the conditions of insulated and stirred;
(2) the preparation of nitrogen-phosphorus synergist, by hydrolyzate, nostoc proteoglycan, potassium fulvate, poly-aspartic-acid, hexamethylene phosphinylidyne
Triamine, 3,4 dimethyl pyrazoles, a- Nafusaku, study on brassinolide, poly-gamma-glutamic acid and/or water are stirred 20-
30min is to get nitrogen-phosphorus synergist.
Specifically, the complex enzyme is bacillus megaterium stromelysin 1, bacillusmusilaginosiengineering stromelysin 1, glue
Matter bacillus protein polysaccharase, subtilisin polysaccharase and bacillus licheniformis stromelysin 1 are according to 1:0.5:
The mixing of 1.5:4:6 ratio.
Specifically, the step (1) takes fresh loach and its mucus 100-200g, with sterile water wash 3-5 times, by clean mud
Loach and its mucus are placed in high-speed tissue mashing machine, are smashed 25-30min to pieces with the revolving speed of 4000-5000r/min, are homogenized,
Homogenate is added in beaker with water 1:2-1:4 in mass ratio, adjusts pH to 6-8 with the dilute hydrochloric acid that concentration is 0.02mol/L, then
300-500mg complex enzyme is added, after being stirred 3-5min, beaker is moved into heat-collecting magnetic stirring device, adjusts blender
Temperature is to 40-45 DEG C, speed of agitator to 200-230r/min, enzyme digestion reaction 4-5h, and enzymatic hydrolysis terminates, and enzymolysis liquid is put into centrifugation
In machine, 20-25min is centrifuged with the revolving speed of 3500-4500r/min, takes upper liquid, i.e. loach and its mucus hydrolyzate.
Specifically, the loach of step (1) preparation and its mucus hydrolyzate are concentrated and dried is prepared at pulvis and other components mixing
Pulvis, or be pressed further by and be prepared into particle.
Verification test (one): using effect of the present invention to rice
Verification test: with embodiment 1, test plot be Henan Province Tanghe County county Ma Zhen help, by fertilizer synergist 4kg with it is compound
Fertilizer 35kg is puddled uniformly, is 35kg/ mus by dose and is applied farmland for synergist/compound fertilizer as base fertilizer, the later period is no longer
Top dressing.Nutrient content is N-P-K=28-8-8 in compound fertilizer.
Comparative test: test plot is that Henan Province Tanghe County county Ma Zhen is helped, and is 45kg/ mus using compound fertilizer the bottom of as by dose
Fertile primary application, later period no longer top dressing.Nutrient content is N-P-K=28-8-8 in compound fertilizer.
As a result: application synergist/compound fertilizer plot, when harvest corn, seed golden yellow color, well developed root system, illustrate phosphate fertilizer and
The utilization rate of potash fertilizer is high;With blade, still color is dark green for fringe position, illustrates that the later period does not have that denitrogenation is fertile, in comparative test directly
Compare output increased 5% in the plot for applying compound fertilizer.In the nitrate amount of big bell mouth stage measurement topsoil 20cm, and to photograph
Than the nitrate amount for verifying plot reduces by 87%.
Verification test (two): using effect of the present invention to rice:
Verification test: by taking embodiment 2 as an example, test plot is the paddy field of province, Hubei Province Xiaogan City, by fertilizer synergist 10kg
It puddles uniformly with 15kg ammonium sulfate, is applied in one mu of paddy field as dressing of turnning green stage.
Comparative test: test plot is the paddy field of province, Hubei Province Xiaogan City, and 20kg ammonium sulfate is puddled uniformly, as dressing of turnning green stage
It applies in one mu of paddy field.
As a result: observe after fertilising 20 days: application synergist plot, rice seedlings leaf color is dark green, and tiller number is 2 more than control plot
3 plants/clump, well developed root system, Bai Genduo, it was demonstrated that nitrogenous fertilizer and phosphate fertilizer utilization efficiency are high;When harvest: application synergist plot, rice function
Leaf is still good for green, mass of 1000 kernel increase by 5%, volume increase 9.7%, it was demonstrated that nitrogenous fertilizer and potassium utilization rate are high.
Verification test (three): using effect of the present invention to grape
Verification test: by taking embodiment 3 as an example, in Qixia County, shandong county grape base, amount of application 5kg/ mus is pressed at the florescence, trench digging will
Synergist applies earthing in ditch.Later period no longer applies other chemical fertilizer, only keeps the skin wet.
