CN110055179B - Carbon dioxide incubator for bioengineering - Google Patents

Abstract

The invention discloses a carbon dioxide incubator for bioengineering, comprising a first box body and a first sealing door arranged on the side wall of the first box body. A display screen and an operation panel are arranged on the equipment box, a second box is arranged in the first box, and a plurality of first chutes are arranged on the inner wall of the second box. A carrying plate is provided, a second chute is provided on the inner wall of the first box, a first slider matched with the second chute is provided on the side wall of the second box, and the second The bottom of the chute is provided with a driving member for pushing the first slider upward, and the side wall of the first slider is provided with a sealing member; through the up and down movement of the second box relative to the first box, the The volume of the incubator can be adjusted appropriately to meet the requirements of culturing different numbers of cells and improve the scope of application of the incubator.

Description

A carbon dioxide incubator for bioengineering

technical field

The invention belongs to the technical field of biological engineering, in particular to a carbon dioxide incubator for biological engineering.

Background technique

The carbon dioxide incubator is used for culturing cells by simulating a growth environment similar to that of cells/tissues in vivo in the incubator. The incubator requires stable temperature (37°C), stable CO2 level (5%), constant pH value (pH value: 7.2-7.4), and high relative saturated humidity (95%). The quality of the results directly affects the success or failure of subsequent experiments. The volume of the existing incubator is constant, but due to the different needs of the cultivation, the number of each cultivation will be changed according to the actual situation, so there will be a problem that most of the space in the incubator is not used and the space in the incubator is not used enough. problems that affect the practicability of the incubator.

SUMMARY OF THE INVENTION

In order to overcome the deficiencies of the prior art, the present invention provides a carbon dioxide incubator for biological engineering.

In order to achieve the above purpose, the present invention adopts the following technical solutions: a carbon dioxide incubator for bioengineering, comprising a first box body and a first sealing door arranged on the side wall of the first box body, the first box body There is an equipment box on one side, a display screen and an operation panel are arranged on the equipment box, a second box is arranged in the first box, and a plurality of first chutes are arranged on the inner wall of the second box , the first chute is provided with a bearing plate, the inner wall of the first box is provided with a second chute, and the side wall of the second box is provided with a second chute that matches the second chute A sliding block, the bottom of the second sliding slot is provided with a driving member for pushing the first sliding block to move upward, and a sealing member is provided on the side wall of the first sliding block; the cells or tissues are placed on the carrier plate When culturing less cells, push the first slider into the bottom of the second chute, so that the second box moves to the bottom of the first box, and the side walls of the first box and the second box They overlap each other and reduce the height of the incubator, thereby reducing the volume of the incubator, making it easier to control the interior after the volume of the incubator is reduced, and it is convenient to maintain the balance of carbon dioxide concentration, temperature and humidity in the incubator; When cells are used, the driving member drives the second box to move upward, so that the first slider moves to the top of the second chute, the side walls of the first box and the second box are no longer overlapped, and the height of the incubator is increased. The space inside the incubator allows more space to place the carrier plate in the incubator, which is convenient for culturing more cells in the incubator; through the up and down movement of the second box relative to the first box, the volume of the incubator is affected Make appropriate adjustments to meet the requirements of culturing different numbers of cells and improve the scope of application of the incubator; the seal seals the contact point between the first slider and the second chute, and cooperates with the first sealing door in the incubator Form a good sealed environment to prevent the outside air from entering the incubator and affect the steady state of the incubator, so that the indicators in the incubator meet the culture standards, increase the survival rate of cell culture, and improve the culture effect in the incubator .

The bottom of the first box is provided with a guide plate, the side wall of the second box is provided with a first movable cavity matched with the guide plate, and the inner wall of the guide plate is provided with a plurality of holes matched with the carrying plate The third chute; the guide plate cooperates with the first movable cavity to provide a guiding function for the second box, so that the second box can move up and down more stably, and improve the stability of the movement of the second box; when the second box After the body moves to the top of the first box, load the carrier plate into the third chute, increase the number of carrier plates in the incubator, facilitate the cultivation of more cells in the incubator, and improve the scope of application of the incubator; In cooperation with the first movable chamber, the sealing effect between the first box body and the second box body is increased, so as to prevent the air entering the incubator from affecting the steady state in the incubator, and ensure the cultivation effect in the incubator.

