CN110051544A - Environment protection biological cellulose mask matrix and preparation method thereof - Google Patents

Abstract

The present invention relates to a kind of environment protection biological cellulose mask matrixes and preparation method thereof, the environment protection biological cellulose mask matrix is prepared by laxative remedy: 1) banana skin of fresh clean taken, adds to blend in pulper after water and is slurried, aerobic fermentation, filtering, obtains required banana skin extracting solution；2) using banana skin extracting solution as culture medium, culture medium is moved into mold, gluconate pyracetobacillus is inoculated with, obtains biology cellulose membrane matrix by culture.The present invention develops and uses banana skin as the Major Nutrient substance in microbiological culture media in a creative way, sufficiently excavates the potential value of banana skin, and carry out wasted resources turns waste into wealth to reduce the wasting of resources.Production technology of the present invention can be effectively reduced the energy consumption in existing facial mask production technology and the pollution to environment, create higher economic benefit.

Description

Environment-friendly biocellulose mask substrate and preparation method thereof

technical field

The invention relates to the use of banana peel fermentation to prepare a biocellulose facial mask matrix, which belongs to the reuse of waste gas in the fruit processing industry to the microbial fermentation industry, and the produced biocellulose facial mask matrix can be used as a raw material for daily cosmetics.

Background technique

Bacterial cellulose (BC for short) is an extracellular product produced by microorganisms such as Acetobacter and Salmonella. It is a kind of linear polymer synthesized by fermentation of microorganisms. BC is the same as plant-derived cellulose in chemical composition and basic composition, both of which are polymerized by D-glucopyranose through β-1,4 glycosidic bond. It is a natural polymer with excellent biocompatibility and degradability. thing. Unlike general plant-derived cellulose, which is mixed with impurities such as hemicellulose and lignin, BC has a higher chemical purity due to its single composition, and its fiber structure is more regular. Bacterial cellulose has a nano-scale ultra-fine mesh Its pore-rich three-dimensional network structure endows it with high permeability and high water holding capacity, as well as biocompatibility. In the laboratory, Acetobacter xylinum can be cryopreserved, inoculated and subcultured at any time, and cultured to produce BC.

my country is a big country in banana planting and production, with an annual output of more than 12 million tons. At present, the use of bananas in the banana processing industry only stays on the utilization of the pulp, and the banana peels, which account for 30%-40% of the banana mass, or 3.6-4.8 million tons, are usually discarded directly as garbage, resulting in a waste of resources. After testing, banana peel contains a large amount of pectin, oligosaccharides, protein, water-soluble sugar, fat, various vitamins and inorganic salts and other components, which is very suitable as a medium for microorganisms. Therefore, the project team pioneered the development and utilization of banana peel as the main nutrient in the microbial medium, and cultivated Acetobacter xylinum to produce Bacterial cellulose (BC) with high market value, thereby reducing resource waste. ,Turning waste into treasure. This project fully explores the potential value of banana peels and utilizes waste resources. The new process developed can effectively reduce the energy consumption and environmental pollution in the existing mask production process, and create higher economic benefits.

To sum up, the use of biocellulose as the carrier of the shaped facial mask has unique advantages. The present invention develops and utilizes a large number of discarded banana peels produced by my country's banana processing industry, and uses it as the main nutritional raw material required for biofermentation to prepare biomaterials. Cellulose mask can not only reduce the waste of banana peel resources of about 4 million tons per year, improve its comprehensive utilization value, but also produce good economic and social effects.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a method for recycling waste, banana peel, and using it to produce a biocellulose mask matrix for high-end skin care products.

The technical scheme provided by the invention is: the preparation method of the environment-friendly biological cellulose facial mask matrix comprises the following steps:

1) Take fresh and clean banana peels, grind them in a grinder for 20 minutes to form pulp, ferment naturally for 24 hours, and then filter the residue in the banana peel pulp with gauze to obtain the desired banana peel extract;

2) The banana peel extract is moved into the mold as a culture medium, inoculated with Acetobacter xylinum, and a biocellulose film is obtained after 8-10 days;

3) Boil the biocellulose film obtained in step 2) in a 0.25 mol/L sodium hydroxide alkaline solution for 30 minutes to obtain a formed film;

4) Repeatedly brewing the formed film obtained in step 3) with water until the film becomes neutral to obtain a neutral film;

5) Cut the neutral film obtained in step 4) to obtain an environmentally friendly biocellulose facial mask matrix.