Comparative test 1: in Qixia County, shandong county grape base, carrying out moisture supplement at the florescence, and the later period also only carries out moisture supplement,
Do not apply chemical fertilizer.
Comparative test 2: in Qixia County, shandong county grape base, the water-soluble fertilizer that nutrient content is 10-30-15 is pressed at the florescence
It 5kg/ mus, fills with water droplet to european grape root, the later period, trickle irrigation applied the water that a nutrient content is 10-10-30 at interval of 20 days
Molten fertilizer, applies 5kg every time, until vintage terminates.
As a result: application synergist plot, compared with 1 plot of comparative test, yield increases by 43%, and fruit is uniform, colours, sugar
It is high;Compared with comparative test 2, yield maintains an equal level, but fruit good evenness, rich in sugar, reduces disease, and few spray is killed three times
Microbial inoculum.
Interpretation of result: in grape base, application water-soluble fertilizer is excessively frequent, and amount of application is excessive, and a large amount of non-available nutrients are accumulated at
It in soil, also results in soil physics, chemistry, biological character and deteriorates, provide condition for harmful microorganism growth.The synergist
The non-available nutrient added up in soil can be activated and carry out for crop absorption utilization.
To sum up, this synergist is for nitrate product in the utilization rate and reduction soil of npk nutrient in comprehensive raising farmland
It is tired to have remarkable effect, under conditions of reducing applying quantity of chemical fertilizer, yield can be increased and improve the quality of fruit.And reducing
The amount of application of fertilizer, that is, save resource and the energy, while reducing the outlet of farmland nutrient, protect environment.In addition, this increasing
Simple production process is imitated, convenient for operation, while each component order by merging of the production method is very scientific, makes synergist obtained
The coordinative role of each component can more be given full play to.
Above only describes various embodiments of the present invention, above description is exemplary, and non-exclusive, and be also not necessarily limited to
Disclosed each embodiment.Without departing from the scope and spirit of illustrated each embodiment, for the art
Those of ordinary skill for many modifications and changes are obvious, be related to those skilled in the art on basis of the invention
On the change made, should all belong to protection scope of the present invention.
Claims (10)
1. a kind of preparation method of nitrogen-phosphorus synergist, according to parts by weight, including it is 30-45 parts of loach and its mucus hydrolyzate, black
30-60 parts of fruit gland rib Sorbus alnifloria pomace, 5-15 parts of nostoc proteoglycan, 15-30 parts of potassium fulvate, poly-aspartic-acid 15-40
It is part, 1-5 parts of hexamethylene phosphoric triamide, 0.5-5 parts of 3,4 dimethyl pyrazole, 1-6 parts of a- Nafusaku, 3-9 parts of study on brassinolide, poly-
2-5 parts of gamma-glutamic acid.
2. nitrogen-phosphorus synergist as claimed in claim 1 or 2, characterized in that according to parts by weight, including loach and its mucus hydrolysis
32 parts of liquid, 45 parts of Black Box Tracing pomace, 10 parts of nostoc proteoglycan, 15 parts of potassium fulvate, 20 parts of poly-aspartic-acid,
1 part of hexamethylene phosphoric triamide, 1 part of 3,4 dimethyl pyrazole, 3 parts of a- Nafusaku, 3 parts of study on brassinolide, 5 parts of poly-gamma-glutamic acid.
3. nitrogen-phosphorus synergist as claimed in claim 1 or 2, characterized in that according to parts by weight, including loach and its mucus hydrolysis
32 parts of liquid, 45 parts of Black Box Tracing pomace, 10 parts of nostoc proteoglycan, 15 parts of potassium fulvate, 20 parts of poly-aspartic-acid,
1 part of hexamethylene phosphoric triamide, 1 part of 3,4 dimethyl pyrazole, 3 parts of a- Nafusaku, 3 parts of study on brassinolide, 5 parts of poly-gamma-glutamic acid.