The first sliding block is provided with a second movable cavity, the second movable cavity is provided with a limit block, the inner wall of the second movable cavity is provided with a fourth chute, and the side wall of the limit block is provided with a fourth chute. There is a second slider matched with the fourth chute, a limit spring is arranged on the side wall of the second slider, and a rubber pad is arranged at one end of the limit block; when the position of the second box is not determined, After moving again, the limit block protrudes from the second movable cavity, so that the rubber pad at one end of the limit block is in contact with the inner wall of the second chute. The contact area improves the sealing effect of the first slider and the second chute, and at the same time, under the action of the rubber pad, the friction between the first slider and the inner wall of the second chute is increased, the burden of the driving part is reduced, and the second box The body is more stably placed on the top of the first box to ensure the reliability of the use of the incubator; under the action of the limit spring, the limit block has a tendency to move to the outside of the second movable cavity, so that the limit block can be used for the first The inner wall of the second chute produces a squeezing effect, which increases the contact effect between the rubber pad and the inner wall of the second chute, and further improves the sealing effect of the connection between the first slider and the second chute.

A third movable cavity is arranged on the side wall of the second box body, the third movable cavity is communicated with the second movable cavity, and a through hole is arranged on the side wall of the third movable cavity, and the through hole is arranged in the through hole. A rotating shaft is passed through, the rotating shaft is rotatably connected to the inner wall of the third movable cavity, a ring groove is arranged on the rotating shaft, a connecting rope is wound around the ring groove, and one end of the connecting rope is arranged on the inner wall of the third movable cavity. When adjusting the volume of the incubator, rotate the rotating shaft, and wind the connecting rope around the rotating shaft. After the length of the connecting rope is reduced, pull the limiting block to move into the second movable cavity, so that the limiting block and the The inner wall of the second chute is disengaged, making it easier for the driving element to push the first slider to move in the second chute, and adjusting the volume of the incubator to make the incubator adapt to the cultivation needs of different feeding materials; After moving to the proper position, release the rotating shaft, the limit spring pushes the limit block to move outward, so that the limit block pulls the connecting rope to move, the rotating shaft automatically rotates under the driving of the connecting rope, so that the limit block and the inner wall of the second chute automatically form a match , cooperates with the driving member to fix the second box body, and enhances the sealing cooperation effect between the second box body and the first box body.

One end of the rotating shaft is provided with a turntable, the turntable is provided with a gap, a fifth chute is provided on the side wall of the second box, and a third sliding block is provided in the fifth chute, and the third The slider is provided with a limit plate matched with the notch; the rotating shaft is easier to rotate under the setting of the turntable, so as to facilitate the control of the limit block; when the position of the second box is adjusted, the turntable is rotated to The connecting rope is wound around the rotating shaft to drive the limit block to move into the second movable cavity, and then the limit plate is inserted into the gap to fix the turntable, so that the position adjustment of the second box can be completed under the action of the driving member. ; When the position of the second box is determined, the limit plate is taken out from the notch, and the rotating shaft is automatically rotated under the action of the limit spring, so that the limit block is in contact with the inner wall of the second chute, and then the limit plate is moved. It is inserted into the gap, and under the action of the limit plate, it fixes the turntable, prevents the turntable from rotating and drives the limit block to move, and ensures the matching effect of the limit block and the second chute.

The first slider is provided with a through cavity, the top of the through cavity is provided with an air inlet nozzle, the two ends of the through cavity are respectively provided with first grooves, and the sealing member is arranged in the first grooves When the position of the second box body is determined, the air is filled into the through cavity from the air inlet, so that the air enters the air bag, and the air bag is made by the air pressure. The bulge is deformed, and the air bag becomes larger and contacts the inner wall of the second chute, and the gap between the first slider and the second chute is blocked to prevent the outside air from entering the incubator, and cooperates with the limit block. The first sliding block plays a fixed role to ensure the matching effect of the first box body and the second box body.