The banana peel extract and the mold in the step 2) were sterilized at 120° C. for 30 minutes before inoculation with Acetobacter xylinum.

The inoculation amount of the step 2) is 2%-6% of the volume of the medium.

The bacterial liquid inoculated in the step 2) is Acetobacter xylindronate purchased in the laboratory.

The operation during the step 2) inoculation is to first pour the banana water into the mold in a sterile environment, and then use a pipette gun to inject the bacterial liquid accounting for 2%-6% of the liquid volume in the mold.

The step 2) culture conditions are static culture at a constant temperature of 30°C.

The invention uses the discarded banana peels in the banana processing industry as raw materials, and utilizes the nutrients contained in the banana peels as the main components of the microbial culture liquid for the growth of Gluconacetobacter xylinus, so as to produce products with excellent physical properties. Biocellulose film with chemical properties, and simple treatment to obtain a biocellulose mask matrix. The biocellulose facial mask matrix obtained by the present invention can be used for preparing facial mask instead of the prior art facial mask matrix. The facial mask matrix of the present invention is immersed in the essence used for the production of ordinary facial masks to obtain a biocellulose facial mask with the use effect of the facial masks sold in the market. The biological cellulose facial mask matrix obtained by the invention has low cost and low labor consumption, and is prepared by fermenting the originally worthless and almost zero-cost banana peel by mechanically crushing it. The invention pioneers the development and utilization of banana peel as the main nutrient in the microbial culture medium, fully explores the potential value of banana peel, and utilizes waste resources, thereby reducing waste of resources and turning waste into treasure. The production process of the invention can effectively reduce the energy consumption and environmental pollution in the existing facial mask production process, and create higher economic benefits.

Detailed ways

Example 1

1) Take fresh and clean banana peels, grind them in a grinder for 20 minutes to form pulp, ferment naturally for 24 hours, and then filter the residue in the banana peel pulp with gauze to obtain the desired banana peel extract;

2) The banana peel extract is moved into the mold as a culture medium, inoculated with Acetobacter xylinum, and a biocellulose film is obtained after 8 days;

3) Boil the biocellulose film obtained in step 2) in a 0.25 mol/L sodium hydroxide alkaline solution for 30 minutes to obtain a formed film;

4) Repeatedly brewing the formed film obtained in step 3) with water to obtain a neutral film;

5) Cut the neutral film obtained in step 4) to obtain an environmentally friendly biocellulose facial mask matrix.

The banana peel extract and the mold in the step 2) were sterilized at 120° C. for 30 minutes before inoculation with Acetobacter xylinum.

The step 2) inoculation amount is 2% of the medium volume.

The bacterial liquid inoculated in the step 2) is Acetobacter xylindronate purchased in the laboratory.

The operation during the inoculation in the step 2) is to first pour the banana peel extract into the mold in a sterile environment, and then use a pipette gun to inject the bacterial liquid accounting for 2% of the liquid volume in the mold at the vertical liquid level.

The step 2) culture conditions are static culture at a constant temperature of 30°C.

Example 2

1) Take fresh and clean banana peels, grind them in a grinder for 20 minutes to form pulp, ferment naturally for 24 hours, and then filter the residue in the banana peel pulp with gauze to obtain the desired banana peel extract;

2) The banana peel extract is moved into the mold as a culture medium, inoculated with Acetobacter xylinum, and a biocellulose film is obtained after 8 days;

3) Boil the biocellulose film obtained in step 2) in a 0.25 mol/L sodium hydroxide alkaline solution for 30 minutes to obtain a formed film;

4) Repeatedly brewing the formed film obtained in step 3) with water to obtain a neutral film;

5) Cut the neutral film obtained in step 4) to obtain an environmentally friendly biocellulose facial mask matrix.

The banana peel extract and the mold in the step 2) were sterilized at 120° C. for 30 minutes before inoculation with Acetobacter xylinum.