4. a kind of preparation method of nitrogen-phosphorus synergist according to claim 1, characterized in that steps are as follows:
(1) preparation of loach and its mucus hydrolyzate: fresh loach and its mucus, Black Box Tracing pomace smashed to pieces obtain it is even
Slurry is added water and adjusts pH to 6-8, and complex enzyme is added, and carries out digesting up to hydrolyzate under the conditions of insulated and stirred;
(2) the preparation of nitrogen-phosphorus synergist, by hydrolyzate, nostoc proteoglycan, potassium fulvate, poly-aspartic-acid, hexamethylene phosphinylidyne
Triamine, 3,4 dimethyl pyrazoles, a- Nafusaku, study on brassinolide, poly-gamma-glutamic acid and/or water are stirred 20-
30min is to get nitrogen-phosphorus synergist.
5. the preparation method of nitrogen-phosphorus synergist as claimed in claim 4, characterized in that the complex enzyme is bacillus megaterium
Stromelysin 1, bacillusmusilaginosiengineering stromelysin 1, colloid bacillus cereus stromelysin 1, subtilisin are more
Carbohydrase and bacillus licheniformis stromelysin 1 are mixed according to 1-2:0.5-3:1-1.5:1.5-4:2-6 ratio.
6. the preparation method of nitrogen-phosphorus synergist as described in claim 4 or 5, characterized in that the step (1) takes fresh mud
Clean loach and its mucus are placed in high-speed tissue mashing machine with sterile water wash 3-5 times by loach and its mucus 100-200g
In, 25-30min is smashed to pieces with the revolving speed of 4000-5000r/min, is homogenized, homogenate and water 1:2-1:4 in mass ratio are added
Into beaker, pH to 6-8 is adjusted with the dilute hydrochloric acid that concentration is 0.02mol/L, 300-500mg complex enzyme is added, is stirred
After 3-5min, beaker is moved into heat-collecting magnetic stirring device, adjusts blender temperature to 40-45 DEG C, speed of agitator to 200-
230r/min, enzyme digestion reaction 4-5h, enzymatic hydrolysis terminate, enzymolysis liquid are put into a centrifuge, with the revolving speed of 3500-4500r/min from
Heart 20-25min takes upper liquid, i.e. loach and its mucus hydrolyzate.
7. the preparation method of the nitrogen-phosphorus synergist as described in claim 4-6 is any, characterized in that the mud of step (1) preparation
Loach and its mucus hydrolyzate, which are concentrated and dried, is prepared into pulvis at pulvis and other components mixing, or is pressed further by and is prepared into
Grain.
8. nitrogen-phosphorus synergist a method according to any one of claims 1-3 improves effect of N and P in agricultural production, reduces nitrogen phosphorus face
Application in the pollution of source.
9. application as claimed in claim 8, characterized in that application of the nitrogen-phosphorus synergist in facilities vegetable.
10. application as claimed in claim 9, characterized in that synergist amount of application in facilities vegetable is 30-40kg/
Mu.
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CN116349677A (en) * | 2023-03-20 | 2023-06-30 | 寿光市新世纪种苗有限公司 | A preparation for improving low-temperature and low-light tolerance of tomato and its preparation method and application method |
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CA2977331A1 (en) * | 2015-02-25 | 2016-09-01 | Nichem Solutions | Plant growth promoting composition and a process of preparing the same |
CN108129204A (en) * | 2018-01-24 | 2018-06-08 | 山东省农业科学院农业资源与环境研究所 | A kind of nitrogen-phosphorus synergist of the hydrolyzate containing earthworm and its preparation method and application |
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CA2977331A1 (en) * | 2015-02-25 | 2016-09-01 | Nichem Solutions | Plant growth promoting composition and a process of preparing the same |
CN108129204A (en) * | 2018-01-24 | 2018-06-08 | 山东省农业科学院农业资源与环境研究所 | A kind of nitrogen-phosphorus synergist of the hydrolyzate containing earthworm and its preparation method and application |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115093855A (en) * | 2022-06-23 | 2022-09-23 | 北京晋荣农业科技有限公司 | Multi-element soil conditioner and preparation method and application thereof |
CN115093855B (en) * | 2022-06-23 | 2023-08-25 | 北京晋荣农业科技有限公司 | Multielement soil conditioner and preparation method and application thereof |
CN116349677A (en) * | 2023-03-20 | 2023-06-30 | 寿光市新世纪种苗有限公司 | A preparation for improving low-temperature and low-light tolerance of tomato and its preparation method and application method |
CN116349677B (en) * | 2023-03-20 | 2025-06-27 | 寿光市新世纪种苗有限公司 | A preparation for improving low temperature and weak light tolerance of tomatoes and its preparation method and application method |
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