The bottom of the first box body is provided with a cavity, and the cavity is provided with a receiving cavity assembly, the receiving cavity assembly includes a top plate, a bottom plate and a sealing film, and the top plate and the bottom plate are connected by a first connecting rod , there are multiple first connecting rods, and every two first connecting rods are crossed to form a set of supporting components, one end of the first connecting rod is hinged on the top plate, and the other end is hinged on the bottom plate ; When it is necessary to take the receiving plate out of the incubator, pull the receiving cavity assembly out of the cavity, place the receiving cavity on the side of the first sealing door, and pull the top plate upward to separate the bottom plate and the top plate, and the first The connecting rod provides support for the top plate, forms a cavity between the bottom plate and the top plate, fills carbon dioxide between the bottom plate and the top plate, and adjusts the environmental parameters in the receiving cavity, so that the indicators in the receiving cavity are the same as those in the incubator. Then push the receiving plate into the receiving cavity, and the receiving cavity has an excessive effect on the taking of the receiving plate, so as to avoid the direct opening of the incubator and the impact of air entering the incubator on the culture environment in the incubator; due to the receiving cavity The space is small, which makes it easy to adjust the environment in the receiving cavity and re-regulate the environment in the culture cavity, so that the culture cavity is in a relatively stable environment, and the survival rate of cell culture is improved; the receiving cavity components are stored through the cavity , so that the receiving cavity assembly is placed in the cavity when not in use, reducing the space occupied by the culture cavity and making the use of the incubator more convenient.

The sealing film is made of elastic material, the bottom plate is provided with a plurality of support springs, the side wall of the bottom plate is provided with a connecting film, the connecting film is made of elastic material, and the other end of the connecting film is fixedly connected on the top plate; the support spring supports the top plate, when the receiving cavity assembly is taken out from the cavity, the top plate is pushed upwards under the action of the support spring, so that the top plate and the bottom plate are separated, and the connecting film is connected to the receiving cavity. The side wall plays a sealing role, making the receiving cavity form a sealed space, which is convenient to control the environment in the receiving cavity; through the arrangement of elastic materials, the sealing film and the connecting film are stretched when the top plate moves, so that the connecting plate and the sealing film are stretched. Always connect with the top plate and bottom plate, and have a good sealing effect on the receiving cavity.

The first sealing door is provided with a fourth movable cavity, the fourth movable cavity is provided with a second sealing door, and the first sealing door is provided with a plurality of first passages matched with the receiving plate. The second sealing door is provided with a second through groove matched with the first through groove, the first through groove is provided with a first baffle plate, and the second through groove is provided with a second through groove. Two baffles; when the second sealing door is placed in the fourth movable chamber, the first through groove and the second through groove are aligned. When one of the carrier plates in the culture chamber needs to be taken, the receiving chamber The component is taken out from the cavity, the receiving cavity component is moved to the side of the corresponding receiving plate position, and the environment in the receiving cavity is adjusted so that the environment in the receiving cavity is the same as the environment in the culture The first baffle plate and the second baffle plate are opened, and the receiving plate is pushed out from the first through groove and the second through groove, so that the receiving plate is placed in the receiving cavity. The plate and the second baffle are closed, the sealing film is opened, and the receiving plate is taken out from the receiving cavity, so as to pick up the cells on the receiving plate, so as to avoid air directly entering the incubator when one of the receiving plates is taken. The environment in the incubator has an impact, ensuring a stable environment in the incubator and improving the cell survival rate.

A fourth sliding block is arranged on the side wall of the top plate, and a fifth sliding block is arranged on the side wall of the bottom plate. The structure of the fourth sliding block is the same as that of the fifth sliding block. There is a sixth chute matched with the fourth slider, and a seventh chute matched with the fourth slider is provided on the second sealing door; under the cooperation of the slider and the chute , so that the receiving chamber assembly can move along the surface of the sealing door, so that the sealing chamber assembly can be moved to the corresponding position on the sealing door according to the needs, which is convenient for taking out the receiving plate everywhere in the incubator and improving the convenience of using the incubator. .

The invention has the following advantages: through the up-and-down movement of the second box relative to the first box, the volume of the incubator can be properly adjusted to meet the requirements for culturing different numbers of cells and improve the scope of application of the incubator.

Description of drawings

FIG. 1 is a schematic structural diagram of the present invention.

FIG. 2 is a schematic cross-sectional view 1 of the present invention.