The step 2) inoculation amount is 4% of the medium volume.

The bacterial liquid inoculated in the step 2) is Acetobacter xylindronate purchased in the laboratory.

The operation during the inoculation in the step 2) is to first pour the banana water into the mold in a sterile environment, and then inject the bacterial liquid accounting for 4% of the liquid volume in the mold with the vertical liquid surface of the pipette gun.

The step 2) culture conditions are static culture at a constant temperature of 30°C.

Example 3

1) Take fresh and clean banana peels, grind them in a grinder for 20 minutes to form pulp, ferment naturally for 24 hours, and then filter the residue in the banana peel pulp with gauze to obtain the desired banana peel extract;

2) The banana peel extract was moved into the mold as a culture medium, inoculated with Acetobacter xylinum, and a biocellulose film was obtained after 9 days;

3) Boil the biocellulose mask obtained in step 2) in a 0.25 mol/L sodium hydroxide alkaline solution for 30 minutes to obtain a formed film;

4) Repeatedly brewing the formed film obtained in step 3) with water to obtain a neutral film;

5) Cut the neutral film obtained in step 4) to obtain an environmentally friendly biocellulose facial mask matrix.

The banana peel extract and the mold in the step 2) were sterilized at 120° C. for 30 minutes before inoculation with Acetobacter xylinum.

The step 2) inoculation amount is 4% of the medium volume.

The bacterial liquid inoculated in the step 2) is Acetobacter xylindronate purchased in the laboratory.

The operation during the inoculation in the step 2) is to first pour the banana water into the mold in a sterile environment, and then inject the bacterial liquid accounting for 4% of the liquid volume in the mold with the vertical liquid surface of the pipette gun.

The step 2) culture conditions are static culture at a constant temperature of 30°C.

Example 4

1) Take fresh and clean banana peels, grind them in a grinder for 20 minutes to form pulp, ferment naturally for 24 hours, and then filter the residue in the banana peel pulp with gauze to obtain the desired banana peel extract;

2) The banana peel extract was moved into the mold as a culture medium, inoculated with Acetobacter xylinum, and a biocellulose film was obtained after 10 days;

3) Boil the biocellulose mask obtained in step 2) in a 0.25 mol/L sodium hydroxide alkaline solution for 30 minutes to obtain a formed film;

4) Repeatedly brewing the formed film obtained in step 3) with water to obtain a neutral film;

5) Cut the neutral film obtained in step 4) to obtain an environmentally friendly biocellulose facial mask matrix.

The banana peel extract and the mold in the step 2) were sterilized at 120° C. for 30 minutes before inoculation with Acetobacter xylinum.

The step 2) inoculation amount is 4% of the medium volume.

The bacterial liquid inoculated in the step 2) is Acetobacter xylindronate purchased in the laboratory.

The operation during the inoculation in the step 2) is to first pour the banana water into the mold in a sterile environment, and then inject the bacterial liquid accounting for 4% of the liquid volume in the mold with the vertical liquid surface of the pipette gun.

The step 2) culture conditions are static culture at a constant temperature of 30°C.

Example 5

1) Take fresh and clean banana peels, grind them in a grinder for 20 minutes to form pulp, ferment naturally for 24 hours, and then filter the residue in the banana peel pulp with gauze to obtain the desired banana peel extract;

2) The banana peel extract was transferred into the mold as a medium, inoculated with Acetobacter xylinum, and a biocellulose film was obtained after 10 days.

3) Boil the biocellulose mask obtained in step 2) in a 0.25 mol/L sodium hydroxide alkaline solution for 30 minutes to obtain a formed film;

4) Repeatedly brewing the formed film obtained in step 3) with water to obtain a neutral film;

5) Cut the neutral film obtained in step 4) to obtain an environmentally friendly biocellulose facial mask matrix.

The banana peel extract and the mold in the step 2) were sterilized at 120° C. for 30 minutes before inoculation with Acetobacter xylinum.

The step 2) inoculation amount is 6% of the medium volume.

The bacterial liquid inoculated in the step 2) is Acetobacter xylindronate purchased in the laboratory.