FIG. 3 is an enlarged view of part A in FIG. 2 .

FIG. 4 is an enlarged view of B in FIG. 2 .

FIG. 5 is a second schematic cross-sectional view of the present invention.

FIG. 6 is an enlarged view of C in FIG. 5 .

FIG. 7 is a third schematic cross-sectional view of the present invention.

FIG. 8 is an enlarged view of D in FIG. 7 .

FIG. 9 is a schematic cross-sectional view four of the present invention.

FIG. 10 is an enlarged view of E in FIG. 9 .

FIG. 11 is a schematic cross-sectional view V of the present invention.

FIG. 12 is an enlarged view of F in FIG. 11 .

Detailed ways

As shown in Figures 1-12, a carbon dioxide incubator for bioengineering includes a first box body 1 and a first sealed door 3 provided on the side wall of the first box body 1. The first box body 1 One side is provided with an equipment box 4, the equipment box 4 is provided with a display screen and an operation panel, the display panel displays various parameters in the incubator, and the operation panel is provided with a plurality of buttons for controlling the operation in the incubator. Various movements, the basic structure of the carbon dioxide incubator is the same as the structure of the carbon dioxide incubator in the prior art, including various sensors and air supply systems, which will not be repeated here; Two boxes 2 , a plurality of first chutes are arranged on the inner wall of the second box 2 , a bearing plate 21 is arranged in the first runners, and the inner walls of both sides of the first box 1 are respectively provided with The second chute 11, the side wall of the second box body 2 is provided with a first sliding block 22 matched with the second chute 11, and the bottom of the second chute 11 is provided for pushing the first sliding block 22. A driving member for the upward movement of the slider 22, a sealing member is provided on the side wall of the first slider 22; the cells or tissues are placed on the carrier plate for culture, and when fewer cells are cultured, the first slider Push it into the bottom of the second chute, so that the second box moves to the bottom of the first box, the side walls of the first box and the second box overlap each other, reducing the height of the incubator, thereby reducing the volume of the incubator , it is easier to control the interior after the volume of the incubator is reduced, and it is convenient to maintain the balance of carbon dioxide concentration, temperature and humidity in the incubator; when culturing more cells, the driving part drives the second box to move upward, so that the first A slider moves to the top of the second chute, the side walls of the first box and the second box are no longer overlapped, increasing the height of the incubator, thereby increasing the space inside the incubator, so that there is more space in the incubator to place The carrier plate is convenient for culturing more cells in the incubator; through the up and down movement of the second box relative to the first box, the volume of the incubator can be adjusted appropriately to meet the requirements of culturing different numbers of cells, and the incubator can be improved The scope of application; the seal seals the contact point between the first slider and the second chute, and cooperates with the first sealing door to form a good sealing environment in the incubator to prevent the outside air from entering the incubator. The steady state in the incubator has an impact, so that the indicators in the incubator meet the culture standards, increase the cell culture survival rate, and improve the culture effect in the incubator.

The driving member is a pneumatic rod 12 arranged at the bottom of the second chute. A third through slot is provided on the side wall of the second chute, and a second connecting rod 13 is passed through the third through slot. There is a push rod on the rod, and the second connecting rod is connected with the switch of the air pressure rod; under the action of the second connecting rod, the switches of the air pressure rod in the second chute on the left and right sides are connected to the push rod, so that the push rod can be pushed When the rod is pressed down, the two air pressure rods are operated at the same time; when the second box needs to be raised, the push rod is pressed down, and the switches of the two air pressure rods are turned on to push the first slider to move up, so that the second box The body rises up to increase the space of the incubator; when the second box needs to be pressed into the first box, press the push rod and press the second box down, so that the first slider slides along the second box. The tank moves downward, and the second box is pressed into the first box, which reduces the space of the incubator, avoids the waste of resources caused by the excessive space of the incubator, and makes the environmental regulation of the incubator more convenient.

The bottom of the first box body 1 is provided with a guide plate 14, the side wall of the second box body 2 is provided with a first movable cavity matched with the guide plate 14, and the inner wall of the guide plate 14 is provided with a plurality of The guide plate cooperates with the first movable cavity to provide a guiding function for the second box body, so that the second box body can be moved more stably, and the stability of the movement of the second box body can be improved. After the second box moves to the top of the first box, load the carrier plate into the third chute, increase the number of carrier plates in the incubator, facilitate the cultivation of more cells in the incubator, and improve the incubator. The scope of application; under the cooperation of the guide plate and the first movable cavity, the sealing effect between the first box and the second box is increased to prevent the air entering the incubator from affecting the steady state of the incubator and ensure the cultivation of Cultivation effect in the box.

The second chute and the third chute are both provided with drive wheels, the first box body and the second box body are provided with drive motors that are driven and matched with the drive wheels, and the receiving plate is placed in the second chute or the third chute. When inside the chute, the transmission wheel is in contact with the side wall of the receiving plate, and when the driving motor drives the transmission wheel to rotate, the receiving plate can be pushed out from the second or third chute.

The first slider 22 is provided with a second movable cavity, the second movable cavity is provided with a limit block 26, the second movable cavity penetrates the first slider, and the limit blocks are divided into two groups, which are respectively arranged in the second At both ends of the movable cavity, the inner wall of the second movable cavity is provided with a fourth chute 221, and the side wall of the limit block 26 is provided with a second sliding block 261 matched with the fourth chute 221, so A limit spring 262 is provided on the side wall of the second slider 261, and a rubber pad is provided at one end of the limit block 26; when the position of the second box is determined and no longer moves, the limit block extends from the second movable cavity out, the rubber pad at one end of the limit block is in contact with the inner wall of the second chute. Under the action of the rubber pad, the contact area between the limit block and the second chute is increased, and the contact area between the first slider and the second chute is increased. At the same time, under the action of the rubber pad, the friction between the first slider and the inner wall of the second chute is increased, the burden of the driving part is reduced, and the second box is more stably placed on the top of the first box to ensure the cultivation of The reliability of the box is improved; under the action of the limit spring, the limit block has a tendency to move to the outside of the second movable cavity, so that the limit block has a squeezing effect on the inner wall of the second chute, increasing the rubber pad and the first The contact effect of the inner walls of the second chute further enhances the sealing effect of the connection between the first slider and the second chute.

The side wall of the second box body 2 is provided with a third movable cavity 23, the third movable cavity 23 communicates with the second movable cavity, and the side wall of the third movable cavity 23 is provided with a through hole, so the third movable cavity 23 communicates with the second movable cavity. A rotating shaft 24 is pierced through the through hole, and the rotating shaft 24 is rotatably connected to the inner wall of the third movable cavity 23. The rotating shaft 24 is provided with a ring groove 241, and a connecting rope is wound around the ring groove 241. , one end of the connecting rope is set on the limiting block 26; when adjusting the volume of the incubator, the rotating shaft is rotated, the connecting rope is wound on the rotating shaft, and the length of the connecting rope is reduced, and the limiting block is pulled to the first The movement in the second movable cavity makes the limit block disengage from the inner wall of the second chute, so that the driving element can more easily push the first slider to move in the second chute, adjust the volume of the incubator, and make the incubator adapt to different Cultivation requirements for feeding materials; after the second box moves to an appropriate position, the rotating shaft is released, and the limit spring pushes the limit block to move outward, so that the limit block pulls the connecting rope to move, and the rotating shaft is automatically driven by the connecting rope to rotate, so that the limit The position block automatically forms cooperation with the inner wall of the second chute, and cooperates with the driving member to fix the second box body, thereby improving the sealing effect of the second box body and the first box body.

One end of the rotating shaft 24 is provided with a turntable 242, and the turntable 242 is provided with a notch. The third slider is provided with a limit plate 25 which is matched with the notch; under the setting of the turntable, the rotating shaft is easier to rotate, so as to facilitate the control of the limit block; when the position of the second box is adjusted When the turntable is rotated, the connecting rope is wound around the rotating shaft to drive the limit block to move into the second movable cavity, and then the limiter plate is inserted into the gap to fix the turntable, so that the second movement can be easily completed under the action of the driving member. The position of the box body is adjusted; when the position of the second box body is determined, the limit plate is taken out from the notch, and the rotating shaft is automatically rotated under the action of the limit spring, so that the limit block is in contact with the inner wall of the second chute. Then, the limit plate is inserted into the gap, and under the action of the limit plate, the turntable is fixed to prevent the rotation of the turntable from driving the limit block to move, so as to ensure the matching effect of the limit block and the second chute.