The operation during the inoculation in the step 2) is to first pour the banana water into the mold in a sterile environment, and then use a pipette gun to inject the bacterial liquid that accounts for 6% of the liquid volume in the mold at the vertical liquid level.

The step 2) culture conditions are static culture at a constant temperature of 30°C.

Example 6

1) Take fresh and clean banana peels, grind them in a grinder for 20 minutes to form pulp, ferment naturally for 24 hours, and then filter the residue in the banana peel pulp with gauze to obtain the desired banana peel extract;

2) The banana peel extract was transferred into the mold as a medium, inoculated with Acetobacter xylinum, and a biocellulose film was obtained after 10 days.

3) Boil the biocellulose mask obtained in step 2) in a 0.25 mol/L sodium hydroxide alkaline solution for 30 minutes to obtain a formed film;

4) Repeatedly brewing the formed film obtained in step 3) with water to obtain a neutral film;

5) Cut the neutral film obtained in step 4) to obtain an environmentally friendly biocellulose facial mask matrix.

The banana peel extract and the mold in the step 2) were sterilized at 120° C. for 30 minutes before inoculation with Acetobacter xylinum.

The step 2) inoculation amount is 6% of the medium volume.

The bacterial liquid inoculated in the step 2) is Acetobacter xylindronate purchased in the laboratory.

The operation during the inoculation in the step 2) is to first pour the banana water into the mold in a sterile environment, and then use a pipette gun to inject the bacterial liquid that accounts for 6% of the liquid volume in the mold at the vertical liquid level.

The step 2) culture conditions are static culture at a constant temperature of 30°C.

Take the mask matrix obtained in Example 4 for various tests.

Physical Properties of Mask Matrix Water holding rate (%) Tensile strength (Mpa) Water vapor transmission rate (g·㎡/24h) Modulus of elasticity (Mpa) 90° peeling degree (N/m) Elongation at break (%) Biocellulose Film Matrix Made from Fermentation of Banana Peel 97.30 1.13±0.10 8482 4.90±0.86 1.04 68

It can be seen that the biocellulose membrane prepared by banana peel fermentation can be put into production as a mask substrate.

Claims (7)

1. A preparation method of an environmentally friendly biocellulose facial mask matrix, comprising the following steps: 1) Take fresh and clean banana peels, grind them in a grinder for 20 minutes to form pulp, ferment naturally for 24 hours, and then filter the residue in the banana peel pulp with gauze to obtain the desired banana peel extract; 2) The banana peel extract is moved into the mold as a culture medium, inoculated with Acetobacter xylinum, and a biocellulose film is obtained after 8-10 days; 3) Boil the biocellulose film obtained in step 2) in a 0.25 mol/L sodium hydroxide alkaline solution for 30 minutes to obtain a formed film; 4) Repeatedly brewing the formed film obtained in step 3) with water until the film becomes neutral to obtain a neutral film; 5) Cut the neutral film obtained in step 4) to obtain an environmentally friendly biocellulose facial mask matrix. 2. The method for preparing an environmentally friendly biocellulose facial mask substrate according to claim 1, wherein the banana peel extract and the mold in step 2) are sterilized at 120° C. for 30 minutes before inoculation with Acetobacter xylinum . 3 . The method for preparing an environmentally friendly biocellulose facial mask according to claim 1 , wherein in the step 2) the inoculation amount is 2%-6% of the liquid volume of the medium. 4 . 4 . The method for preparing an environmentally friendly biocellulose facial mask according to claim 1 , wherein the step 2) inoculating Acetobacter xylinum gluconate refers to placing the bacterial liquid in the banana peel extract. 5 . 5. The preparation method of the environment-friendly biocellulose facial mask according to claim 1, characterized in that: the operation during the inoculation in the step 2) is to first pour the banana water into the mold in a sterile environment, and then use the The liquid gun injects bacterial liquid that accounts for 2%-6% of the liquid volume in the mold at the vertical liquid level. 6 . The method for preparing an environment-friendly biocellulose facial mask according to claim 1 , wherein in the step 2) the inoculation temperature and the culture temperature are 30° C. 7 . 7. The environment-friendly biocellulose facial mask matrix prepared by the method of any one of claims 1-6.