The first slider 22 is provided with a through cavity 222, and the top of the through cavity 222 is provided with an air intake nozzle. The air intake nozzle has the same structure as the air intake nozzle of a bicycle tire in the prior art, and will not be repeated here. , the two ends of the through-cavity 222 are respectively provided with first grooves, the sealing member is an airbag 223 arranged in the first groove, and the airbag 223 communicates with the through-cavity 222; the airbag is made of latex After the position of the second box is determined, the air is filled into the through cavity from the air inlet, so that the air enters the air bag, and the air bag is bulged and deformed under the action of the air pressure. The inner walls of the two chutes are in contact with each other, and the gap between the first sliding block and the second sliding groove is blocked to prevent the outside air from entering the incubator. The matching effect of the body and the second box body.

The bottom of the first box body 1 is provided with a cavity, and the cavity is provided with a receiving cavity assembly. The receiving cavity assembly includes a top plate 51, a bottom plate 52 and a sealing film 56, and the top plate 51 and the bottom plate 52 pass through. A first connecting rod 55 is connected to each other. There are multiple first connecting rods 55. Every two first connecting rods 55 are crossed to form a set of supporting components. One end of the first connecting rod 55 is hinged to the top plate. 51, the other end is hinged on the bottom plate 52; there is a push block on the side wall of the bottom plate, which is convenient for pulling the bottom plate out of the cavity; when the receiving plate needs to be taken out of the incubator, the receiving cavity assembly is Pull it out of the cavity, so that the receiving cavity is placed on the side of the first sealing door, and the top plate is pulled upward to separate the bottom plate and the top plate. The first connecting rod provides a supporting force for the top plate, forming a cavity between the bottom plate and the top plate. Carbon dioxide is filled between the bottom plate and the top plate, and the environmental parameters in the receiving chamber are adjusted so that the indicators in the receiving chamber are the same as those in the incubator, and then the receiving plate is pushed into the receiving Taking it will play an excessive role, avoiding the direct opening of the incubator and causing the air to enter the incubator and affecting the culture environment in the incubator; due to the small space of the receiving cavity, it is easy to adjust the environment in the receiving cavity. The environment regulation of the cultivating chamber can keep the culture chamber in a relatively stable environment and improve the survival rate of cell culture; the receiving chamber components are stored through the cavity, so that the receiving chamber components can be placed in the cavity when not in use, reducing the need for culture. The space occupied by the cavity makes the use of the incubator more convenient; the top of the sealing film is fixed on the top plate, the bottom of the sealing film is provided with an iron ring, the bottom plate is provided with a vertical rod, and the iron ring on the sealing film is sleeved on the vertical rod , so that the sealing film is connected with the bottom plate and the top plate respectively.

The sealing film 56 is made of rubber, the bottom plate 52 is provided with a plurality of support springs 54, the side wall of the bottom plate 52 is provided with a connecting film 53, the connecting film 53 is made of rubber, and the connecting film 53 is made of rubber. The other end of 53 is fixedly connected to the top plate 51; the connecting film and the sealing film seal the three sides of the receiving cavity, and the side of the receiving cavity in contact with the sealing door is in a conducting state, which is convenient for the receiving plate to enter the receiving cavity; The top plate plays a supporting role. When the receiving cavity assembly is taken out from the cavity, the top plate is pushed upward under the action of the support spring, so that the top plate and the bottom plate are separated. A sealed space is formed, which is convenient to control the environment in the receiving cavity; through the setting of elastic materials, the sealing film and the connecting film are stretched when the top plate moves, so that the connecting plate and the sealing film are always connected with the top plate and the bottom plate. The receiving cavity has a good sealing effect.

The first sealing door 3 is provided with a fourth movable cavity, the fourth movable cavity is provided with a second sealing door 32 , and the first sealing door 3 is provided with a plurality of matching plates 21 . The first through groove is provided, the second sealing door 32 is provided with a second through groove matched with the first through groove, the first through groove is provided with a first baffle 31, the second through groove A second baffle 33 is arranged in the through groove; both the first sealing door and the second sealing door are provided with a cylinder group for controlling the opening and closing of the first baffle and the second baffle. The number of chutes corresponds, and the first through groove is set on one side of the first sliding groove; when the second sealing door is placed in the fourth movable cavity, the first through groove and the second through groove are aligned. When taking one of the bearing plates in the culture chamber, take the bearing chamber assembly out of the cavity, move the bearing chamber assembly to the side of the corresponding bearing plate position, and adjust the environment in the bearing chamber so that the The environment is the same as the environment in the culture chamber. The first baffle and the second baffle corresponding to one side of the receiving plate are opened, and the receiving plate is pushed out from the first through groove and the second through groove, so that the receiving plate is placed in the receiving chamber. Inside, when the receiving plate completely enters the receiving cavity, the first baffle and the second baffle are closed, the sealing film is opened, and the receiving plate is taken out from the receiving cavity, so as to pick up the cells on the receiving plate and avoid taking it out. When one of the receiving plates is taken, the air directly enters the incubator, which will affect the environment in the incubator, ensure a stable environment in the incubator, and improve the cell survival rate.

A fourth sliding block is arranged on the side wall of the top plate 51, and a fifth sliding block is arranged on the side wall of the bottom plate 52. The structure of the fourth sliding block is the same as that of the fifth sliding block. The first sealing door 3 is provided with a sixth chute 34 matched with the fourth sliding block, and the second sealing door 32 is provided with a seventh chute 35 matched with the fourth sliding block; With the mutual cooperation of the chutes, the receiving chamber assembly can move along the surface of the sealing door, so that the sealing chamber assembly can be moved to the corresponding position on the sealing door as required, and it is convenient to take out the receiving plate everywhere in the incubator and lift it. Ease of use of the incubator.

A disinfection lamp is arranged on the top plate, and a second groove matched with the disinfection lamp is arranged on the bottom plate.

Adjust the position of the second box according to the number of cells to be cultured. Take the second box rising to the top of the first box as an example, take the limit plate out of the gap, and turn the turntable to make the limit block enter the In the second movable cavity, insert the limit plate into the notch; press the push rod down, the air pressure rod pushes the first sliding block upwards, when the first sliding block moves to the top of the second chute, release the pusher lever to take out the limit plate from the notch, the limit spring pushes the limit block to contact the inner wall of the second chute, when the limit block contacts the inner wall of the second chute, insert the limit plate into the gap, and go to the second chute. Air is introduced into the air inlet, and the air bag is filled with air and then bulged to contact the inner wall of the second chute, sealing the connection between the first slider and the second chute; put the cells to be cultured into the matching to undertake Then push the receiving plate into the first chute and the third chute, the second sealing door protrudes from the fourth movable cavity, cover the first sealing door and the second sealing door, and carry out the operation of the incubator. It is sealed, and the environment in the incubator is controlled by the control plate, so that the indicators in the incubator are in a standard state, and the cells are cultured; when one of the carrier plates needs to be taken out, the bottom plate is pulled out from the cavity, Under the action of the limit spring, the top plate is pushed up, and the top plate and the bottom cooperate with each other to form a receiving cavity, and the receiving cavity is moved along the chute, so that the receiving cavity moves to the side of the receiving plate that needs to be taken out. Adjust so that the indicators in the receiving cavity are the same as those in the incubator. After the baffle is opened, the receiving plate is pushed out of the through groove under the action of the transmission wheel, and the receiving plate is pushed into the receiving cavity. After entering the receiving chamber completely, close the baffle, open the sealing film, and take out the receiving plate from the receiving chamber to prevent the air entering the incubator from affecting the cells on other receiving plates; when it is necessary to put the receiving plate again. When entering the incubator, put the receiving plate into the receiving cavity, close the sealing film, adjust the environment in the receiving cavity so that the environment in the receiving cavity is the same as that in the culture chamber, turn on the disinfection lamp, and sterilize the receiving plate. The baffle is opened, and the receiving plate is pushed into the through groove, and the receiving plate is entered into the incubator under the action of the transmission wheel, and then the baffle is closed; Push it to one side of the cavity, press down the top plate to keep the support spring in a compressed state, push the top plate and bottom plate into the cavity, and accommodate the receiving cavity, reduce the space occupied by the incubator, and facilitate the use of the incubator.

Claims (1)

1. The utility model provides a carbon dioxide incubator for bioengineering, includes first box (1) and locates first sealing door (3) on the first box (1) lateral wall, first box (1) one side is equipped with equipment box (4), be equipped with display screen and operation panel on equipment box (4), its characterized in that: a second box body (2) is arranged in the first box body (1), a plurality of first sliding grooves are formed in the inner wall of the second box body (2), a bearing plate (21) is arranged in each first sliding groove, a second sliding groove (11) is formed in the inner wall of the first box body (1), a first sliding block (22) matched with the second sliding groove (11) is arranged on the side wall of the second box body (2), a driving piece used for pushing the first sliding block (22) to move upwards is arranged at the bottom of the second sliding groove (11), and a sealing piece is arranged on the side wall of the first sliding block (22);

a guide plate (14) is arranged at the bottom of the first box body (1), a first movable cavity matched with the guide plate (14) is arranged on the side wall of the second box body (2), and a plurality of third sliding grooves matched with the bearing plate (21) are formed in the inner wall of the guide plate (14);

a second movable cavity is arranged in the first sliding block (22), a limiting block (26) is arranged in the second movable cavity, a fourth sliding groove (221) is formed in the inner wall of the second movable cavity, a second sliding block (261) matched with the fourth sliding groove (221) is arranged on the side wall of the limiting block (26), a limiting spring (262) is arranged on the side wall of the second sliding block (261), and a rubber pad is arranged at one end of the limiting block (26);

a third movable cavity (23) is formed in the side wall of the second box body (2), the third movable cavity (23) is communicated with the second movable cavity, a through hole is formed in the side wall of the third movable cavity (23), a rotating shaft (24) penetrates through the through hole, the rotating shaft (24) is rotatably connected to the inner wall of the third movable cavity (23), an annular groove (241) is formed in the rotating shaft (24), a connecting rope is wound on the annular groove (241), and one end of the connecting rope is arranged on the limiting block (26);

a rotating disc (242) is arranged at one end of the rotating shaft (24), a notch is formed in the rotating disc (242), a fifth sliding groove is formed in the side wall of the second box body (2), a third sliding block is arranged in the fifth sliding groove, and a limiting plate (25) matched with the notch is arranged on the third sliding block;

a through cavity (222) is formed in the first sliding block (22), an air inlet nozzle is arranged at the top of the through cavity (222), first grooves are formed in two ends of the through cavity (222), the sealing element is an air bag (223) arranged in the first grooves, and the air bag (223) is communicated with the through cavity (222);

the bottom of the first box body (1) is provided with a cavity, a bearing cavity assembly is arranged in the cavity and comprises a top plate (51), a bottom plate (52) and a sealing film (56), the top plate (51) and the bottom plate (52) are connected through a first connecting rod (55), the number of the first connecting rods (55) is multiple, every two first connecting rods (55) are arranged in a crossed mode to form a group of supporting assemblies, one end of each first connecting rod (55) is hinged to the top plate (51), and the other end of each first connecting rod (55) is hinged to the bottom plate (52);

the sealing film (56) is made of elastic materials, a plurality of supporting springs (54) are arranged on the bottom plate (52), a connecting film (53) is arranged on the side wall of the bottom plate (52), the connecting film (53) is made of elastic materials, and the other end of the connecting film (53) is fixedly connected to the top plate (51);

a fourth movable cavity is arranged in the first sealing door (3), a second sealing door (32) is arranged in the fourth movable cavity, a plurality of first through grooves matched with the bearing plate (21) are formed in the first sealing door (3), a second through groove matched with the first through grooves is formed in the second sealing door (32), a first baffle (31) is arranged in the first through groove, and a second baffle (33) is arranged in the second through groove;

the novel sealing door is characterized in that a fourth sliding block is arranged on the side wall of the top plate (51), a fifth sliding block is arranged on the side wall of the bottom plate (52), the fourth sliding block is identical to the fifth sliding block in structure, a sixth sliding groove (34) matched with the fourth sliding block is formed in the first sealing door (3), and a seventh sliding groove (35) matched with the fourth sliding block is formed in the second sealing door (32).

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